##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936519_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1627659 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.869484947399915 31.0 31.0 34.0 30.0 34.0 2 31.967052681181993 31.0 31.0 34.0 30.0 34.0 3 32.084471010205455 33.0 31.0 34.0 30.0 34.0 4 35.845267344081286 37.0 35.0 37.0 35.0 37.0 5 35.71099536205065 37.0 35.0 37.0 33.0 37.0 6 35.76917769631108 37.0 35.0 37.0 33.0 37.0 7 35.69482551320639 37.0 35.0 37.0 33.0 37.0 8 35.66050014161443 37.0 35.0 37.0 33.0 37.0 9 37.252648128385616 39.0 37.0 39.0 34.0 39.0 10-11 37.153928740602296 39.0 37.0 39.0 33.0 39.0 12-13 37.01642020840974 39.0 37.0 39.0 33.0 39.0 14-15 38.05878350440725 40.0 37.0 41.0 33.0 41.0 16-17 38.046024382256974 40.0 37.0 41.0 33.0 41.0 18-19 38.220176953526504 40.0 37.5 41.0 33.5 41.0 20-21 38.241466732282376 40.0 38.0 41.0 33.5 41.0 22-23 38.140005369675094 40.0 37.0 41.0 33.0 41.0 24-25 38.01075378810918 40.0 37.0 41.0 33.0 41.0 26-27 37.79426372477282 40.0 37.0 41.0 33.0 41.0 28-29 37.77979202031875 40.0 37.0 41.0 33.0 41.0 30-31 37.605240102503046 39.5 36.5 41.0 32.5 41.0 32-33 37.367080574002294 39.0 36.0 41.0 32.0 41.0 34-35 37.159522356955605 39.0 36.0 41.0 31.5 41.0 36-37 36.943858940969825 39.0 35.0 41.0 31.0 41.0 38-39 36.71093115941361 39.0 35.0 41.0 31.0 41.0 40-41 36.435789068840585 39.0 35.0 40.0 30.0 41.0 42-43 36.11011981010765 38.5 35.0 40.0 30.0 41.0 44-45 35.752766703590865 38.0 35.0 40.0 29.0 41.0 46-47 35.26096283066662 38.0 34.5 40.0 27.0 41.0 48-49 34.78012470671068 38.0 34.0 40.0 25.5 41.0 50-51 34.4248801499577 38.0 34.0 40.0 24.0 41.0 52-53 34.12065150009922 38.0 33.0 40.0 23.5 41.0 54-55 33.757502339249186 37.0 33.0 40.0 23.0 41.0 56-57 33.40798011131324 37.0 33.0 40.0 22.0 41.0 58-59 33.031518886941306 36.5 32.0 39.0 20.5 41.0 60-61 32.64567086840671 36.0 31.5 39.0 19.5 40.0 62-63 32.30441634273518 35.5 31.0 39.0 18.0 40.0 64-65 31.972188584955447 35.0 31.0 38.5 17.5 40.0 66-67 31.50567440723149 35.0 30.5 38.0 13.5 40.0 68-69 31.061993636259192 35.0 30.0 37.5 10.0 40.0 70-71 30.55753232095912 34.0 29.5 37.0 7.5 39.0 72-73 30.00167019013196 34.0 29.0 36.0 7.0 39.0 74-75 29.463722130986895 34.0 28.5 36.0 4.0 38.5 76-77 28.122575428882833 31.5 26.5 34.5 2.0 36.5 78-79 28.89059717053756 33.5 28.5 35.0 2.0 37.0 80-81 28.74364071344182 34.0 28.5 35.0 2.0 36.5 82-83 28.339781551295452 33.0 27.5 35.0 2.0 36.0 84-85 27.88789298004066 33.0 26.5 35.0 2.0 36.0 86-87 27.438294814823006 33.0 26.0 35.0 2.0 35.0 88-89 26.916554081659612 32.5 25.5 34.5 2.0 35.0 90-91 26.323242153301152 32.0 24.5 34.0 2.0 35.0 92-93 25.72010015611378 32.0 23.5 34.0 2.0 35.0 94-95 24.94168649575863 31.0 19.5 34.0 2.0 35.0 96-97 24.079881596820954 31.0 12.0 34.0 2.0 35.0 98-99 22.906995875671747 31.0 2.0 34.0 2.0 35.0 100-101 19.118765970021975 24.5 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.30812279170199375 1101 2 0.41644824857178264 1101 3 0.27686444874951377 1101 4 0.1672623729683309 1101 5 0.13510631563686815 1101 6 0.24692981099728684 1101 7 0.21711669364123054 1101 8 0.1549438002579322 1101 9 0.35193225498586145 1101 10-11 0.3301286617529797 1101 12-13 0.3907083011486563 1101 14-15 0.4317513889222582 1101 16-17 0.4594011649075611 1101 18-19 0.49080192778176723 1101 20-21 0.3916244386880052 1101 22-23 0.3802244176064207 1101 24-25 0.4457683881624348 1101 26-27 0.29455297869208863 1101 28-29 0.2963247028607796 1101 30-31 0.48699351352308184 1101 32-33 0.3686168375088883 1101 34-35 0.3966415604529061 1101 36-37 0.4501961917391526 1101 38-39 0.4852657216978855 1101 40-41 0.38901398395872633 1101 42-43 0.3301014667825726 1101 44-45 0.2933999417275359 1101 46-47 0.14097708136635845 1101 48-49 0.20482468694837763 1101 50-51 0.12389075628183832 1101 52-53 0.1538713177773232 1101 54-55 0.29482377497392775 1101 56-57 0.25949483316242805 1101 58-59 0.2313138809523707 1101 60-61 0.24152253824207293 1101 62-63 0.23095448362459337 1101 64-65 0.31110707842200114 1101 66-67 0.2831311026912289 1101 68-69 0.18544540901840278 1101 70-71 0.05743030791858672 1101 72-73 0.20157933782919102 1101 74-75 0.35632045676566193 1101 76-77 0.2786460540913289 1101 78-79 0.43262576666119656 1101 80-81 0.5286506123144115 1101 82-83 0.481982687707486 1101 84-85 0.570101165202594 1101 86-87 0.5067994183402824 1101 88-89 0.26560651771856314 1101 90-91 0.20339338060656686 1101 92-93 0.18170484992026203 1101 94-95 0.1781731824456081 1101 96-97 0.2708487665226649 1101 98-99 -0.04798565588013659 1101 100-101 -0.20201876658991225 1102 1 0.312470038863939 1102 2 0.3954317985002618 1102 3 0.36205558001530136 1102 4 0.2685387372133974 1102 5 0.15141410393160015 1102 6 0.18515948808283866 1102 7 0.3491064522923537 1102 8 0.13000771357929608 1102 9 0.2812216758094337 1102 10-11 0.37025690687578106 1102 12-13 0.37632913536967294 1102 14-15 0.46419481802412577 1102 16-17 0.5314082714602009 1102 18-19 0.4195555038842613 1102 20-21 0.516547019873137 1102 22-23 0.39091772496862376 1102 24-25 0.42282826124883854 1102 26-27 0.3695834136070246 1102 28-29 0.4016839109442927 1102 30-31 0.38913746306199215 1102 32-33 0.3610254186331616 1102 34-35 0.40834777982777126 1102 36-37 0.34284330650921646 1102 38-39 0.3659081647085074 1102 40-41 0.44357731901670405 1102 42-43 0.5087780045066452 1102 44-45 0.5475927316961915 1102 46-47 0.30129470774734557 1102 48-49 0.24151473777468624 1102 50-51 0.44121145658922956 1102 52-53 0.5387708445143957 1102 54-55 0.5166033805374326 1102 56-57 0.5786867100836375 1102 58-59 0.7192861927885019 1102 60-61 0.6149125312420409 1102 62-63 0.5178206765210831 1102 64-65 0.7235433553188564 1102 66-67 0.7280520860433342 1102 68-69 0.7813228095723197 1102 70-71 0.38682016396143837 1102 72-73 0.1461792328287359 1102 74-75 0.3849831085598012 1102 76-77 0.2218982530139293 1102 78-79 0.25923444213369606 1102 80-81 0.47189139814391723 1102 82-83 0.6652684030801623 1102 84-85 0.4771658698317438 1102 86-87 0.6100810630430864 1102 88-89 0.47723607915143873 1102 90-91 0.42347167442523315 1102 92-93 0.3819197965069847 1102 94-95 0.8339232889678101 1102 96-97 0.6411313547078272 1102 98-99 0.21344855144250374 1102 100-101 0.6000764870851008 1103 1 0.3499818787881601 1103 2 0.31186265334278573 1103 3 0.3667145901976383 1103 4 0.20449954706420925 1103 5 0.3196712670857593 1103 6 0.28676083783420125 1103 7 0.2975051025409883 1103 8 0.35284692740832924 1103 9 0.33509037104978034 1103 10-11 0.4389921069705025 1103 12-13 0.3136678164021163 1103 14-15 0.39880735650388033 1103 16-17 0.47386327974814435 1103 18-19 0.5050145969460758 1103 20-21 0.3675075337258491 1103 22-23 0.3768134152362066 1103 24-25 0.38079209028033745 1103 26-27 0.460685710552319 1103 28-29 0.5971580999094854 1103 30-31 0.6120107166731685 1103 32-33 0.5639691197934695 1103 34-35 0.571154137867083 1103 36-37 0.6631472965636789 1103 38-39 0.46809411071844664 1103 40-41 0.4740740038379201 1103 42-43 0.5280208413524932 1103 44-45 0.6088732195010707 1103 46-47 0.47242157253540995 1103 48-49 0.4200045554398528 1103 50-51 0.5281475801980378 1103 52-53 0.681375331845679 1103 54-55 0.8341205484275562 1103 56-57 0.6347280314191792 1103 58-59 0.5586554208254455 1103 60-61 0.5002961447869154 1103 62-63 0.4654171719035105 1103 64-65 0.62595721987012 1103 66-67 0.5638989366234881 1103 68-69 0.5003184879999765 1103 70-71 0.22248497570664227 1103 72-73 0.5020858750262249 1103 74-75 0.484226833399962 1103 76-77 0.379561643968227 1103 78-79 0.40561586529258875 1103 80-81 0.6741261838412882 1103 82-83 0.40709737537473956 1103 84-85 0.5737530116740359 1103 86-87 0.5039331823895203 1103 88-89 0.3898811182231867 1103 90-91 0.6167850772194541 1103 92-93 0.3075325125856061 1103 94-95 0.49551013481199746 1103 96-97 0.7846045964790811 1103 98-99 0.843925996386858 1103 100-101 0.6755397241203873 1104 1 0.22780601229265685 1104 2 0.38705815892760853 1104 3 0.24755135410066487 1104 4 0.17379594828593525 1104 5 0.19053171298604354 1104 6 0.2197835755025963 1104 7 0.18986046529607137 1104 8 0.224965248863775 1104 9 0.3455196718037712 1104 10-11 0.36079466846254604 1104 12-13 0.29525100587284925 1104 14-15 0.34642993481677564 1104 16-17 0.33365183861377545 1104 18-19 0.3562036766680805 1104 20-21 0.325502236631138 1104 22-23 0.25163727577064066 1104 24-25 0.2351540780925916 1104 26-27 0.22476801471474772 1104 28-29 0.29370687301972254 1104 30-31 0.25815888777375307 1104 32-33 0.2139338919003606 1104 34-35 0.36399568243498237 1104 36-37 0.43513817051052683 1104 38-39 0.33785880314759 1104 40-41 0.3362669125183686 1104 42-43 0.3743790887371361 1104 44-45 0.3908701061668509 1104 46-47 0.20909565365916194 1104 48-49 0.32763720413476705 1104 50-51 0.3157292867773549 1104 52-53 0.40788330699802344 1104 54-55 0.2523005389842723 1104 56-57 0.37061511034440997 1104 58-59 0.3101567561654832 1104 60-61 0.10053370251639393 1104 62-63 0.14117281943276794 1104 64-65 0.24023318522505477 1104 66-67 0.3985795985389977 1104 68-69 0.30058592488487434 1104 70-71 0.2929414996911177 1104 72-73 0.30634752789683617 1104 74-75 0.3881743862602427 1104 76-77 0.39848326467263817 1104 78-79 0.5313663485233704 1104 80-81 0.3889871904368327 1104 82-83 0.39874559811831034 1104 84-85 0.33632236082487665 1104 86-87 0.5618288414277792 1104 88-89 0.5476996816629054 1104 90-91 0.8204642709986913 1104 92-93 0.7463311492108069 1104 94-95 0.802729086311853 1104 96-97 0.6192786185822818 1104 98-99 0.8225747576929798 1104 100-101 0.8510747823294089 1105 1 0.16137200613029634 1105 2 0.2242391722692716 1105 3 0.1745809538075065 1105 4 0.150156947186602 1105 5 0.10806841718628846 1105 6 0.03236068630580746 1105 7 0.12137956709208453 1105 8 0.10970722988231785 1105 9 0.1993099998052088 1105 10-11 0.23750086356344724 1105 12-13 0.1573833946869314 1105 14-15 0.1712677988502378 1105 16-17 0.3064768531957114 1105 18-19 0.32024429359787376 1105 20-21 0.11556461913541938 1105 22-23 0.19993861557423287 1105 24-25 0.17995358458344413 1105 26-27 0.20078099860197796 1105 28-29 0.02586107511128688 1105 30-31 0.22603146440752653 1105 32-33 0.2547291658347959 1105 34-35 -0.005710098517219819 1105 36-37 0.119742788593868 1105 38-39 0.25157059467683496 1105 40-41 0.24626708598379565 1105 42-43 0.23402333282901822 1105 44-45 0.036765372078512826 1105 46-47 0.06885373443768117 1105 48-49 0.07321979861379191 1105 50-51 0.14163287902952248 1105 52-53 -0.13175300105054788 1105 54-55 -0.18754327708166585 1105 56-57 -0.15231701913885587 1105 58-59 -0.16823649863138002 1105 60-61 -0.19577722992532642 1105 62-63 -0.08302419826287633 1105 64-65 -0.18430664352119308 1105 66-67 -0.21986530558882933 1105 68-69 -0.20949079020033068 1105 70-71 -0.445224905693248 1105 72-73 -0.23485864226841002 1105 74-75 -0.16995658881060294 1105 76-77 -0.3078782546243026 1105 78-79 -0.28883185741044315 1105 80-81 -0.09227312262489207 1105 82-83 -0.004279638933038399 1105 84-85 0.039032974957539324 1105 86-87 -0.11802932890225648 1105 88-89 -0.13418749208657132 1105 90-91 0.042880015588195874 1105 92-93 -0.14422463401811925 1105 94-95 -0.10044522259104127 1105 96-97 0.1038927772641145 1105 98-99 0.2706798647319921 1105 100-101 0.47897788887837933 1106 1 0.054131194671562355 1106 2 0.24542898290852122 1106 3 0.22046235938264402 1106 4 0.14002899382209222 1106 5 0.18672956571752053 1106 6 0.05282270576995529 1106 7 0.11321004007817237 1106 8 0.08323062758679356 1106 9 0.1164853740242009 1106 10-11 0.14215986425783456 1106 12-13 0.183975668870886 1106 14-15 0.1961452506145278 1106 16-17 0.27317916315088553 1106 18-19 0.17074184263316283 1106 20-21 0.20003281629575298 1106 22-23 0.20232650268415142 1106 24-25 0.22670226279669237 1106 26-27 0.11122110615017533 1106 28-29 0.23689635095053774 1106 30-31 0.24514575534062288 1106 32-33 0.21317346244665458 1106 34-35 0.21853664827755637 1106 36-37 0.3728959580746789 1106 38-39 0.22170059495402228 1106 40-41 0.09412159455772695 1106 42-43 0.3402085272537363 1106 44-45 0.20534853181516866 1106 46-47 0.31041235183344895 1106 48-49 0.3117579760228395 1106 50-51 0.38994115545087027 1106 52-53 0.3200576637909549 1106 54-55 0.2743718869165477 1106 56-57 0.2912866274929442 1106 58-59 0.2171920061913042 1106 60-61 0.17388329529256907 1106 62-63 0.18584400117180166 1106 64-65 0.30830403724073463 1106 66-67 -0.09013883851036297 1106 68-69 -0.1517189896918758 1106 70-71 -0.06731276373416861 1106 72-73 0.2610537512903548 1106 74-75 0.11259536403648696 1106 76-77 0.01422850892955907 1106 78-79 -0.15771697230407966 1106 80-81 -0.049446094564423504 1106 82-83 0.13706465675486967 1106 84-85 0.11102993017492935 1106 86-87 0.2045355024371105 1106 88-89 0.08350842339867981 1106 90-91 0.28165717715996763 1106 92-93 0.2842725482786399 1106 94-95 0.4173126333247872 1106 96-97 0.4103705096260164 1106 98-99 0.5090732301762664 1106 100-101 0.34144543500676505 1107 1 0.09767606931798767 1107 2 0.14907221593575315 1107 3 0.08221012081902046 1107 4 0.08144542353896611 1107 5 0.016081684564710486 1107 6 0.0868265427541246 1107 7 0.006349995608040615 1107 8 -0.024574046179736797 1107 9 0.0791693469194712 1107 10-11 0.10462229657613165 1107 12-13 0.20606174988851933 1107 14-15 0.1324269755597527 1107 16-17 0.14555312364481665 1107 18-19 0.05252845996214006 1107 20-21 0.010546061358020609 1107 22-23 0.042551368130837375 1107 24-25 0.11362252826499741 1107 26-27 0.15187186486502213 1107 28-29 0.16658292914889472 1107 30-31 0.12444699734147946 1107 32-33 0.14292784121905555 1107 34-35 0.14424183588437955 1107 36-37 0.2295928409036634 1107 38-39 0.20545945173420677 1107 40-41 0.3308185055635775 1107 42-43 0.307224692671646 1107 44-45 0.3141335842716799 1107 46-47 0.36504763933781703 1107 48-49 0.27724924489385216 1107 50-51 0.40375697527956333 1107 52-53 0.264923469724927 1107 54-55 0.5071781016052199 1107 56-57 0.6840583466583823 1107 58-59 0.6517397974951464 1107 60-61 0.4645873258870026 1107 62-63 0.0935394191561798 1107 64-65 0.15073338992312202 1107 66-67 0.26051639680341765 1107 68-69 0.2188028704459306 1107 70-71 0.10565512978828906 1107 72-73 0.2817133786724888 1107 74-75 0.4444008070313501 1107 76-77 0.10694218375941844 1107 78-79 0.2456400894691093 1107 80-81 0.3321439891525557 1107 82-83 0.3902476735739917 1107 84-85 0.4698376121736807 1107 86-87 0.7686722038916365 1107 88-89 0.6462153252153477 1107 90-91 0.6251151614083952 1107 92-93 0.7006587671163551 1107 94-95 0.8831684403470952 1107 96-97 0.8455688342298053 1107 98-99 1.050311828673241 1107 100-101 0.84784528087933 1108 1 0.10324249251613082 1108 2 0.2382042638638424 1108 3 0.08066174957272665 1108 4 0.14054135470786377 1108 5 0.20770048242012962 1108 6 0.09798652849882927 1108 7 0.08826976169542178 1108 8 0.196310253778627 1108 9 0.26407401281623777 1108 10-11 0.1864317197864196 1108 12-13 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-2.009486256115096 2316 94-95 -1.9658689566740861 2316 96-97 -1.6465802344212719 2316 98-99 -1.4698365840828131 2316 100-101 -1.068771863512218 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 17.0 4 30.0 5 80.0 6 164.0 7 376.0 8 854.0 9 1495.0 10 2498.0 11 3842.0 12 5635.0 13 7914.0 14 10303.0 15 14019.0 16 18140.0 17 23314.0 18 19689.0 19 13051.0 20 11871.0 21 12436.0 22 13040.0 23 14753.0 24 16845.0 25 20278.0 26 24801.0 27 29989.0 28 36743.0 29 45191.0 30 55270.0 31 67581.0 32 83706.0 33 105725.0 34 138617.0 35 184247.0 36 243345.0 37 274843.0 38 123185.0 39 3771.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.737253887506704 19.43184981231455 15.257423083989316 21.573473216189427 2 16.11108653335693 21.271616134804066 38.79918008612045 23.818117245718557 3 19.417599803388477 25.66622121868424 30.029215243537166 24.886963734390118 4 13.066213008154056 17.347215873624403 36.659711880151576 32.92685923806996 5 13.662163424201015 38.08854683798158 32.90523038054324 15.344059357274167 6 31.288924768640115 36.535601130212164 17.06020732843919 15.115266772708535 7 28.525393894897878 33.960575183315875 19.895555262018057 17.618475659768194 8 27.62225474402596 33.15933758738186 19.30351321495233 19.914894453639842 9 27.582313002907853 16.20849330234404 20.026430597563742 36.18276309718436 10-11 25.59510315121288 26.604681938907348 26.078865413455766 21.721349496424004 12-13 27.06562001008811 24.11091635287244 27.203148816797622 21.62031482024183 14-15 23.605184888916742 25.162826474684714 26.536153590476598 24.69583504592195 16-17 22.13136258126987 28.724712798337677 26.90537981644161 22.238544803950845 18-19 21.931653448584644 26.056727213745862 30.580232687804333 21.431386649865157 20-21 23.121446945871007 24.88438846458911 30.663920783903652 21.33024380563623 22-23 22.400512394383938 25.084992036076496 30.621032437820727 21.89346313171884 24-25 22.40310975278215 25.381907144241694 30.792579624594545 21.422403478381607 26-27 22.138557362787516 25.683313079283238 30.63899172922076 21.539137828708487 28-29 21.62357352426633 25.830142907916997 30.95742252741902 21.588861040397653 30-31 21.90068211734384 25.43899880349153 30.862361591310183 21.79795748785444 32-33 21.624544189432875 25.77035441863609 30.57038145238834 22.0347199395427 34-35 22.537009861821453 25.561971454998815 30.27416066385667 21.626858019323063 36-37 22.632217102662114 25.27912422652369 30.162435016931 21.926223653883195 38-39 22.217293279048057 25.509492806627286 30.32272869844053 21.95048521588413 40-41 22.25400614072678 25.524872400981163 30.36784458646859 21.853276871823464 42-43 22.25858030427537 25.77917380252052 29.70793558147929 22.254310311724815 44-45 22.321909949265244 25.825879794111817 29.48163917958076 22.37057107704218 46-47 22.692468139825355 26.169824269088306 28.830148083843117 22.307559507243223 48-49 22.98976489171699 26.34672558248696 28.261377104052325 22.402132421743723 50-51 22.812563166337664 26.55320840452506 28.091656237501127 22.542572191636147 52-53 23.19869579161173 26.659044244046953 27.78818471247575 22.354075251865567 54-55 22.635314052460654 26.46409749468254 27.939530294447607 22.961058158409198 56-57 22.921447799096892 26.82183803166961 27.506726611698625 22.749987557534883 58-59 23.21862264849957 26.234355329924284 27.58521433912232 22.961807682453824 60-61 22.8568821780554 26.433245659144628 28.29787351692689 22.411998645873076 62-63 22.959973434198186 26.40723027248071 28.14961264861474 22.48318364470636 64-65 22.69966128436692 26.476650862394703 28.067809481211516 22.75587837202687 66-67 22.635716828828407 26.79798936438516 27.752343954714924 22.813949852071506 68-69 22.882046596956265 26.4406992093146 27.99214936240449 22.685104831324647 70-71 22.773703433252653 26.54549207988589 27.913920925733127 22.766883561128324 72-73 22.809265999356118 26.56583691113694 27.75170370625229 22.87319338325465 74-75 22.69167968467991 27.144838645757968 27.287906227295277 22.87557544226684 76-77 22.712868148281036 26.807602914405464 27.637178760155045 22.842350177158448 78-79 22.777924384377723 26.690098463313657 27.718723820097622 22.813253332211 80-81 22.65549496966439 27.34742339298057 27.30730358651409 22.68977805084095 82-83 22.826237884120086 27.092969103277433 27.174192192753083 22.906600819849398 84-85 22.61343766473298 26.873701335803997 27.128519065096945 23.38434193436608 86-87 22.851161411910713 26.731596578508004 27.141880714710297 23.27536129487099 88-89 22.724030390497994 26.875782587271917 26.976697177586335 23.423489844643754 90-91 22.61990268802967 26.828869668440092 27.25020874947544 23.301018894054803 92-93 22.793539169224335 26.5883356931133 27.35758607564956 23.260539062012807 94-95 22.460602050373712 26.430885670146825 27.478497432962584 23.630014846516875 96-97 22.523251198961944 26.45528114628503 27.357559143259824 23.6639085114932 98-99 22.339773895295863 26.543741202823945 27.066149848892856 24.050335052987336 100-101 22.225430693788628 26.56887859720435 27.20400237030957 24.001688338697456 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 949.0 1 700.5 2 693.0 3 878.5 4 675.5 5 610.0 6 959.0 7 1806.5 8 2806.5 9 3368.0 10 2978.0 11 2336.0 12 2255.0 13 2408.0 14 2595.0 15 2708.5 16 2882.5 17 3093.0 18 3277.5 19 3464.0 20 3794.0 21 4232.5 22 4552.5 23 5163.5 24 5987.5 25 6959.0 26 8608.5 27 10463.0 28 12062.0 29 14472.0 30 17659.0 31 20447.0 32 24433.5 33 27597.5 34 29601.5 35 32001.0 36 35074.0 37 38586.0 38 40691.5 39 41376.0 40 41809.5 41 45202.5 42 48829.5 43 51426.0 44 53606.5 45 55326.5 46 56137.5 47 58351.5 48 67794.0 49 68986.0 50 61468.0 51 56809.5 52 52677.0 53 52497.0 54 53424.5 55 50985.0 56 46560.0 57 42079.5 58 39311.0 59 35818.0 60 31091.5 61 26907.5 62 23115.5 63 18305.5 64 13972.0 65 11605.0 66 8759.0 67 6767.0 68 5048.5 69 3306.5 70 2659.0 71 2307.0 72 2027.5 73 1976.5 74 1721.0 75 1379.0 76 1018.0 77 624.5 78 398.5 79 264.5 80 198.5 81 150.0 82 96.5 83 63.0 84 34.5 85 14.5 86 6.5 87 3.5 88 2.5 89 2.5 90 2.5 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01241046189650289 2 0.010505886060900964 3 0.00516078613517942 4 1.8431379054212216E-4 5 0.005590851646444372 6 0.0 7 2.457517207228295E-4 8 0.0020274516959633434 9 0.0 10-11 0.0 12-13 0.0 14-15 3.686275810842443E-4 16-17 0.003501962020300321 18-19 0.001689543079969453 20-21 6.143793018070739E-4 22-23 3.9934654617459803E-4 24-25 3.379086159938906E-4 26-27 6.143793018070738E-5 28-29 1.5359482545176847E-4 30-31 3.9934654617459803E-4 32-33 0.003932027531565272 34-35 0.0011366017083430865 36-37 0.0014437913592466234 38-39 0.005191505100269774 40-41 9.215689527106108E-5 42-43 0.0013209154988852087 44-45 0.004700001658824115 46-47 0.0 48-49 0.0015973861846983921 50-51 7.372551621684886E-4 52-53 3.0718965090353694E-4 54-55 6.143793018070738E-5 56-57 0.01032157227035884 58-59 0.0017202620450598068 60-61 0.00258039306758971 62-63 1.5359482545176847E-4 64-65 0.0024575172072282955 66-67 8.90849987620257E-4 68-69 0.0012594775687045014 70-71 0.0038398706362942118 72-73 0.001904575835601929 74-75 0.006543139564245336 76-77 0.0012287586036141477 78-79 0.0060516361227996775 80-81 0.002088889626144051 82-83 0.0026418309977704175 84-85 0.0015359482545176848 86-87 3.9934654617459803E-4 88-89 0.002764706858131832 90-91 0.006758172319877812 92-93 0.002181046521415112 94-95 2.764706858131832E-4 96-97 6.143793018070738E-5 98-99 3.686275810842443E-4 100-101 0.0014437913592466234 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1627659.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.50871713530321 #Duplication Level Percentage of deduplicated Percentage of total 1 72.59014869475108 33.03484543759026 2 14.374851808824024 13.083621296593485 3 5.300875338971645 7.237081091122953 4 2.522181686454677 4.591250117308316 5 1.3962949845748651 3.1771796745230048 6 0.8235190508207633 2.24863773236013 7 0.5309235565802335 1.6913154969815294 8 0.3865659094458823 1.4073694901699114 9 0.2728519810212936 1.1175429261694654 >10 1.5242366054178094 13.168239945861874 >50 0.15961419408799163 5.08958556841138 >100 0.10801772536155985 9.006587306213735 >500 0.007472815107561868 2.263684356598244 >1k 0.0020380404838805095 1.365135843348157 >5k 2.7173873118406793E-4 0.8846362220664046 >10k+ 1.3586936559203396E-4 0.6332874946812131 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10242 0.629247280910805 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8767 0.5386263338942616 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5540 0.3403661332011189 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3009 0.1848667319137485 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2141 0.1315386085168945 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1751 0.10757781574641863 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 3.0718965090353695E-5 0.0 18-19 0.0 0.0 0.0 6.143793018070739E-5 0.0 20-21 0.0 0.0 0.0 2.1503275563247585E-4 0.0 22-23 0.0 0.0 0.0 3.9934654617459803E-4 0.0 24-25 0.0 0.0 0.0 7.98693092349196E-4 0.0 26-27 0.0 0.0 0.0 0.0011980396385237939 0.0 28-29 0.0 0.0 0.0 0.0022117654865054658 0.0 30-31 0.0 0.0 0.0 0.004208498217378456 0.0 32-33 0.0 0.0 0.0 0.0066967343896971055 0.0 34-35 0.0 0.0 0.0 0.010290853305268487 0.0 36-37 0.0 0.0 0.0 0.01456078945282765 0.0 38-39 0.0 0.0 0.0 0.02098105315671157 0.0 40-41 0.0 0.0 0.0 0.02847648063875787 0.0 42-43 0.0 0.0 0.0 0.03646341156224983 0.0 44-45 0.0 0.0 0.0 0.041317008046525716 0.0 46-47 0.0 0.0 0.0 0.049457533795469445 0.0 48-49 0.0 0.0 0.0 0.05716799403314822 0.0 50-51 0.0 0.0 0.0 0.06395688531811639 0.0 52-53 0.0 0.0 0.0 0.0782104851200405 0.0 54-55 0.0 0.0 0.0 0.10026670205491445 0.0 56-57 0.0 0.0 0.0 0.13473338088629128 0.0 58-59 0.0 0.0 0.0 0.17755561822224436 0.0 60-61 0.0 0.0 0.0 0.23011576749183948 0.0 62-63 0.0 0.0 0.0 0.27035761176020284 0.0 64-65 0.0 0.0 0.0 0.3164667783608237 0.0 66-67 0.0 0.0 0.0 0.3979641927455321 0.0 68-69 0.0 0.0 0.0 0.5055420084919507 0.0 70-71 0.0 0.0 0.0 0.618649237954633 0.0 72-73 0.0 0.0 0.0 0.7415558172811381 0.0 74-75 0.0 0.0 0.0 0.8643088017821914 0.0 76-77 0.0 0.0 0.0 0.9764637433270728 0.0 78-79 0.0 0.0 0.0 1.0835807745971362 0.0 80-81 0.0 0.0 0.0 1.1896840800192177 0.0 82-83 0.0 0.0 0.0 1.3293939332501463 0.0 84-85 0.0 0.0 0.0 1.5382828958645516 0.0 86-87 0.0 0.0 0.0 1.801052923247437 0.0 88-89 0.0 0.0 0.0 2.1075053189888053 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10545 0.0 28.92991 1 GTATCAA 14810 0.0 28.202816 1 TATCGCG 35 0.008395214 27.141914 90-91 GTACATG 11140 0.0 25.123999 1 TACATGG 11390 0.0 24.19336 2 ACATGGG 11220 0.0 23.71012 3 GAGTACT 9755 0.0 23.614912 12-13 ATCAACG 18285 0.0 22.784693 3 TCAACGC 18535 0.0 22.475992 4 TATCAAC 18610 0.0 22.36401 2 CAACGCA 18655 0.0 22.30595 5 CATGGGG 3825 0.0 22.22969 4 AACGCAG 19100 0.0 21.784248 6 GTACTTT 10685 0.0 21.559517 14-15 AGTACTT 10060 0.0 21.482521 12-13 CATGGGA 6700 0.0 20.34787 4 ATGGGAG 2465 0.0 19.848705 5 ACGCAGA 21015 0.0 19.753946 7 GAGTGCA 1010 0.0 19.75027 9 GTAAGGT 555 0.0 19.68551 4 >>END_MODULE