FastQCFastQC Report
Thu 26 May 2016
SRR936517_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936517_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1630569
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106150.6509997430344867No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91300.5599272401229264No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56680.34760871818365247No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT30080.18447548064509997No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22500.13798864077509138No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17650.10824442265246058No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA102050.029.0551091
GTATCAA145050.028.8936081
GTACATG108750.024.2938581
GAGTACT96850.023.37048112-13
TACATGG111650.023.3145072
ATCAACG181950.022.9456983
TCAACGC182400.022.9151294
CAACGCA183700.022.7788185
TATCAAC185000.022.6721862
AGTACTT98300.022.6633312-13
ACATGGG111350.022.436753
AACGCAG187900.022.3707716
AGGTAAG5750.022.3049012
GGTAAGG6250.022.0385193
GTAAGGT6250.022.0385194
GTACTTT103250.021.9678614-15
TACCGAC1100.002878998621.5894587
TATACCG1150.003725386520.6507875
CATGGGA67500.020.5467684
ACGCAGA208500.020.1377377
Produced by FastQC (version 0.11.5)