##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936520_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 797069 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.943393859251834 31.0 31.0 34.0 30.0 34.0 2 32.07983248627158 33.0 31.0 34.0 30.0 34.0 3 32.15438312116015 33.0 31.0 34.0 30.0 34.0 4 35.85006191433866 37.0 35.0 37.0 35.0 37.0 5 35.71205002327277 37.0 35.0 37.0 33.0 37.0 6 35.831566652322444 37.0 35.0 37.0 35.0 37.0 7 35.77768800442622 37.0 35.0 37.0 33.0 37.0 8 35.7659198889933 37.0 35.0 37.0 33.0 37.0 9 37.378051335580736 39.0 37.0 39.0 34.0 39.0 10-11 37.27216903429941 39.0 37.0 39.0 34.0 39.0 12-13 37.176226901309676 39.0 37.0 39.0 33.0 39.0 14-15 38.225577083037976 40.0 37.5 41.0 33.0 41.0 16-17 38.160711933345794 40.0 37.5 41.0 33.0 41.0 18-19 38.38355085946135 40.0 38.0 41.0 33.5 41.0 20-21 38.423192973255766 40.0 38.0 41.0 34.0 41.0 22-23 38.34699818961721 40.0 38.0 41.0 34.0 41.0 24-25 38.19304790425923 40.0 37.5 41.0 33.5 41.0 26-27 37.96657692119503 40.0 37.0 41.0 33.0 41.0 28-29 37.874388541017154 40.0 37.0 41.0 33.0 41.0 30-31 37.79938562408022 40.0 37.0 41.0 33.0 41.0 32-33 37.57746506262319 40.0 36.0 41.0 33.0 41.0 34-35 37.351784475371645 39.0 36.0 41.0 32.5 41.0 36-37 37.102054527274305 39.0 35.5 41.0 31.5 41.0 38-39 36.893169851041755 39.0 35.0 41.0 31.0 41.0 40-41 36.703181280416125 39.0 35.0 41.0 31.0 41.0 42-43 36.366359123237764 39.0 35.0 40.5 30.0 41.0 44-45 35.99060181740853 39.0 35.0 40.0 30.0 41.0 46-47 35.47306193064841 39.0 35.0 40.0 27.5 41.0 48-49 34.95201858308377 38.0 34.5 40.0 25.5 41.0 50-51 34.614863957825484 38.0 34.0 40.0 24.0 41.0 52-53 34.2703423417546 38.0 34.0 40.0 23.0 41.0 54-55 34.10749633971463 38.0 34.0 40.0 23.0 41.0 56-57 33.78690364322286 38.0 33.0 40.0 22.0 41.0 58-59 33.444850445820876 37.0 33.0 40.0 20.5 41.0 60-61 32.994168635337715 37.0 32.0 39.5 18.0 41.0 62-63 32.69221046107677 36.0 32.0 39.0 18.0 40.5 64-65 32.27298640393742 36.0 31.0 39.0 15.5 40.0 66-67 31.833673119893007 35.0 31.0 38.5 12.5 40.0 68-69 31.48017361106755 35.0 31.0 38.0 9.0 40.0 70-71 31.093579100429196 35.0 30.5 37.5 7.5 40.0 72-73 30.623595322362306 34.5 30.0 37.0 7.0 39.0 74-75 30.133113318922202 34.0 29.0 36.5 2.0 39.0 76-77 28.672925430546165 32.5 27.5 35.0 2.0 37.0 78-79 29.39712873038595 34.0 29.0 35.0 2.0 37.5 80-81 29.133135274361443 34.0 29.0 35.0 2.0 37.0 82-83 28.809194687034623 34.0 29.0 35.0 2.0 37.0 84-85 28.436233249568104 34.0 28.5 35.0 2.0 36.0 86-87 28.0822638943429 33.5 27.5 35.0 2.0 36.0 88-89 27.567305339939203 33.0 26.5 35.0 2.0 35.0 90-91 27.055233612146502 33.0 25.5 35.0 2.0 35.0 92-93 26.451236342148547 32.0 25.0 34.5 2.0 35.0 94-95 25.92804512532792 32.0 24.0 34.0 2.0 35.0 96-97 25.19546551176874 32.0 20.0 34.0 2.0 35.0 98-99 24.348597172892184 31.5 10.0 34.0 2.0 35.0 100-101 21.224038947694616 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3167733668248296 1101 2 0.32050402094431973 1101 3 0.31043899917575857 1101 4 0.25628053797515093 1101 5 0.24854153131876444 1101 6 0.32371704624848974 1101 7 0.262551875984677 1101 8 0.2542950883140449 1101 9 0.2115767032260223 1101 10-11 0.34762507596168035 1101 12-13 0.2801489080893731 1101 14-15 0.45228923187025316 1101 16-17 0.4559898322108822 1101 18-19 0.33384443929818275 1101 20-21 0.34126493410652614 1101 22-23 0.3338146377872775 1101 24-25 0.40383580722402 1101 26-27 0.3583252272063646 1101 28-29 0.4276304496832779 1101 30-31 0.4001167388396354 1101 32-33 0.14769520189013008 1101 34-35 0.2023925248684293 1101 36-37 0.3889395964987514 1101 38-39 0.4134356938975827 1101 40-41 0.34104458841756013 1101 42-43 0.33807232369330364 1101 44-45 0.621349671670167 1101 46-47 0.39875644587522885 1101 48-49 0.27269912866173485 1101 50-51 0.1463975912167257 1101 52-53 0.2915663342336927 1101 54-55 0.43764214270412083 1101 56-57 0.3921878126334448 1101 58-59 0.1980920843014644 1101 60-61 0.36661453191425863 1101 62-63 0.4653507564381343 1101 64-65 0.6110923554222367 1101 66-67 0.33409857363214357 1101 68-69 0.17717604055410519 1101 70-71 0.24625869949213808 1101 72-73 0.3903956952319092 1101 74-75 0.47871663107396145 1101 76-77 0.46264826391663405 1101 78-79 0.6227192354765982 1101 80-81 0.45174192012706627 1101 82-83 0.6885518218908793 1101 84-85 0.5336267156505237 1101 86-87 0.37860078151328835 1101 88-89 0.17975599039609236 1101 90-91 0.5219789306037796 1101 92-93 0.29083198499051477 1101 94-95 0.3633981251363885 1101 96-97 0.246688603237601 1101 98-99 0.3412130814274654 1101 100-101 0.3857414216417503 1102 1 0.31109118310165584 1102 2 0.2734657414534851 1102 3 0.16636933325966652 1102 4 0.14967468346478796 1102 5 0.19757190676106973 1102 6 0.13564894276060357 1102 7 0.21324577791932597 1102 8 0.10521416372418457 1102 9 0.1368696585130067 1102 10-11 0.14652448315803923 1102 12-13 0.15721797660643944 1102 14-15 0.22016180206463787 1102 16-17 0.3131333458361709 1102 18-19 0.2286126025259776 1102 20-21 -0.014674590731480919 1102 22-23 0.0887925799774365 1102 24-25 0.18132879927402 1102 26-27 0.1936269137097426 1102 28-29 0.12129687126316924 1102 30-31 0.15921328771147358 1102 32-33 0.28523368817293004 1102 34-35 0.2593809445182558 1102 36-37 0.21483339733511286 1102 38-39 0.002540054910397771 1102 40-41 0.1841618624399004 1102 42-43 0.5234647688934331 1102 44-45 0.5245583936958056 1102 46-47 0.44297086616841597 1102 48-49 0.6962849013743835 1102 50-51 0.689682596501406 1102 52-53 0.7124155599126638 1102 54-55 0.5910990150904993 1102 56-57 0.37720918113673463 1102 58-59 0.604614073180656 1102 60-61 0.447845863194992 1102 62-63 0.3089149308786432 1102 64-65 0.3870458961602594 1102 66-67 0.3942566546026889 1102 68-69 0.22444638658599914 1102 70-71 0.43428314689804637 1102 72-73 0.3685688030854095 1102 74-75 0.19651222870274765 1102 76-77 0.2957257879274806 1102 78-79 0.30398732977373655 1102 80-81 0.203668531955568 1102 82-83 0.10116153651796367 1102 84-85 0.2655852653495252 1102 86-87 0.47687981280780534 1102 88-89 0.4848304355043176 1102 90-91 0.5878720421335792 1102 92-93 0.610083168300104 1102 94-95 0.45396726258411135 1102 96-97 0.918154758285624 1102 98-99 0.7983577438970251 1102 100-101 1.4571397948782767 1103 1 0.11123875369325376 1103 2 0.25745763608367866 1103 3 0.18687944294646286 1103 4 0.1564298924864147 1103 5 0.22898682240354873 1103 6 0.18490007707186606 1103 7 0.09071593341908368 1103 8 0.06445164224935951 1103 9 0.11168134081774639 1103 10-11 0.24474262332807228 1103 12-13 0.22393750438055093 1103 14-15 0.278598655066709 1103 16-17 0.20684727914307643 1103 18-19 0.23515196202555444 1103 20-21 0.13242685854387304 1103 22-23 0.2037621848406559 1103 24-25 0.22960750946299413 1103 26-27 0.09038256324060256 1103 28-29 0.29171337273886877 1103 30-31 0.2655434053274419 1103 32-33 0.3513662373998372 1103 34-35 0.4071222334482627 1103 36-37 0.19636394665011636 1103 38-39 0.20412809067974536 1103 40-41 0.5383389912007388 1103 42-43 0.4664926090766812 1103 44-45 0.6654673844891619 1103 46-47 0.7685776676309075 1103 48-49 0.5643898306726527 1103 50-51 0.8197572628156706 1103 52-53 0.7100764559084354 1103 54-55 0.6128106136984854 1103 56-57 0.30211116783335257 1103 58-59 0.5397237599785853 1103 60-61 0.5293103361256968 1103 62-63 0.6186347670092118 1103 64-65 0.6005816434841691 1103 66-67 0.5917105112609882 1103 68-69 0.5984541395484229 1103 70-71 0.6575067934334413 1103 72-73 0.6469254834078626 1103 74-75 0.6388848114083565 1103 76-77 0.6996132818704801 1103 78-79 0.41573020453695975 1103 80-81 0.6551167060448151 1103 82-83 0.6552869054444415 1103 84-85 0.9056055287740428 1103 86-87 0.7242301497973678 1103 88-89 0.851731673335248 1103 90-91 0.9938497523666072 1103 92-93 0.9995605041002911 1103 94-95 0.7239718879310146 1103 96-97 1.1056894483873805 1103 98-99 1.1169923855427655 1103 100-101 1.3701083865702728 1104 1 0.21400457587997224 1104 2 0.2944068556055086 1104 3 0.19172467889399059 1104 4 0.20308247016912162 1104 5 0.2702787135060163 1104 6 0.08736607880721436 1104 7 0.10700846883484161 1104 8 0.03192026419209526 1104 9 0.2880041160039539 1104 10-11 0.2125989071008334 1104 12-13 0.13965595665411712 1104 14-15 0.24796263194015467 1104 16-17 0.24957061960680704 1104 18-19 0.09067007581987241 1104 20-21 0.03605155034735219 1104 22-23 0.265039600371189 1104 24-25 0.2522390138056636 1104 26-27 0.18330864761986732 1104 28-29 0.35529528213983497 1104 30-31 0.34809604572330244 1104 32-33 0.3350747758395727 1104 34-35 0.18095108665651338 1104 36-37 0.11396379373309884 1104 38-39 0.26752637079280817 1104 40-41 0.4433081500712177 1104 42-43 0.32282470532850027 1104 44-45 0.25536090097929076 1104 46-47 0.656858038411734 1104 48-49 0.7219051008976436 1104 50-51 0.5020689300407355 1104 52-53 0.4023202125126488 1104 54-55 0.46635283340073386 1104 56-57 0.4027808241136981 1104 58-59 0.39429580709077783 1104 60-61 0.3793281621267468 1104 62-63 0.43090442500771786 1104 64-65 0.46068189863222386 1104 66-67 0.4254476142566084 1104 68-69 0.3297765711008722 1104 70-71 0.4000993382991247 1104 72-73 0.4517726522952614 1104 74-75 0.2927844102159476 1104 76-77 0.18416050144385565 1104 78-79 0.18384077728851977 1104 80-81 0.24892764734357797 1104 82-83 0.32202981585053436 1104 84-85 0.6721536417951874 1104 86-87 0.5711413118808188 1104 88-89 0.5069832561982324 1104 90-91 0.615169649620114 1104 92-93 0.6678809082135864 1104 94-95 0.7863478576225162 1104 96-97 0.9127208393309125 1104 98-99 0.7366048525754003 1104 100-101 1.275734744997461 1105 1 0.1218224810193611 1105 2 0.07479949820165643 1105 3 0.15424757124905142 1105 4 0.08673179910240236 1105 5 0.18343816815924185 1105 6 0.07155376515319745 1105 7 0.06180767965683742 1105 8 0.0057698957025920095 1105 9 -0.009158559061731353 1105 10-11 -0.055696176461786706 1105 12-13 0.15467975585608684 1105 14-15 0.09740060803478912 1105 16-17 0.23733150398161484 1105 18-19 0.20164712595744305 1105 20-21 0.17967508943909394 1105 22-23 0.0939540824952374 1105 24-25 0.2647192180010549 1105 26-27 0.26962609712398233 1105 28-29 0.12017973657188463 1105 30-31 0.139349838997596 1105 32-33 0.1846609439911049 1105 34-35 0.32513311073883244 1105 36-37 0.24372805777153417 1105 38-39 0.3257946028394514 1105 40-41 0.21740484207061428 1105 42-43 0.2831855419279563 1105 44-45 0.38050245757095524 1105 46-47 0.22191656321509612 1105 48-49 0.4053558082382409 1105 50-51 0.4801818479644595 1105 52-53 0.4968800157489852 1105 54-55 0.49597244429705256 1105 56-57 0.3502323630992592 1105 58-59 0.2831185870852977 1105 60-61 0.33404442963331604 1105 62-63 0.31041654136723906 1105 64-65 0.38193883269340034 1105 66-67 0.010810662677815941 1105 68-69 -0.07219880689946123 1105 70-71 -0.05195057809401504 1105 72-73 0.15995042083253708 1105 74-75 0.23966198145872752 1105 76-77 0.22514555837614836 1105 78-79 0.22321452207968306 1105 80-81 0.13333894886796926 1105 82-83 0.26174993192693563 1105 84-85 0.4800547114321816 1105 86-87 0.69055535606417 1105 88-89 0.6430700126943556 1105 90-91 0.5427125810801847 1105 92-93 0.4725372353508739 1105 94-95 0.5292235797823182 1105 96-97 0.4726357726666066 1105 98-99 0.09011589841319534 1105 100-101 0.7499946694416302 1106 1 0.12424979875319409 1106 2 0.11531580080879422 1106 3 0.10254867761177877 1106 4 0.11087457388575217 1106 5 0.0022609762524368193 1106 6 -0.010794304431435364 1106 7 5.893995063956936E-4 1106 8 0.01603509331965114 1106 9 0.020026447847314444 1106 10-11 -0.06938958861622524 1106 12-13 -0.03218481765892278 1106 14-15 0.005462231249190097 1106 16-17 -0.1320474595697263 1106 18-19 -0.05805528168551177 1106 20-21 -0.13570463364994367 1106 22-23 -0.10877613520839446 1106 24-25 -0.1068891716875413 1106 26-27 -0.047629592545398225 1106 28-29 -0.2109901344897338 1106 30-31 -0.03770480196070736 1106 32-33 -0.07404628625862841 1106 34-35 -0.19748372537490155 1106 36-37 -0.15972372073629515 1106 38-39 -0.110280692053351 1106 40-41 -0.14928485475488174 1106 42-43 -0.15574945811779628 1106 44-45 -0.1288727998025081 1106 46-47 -0.3530546109569599 1106 48-49 -0.5553953104784171 1106 50-51 -0.2669126916001403 1106 52-53 -0.41815242474626757 1106 54-55 -0.44153499093634707 1106 56-57 -0.8231309704475933 1106 58-59 -0.7272003729886478 1106 60-61 -0.4968783629030824 1106 62-63 -0.5772073410599958 1106 64-65 -0.40014477135868987 1106 66-67 -0.49274909829658853 1106 68-69 -0.5733140919091184 1106 70-71 -0.5258551966322216 1106 72-73 -0.43157730229309266 1106 74-75 -0.509069453335421 1106 76-77 -0.6584770246010585 1106 78-79 -0.5598104969087601 1106 80-81 -0.702851420560183 1106 82-83 -0.8022422622218777 1106 84-85 -0.3629669216653575 1106 86-87 -0.2970358113361051 1106 88-89 -0.551956159327208 1106 90-91 -0.4592720542959725 1106 92-93 -0.3910674115556141 1106 94-95 -0.24146009564801219 1106 96-97 -0.3335482825325684 1106 98-99 -0.35113088727821307 1106 100-101 0.08835383925778473 1107 1 -0.026476654496839558 1107 2 -0.025385276962012426 1107 3 -0.08235566049587106 1107 4 0.09731541100174468 1107 5 -0.0493717113955654 1107 6 -0.12815246054101692 1107 7 -0.10380161189424797 1107 8 -0.14176351515136076 1107 9 -0.18272660843220478 1107 10-11 0.0363062674855712 1107 12-13 0.032275312149941726 1107 14-15 0.05663458755023498 1107 16-17 -0.11125639961671396 1107 18-19 -0.09709756346325804 1107 20-21 -0.16013883833020515 1107 22-23 -0.08008443812015997 1107 24-25 -0.03790245030022987 1107 26-27 -0.08126011823921431 1107 28-29 -0.19841690516227573 1107 30-31 -0.1691729154266497 1107 32-33 -0.1336626309987139 1107 34-35 -0.2515440310715604 1107 36-37 -0.3018103729593946 1107 38-39 -0.3981332119666874 1107 40-41 -0.2928681564070317 1107 42-43 -0.19432911834287836 1107 44-45 -0.439354842299565 1107 46-47 -0.28704343933352305 1107 48-49 -0.4140003003786106 1107 50-51 -0.5384578017819308 1107 52-53 -0.6016276744932014 1107 54-55 -0.7873354407327469 1107 56-57 -0.8802149202279637 1107 58-59 -0.6292110590884761 1107 60-61 -0.37965532893016984 1107 62-63 -0.6181258738626028 1107 64-65 -0.6243582002088672 1107 66-67 -0.3640235061639068 1107 68-69 -0.1609642721483091 1107 70-71 -0.25955981048064203 1107 72-73 -0.11198195873933514 1107 74-75 0.0057029460214899075 1107 76-77 0.04246390641336717 1107 78-79 -0.15828304421296124 1107 80-81 -0.08256809921225994 1107 82-83 -0.08512675340687537 1107 84-85 0.12872134927513557 1107 86-87 0.2851951696506063 1107 88-89 0.02473767681315664 1107 90-91 0.20602640531167182 1107 92-93 0.41475005446918445 1107 94-95 0.3225752010023335 1107 96-97 0.4396646699548228 1107 98-99 0.12947164294105207 1107 100-101 0.485738309830932 1108 1 0.07697350753338839 1108 2 0.10738712952114327 1108 3 0.03484701464385864 1108 4 0.04574166354524323 1108 5 0.13836820395488303 1108 6 0.0823920242320284 1108 7 0.15115349856101545 1108 8 0.20812939660355312 1108 9 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0.3917547899243239 2314 92-93 0.4476351281486153 2314 94-95 0.5871191854833988 2314 96-97 0.23052428133270197 2314 98-99 -0.05828618873222169 2314 100-101 0.06804443508519142 2315 1 -0.29761853445464936 2315 2 -0.27136441905416575 2315 3 -0.34296872820605273 2315 4 -0.41636986051928915 2315 5 -0.2321842211473566 2315 6 -0.1560519406536116 2315 7 -0.42895280364095356 2315 8 -0.4001609762534173 2315 9 -0.47823548022035567 2315 10-11 -0.5017522370111749 2315 12-13 -0.6794032751043986 2315 14-15 -0.7099551569996834 2315 16-17 -0.6628496080023183 2315 18-19 -0.5551344423912852 2315 20-21 -0.3620430117841167 2315 22-23 -0.418940404967195 2315 24-25 -0.43535653107313266 2315 26-27 -0.4653409847598411 2315 28-29 -0.6595915199275737 2315 30-31 -0.6037766339010702 2315 32-33 -0.8136931501995974 2315 34-35 -0.9182972936303031 2315 36-37 -0.5736044526088975 2315 38-39 -0.6506265495440005 2315 40-41 -0.7956844732575021 2315 42-43 -1.1137941585670603 2315 44-45 -1.2925935286632821 2315 46-47 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68-69 -2.1803496992929077 2316 70-71 -1.7777730542156753 2316 72-73 -1.177080116990556 2316 74-75 -1.2245342192967357 2316 76-77 -1.2080467037839817 2316 78-79 -1.6025219887958109 2316 80-81 -1.464934004497902 2316 82-83 -1.294221023754993 2316 84-85 -1.3417649776736162 2316 86-87 -1.381806252792348 2316 88-89 -1.6345993452920276 2316 90-91 -1.9815842941718245 2316 92-93 -1.711434036450914 2316 94-95 -1.7814875608619865 2316 96-97 -1.7907783540200413 2316 98-99 -2.4450669511169814 2316 100-101 -2.8626738337308595 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 3.0 4 7.0 5 21.0 6 62.0 7 143.0 8 306.0 9 651.0 10 1137.0 11 1842.0 12 2868.0 13 3984.0 14 5159.0 15 7093.0 16 9372.0 17 12496.0 18 10730.0 19 6025.0 20 5083.0 21 5294.0 22 5624.0 23 6078.0 24 7117.0 25 8363.0 26 9931.0 27 12313.0 28 15195.0 29 18787.0 30 23418.0 31 28962.0 32 36314.0 33 46139.0 34 60779.0 35 83172.0 36 115439.0 37 148906.0 38 92486.0 39 5768.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.46745906658769 19.655065101944523 14.788732394366196 20.088743437101584 2 15.498800429636916 22.54489605396561 39.20173360503518 22.754569911362292 3 21.158754018223476 26.613123812726318 30.544019151963504 21.684103017086702 4 12.411723451796519 17.3414095893831 38.50695485585313 31.739912102967242 5 12.126511987373393 39.69765898372367 33.89273154531665 14.283097483586287 6 29.135369710777866 37.60314351706063 17.58480131582084 15.67668545634067 7 27.017987150422364 35.02381851508464 20.817143810636217 17.14105052385678 8 26.4511604390586 33.02650084246157 19.818610433977486 20.703728284502347 9 26.668205638407716 17.522322408724968 21.290879459620182 34.518592493247134 10-11 24.88235021058403 27.043831838899766 26.701640635879702 21.372177314636502 12-13 27.737776656993628 24.81039719670138 27.326766709197912 20.125059437107073 14-15 23.434246868368277 25.874124996785092 28.23740995911895 22.454218175727682 16-17 20.495277071370232 29.13449149320824 28.87221808902366 21.498013346397865 18-19 20.20105262497569 27.915451254932215 31.88456254587199 19.9989335742201 20-21 21.910461954987586 25.75034281850128 31.642994521176966 20.696200705334167 22-23 21.12230143620208 25.97107522261289 31.668898807190104 21.237724533994925 24-25 21.78337132669819 26.08249724929711 32.067989095046975 20.06614232895772 26-27 21.471815360642566 25.508605726193817 33.01359439387296 20.005984519290664 28-29 20.304299768017533 27.033234091510742 32.61542978233295 20.04703635813877 30-31 20.67294935064365 26.227748653329712 32.17411269059925 20.925189305427395 32-33 20.66798327720591 26.46475013676355 32.14796561086881 20.719300975161733 34-35 21.82544835447056 25.792622452570622 31.909526775806142 20.472402417152676 36-37 21.77838333577982 25.495664698184957 32.46777850126904 20.258173464766184 38-39 21.211343730749494 26.149384548407458 32.54767224928706 20.091599471555988 40-41 21.064814379190747 26.615027441179773 32.03927893273916 20.280879246890322 42-43 20.628526798176967 27.179345627005514 30.947432475243797 21.244695099573725 44-45 21.3419392558541 26.42369091949293 30.662017845851192 21.57235197880178 46-47 22.196830502850137 27.162601865716347 29.92567119556524 20.714896435868273 48-49 22.277800987871785 27.158507377673395 29.536287413730587 21.02740422072423 50-51 21.259993664275612 27.7323438730095 30.272530243674467 20.73513221904042 52-53 21.923941149238395 28.277296638802007 28.963437201640513 20.83532501031908 54-55 20.972319281151943 27.58768864805728 29.39321475485319 22.046777315937586 56-57 21.99937384674554 27.728236939403043 28.80643770873082 21.46595150512059 58-59 23.061684734182872 27.052392868706594 28.55805407956066 21.32786831754988 60-61 21.76897553923611 27.45693138637767 30.075244582540645 20.698848491845574 62-63 21.88297575996366 27.29865009674432 29.909052809098736 20.909321334193283 64-65 21.43863730764356 28.44396926563541 29.225128091224295 20.892265335496734 66-67 21.674219555709456 27.636108831861648 28.771175063923536 21.918496548505356 68-69 21.262031656178173 28.032378830307223 29.042335370810875 21.66325414270372 70-71 22.161457892263964 27.967357887057386 28.53318127613875 21.338002944539898 72-73 21.92219243252466 27.183782081601464 29.35548867162065 21.53853681425322 74-75 21.36431243323064 28.99077665426474 28.495264119660725 21.149646792843892 76-77 21.833681182222005 27.704203607967713 28.60453313911509 21.857582070695187 78-79 21.780537107279667 27.88181029972157 28.653982004409183 21.683670588589578 80-81 22.17964089890094 28.06853752604603 28.34008757614601 21.411733998907025 82-83 21.951652593365033 27.86971749243003 28.848888002118212 21.329741912086725 84-85 21.541470724443425 28.18562207655458 28.60511727667607 21.66778992232593 86-87 21.59545975558418 28.40231303519681 28.182729027688286 21.819498181530726 88-89 21.680853587587055 28.18895058206856 27.739651016180183 22.3905448141642 90-91 21.676177588535676 27.464763980176443 28.268526840633267 22.59053159065461 92-93 21.929009885954464 28.197121689987416 28.28039363092018 21.59347479313794 94-95 21.530877089054336 27.93634816484515 28.363948532327942 22.168826213772572 96-97 20.88872011498942 27.84614515531732 28.948336356617855 22.316798373075404 98-99 21.227144049763673 28.43590918506337 27.60383647285532 22.733110292317637 100-101 20.835412731503713 28.12218888907721 27.939013527230543 23.10338485218853 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 582.0 1 449.0 2 481.5 3 628.5 4 492.5 5 403.0 6 598.5 7 1090.5 8 1643.5 9 1955.0 10 1835.0 11 1525.0 12 1471.0 13 1597.0 14 1724.5 15 1798.0 16 1879.5 17 2025.5 18 2120.0 19 2240.5 20 2402.0 21 2634.0 22 2959.5 23 3409.5 24 3941.0 25 4567.5 26 5580.0 27 6890.5 28 8001.5 29 9240.0 30 11028.0 31 12786.5 32 14917.0 33 16773.5 34 18079.5 35 19227.5 36 20914.0 37 22563.0 38 22837.0 39 22791.5 40 23121.5 41 23712.5 42 23895.0 43 24261.5 44 25310.5 45 26163.5 46 26681.5 47 32249.0 48 47926.0 49 46109.5 50 30008.0 51 23992.0 52 21093.0 53 20020.5 54 19441.0 55 18188.0 56 16399.0 57 14906.5 58 14006.5 59 12730.5 60 10670.0 61 8854.0 62 7382.0 63 5724.0 64 4081.5 65 2979.5 66 2263.0 67 1720.5 68 1221.0 69 832.0 70 648.5 71 554.5 72 466.0 73 427.5 74 353.5 75 272.0 76 205.5 77 116.5 78 77.0 79 66.5 80 46.0 81 34.5 82 28.5 83 14.5 84 7.0 85 6.5 86 4.5 87 1.5 88 1.0 89 1.0 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02070084271248788 2 0.014678779377945948 3 0.009409473960221763 4 0.0 5 0.0027601123616650502 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0010036772224236547 14-15 6.272982640147842E-5 16-17 0.0 18-19 0.0017564351392413955 20-21 0.0 22-23 1.8818947920443523E-4 24-25 0.0 26-27 0.0015682456600369604 28-29 0.0032619509728768776 30-31 0.0013173263544310468 32-33 0.009158554654615849 34-35 0.001944624618445831 36-37 0.0010036772224236547 38-39 0.0015055158336354818 40-41 6.272982640147842E-5 42-43 0.0058338738553374925 44-45 0.0026346527088620935 46-47 3.136491320073921E-4 48-49 2.509193056059137E-4 50-51 1.8818947920443523E-4 52-53 2.509193056059137E-4 54-55 0.0020073544448473094 56-57 0.01750162156601248 58-59 0.030486695631118513 60-61 0.025217390213394326 62-63 0.015431537294763691 64-65 0.022080898893320402 66-67 0.002885572014468007 68-69 6.272982640147842E-4 70-71 6.272982640147842E-5 72-73 0.0 74-75 0.001944624618445831 76-77 0.003450140452081313 78-79 0.012044126669083855 80-81 0.020387193580480484 82-83 0.02132814097650266 84-85 0.034877783479222 86-87 0.01348691267631786 88-89 0.0482392365027369 90-91 0.05532770688610396 92-93 0.03537962209043383 94-95 0.03726151688247818 96-97 0.05946787542860153 98-99 0.06323166501269024 100-101 0.07082197400726914 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 797069.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.08167065234693 #Duplication Level Percentage of deduplicated Percentage of total 1 81.38084499930238 46.45354591659873 2 11.208638116499955 12.796155788547853 3 3.1656191277783545 5.420964853878414 4 1.310752195388911 2.99279700496122 5 0.7023156850109555 2.0044676312886396 6 0.4495307348510573 1.5395979212925328 7 0.29880143611358745 1.1939259616678857 8 0.2095331324534834 0.956840100597146 9 0.15637109727829981 0.8033331126947412 >10 0.9940427814220973 10.530437334230026 >50 0.07527190197489922 3.0210480427190265 >100 0.040159176924014356 4.2170524986519915 >500 0.0037306339198577053 1.4688595747454962 >1k 0.0037306339198577053 4.144523954462689 >5k 6.583471623278303E-4 2.456450303663584 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8236 1.0332857004851526 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5862 0.7354444847309329 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5512 0.6915336062498981 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3400 0.4265628195300532 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3330 0.4177806438338463 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2678 0.3359809502063184 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2547 0.319545735689131 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2509 0.31477826888261873 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2493 0.3127709144377714 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2025 0.2540557969259876 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1849 0.2319748980326672 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1718 0.21553968351547986 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1646 0.20650658851366693 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1620 0.20324463754079009 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1434 0.1799091421194401 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1417 0.17777632802178983 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1295 0.1624702503798291 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1065 0.13361453023514905 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1034 0.12972528099825736 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1026 0.12872160377583372 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 976 0.12244862113568587 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 893 0.11203546995304045 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 854 0.10714254349372514 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 812 0.10187323807600095 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 803 0.10074410120077433 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 1.2545965280295684E-4 0.0 0.0 0.0 0.0 7 1.2545965280295684E-4 0.0 0.0 0.0 0.0 8 1.2545965280295684E-4 0.0 0.0 0.0 0.0 9 1.2545965280295684E-4 0.0 0.0 0.0 0.0 10-11 1.2545965280295684E-4 0.0 0.0 0.0 1.2545965280295684E-4 12-13 1.2545965280295684E-4 0.0 0.0 0.0 2.509193056059137E-4 14-15 1.8818947920443526E-4 0.0 0.0 0.0 2.509193056059137E-4 16-17 2.509193056059137E-4 0.0 0.0 1.2545965280295684E-4 2.509193056059137E-4 18-19 3.136491320073921E-4 0.0 0.0 1.2545965280295684E-4 2.509193056059137E-4 20-21 3.763789584088705E-4 0.0 0.0 3.136491320073921E-4 2.509193056059137E-4 22-23 3.763789584088705E-4 0.0 0.0 5.018386112118274E-4 2.509193056059137E-4 24-25 3.763789584088705E-4 0.0 0.0 0.0013173263544310468 2.509193056059137E-4 26-27 3.763789584088705E-4 0.0 0.0 0.0019446246184458308 2.509193056059137E-4 28-29 5.645684376133057E-4 0.0 0.0 0.0033874106256798343 2.509193056059137E-4 30-31 7.52757916817741E-4 0.0 0.0 0.007276659862571496 2.509193056059137E-4 32-33 7.52757916817741E-4 0.0 0.0 0.013549642502719339 2.509193056059137E-4 34-35 7.52757916817741E-4 0.0 0.0 0.019195326878852396 3.763789584088705E-4 36-37 7.52757916817741E-4 0.0 0.0 0.02540557969259876 3.763789584088705E-4 38-39 0.0010036772224236547 0.0 0.0 0.031427643027140684 5.018386112118274E-4 40-41 0.0010036772224236547 0.0 0.0 0.041966253862589065 5.018386112118274E-4 42-43 0.001066407048825133 0.0 0.0 0.05576681567091431 5.018386112118274E-4 44-45 0.0013800561808325251 0.0 0.0 0.06950464765283809 5.018386112118274E-4 46-47 0.0013800561808325251 0.0 0.0 0.08612805164922988 5.018386112118274E-4 48-49 0.0013800561808325251 0.0 0.0 0.09879947658232852 5.018386112118274E-4 50-51 0.0013800561808325251 0.0 0.0 0.1176811543291735 6.272982640147842E-4 52-53 0.0013800561808325251 0.0 0.0 0.15945921871255814 6.272982640147842E-4 54-55 0.0013800561808325251 0.0 0.0 0.21058402722976305 6.272982640147842E-4 56-57 0.0013800561808325251 0.0 0.0 0.254243986405192 6.900280904162627E-4 58-59 0.0013800561808325251 0.0 0.0 0.3228704164884094 7.52757916817741E-4 60-61 0.0013800561808325251 0.0 0.0 0.40435646098392986 7.52757916817741E-4 62-63 0.0013800561808325251 0.0 0.0 0.4561085677651495 7.52757916817741E-4 64-65 0.0013800561808325251 0.0 0.0 0.49600473735648987 7.52757916817741E-4 66-67 0.0013800561808325251 0.0 0.0 0.6155050566513063 7.52757916817741E-4 68-69 0.001505515833635482 0.0 0.0 0.736510891779758 7.52757916817741E-4 70-71 0.0015682456600369604 0.0 0.0 0.7888275669985911 7.52757916817741E-4 72-73 0.0016309754864384388 0.0 0.0 0.887815233060124 7.52757916817741E-4 74-75 0.0016309754864384388 0.0 0.0 1.0289573424634506 7.52757916817741E-4 76-77 0.0016309754864384388 0.0 0.0 1.174302350235676 7.52757916817741E-4 78-79 0.0016309754864384388 0.0 0.0 1.3006402206082535 7.52757916817741E-4 80-81 0.0020073544448473094 0.0 0.0 1.4607267375848263 7.52757916817741E-4 82-83 0.0020073544448473094 0.0 0.0 1.634927465501732 7.52757916817741E-4 84-85 0.0020073544448473094 0.0 0.0 1.8270061939430589 7.52757916817741E-4 86-87 0.0020073544448473094 0.0 0.0 2.1629244143229758 8.154877432192194E-4 88-89 0.0020073544448473094 0.0 0.0 2.5701037174949724 8.782175696206979E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 745 0.0 37.623936 2 GTACCTG 915 0.0 30.64331 1 TAACGCG 45 8.943344E-4 26.391785 76-77 CGTAGCT 120 1.712543E-4 23.745155 8 CTAGTAC 190 3.294772E-7 22.492588 3 TATAGGG 170 3.1422678E-6 22.356796 2 GTACATG 5670 0.0 22.043344 1 GTGTAGC 345 0.0 22.039701 1 GAGTACT 5380 0.0 21.981092 12-13 TACATGG 5635 0.0 21.920372 2 TCTATAC 220 5.653419E-8 21.583794 3 ACCTGGG 1245 0.0 21.358421 3 ACATGGG 5650 0.0 21.262903 3 GTATAGG 250 9.424184E-9 20.910166 1 CATGGGA 3930 0.0 20.661129 4 GTACTTT 5760 0.0 20.40471 14-15 CATGGGT 550 0.0 19.85709 4 AGTACTT 5535 0.0 19.477825 12-13 ATGGGTA 275 2.8447175E-8 18.993738 5 ACTTTTT 6405 0.0 18.609398 16-17 >>END_MODULE