##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936517_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1630569 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.805106070334958 31.0 31.0 34.0 30.0 34.0 2 31.935182749089428 31.0 31.0 34.0 30.0 34.0 3 32.03028574687732 33.0 31.0 34.0 30.0 34.0 4 35.81104694128246 37.0 35.0 37.0 35.0 37.0 5 35.58682827896274 37.0 35.0 37.0 33.0 37.0 6 35.69725046900806 37.0 35.0 37.0 33.0 37.0 7 35.63885919577767 37.0 35.0 37.0 33.0 37.0 8 35.61683068916433 37.0 35.0 37.0 33.0 37.0 9 37.22529190730352 39.0 37.0 39.0 33.0 39.0 10-11 37.092590377960086 39.0 37.0 39.0 33.0 39.0 12-13 36.98525300063965 39.0 37.0 39.0 33.0 39.0 14-15 38.025125891636606 40.0 37.0 41.0 33.0 41.0 16-17 37.958569983852264 40.0 37.0 41.0 33.0 41.0 18-19 38.14048500860743 40.0 37.0 41.0 33.0 41.0 20-21 38.18988494200491 40.0 37.5 41.0 33.0 41.0 22-23 38.092175185472065 40.0 37.0 41.0 33.0 41.0 24-25 37.95601750063935 40.0 37.0 41.0 33.0 41.0 26-27 37.72045065250229 40.0 37.0 41.0 32.5 41.0 28-29 37.61210902451843 39.0 37.0 41.0 32.0 41.0 30-31 37.52626169147089 39.0 36.5 41.0 32.0 41.0 32-33 37.324665193561266 39.0 36.0 41.0 32.0 41.0 34-35 37.12357281415261 39.0 35.5 41.0 31.0 41.0 36-37 36.824602025427936 39.0 35.0 41.0 31.0 41.0 38-39 36.63320043494019 39.0 35.0 41.0 30.0 41.0 40-41 36.37264537716588 39.0 35.0 40.0 30.0 41.0 42-43 36.05801717069318 38.5 35.0 40.0 30.0 41.0 44-45 35.73648431927751 38.0 35.0 40.0 29.0 41.0 46-47 35.262508057003416 38.0 34.5 40.0 27.0 41.0 48-49 34.81235200718277 38.0 34.0 40.0 25.5 41.0 50-51 34.44089946515603 38.0 34.0 40.0 24.0 41.0 52-53 34.138083086333666 38.0 33.0 40.0 23.5 41.0 54-55 33.84281253967173 37.5 33.0 40.0 23.0 41.0 56-57 33.50655507371967 37.0 33.0 40.0 22.0 41.0 58-59 33.162240910994875 36.5 32.5 39.5 21.0 41.0 60-61 32.781754405977296 36.0 32.0 39.0 19.5 40.5 62-63 32.36978287947336 35.5 31.0 39.0 18.0 40.0 64-65 31.93663960249459 35.0 31.0 38.5 17.5 40.0 66-67 31.47267824912653 35.0 30.5 38.0 13.5 40.0 68-69 31.19036606239908 35.0 30.0 38.0 12.0 40.0 70-71 30.75965813160927 34.5 30.0 37.0 8.0 39.0 72-73 30.314080544889542 34.0 29.0 36.5 7.0 39.0 74-75 29.794288987463887 34.0 29.0 36.0 6.0 39.0 76-77 28.40650993610206 32.5 27.0 34.5 2.0 36.5 78-79 29.18118736465614 34.0 29.0 35.0 2.0 37.0 80-81 28.986192856604042 34.0 29.0 35.0 2.0 37.0 82-83 28.65295826180922 34.0 28.5 35.0 2.0 36.0 84-85 28.23387878709825 33.0 27.5 35.0 2.0 36.0 86-87 27.833231528380587 33.0 27.0 35.0 2.0 35.5 88-89 27.41792834280549 33.0 26.0 35.0 2.0 35.0 90-91 26.912201200930475 32.5 25.5 35.0 2.0 35.0 92-93 26.40345701408527 32.0 25.0 34.0 2.0 35.0 94-95 25.774560904812983 32.0 23.5 34.0 2.0 35.0 96-97 24.970081302907147 31.5 19.0 34.0 2.0 35.0 98-99 23.969211667828837 31.0 6.5 34.0 2.0 35.0 100-101 20.709371084572318 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.41676299678891127 1101 2 0.5021601602729646 1101 3 0.4638022554264012 1101 4 0.29716793944884046 1101 5 0.2600142750101284 1101 6 0.29510050725707515 1101 7 0.29649396734567546 1101 8 0.3873230352176691 1101 9 0.4284970701794748 1101 10-11 0.4066374819493248 1101 12-13 0.45339742567340835 1101 14-15 0.6146490015725661 1101 16-17 0.6333112852452771 1101 18-19 0.517392581077921 1101 20-21 0.42359719224244685 1101 22-23 0.4098179851709389 1101 24-25 0.38979033143760944 1101 26-27 0.4527436286288946 1101 28-29 0.4850672897440731 1101 30-31 0.49202753599153226 1101 32-33 0.5313649717087898 1101 34-35 0.5133282097949348 1101 36-37 0.5257786103368858 1101 38-39 0.5232212840446522 1101 40-41 0.45556287730545364 1101 42-43 0.6378512730761372 1101 44-45 0.6773925298277632 1101 46-47 0.46263698010228893 1101 48-49 0.4119445376217783 1101 50-51 0.4416860016887938 1101 52-53 0.5010977370029437 1101 54-55 0.5383034384136565 1101 56-57 0.5313312600347544 1101 58-59 0.5067426276036784 1101 60-61 0.4741877209094838 1101 62-63 0.5473915752851184 1101 64-65 0.4969728709220469 1101 66-67 0.3930035359900046 1101 68-69 0.49893544872810125 1101 70-71 0.4859191546911248 1101 72-73 0.457141884335055 1101 74-75 0.37535186180755886 1101 76-77 0.23647656125011096 1101 78-79 0.3428138163078778 1101 80-81 0.6271414253868066 1101 82-83 0.6051007209269521 1101 84-85 0.5077257435887148 1101 86-87 0.497808394102389 1101 88-89 0.49585799823912424 1101 90-91 0.2834503035703655 1101 92-93 0.3866510682253903 1101 94-95 0.40691898632549695 1101 96-97 0.3864384567160499 1101 98-99 0.6603413665231415 1101 100-101 1.285379726854515 1102 1 0.3058978966710413 1102 2 0.33756293808148996 1102 3 0.2935926614404849 1102 4 0.1406385247754116 1102 5 0.23322515391146226 1102 6 0.2309883203398897 1102 7 0.2954109039479036 1102 8 0.23391992824642927 1102 9 0.41607486808095473 1102 10-11 0.4467899817039225 1102 12-13 0.37924835924822986 1102 14-15 0.40547145076453006 1102 16-17 0.36911356030574183 1102 18-19 0.3462189315122117 1102 20-21 0.2962097478071044 1102 22-23 0.2857987387468981 1102 24-25 0.22428809630472557 1102 26-27 0.19014651271417193 1102 28-29 0.18151248009384346 1102 30-31 0.3344252058738064 1102 32-33 0.3692043653999306 1102 34-35 0.243176403791324 1102 36-37 0.5063264595980783 1102 38-39 0.5921194303272159 1102 40-41 0.5940427729180655 1102 42-43 0.6061001717464194 1102 44-45 0.5891291361897544 1102 46-47 0.4181404182289299 1102 48-49 0.40169252150019474 1102 50-51 0.14969413924043096 1102 52-53 0.10516186321249421 1102 54-55 0.2492515303400964 1102 56-57 0.12558452933634356 1102 58-59 0.11422237044346417 1102 60-61 0.37676921266601937 1102 62-63 0.29462063046267417 1102 64-65 0.3671417992907813 1102 66-67 0.45320876082068295 1102 68-69 0.41470614335736045 1102 70-71 0.34601577796883376 1102 72-73 0.32261470885368837 1102 74-75 0.18866272941698625 1102 76-77 -0.005512644279523471 1102 78-79 3.0986967651713826E-4 1102 80-81 0.4508314352985927 1102 82-83 0.37376778908355845 1102 84-85 0.22210808747813005 1102 86-87 0.33540405240197657 1102 88-89 0.3808888812291613 1102 90-91 0.4969134273835216 1102 92-93 0.654274742256888 1102 94-95 0.773055671509411 1102 96-97 0.976017578648694 1102 98-99 0.8994171868652501 1102 100-101 0.8927321590052948 1103 1 0.24515834282086502 1103 2 0.26287667835040196 1103 3 0.31757729901622866 1103 4 0.20292837633405725 1103 5 0.03596804237752593 1103 6 0.145954209925101 1103 7 0.21918699626300508 1103 8 0.22407666222033384 1103 9 0.2841593614143605 1103 10-11 0.27139990367953004 1103 12-13 0.23915917847795498 1103 14-15 0.26112562755539415 1103 16-17 0.4024365278678985 1103 18-19 0.35495604677080195 1103 20-21 0.2633061547497064 1103 22-23 0.2658470889202178 1103 24-25 0.38558213106695405 1103 26-27 0.45402390848107643 1103 28-29 0.4768471803034515 1103 30-31 0.35153505318073286 1103 32-33 0.4099111001644431 1103 34-35 0.35760889538890694 1103 36-37 0.4721006157979559 1103 38-39 0.364088803865684 1103 40-41 0.4431564994830808 1103 42-43 0.26523390952051784 1103 44-45 0.36308135876921455 1103 46-47 0.4510146708831826 1103 48-49 0.3828429815471708 1103 50-51 0.2817486175171098 1103 52-53 0.044134878826149304 1103 54-55 0.07824652490246109 1103 56-57 0.22525774400795484 1103 58-59 0.2206036445899997 1103 60-61 0.1708628313918723 1103 62-63 0.23130720292380147 1103 64-65 0.1208721592290054 1103 66-67 0.4176084860398994 1103 68-69 0.5446027843204284 1103 70-71 0.47814316802577395 1103 72-73 0.3083870730049867 1103 74-75 0.5223948814037875 1103 76-77 0.3540550503553952 1103 78-79 0.4567126659750578 1103 80-81 0.47028658225535835 1103 82-83 0.7720256367629439 1103 84-85 0.6451143016700982 1103 86-87 0.37132935560078195 1103 88-89 0.5111637849943946 1103 90-91 0.5149753444186977 1103 92-93 0.7080402404024682 1103 94-95 0.8138418186225422 1103 96-97 0.845128459018909 1103 98-99 1.1156812575211568 1103 100-101 1.7046656212958524 1104 1 0.20019485329041942 1104 2 0.2776535741660311 1104 3 0.19131532171696364 1104 4 0.1102249435900049 1104 5 0.02819100148717979 1104 6 0.2061474356268107 1104 7 0.2681573112133009 1104 8 0.1778292614756154 1104 9 0.29593044733312723 1104 10-11 0.2114709212059438 1104 12-13 0.2212937456371904 1104 14-15 0.28457643686504497 1104 16-17 0.2994410804505776 1104 18-19 0.2517543860107523 1104 20-21 0.1914133442380077 1104 22-23 0.24718547223523757 1104 24-25 0.24645979854189193 1104 26-27 0.31093447337438107 1104 28-29 0.3045246723070676 1104 30-31 0.19720457298343064 1104 32-33 0.27708051979650605 1104 34-35 0.19783569552430436 1104 36-37 0.3930407690244806 1104 38-39 0.5067730660226033 1104 40-41 0.3650902417889341 1104 42-43 0.31861915887631653 1104 44-45 0.44495910914399417 1104 46-47 0.4178818515288327 1104 48-49 0.39720814150931005 1104 50-51 0.26209306633682417 1104 52-53 0.30653842141249044 1104 54-55 0.36672258412057346 1104 56-57 0.5745722659867667 1104 58-59 0.30568063781294086 1104 60-61 0.49740269585064567 1104 62-63 0.44303355747258877 1104 64-65 0.48784641882984303 1104 66-67 0.21649434678199242 1104 68-69 0.48835110041750696 1104 70-71 0.22821432371857497 1104 72-73 0.3186678616587635 1104 74-75 0.3215156250153228 1104 76-77 0.318113308215338 1104 78-79 0.38663449833777364 1104 80-81 0.554088817787612 1104 82-83 0.3800750381570417 1104 84-85 0.2870367488732697 1104 86-87 0.2914165040536645 1104 88-89 0.3768513700530818 1104 90-91 0.463846006857473 1104 92-93 0.5064535720778096 1104 94-95 0.6164522999251787 1104 96-97 0.6474221938637825 1104 98-99 0.9110338581581061 1104 100-101 1.2181891752118332 1105 1 0.1737123898959574 1105 2 0.17214901888767997 1105 3 0.1487827120393419 1105 4 0.05115365053028853 1105 5 0.03678209663029719 1105 6 0.08103294560255847 1105 7 0.15552970435015112 1105 8 0.11455397811715073 1105 9 -0.02070483157777403 1105 10-11 0.019908783873511027 1105 12-13 -0.038099822073498046 1105 14-15 0.08369856419046329 1105 16-17 0.15423576283196638 1105 18-19 0.12184417120730728 1105 20-21 0.08427986867528858 1105 22-23 0.05946682929818081 1105 24-25 0.06484300481851335 1105 26-27 0.068767919055027 1105 28-29 0.12047961292513776 1105 30-31 0.16513763714648633 1105 32-33 0.21263861957482533 1105 34-35 0.28485958033240166 1105 36-37 0.23417969285300444 1105 38-39 0.30368947961824944 1105 40-41 0.41427595537972195 1105 42-43 0.13871849938597336 1105 44-45 0.421996128164281 1105 46-47 0.4604375676908603 1105 48-49 0.4227719049823051 1105 50-51 0.2730618708455381 1105 52-53 0.3810281676928682 1105 54-55 0.5414206362265617 1105 56-57 0.30899241484117823 1105 58-59 0.42148860034220803 1105 60-61 0.4452385042996241 1105 62-63 0.5452179020476038 1105 64-65 0.4398212414484881 1105 66-67 0.6183402595402434 1105 68-69 0.6195299133348975 1105 70-71 0.520635049366426 1105 72-73 0.3119435272297544 1105 74-75 0.1764244499550358 1105 76-77 0.1368391158998925 1105 78-79 0.29154193341376455 1105 80-81 0.46518079896885567 1105 82-83 0.4136150558083109 1105 84-85 0.20678810225307132 1105 86-87 0.3136444208564235 1105 88-89 0.42068471844326893 1105 90-91 0.23217639831259262 1105 92-93 0.5447715937457787 1105 94-95 0.24337448145527674 1105 96-97 0.4107674916073414 1105 98-99 0.6870064152988746 1105 100-101 0.8100305014197993 1106 1 0.0947041070332375 1106 2 0.1729960903165022 1106 3 0.21503378609808976 1106 4 0.12011152655520618 1106 5 0.016033195040172643 1106 6 0.09820014393554999 1106 7 0.1607982451261165 1106 8 0.11135702270406256 1106 9 0.11661292630938647 1106 10-11 0.1649455018978685 1106 12-13 0.1435844759704139 1106 14-15 0.3306807635148985 1106 16-17 0.25374960992774476 1106 18-19 0.15259547312304989 1106 20-21 0.16698447903982583 1106 22-23 0.16466057731964412 1106 24-25 0.098413297832181 1106 26-27 0.11110009628949769 1106 28-29 0.14793585916375918 1106 30-31 0.22063681790491785 1106 32-33 0.19076956603364437 1106 34-35 0.17477821277297778 1106 36-37 0.3256096607442913 1106 38-39 0.4328169090782481 1106 40-41 0.4931741363503548 1106 42-43 0.3615682076942335 1106 44-45 0.05628269225471172 1106 46-47 0.18072431963286562 1106 48-49 0.07568690910460418 1106 50-51 0.07272217259082225 1106 52-53 -0.11230223190916888 1106 54-55 -0.12538373027880567 1106 56-57 0.020479190438564387 1106 58-59 0.05214510679004292 1106 60-61 0.06781165335289074 1106 62-63 0.03694408403403315 1106 64-65 0.11470444299582638 1106 66-67 0.26775131008275466 1106 68-69 0.21574731326045438 1106 70-71 0.3293397084332206 1106 72-73 0.1623484517238616 1106 74-75 0.2387525570721749 1106 76-77 -0.04001896223939738 1106 78-79 0.10040783174432022 1106 80-81 0.12422185149424081 1106 82-83 0.29398578435931455 1106 84-85 0.12500152946580556 1106 86-87 0.11105328675475334 1106 88-89 0.088832571543346 1106 90-91 0.41507373047243235 1106 92-93 0.41581775305086666 1106 94-95 0.6298507338860446 1106 96-97 0.5283277867430272 1106 98-99 0.8608153841676973 1106 100-101 1.0431803895785414 1107 1 -0.052782547786076606 1107 2 0.09145671501504182 1107 3 -0.04457403369411139 1107 4 0.012514971441689227 1107 5 -0.054748858592198246 1107 6 -0.03622071479664157 1107 7 -0.005396015786622854 1107 8 -0.04489405542959446 1107 9 -0.05118661219867704 1107 10-11 -0.05791478753916124 1107 12-13 0.022592173591043263 1107 14-15 0.14569890321936896 1107 16-17 0.08254587496835342 1107 18-19 0.08820205902298994 1107 20-21 0.05563466584867882 1107 22-23 -0.15033709898522574 1107 24-25 -0.07143308799321346 1107 26-27 -0.0635790342223359 1107 28-29 -0.21061875511299633 1107 30-31 -0.15533012000607727 1107 32-33 -0.06750627247529195 1107 34-35 -0.04786492786165297 1107 36-37 -0.039897266708379675 1107 38-39 0.08344072144003434 1107 40-41 -0.04713656232084418 1107 42-43 -0.3320666264913399 1107 44-45 -0.1838495520424317 1107 46-47 0.07967317736928692 1107 48-49 -0.11263589713439615 1107 50-51 -0.10456342002584051 1107 52-53 -0.1348770822895844 1107 54-55 -0.005079404011716804 1107 56-57 -0.22676366242316703 1107 58-59 -0.20938272106575084 1107 60-61 -0.15650441247905178 1107 62-63 -0.058635212823261895 1107 64-65 -0.002630999138755641 1107 66-67 -0.006560899835871936 1107 68-69 0.1695546555958778 1107 70-71 -0.009378768629844103 1107 72-73 0.062182816570373944 1107 74-75 -0.035280437369223705 1107 76-77 0.00820024846191103 1107 78-79 0.10938906562305206 1107 80-81 0.40992439551760995 1107 82-83 0.3719119321192679 1107 84-85 0.28596984400550696 1107 86-87 0.3798444376366419 1107 88-89 0.36675378102835055 1107 90-91 0.48090820761396813 1107 92-93 0.3956969885517907 1107 94-95 0.47120036442866464 1107 96-97 0.5417893213018203 1107 98-99 0.5594233318138642 1107 100-101 0.7120220393953183 1108 1 0.11850729302041074 1108 2 0.1893108272311963 1108 3 0.05946520462094895 1108 4 0.03755790956327587 1108 5 0.07585872431401697 1108 6 0.04401077768494588 1108 7 0.05014656593157696 1108 8 0.15100187478323335 1108 9 0.1208347041178115 1108 10-11 0.02971241452458173 1108 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-1.7341171602705359 2316 66-67 -2.4232502077025266 2316 68-69 -1.785790868410725 2316 70-71 -1.4434943237673465 2316 72-73 -1.2145431754669147 2316 74-75 -1.1701454132115714 2316 76-77 -1.2112041945193504 2316 78-79 -1.3706395871019446 2316 80-81 -2.035689874407705 2316 82-83 -1.7324999363314397 2316 84-85 -1.6174540119204863 2316 86-87 -1.8703578860559418 2316 88-89 -1.7891950058434922 2316 90-91 -1.8377909514357889 2316 92-93 -1.548747656879783 2316 94-95 -1.6337284831131527 2316 96-97 -1.675550099529655 2316 98-99 -1.8022943484007428 2316 100-101 -1.8706332940091634 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 14.0 4 37.0 5 76.0 6 173.0 7 387.0 8 859.0 9 1654.0 10 2612.0 11 4166.0 12 5878.0 13 8259.0 14 10436.0 15 13921.0 16 18074.0 17 22777.0 18 19329.0 19 12336.0 20 11532.0 21 11578.0 22 12492.0 23 13967.0 24 16206.0 25 19188.0 26 23252.0 27 28728.0 28 35494.0 29 43480.0 30 53314.0 31 65198.0 32 80732.0 33 101430.0 34 132580.0 35 177319.0 36 237839.0 37 286196.0 38 152307.0 39 6744.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.678669347244195 19.45629347784076 15.271561849500337 21.59347532541471 2 16.22534813901641 21.341466032487862 38.682821666344665 23.750364162151055 3 19.457184246776137 25.637443284199062 30.055970629832114 24.84940183919269 4 13.04386223138063 17.39770385634383 36.609201746639194 32.949232165636346 5 13.559897899982706 38.04955113525554 33.02329631941949 15.367254645342268 6 31.275639150403876 36.44557125360995 17.067969774856905 15.210819821129268 7 28.47073628898869 33.99022059170756 19.905260065658062 17.63378305364569 8 27.597482841879124 33.15584927715417 19.2901373692251 19.956530511741608 9 27.53669318535854 16.26747571981935 19.940657987376024 36.25517310744609 10-11 25.61549255349683 26.61186558761263 26.07229768350092 21.700344175389617 12-13 27.066960432123466 24.141602309646576 27.209948775031776 21.58148848319818 14-15 23.608826577861187 25.124407401274436 26.54912819757993 24.717637823284452 16-17 22.07619169111185 28.69377787072705 26.90661685021763 22.32341358794347 18-19 21.914865301621703 26.037137956136785 30.60161820812244 21.44637853411907 20-21 23.083046470281232 24.92559345847983 30.624187016924765 21.367173054314172 22-23 22.40998857435836 25.089372068077935 30.666072590541926 21.834566767021784 24-25 22.379203632630382 25.39627635760607 30.81547743895633 21.40904257080722 26-27 22.09251691668338 25.722834410002875 30.687013911760324 21.49763476155342 28-29 21.63127309526365 25.794507735475857 31.008632453732808 21.565586715527687 30-31 21.89683701042473 25.444850988564564 30.874412620067144 21.78389938094356 32-33 21.61085099264946 25.66623562879145 30.657310783623178 22.065602594935914 34-35 22.510941992718422 25.479110852690358 30.368169841316572 21.64177731327465 36-37 22.681438319405046 25.25563137315035 30.178639883462395 21.884290423982215 38-39 22.20463298795124 25.44730134907246 30.398336866684065 21.949728796292238 40-41 22.239115089078016 25.539438847447194 30.40016877875203 21.82127728472276 42-43 22.292791089678783 25.799595660014653 29.68553412833701 22.22207912196956 44-45 22.327983919561007 25.76096139192084 29.565469373111263 22.345585315406893 46-47 22.747373078775897 26.174827981774456 28.78962153383927 22.288177405610373 48-49 23.008454176645376 26.313709135846388 28.255593954205775 22.422242733302465 50-51 22.811112051486795 26.548860080684232 28.0462652570844 22.59376261074457 52-53 23.170594837261504 26.658111166307886 27.815679564313445 22.355614432117164 54-55 22.61397402677052 26.445408687385964 28.00254519249935 22.938072093344168 56-57 22.87486699232465 26.848998341045476 27.497615827836704 22.778518838793165 58-59 23.208723410127227 26.23300042010236 27.65184247081515 22.906433698955265 60-61 22.921749444650924 26.41091170471007 28.29311882323647 22.374220027402536 62-63 22.936521419674 26.370771432093605 28.204886024850246 22.487821123382155 64-65 22.725342098379215 26.449760812504543 28.05383044996812 22.771066639148117 66-67 22.715221349766544 26.71876255351824 27.699769214587505 22.866246882127715 68-69 22.910701663735594 26.39244902308623 28.003072644114525 22.693776669063656 70-71 22.804892034621044 26.475573864092844 27.95349353507886 22.76604056620726 72-73 22.90374670663677 26.535890913015958 27.780162791496377 22.78019958885089 74-75 22.708375562834277 27.11595482422152 27.326169574458493 22.849500038485708 76-77 22.78240972005651 26.78618028359569 27.659733012455185 22.771676983892615 78-79 22.809222750038867 26.69573321010002 27.65469608770009 22.840347952161025 80-81 22.659073868785047 27.305216062018072 27.339254857044725 22.696455212152156 82-83 22.915943472485093 27.02067098518367 27.165933352877463 22.897452189453773 84-85 22.647526296695975 26.862940096493027 27.07238755691957 23.41714604989143 86-87 22.874586978020712 26.74444384817657 27.081699772310852 23.299269401491863 88-89 22.81500817581749 26.843852312719807 26.928646518868167 23.412492992594537 90-91 22.675387859598935 26.78190924937958 27.235122592706634 23.307580298314846 92-93 22.83508197374816 26.530424503350858 27.418528382250802 23.215965140650177 94-95 22.52106746525911 26.394518855921874 27.48072642392637 23.60368725489264 96-97 22.583293188404774 26.408197722180333 27.383359178387913 23.625149911026984 98-99 22.421061798841592 26.453312938971468 27.008566613268435 24.1170586489185 100-101 22.23308122191039 26.5458792907189 27.234105450120698 23.986934037250016 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 903.0 1 684.5 2 728.5 3 901.0 4 631.0 5 576.5 6 1005.5 7 1900.0 8 2940.0 9 3503.5 10 3097.5 11 2436.0 12 2343.0 13 2446.5 14 2618.0 15 2787.5 16 2911.5 17 3040.0 18 3150.5 19 3341.5 20 3699.0 21 4062.0 22 4559.0 23 5282.5 24 6031.0 25 7056.0 26 8610.5 27 10469.0 28 12136.0 29 14440.5 30 17564.5 31 20396.5 32 24256.0 33 27471.0 34 29579.0 35 31897.5 36 35095.5 37 38667.5 38 40612.5 39 41026.0 40 41568.5 41 45317.5 42 49105.5 43 51624.0 44 53442.5 45 55195.5 46 56266.0 47 58900.0 48 68638.0 49 69133.0 50 61218.0 51 56637.0 52 52546.0 53 52499.5 54 53420.0 55 51276.0 56 46938.5 57 41953.5 58 39236.0 59 36090.5 60 31405.0 61 27117.5 62 23324.5 63 18593.5 64 14180.5 65 11652.0 66 8760.0 67 6912.0 68 5173.5 69 3327.0 70 2555.0 71 2161.0 72 1980.0 73 1929.5 74 1649.0 75 1372.0 76 1054.0 77 643.5 78 392.5 79 267.5 80 208.5 81 177.0 82 147.0 83 89.0 84 39.5 85 25.5 86 13.0 87 3.0 88 3.0 89 2.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03507977889926768 2 0.006991424465937964 3 0.004354308220013995 4 5.519545631003656E-4 5 6.746111326782246E-4 6 3.6796970873357703E-4 7 0.0 8 0.0 9 0.0012878939805675196 10-11 3.0664142394464753E-4 12-13 0.001011916699017337 14-15 0.002391803106768251 16-17 0.003066414239446475 18-19 0.0 20-21 0.0 22-23 0.001594535404512167 24-25 9.199242718339426E-4 26-27 0.004783606213536502 28-29 0.005764858770159374 30-31 0.0014105505501453787 32-33 5.212904207059008E-4 34-35 0.009383227572706215 36-37 0.011897687249052324 38-39 0.00935256343031175 40-41 0.001962505113245744 42-43 6.439469902837598E-4 44-45 0.001011916699017337 46-47 3.066414239446475E-5 48-49 8.585959870450132E-4 50-51 9.199242718339426E-4 52-53 0.002391803106768251 54-55 0.0026984445307128985 56-57 0.0014718788349343081 58-59 8.585959870450132E-4 60-61 0.0038636819417025596 62-63 0.00502891935269222 64-65 0.009413891715100679 66-67 0.003066414239446475 68-69 0.0031584066666298697 70-71 0.0 72-73 0.0010425808414118015 74-75 0.005734194627764909 76-77 0.0026677803883184336 78-79 0.0035263763753634464 80-81 0.0046916137863531075 82-83 0.004354308220013995 84-85 0.005427553203820262 86-87 0.003465048090574517 88-89 0.009260571003128356 90-91 0.012940268090464127 92-93 0.001011916699017337 94-95 0.0020544975404291387 96-97 0.001134573268595196 98-99 0.003250399093813264 100-101 0.004476964789591854 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1630569.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.58869613741507 #Duplication Level Percentage of deduplicated Percentage of total 1 72.44368558469614 33.02613169195149 2 14.586654688804817 13.299731365386473 3 5.343853651828445 7.3085796100806855 4 2.4796434640488667 4.521748496466045 5 1.3621382513934945 3.1049053419963966 6 0.8079776525537269 2.210078861285627 7 0.5397292097164327 1.7223885656774736 8 0.37279070371851275 1.3596033691741127 9 0.2638487423082356 1.0825668125396355 >10 1.5222699817756131 13.153565939929933 >50 0.15889796017090943 5.057421491365386 >100 0.10905106679999621 9.080958885837376 >500 0.007161846225646475 2.225246270816309 >1k 0.0018918084369632195 1.2815402849175483 >5k 2.702583481376028E-4 0.9116104954194049 >10k+ 1.351291740688014E-4 0.6539225171561686 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10615 0.6509997430344867 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9130 0.5599272401229264 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5668 0.34760871818365247 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3008 0.18447548064509997 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2250 0.13798864077509138 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1765 0.10824442265246058 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.22656569577859E-4 0.0 22-23 0.0 0.0 0.0 2.45313139155718E-4 0.0 24-25 0.0 0.0 0.0 4.599621359169713E-4 0.0 26-27 0.0 0.0 0.0 7.052752750726893E-4 0.0 28-29 0.0 0.0 0.0 0.0017785202588789556 0.0 30-31 0.0 0.0 0.0 0.0032503990938132638 0.0 32-33 0.0 0.0 0.0 0.005580873915792585 0.0 34-35 0.0 0.0 0.0 0.008187326019322089 0.0 36-37 0.0 0.0 0.0 0.011713702394685537 0.0 38-39 0.0 0.0 0.0 0.01845981372146778 0.0 40-41 0.0 0.0 0.0 0.02529791747543342 0.0 42-43 0.0 0.0 0.0 0.03192137223263781 0.0 44-45 0.0 0.0 0.0 0.037655566860402716 0.0 46-47 0.0 0.0 0.0 0.04719211514508126 0.0 48-49 0.0 0.0 0.0 0.05439818860778048 0.0 50-51 0.0 0.0 0.0 0.06117496407695719 0.0 52-53 0.0 0.0 0.0 0.07644570698940062 0.0 54-55 0.0 0.0 0.0 0.0999651042059551 0.0 56-57 0.0 0.0 0.0 0.13578082252268994 0.0 58-59 0.0 0.0 0.0 0.18171570782959812 0.0 60-61 0.0 0.0 0.0 0.23675784342766237 0.0 62-63 0.0 0.0 0.0 0.2760692739773662 0.0 64-65 0.0 0.0 0.0 0.32280142698653047 0.0 66-67 0.0 0.0 0.0 0.40059635624128753 0.0 68-69 0.0 0.0 0.0 0.50500776109444 0.0 70-71 0.0 0.0 0.0 0.61043108264661 0.0 72-73 0.0 0.0 0.0 0.7231218059462678 0.0 74-75 0.0 0.0 0.0 0.8408721127410125 0.0 76-77 0.0 0.0 0.0 0.9500057955229125 0.0 78-79 0.0 0.0 0.0 1.0508908240007016 0.0 80-81 0.0 0.0 0.0 1.1582766506661173 0.0 82-83 0.0 0.0 0.0 1.297338536425015 0.0 84-85 0.0 0.0 0.0 1.5092584245131608 0.0 86-87 0.0 0.0 0.0 1.7767724027624712 0.0 88-89 6.13282847889295E-5 0.0 0.0 2.0715774677428556 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10205 0.0 29.055109 1 GTATCAA 14505 0.0 28.893608 1 GTACATG 10875 0.0 24.293858 1 GAGTACT 9685 0.0 23.370481 12-13 TACATGG 11165 0.0 23.314507 2 ATCAACG 18195 0.0 22.945698 3 TCAACGC 18240 0.0 22.915129 4 CAACGCA 18370 0.0 22.778818 5 TATCAAC 18500 0.0 22.672186 2 AGTACTT 9830 0.0 22.66333 12-13 ACATGGG 11135 0.0 22.43675 3 AACGCAG 18790 0.0 22.370771 6 AGGTAAG 575 0.0 22.304901 2 GGTAAGG 625 0.0 22.038519 3 GTAAGGT 625 0.0 22.038519 4 GTACTTT 10325 0.0 21.96786 14-15 TACCGAC 110 0.0028789986 21.589458 7 TATACCG 115 0.0037253865 20.650787 5 CATGGGA 6750 0.0 20.546768 4 ACGCAGA 20850 0.0 20.137737 7 >>END_MODULE