FastQCFastQC Report
Thu 26 May 2016
SRR936518_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936518_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1629618
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104330.6402113869630797No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85690.5258287525051883No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57000.3497752233959124No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT29760.18261948505723427No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20950.1285577356165678No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17460.10714167369285318No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG856.414961E-427.9362415
GGTATCA114700.027.2337951
GTATCAA157150.026.4419881
TAAGGTG6150.025.4833015
GTAAGGT6550.023.9285374
GTACATG110300.023.7959161
GAGTACT97350.023.14819512-13
TACATGG113350.022.837322
ACTTGAC7150.021.9192038
GTACTTT103300.021.7918914-15
ACATGGG111300.021.7629873
ATCAACG190850.021.7501753
TCAACGC191900.021.6311684
AGTACTT99400.021.57189212-13
CAACGCA193650.021.4343765
TATCAAC194500.021.4165822
AACGCAG197750.020.989976
GGTAAGG6850.020.800523
AGGTAAG6400.020.0379812
ACTTTTT115850.019.71813816-17
Produced by FastQC (version 0.11.5)