FastQCFastQC Report
Thu 26 May 2016
SRR936519_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936519_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1627659
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85390.5246184858130604No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63790.39191255662273244No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40480.24870074137150347No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT25800.15850985986622504No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA135450.028.7251971
GTACATG114350.027.0459921
TACATGG116100.026.2299372
GGTATCA95850.025.525441
ACATGGG115850.025.179313
GAGTACT71000.024.15499512-13
ATCAACG162700.023.7688563
TCAACGC163250.023.7193374
CAACGCA164000.023.5232325
TATCAAC166050.023.2321072
CATGGGG40150.023.1936284
AACGCAG166950.023.0805386
GTACTTT76650.022.28357714-15
AGTACTT73500.021.4589712-13
CATGGGA71100.021.3834884
ACGCAGA182400.021.0454397
CGCAGAG183750.020.8649648
TAAGGTG5500.020.7316275
ATGGGAG26700.020.6410375
GCAGAGT187800.019.9596489
Produced by FastQC (version 0.11.5)