##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936516_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1663410 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.811782422854257 31.0 31.0 34.0 30.0 34.0 2 31.95621043519036 31.0 31.0 34.0 30.0 34.0 3 32.026895954695476 33.0 31.0 34.0 30.0 34.0 4 35.75878466523587 37.0 35.0 37.0 35.0 37.0 5 35.588552431451056 37.0 35.0 37.0 33.0 37.0 6 35.690850722311396 37.0 35.0 37.0 33.0 37.0 7 35.629206870224415 37.0 35.0 37.0 33.0 37.0 8 35.597353027816354 37.0 35.0 37.0 33.0 37.0 9 37.208720640130814 39.0 37.0 39.0 33.0 39.0 10-11 37.09182913412809 39.0 37.0 39.0 33.0 39.0 12-13 36.9747380381265 39.0 37.0 39.0 33.0 39.0 14-15 38.04725052753079 40.0 37.0 41.0 33.0 41.0 16-17 37.9505112990784 40.0 37.0 41.0 33.0 41.0 18-19 38.129938199241316 40.0 37.0 41.0 33.0 41.0 20-21 38.15633578011435 40.0 37.0 41.0 33.0 41.0 22-23 38.06660534684774 40.0 37.0 41.0 33.0 41.0 24-25 37.918615975616355 40.0 37.0 41.0 33.0 41.0 26-27 37.70142027521777 39.5 37.0 41.0 32.5 41.0 28-29 37.59280604300804 39.0 36.5 41.0 32.0 41.0 30-31 37.509913370726395 39.0 36.5 41.0 32.0 41.0 32-33 37.29461377531697 39.0 36.0 41.0 32.0 41.0 34-35 37.09226799165569 39.0 35.5 41.0 31.0 41.0 36-37 36.85325115275248 39.0 35.0 41.0 31.0 41.0 38-39 36.65181494640528 39.0 35.0 41.0 30.5 41.0 40-41 36.40639048701162 39.0 35.0 40.0 30.0 41.0 42-43 36.06456135288353 38.5 35.0 40.0 30.0 41.0 44-45 35.70949555431312 38.0 35.0 40.0 28.5 41.0 46-47 35.2343021263549 38.0 34.5 40.0 26.5 41.0 48-49 34.7702731136641 38.0 34.0 40.0 25.0 41.0 50-51 34.43352661099789 38.0 34.0 40.0 24.0 41.0 52-53 34.04593395494797 38.0 33.0 40.0 23.0 41.0 54-55 33.82123349023993 37.5 33.0 40.0 23.0 41.0 56-57 33.50330315436362 37.0 33.0 40.0 22.0 41.0 58-59 33.11058638579785 36.5 32.0 39.5 20.5 41.0 60-61 32.68232726748066 36.0 31.5 39.0 19.0 40.5 62-63 32.3480455810654 35.5 31.0 39.0 18.0 40.0 64-65 31.91474350881623 35.0 31.0 38.5 17.0 40.0 66-67 31.490688705731 35.0 30.5 38.0 13.5 40.0 68-69 31.135820994222712 35.0 30.0 37.5 12.5 40.0 70-71 30.761024040976068 34.5 30.0 37.0 8.0 39.0 72-73 30.312821553315178 34.0 29.0 36.5 7.0 39.0 74-75 29.81737004106023 34.0 29.0 36.0 6.5 39.0 76-77 28.359399967536568 32.5 27.0 34.5 2.0 36.5 78-79 29.11963767201111 33.5 29.0 35.0 2.0 37.0 80-81 28.917613516811848 34.0 29.0 35.0 2.0 37.0 82-83 28.617215539163524 34.0 28.5 35.0 2.0 36.0 84-85 28.23617418435623 33.0 27.5 35.0 2.0 36.0 86-87 27.831381920272214 33.0 27.0 35.0 2.0 35.5 88-89 27.349797704715016 33.0 26.0 35.0 2.0 35.0 90-91 26.867312027702127 32.5 25.5 34.5 2.0 35.0 92-93 26.235037964182013 32.0 24.5 34.0 2.0 35.0 94-95 25.663138071792282 32.0 23.5 34.0 2.0 35.0 96-97 24.906715421934457 31.5 19.0 34.0 2.0 35.0 98-99 23.97148478126259 31.0 5.0 34.0 2.0 35.0 100-101 20.737451079409166 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.455956191580821 1101 2 0.3742635072002045 1101 3 0.4211437237591653 1101 4 0.3421186054752994 1101 5 0.3618957947834076 1101 6 0.4057626007827366 1101 7 0.378326633619551 1101 8 0.4021387729588426 1101 9 0.3905295575122736 1101 10-11 0.4573142658903109 1101 12-13 0.5496642242804626 1101 14-15 0.7363677190918665 1101 16-17 0.7423468415167775 1101 18-19 0.6735033908741173 1101 20-21 0.6286646458881009 1101 22-23 0.5302758324412835 1101 24-25 0.5971279468365722 1101 26-27 0.6677847455283867 1101 28-29 0.6945530768479813 1101 30-31 0.763511380148671 1101 32-33 0.8921634746727278 1101 34-35 0.9133614340302003 1101 36-37 0.6874557701920239 1101 38-39 0.6335730237791353 1101 40-41 0.7283090310243594 1101 42-43 0.6574197200067573 1101 44-45 0.7172193422931201 1101 46-47 0.6530068708576735 1101 48-49 0.5951592531969467 1101 50-51 0.5678938668295359 1101 52-53 0.7878334485609315 1101 54-55 0.6911167805703968 1101 56-57 0.6654533174397059 1101 58-59 0.5032657654966997 1101 60-61 0.6499757665216634 1101 62-63 0.6145259886714101 1101 64-65 0.7622255643166902 1101 66-67 0.717202773745484 1101 68-69 0.6295648036250014 1101 70-71 0.6895880319050249 1101 72-73 0.7989295141261685 1101 74-75 0.5644134647932226 1101 76-77 0.47475968548247494 1101 78-79 0.5440526328614084 1101 80-81 0.48283033349397897 1101 82-83 0.39691627768748816 1101 84-85 0.524419285959528 1101 86-87 0.4895898967068497 1101 88-89 0.5462691805452273 1101 90-91 0.5744808253785969 1101 92-93 0.5269804108765612 1101 94-95 0.575450277797227 1101 96-97 0.6730446758129496 1101 98-99 0.499773737157291 1101 100-101 1.2355907444045542 1102 1 0.3018070970149829 1102 2 0.31749282409046486 1102 3 0.3007166924762785 1102 4 0.2976529402559791 1102 5 0.22685397253381012 1102 6 0.2667965808733186 1102 7 0.3309564073194764 1102 8 0.2947768097686563 1102 9 0.3907400329529125 1102 10-11 0.3666098747489954 1102 12-13 0.3816896216502954 1102 14-15 0.3724377528458973 1102 16-17 0.33454015151592387 1102 18-19 0.34497305512680754 1102 20-21 0.3833142042794435 1102 22-23 0.4257650319330182 1102 24-25 0.40448250440940114 1102 26-27 0.40936611767235576 1102 28-29 0.45322427523277753 1102 30-31 0.5242401708503337 1102 32-33 0.6062778018139312 1102 34-35 0.5867312237886182 1102 36-37 0.4838800263172587 1102 38-39 0.34026302463662716 1102 40-41 0.5343110890064438 1102 42-43 0.6874592426172796 1102 44-45 0.6185192230237107 1102 46-47 0.43662837365231866 1102 48-49 0.6196960123428781 1102 50-51 0.48820942408275414 1102 52-53 0.5430208886338974 1102 54-55 0.47232366233777157 1102 56-57 0.3522670310839757 1102 58-59 0.3654246198866744 1102 60-61 0.5303929756173247 1102 62-63 0.4588844673237418 1102 64-65 0.2810537617894333 1102 66-67 0.2454236326411916 1102 68-69 0.4079575507972244 1102 70-71 0.27068850664407407 1102 72-73 0.4119770425988172 1102 74-75 0.3664956125781629 1102 76-77 0.328698304607677 1102 78-79 0.508245693408174 1102 80-81 0.48922021196389665 1102 82-83 0.372076667768642 1102 84-85 0.4085856862838142 1102 86-87 0.4921004009904131 1102 88-89 0.41307408540253476 1102 90-91 0.37935995790796895 1102 92-93 0.581198494614604 1102 94-95 0.506550752536274 1102 96-97 0.8025552814365433 1102 98-99 0.7646360316514098 1102 100-101 1.3221386353626876 1103 1 0.14279612843346356 1103 2 0.21174454374096285 1103 3 0.14336774285738585 1103 4 0.15033931318775018 1103 5 0.27396310062453466 1103 6 0.28971162084357616 1103 7 0.1939086289220029 1103 8 0.14213877295884458 1103 9 0.2592100087848479 1103 10-11 0.27023136573244955 1103 12-13 0.37011126136027883 1103 14-15 0.3257655089343814 1103 16-17 0.3267505995545861 1103 18-19 0.2378936723419045 1103 20-21 0.28950866421623545 1103 22-23 0.17576503193301818 1103 24-25 0.27770243376429704 1103 26-27 0.23851094013006247 1103 28-29 0.43957675710673527 1103 30-31 0.4310555640460976 1103 32-33 0.17766653577935188 1103 34-35 0.365948550429259 1103 36-37 0.29028084431316614 1103 38-39 0.33133013748287965 1103 40-41 0.36447282910683754 1103 42-43 0.4392086384157494 1103 44-45 0.4397629486232617 1103 46-47 0.38180591594642266 1103 48-49 0.42099922855407357 1103 50-51 0.3360398758395817 1103 52-53 0.34446353596612056 1103 54-55 0.31319371253297845 1103 56-57 0.19216849975844497 1103 58-59 0.5246103421398729 1103 60-61 0.4870113061620316 1103 62-63 0.4655771611330053 1103 64-65 0.2902822429197549 1103 66-67 0.28780883435154436 1103 68-69 0.4254013135784085 1103 70-71 0.4195526077781153 1103 72-73 0.3849720230784577 1103 74-75 0.39075476855511226 1103 76-77 0.2537847519027139 1103 78-79 0.2358084374126399 1103 80-81 0.35139719653351875 1103 82-83 0.44839754525516184 1103 84-85 0.5486674858748195 1103 86-87 0.458475191843732 1103 88-89 0.46180805082361687 1103 90-91 0.5087278701593156 1103 92-93 0.5826355647019845 1103 94-95 0.2978390960945596 1103 96-97 0.7499469899325462 1103 98-99 0.530586951896808 1103 100-101 0.6703910986458261 1104 1 0.17863460858660218 1104 2 0.21370069644800793 1104 3 0.10947602472252527 1104 4 0.10184724109615217 1104 5 0.20179565742014205 1104 6 0.17200728306772106 1104 7 0.12660050597621364 1104 8 0.10833662754763651 1104 9 0.09404224051815646 1104 10-11 0.17164033022842062 1104 12-13 0.24935928389793105 1104 14-15 0.2768346027329187 1104 16-17 0.26746826488488296 1104 18-19 0.10109084068806595 1104 20-21 0.18523915144851344 1104 22-23 0.2204610986791451 1104 24-25 0.1142267953780518 1104 26-27 0.21283065696467673 1104 28-29 0.29235170563853785 1104 30-31 0.26869127755785627 1104 32-33 0.16187934323411213 1104 34-35 0.15580191121230058 1104 36-37 0.19713821419794897 1104 38-39 0.17841902396389742 1104 40-41 0.2904734792314443 1104 42-43 0.18568785248733377 1104 44-45 0.1853399342121662 1104 46-47 0.2846635747754789 1104 48-49 0.3887429349866949 1104 50-51 0.24131346994511915 1104 52-53 0.18796301225462742 1104 54-55 0.1330777736446862 1104 56-57 0.10948384631098662 1104 58-59 0.1083778142386933 1104 60-61 0.1262264612889865 1104 62-63 0.11252157547908581 1104 64-65 0.06355959330079841 1104 66-67 0.13943161760722234 1104 68-69 0.08935045938691033 1104 70-71 -0.07990634407654795 1104 72-73 0.08012967829570883 1104 74-75 -0.045443447214022825 1104 76-77 -0.0527424991537373 1104 78-79 0.020789475444928485 1104 80-81 0.2881599371699224 1104 82-83 0.09205919151513697 1104 84-85 0.2653937472971428 1104 86-87 0.4475498478194595 1104 88-89 0.45156425409590994 1104 90-91 0.5467597985011352 1104 92-93 0.402885501495426 1104 94-95 0.333965436976559 1104 96-97 0.848962412332952 1104 98-99 0.9736739602400739 1104 100-101 1.8435806822048946 1105 1 0.0843291878807726 1105 2 0.17593640441022274 1105 3 0.05923136166422438 1105 4 0.13130072334962506 1105 5 0.14163050467664107 1105 6 0.09755389058619102 1105 7 0.01530263854312608 1105 8 0.10607851803415969 1105 9 0.12844699042044283 1105 10-11 0.17957755064062297 1105 12-13 0.27589552344813484 1105 14-15 0.25578411913839716 1105 16-17 0.1638603997582493 1105 18-19 0.17871076563871924 1105 20-21 0.29279283852010707 1105 22-23 0.1152204201137792 1105 24-25 0.28362609681426676 1105 26-27 0.22740029720018384 1105 28-29 0.17200275521059893 1105 30-31 0.2741694374264796 1105 32-33 0.2114914594072843 1105 34-35 0.2531861641095148 1105 36-37 0.2670170358929269 1105 38-39 0.15912254592419117 1105 40-41 0.309039351807769 1105 42-43 0.3178504446589443 1105 44-45 0.327238983596402 1105 46-47 0.37029357351867276 1105 48-49 0.30447090944174704 1105 50-51 0.3383931888070393 1105 52-53 0.35497092737733027 1105 54-55 0.2738826412172699 1105 56-57 0.02881374713370377 1105 58-59 0.08868703022583446 1105 60-61 0.1773322443972276 1105 62-63 0.09799578538244447 1105 64-65 0.24837101008152374 1105 66-67 0.13840787223911022 1105 68-69 0.27716647387196147 1105 70-71 0.21848901706214718 1105 72-73 0.20909190088315555 1105 74-75 0.3213232576172267 1105 76-77 0.1394657800287007 1105 78-79 0.0743505210532156 1105 80-81 0.12956883177352196 1105 82-83 0.13149807547040737 1105 84-85 0.4502704213711475 1105 86-87 0.39649689204387784 1105 88-89 0.20257177145987626 1105 90-91 0.30131468851670107 1105 92-93 0.26548678841067996 1105 94-95 0.5351160369983852 1105 96-97 0.8003781848480962 1105 98-99 0.3170875312710848 1105 100-101 0.6633666244718697 1106 1 0.28205163120041377 1106 2 0.2628721438761943 1106 3 0.1585064463930408 1106 4 0.20728516631680804 1106 5 0.2630340805352134 1106 6 0.14639483584740276 1106 7 0.15137904654508816 1106 8 0.2248046566934292 1106 9 0.24224351471912087 1106 10-11 0.251554343009623 1106 12-13 0.2534273346000191 1106 14-15 0.44110563954168924 1106 16-17 0.3269872585698721 1106 18-19 0.3770719495354484 1106 20-21 0.37929549316336875 1106 22-23 0.264872489361089 1106 24-25 0.3252646679677511 1106 26-27 0.28940116949357275 1106 28-29 0.07640855126677337 1106 30-31 0.21228814825578013 1106 32-33 0.36378728525887993 1106 34-35 0.31319120599253125 1106 36-37 0.11169795137912786 1106 38-39 0.3829093470773586 1106 40-41 0.4590316290854801 1106 42-43 0.36098286927395407 1106 44-45 0.33890403654297074 1106 46-47 0.3726569987059065 1106 48-49 -0.08316739362781078 1106 50-51 0.24326455955282 1106 52-53 0.19095884727058632 1106 54-55 0.41509516985428263 1106 56-57 0.17553866648739103 1106 58-59 0.14243343507747142 1106 60-61 0.12813748278438908 1106 62-63 0.18766118398038856 1106 64-65 0.2132783816410857 1106 66-67 0.19356801156110492 1106 68-69 0.03081724624625437 1106 70-71 0.29680949847011817 1106 72-73 0.3865585281764119 1106 74-75 0.25884779512141876 1106 76-77 0.2636267419737095 1106 78-79 0.05472051769876529 1106 80-81 0.12514850883445305 1106 82-83 0.30391312382417723 1106 84-85 0.43465300163132525 1106 86-87 0.7357980979452634 1106 88-89 0.5384999848437957 1106 90-91 0.605192753876107 1106 92-93 0.627369812642339 1106 94-95 0.7365472581614974 1106 96-97 0.9235867344119875 1106 98-99 0.9706926477276951 1106 100-101 1.417632152757104 1107 1 0.10893410393314085 1107 2 0.22299093825723304 1107 3 0.13460192114643377 1107 4 0.11046011810138623 1107 5 0.10820586420660305 1107 6 0.03237136498147919 1107 7 0.08112163241720083 1107 8 0.08858894093197023 1107 9 0.04203263595238127 1107 10-11 0.05871994695338856 1107 12-13 -0.029236188502288485 1107 14-15 0.24820943305803667 1107 16-17 0.3263366051876275 1107 18-19 0.15823640538341266 1107 20-21 0.11759343125956434 1107 22-23 0.11585461759931093 1107 24-25 0.17958916219078702 1107 26-27 0.23114319775671532 1107 28-29 0.20269596712403626 1107 30-31 0.1298471755700703 1107 32-33 0.10402304601043966 1107 34-35 0.12504421027519896 1107 36-37 0.31863844043112266 1107 38-39 0.06990117823757203 1107 40-41 0.06761545355964671 1107 42-43 0.13522784503965823 1107 44-45 0.04679847748410282 1107 46-47 0.010332163867964539 1107 48-49 0.14006129377515464 1107 50-51 0.04262035023641175 1107 52-53 0.3090157773044737 1107 54-55 0.0024454686156403227 1107 56-57 -0.028342206407351966 1107 58-59 0.038616246649702646 1107 60-61 0.24868968004645353 1107 62-63 0.19641804477343783 1107 64-65 0.004329546866273404 1107 66-67 0.1491736584039387 1107 68-69 0.26803662526187466 1107 70-71 0.20785590955286182 1107 72-73 0.3644660676676885 1107 74-75 0.07288717888460994 1107 76-77 -0.0472465012785932 1107 78-79 0.11296714656462825 1107 80-81 0.2537878443329049 1107 82-83 0.24388500665527602 1107 84-85 0.3905861122279326 1107 86-87 0.40617854112602814 1107 88-89 0.476851316628359 1107 90-91 0.47407722032389543 1107 92-93 0.8713413232957592 1107 94-95 0.7843676515256881 1107 96-97 1.0331439087285723 1107 98-99 1.0499038611913214 1107 100-101 1.303554762156633 1108 1 0.07546753326355926 1108 2 0.13310800330745565 1108 3 0.06219380982814471 1108 4 0.15559726021932363 1108 5 0.13681094900330493 1108 6 0.1007529560155973 1108 7 0.07229765335537053 1108 8 0.10575604250376358 1108 9 0.12031817327691385 1108 10-11 0.127168178433557 1108 12-13 0.04397781487677577 1108 14-15 0.13775942711266964 1108 16-17 0.17142370163725928 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-1.8411335345858113 2316 92-93 -1.70877035495975 2316 94-95 -2.0652616790992404 2316 96-97 -2.374062876170342 2316 98-99 -3.150937595906811 2316 100-101 -3.7817230662590404 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 12.0 4 44.0 5 79.0 6 191.0 7 353.0 8 915.0 9 1688.0 10 2770.0 11 4322.0 12 6201.0 13 8326.0 14 10657.0 15 14490.0 16 17995.0 17 23106.0 18 19577.0 19 12649.0 20 11871.0 21 11943.0 22 12827.0 23 14239.0 24 16707.0 25 19718.0 26 24441.0 27 29785.0 28 36807.0 29 45034.0 30 54702.0 31 66558.0 32 82423.0 33 104148.0 34 135679.0 35 180587.0 36 242597.0 37 288772.0 38 154202.0 39 6993.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.55584231132095 19.53700702745379 15.253429093189677 21.65372156803558 2 16.21290447485158 21.307041941888137 38.61950954962885 23.86054403363143 3 19.357662530835363 25.598625357654115 30.138059447990674 24.905652663519852 4 13.055530506609916 17.37250587648265 36.63185865180563 32.94010496510181 5 13.683254305926546 38.04551906487342 32.94495977087163 15.326266858328419 6 31.31392741416728 36.49996092364481 17.003564965943454 15.182546696244462 7 28.474158505720176 33.99552726026656 19.863352991745874 17.66696124226739 8 27.608466944409376 33.17762908723646 19.297166663660793 19.91673730469337 9 27.570172116315277 16.226426437258404 19.97992076517515 36.22348068125116 10-11 25.5658857407374 26.558906102524332 26.125729675786484 21.74947848095178 12-13 27.048721557348816 24.083483678822358 27.22264338330555 21.64515138052328 14-15 23.58416298120754 25.173521926239918 26.484927166029472 24.757387926523073 16-17 22.115750811811523 28.716951538391477 26.834733118934334 22.332564530862665 18-19 21.994149882874414 26.029774817683577 30.5209165283694 21.45515877107261 20-21 23.14844205577699 24.92244846429924 30.56546491845065 21.36364456147312 22-23 22.4622514553701 25.055661312299414 30.585661910468776 21.896425321861706 24-25 22.407885007304273 25.420551758135396 30.70295357127828 21.468609663282052 26-27 22.20571631944152 25.650603771361652 30.59563129968053 21.5480486095163 28-29 21.72555519584069 25.79711660983873 30.83302446952829 21.64430372479228 30-31 21.934899558640566 25.485283459887633 30.705216205403023 21.874600776068778 32-33 21.658036944669572 25.70615014994793 30.550147783488175 22.085665121894323 34-35 22.534775619251906 25.58690808703734 30.198036766146963 21.68027952756379 36-37 22.707194659591973 25.292932790707674 30.08686759927822 21.913004950422135 38-39 22.31003183870952 25.45079789152715 30.256272754817886 21.98289751494544 40-41 22.280165600029576 25.519844680085523 30.291321027855204 21.908668692029696 42-43 22.331647119086725 25.779267861566098 29.645426058683622 22.243658960663552 44-45 22.397094576558942 25.75575397445671 29.470256803649452 22.3768946453349 46-47 22.694720376192322 26.182206432650627 28.751966223489234 22.371106967667817 48-49 23.07921449805969 26.250199140913615 28.21025673396878 22.460329627057916 50-51 22.888207289968975 26.49670045070094 28.03546812599908 22.57962413333101 52-53 23.275045298622224 26.604268591071573 27.760991603974016 22.35969450633219 54-55 22.67161279520689 26.421999430680206 27.908242722611103 22.998145051501798 56-57 22.947737860738517 26.780453077235745 27.457976473078244 22.813832588947495 58-59 23.275048694143052 26.173846058555643 27.61843395787318 22.932671289428125 60-61 22.96595174706067 26.352466840949308 28.21099724445019 22.470584167539833 62-63 23.0509989985813 26.356943385119898 28.055356718208714 22.53670089809009 64-65 22.719008962665633 26.45692547888222 27.999498516444483 22.824567042007665 66-67 22.736786605661614 26.763426667536393 27.63335286208423 22.86643386471776 68-69 22.91943837415872 26.402907324521983 27.95047448425951 22.72717981705979 70-71 22.86727008647307 26.438071717822687 27.873738733972665 22.820919461731577 72-73 22.91873320468195 26.54057027431601 27.754041396889523 22.786655124112514 74-75 22.76062637803619 27.06191788069119 27.253787110223353 22.923668631049267 76-77 22.799213639861485 26.790352058484036 27.60256348595614 22.807870815698344 78-79 22.896272622902377 26.619170458757573 27.630221007978196 22.854335910361854 80-81 22.744098005991287 27.271806200776634 27.263448224441127 22.720647568790955 82-83 22.971438054653788 27.048825467338748 27.074530676988033 22.90520580101943 84-85 22.74114086664732 26.83025613347746 27.079549161781884 23.349053838093333 86-87 22.932182550061174 26.63994131847055 27.08308907747946 23.34478705398881 88-89 22.792605209408404 26.811679188589054 26.92420808764624 23.471507514356304 90-91 22.731768666342077 26.76695668230371 27.215467955081508 23.2858066962727 92-93 22.883537111916656 26.568419571403485 27.28890094268141 23.259142373998447 94-95 22.566087229490076 26.36080337823941 27.394072204936258 23.67903718733425 96-97 22.56029728122772 26.43832440068125 27.34182499179716 23.659553326293874 98-99 22.41376302795277 26.467789445065005 26.987734611421594 24.13071291556064 100-101 22.3274254755426 26.499489740828043 27.16720289246119 24.005881891168173 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 926.0 1 736.5 2 769.5 3 884.5 4 676.0 5 632.0 6 948.0 7 1888.5 8 2990.5 9 3478.0 10 3045.5 11 2346.5 12 2226.0 13 2414.0 14 2603.0 15 2750.0 16 2927.0 17 3105.5 18 3269.0 19 3463.5 20 3680.5 21 4052.5 22 4605.5 23 5266.5 24 5996.5 25 7018.0 26 8650.0 27 10514.5 28 12316.0 29 14712.0 30 17724.5 31 20682.0 32 24810.5 33 28219.5 34 30241.0 35 32229.5 36 35341.0 37 38993.0 38 41034.5 39 41648.0 40 42416.0 41 46144.5 42 49873.0 43 52536.0 44 54470.0 45 56278.5 46 57285.0 47 59684.0 48 69626.0 49 70768.5 50 62720.0 51 57978.0 52 54025.0 53 53793.5 54 54949.5 55 52776.5 56 48144.0 57 43389.0 58 40602.0 59 37017.0 60 32393.5 61 28020.5 62 23822.5 63 18876.5 64 14530.5 65 12120.0 66 9141.0 67 7109.0 68 5194.0 69 3327.5 70 2667.5 71 2360.0 72 2001.0 73 1854.5 74 1651.5 75 1411.0 76 1033.5 77 629.5 78 420.0 79 310.5 80 233.0 81 174.5 82 132.5 83 83.5 84 42.0 85 21.0 86 11.0 87 4.0 88 2.0 89 2.0 90 1.0 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021702406502305503 2 0.012263963785236353 3 0.008476563204501597 4 0.0 5 0.003787400580734756 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.001142231921173974 14-15 2.104111433741531E-4 16-17 3.005873476773616E-5 18-19 0.0014728780036190716 20-21 0.0 22-23 9.017620430320846E-5 24-25 0.0 26-27 0.0019237590251351142 28-29 0.003336519559218713 30-31 0.0015931129426900164 32-33 0.009979499942888404 34-35 0.0019237590251351142 36-37 9.919382473352932E-4 38-39 0.0015630542079222802 40-41 2.705286129096254E-4 42-43 0.007093861405185733 44-45 0.002284463842347948 46-47 3.306460824450977E-4 48-49 9.017620430320846E-4 50-51 6.011746953547232E-5 52-53 2.4046987814188927E-4 54-55 0.0012925255950126547 56-57 0.017674536043428862 58-59 0.028796267907491235 60-61 0.024197281488027605 62-63 0.016201658039809788 64-65 0.02071046825497021 66-67 0.0030359322115413516 68-69 0.0015029367383868077 70-71 1.2023493907094464E-4 72-73 0.0 74-75 0.002254405107580212 76-77 0.0030058734767736154 78-79 0.011392260476972003 80-81 0.01971853000763492 82-83 0.019417942659957558 84-85 0.030509615789252202 86-87 0.011722906559417101 88-89 0.0436452828827529 90-91 0.04800379942407464 92-93 0.029698029950523324 94-95 0.03198249379287127 96-97 0.052692962047841484 98-99 0.055728894259382836 100-101 0.06128976019141402 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1663410.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.17357999626609 #Duplication Level Percentage of deduplicated Percentage of total 1 72.22511346070958 32.626669406567586 2 14.664761279752334 13.24919533594075 3 5.31411099761664 7.201722547796181 4 2.5598842958005625 4.62556552070128 5 1.3712496292473175 3.097212741082696 6 0.8442269779937497 2.288205295524398 7 0.5517806465320103 1.7448135023553635 8 0.3610987780597047 1.3049699629787204 9 0.27151478454514294 1.1038765355837068 >10 1.5479233125101777 13.147066226520646 >50 0.16543414988376898 5.167119728168567 >100 0.1133963902135002 9.390045762774493 >500 0.006827748646649479 2.0821099170420547 >1k 0.0022759162155498265 1.4639721959404268 >5k 2.6775484888821486E-4 0.8714163411949275 >10k+ 1.3387742444410743E-4 0.6360389798283749 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10517 0.6322554271045623 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8856 0.5324003102061428 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5553 0.3338323083304777 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3010 0.18095358330177166 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2231 0.13412207453363875 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1803 0.10839179757245658 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 1.2023493907094462E-4 0.0 24-25 0.0 0.0 0.0 4.5088102151604235E-4 0.0 26-27 0.0 0.0 0.0 8.717033082643485E-4 0.0 28-29 0.0 0.0 0.0 0.0015931129426900162 0.0 30-31 0.0 0.0 0.0 0.0030960496810768243 0.0 32-33 0.0 0.0 0.0 0.00507992617574741 0.0 34-35 0.0 0.0 0.0 0.007364390018095359 0.0 36-37 0.0 0.0 0.0 0.011151790598830114 0.0 38-39 0.0 0.0 0.0 0.01746412490005471 0.0 40-41 0.0 0.0 0.0 0.025129102265827426 0.0 42-43 0.0 0.0 0.0 0.03168190644519391 0.0 44-45 0.0 0.0 0.0 0.03859541544177322 0.0 46-47 0.0 0.0 0.0 0.04719221358534577 0.0 48-49 0.0 0.0 0.0 0.055638718055079625 0.0 50-51 0.0 0.0 0.0 0.06381469391190386 0.0 52-53 0.0 0.0 0.0 0.0790544724391461 0.0 54-55 0.0 0.0 0.0 0.10307140151856728 0.0 56-57 0.0 0.0 0.0 0.1391719419746184 0.0 58-59 0.0 0.0 0.0 0.18534215857786113 0.0 60-61 0.0 0.0 0.0 0.23671253629592223 0.0 62-63 0.0 0.0 0.0 0.27636000745456624 0.0 64-65 0.0 0.0 0.0 0.3227706939359508 0.0 66-67 0.0 0.0 0.0 0.3976470022423816 0.0 68-69 0.0 0.0 0.0 0.5055578600585544 0.0 70-71 0.0 0.0 0.0 0.6127473082403015 0.0 72-73 0.0 0.0 0.0 0.7297058452215629 0.0 74-75 0.0 0.0 0.0 0.8444700945647796 0.0 76-77 0.0 0.0 0.0 0.9528017746677007 0.0 78-79 0.0 0.0 0.0 1.059269813215022 0.0 80-81 0.0 0.0 0.0 1.1699460746298267 0.0 82-83 0.0 0.0 0.0 1.3155205271099728 0.0 84-85 0.0 0.0 0.0 1.5262923753013389 0.0 86-87 0.0 0.0 0.0 1.7924324129348748 0.0 88-89 0.0 0.0 0.0 2.092598938325488 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10825 0.0 28.352415 1 GTATCAA 15335 0.0 27.759373 1 CGGATTA 70 0.008401211 27.137169 9 GTACATG 10910 0.0 24.168722 1 GAGTACT 10025 0.0 23.378597 12-13 TACATGG 11260 0.0 23.029419 2 ATCAACG 18860 0.0 22.511189 3 ACATGGG 10960 0.0 22.488445 3 TCAACGC 18940 0.0 22.440506 4 CAACGCA 19100 0.0 22.302248 5 GTACTTT 10515 0.0 22.15299 14-15 TATCAAC 19245 0.0 22.062176 2 AACGCAG 19655 0.0 21.672499 6 AGTACTT 10265 0.0 21.605963 12-13 TATATCG 110 0.0028819048 21.585737 4 GTAAGGT 600 0.0 21.36988 4 ACTTTTT 11825 0.0 20.000038 16-17 GATGTGC 930 0.0 19.915182 8 ACGCAGA 21555 0.0 19.762735 7 CGCAGAG 21710 0.0 19.621637 8 >>END_MODULE