FastQCFastQC Report
Thu 26 May 2016
SRR936516_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936516_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1663410
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105170.6322554271045623No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88560.5324003102061428No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55530.3338323083304777No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT30100.18095358330177166No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22310.13412207453363875No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18030.10839179757245658No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA108250.028.3524151
GTATCAA153350.027.7593731
CGGATTA700.00840121127.1371699
GTACATG109100.024.1687221
GAGTACT100250.023.37859712-13
TACATGG112600.023.0294192
ATCAACG188600.022.5111893
ACATGGG109600.022.4884453
TCAACGC189400.022.4405064
CAACGCA191000.022.3022485
GTACTTT105150.022.1529914-15
TATCAAC192450.022.0621762
AACGCAG196550.021.6724996
AGTACTT102650.021.60596312-13
TATATCG1100.002881904821.5857374
GTAAGGT6000.021.369884
ACTTTTT118250.020.00003816-17
GATGTGC9300.019.9151828
ACGCAGA215550.019.7627357
CGCAGAG217100.019.6216378
Produced by FastQC (version 0.11.5)