##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936517_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1630569 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.409214820102676 31.0 31.0 34.0 30.0 34.0 2 31.43421406883119 31.0 31.0 34.0 28.0 34.0 3 31.48511286550891 31.0 31.0 34.0 28.0 34.0 4 35.126104445748695 37.0 35.0 37.0 32.0 37.0 5 35.09957996257748 37.0 35.0 37.0 32.0 37.0 6 35.24635755984567 37.0 35.0 37.0 32.0 37.0 7 35.102469751356736 37.0 35.0 37.0 32.0 37.0 8 35.04449735031145 37.0 35.0 37.0 32.0 37.0 9 36.50809748008211 39.0 37.0 39.0 32.0 39.0 10-11 36.432067272222156 39.0 37.0 39.0 32.0 39.0 12-13 36.31645578935942 39.0 36.0 39.0 32.0 39.0 14-15 37.352073110674866 40.0 37.0 41.0 32.0 41.0 16-17 37.238130063799815 39.0 37.0 41.0 32.0 41.0 18-19 37.20892706779044 39.0 36.5 41.0 31.0 41.0 20-21 37.13460239953047 39.0 36.5 41.0 31.0 41.0 22-23 37.00545852398764 39.0 36.0 41.0 31.0 41.0 24-25 37.12600601385161 39.0 36.0 41.0 31.0 41.0 26-27 37.108116553178675 39.0 36.0 41.0 31.0 41.0 28-29 37.00292903888152 39.0 36.0 41.0 31.0 41.0 30-31 36.7940010511668 39.0 36.0 41.0 30.0 41.0 32-33 36.5859454583032 39.0 35.0 41.0 30.0 41.0 34-35 36.35432017902953 39.0 35.0 40.0 30.0 41.0 36-37 36.145801557615776 38.5 35.0 40.0 30.0 41.0 38-39 35.92878896875875 38.0 35.0 40.0 29.0 41.0 40-41 35.71460514703763 38.0 35.0 40.0 28.0 41.0 42-43 35.43911082573016 38.0 35.0 40.0 27.0 41.0 44-45 35.131509920769986 38.0 34.0 40.0 26.5 41.0 46-47 34.75471292536531 38.0 34.0 40.0 24.5 41.0 48-49 34.57435533240237 38.0 34.0 40.0 24.0 41.0 50-51 33.625007589375244 37.5 32.5 39.5 22.5 40.5 52-53 33.743403069725964 37.5 33.0 39.5 23.0 40.5 54-55 34.13528620990587 38.0 33.5 40.0 23.0 41.0 56-57 34.04374607882279 38.0 33.5 40.0 22.5 41.0 58-59 33.774364654301664 37.5 33.0 40.0 22.0 41.0 60-61 33.452319711708 37.0 33.0 40.0 21.5 41.0 62-63 33.118784914959136 36.0 32.5 39.5 20.0 41.0 64-65 32.771647504644086 36.0 32.0 39.0 19.5 41.0 66-67 32.38209238615477 35.5 32.0 39.0 18.0 41.0 68-69 31.98215101599503 35.0 31.0 38.5 15.5 40.0 70-71 31.550241970747635 35.0 31.0 38.0 12.5 40.0 72-73 31.16576606080454 35.0 31.0 37.0 9.5 39.5 74-75 30.752867250634594 35.0 30.0 36.5 7.5 39.0 76-77 30.340540326720305 34.5 30.0 36.0 6.5 39.0 78-79 29.89916464743289 34.0 29.5 36.0 2.0 37.5 80-81 29.44352492902784 34.0 29.0 35.0 2.0 37.0 82-83 28.976263807296718 34.0 29.0 35.0 2.0 37.0 84-85 28.67402146121998 34.0 29.0 35.0 2.0 36.0 86-87 28.352134132318227 34.0 28.0 35.0 2.0 36.0 88-89 27.957393094067164 34.0 27.0 35.0 2.0 35.5 90-91 27.55905423198896 33.0 26.5 35.0 2.0 35.0 92-93 27.19682699720159 33.0 26.0 35.0 2.0 35.0 94-95 26.73295303663936 33.0 25.0 35.0 2.0 35.0 96-97 26.149420539701172 33.0 24.5 35.0 2.0 35.0 98-99 25.429930594780103 33.0 21.0 35.0 2.0 35.0 100-101 22.93000112230761 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.48388162789284195 1101 2 0.6148725603846614 1101 3 0.7101222724194294 1101 4 0.6323399537019796 1101 5 0.5758159230266315 1101 6 0.3869654643698226 1101 7 0.5687301499098538 1101 8 0.5569958603650278 1101 9 0.8905316389307671 1101 10-11 0.7705987927695261 1101 12-13 0.860181587261927 1101 14-15 0.9834043125819605 1101 16-17 1.0899469020794328 1101 18-19 0.9662021064137107 1101 20-21 0.9921766721161376 1101 22-23 1.0510659918024814 1101 24-25 0.9824106921625955 1101 26-27 1.0237321587457089 1101 28-29 1.0178947390880069 1101 30-31 1.0281082480497687 1101 32-33 0.9662919839059398 1101 34-35 0.9068767169640566 1101 36-37 0.8190946243880575 1101 38-39 0.898598943338726 1101 40-41 0.9087299728376124 1101 42-43 0.703818259244656 1101 44-45 0.6732603696750772 1101 46-47 0.8332931286669449 1101 48-49 1.069020667064649 1101 50-51 0.9891643993560493 1101 52-53 1.0462195031373867 1101 54-55 1.0874394073562215 1101 56-57 1.0156966219873524 1101 58-59 0.9283335589354991 1101 60-61 0.945715259036831 1101 62-63 0.9910364237568174 1101 64-65 0.9762666590385223 1101 66-67 1.0596976348508562 1101 68-69 1.108417022286453 1101 70-71 0.9684289602204004 1101 72-73 1.0726995851136358 1101 74-75 1.008462416819004 1101 76-77 1.1365766397194896 1101 78-79 1.1337739537611746 1101 80-81 1.153643466997174 1101 82-83 1.2603830525413144 1101 84-85 1.2145725407567376 1101 86-87 1.326444357447759 1101 88-89 1.2232957770614874 1101 90-91 1.0641612917464478 1101 92-93 1.1595240025430549 1101 94-95 1.202391479332281 1101 96-97 0.9993566098508282 1101 98-99 1.035189807566745 1101 100-101 1.1873899921510933 1102 1 0.3508249831236121 1102 2 0.4516598880631868 1102 3 0.3782004271598538 1102 4 0.40387704760856025 1102 5 0.4162502406745503 1102 6 0.4049945030154447 1102 7 0.5891094545794644 1102 8 0.4196073310212114 1102 9 0.6414403420370718 1102 10-11 0.42603554961644363 1102 12-13 0.5788912030198148 1102 14-15 0.602365449979338 1102 16-17 0.5194715793191591 1102 18-19 0.6053960489342245 1102 20-21 0.8003272131312187 1102 22-23 0.6991643022235863 1102 24-25 0.5628590545781194 1102 26-27 0.49575462004141 1102 28-29 0.6168328572367514 1102 30-31 0.5373764662093734 1102 32-33 0.5047213611610957 1102 34-35 0.3382104975453686 1102 36-37 0.4015102137110844 1102 38-39 0.33879834508918805 1102 40-41 0.260596605849976 1102 42-43 0.3653629770319142 1102 44-45 0.22339255138174963 1102 46-47 0.3264731504093916 1102 48-49 0.37250425653232355 1102 50-51 0.6430204740685994 1102 52-53 0.5776466079890028 1102 54-55 0.7892342297596642 1102 56-57 0.5730469507441498 1102 58-59 0.6081516042846715 1102 60-61 0.4748795547725493 1102 62-63 0.49600633784239534 1102 64-65 0.6201410222083155 1102 66-67 0.6968498091329138 1102 68-69 0.7437938582357404 1102 70-71 0.5956896474914686 1102 72-73 0.69597212667043 1102 74-75 0.6333477449447749 1102 76-77 0.6325573121118175 1102 78-79 0.4940871897184387 1102 80-81 0.6557075607332692 1102 82-83 0.778439582915432 1102 84-85 0.8193252517363803 1102 86-87 0.8069768925246237 1102 88-89 0.7194076348159619 1102 90-91 0.8284359566241228 1102 92-93 0.726224840136684 1102 94-95 0.8513757983455612 1102 96-97 0.6112774661462126 1102 98-99 0.7167697221468075 1102 100-101 0.9290701463704671 1103 1 0.38770218035964277 1103 2 0.48255796793584693 1103 3 0.5208503047910433 1103 4 0.5885121636788426 1103 5 0.6299681968255797 1103 6 0.4941942479120627 1103 7 0.5098471461046543 1103 8 0.5076225669479157 1103 9 0.7283336150029669 1103 10-11 0.6861036123240822 1103 12-13 0.5891290711144919 1103 14-15 0.6469813103119932 1103 16-17 0.7554692672251164 1103 18-19 0.6590111555523279 1103 20-21 0.8397570254404769 1103 22-23 0.7065796938125501 1103 24-25 0.6821919205732883 1103 26-27 0.5607966716910653 1103 28-29 0.3339917223429296 1103 30-31 0.42002082714200384 1103 32-33 0.5814393120413897 1103 34-35 0.5744053107945817 1103 36-37 0.6920870249538993 1103 38-39 0.6780015159610215 1103 40-41 0.6237879281182899 1103 42-43 0.7643439690186469 1103 44-45 0.6535319973576321 1103 46-47 0.7943413680765374 1103 48-49 0.5989545421181077 1103 50-51 0.6924525527922398 1103 52-53 0.7239009629305713 1103 54-55 0.7852056342865126 1103 56-57 0.9425501491994694 1103 58-59 0.8068456928083592 1103 60-61 0.6984841726357658 1103 62-63 0.6503865789256409 1103 64-65 0.7188377460975559 1103 66-67 0.5170569137270533 1103 68-69 0.5379347378504846 1103 70-71 0.5850100921303465 1103 72-73 0.5033276076158586 1103 74-75 0.4271534060613611 1103 76-77 0.5259242727095845 1103 78-79 0.5846452020203223 1103 80-81 0.4630758671243811 1103 82-83 0.5650090573005571 1103 84-85 0.7749425334095221 1103 86-87 0.7864393130903125 1103 88-89 0.819027263721356 1103 90-91 0.848192062326877 1103 92-93 0.6679809774638237 1103 94-95 0.853153615946642 1103 96-97 0.9232833131439158 1103 98-99 0.9958586235683242 1103 100-101 1.300743831894053 1104 1 0.3340545875636387 1104 2 0.3424603533786339 1104 3 0.4345512298150531 1104 4 0.40247698651498354 1104 5 0.4871777192534452 1104 6 0.3941123843237406 1104 7 0.4804612266418431 1104 8 0.43180686710059035 1104 9 0.5419540581025899 1104 10-11 0.5412523829885956 1104 12-13 0.4266386564080662 1104 14-15 0.6279652968269502 1104 16-17 0.4237260563762888 1104 18-19 0.6295818863834697 1104 20-21 0.7084680366112295 1104 22-23 0.35160881238004293 1104 24-25 0.3367103070661486 1104 26-27 0.1444168729994999 1104 28-29 0.4154988413663716 1104 30-31 0.41078043143345866 1104 32-33 0.21194731532975197 1104 34-35 0.24066058702374704 1104 36-37 0.2421609439745751 1104 38-39 0.32612020780160833 1104 40-41 0.24027566930499944 1104 42-43 0.20413312710172704 1104 44-45 0.1279411633162013 1104 46-47 0.2202290871566177 1104 48-49 0.038310893972642646 1104 50-51 -0.11338929504511697 1104 52-53 0.2029076156424381 1104 54-55 0.272144592373742 1104 56-57 0.18055316122685383 1104 58-59 0.09897815293007994 1104 60-61 0.16833626551120773 1104 62-63 0.3855723781836886 1104 64-65 0.1783574106295518 1104 66-67 0.17631337127888713 1104 68-69 0.07632806994032393 1104 70-71 0.2736041109071614 1104 72-73 0.28213132843684363 1104 74-75 0.05068590625134206 1104 76-77 0.13171396273328284 1104 78-79 0.09935940483477168 1104 80-81 0.21973306939307236 1104 82-83 0.08251681188940552 1104 84-85 -0.028985364457881246 1104 86-87 0.16055107588625006 1104 88-89 0.23714778626665733 1104 90-91 0.34874887289384304 1104 92-93 0.18343074361720468 1104 94-95 0.10536030596342627 1104 96-97 -0.08165112690223353 1104 98-99 0.17992527503668754 1104 100-101 0.6961083493824631 1105 1 0.4097656765913591 1105 2 0.31665371121989594 1105 3 0.26940032536123226 1105 4 0.2908851755920452 1105 5 0.30985230351045345 1105 6 0.2987319438759286 1105 7 0.31774084637513056 1105 8 0.4582243543659672 1105 9 0.5215480789088502 1105 10-11 0.5623446073959926 1105 12-13 0.4949193602350519 1105 14-15 0.34096868809444203 1105 16-17 0.4607956329543086 1105 18-19 0.4881210353699146 1105 20-21 0.5852679101336307 1105 22-23 0.5427998560982061 1105 24-25 0.6460050207194641 1105 26-27 0.5296744147475323 1105 28-29 0.38128039648489676 1105 30-31 0.37285469880153954 1105 32-33 0.39864649322535683 1105 34-35 0.34837745788455976 1105 36-37 0.42640510133495013 1105 38-39 0.24657639582237323 1105 40-41 0.06644437096380074 1105 42-43 0.35993407081920026 1105 44-45 0.274079426959446 1105 46-47 0.3042632013617208 1105 48-49 0.4098558664880869 1105 50-51 0.6828099488690285 1105 52-53 0.6994346936624325 1105 54-55 0.61686884341092 1105 56-57 0.7116169629210987 1105 58-59 0.6612569039985416 1105 60-61 0.4088209109917216 1105 62-63 0.43899769733113914 1105 64-65 0.5155692745062979 1105 66-67 0.7714234832324038 1105 68-69 0.7796029363396784 1105 70-71 0.7484117885143746 1105 72-73 0.6034203922941295 1105 74-75 0.7599799249728072 1105 76-77 0.6440759318922069 1105 78-79 0.6925283983649564 1105 80-81 0.611639892929027 1105 82-83 0.6509696850851938 1105 84-85 0.8845562515366723 1105 86-87 0.889437308591134 1105 88-89 0.8430401135239691 1105 90-91 0.9109626478332125 1105 92-93 0.8186825521773748 1105 94-95 0.9649915403708924 1105 96-97 1.2162885738601084 1105 98-99 1.2503503648566294 1105 100-101 1.5852986842701284 1106 1 0.2511285049402474 1106 2 0.17165734649563902 1106 3 0.3987298691950052 1106 4 0.3111098321541519 1106 5 0.32729223449239697 1106 6 0.31693128222095623 1106 7 0.4202041370476053 1106 8 0.48456253589319687 1106 9 0.6850610424065096 1106 10-11 0.6215592486864665 1106 12-13 0.6447080272194441 1106 14-15 0.7378924447083861 1106 16-17 0.7566284589724503 1106 18-19 0.6971382585758406 1106 20-21 0.677269085945305 1106 22-23 0.5727672255848475 1106 24-25 0.5418768085446075 1106 26-27 0.28309157394478035 1106 28-29 0.5353108562689997 1106 30-31 0.6129999706819049 1106 32-33 0.3181371371735793 1106 34-35 0.4081641767785982 1106 36-37 0.4862203458376797 1106 38-39 0.5627004961351076 1106 40-41 0.44016324065394485 1106 42-43 0.2884786386040403 1106 44-45 0.5788047636138103 1106 46-47 0.6015863164973396 1106 48-49 0.7260907644394621 1106 50-51 0.5575595670781013 1106 52-53 0.6645212619273408 1106 54-55 0.7914554829898535 1106 56-57 0.6363277932598166 1106 58-59 0.7462940046834987 1106 60-61 0.834410817100725 1106 62-63 0.9636100795325504 1106 64-65 0.7531039313814958 1106 66-67 0.8323743600470053 1106 68-69 0.715084793008927 1106 70-71 0.9056343334856933 1106 72-73 0.8597918652939462 1106 74-75 0.5584289319685389 1106 76-77 0.5159629009071338 1106 78-79 0.6882140739870408 1106 80-81 0.8451019973536233 1106 82-83 1.0773607433504893 1106 84-85 0.9058516620445332 1106 86-87 0.9615818777674718 1106 88-89 1.0926158820630043 1106 90-91 1.3345957667606463 1106 92-93 1.4643904916891692 1106 94-95 1.2068807009874654 1106 96-97 1.312623728889836 1106 98-99 1.6360986924558532 1106 100-101 2.1344352627973002 1107 1 0.09164184200322012 1107 2 0.13580952924698764 1107 3 -0.021657359208326454 1107 4 0.11101972554829587 1107 5 0.12800747258827982 1107 6 0.23103506311307598 1107 7 0.3079179119258555 1107 8 0.3057164822175338 1107 9 0.2985862333587761 1107 10-11 0.341284748247098 1107 12-13 0.3129171719575723 1107 14-15 0.22089869061147027 1107 16-17 0.25385084843457406 1107 18-19 0.45942052631287567 1107 20-21 0.5237119044420595 1107 22-23 0.48175701688523276 1107 24-25 0.4505731783713358 1107 26-27 0.17515086165611393 1107 28-29 0.2780255477722591 1107 30-31 0.29878869775020434 1107 32-33 0.22824970954805934 1107 34-35 0.3325451504846555 1107 36-37 0.3767156209909217 1107 38-39 0.3615647267214257 1107 40-41 0.3665870028212197 1107 42-43 0.3807528427469222 1107 44-45 0.26590061147164334 1107 46-47 0.30436473880644144 1107 48-49 0.471429337305068 1107 50-51 0.46408647830889294 1107 52-53 0.44112976969565665 1107 54-55 0.4822976550461249 1107 56-57 0.5695352569550423 1107 58-59 0.6028670515788477 1107 60-61 0.5869514919126146 1107 62-63 0.48617596660570683 1107 64-65 0.48232766161908813 1107 66-67 0.4928162071241857 1107 68-69 0.617737695192524 1107 70-71 0.6144612970080772 1107 72-73 0.6763258186401053 1107 74-75 0.5986547328992309 1107 76-77 0.43012215283289024 1107 78-79 0.5121835135063613 1107 80-81 0.6024552916903687 1107 82-83 0.7131879957281306 1107 84-85 0.659618449642565 1107 86-87 0.6307000371573999 1107 88-89 0.6665435491880984 1107 90-91 0.45366231103025356 1107 92-93 0.3290068384615985 1107 94-95 0.3187634081633206 1107 96-97 0.7457226181838585 1107 98-99 1.0412073717108399 1107 100-101 1.3939898101029335 1108 1 0.07059130060951091 1108 2 0.30036640104360046 1108 3 0.22252477989081854 1108 4 0.1580714210100922 1108 5 0.2971432792479334 1108 6 0.21547483094173003 1108 7 0.08917041523531566 1108 8 0.23828021053632398 1108 9 0.20640232921431334 1108 10-11 0.10537829564874102 1108 12-13 0.31405358959445806 1108 14-15 0.4168728598521696 1108 16-17 0.40091781832697393 1108 18-19 0.36252581805450035 1108 20-21 0.4014670722910765 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80-81 -3.236733640832824 2316 82-83 -2.951958605288098 2316 84-85 -2.906254237472936 2316 86-87 -3.5010113300515115 2316 88-89 -3.9323103418121583 2316 90-91 -3.558275419941644 2316 92-93 -4.067240631361688 2316 94-95 -4.247492762392749 2316 96-97 -5.750924434148967 2316 98-99 -7.386156523323233 2316 100-101 -7.496592053662766 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3250.0 3 1390.0 4 1361.0 5 1565.0 6 1740.0 7 1966.0 8 2617.0 9 3219.0 10 4228.0 11 5428.0 12 6971.0 13 8757.0 14 9954.0 15 12159.0 16 15425.0 17 19659.0 18 16066.0 19 11221.0 20 11391.0 21 11900.0 22 13226.0 23 14909.0 24 16870.0 25 19783.0 26 22789.0 27 26669.0 28 32118.0 29 38400.0 30 46836.0 31 57200.0 32 72093.0 33 92476.0 34 122161.0 35 166314.0 36 225237.0 37 278836.0 38 206479.0 39 27900.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.89056580622402 18.65697326646114 14.35568030184512 22.096780625469716 2 15.677471199499443 20.494546614484292 39.76720362168595 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22.695728356060584 46-47 22.72328358154034 26.622616387856695 27.971569294249033 22.68253073635393 48-49 22.983188806479774 26.767064252226476 27.50233740077354 22.74740954052021 50-51 22.77487336440846 26.99436299514677 27.409525964780908 22.82123767566386 52-53 23.122109422003994 27.032719150353294 27.186566436452818 22.65860499118989 54-55 22.61685206435734 26.80650050077923 27.37683069614139 23.19981673872204 56-57 22.82867049867417 27.269286752217226 26.96201258293704 22.940030166171567 58-59 23.13728087889792 26.682441883322895 27.061488691003554 23.118788546775633 60-61 22.859204571626243 26.79804306231981 27.714962759241647 22.6277896068123 62-63 22.824347000507828 26.87380863011783 27.594027756311657 22.70781661306269 64-65 22.650222100383363 26.938694406676444 27.444806076897084 22.966277416043113 66-67 22.56109004687015 27.25160107993931 27.059784282001054 23.12752459118949 68-69 22.69361987680659 26.99600437224971 27.440927397836695 22.869448353107007 70-71 22.6507578833484 26.980450939672313 27.295944339702487 23.0728468372768 72-73 22.720016240257685 27.033129389714667 27.192005475567242 23.054848894460402 74-75 22.45388357999697 27.58052319145191 26.954393548454362 23.011199680096755 76-77 22.594431216979892 27.216663416022364 27.13475331289721 23.054152054100534 78-79 22.698782589176055 27.291756939799818 27.151981163696203 22.857479307327925 80-81 22.57458092172227 27.823375154525582 26.83205005417404 22.769993869578112 82-83 22.74411395991826 27.615598711535583 26.696322773584026 22.943964554962136 84-85 22.59329275997394 27.378675555021232 26.649177021624265 23.37885466338056 86-87 22.81033856230997 27.321231709953558 26.64484306170356 23.223586666032915 88-89 22.702444445534788 27.306050027231375 26.511586828844653 23.47991869838918 90-91 22.51779813407846 27.3437480834194 26.836758850615883 23.301694931886257 92-93 22.79310600758516 27.22192200278601 26.883914447070428 23.101057542558408 94-95 22.53355046062881 26.982359940627337 27.001251242041736 23.482838356702118 96-97 22.521326514246145 27.009454905427944 26.971182640583873 23.49803593974204 98-99 22.336432488932537 27.13413268933383 26.647789973065134 23.8816448486685 100-101 22.385314459215795 27.06209427610667 26.8619049459587 23.690686318718836 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 735.0 1 518.0 2 460.5 3 640.5 4 591.0 5 580.0 6 888.5 7 1605.5 8 2499.5 9 2942.0 10 2606.5 11 2180.0 12 2204.0 13 2358.5 14 2425.0 15 2497.5 16 2571.0 17 2612.0 18 2786.0 19 3011.5 20 3238.0 21 3593.5 22 4089.5 23 4764.5 24 5559.5 25 6684.5 26 8312.5 27 9993.5 28 11741.5 29 14085.0 30 17012.5 31 20000.5 32 23929.0 33 27302.5 34 29727.0 35 31950.0 36 34878.0 37 38493.0 38 40668.5 39 41353.5 40 41867.5 41 45271.0 42 49097.5 43 51708.0 44 53695.0 45 55716.5 46 56751.5 47 58816.0 48 68003.0 49 69379.0 50 61911.0 51 57167.0 52 53422.0 53 53077.5 54 54113.5 55 52117.0 56 47736.5 57 42757.5 58 39916.5 59 36861.0 60 32102.0 61 27853.0 62 24111.5 63 18857.0 64 14130.0 65 11766.0 66 8801.0 67 6846.0 68 5109.0 69 3354.0 70 2647.5 71 2231.0 72 2047.5 73 1985.0 74 1604.0 75 1313.5 76 1002.0 77 601.5 78 368.5 79 238.0 80 157.0 81 112.5 82 67.5 83 42.0 84 30.5 85 18.0 86 14.5 87 15.0 88 9.0 89 5.0 90 5.5 91 4.5 92 4.0 93 4.0 94 2.5 95 0.5 96 1.0 97 1.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03642900116462413 2 0.02385670278289358 3 0.02121958653696961 4 0.05194505721622329 5 0.0582005422646941 6 0.05139310265312293 7 0.0544595168925694 8 0.04483097618070747 9 0.04685480957874215 10-11 0.053662249190313316 12-13 0.04792805456254841 14-15 0.04066065281506026 16-17 0.049921223818188615 18-19 0.053662249190313316 20-21 0.012602962524125013 22-23 0.019931692556402092 24-25 0.005764858770159374 26-27 0.010579129126090341 28-29 0.0057035304853704435 30-31 0.009015257863972637 32-33 0.0038023536569136295 34-35 0.007788692168194048 36-37 0.009015257863972637 38-39 0.011131083689190706 40-41 0.001747856116484491 42-43 0.002115825825218068 44-45 0.0022998106795848565 46-47 4.90626278311436E-4 48-49 0.001134573268595196 50-51 2.759772815501828E-4 52-53 0.0034343839481800528 54-55 0.006746111326782246 56-57 0.01631332375385525 58-59 0.010027174562989974 60-61 0.010088502847778904 62-63 0.0057035304853704435 64-65 0.0 66-67 0.006562126472415457 68-69 0.017601217734422768 70-71 0.006562126472415457 72-73 0.003250399093813264 74-75 0.004660949643958643 76-77 0.00754337902903833 78-79 0.006163492621287415 80-81 0.01076311398045713 82-83 0.00619415676368188 84-85 0.016619965177799897 86-87 0.00438497236240846 88-89 0.005948843624526162 90-91 0.0037410253721247 92-93 0.017171919740900262 94-95 0.011591045825107676 96-97 0.008923265436789243 98-99 0.020851616828236032 100-101 0.03753291029082486 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1630569.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.95899465736002 #Duplication Level Percentage of deduplicated Percentage of total 1 74.011660299735 35.49524820897335 2 13.9769654748558 13.406424250694297 3 4.916859532090828 7.07422920091601 4 2.3320716040626555 4.473752383992875 5 1.259172596089079 3.019432590426514 6 0.7619838280897392 2.1926387004210324 7 0.5008577913909259 1.681444529899019 8 0.3460212707548989 1.32758658203737 9 0.23695797418392286 1.022783959611505 >10 1.4050915216943036 12.668261637849316 >50 0.1478152928060309 4.9819166810704605 >100 0.0973153054520416 8.680193869726264 >500 0.005291568939071562 1.7479136979053234 >1k 0.001677814541656837 1.2964358653857078 >5k 2.581253141010518E-4 0.9317378410910029 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8517 0.5223330015473127 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6536 0.4008416693804433 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3937 0.24144945721401548 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2535 0.1554672019399363 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1724 0.10572996297611448 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1678 0.10290886187582372 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.22656569577859E-4 0.0 22-23 0.0 0.0 0.0 2.45313139155718E-4 0.0 24-25 0.0 0.0 0.0 4.599621359169713E-4 0.0 26-27 0.0 0.0 0.0 6.439469902837598E-4 0.0 28-29 0.0 0.0 0.0 0.0016558636893010967 0.0 30-31 0.0 0.0 0.0 0.0030664142394464756 0.0 32-33 0.0 0.0 0.0 0.005396889061425797 0.0 34-35 0.0 0.0 0.0 0.007942012880166372 0.0 36-37 0.0 0.0 0.0 0.011437725113135355 0.0 38-39 0.0 0.0 0.0 0.01830649300949546 0.0 40-41 0.0 0.0 0.0 0.024807291197121988 0.0 42-43 0.0 0.0 0.0 0.031124104530381724 0.0 44-45 0.0 0.0 0.0 0.03664365016138538 0.0 46-47 0.0 0.0 0.0 0.04630285501564178 0.0 48-49 0.0 0.0 0.0 0.053355607766368676 0.0 50-51 0.0 0.0 0.0 0.06007105495075645 0.0 52-53 0.0 0.0 0.0 0.0752498054360165 0.0 54-55 0.0 0.0 0.0 0.09827857637425955 0.0 56-57 0.0 0.0 0.0 0.13378765326704972 0.0 58-59 0.0 0.0 0.0 0.17963054614677454 0.0 60-61 0.0 0.0 0.0 0.2343047120361052 0.0 62-63 0.0 0.0 0.0 0.273217508734681 0.0 64-65 0.0 0.0 0.0 0.32037895973736774 0.0 66-67 0.0 0.0 0.0 0.3978365834257857 0.0 68-69 0.0 0.0 0.0 0.5022173241365437 0.0 70-71 0.0 0.0 0.0 0.6069966986984299 0.0 72-73 0.0 0.0 0.0 0.7192274598621708 0.0 74-75 0.0 0.0 0.0 0.8358125292459258 0.0 76-77 0.0 0.0 0.0 0.9441182801831753 0.0 78-79 0.0 0.0 0.0 1.0449419803761755 0.0 80-81 0.0 0.0 0.0 1.1513772186273625 0.0 82-83 0.0 0.0 0.0 1.2887832406969593 0.0 84-85 0.0 0.0 0.0 1.5016230530569392 0.0 86-87 0.0 0.0 0.0 1.770762230853156 0.0 88-89 0.0 0.0 0.0 2.0667018691021353 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 13125 0.0 28.89097 1 GGTATCA 9210 0.0 26.26133 1 GTACATG 11290 0.0 24.874369 1 TACATGG 11510 0.0 23.903517 2 ATCAACG 15940 0.0 23.699398 3 TCAACGC 16140 0.0 23.407164 4 GAGTACT 7290 0.0 23.405184 12-13 CAACGCA 16225 0.0 23.341682 5 TATCAAC 16275 0.0 23.299171 2 ACATGGG 11530 0.0 22.790527 3 AACGCAG 16685 0.0 22.668985 6 AGTACTT 7485 0.0 21.96997 12-13 GTACTTT 7825 0.0 21.865362 14-15 ACTTGAC 705 0.0 21.568474 8 GTGTAGC 910 0.0 20.88705 1 CATGGGG 4035 0.0 20.845625 4 ACGCAGA 18260 0.0 20.688938 7 CGCAGAG 18465 0.0 20.484354 8 CGCGGAA 345 0.0 19.96094 82-83 GCAGAGT 18725 0.0 19.768522 9 >>END_MODULE