FastQCFastQC Report
Thu 26 May 2016
SRR936516_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936516_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1663410
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83520.5021011055602648No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64100.38535297972237753No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39100.23505930588369675No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT25920.15582448103594423No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17470.10502521927847014No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA132300.030.10231
GTACATG116850.027.9411031
GGTATCA90100.027.4807431
TACATGG120950.026.796682
ACATGGG119950.025.871133
GAGTACT73800.024.82467512-13
ATCAACG161100.024.6611423
TCAACGC161700.024.6585484
CAACGCA162150.024.5029245
AACGCAG164750.024.116236
TATCAAC164200.024.0797882
GTACTTT77300.023.51303514-15
CATGGGG40450.023.145324
ATGGGAG26200.022.6744795
AGTACTT74650.022.66395412-13
GTGTAGC9500.022.0112441
CATGGGA75700.021.9101374
ACGCAGA182200.021.6761077
CGCAGAG184400.021.3672338
GCAGAGT186400.020.8083749
Produced by FastQC (version 0.11.5)