FastQCFastQC Report
Thu 26 May 2016
SRR936518_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936518_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1629618
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87560.5373038343955454No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65660.4029165117223791No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40940.2512245201022571No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT25470.15629429719112087No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16900.10370528553317403No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA129000.030.3899841
GGTATCA88850.027.4898191
GTACATG115450.025.3543781
ATCAACG157800.024.752423
TCAACGC159450.024.466484
TACATGG118600.024.3997552
TATCAAC162350.024.1757872
CAACGCA161150.024.0904315
ACATGGG119050.023.4693363
AACGCAG165950.023.4494636
GAGTACT70950.023.40243112-13
GTGTAAG5500.023.327451
AGTACTT72250.022.65257512-13
GTACTTT78400.021.35908314-15
ACGCAGA182650.021.3034737
CGCAGAG184350.021.081258
CATGGGG43400.021.0215324
TAAGGTG7350.020.6878595
GCAGAGT187250.020.3995449
CATGGGA71300.019.926664
Produced by FastQC (version 0.11.5)