FastQCFastQC Report
Thu 26 May 2016
SRR936521_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936521_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences766336
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT61240.7991272757641557No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42640.5564138967763487No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT42560.5553699682645732No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36020.47002881242692496No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC26920.35128194421246034No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT25920.3382328378152664No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA23170.3023477952229831No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22340.29151703691331216No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA20700.27011650242191415No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT16930.22092137130449307No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT16130.21048208618673794No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC14450.18855958743945214No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT13760.17955570402538834No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC13250.17290065976281946No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC13130.17133476699515618No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG11590.15123914314347753No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA10640.1388424920661433No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA9800.1278812426925004No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9390.12253110906965091No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG8350.10896003841656923No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA8130.10608923500918657No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC8100.10569776181727074No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG6800.036.3457832
GTACCTG8100.031.0992871
ACCTGGG10150.025.2864173
GTACATG57200.023.9307351
TACATGG56600.023.8485242
GAGTACT38150.023.60881812-13
ACATGGG55050.023.0522613
GGTAAGG2704.0017767E-1122.8843823
CATGGGG15950.022.0525594
GTACTTT42150.021.3119814-15
GTACACG1604.9630282E-520.793981
AGTACTT40450.019.68139312-13
CATGGGA38000.019.6383084
TACACTG4850.019.600915
TGGGACG1507.6861127E-419.0116426
ACTTTTT49650.018.6173316-17
ATGGGAT13900.018.123795
CATGGGT6300.018.1075064
GTAAGGT3156.7375368E-918.1075064
TAGCGCG800.001192106517.81061428-29
Produced by FastQC (version 0.11.5)