FastQCFastQC Report
Thu 26 May 2016
SRR936519_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936519_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1627659
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102420.629247280910805No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87670.5386263338942616No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55400.3403661332011189No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT30090.1848667319137485No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21410.1315386085168945No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17510.10757781574641863No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA105450.028.929911
GTATCAA148100.028.2028161
TATCGCG350.00839521427.14191490-91
GTACATG111400.025.1239991
TACATGG113900.024.193362
ACATGGG112200.023.710123
GAGTACT97550.023.61491212-13
ATCAACG182850.022.7846933
TCAACGC185350.022.4759924
TATCAAC186100.022.364012
CAACGCA186550.022.305955
CATGGGG38250.022.229694
AACGCAG191000.021.7842486
GTACTTT106850.021.55951714-15
AGTACTT100600.021.48252112-13
CATGGGA67000.020.347874
ATGGGAG24650.019.8487055
ACGCAGA210150.019.7539467
GAGTGCA10100.019.750279
GTAAGGT5550.019.685514
Produced by FastQC (version 0.11.5)