##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936519_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1627659 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.495795495248082 31.0 31.0 34.0 30.0 34.0 2 31.567019258947973 33.0 31.0 34.0 30.0 34.0 3 31.571174306166096 33.0 31.0 34.0 28.0 34.0 4 35.21369525189244 37.0 35.0 37.0 33.0 37.0 5 35.235802462309366 37.0 35.0 37.0 33.0 37.0 6 35.23411169047079 37.0 35.0 37.0 32.0 37.0 7 35.1430655929774 37.0 35.0 37.0 32.0 37.0 8 35.095513863776134 37.0 35.0 37.0 32.0 37.0 9 36.60255311462659 39.0 37.0 39.0 32.0 39.0 10-11 36.47088026423225 39.0 37.0 39.0 32.0 39.0 12-13 36.3742565242474 39.0 37.0 39.0 32.0 39.0 14-15 37.46238800633302 40.0 37.0 41.0 32.0 41.0 16-17 37.365024246479145 40.0 37.0 41.0 32.0 41.0 18-19 37.3491628160444 40.0 37.0 41.0 32.0 41.0 20-21 37.229424283587655 39.5 36.5 41.0 31.0 41.0 22-23 37.32984857393349 40.0 37.0 41.0 32.0 41.0 24-25 37.37175231421324 40.0 37.0 41.0 32.0 41.0 26-27 37.27531718867404 40.0 37.0 41.0 31.0 41.0 28-29 37.12406069084495 39.0 36.0 41.0 31.0 41.0 30-31 36.875118498407836 39.0 36.0 41.0 30.5 41.0 32-33 36.67334681281521 39.0 35.5 41.0 30.0 41.0 34-35 36.47819598576852 39.0 35.0 41.0 30.0 41.0 36-37 36.25927789543142 39.0 35.0 40.0 30.0 41.0 38-39 36.031885057005184 38.0 35.0 40.0 29.5 41.0 40-41 35.788618193368514 38.0 35.0 40.0 28.5 41.0 42-43 35.49894265322159 38.0 35.0 40.0 27.0 41.0 44-45 35.18061707028315 38.0 34.0 40.0 26.5 41.0 46-47 34.881614637955494 38.0 34.0 40.0 24.5 41.0 48-49 34.6574651078635 38.0 34.0 40.0 24.0 41.0 50-51 33.66227907688281 37.5 33.0 39.5 23.0 40.5 52-53 33.73800531929599 37.5 33.0 39.5 23.0 40.5 54-55 34.144587410507974 38.0 34.0 40.0 23.0 41.0 56-57 34.032253377396614 38.0 33.5 40.0 22.5 41.0 58-59 33.791868567064725 37.5 33.0 40.0 22.0 41.0 60-61 33.484292471580346 37.0 33.0 40.0 21.5 41.0 62-63 33.10936350918712 36.5 32.5 39.5 20.0 41.0 64-65 32.74986437576912 36.0 32.0 39.0 18.5 41.0 66-67 32.298017889496506 35.0 31.5 39.0 17.5 40.5 68-69 31.912242060529877 35.0 31.0 38.5 13.5 40.0 70-71 31.527073545503082 35.0 31.0 38.0 12.0 40.0 72-73 31.147607391966005 35.0 31.0 37.0 8.0 39.5 74-75 30.717236534188057 35.0 30.5 36.5 7.0 39.0 76-77 30.267941872345496 34.5 30.0 36.0 4.0 39.0 78-79 29.78258990365918 34.0 29.0 35.5 2.0 37.5 80-81 29.336548994598992 34.0 29.0 35.0 2.0 37.0 82-83 28.934466003014144 34.0 29.0 35.0 2.0 37.0 84-85 28.575313993901673 34.0 29.0 35.0 2.0 36.0 86-87 28.207600302028865 34.0 27.5 35.0 2.0 36.0 88-89 27.806864951442535 34.0 27.0 35.0 2.0 35.5 90-91 27.40072121986239 33.0 26.0 35.0 2.0 35.0 92-93 26.956535429104008 33.0 25.5 35.0 2.0 35.0 94-95 26.45622301722904 33.0 25.0 35.0 2.0 35.0 96-97 25.85034058116596 33.0 23.5 35.0 2.0 35.0 98-99 25.06850329215149 32.0 18.0 35.0 2.0 35.0 100-101 22.11050226122302 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.44224970646254036 1101 2 0.4881950970597728 1101 3 0.4753076145879973 1101 4 0.47219625398198417 1101 5 0.4143751335341861 1101 6 0.5776086566429441 1101 7 0.5738075336660273 1101 8 0.5303393005809554 1101 9 0.6051909176592929 1101 10-11 0.7502956605318829 1101 12-13 0.6572124905327641 1101 14-15 0.7629974161572122 1101 16-17 0.902576466796603 1101 18-19 0.7723016965262133 1101 20-21 0.682979179354831 1101 22-23 0.8358185490903409 1101 24-25 0.8954562615558999 1101 26-27 0.8411208513232893 1101 28-29 0.9545562172223256 1101 30-31 1.0159120974905633 1101 32-33 0.9208463795726587 1101 34-35 0.7235905393910897 1101 36-37 0.6109452772515169 1101 38-39 0.7980373360211459 1101 40-41 0.7016733009114731 1101 42-43 0.9239016080890252 1101 44-45 0.6718384439003842 1101 46-47 1.0006916806845396 1101 48-49 1.1566997198558369 1101 50-51 1.0713744323736378 1101 52-53 1.2675660535074158 1101 54-55 1.2367188697199438 1101 56-57 1.1098011057930819 1101 58-59 1.2024268551176505 1101 60-61 1.307963204814989 1101 62-63 1.1956604346236617 1101 64-65 1.202055699791707 1101 66-67 1.2565648827038913 1101 68-69 1.2208113279266293 1101 70-71 1.118100427876243 1101 72-73 0.919969866300864 1101 74-75 0.9276318952388536 1101 76-77 0.9558244550223378 1101 78-79 0.8012492642540749 1101 80-81 0.934090627445741 1101 82-83 0.7834628170623681 1101 84-85 0.890191370061924 1101 86-87 0.9004893351040408 1101 88-89 0.7957702060411087 1101 90-91 0.9300797149464053 1101 92-93 1.0043103129374664 1101 94-95 0.9547157068215277 1101 96-97 0.7149356023730746 1101 98-99 0.7749201812938722 1101 100-101 0.9448124976736416 1102 1 0.309395976663712 1102 2 0.34299316139918545 1102 3 0.41900226108646166 1102 4 0.3927542781473079 1102 5 0.4144398077284208 1102 6 0.4816755221510505 1102 7 0.4262872183603079 1102 8 0.4042288066222852 1102 9 0.5420445561533711 1102 10-11 0.595119822810851 1102 12-13 0.5851477478599705 1102 14-15 0.6293133838353384 1102 16-17 0.5512186891540907 1102 18-19 0.5998529032705946 1102 20-21 0.5323330080671269 1102 22-23 0.4115541229406574 1102 24-25 0.601875175333781 1102 26-27 0.4408090836634315 1102 28-29 0.37184800436052967 1102 30-31 0.5917713131828108 1102 32-33 0.4702703988075214 1102 34-35 0.515814038188914 1102 36-37 0.2471293476170402 1102 38-39 0.23848457709610926 1102 40-41 0.3722857274872311 1102 42-43 0.42021574967225206 1102 44-45 0.49773418149055715 1102 46-47 0.5344088109764087 1102 48-49 0.5316816491250975 1102 50-51 0.751394861810283 1102 52-53 0.814169326782519 1102 54-55 0.7518902943783061 1102 56-57 0.7707713708811852 1102 58-59 0.8278027263170884 1102 60-61 0.6484888157959077 1102 62-63 0.8610346319027826 1102 64-65 0.872026330098798 1102 66-67 0.9726917734729881 1102 68-69 0.9689850428123457 1102 70-71 0.9497801308314777 1102 72-73 0.6718583527725421 1102 74-75 0.7318132721144046 1102 76-77 0.8115003764393869 1102 78-79 0.7858450124964591 1102 80-81 0.7673318317553246 1102 82-83 0.721663961415171 1102 84-85 0.532198020090835 1102 86-87 0.4814273772259625 1102 88-89 0.56238690872242 1102 90-91 0.6362028393947732 1102 92-93 0.6853655294818886 1102 94-95 0.37087759774279405 1102 96-97 0.3777551689798848 1102 98-99 0.45933978746607806 1102 100-101 0.5806498002271319 1103 1 0.4084665426120928 1103 2 0.3821300309232214 1103 3 0.4884966963220734 1103 4 0.48774599020035225 1103 5 0.48341328629815905 1103 6 0.5046864148813412 1103 7 0.5235877156371274 1103 8 0.6700499053672502 1103 9 0.48482812233854844 1103 10-11 0.7835522168435247 1103 12-13 0.3471075513172224 1103 14-15 0.6429041368545114 1103 16-17 0.6279769179014281 1103 18-19 0.7432255056859347 1103 20-21 0.823235506291141 1103 22-23 0.5749643171154091 1103 24-25 0.8002368258192192 1103 26-27 0.704977032988559 1103 28-29 0.7407430434322819 1103 30-31 0.802304405771018 1103 32-33 0.30852223399577383 1103 34-35 0.6560490606847722 1103 36-37 0.871942868810514 1103 38-39 0.8243151477804531 1103 40-41 0.6942310270373184 1103 42-43 0.5858281697527588 1103 44-45 0.7800290548033644 1103 46-47 0.8154634522267088 1103 48-49 0.7186148127528469 1103 50-51 0.7860754798543255 1103 52-53 0.8614121044287444 1103 54-55 1.029293798995873 1103 56-57 0.8254540609119729 1103 58-59 0.7946538984695906 1103 60-61 0.965112069407084 1103 62-63 0.879561153333043 1103 64-65 0.8766705403758479 1103 66-67 0.999356762817051 1103 68-69 0.9053380501531016 1103 70-71 0.7039537041206074 1103 72-73 0.5871644148137456 1103 74-75 0.5800432034428447 1103 76-77 0.6621915320159921 1103 78-79 0.5956247899058837 1103 80-81 0.6840868045234991 1103 82-83 0.5962791638778775 1103 84-85 0.7055700305099677 1103 86-87 0.7336044432927409 1103 88-89 0.6632897029445424 1103 90-91 0.6620641938821059 1103 92-93 0.5955730241539179 1103 94-95 0.7691090090617578 1103 96-97 1.0648170917835778 1103 98-99 0.8500128871582362 1103 100-101 0.9599550926732618 1104 1 0.4097908162903998 1104 2 0.4963565665642591 1104 3 0.37787594764634846 1104 4 0.470799344781085 1104 5 0.46937155876654657 1104 6 0.4831357973787931 1104 7 0.6241952584639137 1104 8 0.5896694441216255 1104 9 0.5252701125116275 1104 10-11 0.7555368198565304 1104 12-13 0.6857171377306983 1104 14-15 0.4898741691682673 1104 16-17 0.6101329756235359 1104 18-19 0.5900105454510367 1104 20-21 0.38877580257432953 1104 22-23 0.49670509146508124 1104 24-25 0.60587808413743 1104 26-27 0.36423034920593267 1104 28-29 0.4175271241133771 1104 30-31 0.5434598068894516 1104 32-33 0.6047104284094829 1104 34-35 0.5412278929631142 1104 36-37 0.32660825915770175 1104 38-39 0.21252339442735035 1104 40-41 0.14546695432400014 1104 42-43 0.26337804212082006 1104 44-45 0.22585593276360783 1104 46-47 0.22155732441769516 1104 48-49 0.452140516204075 1104 50-51 0.49089266368859796 1104 52-53 0.37024987905875406 1104 54-55 0.28779404983323076 1104 56-57 0.4107140464519361 1104 58-59 0.4359636752243432 1104 60-61 0.4359728841858299 1104 62-63 0.5779570853413674 1104 64-65 0.5437154919052247 1104 66-67 0.4969542573945347 1104 68-69 0.3096085561067987 1104 70-71 0.25473904636604416 1104 72-73 0.26071121811299136 1104 74-75 0.2307556921920515 1104 76-77 0.15608843786345616 1104 78-79 0.1776557978293951 1104 80-81 0.2463933277702175 1104 82-83 0.24171503066848743 1104 84-85 0.16921023383577705 1104 86-87 0.1057579926412977 1104 88-89 0.019517662456440377 1104 90-91 0.119712888790108 1104 92-93 0.09173263048682756 1104 94-95 0.2951820765074977 1104 96-97 0.3540930571385168 1104 98-99 0.3824326929806148 1104 100-101 0.48205607684100116 1105 1 0.3147447720610508 1105 2 0.13788592450366366 1105 3 0.3581269087532313 1105 4 0.25622152710625556 1105 5 0.17272616329789514 1105 6 0.320595439562986 1105 7 0.364840593219661 1105 8 0.46705649102450764 1105 9 0.5152904296497951 1105 10-11 0.46446291707956533 1105 12-13 0.3197679674375138 1105 14-15 0.441103280136268 1105 16-17 0.383658140036232 1105 18-19 0.38671014886570987 1105 20-21 0.294722305836558 1105 22-23 0.17530780576534966 1105 24-25 0.3298365868705915 1105 26-27 0.20416621036419969 1105 28-29 0.25478673897676174 1105 30-31 0.08802795386670681 1105 32-33 0.06285864160715704 1105 34-35 0.1365113679960146 1105 36-37 0.09024101550165398 1105 38-39 -0.04428241319515536 1105 40-41 0.015151052097785112 1105 42-43 0.022835702462501217 1105 44-45 0.11496436383387021 1105 46-47 0.174703770436345 1105 48-49 0.1878323288527426 1105 50-51 -0.024275803766037996 1105 52-53 -0.01620940968962259 1105 54-55 0.07286659090659242 1105 56-57 0.05170999820252575 1105 58-59 0.1625149440155269 1105 60-61 -0.03645858070802177 1105 62-63 0.15516763117202004 1105 64-65 0.0678873571139107 1105 66-67 0.15205294838105488 1105 68-69 0.20187839458879964 1105 70-71 0.007728072509451067 1105 72-73 -0.07393394751396443 1105 74-75 0.2388679113135801 1105 76-77 0.11672208538604067 1105 78-79 0.21281561369584523 1105 80-81 0.41835990531902567 1105 82-83 0.31787123194909483 1105 84-85 0.3218050597957465 1105 86-87 0.190146754683596 1105 88-89 0.3959072831525994 1105 90-91 0.28414727223976755 1105 92-93 0.015394832587059426 1105 94-95 0.24665512323257843 1105 96-97 0.2658734195511592 1105 98-99 0.4619308141332077 1105 100-101 0.3464370112558903 1106 1 0.23996565028609496 1106 2 0.151062214147494 1106 3 0.2804657623542006 1106 4 0.3215205291652552 1106 5 0.1680950233594274 1106 6 0.31827951422696543 1106 7 0.5013241820261811 1106 8 0.4647405656884871 1106 9 0.5475140235878087 1106 10-11 0.5732979039345523 1106 12-13 0.5417759034885492 1106 14-15 0.5042366770091888 1106 16-17 0.41799030279137384 1106 18-19 0.41009790306844707 1106 20-21 0.2500127222340325 1106 22-23 0.46645003989947753 1106 24-25 0.3501029769197572 1106 26-27 0.3987753673133625 1106 28-29 0.3510568447410236 1106 30-31 0.5447345069012925 1106 32-33 0.4250079567798295 1106 34-35 0.2701626835283193 1106 36-37 0.4994785618503599 1106 38-39 0.35399242321174285 1106 40-41 0.2689856373882975 1106 42-43 0.2543216260287906 1106 44-45 0.27076373654042385 1106 46-47 0.6872064623397378 1106 48-49 0.6694104700996704 1106 50-51 0.3873693525491504 1106 52-53 0.6270947338160155 1106 54-55 0.7077515622320405 1106 56-57 0.6017925499066124 1106 58-59 0.7185310549240782 1106 60-61 0.6248681669680565 1106 62-63 0.6343722776638003 1106 64-65 0.6932008794597309 1106 66-67 0.6650170840544121 1106 68-69 0.80576934105137 1106 70-71 0.6039388320206669 1106 72-73 0.33144609238661715 1106 74-75 0.5355979327706244 1106 76-77 0.4774056902551358 1106 78-79 0.38297606890647984 1106 80-81 0.477305126950629 1106 82-83 0.5151518587023851 1106 84-85 0.7469962648942641 1106 86-87 0.6489003980815546 1106 88-89 0.645792677364529 1106 90-91 0.47992558591066015 1106 92-93 0.38012091231572853 1106 94-95 0.5197633814591391 1106 96-97 0.39717878125413364 1106 98-99 0.6750798116079721 1106 100-101 0.15683195168575637 1107 1 0.2056730004670797 1107 2 0.24718476095542385 1107 3 0.1745672532976279 1107 4 0.21363625576375256 1107 5 0.2556345458109277 1107 6 0.3636136196063191 1107 7 0.4230689355825419 1107 8 0.41830439279667075 1107 9 0.47693450654626446 1107 10-11 0.4576350555848805 1107 12-13 0.36666216582429456 1107 14-15 0.1600755039500683 1107 16-17 0.3325446733924835 1107 18-19 0.3324702962244288 1107 20-21 0.3862936370944823 1107 22-23 0.4155726652504015 1107 24-25 0.38278567128991625 1107 26-27 0.46011393027227143 1107 28-29 0.3630038342018196 1107 30-31 0.36125435450673393 1107 32-33 0.1050725451916037 1107 34-35 0.30330642180189926 1107 36-37 0.23025833184968292 1107 38-39 0.1419224417485978 1107 40-41 0.28280216163853567 1107 42-43 0.2651107225694176 1107 44-45 0.1836424643832828 1107 46-47 0.13797496863849545 1107 48-49 0.21075314688041402 1107 50-51 0.0833646103832919 1107 52-53 0.3189351372523177 1107 54-55 0.24618339306943682 1107 56-57 0.12883353209567616 1107 58-59 0.15147258899219196 1107 60-61 0.24856670413104354 1107 62-63 0.19708974580369443 1107 64-65 0.016951085943297528 1107 66-67 0.0482206453279872 1107 68-69 0.306175968390189 1107 70-71 0.0970839298278392 1107 72-73 0.08551439098668467 1107 74-75 0.14392986606613434 1107 76-77 0.2293531516783709 1107 78-79 -0.07813457846044258 1107 80-81 0.05240403654128656 1107 82-83 0.08843970049212402 1107 84-85 -0.1420200068912969 1107 86-87 -0.16513759630349867 1107 88-89 -0.29893664689444677 1107 90-91 -0.31057909338558076 1107 92-93 0.004870270323539927 1107 94-95 0.2718115751637953 1107 96-97 0.09082179943278845 1107 98-99 0.2802131427575958 1107 100-101 0.4669739665920183 1108 1 0.43117241804237594 1108 2 0.3984934131939575 1108 3 0.42807126456474265 1108 4 0.44885620232780354 1108 5 0.488386954249556 1108 6 0.48157158361239993 1108 7 0.5012473996525415 1108 8 0.50680223700396 1108 9 0.5924327885540919 1108 10-11 0.6728270557620064 1108 12-13 0.44340010184827605 1108 14-15 0.6133386892096837 1108 16-17 0.679849950353109 1108 18-19 0.7801672368342523 1108 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-5.194088648187972 2316 100-101 -5.824162536607123 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3073.0 3 1282.0 4 1309.0 5 1472.0 6 1700.0 7 2058.0 8 2650.0 9 3212.0 10 4156.0 11 5405.0 12 6921.0 13 8799.0 14 9994.0 15 12669.0 16 15661.0 17 20295.0 18 15725.0 19 11399.0 20 11266.0 21 12119.0 22 13023.0 23 14699.0 24 17141.0 25 19533.0 26 22700.0 27 26597.0 28 31601.0 29 38015.0 30 46377.0 31 57373.0 32 71586.0 33 92002.0 34 121689.0 35 167002.0 36 226574.0 37 280785.0 38 204263.0 39 25528.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.00688188957025 18.55080923647893 14.395991299325328 22.04631757462549 2 15.713104217819495 20.483497145272803 39.742337214189334 24.061061422718367 3 19.2815806969745 25.729584118311404 29.407275502416685 25.581559682297407 4 12.534219344229857 16.592856322075693 37.072968699784745 33.799955633909704 5 13.753294808629157 38.06958168759345 32.79727332116095 15.379850182616439 6 31.304559485744 35.82986967804909 17.72537116739013 15.140199668816775 7 28.939482843897746 33.61864883214392 19.92633121461333 17.515537109345 8 27.54645277536743 32.872759094518095 19.62391626578436 19.956871864330118 9 27.938090854001057 16.378279312688758 20.03375342789046 35.649876405419725 10-11 25.551121757689277 26.433561920624943 26.334915466749848 21.680400854935932 12-13 26.998940199797993 24.044866206754143 27.052959589624713 21.903234003823147 14-15 23.661539397541013 25.103420600049965 26.36554676719019 24.869493235218837 16-17 22.353432819310502 28.782461870793192 26.460463465559652 22.403641844336658 18-19 22.216582272258 26.496054766235684 29.70754879307802 21.579814168428296 20-21 23.503948285633847 25.36366274030571 29.497037710031215 21.635351264029232 22-23 22.738659810917994 25.585827329023093 29.543971459505165 22.131541400553754 24-25 22.589116919358396 25.846195247138592 29.62409890827588 21.94058892522713 26-27 22.330094999654406 26.0662463232753 29.620661848845337 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23.14700041011368 26.9745744878571 27.283219592067447 22.59520550996177 54-55 22.576122268730078 26.782012979532276 27.472646979762683 23.169217771974964 56-57 22.775066292137595 27.22158530980976 27.044150932267748 22.9591974657849 58-59 23.100743567971158 26.610142311364633 27.15642084644603 23.132693274218184 60-61 22.859532861640243 26.7366854016805 27.793202556874736 22.610579179804525 62-63 22.838150550040965 26.809025150654183 27.650697953462394 22.702126345842462 64-65 22.59012253900574 27.00604247240645 27.448583689932388 22.95525129865542 66-67 22.631778494270822 27.18436668712912 27.113884910078895 23.069969908521166 68-69 22.69320920101034 26.920629008401182 27.52918225158554 22.85697953900294 70-71 22.69555555897056 26.95211326478394 27.312727535372193 23.039603640873306 72-73 22.744201279565058 26.97040975084612 27.29930294919345 22.986086020395373 74-75 22.482017207064615 27.51885854089328 27.01937492490845 22.979749327133653 76-77 22.64725366974225 27.16681641025893 27.15474064450789 23.031189275490927 78-79 22.720202070186687 27.25863625368562 27.183364002651896 22.837797673475798 80-81 22.568777160003258 27.753153903738877 26.908474920182062 22.7695940160758 82-83 22.729601487992884 27.599077872221805 26.719854052779862 22.95146658700545 84-85 22.563210780435575 27.39391388371275 26.700964887081636 23.341910448770044 86-87 22.73839930414413 27.280020229225638 26.758957899921253 23.222622566708974 88-89 22.70857356705482 27.311321088057895 26.528531700711877 23.451573644175415 90-91 22.570086593735923 27.28778265580757 26.874082296316647 23.268048454139862 92-93 22.823263789987884 27.13179866628443 26.944858484377253 23.100079059350435 94-95 22.49456050542905 26.91494480011355 27.068098600939262 23.52239609351814 96-97 22.46617708417185 26.946373081033055 27.07701307567472 23.510436759120374 98-99 22.308427796974602 27.125159163923396 26.70029175826692 23.86612128083508 100-101 22.436249563879542 27.049643188144284 26.830069026148784 23.684038221827386 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 677.0 1 485.5 2 452.0 3 605.0 4 569.5 5 564.0 6 843.5 7 1656.5 8 2617.0 9 2938.5 10 2548.5 11 2147.0 12 2184.5 13 2380.0 14 2439.5 15 2507.5 16 2595.5 17 2690.0 18 2785.5 19 2836.0 20 3176.0 21 3637.5 22 4160.0 23 4943.0 24 5697.5 25 6656.0 26 8168.5 27 9947.5 28 11787.5 29 14050.5 30 16940.0 31 20048.0 32 24078.5 33 27412.5 34 29841.0 35 32000.0 36 35050.5 37 38497.0 38 40358.5 39 41349.0 40 42113.5 41 45465.5 42 48932.5 43 51487.5 44 53735.5 45 55917.5 46 56900.0 47 58844.0 48 68035.0 49 69269.0 50 61780.0 51 56817.0 52 53163.5 53 53195.0 54 53993.5 55 51816.5 56 47364.5 57 42493.0 58 39846.0 59 36618.0 60 31696.0 61 27491.0 62 23663.5 63 18656.5 64 14110.0 65 11547.0 66 8780.5 67 6910.5 68 5194.0 69 3444.0 70 2716.0 71 2297.5 72 1979.0 73 1923.0 74 1583.5 75 1281.5 76 973.5 77 580.5 78 359.0 79 232.0 80 149.0 81 104.0 82 69.5 83 45.5 84 29.5 85 21.0 86 14.0 87 7.5 88 8.0 89 7.0 90 3.0 91 3.5 92 4.0 93 2.5 94 0.5 95 1.0 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01222614810596077 2 0.01124314122306945 3 0.013823534290659162 4 0.01781699975240514 5 0.02936733062637813 6 0.04700001658824115 7 0.0063895447387935685 8 0.025496741024993563 9 0.034712430552099674 10-11 0.03910524256002025 12-13 0.02859935649911929 14-15 0.02730915996532443 16-17 0.02835360477839646 18-19 0.023284975538488097 20-21 0.05388106476848037 22-23 0.013332030849213503 24-25 0.0030718965090353696 26-27 0.0013209154988852087 28-29 9.215689527106108E-5 30-31 0.002611112032680064 32-33 0.002181046521415112 34-35 3.071896509035369E-5 36-37 3.379086159938906E-4 38-39 3.071896509035369E-5 40-41 0.0016588241148790994 42-43 0.004239217182468809 44-45 0.0022117654865054658 46-47 0.001290196533794855 48-49 4.91503441445659E-4 50-51 0.004024184426836334 52-53 0.003778432706113504 54-55 0.005928760262438262 56-57 9.215689527106108E-4 58-59 0.006205230948251445 60-61 0.0028875827184932468 62-63 4.607844763553054E-4 64-65 8.601310225299034E-4 66-67 0.017694123892043726 68-69 0.04211570113887491 70-71 0.052314397548872335 72-73 0.014192161871743407 74-75 0.02939804959146848 76-77 0.026602623768246295 78-79 0.05514054233718488 80-81 0.07996146613019066 82-83 0.047276487274054334 84-85 0.01637320839315852 86-87 0.01907647732110964 88-89 0.020735301435988743 90-91 0.0390745235949299 92-93 0.024605891037373307 94-95 0.012901965337948552 96-97 0.01757124803168231 98-99 0.049058187249294845 100-101 0.06767388009404919 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1627659.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.81397639991781 #Duplication Level Percentage of deduplicated Percentage of total 1 73.66412218318294 35.22174599587372 2 14.232830193916874 13.610564139919582 3 5.061058056094666 7.259679313581728 4 2.312557924849814 4.422903601688477 5 1.2553155814960715 3.001081479405114 6 0.743074933154618 2.131762039033525 7 0.4881016797279015 1.6336657536689088 8 0.3356977552359989 1.2840835637087549 9 0.2532784777690974 1.0899226042792858 >10 1.397602217310089 12.598729662739498 >50 0.14934969740048307 4.979412078774741 >100 0.09922881807452172 8.674402714607874 >500 0.006225985429640409 2.0716682037104492 >1k 0.0012970802978417518 1.095185870234787 >5k 2.5941605956835036E-4 0.9251929787735745 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8539 0.5246184858130604 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6379 0.39191255662273244 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4048 0.24870074137150347 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2580 0.15850985986622504 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 3.0718965090353695E-5 0.0 18-19 0.0 0.0 0.0 6.143793018070739E-5 0.0 20-21 0.0 0.0 0.0 2.1503275563247585E-4 0.0 22-23 0.0 0.0 0.0 3.9934654617459803E-4 0.0 24-25 0.0 0.0 0.0 7.98693092349196E-4 0.0 26-27 0.0 0.0 0.0 0.0010751637781623792 0.0 28-29 0.0 0.0 0.0 0.0020581706610536977 0.0 30-31 0.0 0.0 0.0 0.003870589601384565 0.0 32-33 0.0 0.0 0.0 0.006266668878432153 0.0 34-35 0.0 0.0 0.0 0.009830068828913182 0.0 36-37 0.0 0.0 0.0 0.013792815325568808 0.0 38-39 0.0 0.0 0.0 0.019967327308729903 0.0 40-41 0.0 0.0 0.0 0.027186284104963016 0.0 42-43 0.0 0.0 0.0 0.03498890123791286 0.0 44-45 0.0 0.0 0.0 0.03996537358255015 0.0 46-47 0.0 0.0 0.0 0.0482287751918553 0.0 48-49 0.0 0.0 0.0 0.05593923542953408 0.0 50-51 0.0 0.0 0.0 0.06288172153995401 0.0 52-53 0.0 0.0 0.0 0.07685885065606493 0.0 54-55 0.0 0.0 0.0 0.09870003483530641 0.0 56-57 3.0718965090353695E-5 0.0 0.0 0.1330131188412315 0.0 58-59 6.143793018070739E-5 0.0 0.0 0.17568176135173275 0.0 60-61 6.143793018070739E-5 0.0 0.0 0.2281497537260569 0.0 62-63 6.143793018070739E-5 0.0 0.0 0.2683301600642395 0.0 64-65 6.143793018070739E-5 0.0 0.0 0.31440860769977 0.0 66-67 6.143793018070739E-5 0.0 0.0 0.39624393070047226 0.0 68-69 6.143793018070739E-5 0.0 0.0 0.5038831843770716 0.0 70-71 6.143793018070739E-5 0.0 0.0 0.6166217862586696 0.0 72-73 6.143793018070739E-5 0.0 0.0 0.7392826138644519 0.0 74-75 6.143793018070739E-5 0.0 0.0 0.8620355983655053 0.0 76-77 6.143793018070739E-5 0.0 0.0 0.9740369450849349 0.0 78-79 6.143793018070739E-5 0.0 0.0 1.0809389435993657 0.0 80-81 6.143793018070739E-5 0.0 0.0 1.1868886541959955 0.0 82-83 6.143793018070739E-5 0.0 0.0 1.3275507953447252 0.0 84-85 6.143793018070739E-5 0.0 0.0 1.5385593665503645 0.0 86-87 6.143793018070739E-5 0.0 0.0 1.8053535783600865 0.0 88-89 6.143793018070739E-5 0.0 0.0 2.1141098964832317 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 13545 0.0 28.725197 1 GTACATG 11435 0.0 27.045992 1 TACATGG 11610 0.0 26.229937 2 GGTATCA 9585 0.0 25.52544 1 ACATGGG 11585 0.0 25.17931 3 GAGTACT 7100 0.0 24.154995 12-13 ATCAACG 16270 0.0 23.768856 3 TCAACGC 16325 0.0 23.719337 4 CAACGCA 16400 0.0 23.523232 5 TATCAAC 16605 0.0 23.232107 2 CATGGGG 4015 0.0 23.193628 4 AACGCAG 16695 0.0 23.080538 6 GTACTTT 7665 0.0 22.283577 14-15 AGTACTT 7350 0.0 21.45897 12-13 CATGGGA 7110 0.0 21.383488 4 ACGCAGA 18240 0.0 21.045439 7 CGCAGAG 18375 0.0 20.864964 8 TAAGGTG 550 0.0 20.731627 5 ATGGGAG 2670 0.0 20.641037 5 GCAGAGT 18780 0.0 19.959648 9 >>END_MODULE