FastQCFastQC Report
Thu 26 May 2016
SRR936517_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936517_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1630569
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85170.5223330015473127No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65360.4008416693804433No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39370.24144945721401548No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT25350.1554672019399363No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17240.10572996297611448No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT16780.10290886187582372No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA131250.028.890971
GGTATCA92100.026.261331
GTACATG112900.024.8743691
TACATGG115100.023.9035172
ATCAACG159400.023.6993983
TCAACGC161400.023.4071644
GAGTACT72900.023.40518412-13
CAACGCA162250.023.3416825
TATCAAC162750.023.2991712
ACATGGG115300.022.7905273
AACGCAG166850.022.6689856
AGTACTT74850.021.9699712-13
GTACTTT78250.021.86536214-15
ACTTGAC7050.021.5684748
GTGTAGC9100.020.887051
CATGGGG40350.020.8456254
ACGCAGA182600.020.6889387
CGCAGAG184650.020.4843548
CGCGGAA3450.019.9609482-83
GCAGAGT187250.019.7685229
Produced by FastQC (version 0.11.5)