##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936518_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1629618 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.463789059767382 31.0 31.0 34.0 30.0 34.0 2 31.651699355309034 31.0 31.0 34.0 30.0 34.0 3 31.92055561487416 31.0 31.0 34.0 30.0 34.0 4 35.75517145735994 37.0 35.0 37.0 35.0 37.0 5 35.566729748935025 37.0 35.0 37.0 33.0 37.0 6 35.6800526258301 37.0 35.0 37.0 33.0 37.0 7 35.60942257633384 37.0 35.0 37.0 33.0 37.0 8 35.59224922650584 37.0 35.0 37.0 33.0 37.0 9 37.20276469700261 39.0 37.0 39.0 33.0 39.0 10-11 37.094378253062985 39.0 37.0 39.0 33.0 39.0 12-13 36.955632240193715 39.0 37.0 39.0 33.0 39.0 14-15 38.02939922116717 40.0 37.0 41.0 33.0 41.0 16-17 37.94583515891455 40.0 37.0 41.0 33.0 41.0 18-19 38.0448246153393 40.0 37.0 41.0 33.0 41.0 20-21 38.124954130354475 40.0 37.0 41.0 33.0 41.0 22-23 38.032621755527984 40.0 37.0 41.0 33.0 41.0 24-25 37.9165439385181 40.0 37.0 41.0 33.0 41.0 26-27 37.697638955878006 39.5 37.0 41.0 32.5 41.0 28-29 37.59296473161194 39.0 36.5 41.0 32.0 41.0 30-31 37.531926500566385 39.0 36.5 41.0 32.0 41.0 32-33 37.3103018621542 39.0 36.0 41.0 32.0 41.0 34-35 37.11499136607475 39.0 35.5 41.0 31.0 41.0 36-37 36.84228696541153 39.0 35.0 41.0 31.0 41.0 38-39 36.66076068133759 39.0 35.0 41.0 31.0 41.0 40-41 36.38341470209583 39.0 35.0 40.0 30.0 41.0 42-43 36.084925424240524 38.5 35.0 40.0 30.0 41.0 44-45 35.734941256171695 38.0 35.0 40.0 28.5 41.0 46-47 35.23315402750829 38.0 34.5 40.0 26.5 41.0 48-49 34.71215616175079 38.0 34.0 40.0 24.5 41.0 50-51 34.47421512280792 38.0 34.0 40.0 24.0 41.0 52-53 34.17014785060057 38.0 33.0 40.0 23.5 41.0 54-55 33.87246551032205 37.5 33.0 40.0 23.0 41.0 56-57 33.577916112855895 37.0 33.0 40.0 22.0 41.0 58-59 33.209470256219554 37.0 32.5 40.0 21.0 41.0 60-61 32.82530507149528 36.0 32.0 39.0 20.0 40.5 62-63 32.52121908324527 36.0 31.5 39.0 19.0 40.0 64-65 32.06698594394514 35.0 31.0 39.0 18.0 40.0 66-67 31.659885629638357 35.0 31.0 38.0 16.0 40.0 68-69 31.21651301102467 35.0 30.0 37.5 12.5 40.0 70-71 30.738711464895452 34.0 30.0 37.0 9.0 39.0 72-73 30.331596116390468 34.0 29.0 36.5 7.5 39.0 74-75 29.968606446418733 34.0 29.0 36.0 7.0 39.0 76-77 28.56698011435809 32.5 27.0 34.5 4.0 36.5 78-79 29.36493889979124 34.0 29.0 35.0 2.0 37.0 80-81 29.219663749418576 34.0 29.0 35.0 2.0 37.0 82-83 28.89459922509447 34.0 29.0 35.0 2.0 36.0 84-85 28.438298116491104 33.5 28.5 35.0 2.0 36.0 86-87 28.044006325408777 33.0 27.0 35.0 2.0 35.5 88-89 27.614076734547606 33.0 26.5 35.0 2.0 35.0 90-91 27.14801168126518 33.0 26.0 35.0 2.0 35.0 92-93 26.67503181727251 32.5 25.0 34.5 2.0 35.0 94-95 26.117579395907505 32.0 24.5 34.0 2.0 35.0 96-97 25.40855587014871 32.0 21.5 34.0 2.0 35.0 98-99 24.519414059000333 31.5 10.0 34.0 2.0 35.0 100-101 21.669588210243134 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3889585291757349 1101 2 0.39627251499161176 1101 3 0.25240315424807136 1101 4 0.18639254379063885 1101 5 0.28401737282174366 1101 6 0.21283891404483057 1101 7 0.21859599769533844 1101 8 0.19566834838649072 1101 9 0.3779406713536204 1101 10-11 0.3337777196733853 1101 12-13 0.31746587961449535 1101 14-15 0.44937428515620326 1101 16-17 0.40922112987789205 1101 18-19 0.41575220285695735 1101 20-21 0.30468467788134745 1101 22-23 0.30013978206182657 1101 24-25 0.2785882044279546 1101 26-27 0.3480212831258882 1101 28-29 0.3286217928026929 1101 30-31 0.39408457733470215 1101 32-33 0.3881661843893909 1101 34-35 0.41405407297919083 1101 36-37 0.49588740571275736 1101 38-39 0.5810136824407053 1101 40-41 0.5676523940773919 1101 42-43 0.5984959511818815 1101 44-45 0.5874271629731354 1101 46-47 0.7734681024725276 1101 48-49 0.7806164049744311 1101 50-51 0.7100695373824308 1101 52-53 0.6102966238377547 1101 54-55 0.5812315178455094 1101 56-57 0.6000701484146802 1101 58-59 0.6901539686131031 1101 60-61 0.6572757400154003 1101 62-63 0.2933298509034543 1101 64-65 0.40802242846991277 1101 66-67 0.5081657548305003 1101 68-69 0.28400820062740806 1101 70-71 0.2827107149625476 1101 72-73 0.340056773065875 1101 74-75 0.5019748102063311 1101 76-77 0.8917793817238007 1101 78-79 0.7159262971203724 1101 80-81 0.47233067469713674 1101 82-83 0.5595166075258469 1101 84-85 0.5103363180136675 1101 86-87 0.45937848280588156 1101 88-89 0.24408116435490612 1101 90-91 0.11590545968451593 1101 92-93 0.29130517143186907 1101 94-95 0.21126126814604262 1101 96-97 0.09821315922779661 1101 98-99 0.02159627664205388 1101 100-101 -0.05947144007305383 1102 1 0.4915196852152093 1102 2 0.6192197987388504 1102 3 0.3560559976627573 1102 4 0.26522121767020224 1102 5 0.30376640568803737 1102 6 0.34532508083863434 1102 7 0.2452187297412749 1102 8 0.12420780827340394 1102 9 0.4246019904657885 1102 10-11 0.363731837050949 1102 12-13 0.42107279504576667 1102 14-15 0.5474125743520517 1102 16-17 0.6669228557872415 1102 18-19 0.40866908719328165 1102 20-21 0.47631849690467476 1102 22-23 0.326685967468741 1102 24-25 0.4265396273638231 1102 26-27 0.07821895111835175 1102 28-29 0.27879276586356383 1102 30-31 0.4328871081744907 1102 32-33 0.5500604358691064 1102 34-35 0.6627505120673405 1102 36-37 0.6517543181350831 1102 38-39 0.6123713438709544 1102 40-41 0.8501280144453744 1102 42-43 0.7049649548961909 1102 44-45 0.610989919498877 1102 46-47 0.663065168524902 1102 48-49 0.56597320303419 1102 50-51 0.4137493705750117 1102 52-53 0.23775131389758286 1102 54-55 0.4073060247528488 1102 56-57 0.5278252415067328 1102 58-59 0.4030606013699298 1102 60-61 0.4161553391453765 1102 62-63 0.43261354824598897 1102 64-65 0.41782569037735584 1102 66-67 0.36916253949365796 1102 68-69 0.32581929788662123 1102 70-71 0.2205278165023259 1102 72-73 0.144341948420049 1102 74-75 -0.11732152016669417 1102 76-77 0.1261311696642835 1102 78-79 0.5207219724053722 1102 80-81 0.5867272641569095 1102 82-83 0.5732243150352794 1102 84-85 0.6817064763914971 1102 86-87 0.22574642227620245 1102 88-89 0.2760614051158221 1102 90-91 0.384532942536179 1102 92-93 0.5684342453195974 1102 94-95 0.5126329461164119 1102 96-97 0.34607382242031903 1102 98-99 -0.08311480043152741 1102 100-101 0.05571613132322284 1103 1 0.5240722411212921 1103 2 0.5918269566570977 1103 3 0.36990339708764 1103 4 0.14709220351864616 1103 5 0.14242818618971143 1103 6 0.14779980389798908 1103 7 0.23110390388437452 1103 8 0.2557713271938269 1103 9 0.19220621697891715 1103 10-11 0.15908449463785246 1103 12-13 0.29064730783742476 1103 14-15 0.31108475068892716 1103 16-17 0.23967239623990366 1103 18-19 0.28750213816153547 1103 20-21 0.2353423022652592 1103 22-23 0.25478939978413706 1103 24-25 0.1752418010117438 1103 26-27 0.2020517155582766 1103 28-29 0.2571224086141797 1103 30-31 0.2154113785925702 1103 32-33 0.08727201572749266 1103 34-35 0.09864347390323758 1103 36-37 -0.014105906003536006 1103 38-39 0.03852491423423032 1103 40-41 0.05851124533871399 1103 42-43 0.01086516075576327 1103 44-45 0.048614540950886465 1103 46-47 0.10195051458499194 1103 48-49 0.1652435109125392 1103 50-51 0.0071965413649976995 1103 52-53 0.08487706750379687 1103 54-55 -0.03283931122732042 1103 56-57 -0.01700725697613592 1103 58-59 0.09818932605441688 1103 60-61 0.13118272096713923 1103 62-63 -0.03553331882397259 1103 64-65 -0.11205076448855777 1103 66-67 0.03532986321629039 1103 68-69 -0.018552477751416774 1103 70-71 0.05557897282776736 1103 72-73 0.11060470811388967 1103 74-75 0.015051257585778188 1103 76-77 -0.19474528373225297 1103 78-79 0.13694263447681365 1103 80-81 -0.02715194725837833 1103 82-83 0.09658041390077798 1103 84-85 0.108851140866836 1103 86-87 0.16418140459226294 1103 88-89 0.11953208592180431 1103 90-91 0.07735217549251772 1103 92-93 0.27451680074078766 1103 94-95 0.1981457069122854 1103 96-97 -0.3438955557838419 1103 98-99 -0.5621012542541841 1103 100-101 -0.21828796235831405 1104 1 0.3126129962565791 1104 2 0.34991923304909633 1104 3 0.17539518720380443 1104 4 0.10754250031384771 1104 5 -0.0018968390472551278 1104 6 0.1007048315543102 1104 7 0.01765788906124044 1104 8 -0.03779418386622524 1104 9 -0.02602403328265268 1104 10-11 0.07415140531490749 1104 12-13 0.027617464196957542 1104 14-15 0.1226281069825319 1104 16-17 0.004884079027334565 1104 18-19 0.1613127113006314 1104 20-21 0.022090826070773062 1104 22-23 0.13741593301381982 1104 24-25 0.06356973088728779 1104 26-27 0.17980887617149222 1104 28-29 0.0364077232114397 1104 30-31 -0.05070190231200655 1104 32-33 -0.008661702485781575 1104 34-35 0.1911453538538055 1104 36-37 0.3022338886061178 1104 38-39 0.2777961088641163 1104 40-41 0.3875927895651259 1104 42-43 0.23918294632609616 1104 44-45 0.20027809382047224 1104 46-47 0.2884523987800378 1104 48-49 0.3954187866798904 1104 50-51 0.17850018362331355 1104 52-53 -0.09161438942075506 1104 54-55 0.19295212497002012 1104 56-57 0.18560313308442744 1104 58-59 0.19052231110465812 1104 60-61 -0.013291037894099134 1104 62-63 -0.1380397898861716 1104 64-65 -0.07866209965314397 1104 66-67 0.07965513604787589 1104 68-69 0.11138623904349387 1104 70-71 -0.035589610103343716 1104 72-73 -0.057733281177288376 1104 74-75 -0.009537894515840861 1104 76-77 0.03098991311608046 1104 78-79 0.018453046537924678 1104 80-81 0.051001824562664666 1104 82-83 0.051898251112266536 1104 84-85 0.12447772149318936 1104 86-87 0.24763723831054563 1104 88-89 0.3564145230451494 1104 90-91 0.31441694836690104 1104 92-93 0.4222481973654375 1104 94-95 0.3445522954758218 1104 96-97 0.14648804609533173 1104 98-99 0.3234766068254302 1104 100-101 0.4571612980765636 1105 1 0.4358390984018392 1105 2 0.5339177714776504 1105 3 0.24245815375733315 1105 4 0.1355893945097364 1105 5 0.05362492294892718 1105 6 0.0607692927688106 1105 7 0.20555676746707974 1105 8 0.19438763393157643 1105 9 0.22077033079648345 1105 10-11 0.23250239226284464 1105 12-13 0.10245805364112215 1105 14-15 0.30523653326575584 1105 16-17 0.2115769286621898 1105 18-19 0.22015046630999535 1105 20-21 0.13024148057457552 1105 22-23 0.2778660139423508 1105 24-25 0.1278074150709827 1105 26-27 0.17499716613994565 1105 28-29 0.05918194210499195 1105 30-31 0.03644552701896231 1105 32-33 0.2699637304061895 1105 34-35 0.3161985963106275 1105 36-37 0.4863397919889252 1105 38-39 0.46733837510245024 1105 40-41 0.4667397697233753 1105 42-43 0.38397461959306867 1105 44-45 0.11142496978192185 1105 46-47 0.20419643087265626 1105 48-49 0.24786114578011365 1105 50-51 0.19741348835884764 1105 52-53 0.3965113986556119 1105 54-55 0.3447446972864796 1105 56-57 0.2905261506521484 1105 58-59 0.29455138342216713 1105 60-61 0.36637608146545375 1105 62-63 0.2291094558486222 1105 64-65 0.30621116393873393 1105 66-67 0.48239040786425846 1105 68-69 0.37381558058302744 1105 70-71 0.35784504054517896 1105 72-73 0.23267360735817633 1105 74-75 0.027416418703989365 1105 76-77 0.0414685566426094 1105 78-79 0.27633997047214365 1105 80-81 0.20633392020688746 1105 82-83 0.38774885260242087 1105 84-85 0.5201346506865328 1105 86-87 0.422978113239882 1105 88-89 0.5585600268472781 1105 90-91 0.3653698915395118 1105 92-93 0.5232083920726573 1105 94-95 0.7312442509894126 1105 96-97 0.9355384400367086 1105 98-99 0.8531221827908873 1105 100-101 0.95962083556946 1106 1 0.3781967480669266 1106 2 0.4000588208313438 1106 3 0.1213010990255583 1106 4 0.015056532107223575 1106 5 -0.10984415131672165 1106 6 -0.1594716761225925 1106 7 -0.17731660717719677 1106 8 0.0607961942835189 1106 9 -0.1019628792309959 1106 10-11 -0.06182101042131194 1106 12-13 -0.11339145052306066 1106 14-15 0.09907994876816417 1106 16-17 0.07210251114300803 1106 18-19 -0.030762310475061838 1106 20-21 -0.09031367441112792 1106 22-23 -0.019563198397989368 1106 24-25 -0.04869062910316302 1106 26-27 -0.06771333331841589 1106 28-29 0.04256229885599083 1106 30-31 0.032441047603036566 1106 32-33 -0.024063947229123528 1106 34-35 -0.22648943640373886 1106 36-37 0.016000422242051116 1106 38-39 0.167559385165454 1106 40-41 0.1242804101474917 1106 42-43 -0.05479253089232117 1106 44-45 0.15915304206561842 1106 46-47 0.2396198680406627 1106 48-49 0.018253286251251666 1106 50-51 -0.14001662961768346 1106 52-53 0.08386523419905245 1106 54-55 -0.05028057344649994 1106 56-57 -0.008681069358225102 1106 58-59 -0.05900498023952849 1106 60-61 0.1768438425586325 1106 62-63 -0.1932163920532659 1106 64-65 -0.17208334895091326 1106 66-67 -0.19582259776810318 1106 68-69 -0.2309517231638658 1106 70-71 -0.2568717217357701 1106 72-73 0.03923616069492297 1106 74-75 -0.00989300583387731 1106 76-77 0.08813929867213943 1106 78-79 0.1337870865433537 1106 80-81 0.19029788981930906 1106 82-83 0.36012869035421247 1106 84-85 0.5481325677232896 1106 86-87 0.42635770368332615 1106 88-89 0.18999169833820062 1106 90-91 0.005700657742561788 1106 92-93 0.4458853481597522 1106 94-95 0.6676020608824444 1106 96-97 0.48411850698412096 1106 98-99 0.35773239349063246 1106 100-101 0.39334812064631564 1107 1 0.5540881741960924 1107 2 0.5810053505277359 1107 3 0.41991816739366783 1107 4 0.20806135557737093 1107 5 0.051837499653316854 1107 6 0.3398067113730221 1107 7 0.25382110594844676 1107 8 0.29081466390023536 1107 9 0.36819991288659537 1107 10-11 0.40986857790466047 1107 12-13 0.4644018882036036 1107 14-15 0.6069787627440064 1107 16-17 0.6012062768078366 1107 18-19 0.5966517142223253 1107 20-21 0.3971184001389645 1107 22-23 0.46446096474520715 1107 24-25 0.43546041725404905 1107 26-27 0.4754422235939515 1107 28-29 0.4922028282165769 1107 30-31 0.47852159650172865 1107 32-33 0.5390080766440022 1107 34-35 0.6295790156592034 1107 36-37 0.7676380743111011 1107 38-39 0.8286054752544487 1107 40-41 0.9296100775417528 1107 42-43 0.6668073805756336 1107 44-45 0.5781496534251929 1107 46-47 0.9704032621761769 1107 48-49 1.005666853024259 1107 50-51 0.920040343807436 1107 52-53 0.647109552120682 1107 54-55 0.7933683969868497 1107 56-57 0.6111907499753357 1107 58-59 0.6398294114079306 1107 60-61 0.7812572795863986 1107 62-63 0.6786636164946529 1107 64-65 0.5473467641564085 1107 66-67 0.7839922863007089 1107 68-69 0.9221600638097094 1107 70-71 0.7285101871983315 1107 72-73 0.8259695189596954 1107 74-75 0.9859690749745127 1107 76-77 0.8318228042359195 1107 78-79 0.936398018724752 1107 80-81 0.8084961863406122 1107 82-83 0.828222809078742 1107 84-85 0.8430276648795818 1107 86-87 1.1894476629280568 1107 88-89 1.3830528083468998 1107 90-91 1.1323629232471184 1107 92-93 1.295889354998092 1107 94-95 1.4463328089782408 1107 96-97 1.3161497450590076 1107 98-99 1.4732720573741354 1107 100-101 1.4296122972219578 1108 1 0.35784520943584397 1108 2 0.24054077531302198 1108 3 0.07575697392095648 1108 4 0.12089155731056422 1108 5 -0.013947824571083345 1108 6 -0.003024262667786104 1108 7 -0.024591367463258962 1108 8 0.03876885318639722 1108 9 0.05300548444250808 1108 10-11 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-1.2768084553496912 2316 74-75 -1.405684424074277 2316 76-77 -1.42784753364122 2316 78-79 -1.1864696133051815 2316 80-81 -1.2624639098812658 2316 82-83 -1.1115213853774968 2316 84-85 -1.4037230780099073 2316 86-87 -1.307457160743784 2316 88-89 -1.5664989030503627 2316 90-91 -1.749235035968642 2316 92-93 -1.7681490944461196 2316 94-95 -1.6938518128163267 2316 96-97 -1.9597568552585862 2316 98-99 -2.429074530050986 2316 100-101 -2.9936873112870757 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 8.0 4 35.0 5 76.0 6 183.0 7 387.0 8 883.0 9 1687.0 10 2606.0 11 4116.0 12 6197.0 13 8243.0 14 10380.0 15 14118.0 16 17703.0 17 22381.0 18 18541.0 19 12059.0 20 11005.0 21 11231.0 22 11899.0 23 13379.0 24 15576.0 25 18753.0 26 22769.0 27 28256.0 28 34504.0 29 42648.0 30 52005.0 31 64323.0 32 79926.0 33 100227.0 34 130711.0 35 175499.0 36 236591.0 37 289126.0 38 163289.0 39 8295.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.505308246453374 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21.92860535413821 26.002228743177845 30.653502845452124 21.415663057231818 20-21 23.104279653268435 24.909395944325603 30.61772759014689 21.36859681225907 22-23 22.39884069390226 25.051830346856015 30.68396421075046 21.865364748491267 24-25 22.3567731824268 25.369043542719826 30.803599371140965 21.470583903712402 26-27 22.13872952969931 25.745988167771937 30.61351225471998 21.50177004780878 28-29 21.620895203661224 25.808655770861638 30.938140103999835 21.632308921477303 30-31 21.88058796601412 25.39260734724334 30.839957585151858 21.88684710159068 32-33 21.611536838875463 25.722609596133932 30.581705212288572 22.08414835270203 34-35 22.527838017287614 25.55102276255733 30.314965703366337 21.606173516788722 36-37 22.667870663050994 25.27711247056744 30.14268154909292 21.91233531728864 38-39 22.20125612491294 25.448280728883816 30.404850225359976 21.945612920843267 40-41 22.272422953553978 25.483824250461318 30.383432674529274 21.86032012145543 42-43 22.312622873202372 25.776807185383998 29.6888352656025 22.221734675811128 44-45 22.308345141057558 25.808565749642696 29.548356849574397 22.33473225972534 46-47 22.67629808087639 26.209040511831756 28.816984311468225 22.29767709582363 48-49 23.037217280306354 26.327674081074697 28.21529501652363 22.419813622095326 50-51 22.814878633687037 26.50584924509638 28.11838032611444 22.560891795102147 52-53 23.152742007043486 26.631743793381286 27.876596029310424 22.33891817026481 54-55 22.610048978845384 26.382727239811004 28.06765229867952 22.93957148266409 56-57 22.948001850808623 26.824789298693265 27.49309935577623 22.73410949472188 58-59 23.28444284759184 26.226002849175057 27.61438505100502 22.875169252228083 60-61 22.937143400678412 26.37215660223652 28.28051431613474 22.410185680950327 62-63 22.971161859082535 26.38802806616191 28.175791523940553 22.465018550814996 64-65 22.71964575591404 26.46176318058654 28.03551646786978 22.78307459562964 66-67 22.699183768900006 26.729313318969567 27.729612172097674 22.84189074003276 68-69 22.9045440473535 26.451669120722038 27.969849329489072 22.67393750243539 70-71 22.749619307601606 26.51441629875862 27.912154941422617 22.82380945221716 72-73 22.84418802443272 26.601909159079 27.74797529236913 22.80592752411915 74-75 22.69933812709482 27.11660033210237 27.296765254188404 22.887296286614408 76-77 22.702468853600802 26.818932837915995 27.680063082868365 22.798535225614835 78-79 22.81975820965044 26.631796422087067 27.713068578454518 22.83537678980797 80-81 22.67547031897334 27.310828314357526 27.370758368343672 22.64294299832546 82-83 22.950702967690304 27.074827134816736 27.17826700927737 22.796202888215586 84-85 22.63464138945305 26.88274257604075 27.09446337828463 23.38815265622157 86-87 22.834999145435784 26.73776477299123 27.115675469342932 23.311560612230057 88-89 22.702787970150023 26.86577699430523 27.0104786725378 23.420956363006947 90-91 22.677005000994306 26.758940781058584 27.27987768800375 23.284176529943363 92-93 22.854142964269364 26.55030553847551 27.42295263447927 23.172598862775853 94-95 22.53177032576704 26.379557510610564 27.505756338723096 23.582915824899295 96-97 22.541621317429726 26.429632457155904 27.429949428760708 23.598796796653666 98-99 22.392729714105464 26.47126510142487 27.130400949504196 24.005604234965467 100-101 22.18827077340773 26.573121867426515 27.30944348740772 23.929163871758032 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 949.0 1 734.5 2 746.0 3 866.0 4 634.0 5 558.5 6 984.0 7 1959.5 8 2984.0 9 3484.5 10 3040.5 11 2402.5 12 2338.0 13 2476.0 14 2606.5 15 2760.0 16 2917.5 17 3073.5 18 3267.0 19 3448.5 20 3691.0 21 4038.0 22 4548.0 23 5173.5 24 5887.0 25 6971.5 26 8561.0 27 10491.5 28 12135.0 29 14435.5 30 17707.5 31 20627.5 32 24493.5 33 27815.5 34 29865.0 35 31834.5 36 34954.0 37 38458.5 38 40294.5 39 41048.5 40 41732.5 41 45382.0 42 49157.0 43 51519.5 44 53575.0 45 55149.0 46 55969.5 47 58800.5 48 68793.0 49 69454.5 50 60961.0 51 56429.5 52 52950.0 53 52700.0 54 53406.0 55 50971.0 56 46526.0 57 42224.0 58 39330.0 59 35720.0 60 31250.5 61 26813.5 62 22991.0 63 18497.0 64 14139.5 65 11678.5 66 8908.0 67 6906.5 68 4985.5 69 3214.5 70 2584.5 71 2297.5 72 1982.5 73 1851.5 74 1637.5 75 1356.5 76 1034.0 77 644.0 78 392.0 79 284.5 80 212.5 81 168.5 82 111.0 83 60.0 84 33.5 85 17.5 86 13.0 87 7.5 88 2.0 89 0.0 90 2.5 91 3.5 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.9931775422215513 2 0.010247800404757434 3 0.005400038536638648 4 0.012395543004556895 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0037432085310790627 10-11 3.375024085399155E-4 12-13 6.136407427998463E-5 14-15 9.204611141997696E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0013806916712996544 24-25 0.0 26-27 6.136407427998463E-5 28-29 0.0 30-31 0.0 32-33 0.0034057061225391473 34-35 7.363688913598157E-4 36-37 0.002761383342599309 38-39 1.840922228399539E-4 40-41 0.003006839639719247 42-43 0.008805744659177795 44-45 0.002147742599799462 46-47 0.0012272814855996926 48-49 0.008161421879237957 50-51 3.681844456799078E-4 52-53 0.0016568300055595849 54-55 0.008529606324917864 56-57 0.0036818444567990783 58-59 0.0014420557455796389 60-61 0.00463298760813884 62-63 0.002454562971199385 64-65 0.014297829307236418 66-67 0.003375024085399155 68-69 1.5341018569996158E-4 70-71 0.0013193275970196695 72-73 0.0 74-75 0.0 76-77 9.511431513397618E-4 78-79 0.009112565030577719 80-81 0.013408050230176641 82-83 0.010370528553317403 84-85 0.006964822430778256 86-87 0.008130739842097964 88-89 0.02479108600911379 90-91 0.020986513403754743 92-93 0.002209106674079447 94-95 0.00675004817079831 96-97 0.008253467990657935 98-99 0.00834551410207791 100-101 0.010247800404757434 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1629618.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.955569548796674 #Duplication Level Percentage of deduplicated Percentage of total 1 72.43491945093864 33.287879785890965 2 14.750262586387228 13.557134363034635 3 5.245222930941109 7.231416216054219 4 2.506267071424663 4.607077228348603 5 1.366633838872299 3.1402218215017466 6 0.7921050972118824 2.1840984530926204 7 0.5321931510600877 1.712006755685458 8 0.3521148372905506 1.2945310315415297 9 0.26390949392240404 1.0915299992284895 >10 1.4817913005117187 12.897625478233458 >50 0.16031653689860373 5.142932675865235 >100 0.10507951468284785 8.88100362765604 >500 0.006753080777894158 2.0987166002451096 >1k 0.0020259242333682476 1.3406139605682466 >5k 2.701232311157663E-4 0.8856530674266545 >10k+ 1.3506161555788316E-4 0.6475589356270437 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10433 0.6402113869630797 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8569 0.5258287525051883 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5700 0.3497752233959124 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2976 0.18261948505723427 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2095 0.1285577356165678 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1746 0.10714167369285318 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 3.0682037139992317E-5 0.0 18-19 0.0 0.0 0.0 6.136407427998463E-5 0.0 20-21 0.0 0.0 0.0 1.2272814855996927E-4 0.0 22-23 0.0 0.0 0.0 1.2272814855996927E-4 0.0 24-25 0.0 0.0 0.0 1.2272814855996927E-4 0.0 26-27 0.0 0.0 0.0 6.443227799398386E-4 0.0 28-29 0.0 0.0 0.0 0.0015647838941396082 0.0 30-31 0.0 0.0 0.0 0.0031602498254192086 0.0 32-33 0.0 0.0 0.0 0.005400038536638648 0.0 34-35 0.0 0.0 0.0 0.00767050928499808 0.0 36-37 0.0 0.0 0.0 0.011290989667517173 0.0 38-39 0.0 0.0 0.0 0.016844438389855783 0.0 40-41 0.0 0.0 0.0 0.022858117669294277 0.0 42-43 0.0 0.0 0.0 0.028841114911592778 0.0 44-45 0.0 0.0 0.0 0.03359683066829158 0.0 46-47 0.0 0.0 0.0 0.04160484236182958 0.0 48-49 0.0 0.0 0.0 0.04884580312686777 0.0 50-51 0.0 0.0 0.0 0.05492084648058625 0.0 52-53 0.0 0.0 0.0 0.06835957874790288 0.0 54-55 0.0 0.0 0.0 0.0874744878861181 0.0 56-57 0.0 0.0 0.0 0.12033494966304988 0.0 58-59 0.0 0.0 0.0 0.1657443646302385 0.0 60-61 0.0 0.0 0.0 0.2153572186856061 0.0 62-63 0.0 0.0 0.0 0.25061087935945725 0.0 64-65 0.0 0.0 0.0 0.29160208097848694 0.0 66-67 0.0 0.0 0.0 0.3686446762370077 0.0 68-69 0.0 0.0 0.0 0.46759424601348293 0.0 70-71 0.0 0.0 0.0 0.5713915776580769 0.0 72-73 0.0 0.0 0.0 0.6849151150760485 0.0 74-75 0.0 0.0 0.0 0.7973034171198403 0.0 76-77 0.0 0.0 0.0 0.9013768870986942 0.0 78-79 0.0 0.0 0.0 1.0006639592837094 0.0 80-81 0.0 0.0 0.0 1.098202155351745 0.0 82-83 0.0 0.0 0.0 1.2332951648791313 0.0 84-85 0.0 0.0 0.0 1.4355204716688204 0.0 86-87 0.0 0.0 0.0 1.6996007653327343 0.0 88-89 0.0 0.0 0.0 1.992798312242501 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 85 6.414961E-4 27.936241 5 GGTATCA 11470 0.0 27.233795 1 GTATCAA 15715 0.0 26.441988 1 TAAGGTG 615 0.0 25.483301 5 GTAAGGT 655 0.0 23.928537 4 GTACATG 11030 0.0 23.795916 1 GAGTACT 9735 0.0 23.148195 12-13 TACATGG 11335 0.0 22.83732 2 ACTTGAC 715 0.0 21.919203 8 GTACTTT 10330 0.0 21.79189 14-15 ACATGGG 11130 0.0 21.762987 3 ATCAACG 19085 0.0 21.750175 3 TCAACGC 19190 0.0 21.631168 4 AGTACTT 9940 0.0 21.571892 12-13 CAACGCA 19365 0.0 21.434376 5 TATCAAC 19450 0.0 21.416582 2 AACGCAG 19775 0.0 20.98997 6 GGTAAGG 685 0.0 20.80052 3 AGGTAAG 640 0.0 20.037981 2 ACTTTTT 11585 0.0 19.718138 16-17 >>END_MODULE