##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936518_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1629618 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.401497774325026 31.0 31.0 34.0 30.0 34.0 2 31.44243068007349 31.0 31.0 34.0 28.0 34.0 3 31.480654975583235 31.0 31.0 34.0 28.0 34.0 4 35.13840605589776 37.0 35.0 37.0 32.0 37.0 5 35.05905187596112 37.0 35.0 37.0 32.0 37.0 6 35.19441120557087 37.0 35.0 37.0 32.0 37.0 7 35.03235604908635 37.0 35.0 37.0 32.0 37.0 8 35.016949984597616 37.0 35.0 37.0 32.0 37.0 9 36.51152908227572 39.0 37.0 39.0 32.0 39.0 10-11 36.39178138680353 39.0 37.0 39.0 32.0 39.0 12-13 36.29390538150659 39.0 35.0 39.0 32.0 39.0 14-15 37.34323718810175 40.0 37.0 41.0 32.0 41.0 16-17 37.29062976722152 39.0 37.0 41.0 32.0 41.0 18-19 37.27277803755236 39.5 37.0 41.0 31.0 41.0 20-21 37.20049207851165 39.0 36.5 41.0 31.0 41.0 22-23 37.09676961103768 39.0 36.0 41.0 31.0 41.0 24-25 36.94135282010876 39.0 36.0 41.0 31.0 41.0 26-27 36.73185495005578 39.0 36.0 40.5 30.0 41.0 28-29 36.824995489740544 39.0 36.0 41.0 30.0 41.0 30-31 36.727790193775476 39.0 36.0 41.0 30.0 41.0 32-33 36.559588811611064 39.0 35.0 41.0 30.0 41.0 34-35 36.378885419773226 39.0 35.0 40.5 30.0 41.0 36-37 36.12514129078103 38.5 35.0 40.0 29.5 41.0 38-39 35.91885951186106 38.0 35.0 40.0 29.0 41.0 40-41 35.743274190638544 38.0 35.0 40.0 28.5 41.0 42-43 35.442813285076625 38.0 35.0 40.0 27.0 41.0 44-45 35.13360891939092 38.0 34.0 40.0 26.5 41.0 46-47 34.71230527645129 38.0 34.0 40.0 24.5 41.0 48-49 34.38485430327844 38.0 33.0 40.0 24.0 41.0 50-51 33.64269233648622 37.5 32.5 39.5 22.5 40.5 52-53 33.80148323103943 37.5 33.0 39.5 23.0 40.5 54-55 34.119667615355254 38.0 33.0 40.0 23.0 41.0 56-57 34.035158546358716 38.0 33.5 40.0 23.0 41.0 58-59 33.79756758945962 37.5 33.0 40.0 22.0 41.0 60-61 33.47552555261417 37.0 33.0 40.0 21.5 41.0 62-63 33.143440364551694 36.5 32.5 40.0 20.0 41.0 64-65 32.829371055057074 36.0 32.0 39.0 19.5 41.0 66-67 32.465780630798136 35.5 32.0 39.0 18.0 41.0 68-69 32.09337126860405 35.0 31.5 39.0 17.0 40.0 70-71 31.70819971306159 35.0 31.0 38.0 14.0 40.0 72-73 31.30590144438758 35.0 31.0 37.0 11.5 39.5 74-75 30.892205105736437 35.0 30.5 37.0 8.0 39.0 76-77 30.48489001716967 35.0 30.0 36.0 7.0 39.0 78-79 30.042790089456545 34.0 30.0 36.0 3.5 37.5 80-81 29.64615357709598 34.0 29.0 35.0 2.0 37.0 82-83 29.26082247496039 34.0 29.0 35.0 2.0 37.0 84-85 28.88119240214578 34.0 29.0 35.0 2.0 36.0 86-87 28.543041988981468 34.0 29.0 35.0 2.0 36.0 88-89 28.184263121786824 34.0 27.5 35.0 2.0 35.5 90-91 27.838731530947744 34.0 27.0 35.0 2.0 35.0 92-93 27.48784531098699 33.5 26.5 35.0 2.0 35.0 94-95 27.067130149519702 33.0 25.5 35.0 2.0 35.0 96-97 26.504356542453507 33.0 25.0 35.0 2.0 35.0 98-99 25.855047317837677 33.0 23.5 35.0 2.0 35.0 100-101 23.74401209363176 31.0 10.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.32262834922844874 1101 2 0.5118891354965882 1101 3 0.5625359771159317 1101 4 0.5393133446269758 1101 5 0.5570983852841636 1101 6 0.41996561562363866 1101 7 0.462421821973372 1101 8 0.5616822058289372 1101 9 0.5840034643837981 1101 10-11 0.5766020221404702 1101 12-13 0.6806192555523083 1101 14-15 0.7948997380138394 1101 16-17 0.8847914027398858 1101 18-19 0.9853409027010684 1101 20-21 0.8611210993489493 1101 22-23 0.9317659010090793 1101 24-25 0.8125668975326406 1101 26-27 0.9723905596028288 1101 28-29 0.9930498979536537 1101 30-31 0.9116028036746044 1101 32-33 0.68772669922852 1101 34-35 0.710616977326346 1101 36-37 0.7608350442520475 1101 38-39 0.6259110532950061 1101 40-41 0.5934607018599038 1101 42-43 0.5867212389779439 1101 44-45 0.6577638443436413 1101 46-47 0.6180421021575739 1101 48-49 0.4820717929464635 1101 50-51 0.6929607911017044 1101 52-53 0.6638611011147262 1101 54-55 0.7036261188459534 1101 56-57 0.6209050490369066 1101 58-59 0.6377622449784184 1101 60-61 0.7181587630947703 1101 62-63 0.8415909660479954 1101 64-65 0.764234521860125 1101 66-67 0.7945421017407384 1101 68-69 0.6831835947334426 1101 70-71 0.8493209308873482 1101 72-73 0.840529890738253 1101 74-75 0.7723294144933561 1101 76-77 0.6796320676338219 1101 78-79 0.7750877762861919 1101 80-81 0.6799679237917111 1101 82-83 0.8572726527309946 1101 84-85 0.7166973697101788 1101 86-87 0.8493732506541569 1101 88-89 0.7560561521206104 1101 90-91 0.7404315681066933 1101 92-93 0.5006432405636367 1101 94-95 0.43774280065705895 1101 96-97 0.3127538671296115 1101 98-99 0.32058550437850286 1101 100-101 0.7353290541218591 1102 1 0.2810652252788408 1102 2 0.24721845236792106 1102 3 0.36253227263661003 1102 4 0.212798647294278 1102 5 0.44806346262840435 1102 6 0.38370707925585634 1102 7 0.2706889345887902 1102 8 0.4259519735732056 1102 9 0.7189818385407492 1102 10-11 0.5860257639216329 1102 12-13 0.5584924005720922 1102 14-15 0.5313131820985006 1102 16-17 0.5373783389313616 1102 18-19 0.6518417498172084 1102 20-21 0.4801877898049227 1102 22-23 0.1698998489331771 1102 24-25 0.3559208348271454 1102 26-27 0.43470353064817147 1102 28-29 0.5271044581298909 1102 30-31 0.4519823842821822 1102 32-33 0.47664924763812166 1102 34-35 0.41387816655493737 1102 36-37 0.277212244920662 1102 38-39 0.26346233954314613 1102 40-41 0.21209797512120332 1102 42-43 0.3492924651530416 1102 44-45 0.5060043678697355 1102 46-47 0.44991147314455304 1102 48-49 0.6113167293390376 1102 50-51 0.5049885172393758 1102 52-53 0.48924706627901315 1102 54-55 0.33051398576895963 1102 56-57 0.4525232336454721 1102 58-59 0.3109683886683712 1102 60-61 0.1913090371884394 1102 62-63 0.4785134358319141 1102 64-65 0.4818717369081682 1102 66-67 0.43301005761660605 1102 68-69 0.5244513809592846 1102 70-71 0.6908194910545156 1102 72-73 0.5058810262367679 1102 74-75 0.3419173025092128 1102 76-77 0.5218524342563136 1102 78-79 0.28692339871054173 1102 80-81 0.4775513936489659 1102 82-83 0.4983821291671049 1102 84-85 0.46283771806731977 1102 86-87 0.2309470949133079 1102 88-89 0.1305803344324019 1102 90-91 0.07071557314781174 1102 92-93 0.06646371091154535 1102 94-95 0.12645804942796346 1102 96-97 0.07880541382532513 1102 98-99 0.0037068003716917985 1102 100-101 0.2921935677048211 1103 1 0.26248570044565156 1103 2 0.4096410713543186 1103 3 0.44337293203378536 1103 4 0.36825789341622794 1103 5 0.37960082665602357 1103 6 0.21229922602888962 1103 7 0.3412652679373025 1103 8 0.30626762492554604 1103 9 0.5740822630434721 1103 10-11 0.5323332955021911 1103 12-13 0.5989226485586769 1103 14-15 0.8279761557334879 1103 16-17 0.8362468459424903 1103 18-19 0.7468427857272815 1103 20-21 0.5262104828478726 1103 22-23 0.5348755821885263 1103 24-25 0.6106059402034418 1103 26-27 0.5551321182398752 1103 28-29 0.7042072453892274 1103 30-31 0.5620658301452224 1103 32-33 0.5645443812117463 1103 34-35 0.3728039645363097 1103 36-37 0.47002817178100287 1103 38-39 0.5161737056073221 1103 40-41 0.4496273810084048 1103 42-43 0.44450215245513647 1103 44-45 0.5274987673431113 1103 46-47 0.8215996833123427 1103 48-49 0.7811615338459106 1103 50-51 0.10284895884564094 1103 52-53 0.43851061502775934 1103 54-55 0.5894698324895344 1103 56-57 0.5166078638255698 1103 58-59 0.5042842707127448 1103 60-61 0.6157776006236091 1103 62-63 0.5574721919169718 1103 64-65 0.8042146275528026 1103 66-67 0.6701148138397812 1103 68-69 0.4171645249684204 1103 70-71 0.5533387949817232 1103 72-73 0.4796309913390857 1103 74-75 0.42763996366669943 1103 76-77 0.2958921603058542 1103 78-79 0.38933155903709604 1103 80-81 0.6580917860605204 1103 82-83 0.6291584298954724 1103 84-85 0.4644999496674238 1103 86-87 0.45842119348786525 1103 88-89 0.3451801278381339 1103 90-91 0.5464400122502546 1103 92-93 0.6366528636840378 1103 94-95 0.34650445232204774 1103 96-97 0.40397942467813763 1103 98-99 0.30541340712700205 1103 100-101 0.46836550526809617 1104 1 0.28918488925494046 1104 2 0.3767955229994442 1104 3 0.4209970989663212 1104 4 0.37977882471155056 1104 5 0.5378597695967784 1104 6 0.38573960984299305 1104 7 0.4548107450556742 1104 8 0.4569841283801992 1104 9 0.5601285377702183 1104 10-11 0.5627962762159839 1104 12-13 0.4577627668098714 1104 14-15 0.5618754314131138 1104 16-17 0.40400217435552577 1104 18-19 0.48388209172664176 1104 20-21 0.6307650919631769 1104 22-23 0.4962777034377339 1104 24-25 0.4604571358378564 1104 26-27 0.46678116206482656 1104 28-29 0.3995052821053875 1104 30-31 0.4637520202370595 1104 32-33 0.342539374141154 1104 34-35 0.40087606550397226 1104 36-37 0.5025285024966735 1104 38-39 0.4306522253593599 1104 40-41 0.2659634616987816 1104 42-43 0.31542789458463005 1104 44-45 0.36856530592083203 1104 46-47 0.6341456209567014 1104 48-49 0.3517054850740209 1104 50-51 0.24760851263769723 1104 52-53 0.44196332197972765 1104 54-55 0.7048876554820183 1104 56-57 0.7551380431335986 1104 58-59 0.6900297533489379 1104 60-61 0.5177835414796235 1104 62-63 0.4297482089826019 1104 64-65 0.449691144618086 1104 66-67 0.4294475392693826 1104 68-69 0.4703280414062263 1104 70-71 0.26849887197657196 1104 72-73 0.18847068514235943 1104 74-75 0.09818349044688901 1104 76-77 0.202541915081067 1104 78-79 0.41690564111542017 1104 80-81 0.4031306973233164 1104 82-83 0.1757635186015527 1104 84-85 0.3726227155681272 1104 86-87 0.4337908345676347 1104 88-89 0.29551229370389365 1104 90-91 0.3730521538658884 1104 92-93 0.2662079856739048 1104 94-95 0.3285358034600243 1104 96-97 0.5302475512569949 1104 98-99 0.7183138648940819 1104 100-101 0.8762049521841639 1105 1 0.23062205893169718 1105 2 0.22571015131605066 1105 3 0.2626820176752105 1105 4 0.3848380021622333 1105 5 0.4354747891442443 1105 6 0.3190673813196767 1105 7 0.4088163026881233 1105 8 0.08963616947598041 1105 9 0.4834660882581474 1105 10-11 0.4582990222947032 1105 12-13 0.3058466367919621 1105 14-15 0.5442615946402327 1105 16-17 0.46376344397955904 1105 18-19 0.366799729078096 1105 20-21 -0.02177322676500637 1105 22-23 -0.13957627740835932 1105 24-25 -0.03266534680598454 1105 26-27 0.2365509223206459 1105 28-29 0.25272665315233667 1105 30-31 0.04308081678504294 1105 32-33 0.09853790693745168 1105 34-35 -0.04865888583682221 1105 36-37 -0.042656830535335644 1105 38-39 -0.10360969162253753 1105 40-41 -0.22486141092414158 1105 42-43 -0.026589151449940118 1105 44-45 0.056564156939806765 1105 46-47 0.07254629851756533 1105 48-49 0.12106989862551387 1105 50-51 0.14494931153151214 1105 52-53 0.2992628012069005 1105 54-55 0.24577715828723257 1105 56-57 0.47176232704455145 1105 58-59 0.24523082854734923 1105 60-61 0.36728322079282094 1105 62-63 0.25259145826150586 1105 64-65 0.2828150282865707 1105 66-67 0.05406480960869686 1105 68-69 0.2049771410542789 1105 70-71 0.32773493336538806 1105 72-73 0.2479415413655488 1105 74-75 0.31459797267675427 1105 76-77 0.40137019381316463 1105 78-79 0.2512394046126012 1105 80-81 0.3058670251884301 1105 82-83 0.3249751078211247 1105 84-85 0.06817618718752527 1105 86-87 0.33442069723201584 1105 88-89 0.38194178590638117 1105 90-91 0.2304802950300342 1105 92-93 0.12689914140659653 1105 94-95 0.07553011276543486 1105 96-97 0.24714464775661682 1105 98-99 -0.11103094839298322 1105 100-101 0.17600662623260988 1106 1 0.011928334642188787 1106 2 0.039224333230496455 1106 3 0.05457169725415767 1106 4 0.1834182432704523 1106 5 0.29608170472153006 1106 6 0.1737981112972804 1106 7 0.42180033910862846 1106 8 0.2758440269149105 1106 9 0.32263311195446676 1106 10-11 0.37109165598923965 1106 12-13 0.28694357121345604 1106 14-15 0.18260657933149815 1106 16-17 0.10951043709323471 1106 18-19 0.13420344427642306 1106 20-21 0.08820670991012491 1106 22-23 0.14314174579683225 1106 24-25 -0.029661595382222572 1106 26-27 0.10171074969245097 1106 28-29 0.13936416530005147 1106 30-31 0.20639784866691713 1106 32-33 -0.0026310603934689425 1106 34-35 0.09733663474725063 1106 36-37 0.12950339043989345 1106 38-39 0.05696430756238868 1106 40-41 0.002534258044931903 1106 42-43 -0.13015056577501127 1106 44-45 -0.23969382397913108 1106 46-47 0.01982468759976541 1106 48-49 -2.653226109572415E-4 1106 50-51 0.29795271463367357 1106 52-53 0.15753822607218382 1106 54-55 0.22283460697075697 1106 56-57 0.2294358521637605 1106 58-59 0.2501940632011639 1106 60-61 0.18050063269357963 1106 62-63 0.3044366920027173 1106 64-65 0.028935944651074408 1106 66-67 0.24984384831071083 1106 68-69 0.11556960128465477 1106 70-71 0.19576719491312033 1106 72-73 0.2467549837932097 1106 74-75 0.0589896463969346 1106 76-77 0.004415569669600217 1106 78-79 0.09373046145520547 1106 80-81 0.13968156482917138 1106 82-83 0.2255912887533036 1106 84-85 0.3061651871910058 1106 86-87 0.4155812003477557 1106 88-89 0.40592442555722386 1106 90-91 0.605083469633211 1106 92-93 0.28730455293116464 1106 94-95 0.2936661741536355 1106 96-97 0.3388533742579689 1106 98-99 0.4016404693804745 1106 100-101 0.6662132157808749 1107 1 0.31884070317972757 1107 2 0.39330024182878986 1107 3 0.5788252805124436 1107 4 0.5365985280994181 1107 5 0.4979290479969123 1107 6 0.41675561591468124 1107 7 0.5875758340485646 1107 8 0.6017900223661954 1107 9 0.7886948096327941 1107 10-11 0.7369967053687603 1107 12-13 0.8035263486774724 1107 14-15 0.9305995349280991 1107 16-17 0.8482834668735819 1107 18-19 0.9869038625140192 1107 20-21 0.9564962097040635 1107 22-23 0.9693056369329369 1107 24-25 0.968458243428401 1107 26-27 1.0068728930838446 1107 28-29 0.6877179271488458 1107 30-31 0.8230905279824796 1107 32-33 0.9303074403621139 1107 34-35 0.7389669218658312 1107 36-37 1.0009008611708907 1107 38-39 0.9079768928508045 1107 40-41 0.7863424335215541 1107 42-43 0.9628876480146147 1107 44-45 0.9307666189863895 1107 46-47 1.1423883390887823 1107 48-49 0.8431779634544085 1107 50-51 0.73760953847016 1107 52-53 0.8421359395031018 1107 54-55 0.9198144048734562 1107 56-57 0.8689358368996238 1107 58-59 0.9426394557186839 1107 60-61 0.9154490398799382 1107 62-63 0.9211917635152389 1107 64-65 0.8184950779528606 1107 66-67 0.8090990724646971 1107 68-69 0.867739781248936 1107 70-71 0.9579689716597208 1107 72-73 0.9537837414443686 1107 74-75 0.7147028254606305 1107 76-77 0.905128786893659 1107 78-79 1.130328350423806 1107 80-81 1.1504139767168873 1107 82-83 1.0792564010210945 1107 84-85 1.0762919177635624 1107 86-87 1.2308854147784771 1107 88-89 1.2777403246917487 1107 90-91 1.1144608069555737 1107 92-93 1.1716027074959072 1107 94-95 1.1794825388446135 1107 96-97 1.293763086834101 1107 98-99 1.3463691873448482 1107 100-101 1.2130815854174308 1108 1 0.191822647271092 1108 2 0.10456378536924049 1108 3 0.14002221252041025 1108 4 0.21871510149921392 1108 5 0.44530553509132886 1108 6 0.34133535184983543 1108 7 0.4569585028219194 1108 8 0.37687312588438004 1108 9 0.6050416658580744 1108 10-11 0.29646218000005575 1108 12-13 0.5117422939337288 1108 14-15 0.4957764657776025 1108 16-17 0.6334542353518486 1108 18-19 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-3.1719155174203095 2316 86-87 -3.2451766420427397 2316 88-89 -3.613043521982153 2316 90-91 -3.7677179031681653 2316 92-93 -4.113397606954667 2316 94-95 -4.00337459839734 2316 96-97 -4.264823772276319 2316 98-99 -4.850620537982575 2316 100-101 -5.482241944919185 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3161.0 3 1320.0 4 1289.0 5 1514.0 6 1756.0 7 2075.0 8 2574.0 9 3253.0 10 3955.0 11 5352.0 12 6882.0 13 8620.0 14 9851.0 15 12244.0 16 15462.0 17 18770.0 18 16353.0 19 10768.0 20 11039.0 21 11771.0 22 12716.0 23 14497.0 24 16549.0 25 18902.0 26 22281.0 27 26325.0 28 31116.0 29 38117.0 30 46596.0 31 56948.0 32 71528.0 33 91237.0 34 120848.0 35 165201.0 36 224483.0 37 281347.0 38 212575.0 39 30335.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.938278120983966 18.650170675694085 14.367751192765954 22.043800010555994 2 15.707217866453268 20.4601132118082 39.7920358978639 24.040633023874634 3 19.286601516568595 25.694766635490847 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22.655058412021447 48-49 23.010783898311583 26.74469373109013 27.489689989387 22.754832381211283 50-51 22.782465047978295 26.987109035334633 27.44105729597181 22.78936862071526 52-53 23.162930523726843 27.008077751619343 27.19524030290878 22.633751421745036 54-55 22.610143353812155 26.826440971066283 27.44678886049352 23.116626814628034 56-57 22.8415821989577 27.26223369084128 26.959914210365632 22.936269899835384 58-59 23.141068695076143 26.62302775767212 27.110452320544088 23.12545122670765 60-61 22.922983365948518 26.730675886056105 27.771017464925634 22.575323283069743 62-63 22.847539273441335 26.88046146293569 27.622545409916544 22.64945385370643 64-65 22.55480790768032 27.032748204264358 27.494745498004058 22.91769839005127 66-67 22.587115326553604 27.269011833840217 27.118787602082524 23.02508523752366 68-69 22.715777238687128 26.931045423331064 27.499964712950593 22.853212625031222 70-71 22.693216098674483 26.96137350381742 27.32959850893421 23.015811888573886 72-73 22.727345251566692 27.01003591179885 27.256423253564794 23.006195583069662 74-75 22.41981810432936 27.608026826865277 26.970738429165408 23.001416639639952 76-77 22.654303070321046 27.193325351672744 27.122723861340027 23.029647716666187 78-79 22.682660248117205 27.276226290700258 27.2287746960842 22.81233876509834 80-81 22.592420049115557 27.843218130895846 26.81975228621812 22.744609533770475 82-83 22.681257273901892 27.616633267701374 26.746511211896106 22.955598246500625 84-85 22.582159610007086 27.444336808603804 26.661994377911043 23.311509203478074 86-87 22.77055615543963 27.29874503024428 26.73252197094597 23.198176843370117 88-89 22.76020630621481 27.308755740572604 26.52392315648877 23.407114796723818 90-91 22.560870641888503 27.396798305653213 26.808858497678774 23.23347255477951 92-93 22.83010790964006 27.15640530326859 26.941199756347917 23.07228703074343 94-95 22.506133006853318 26.90655583529329 27.081455843117762 23.505855314735633 96-97 22.556132129891235 27.027862941134593 26.972111284847088 23.443893644127083 98-99 22.33738956011867 27.198386225094346 26.61295396242443 23.851270252362553 100-101 22.362387397964838 27.093675746042678 26.92586429649493 23.618072559497556 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 692.0 1 517.5 2 495.5 3 632.5 4 565.0 5 581.0 6 923.5 7 1648.0 8 2508.0 9 2991.0 10 2678.5 11 2228.5 12 2227.5 13 2347.5 14 2456.0 15 2506.5 16 2605.5 17 2722.0 18 2796.5 19 2957.5 20 3224.0 21 3570.0 22 4069.0 23 4765.0 24 5632.5 25 6768.0 26 8293.0 27 10088.0 28 11882.0 29 14091.5 30 17043.5 31 20054.0 32 23997.5 33 27258.5 34 29662.0 35 31941.0 36 35055.5 37 38816.5 38 40737.0 39 41303.0 40 41888.5 41 45325.5 42 48903.5 43 51481.0 44 53428.0 45 55622.0 46 56906.0 47 58760.0 48 68115.0 49 69532.0 50 61623.5 51 56633.5 52 53143.0 53 53358.0 54 54265.0 55 51913.0 56 47624.0 57 42651.0 58 39776.0 59 36541.5 60 31909.0 61 27762.5 62 24025.5 63 19006.0 64 14090.5 65 11567.5 66 8782.5 67 6870.0 68 5200.5 69 3436.5 70 2698.5 71 2325.0 72 2006.0 73 1948.5 74 1596.5 75 1229.5 76 950.5 77 601.5 78 361.5 79 240.5 80 175.5 81 126.5 82 80.5 83 45.5 84 34.0 85 25.0 86 13.5 87 8.0 88 4.5 89 4.0 90 4.5 91 4.5 92 3.5 93 2.0 94 0.5 95 0.5 96 0.5 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.013009183747356743 2 0.0065045918736783715 3 0.009204611141997696 4 0.03730935716223066 5 0.04798670608694799 6 0.05921633168018517 7 0.025711547123313562 8 0.0235638045235141 9 0.01442055745579639 10-11 0.045869645524288516 12-13 0.04283212384742927 14-15 0.01816376598687545 16-17 0.02328766618925417 18-19 0.030497944917152364 20-21 0.024944496194813755 22-23 0.014512603567216367 24-25 0.0032216138996991935 26-27 0.002638655194039339 28-29 0.0015954659312796006 30-31 0.007302324839318171 32-33 0.0015341018569996158 34-35 0.0018409222283995391 36-37 0.00675004817079831 38-39 0.006289817613698425 40-41 0.004663669645278833 42-43 0.001073871299899731 44-45 0.0015341018569996158 46-47 0.0018716042655395314 48-49 7.670509284998079E-4 50-51 0.0016568300055595849 52-53 0.0011352353741797157 54-55 0.002976157602579255 56-57 0.0030988857511392237 58-59 0.0019943324140995005 60-61 5.522766685198617E-4 62-63 0.0011045533370397235 64-65 0.003865936679639032 66-67 0.0033136600111191698 68-69 0.007670509284998079 70-71 0.011014851333257242 72-73 0.004295485199598924 74-75 0.0035284342710991167 76-77 0.003006839639719247 78-79 0.03617412178805094 80-81 0.024484265637713866 82-83 0.004694351682418825 84-85 0.009358021327697657 86-87 0.008376196139217904 88-89 0.006044361316578486 90-91 0.012242132818856933 92-93 0.015064880235736227 94-95 0.007087550579338225 96-97 0.004295485199598924 98-99 0.02420812730345394 100-101 0.043353718478809145 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1629618.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.85808027140212 #Duplication Level Percentage of deduplicated Percentage of total 1 73.87045108586196 35.35297977751866 2 13.982320981335597 13.383340798105381 3 5.0785289398910125 7.291459369978284 4 2.336621861173377 4.473049463837942 5 1.2342532469024499 2.9534495482748078 6 0.763981611601921 2.1937615976351936 7 0.5069387253987228 1.6982779948970048 8 0.34015213026175306 1.302322236364433 9 0.2479908440579084 1.0681529149346531 >10 1.3818331510199222 12.480641841886275 >50 0.15050003319097488 5.038999782787296 >100 0.09891805875319387 8.709796048370448 >500 0.0054377910889358855 1.750996554506119 >1k 0.0018125970296452953 1.353383585531075 >5k 2.5894243280647075E-4 0.9493884853725453 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8756 0.5373038343955454 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6566 0.4029165117223791 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4094 0.2512245201022571 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2547 0.15629429719112087 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1690 0.10370528553317403 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 3.0682037139992317E-5 0.0 18-19 0.0 0.0 0.0 6.136407427998463E-5 0.0 20-21 0.0 0.0 0.0 1.2272814855996927E-4 0.0 22-23 0.0 0.0 0.0 1.2272814855996927E-4 0.0 24-25 0.0 0.0 0.0 1.840922228399539E-4 0.0 26-27 0.0 0.0 0.0 6.136407427998463E-4 0.0 28-29 0.0 0.0 0.0 0.0015341018569996158 0.0 30-31 0.0 0.0 0.0 0.0031602498254192086 0.0 32-33 0.0 0.0 0.0 0.005338674462358663 0.0 34-35 0.0 0.0 0.0 0.007578463173578103 0.0 36-37 0.0 0.0 0.0 0.01098416929611725 0.0 38-39 0.0 0.0 0.0 0.016384207832755897 0.0 40-41 0.0 0.0 0.0 0.02221379488935444 0.0 42-43 0.0 0.0 0.0 0.027889971760253016 0.0 44-45 0.0 0.0 0.0 0.03249227733125186 0.0 46-47 0.0 0.0 0.0 0.04025483272766992 0.0 48-49 0.0 0.0 0.0 0.047281019232728164 0.0 50-51 0.0 0.0 0.0 0.05335606258644664 0.0 52-53 0.0 0.0 0.0 0.06661070263092331 0.0 54-55 0.0 0.0 0.0 0.08557220158343856 0.0 56-57 0.0 0.0 0.0 0.11870880169463027 0.0 58-59 0.0 0.0 0.0 0.16399548851325893 0.0 60-61 0.0 0.0 0.0 0.21388448090288645 0.0 62-63 0.0 0.0 0.0 0.2488006391681977 0.0 64-65 0.0 0.0 0.0 0.29012934319576733 0.0 66-67 0.0 0.0 0.0 0.3670492103057281 0.0 68-69 0.0 0.0 0.0 0.46710333341924304 0.0 70-71 0.0 0.0 0.0 0.5716370339551968 0.0 72-73 0.0 0.0 0.0 0.6839946539618487 0.0 74-75 0.0 0.0 0.0 0.7956159050771408 0.0 76-77 0.0 0.0 0.0 0.8995973289445748 0.0 78-79 0.0 0.0 0.0 0.99824007834965 0.0 80-81 0.0 0.0 0.0 1.0959316846033855 0.0 82-83 0.0 0.0 0.0 1.2318837911706915 0.0 84-85 0.0 0.0 0.0 1.4349068309260207 0.0 86-87 0.0 0.0 0.0 1.6993859910727545 0.0 88-89 0.0 0.0 0.0 1.9938415015052606 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 12900 0.0 30.389984 1 GGTATCA 8885 0.0 27.489819 1 GTACATG 11545 0.0 25.354378 1 ATCAACG 15780 0.0 24.75242 3 TCAACGC 15945 0.0 24.46648 4 TACATGG 11860 0.0 24.399755 2 TATCAAC 16235 0.0 24.175787 2 CAACGCA 16115 0.0 24.090431 5 ACATGGG 11905 0.0 23.469336 3 AACGCAG 16595 0.0 23.449463 6 GAGTACT 7095 0.0 23.402431 12-13 GTGTAAG 550 0.0 23.32745 1 AGTACTT 7225 0.0 22.652575 12-13 GTACTTT 7840 0.0 21.359083 14-15 ACGCAGA 18265 0.0 21.303473 7 CGCAGAG 18435 0.0 21.08125 8 CATGGGG 4340 0.0 21.021532 4 TAAGGTG 735 0.0 20.687859 5 GCAGAGT 18725 0.0 20.399544 9 CATGGGA 7130 0.0 19.92666 4 >>END_MODULE