##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936516_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1663410 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.386902808087 31.0 31.0 34.0 30.0 34.0 2 31.430795173769546 31.0 31.0 34.0 28.0 34.0 3 31.36592601944199 31.0 31.0 34.0 28.0 34.0 4 35.04465225049747 37.0 35.0 37.0 32.0 37.0 5 35.09095953493126 37.0 35.0 37.0 32.0 37.0 6 35.16720892624188 37.0 35.0 37.0 32.0 37.0 7 35.03137650969995 37.0 35.0 37.0 32.0 37.0 8 35.019318748835225 37.0 35.0 37.0 32.0 37.0 9 36.49431048268316 39.0 37.0 39.0 32.0 39.0 10-11 36.37349571061855 39.0 37.0 39.0 32.0 39.0 12-13 36.2676327544021 39.0 35.0 39.0 32.0 39.0 14-15 37.23599353136028 39.5 37.0 41.0 31.5 41.0 16-17 37.159776002308504 39.0 36.0 41.0 31.0 41.0 18-19 37.05173799604427 39.0 36.0 41.0 31.0 41.0 20-21 37.05539013231855 39.0 36.0 41.0 31.0 41.0 22-23 37.054577043543084 39.0 36.0 41.0 31.0 41.0 24-25 37.15300767700086 39.0 36.0 41.0 31.0 41.0 26-27 37.08109846640335 39.0 36.0 41.0 31.0 41.0 28-29 36.96291984537787 39.0 36.0 41.0 30.5 41.0 30-31 36.7373113062925 39.0 36.0 41.0 30.0 41.0 32-33 36.544809758267654 39.0 35.0 41.0 30.0 41.0 34-35 36.3389026157111 39.0 35.0 40.0 30.0 41.0 36-37 36.11225765145093 38.5 35.0 40.0 29.5 41.0 38-39 35.89912889786643 38.0 35.0 40.0 29.0 41.0 40-41 35.641376148995136 38.0 35.0 40.0 28.0 41.0 42-43 35.32540504145099 38.0 34.5 40.0 27.0 41.0 44-45 35.114127905928186 38.0 34.0 40.0 26.0 41.0 46-47 34.864155559964175 38.0 34.0 40.0 24.5 41.0 48-49 34.574177442723084 38.0 34.0 40.0 24.0 41.0 50-51 33.56233219711316 37.0 32.5 39.5 22.5 40.5 52-53 33.65059245766227 37.5 33.0 39.5 23.0 40.5 54-55 34.06562513150696 38.0 33.0 40.0 23.0 41.0 56-57 33.905075116778185 38.0 33.0 40.0 22.0 41.0 58-59 33.670157086947896 37.0 33.0 40.0 22.0 41.0 60-61 33.370424609687326 37.0 33.0 40.0 20.5 41.0 62-63 33.025451933077235 36.0 32.5 39.5 19.5 41.0 64-65 32.665246090861544 36.0 32.0 39.0 18.0 41.0 66-67 32.307277219687265 35.0 31.5 39.0 17.5 40.5 68-69 31.877301146440146 35.0 31.0 38.5 14.0 40.0 70-71 31.488376888440012 35.0 31.0 37.5 12.0 40.0 72-73 31.085279035234848 35.0 31.0 37.0 8.0 39.5 74-75 30.681090049957618 35.0 30.0 36.5 7.0 39.0 76-77 30.236201537804874 34.5 30.0 36.0 4.5 39.0 78-79 29.7768280219549 34.0 29.0 35.5 2.0 37.5 80-81 29.34409225626875 34.0 29.0 35.0 2.0 37.0 82-83 28.969339489362213 34.0 29.0 35.0 2.0 36.5 84-85 28.605086539097396 34.0 28.5 35.0 2.0 36.0 86-87 28.255419289291275 34.0 27.5 35.0 2.0 36.0 88-89 27.90653416776381 34.0 27.0 35.0 2.0 35.5 90-91 27.55448596557674 33.5 26.5 35.0 2.0 35.0 92-93 27.149253942203064 33.0 26.0 35.0 2.0 35.0 94-95 26.657897932560225 33.0 25.0 35.0 2.0 35.0 96-97 26.065076860184803 33.0 24.0 35.0 2.0 35.0 98-99 25.317809800349885 32.5 20.5 35.0 2.0 35.0 100-101 22.700281950932123 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7536512209245032 1101 2 0.6424644215752835 1101 3 0.7423078836362222 1101 4 0.7818598117813877 1101 5 0.7503931285214378 1101 6 0.6370267115434984 1101 7 0.6658122562416864 1101 8 0.6423094058275183 1101 9 0.8878042542404074 1101 10-11 0.904054935819893 1101 12-13 0.9826023334708509 1101 14-15 1.1405904081591416 1101 16-17 1.3195935991762227 1101 18-19 1.461087555907639 1101 20-21 1.3097670563970354 1101 22-23 1.2653588211865028 1101 24-25 1.1508185908330972 1101 26-27 1.1278214029274878 1101 28-29 1.0994949599297925 1101 30-31 0.9863606683113275 1101 32-33 0.8590597854014916 1101 34-35 1.0246860989251374 1101 36-37 1.0394468288653727 1101 38-39 0.9328844681241719 1101 40-41 1.0909635125702195 1101 42-43 1.2069933583693455 1101 44-45 1.158781956588527 1101 46-47 1.2469850178547475 1101 48-49 1.1525067304965049 1101 50-51 1.1779015187967445 1101 52-53 1.2616553665092738 1101 54-55 1.222360486817614 1101 56-57 1.377001393201077 1101 58-59 1.3898815223465064 1101 60-61 1.3060237206085148 1101 62-63 1.3982901983149887 1101 64-65 1.2022074858576843 1101 66-67 1.2277775915284863 1101 68-69 1.2207608348923067 1101 70-71 1.1021961993481888 1101 72-73 1.3607414578760313 1101 74-75 1.0497561011957721 1101 76-77 1.1515031398816014 1101 78-79 1.1536578641647601 1101 80-81 1.5582295395030243 1101 82-83 1.3596967562555555 1101 84-85 1.1572372482352584 1101 86-87 1.3559541479531987 1101 88-89 1.5720356002000955 1101 90-91 1.3581221284854514 1101 92-93 1.552655589461633 1101 94-95 1.588348916005117 1101 96-97 1.5727755310606106 1101 98-99 1.9177547934557424 1101 100-101 2.264743366360129 1102 1 0.3500980836711278 1102 2 0.5525976447338188 1102 3 0.6739400037475392 1102 4 0.37778204500376944 1102 5 0.45481701046810485 1102 6 0.36684172701734497 1102 7 0.5673705540410552 1102 8 0.4673723535750369 1102 9 0.4177159325092106 1102 10-11 0.6267336645482331 1102 12-13 0.5934792365196202 1102 14-15 0.7774699928209401 1102 16-17 0.4022679624880183 1102 18-19 0.5411598191422584 1102 20-21 0.7494182907965197 1102 22-23 0.4835875534227654 1102 24-25 0.7010668738991797 1102 26-27 0.6301701529372323 1102 28-29 0.5125514530971387 1102 30-31 0.46615424647176695 1102 32-33 0.5908189703158584 1102 34-35 0.47243401171035515 1102 36-37 0.5295961884743505 1102 38-39 0.48617957807810086 1102 40-41 0.6824910965460163 1102 42-43 0.5604015014302846 1102 44-45 0.8113778203563555 1102 46-47 0.9866654849543082 1102 48-49 1.0861983917713616 1102 50-51 1.032706595152554 1102 52-53 0.8980340975762289 1102 54-55 0.9528061490746893 1102 56-57 1.1169999120776097 1102 58-59 0.9282527542514671 1102 60-61 1.0804100287913343 1102 62-63 0.9604283949302328 1102 64-65 1.1229482814548462 1102 66-67 0.9358111272820224 1102 68-69 0.9970523355822003 1102 70-71 0.9947441994417296 1102 72-73 1.1620015821344936 1102 74-75 0.8944603052478151 1102 76-77 0.9096380156621144 1102 78-79 0.8631553735387101 1102 80-81 0.9526032191594034 1102 82-83 0.9019598349655773 1102 84-85 0.8359429801830913 1102 86-87 0.7663212327124356 1102 88-89 1.0458688256972444 1102 90-91 1.1246799195846826 1102 92-93 1.0612753382942763 1102 94-95 1.2620172690737732 1102 96-97 1.177945041744188 1102 98-99 1.2527899896266455 1102 100-101 1.762858441858448 1103 1 0.440575867645844 1103 2 0.47998935322982206 1103 3 0.5388293883915232 1103 4 0.5497694032487956 1103 5 0.49818566635209294 1103 6 0.5120211283930587 1103 7 0.5669057836376297 1103 8 0.5543959639115315 1103 9 0.48327458653411526 1103 10-11 0.6084644695305741 1103 12-13 0.4035052636622112 1103 14-15 0.8065497474221672 1103 16-17 0.99508818929597 1103 18-19 0.9538294603395059 1103 20-21 0.786953148576778 1103 22-23 0.735771974318844 1103 24-25 0.5619090378701799 1103 26-27 0.7104508742608928 1103 28-29 0.5273460245788257 1103 30-31 0.5095730975593327 1103 32-33 0.7563144155659032 1103 34-35 0.6201083006116406 1103 36-37 0.6354392819861587 1103 38-39 0.49535414584162396 1103 40-41 0.877505039658125 1103 42-43 0.7596578687848137 1103 44-45 0.7307903505664228 1103 46-47 0.9230198259098827 1103 48-49 0.6800578452013681 1103 50-51 0.6945600995213965 1103 52-53 0.6453198384202494 1103 54-55 0.7390229179908445 1103 56-57 0.8419041693745015 1103 58-59 0.5821047713550129 1103 60-61 0.49508190088651816 1103 62-63 0.38252915715369085 1103 64-65 0.5763132191756171 1103 66-67 0.5869247171686212 1103 68-69 0.5391698295401817 1103 70-71 0.5155603362701378 1103 72-73 0.5299417197065353 1103 74-75 0.486746975632645 1103 76-77 0.4655480361120148 1103 78-79 0.2916941353903546 1103 80-81 0.4448638624016432 1103 82-83 0.19915076264730303 1103 84-85 0.30018168626228814 1103 86-87 0.27851123085335416 1103 88-89 0.19093668217614734 1103 90-91 0.1748881367254107 1103 92-93 0.1790388631130142 1103 94-95 0.48956142226209565 1103 96-97 0.627909719193525 1103 98-99 0.8197262231272973 1103 100-101 1.4387963485325521 1104 1 0.18245328303935082 1104 2 0.2513289711010245 1104 3 0.3901271093435952 1104 4 0.5036878487286245 1104 5 0.3582376763206696 1104 6 0.4156560473080759 1104 7 0.3556658515395341 1104 8 0.33949005138941146 1104 9 0.4512154974309297 1104 10-11 0.40435821027531915 1104 12-13 0.4673189668442177 1104 14-15 0.5706498666116744 1104 16-17 0.6674456133578062 1104 18-19 0.7277937757221764 1104 20-21 0.8313241530192954 1104 22-23 0.6515555550607033 1104 24-25 0.6664631607437315 1104 26-27 0.39585052030415824 1104 28-29 0.3134969257237614 1104 30-31 0.2440080309215631 1104 32-33 0.2856711533850955 1104 34-35 0.3307714277109923 1104 36-37 0.41495891213749303 1104 38-39 0.3922172946118039 1104 40-41 0.43699360830044753 1104 42-43 0.539337863005926 1104 44-45 0.7111833147734572 1104 46-47 0.6165005208208498 1104 48-49 0.5150919767432498 1104 50-51 0.6268900738775898 1104 52-53 0.6735713644071808 1104 54-55 0.7448910149316461 1104 56-57 0.8200516754242742 1104 58-59 0.8984875502765348 1104 60-61 0.8199217174069062 1104 62-63 0.8565064750285103 1104 64-65 0.7808297792940806 1104 66-67 0.6415976683731586 1104 68-69 0.5600352176284566 1104 70-71 0.6544771564384497 1104 72-73 0.9942083430315591 1104 74-75 1.1027768813645729 1104 76-77 1.0529060019443541 1104 78-79 0.941053762652249 1104 80-81 1.0445890041649228 1104 82-83 0.9027799026769152 1104 84-85 0.9139437406560482 1104 86-87 0.8593321937601317 1104 88-89 0.8028823606534097 1104 90-91 0.9084251757690076 1104 92-93 0.9506338354827157 1104 94-95 1.0539569869675525 1104 96-97 0.8501501208260613 1104 98-99 0.5217813392467754 1104 100-101 1.2865855636876802 1105 1 0.35553496031061727 1105 2 0.3741169560248636 1105 3 0.43670010014404426 1105 4 0.4736915915278743 1105 5 0.44837422634366675 1105 6 0.32038799520720573 1105 7 0.6064893364309185 1105 8 0.5425907741225657 1105 9 0.3507544840024295 1105 10-11 0.47679622275224176 1105 12-13 0.4938883529560769 1105 14-15 0.736283235255307 1105 16-17 0.6070398027655486 1105 18-19 0.5344063766638172 1105 20-21 0.5135411607963078 1105 22-23 0.4619772483804354 1105 24-25 0.3924747178448911 1105 26-27 0.4668580514791927 1105 28-29 0.47400742420661857 1105 30-31 0.423267398623274 1105 32-33 0.4882256484041321 1105 34-35 0.4157018958923757 1105 36-37 0.5984127009966542 1105 38-39 0.3483826931923062 1105 40-41 0.41546353539182235 1105 42-43 0.2714735926515175 1105 44-45 0.4980795812276142 1105 46-47 0.8706321016218084 1105 48-49 0.9201305303377865 1105 50-51 0.8673414471810759 1105 52-53 1.0603765468720923 1105 54-55 0.9306374409726885 1105 56-57 0.874498010450381 1105 58-59 0.7965076647150298 1105 60-61 0.7268765565977446 1105 62-63 0.9707053571607105 1105 64-65 0.9722242413322917 1105 66-67 0.8301446335982661 1105 68-69 0.832145355366233 1105 70-71 1.0069480254446184 1105 72-73 0.7610084706458267 1105 74-75 0.7412797892848069 1105 76-77 0.7171741465793744 1105 78-79 0.7860562969831086 1105 80-81 0.8933357188572764 1105 82-83 0.9971233038179896 1105 84-85 0.8647787577886774 1105 86-87 0.8553390689094655 1105 88-89 0.9042334973733084 1105 90-91 0.9457975787704278 1105 92-93 0.8864127175320604 1105 94-95 0.7919997101233278 1105 96-97 0.9797528316623669 1105 98-99 1.20252056281619 1105 100-101 1.6082397980918763 1106 1 0.39431561390467706 1106 2 0.21831494621190473 1106 3 0.28615158273661834 1106 4 0.37073205382976226 1106 5 0.42362953546008697 1106 6 0.2968728161455161 1106 7 0.51220374267605 1106 8 0.5294097541924998 1106 9 0.6055767627294273 1106 10-11 0.6732978295547696 1106 12-13 0.5445107975685133 1106 14-15 0.6711475160657372 1106 16-17 0.43451095482560476 1106 18-19 0.683366462777272 1106 20-21 0.4096377159178317 1106 22-23 0.3234176619645339 1106 24-25 0.19492884249304154 1106 26-27 0.1502909070016898 1106 28-29 0.31143595500463306 1106 30-31 0.2688054682420855 1106 32-33 0.2589318998627732 1106 34-35 0.374294496095537 1106 36-37 0.02317518476022684 1106 38-39 0.22348364992532765 1106 40-41 0.1941008865593119 1106 42-43 0.14385509649348904 1106 44-45 0.056775332505608844 1106 46-47 0.07105715753500164 1106 48-49 -0.020440740182714023 1106 50-51 -0.020295324952350313 1106 52-53 0.0625986156226972 1106 54-55 0.1759068483098929 1106 56-57 0.28924647083893973 1106 58-59 0.1734076415128456 1106 60-61 0.21071616484139355 1106 62-63 0.18338895892952678 1106 64-65 -0.029949347773971624 1106 66-67 -0.06478687912312608 1106 68-69 0.05484893415058423 1106 70-71 0.06995863517225587 1106 72-73 0.25357203341318524 1106 74-75 0.26088512755705295 1106 76-77 0.1022205747692837 1106 78-79 0.19034250991659363 1106 80-81 0.49944099010853904 1106 82-83 0.484729865478311 1106 84-85 0.47459688937977873 1106 86-87 0.6363090564374403 1106 88-89 0.6474373316538937 1106 90-91 0.830965291123178 1106 92-93 0.6941912679600755 1106 94-95 0.7537581161534455 1106 96-97 0.9803597627886091 1106 98-99 1.4382265523146494 1106 100-101 1.8039982204519802 1107 1 0.30264516080136517 1107 2 0.3339701466059104 1107 3 0.38151356498387656 1107 4 0.41522734209979006 1107 5 0.4500883437418963 1107 6 0.3435824300999428 1107 7 0.44151299799848687 1107 8 0.4117138233570543 1107 9 0.5442220228230994 1107 10-11 0.6635620296785163 1107 12-13 0.5320206527151186 1107 14-15 0.4996740475341497 1107 16-17 0.48759624861254025 1107 18-19 0.7911924519578193 1107 20-21 0.6005793320237913 1107 22-23 0.6633263534969629 1107 24-25 0.48944237799030077 1107 26-27 0.38997579875782407 1107 28-29 0.5739770605827559 1107 30-31 0.40814787105012584 1107 32-33 0.6499602807123281 1107 34-35 0.7563467206462491 1107 36-37 0.7326760392565106 1107 38-39 0.7292236358141437 1107 40-41 0.7669980662284246 1107 42-43 0.7500036558831198 1107 44-45 0.7432587071445269 1107 46-47 0.8549075723552946 1107 48-49 0.7297992683918437 1107 50-51 0.7251783763546769 1107 52-53 0.7859218269505703 1107 54-55 0.7112103013428452 1107 56-57 0.8696414526213019 1107 58-59 0.9633453970973207 1107 60-61 0.9403915483126255 1107 62-63 0.9708633388361392 1107 64-65 0.9367818472254328 1107 66-67 0.810185092478271 1107 68-69 0.7777646240700271 1107 70-71 0.7666387576621112 1107 72-73 0.8138434469453273 1107 74-75 0.6357421909436489 1107 76-77 0.6753502010989472 1107 78-79 0.8167913561986637 1107 80-81 0.6913459283013985 1107 82-83 0.7431397001748721 1107 84-85 0.6975063325007085 1107 86-87 0.7738717792262193 1107 88-89 0.772981542877222 1107 90-91 0.6721489871105639 1107 92-93 0.955797490823489 1107 94-95 0.8860896383535817 1107 96-97 0.9983512000717383 1107 98-99 1.3018209667561962 1107 100-101 1.777987702158395 1108 1 0.13668562331219292 1108 2 0.1697648208582656 1108 3 0.2749221715321504 1108 4 0.4789911422254889 1108 5 0.3557182854249703 1108 6 0.2998016170603677 1108 7 0.44752174488369434 1108 8 0.4483954688795322 1108 9 0.13657326762743338 1108 10-11 0.2437082727907125 1108 12-13 0.2284152739164469 1108 14-15 0.5638893040149355 1108 16-17 0.5699716799787566 1108 18-19 0.5804250545547021 1108 20-21 0.5802330891729355 1108 22-23 0.5344848911181046 1108 24-25 0.46835753686243464 1108 26-27 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1.1305892696703808 2311 68-69 0.9633153545578033 2311 70-71 0.8498661854597067 2311 72-73 0.8109582806649591 2311 74-75 0.9072385189730241 2311 76-77 1.0550635399879802 2311 78-79 1.1495799704854939 2311 80-81 0.9757341936208093 2311 82-83 0.8431764849869765 2311 84-85 0.7801129759028775 2311 86-87 0.9702518932916888 2311 88-89 0.9883855898576108 2311 90-91 1.0639508569509246 2311 92-93 1.1854870660728416 2311 94-95 1.117840139668722 2311 96-97 1.1593332568961365 2311 98-99 1.3433432069187 2311 100-101 1.0774450447975283 2312 1 -0.03624273953772672 2312 2 0.04416542047158245 2312 3 0.11018902632050498 2312 4 0.1570816044296066 2312 5 0.11465139040905825 2312 6 0.042907079424999495 2312 7 0.012715934557270714 2312 8 0.09002627034475807 2312 9 0.06139753414611704 2312 10-11 0.03391305986967552 2312 12-13 -0.16818598912392702 2312 14-15 -0.029738854486872413 2312 16-17 -0.02271352237435309 2312 18-19 0.11300544250818945 2312 20-21 -0.05929002430872998 2312 22-23 -0.053336970264552974 2312 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-1.8299311626489931 2315 84-85 -1.9929270522056264 2315 86-87 -2.3094463379936094 2315 88-89 -2.418530164166416 2315 90-91 -2.1786096691555166 2315 92-93 -2.2051323818336 2315 94-95 -2.473628861418497 2315 96-97 -2.4261378258118285 2315 98-99 -2.546150906204794 2315 100-101 -2.8587807911654544 2316 1 -5.278895591127938 2316 2 -4.965364182123714 2316 3 -5.855889897490552 2316 4 -7.71543144241717 2316 5 -8.321144848094697 2316 6 -9.036348615558076 2316 7 -11.264513055920744 2316 8 -11.377856796248203 2316 9 -11.148759709918782 2316 10-11 -11.209803089783502 2316 12-13 -9.897461379513732 2316 14-15 -9.71085335335303 2316 16-17 -8.818034902082935 2316 18-19 -9.095442327304376 2316 20-21 -10.438322867396913 2316 22-23 -9.970112451333023 2316 24-25 -9.820798267744102 2316 26-27 -7.951838296516648 2316 28-29 -5.9937051851933205 2316 30-31 -8.16832800650456 2316 32-33 -5.925315840482959 2316 34-35 -4.3873958102645645 2316 36-37 -5.009666426259088 2316 38-39 -4.182629175746349 2316 40-41 -3.6217526535530595 2316 42-43 -3.6510773907876555 2316 44-45 -3.935602649198664 2316 46-47 -3.9421375619219283 2316 48-49 -4.710125382423719 2316 50-51 -4.675038209146678 2316 52-53 -4.584871610957244 2316 54-55 -4.110860802939385 2316 56-57 -4.523747698137829 2316 58-59 -4.478891235222388 2316 60-61 -4.767429257206032 2316 62-63 -4.706181202254342 2316 64-65 -5.228578723507024 2316 66-67 -4.917795743249513 2316 68-69 -5.465980511075276 2316 70-71 -4.942252677589387 2316 72-73 -3.779513295562431 2316 74-75 -3.9966697497608266 2316 76-77 -4.523327932880235 2316 78-79 -4.843314086362053 2316 80-81 -5.5450668399709215 2316 82-83 -4.88534418684765 2316 84-85 -4.901508471125542 2316 86-87 -5.525613739783246 2316 88-89 -6.852441283527401 2316 90-91 -6.1958036650129 2316 92-93 -5.282342391291497 2316 94-95 -4.917915674284771 2316 96-97 -6.267087931734359 2316 98-99 -7.999970746569673 2316 100-101 -8.330080794995755 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3639.0 3 1396.0 4 1424.0 5 1595.0 6 1812.0 7 2114.0 8 2772.0 9 3407.0 10 4370.0 11 5700.0 12 7414.0 13 9123.0 14 10466.0 15 13021.0 16 16187.0 17 20080.0 18 15938.0 19 11607.0 20 11634.0 21 12469.0 22 13504.0 23 15320.0 24 17728.0 25 20155.0 26 23368.0 27 27743.0 28 33394.0 29 39636.0 30 48507.0 31 58927.0 32 73869.0 33 94745.0 34 124601.0 35 169138.0 36 227793.0 37 282839.0 38 208331.0 39 27643.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.96668884602751 18.661706190448115 14.336610504353853 22.034994459170523 2 15.717983133445907 20.5434244011796 39.68667465254225 24.051917812832244 3 19.397749587833296 25.62239878015491 29.312538405759632 25.667313226252165 4 12.586977802471095 16.550916739423762 37.02656776511844 33.8355376929867 5 13.768820107493646 38.13228285092683 32.73108187581104 15.36781516576848 6 31.328878901060968 35.869465209544934 17.70447401637969 15.097181873014403 7 28.97429881240299 33.44060210085551 19.997292711980652 17.587806374760856 8 27.428051063174557 32.95744895847869 19.676127532828733 19.93837244551802 9 27.896692596587503 16.43071862436466 20.039511496413706 35.633077282634126 10-11 25.581654145378874 26.39912946998545 26.311847888233856 21.707368496401816 12-13 26.9775406922723 24.000036084039728 27.090904717086094 21.931518506601876 14-15 23.67275844969129 25.09040540414561 26.193824976340473 25.04301116982262 16-17 22.36621800734629 28.641002266805778 26.448603780576086 22.544175945271842 18-19 22.21807856829004 26.41202442924592 29.631693659397463 21.738203343066576 20-21 23.546742788768682 25.37045638687585 29.38343201848336 21.699368805872105 22-23 22.79518663397471 25.54160589032481 29.462356346372843 22.200851129327646 24-25 22.656642960330593 25.842552046456603 29.540530017753447 21.960274975459356 26-27 22.339949916371946 26.137141589224655 29.495612947180085 22.027295547223318 28-29 21.898955368933535 26.347873477926154 29.665886998520126 22.08728415462019 30-31 22.18080754217434 25.916755691529303 29.636562135346246 22.265874630950112 32-33 21.816650313122278 26.194740652025434 29.413623223956982 22.57498581089531 34-35 22.802128510905145 26.12491308319933 29.078896856324914 21.99406154957061 36-37 22.909780430001767 25.779184316875302 28.91038042687553 22.400654826247397 38-39 22.455656939847113 25.97358129713841 29.13482520854458 22.435936554469897 40-41 22.540534431045025 26.000713023622968 29.20697464557736 22.25177789975465 42-43 22.483534981588637 26.24564514916961 28.584384158713462 22.686435710528293 44-45 22.384954657588064 26.412913081261046 28.503452419850085 22.698679841300805 46-47 22.73658005495969 26.639908236198007 27.905778582554657 22.717733126287648 48-49 22.9984342230918 26.72666056066777 27.46818773571946 22.806717480520973 50-51 22.778497356762117 26.961026406528095 27.42790274471706 22.832573491992733 52-53 23.171508836983374 26.993393679358093 27.14777561955673 22.68732186410181 54-55 22.62954997362334 26.819245492296663 27.35107708446993 23.20012744961006 56-57 22.86251326014166 27.214545109130704 26.92200286229683 23.00093876843081 58-59 23.174125554606995 26.5884004472928 27.053849483569202 23.183624514531004 60-61 22.876164160538874 26.726859425379196 27.717106058173908 22.679870355908022 62-63 22.83658037156073 26.86655099731748 27.615696402827528 22.681172228294262 64-65 22.62971102251744 26.98843542997338 27.396725801588858 22.985127745920327 66-67 22.630338306675117 27.207223509410234 27.028974354966508 23.133463828948145 68-69 22.785903319942623 26.905501093581474 27.37470706565457 22.933888520821334 70-71 22.676765870159024 26.938517120861288 27.2959250387242 23.088791970255485 72-73 22.754380479357327 26.972658833716164 27.214983223696077 23.05797746323043 74-75 22.499760236258233 27.552236176455292 26.918658609803224 23.029344977483255 76-77 22.644135410030167 27.187989610302143 27.07412867218062 23.09374630748707 78-79 22.75911810536905 27.210441359202225 27.13034892820754 22.90009160722119 80-81 22.623832591888014 27.793286768282456 26.808313887804964 22.77456675202457 82-83 22.74861685386672 27.58031716544096 26.697568578647317 22.973497402045 84-85 22.655182167724224 27.362447985037363 26.580047957622703 23.402321889615703 86-87 22.80738194612484 27.195308355113752 26.678650280377486 23.318659418383923 88-89 22.746962890375485 27.251398881495987 26.483806340393745 23.51783188773478 90-91 22.600676245203818 27.26597454942037 26.83643739982276 23.29691180555305 92-93 22.85507458989387 27.11811008472681 26.87803476131955 23.148780564059766 94-95 22.503941955179705 26.945026071768385 27.00265602852337 23.548375944528534 96-97 22.532801632979453 26.94022279106611 26.982400076959266 23.544575498995172 98-99 22.36116815200143 27.076457108290438 26.62475338792823 23.937621351779907 100-101 22.40947990120844 27.039364799718594 26.84145096724636 23.709704331826607 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 668.0 1 497.5 2 448.5 3 644.5 4 635.0 5 595.0 6 892.0 7 1591.0 8 2463.5 9 2893.5 10 2575.5 11 2212.0 12 2184.5 13 2294.5 14 2481.0 15 2568.5 16 2596.5 17 2714.5 18 2768.0 19 2911.5 20 3232.0 21 3629.0 22 4147.0 23 4860.0 24 5732.0 25 6771.5 26 8233.0 27 10168.5 28 12049.5 29 14091.5 30 17207.5 31 20542.5 32 24675.5 33 27656.5 34 29828.5 35 32411.5 36 35657.5 37 39289.5 38 41067.5 39 41664.5 40 42434.0 41 46236.0 42 49793.0 43 52259.0 44 54656.5 45 56843.5 46 57956.5 47 60172.5 48 69354.0 49 70695.5 50 63330.0 51 58476.5 52 54904.0 53 54902.5 54 55591.5 55 53427.0 56 48859.5 57 43747.5 58 40991.0 59 37663.5 60 32655.5 61 28338.0 62 24717.5 63 19486.5 64 14651.0 65 12125.5 66 9111.0 67 7126.5 68 5379.0 69 3502.5 70 2762.0 71 2336.0 72 1992.5 73 1986.0 74 1689.0 75 1315.0 76 983.0 77 588.0 78 367.5 79 247.5 80 165.5 81 126.0 82 89.0 83 51.5 84 35.5 85 21.5 86 9.0 87 9.0 88 9.5 89 7.0 90 3.0 91 1.5 92 2.0 93 3.0 94 2.5 95 2.0 96 2.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07238143332070866 2 0.028495680559813875 3 0.014909132444797132 4 0.020560174581131532 5 0.025730276961182148 6 0.02987838235912974 7 0.07394448752863095 8 0.05061890934886769 9 0.06667027371483879 10-11 0.058584474062317766 12-13 0.037573418459670195 14-15 0.045719335581726694 16-17 0.05642024515904076 18-19 0.05377507649947998 20-21 0.01758435983912565 22-23 0.012504433663378242 24-25 0.02119140801125399 26-27 0.02353598932313741 28-29 0.005741218340637606 30-31 0.0010219969821030294 32-33 0.009739030064746515 34-35 0.010911320720688225 36-37 0.0040879879284121175 38-39 0.009408383982301417 40-41 0.004268340337018534 42-43 0.002104111433741531 44-45 6.913508996579317E-4 46-47 5.410572258192508E-4 48-49 0.0011121731864062378 50-51 8.717033082643485E-4 52-53 1.8035240860641693E-4 54-55 4.508810215160423E-4 56-57 0.003637106906896075 58-59 0.004208222867483062 60-61 0.005230219849586091 62-63 0.002885638537702671 64-65 6.312334301224593E-4 66-67 4.8093975628377855E-4 68-69 0.0040879879284121175 70-71 0.002675227394328518 72-73 0.02104111433741531 74-75 0.018906944168906044 76-77 0.025038926061524216 78-79 0.019928941151009072 80-81 0.013045490889197493 82-83 0.004959691236676465 84-85 0.003997811724108909 86-87 0.0016532304122254887 88-89 0.0016532304122254887 90-91 0.00649268670983101 92-93 0.010941379455455962 94-95 0.012774962276287867 96-97 0.01157261288557842 98-99 0.02242381613673117 100-101 0.018786709229835098 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1663410.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.11609255409588 #Duplication Level Percentage of deduplicated Percentage of total 1 74.11251416519062 35.660045909890535 2 13.872408346822612 13.34972167927858 3 5.0451082528371 7.282526869168285 4 2.291516268708994 4.410352354976736 5 1.243932693884627 2.992659031500926 6 0.7391277649818915 2.1338363969502434 7 0.48933350866910846 1.648137147506031 8 0.34172752370796783 1.3154074527211659 9 0.254918545580696 1.103911589962465 >10 1.3566852628883934 12.3703044240824 >50 0.14699481176168383 4.971380790525657 >100 0.09838871273594389 8.760942535459188 >500 0.005571418243740378 1.8849319340388526 >1k 0.001519477702838285 1.216450675383662 >5k 2.5324628380638084E-4 0.8993912085552247 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8352 0.5021011055602648 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6410 0.38535297972237753 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3910 0.23505930588369675 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2592 0.15582448103594423 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1747 0.10502521927847014 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 1.2023493907094462E-4 0.0 24-25 0.0 0.0 0.0 4.2082228674830615E-4 0.0 26-27 0.0 0.0 0.0 8.115858387288761E-4 0.0 28-29 0.0 0.0 0.0 0.0013827017993158631 0.0 30-31 0.0 0.0 0.0 0.0027654035986317263 0.0 32-33 0.0 0.0 0.0 0.004568927684695896 0.0 34-35 0.0 0.0 0.0 0.00688345026181158 0.0 36-37 0.0 0.0 0.0 0.010640792107778598 0.0 38-39 0.0 0.0 0.0 0.016712656530861302 0.0 40-41 0.0 0.0 0.0 0.024197281488027605 0.0 42-43 0.0 0.0 0.0 0.030599791993555406 0.0 44-45 0.0 0.0 0.0 0.037032361233850945 0.0 46-47 0.0 0.0 0.0 0.04550892443835254 0.0 48-49 0.0 0.0 0.0 0.05368490029517678 0.0 50-51 0.0 0.0 0.0 0.0616805237433946 0.0 52-53 0.0 0.0 0.0 0.0768301260663336 0.0 54-55 0.0 0.0 0.0 0.10045629159377423 0.0 56-57 0.0 0.0 0.0 0.13652677331505764 0.0 58-59 0.0 0.0 0.0 0.1826669311835326 0.0 60-61 0.0 3.0058734767736156E-5 0.0 0.2343679549840388 0.0 62-63 0.0 6.011746953547231E-5 0.0 0.27431601349036017 0.0 64-65 0.0 6.011746953547231E-5 0.0 0.32123769846279626 0.0 66-67 0.0 6.011746953547231E-5 0.0 0.3967151814645818 0.0 68-69 0.0 6.011746953547231E-5 0.0 0.5055278013237867 0.0 70-71 0.0 6.011746953547231E-5 0.0 0.6124767796273919 0.0 72-73 0.0 6.011746953547231E-5 0.0 0.7299463150997048 0.0 74-75 0.0 6.011746953547231E-5 0.0 0.844770681912457 0.0 76-77 0.0 6.011746953547231E-5 0.0 0.9535231843021263 0.0 78-79 0.0 6.011746953547231E-5 0.0 1.0609531023620153 0.0 80-81 0.0 6.011746953547231E-5 0.0 1.1732525354542775 0.0 82-83 0.0 6.011746953547231E-5 0.0 1.320540335816185 0.0 84-85 0.0 6.011746953547231E-5 0.0 1.5321237698462795 0.0 86-87 0.0 6.011746953547231E-5 0.0 1.8006384475264667 0.0 88-89 0.0 6.011746953547231E-5 0.0 2.1038709638633892 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 13230 0.0 30.1023 1 GTACATG 11685 0.0 27.941103 1 GGTATCA 9010 0.0 27.480743 1 TACATGG 12095 0.0 26.79668 2 ACATGGG 11995 0.0 25.87113 3 GAGTACT 7380 0.0 24.824675 12-13 ATCAACG 16110 0.0 24.661142 3 TCAACGC 16170 0.0 24.658548 4 CAACGCA 16215 0.0 24.502924 5 AACGCAG 16475 0.0 24.11623 6 TATCAAC 16420 0.0 24.079788 2 GTACTTT 7730 0.0 23.513035 14-15 CATGGGG 4045 0.0 23.14532 4 ATGGGAG 2620 0.0 22.674479 5 AGTACTT 7465 0.0 22.663954 12-13 GTGTAGC 950 0.0 22.011244 1 CATGGGA 7570 0.0 21.910137 4 ACGCAGA 18220 0.0 21.676107 7 CGCAGAG 18440 0.0 21.367233 8 GCAGAGT 18640 0.0 20.808374 9 >>END_MODULE