FastQCFastQC Report
Thu 26 May 2016
SRR936520_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936520_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences797069
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT64270.8063291885646036No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43230.5423620790671824No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT42960.5389746684415025No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36270.4550421607163244No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC26830.3366082484703332No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT26370.3308371044413972No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA23300.29232099103088943No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22230.27889680818097307No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA21420.26873457630393355No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT17700.22206358546123361No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT16690.20939216052813497No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT15000.18818947920443524No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC14180.1779017876745928No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC13950.1750162156601248No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC13420.16836685406156807No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG11820.14829330961309498No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA11330.1421457866257501No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA9870.12382867731651839No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG9210.11554834023152324No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8910.11178455064743455No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA8380.10513518904887782No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCACGC550.002568897634.573533
TACCTGG6750.030.9822852
GTACCCG953.4746816E-530.0187231
GTACCTG7900.028.277131
GTAAGGT2700.026.4103344
GAGTACT39450.024.16245312-13
GTACATG57150.024.118281
TACATGG57800.023.600362
ACATGGG57050.022.5818773
GTACTTT42400.022.36359414-15
TACCCGG1353.778578E-421.1242872
CATGGGT6250.020.5366764
TAAGGTG3503.6379788E-1220.3736865
AGTACTT41350.020.00540712-13
GTATAGG2151.0715103E-619.896131
GCACCGC1200.00473990919.8077536
TAGGACA3603.6379788E-1219.807754
ATGGGAT13200.019.4476095
CATGGGA37700.019.4189514
CATGGGG16250.019.015444
Produced by FastQC (version 0.11.5)