##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936521_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 766336 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.943528687155503 31.0 31.0 34.0 30.0 34.0 2 32.06119639427092 31.0 31.0 34.0 30.0 34.0 3 32.15783285660598 33.0 31.0 34.0 30.0 34.0 4 35.89346057082011 37.0 35.0 37.0 35.0 37.0 5 35.70608062259896 37.0 35.0 37.0 33.0 37.0 6 35.84202621304493 37.0 35.0 37.0 35.0 37.0 7 35.79435260773342 37.0 35.0 37.0 35.0 37.0 8 35.78426173375647 37.0 35.0 37.0 33.0 37.0 9 37.40401860280608 39.0 37.0 39.0 34.0 39.0 10-11 37.2794955476449 39.0 37.0 39.0 34.0 39.0 12-13 37.19094365917822 39.0 37.0 39.0 33.0 39.0 14-15 38.212467507725066 40.0 37.5 41.0 33.0 41.0 16-17 38.16959336374645 40.0 37.5 41.0 33.0 41.0 18-19 38.40386136107399 40.0 38.0 41.0 34.0 41.0 20-21 38.46056364310172 40.0 38.0 41.0 34.0 41.0 22-23 38.367533301319526 40.0 38.0 41.0 34.0 41.0 24-25 38.23412706697845 40.0 37.5 41.0 33.5 41.0 26-27 37.986695131117415 40.0 37.0 41.0 33.0 41.0 28-29 37.89347949202438 40.0 37.0 41.0 33.0 41.0 30-31 37.81616092679973 40.0 37.0 41.0 33.0 41.0 32-33 37.608240380198765 40.0 36.0 41.0 33.0 41.0 34-35 37.380971271087354 39.0 36.0 41.0 33.0 41.0 36-37 37.08351493339737 39.0 35.5 41.0 31.5 41.0 38-39 36.88460022757641 39.0 35.0 41.0 31.0 41.0 40-41 36.65194770962084 39.0 35.0 41.0 31.0 41.0 42-43 36.35922624018707 39.0 35.0 40.0 30.0 41.0 44-45 36.01307194233339 39.0 35.0 40.0 30.0 41.0 46-47 35.50208589965759 39.0 35.0 40.0 27.5 41.0 48-49 35.00412090780023 38.0 35.0 40.0 25.5 41.0 50-51 34.64332746993486 38.0 34.0 40.0 24.0 41.0 52-53 34.38484294095541 38.0 34.0 40.0 23.5 41.0 54-55 34.12876531965091 38.0 34.0 40.0 23.0 41.0 56-57 33.80673164251712 38.0 33.0 40.0 22.0 41.0 58-59 33.48273146504927 37.5 33.0 40.0 20.5 41.0 60-61 33.08173242546351 37.0 32.5 40.0 19.5 41.0 62-63 32.70613673375647 36.0 32.0 39.0 18.0 40.5 64-65 32.299636843368965 36.0 31.0 39.0 15.5 40.0 66-67 31.818338822657424 35.0 31.0 38.5 12.5 40.0 68-69 31.53655119947386 35.0 31.0 38.0 9.5 40.0 70-71 31.077209605186237 35.0 30.5 37.5 7.5 40.0 72-73 30.610752333180223 34.5 30.0 37.0 6.5 39.0 74-75 30.091669319984966 34.0 29.0 36.5 2.0 39.0 76-77 28.693768138257894 32.5 28.0 35.0 2.0 37.0 78-79 29.44779117776015 34.0 29.0 35.0 2.0 37.5 80-81 29.211018404459665 34.0 29.0 35.0 2.0 37.0 82-83 28.847960946634373 34.0 29.0 35.0 2.0 37.0 84-85 28.422186221187573 34.0 28.5 35.0 2.0 36.0 86-87 28.066558924544847 33.0 27.5 35.0 2.0 36.0 88-89 27.610573560422583 33.0 26.5 35.0 2.0 35.0 90-91 27.08013521484049 33.0 26.0 35.0 2.0 35.0 92-93 26.615164236053115 32.5 25.0 34.5 2.0 35.0 94-95 26.030385496701186 32.0 24.5 34.0 2.0 35.0 96-97 25.231982446342073 32.0 21.0 34.0 2.0 35.0 98-99 24.295647731543344 31.5 10.0 34.0 2.0 35.0 100-101 21.11678363015701 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.26264638438949817 1101 2 0.2999812832025768 1101 3 0.2413501640439364 1101 4 0.17422123864586325 1101 5 0.0625814425970006 1101 6 0.10281114468000396 1101 7 0.1142294106967725 1101 8 0.07319257957453829 1101 9 0.11455277586743051 1101 10-11 0.16147297407230354 1101 12-13 0.07654712611071801 1101 14-15 0.21622576324214293 1101 16-17 0.37337971779770385 1101 18-19 0.2740612461261165 1101 20-21 0.156013345943947 1101 22-23 0.1398957660885145 1101 24-25 0.17037705591273777 1101 26-27 -0.03860120771578579 1101 28-29 -0.03152753377116113 1101 30-31 0.1723409674574654 1101 32-33 0.2904365210849136 1101 34-35 0.10653410404294306 1101 36-37 0.20427656404962136 1101 38-39 0.20582668421819506 1101 40-41 0.39350611983147843 1101 42-43 0.2642022505353836 1101 44-45 0.1731396033104673 1101 46-47 0.36516594301023275 1101 48-49 0.37662983787257787 1101 50-51 0.555251711530822 1101 52-53 0.4493835720498893 1101 54-55 0.4694264835526951 1101 56-57 0.48999753643748534 1101 58-59 0.4058571495149579 1101 60-61 0.35803462478084214 1101 62-63 0.4537663557542331 1101 64-65 0.40860830477839016 1101 66-67 -0.02687521613806254 1101 68-69 -0.1359447131638838 1101 70-71 0.2103837169872449 1101 72-73 0.3357089688358279 1101 74-75 0.3620350505208556 1101 76-77 0.11782922975919163 1101 78-79 0.0335806983958058 1101 80-81 0.45380222135877446 1101 82-83 0.6644539901387532 1101 84-85 0.6299363979703507 1101 86-87 0.4868332385111742 1101 88-89 0.3042361537437266 1101 90-91 0.3255133606881415 1101 92-93 0.5528686024070844 1101 94-95 0.6751216938598112 1101 96-97 0.6824201904038922 1101 98-99 1.147966330224481 1101 100-101 1.5228493400291967 1102 1 0.14521467562117607 1102 2 0.1943081074574522 1102 3 0.0802607498609973 1102 4 0.02801471514285936 1102 5 -0.12163016556008444 1102 6 0.013108110115169325 1102 7 0.029209667093155645 1102 8 0.04065512081974276 1102 9 -0.09587076499912683 1102 10-11 0.11050093966974828 1102 12-13 0.015080690236857208 1102 14-15 0.09634097933993502 1102 16-17 0.06790181440613452 1102 18-19 0.03398389447360728 1102 20-21 -0.12373079859004577 1102 22-23 -0.029222533256969996 1102 24-25 0.018425414218576464 1102 26-27 -0.2525126049138109 1102 28-29 -0.4599761733557415 1102 30-31 0.033450395704342384 1102 32-33 -0.03886785496312939 1102 34-35 0.10430929742208406 1102 36-37 -0.04775026561446083 1102 38-39 -0.08783318487900971 1102 40-41 0.030188112583140025 1102 42-43 0.043790069002412224 1102 44-45 0.24594103134096912 1102 46-47 0.16748811534754537 1102 48-49 0.06833211514850746 1102 50-51 -0.07037710872124592 1102 52-53 -0.15540628027877545 1102 54-55 -0.22460391618766806 1102 56-57 -0.15377592599557488 1102 58-59 -0.19036343792485866 1102 60-61 -0.14568961387335122 1102 62-63 -0.11894095208370459 1102 64-65 -0.04433376262031885 1102 66-67 -0.1467616228022024 1102 68-69 -0.15013197989185656 1102 70-71 -0.11155494261139154 1102 72-73 -0.0511093359188628 1102 74-75 -0.383003625305399 1102 76-77 -0.6524569172630414 1102 78-79 -0.388757160640278 1102 80-81 -0.19668677090606224 1102 82-83 -0.0037447441069353715 1102 84-85 -0.004716060513992204 1102 86-87 -0.019379235118943683 1102 88-89 -0.22627934363280744 1102 90-91 0.2253635256689357 1102 92-93 0.15336192203709587 1102 94-95 0.447393560576959 1102 96-97 0.693251044517595 1102 98-99 0.3096772622307533 1102 100-101 0.6228298668859047 1103 1 0.08675822649475862 1103 2 0.12456461653590623 1103 3 0.18426683071059813 1103 4 0.014276063207269374 1103 5 0.04331609171980233 1103 6 0.06817298678526384 1103 7 -0.03786488754884232 1103 8 0.06533073746927442 1103 9 0.22694312674462225 1103 10-11 0.09335893898458636 1103 12-13 0.05025326646159556 1103 14-15 0.11914791236494437 1103 16-17 0.10452555113104012 1103 18-19 -0.036421210014232486 1103 20-21 -0.0033123119507862953 1103 22-23 -0.09375554970095834 1103 24-25 -8.181195258600837E-4 1103 26-27 -0.018486076136831286 1103 28-29 -0.0720757793852016 1103 30-31 0.08314688851010033 1103 32-33 0.18818871406736548 1103 34-35 0.14441239351663882 1103 36-37 0.2905210815934893 1103 38-39 0.28056352632345494 1103 40-41 0.2458800233402485 1103 42-43 0.21487659264064973 1103 44-45 0.02073280506484565 1103 46-47 0.16763853072952628 1103 48-49 0.28425045190767406 1103 50-51 0.43770785188170436 1103 52-53 0.26237699310252083 1103 54-55 0.15611508004391794 1103 56-57 0.2089777995953881 1103 58-59 0.4693659214447834 1103 60-61 0.28596773881593407 1103 62-63 0.26740122417529477 1103 64-65 0.02649207670821596 1103 66-67 0.53804802947597 1103 68-69 0.5436276552571648 1103 70-71 0.5783881029521574 1103 72-73 0.6586146705902145 1103 74-75 0.46148132245068396 1103 76-77 0.3831197999346294 1103 78-79 0.3637506545361511 1103 80-81 0.8876673529377186 1103 82-83 0.841466051542259 1103 84-85 0.8701008716545573 1103 86-87 0.529294861318192 1103 88-89 0.3643622502349544 1103 90-91 0.6157326589337551 1103 92-93 0.8779727690737502 1103 94-95 0.9737949394738443 1103 96-97 0.7645254535617916 1103 98-99 1.058629707417463 1103 100-101 1.6277999979239333 1104 1 0.11533194444347572 1104 2 0.2656223088435965 1104 3 0.18135016404393411 1104 4 0.13486901192521827 1104 5 0.15823596351467728 1104 6 0.12934285858013794 1104 7 0.13420242014346684 1104 8 0.14358394259748053 1104 9 0.07069312674462225 1104 10-11 0.2281666312922752 1104 12-13 0.14387506133338945 1104 14-15 0.3311110533905861 1104 16-17 0.28081561523359966 1104 18-19 0.22902750793448234 1104 20-21 0.07049377779280519 1104 22-23 0.15783098876057977 1104 24-25 0.15640944457670258 1104 26-27 0.014246295658033148 1104 28-29 0.14225114369172331 1104 30-31 -0.08977779097708094 1104 32-33 0.16874961150325873 1104 34-35 0.10079861146535052 1104 36-37 0.3629810174909238 1104 38-39 0.019281475041402985 1104 40-41 0.18524701051973835 1104 42-43 0.37278524648679934 1104 44-45 0.33465107429562124 1104 46-47 0.5168933384218377 1104 48-49 0.520003657035879 1104 50-51 0.30591298008683054 1104 52-53 0.6431622495127769 1104 54-55 0.7289676441464792 1104 56-57 0.7459489534415411 1104 58-59 0.6044941265729875 1104 60-61 0.5262802388159358 1104 62-63 0.40450859597016375 1104 64-65 0.5203222049133487 1104 66-67 0.3565255935785352 1104 68-69 0.565053937308452 1104 70-71 0.4526509234649758 1104 72-73 0.4400409526414961 1104 74-75 0.6273386942455552 1104 76-77 0.5270060178833482 1104 78-79 0.36964007761307727 1104 80-81 0.6961369042197703 1104 82-83 0.6201759874396942 1104 84-85 0.580597666526355 1104 86-87 0.40673877157460225 1104 88-89 0.7094183399785443 1104 90-91 0.7432326589337528 1104 92-93 0.5205609100993946 1104 94-95 0.7244199394738438 1104 96-97 0.7442690433053762 1104 98-99 0.5675479766482319 1104 100-101 1.3563176261290621 1105 1 0.022388838167039182 1105 2 0.02619901608697006 1105 3 0.06898203834247596 1105 4 0.013984957707826595 1105 5 -0.12909201714663965 1105 6 -0.02559454969060937 1105 7 -0.07526317598880183 1105 8 0.0534725724861147 1105 9 -0.15691630086480757 1105 10-11 -0.059741509949198246 1105 12-13 -0.24704611625445239 1105 14-15 -0.08764747203461098 1105 16-17 -0.09060456951991824 1105 18-19 -0.05509404952039887 1105 20-21 -0.026230732265041468 1105 22-23 -0.1195306619344052 1105 24-25 -0.18742375925649668 1105 26-27 0.14233919041759435 1105 28-29 0.01248510725902463 1105 30-31 0.07895742442480014 1105 32-33 0.03519699128397491 1105 34-35 0.05418547151887765 1105 36-37 -0.07242259124601702 1105 38-39 0.16311122553781843 1105 40-41 0.10204058731331855 1105 42-43 0.024618104986323885 1105 44-45 0.055498868476746566 1105 46-47 0.0653850935802538 1105 48-49 -0.10458582755361334 1105 50-51 0.08821290238675061 1105 52-53 -0.21407853439468028 1105 54-55 -0.381756693244526 1105 56-57 -0.5724089849163931 1105 58-59 -0.21048817507598017 1105 60-61 -0.35612155691919867 1105 62-63 -0.22376214563391983 1105 64-65 -0.42045306919526126 1105 66-67 -0.22016956311661673 1105 68-69 -0.0766830910952443 1105 70-71 -0.3614196972723107 1105 72-73 -0.2511461318789223 1105 74-75 -0.4861845726110481 1105 76-77 -0.5183725541618891 1105 78-79 -0.3398565985502664 1105 80-81 -0.2905435757940431 1105 82-83 -0.35024445631407986 1105 84-85 -0.398561445966088 1105 86-87 -0.1973447991756352 1105 88-89 -0.014174856947985148 1105 90-91 0.24184096420872692 1105 92-93 -0.1219237657186163 1105 94-95 -0.07519846681122999 1105 96-97 -0.06987409750442808 1105 98-99 0.22395132638491333 1105 100-101 0.6063616561730889 1106 1 0.024317188758910646 1106 2 0.08969826433465045 1106 3 0.037057711213748235 1106 4 0.10415420786135599 1106 5 -0.07250544916069401 1106 6 0.003019684898468711 1106 7 -0.08871001333501027 1106 8 -0.038619734228838354 1106 9 -0.1896056153937451 1106 10-11 -0.14952628114119904 1106 12-13 -0.0649983058654513 1106 14-15 0.011374720541049044 1106 16-17 -0.05407311239097368 1106 18-19 -0.21421209051737122 1106 20-21 -0.14384886855456358 1106 22-23 -0.15372017234247437 1106 24-25 -0.3900673333623388 1106 26-27 -0.2099070667028755 1106 28-29 -0.3346543957374024 1106 30-31 -0.20302646054650353 1106 32-33 -0.2345510658068477 1106 34-35 -0.090051049879591 1106 36-37 -0.21483465740021757 1106 38-39 0.14235597915364906 1106 40-41 0.04474991327735012 1106 42-43 0.09992965867838421 1106 44-45 0.08996433022208095 1106 46-47 -0.032253371786190144 1106 48-49 -0.010659139287298558 1106 50-51 -0.1773078713887415 1106 52-53 0.11078501197044233 1106 54-55 -0.01589749857243561 1106 56-57 -0.014312294744236453 1106 58-59 -0.08055546220301579 1106 60-61 -0.08043359766205782 1106 62-63 -0.22502212488131335 1106 64-65 0.09514380626796992 1106 66-67 -0.0601634485114495 1106 68-69 -0.027068099459814476 1106 70-71 0.2583802413169387 1106 72-73 0.12345350706820213 1106 74-75 0.11288658974627808 1106 76-77 -0.2438750900024793 1106 78-79 -0.010905398922965048 1106 80-81 -0.020027222533979483 1106 82-83 0.004309998083137145 1106 84-85 -0.2444628075907218 1106 86-87 0.04215846194712114 1106 88-89 0.13153009614690347 1106 90-91 0.2985942941538795 1106 92-93 0.10449596089764768 1106 94-95 0.376291008656235 1106 96-97 0.2655867743165068 1106 98-99 0.5475939369772114 1106 100-101 0.7529474035843116 1107 1 0.03647413558567081 1107 2 0.1577085559298439 1107 3 0.07839561858938993 1107 4 0.054711669267874186 1107 5 0.048335031113744265 1107 6 0.024138895876170352 1107 7 0.03135859729964352 1107 8 -0.011089717076174566 1107 9 0.06539009644158966 1107 10-11 0.04241196928761326 1107 12-13 0.03756387252219895 1107 14-15 0.046183897213431635 1107 16-17 -0.10025664583865534 1107 18-19 -0.07884545243847185 1107 20-21 0.045977460776484236 1107 22-23 -0.14063054970095834 1107 24-25 -0.05129160437434166 1107 26-27 0.021996878408621967 1107 28-29 -0.0951818399912554 1107 30-31 -0.15335879330807956 1107 32-33 2.152292188810634E-4 1107 34-35 -0.02533192466518841 1107 36-37 0.17068206644196948 1107 38-39 0.12601807177800595 1107 40-41 -0.10984914332642148 1107 42-43 -0.3165154528139027 1107 44-45 -0.2118903009957549 1107 46-47 -0.0781474541189553 1107 48-49 -0.33440485112263474 1107 50-51 -0.28691336023950953 1107 52-53 -0.5918843705338404 1107 54-55 -0.337445526016694 1107 56-57 -0.5833517458591615 1107 58-59 -0.43650529067642196 1107 60-61 -0.2973182460325532 1107 62-63 -0.1804680940065282 1107 64-65 -0.18761777177662964 1107 66-67 -0.09708454628160368 1107 68-69 0.04334356434807418 1107 70-71 -0.29699068492663017 1107 72-73 -0.2613663900158478 1107 74-75 -0.3047592078523458 1107 76-77 -0.5949578515805207 1107 78-79 -0.2547626030396053 1107 80-81 -0.18605423797137277 1107 82-83 -0.17761538785168085 1107 84-85 -0.44552412834543986 1107 86-87 -0.5459418811060495 1107 88-89 -0.35206767400747196 1107 90-91 -0.1986612804601826 1107 92-93 -0.13315412486564426 1107 94-95 0.04263963644354263 1107 96-97 0.5327072717436074 1107 98-99 0.9551732680235219 1107 100-101 1.0043624979239318 1108 1 0.0031258007521870468 1108 2 -0.015266241549902304 1108 3 -0.054491420114480604 1108 4 -0.0042779796970222606 1108 5 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-1.260344475369834 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 3.0 4 7.0 5 21.0 6 53.0 7 126.0 8 346.0 9 591.0 10 1065.0 11 1824.0 12 2813.0 13 3723.0 14 4962.0 15 6812.0 16 9117.0 17 11941.0 18 10186.0 19 5858.0 20 4909.0 21 5072.0 22 5371.0 23 5830.0 24 6765.0 25 7944.0 26 9439.0 27 11762.0 28 14637.0 29 18079.0 30 22028.0 31 27165.0 32 34364.0 33 44306.0 34 58375.0 35 79987.0 36 111921.0 37 144672.0 38 88944.0 39 5318.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.476902833691 19.626978283912134 14.843068761519865 20.053050120876996 2 15.585715497497668 22.623827949131197 39.15997311705175 22.630483436319384 3 21.21214493854323 26.55175248691458 30.671620269055254 21.564482305486937 4 12.40103819367871 17.406969051232434 38.52147440205342 31.670518353035437 5 12.151668143400176 39.751230212532185 33.89005534162131 14.207046302446333 6 29.184886505249008 37.61083599209223 17.554463778895652 15.649813723763106 7 27.03278979455487 35.07651995991314 20.742468055787537 17.14822218974445 8 26.435793176883248 33.03263842492066 19.75647757641557 20.775090821780527 9 26.65111174618337 17.559182427115672 21.319506157373517 34.47019966932744 10-11 24.879621679319982 26.965461535048686 26.827793103268288 21.32712368236304 12-13 27.72390988471669 24.825091556569333 27.369754133176393 20.081244425537577 14-15 23.427391756256732 25.925778515217967 28.2456484902739 22.401181238251397 16-17 20.447519486162964 29.1121252021041 28.90900976493939 21.531345546793542 18-19 20.13783771087356 27.969063178553533 31.963981856522466 19.929117254050443 20-21 21.970454213295472 25.707196321195923 31.66457011023885 20.65777935526975 22-23 21.085310162720187 26.091668509902256 31.679902076886084 21.143119250491473 24-25 21.774610283351993 26.053598599029655 32.13129921208468 20.040491905533674 26-27 21.500559837347026 25.50150464443151 33.00821877764931 19.989716740572156 28-29 20.270882217065957 27.049658477254873 32.67754093183378 20.00191837384539 30-31 20.732535629488552 26.22374725067514 32.17344610540313 20.870271014433182 32-33 20.727619122185466 26.550725205034354 32.08420599346239 20.637449679317793 34-35 21.80483116249236 25.818575943110282 32.044966783229334 20.331626111168024 36-37 21.829148207675157 25.489428373239992 32.48358348815068 20.19783993093417 38-39 21.17788541535482 26.153386374892413 32.696251702273855 19.972476507478913 40-41 21.003672114375792 26.573371820502246 32.1530543535565 20.269901711565463 42-43 20.593005912573254 27.13252593846278 31.042836469263364 21.231631679700598 44-45 21.293497345449158 26.336524101051058 30.727412829143752 21.642565724356032 46-47 22.15870203063802 27.26703904490918 29.917640511238226 20.656618413214577 48-49 22.204149009398723 27.274435538330543 29.55948990477309 20.961925547497643 50-51 21.194741914162996 27.84447058654656 30.331537096882588 20.629250402407855 52-53 21.835109367700447 28.305955522148885 29.048731981929077 20.81020312822159 54-55 20.866664230738113 27.662317664222908 29.501073983500063 21.969944121538916 56-57 22.040467191118125 27.707191927179675 28.87458250388706 21.37775837781514 58-59 23.037097740259707 27.0402843694663 28.604172892250958 21.318444998023036 60-61 21.65044913842546 27.391277964714888 30.324265741703154 20.634007155156496 62-63 21.860094559624372 27.274957620427802 29.984509760677756 20.88043805927007 64-65 21.404479535407255 28.473989005073324 29.281545162395396 20.839986297124028 66-67 21.628104086017576 27.625427618250352 28.845889090359822 21.900579205372246 68-69 21.328565015633398 28.093664688349858 29.00700501625978 21.570765279756966 70-71 22.13226313262068 28.019563220310673 28.55170577918824 21.29646786788041 72-73 21.995143059220226 27.238211935912393 29.291646114055897 21.474998890811484 74-75 21.294594968458252 29.155563327530455 28.49954194647951 21.050299757531782 76-77 21.82693687933082 27.75117119703514 28.585820359906567 21.836071563727476 78-79 21.734824041419675 27.876067218885492 28.71196426998476 21.67714446971007 80-81 22.165738502344063 28.07277852270169 28.438824346940972 21.322658628013272 82-83 21.882193732937314 27.870902997812845 28.92232986903165 21.324573400218195 84-85 21.444627505994813 28.176565584066033 28.68335970507153 21.695447204867623 86-87 21.530293460558735 28.40922140560404 28.22952770701967 21.830957426817555 88-89 21.64853736680348 28.2176951537021 27.745463324872265 22.388304154622155 90-91 21.62584723725775 27.533522438451115 28.27173713736092 22.568893186930215 92-93 21.924546472389036 28.186959596829546 28.28907018683954 21.599423743941873 94-95 21.51371390951958 27.972173522697098 28.366721234783622 22.147391332999707 96-97 20.841309672477486 27.849070046938063 28.994531069553673 22.315089211030774 98-99 21.28662449473612 28.429710005448207 27.571242614746694 22.71242288506898 100-101 20.78521261852377 28.246239733160035 27.902119529898133 23.066428118418063 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 528.0 1 416.0 2 442.0 3 550.5 4 417.5 5 361.5 6 583.0 7 1059.5 8 1602.0 9 1912.5 10 1792.0 11 1510.0 12 1500.0 13 1571.5 14 1584.5 15 1714.5 16 1858.0 17 1958.5 18 2087.5 19 2258.5 20 2373.5 21 2563.5 22 2948.5 23 3347.0 24 3837.5 25 4468.5 26 5352.5 27 6540.0 28 7644.5 29 9036.0 30 10608.0 31 12125.5 32 14300.5 33 16104.0 34 17350.5 35 18560.5 36 20279.5 37 21825.5 38 22094.0 39 22102.5 40 22368.0 41 22982.0 42 23139.0 43 23441.5 44 24547.0 45 25398.0 46 25700.5 47 30799.5 48 45911.5 49 44403.5 50 28784.0 51 22889.5 52 20256.5 53 19243.0 54 18614.5 55 17325.0 56 15602.5 57 14150.5 58 13371.0 59 12047.0 60 10144.5 61 8496.5 62 6948.5 63 5362.0 64 3854.5 65 2855.0 66 2165.5 67 1593.0 68 1144.5 69 829.0 70 615.5 71 503.0 72 442.0 73 404.0 74 299.0 75 239.5 76 191.5 77 118.5 78 79.0 79 52.0 80 36.0 81 25.5 82 18.0 83 14.5 84 10.0 85 6.0 86 2.5 87 2.0 88 1.5 89 1.5 90 1.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03497160514447971 2 0.0066550442625688995 3 0.00326227659929848 4 6.52455319859696E-4 5 6.52455319859696E-4 6 1.304910639719392E-4 7 0.0 8 0.0 9 0.0024793302154668445 10-11 2.609821279438784E-4 12-13 8.481919158176049E-4 14-15 0.002805557875396693 16-17 0.0030012944713546015 18-19 0.0 20-21 0.0 22-23 0.0026750668114247536 24-25 0.001304910639719392 26-27 0.005480624686821447 28-29 0.00809044596626023 30-31 0.0015006472356773007 32-33 1.304910639719392E-4 34-35 0.010635021713713045 36-37 0.014419262568899282 38-39 0.010635021713713045 40-41 0.002609821279438784 42-43 3.914731919158176E-4 44-45 0.0013701561717053617 46-47 6.52455319859696E-5 48-49 0.0011091740437614832 50-51 0.0015006472356773007 52-53 0.0028708034073826627 54-55 0.003914731919158176 56-57 0.0015006472356773007 58-59 0.0011744195757474529 60-61 0.004632432771003842 62-63 0.006133080006681143 64-65 0.009591093201937531 66-67 0.003588504259228328 68-69 0.003653749791214298 70-71 0.0 72-73 0.001304910639719392 74-75 0.00652455319859696 76-77 0.0033927676632704196 78-79 0.004371450643059964 80-81 0.005023905962919659 82-83 0.005741606814765325 84-85 0.006328816602639051 86-87 0.0041757140471020545 88-89 0.010569776181727076 90-91 0.012461896609320195 92-93 0.001304910639719392 94-95 0.0020226114915650574 96-97 9.134374478035745E-4 98-99 0.003718995323200267 100-101 0.004436696175045933 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 766336.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.52164626710699 #Duplication Level Percentage of deduplicated Percentage of total 1 81.79131408708173 47.047710366389595 2 10.962216836623414 12.611295183591531 3 3.0701702518782303 5.29803741625003 4 1.2961027686486126 2.9821585993609396 5 0.6876937125013949 1.9778637235309402 6 0.4329223924450491 1.494144523160027 7 0.29220298130726 1.1765597570265198 8 0.21294561930036018 0.9799186070020277 9 0.15525252794023545 0.803734289482713 >10 0.9785145297156685 10.474132709993416 >50 0.07317138498680394 2.9578860096145467 >100 0.0393123110526151 4.186558448714223 >500 0.004090298259299791 1.6264431225960923 >1k 0.0034085818827498254 3.8731122852724265 >5k 6.817163765499651E-4 2.5104449580149923 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8146 1.0629802071154166 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5754 0.7508455820945382 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5306 0.6923855854351094 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3221 0.4203117170536162 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3078 0.4016514949056289 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2506 0.3270106063136796 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2450 0.3197031067312511 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2435 0.31774574077167195 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2370 0.30926382161349586 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1872 0.24427927175547018 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1808 0.23592784366126607 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1694 0.22105186236846502 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1647 0.21491878236178388 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1477 0.1927353014865542 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1405 0.18333994488057456 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1394 0.18190454317688326 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1241 0.16193941038917656 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1033 0.1347972690830132 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 973 0.12696780524469684 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 942 0.12292258226156673 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 918 0.11979079672624018 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 800 0.10439285117755136 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 800 0.10439285117755136 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 1.304910639719392E-4 0.0 0.0 0.0 0.0 7 1.304910639719392E-4 0.0 0.0 0.0 0.0 8 1.304910639719392E-4 0.0 0.0 0.0 0.0 9 1.304910639719392E-4 0.0 0.0 0.0 0.0 10-11 1.304910639719392E-4 0.0 0.0 0.0 0.0 12-13 1.957365959579088E-4 0.0 0.0 0.0 0.0 14-15 2.609821279438784E-4 0.0 0.0 0.0 0.0 16-17 2.609821279438784E-4 0.0 0.0 0.0 0.0 18-19 2.609821279438784E-4 0.0 0.0 0.0 0.0 20-21 2.609821279438784E-4 0.0 0.0 1.304910639719392E-4 0.0 22-23 2.609821279438784E-4 0.0 0.0 6.524553198596959E-4 0.0 24-25 2.609821279438784E-4 0.0 0.0 0.0015006472356773007 0.0 26-27 2.609821279438784E-4 0.0 0.0 0.002609821279438784 0.0 28-29 2.609821279438784E-4 0.0 0.0 0.0034580131952563885 0.0 30-31 2.609821279438784E-4 0.0 0.0 0.007698972774344413 0.0 32-33 2.609821279438784E-4 0.0 0.0 0.013962543844997493 0.0 34-35 2.609821279438784E-4 0.0 0.0 0.020095623851678637 1.304910639719392E-4 36-37 2.609821279438784E-4 0.0 0.0 0.027533614498079173 1.304910639719392E-4 38-39 3.914731919158176E-4 0.0 0.0 0.035102096208451644 1.304910639719392E-4 40-41 3.914731919158176E-4 0.0 0.0 0.045541381326206776 1.304910639719392E-4 42-43 4.567187239017872E-4 0.0 0.0 0.060091134959078 1.304910639719392E-4 44-45 7.177008518456656E-4 0.0 0.0 0.07327073242024386 1.304910639719392E-4 46-47 7.829463838316352E-4 0.0 0.0 0.09003883414063804 1.304910639719392E-4 48-49 7.829463838316352E-4 0.0 0.0 0.10204401202605645 1.304910639719392E-4 50-51 7.829463838316352E-4 0.0 0.0 0.120182269918156 1.304910639719392E-4 52-53 7.829463838316352E-4 0.0 0.0 0.1622656380491064 1.304910639719392E-4 54-55 7.829463838316352E-4 0.0 0.0 0.21465780023383998 1.304910639719392E-4 56-57 7.829463838316352E-4 0.0 0.0 0.2585680432603975 2.609821279438784E-4 58-59 7.829463838316352E-4 0.0 0.0 0.3267496241857357 2.609821279438784E-4 60-61 7.829463838316352E-4 0.0 0.0 0.41535305662268246 2.609821279438784E-4 62-63 7.829463838316352E-4 0.0 0.0 0.4687239017872056 2.609821279438784E-4 64-65 7.829463838316352E-4 0.0 0.0 0.5091108860865208 2.609821279438784E-4 66-67 0.0010439285117755136 0.0 0.0 0.6296193836646067 2.609821279438784E-4 68-69 0.0011744195757474529 0.0 0.0 0.7552170327375981 2.609821279438784E-4 70-71 0.0011744195757474529 0.0 0.0 0.8076744404543177 2.609821279438784E-4 72-73 0.0012396651077334225 0.0 0.0 0.9102404167362619 2.609821279438784E-4 74-75 0.001304910639719392 0.0 0.0 1.057956301152497 2.609821279438784E-4 76-77 0.001304910639719392 0.0 0.0 1.2046935025889427 2.609821279438784E-4 78-79 0.001304910639719392 0.0 0.0 1.3401432269918157 2.609821279438784E-4 80-81 0.0016963838316352096 0.0 0.0 1.4988203607816937 2.609821279438784E-4 82-83 0.0016963838316352096 0.0 0.0 1.6638915567061967 2.609821279438784E-4 84-85 0.0016963838316352096 0.0 0.0 1.858975697344246 2.609821279438784E-4 86-87 0.0016963838316352096 0.0 0.0 2.210322887088692 3.2622765992984797E-4 88-89 0.0016963838316352096 0.0 0.0 2.6364414564890595 3.914731919158176E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 685 0.0 36.750084 2 ATCACGC 75 3.078354E-4 31.665165 3 TATCACG 65 0.0058442247 29.229385 2 ACGGACG 35 0.0083810175 27.150429 34-35 GTACCTG 950 0.0 26.505663 1 GTATAGG 265 1.8189894E-12 25.0997 1 TCTATAC 190 1.1903467E-8 24.998816 3 ACCTGGG 1035 0.0 24.322517 3 CGGTATA 100 0.0016536003 23.748875 2 GTACATG 5165 0.0 23.732077 1 TATAGGG 205 2.6677299E-8 23.169634 2 GAGTACT 5115 0.0 22.9371 12-13 TACATGG 5210 0.0 22.609293 2 GTAAGCG 75 3.0367053E-5 22.16417 94-95 ACATGGG 5150 0.0 21.950417 3 CTATACC 130 2.9412116E-4 21.922039 4 ATAGTAC 240 5.9226295E-9 21.769802 3 AGTACTT 5205 0.0 21.673552 12-13 GTACTTT 5545 0.0 21.11487 14-15 CATGGGT 540 0.0 20.230522 4 >>END_MODULE