FastQCFastQC Report
Thu 26 May 2016
SRR936521_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936521_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences766336
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT81461.0629802071154166No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57540.7508455820945382No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53060.6923855854351094No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32210.4203117170536162No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT30780.4016514949056289No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT25060.3270106063136796No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA24500.3197031067312511No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC24350.31774574077167195No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA23700.30926382161349586No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT18720.24427927175547018No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC18080.23592784366126607No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC16940.22105186236846502No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT16470.21491878236178388No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG14770.1927353014865542No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14050.18333994488057456No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA13940.18190454317688326No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT12410.16193941038917656No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA10330.1347972690830132No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC9730.12696780524469684No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9420.12292258226156673No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9180.11979079672624018No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8000.10439285117755136No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG8000.10439285117755136No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG6850.036.7500842
ATCACGC753.078354E-431.6651653
TATCACG650.005844224729.2293852
ACGGACG350.008381017527.15042934-35
GTACCTG9500.026.5056631
GTATAGG2651.8189894E-1225.09971
TCTATAC1901.1903467E-824.9988163
ACCTGGG10350.024.3225173
CGGTATA1000.001653600323.7488752
GTACATG51650.023.7320771
TATAGGG2052.6677299E-823.1696342
GAGTACT51150.022.937112-13
TACATGG52100.022.6092932
GTAAGCG753.0367053E-522.1641794-95
ACATGGG51500.021.9504173
CTATACC1302.9412116E-421.9220394
ATAGTAC2405.9226295E-921.7698023
AGTACTT52050.021.67355212-13
GTACTTT55450.021.1148714-15
CATGGGT5400.020.2305224
Produced by FastQC (version 0.11.5)