##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936515_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1380794 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.413033370654855 31.0 31.0 34.0 30.0 34.0 2 31.461406987573817 31.0 31.0 34.0 28.0 34.0 3 31.471948024107867 31.0 31.0 34.0 28.0 34.0 4 35.09367363994919 37.0 35.0 37.0 32.0 37.0 5 35.12263596162788 37.0 35.0 37.0 32.0 37.0 6 35.13072985543101 37.0 35.0 37.0 32.0 37.0 7 35.050372466856025 37.0 35.0 37.0 32.0 37.0 8 35.01349585818015 37.0 35.0 37.0 32.0 37.0 9 36.5021176221797 39.0 37.0 39.0 32.0 39.0 10-11 36.353499870364445 39.0 37.0 39.0 32.0 39.0 12-13 36.267500076043206 39.0 35.0 39.0 32.0 39.0 14-15 37.29859052110597 39.5 37.0 41.0 32.0 41.0 16-17 37.208153424768646 39.0 36.5 41.0 31.0 41.0 18-19 37.2272442522201 39.0 36.5 41.0 31.0 41.0 20-21 37.089833458140745 39.0 36.0 41.0 31.0 41.0 22-23 37.203554259360914 39.0 36.5 41.0 31.0 41.0 24-25 37.23973887487924 39.0 36.5 41.0 31.0 41.0 26-27 37.1196445668217 39.0 36.0 41.0 31.0 41.0 28-29 36.98438543330866 39.0 36.0 41.0 30.5 41.0 30-31 36.77099444232811 39.0 36.0 41.0 30.0 41.0 32-33 36.564623687530506 39.0 35.0 41.0 30.0 41.0 34-35 36.352516016147234 39.0 35.0 40.0 30.0 41.0 36-37 36.11463911343763 38.5 35.0 40.0 30.0 41.0 38-39 35.88514579292784 38.0 35.0 40.0 29.0 41.0 40-41 35.68197573280301 38.0 35.0 40.0 27.5 41.0 42-43 35.4276452533832 38.0 35.0 40.0 27.0 41.0 44-45 35.11861725934499 38.0 34.0 40.0 26.5 41.0 46-47 34.851978644171396 38.0 34.0 40.0 24.5 41.0 48-49 34.625784512389245 38.0 34.0 40.0 24.0 41.0 50-51 33.63088049339727 37.5 32.5 39.5 22.5 40.5 52-53 33.719138046660106 37.5 33.0 39.5 23.0 40.5 54-55 34.16421819619726 38.0 33.5 40.0 23.0 41.0 56-57 34.032390783853344 38.0 33.0 40.0 22.5 41.0 58-59 33.79345579427489 38.0 33.0 40.0 22.0 41.0 60-61 33.44193123666528 37.0 33.0 40.0 20.5 41.0 62-63 33.07087443890978 36.5 32.0 40.0 19.5 41.0 64-65 32.69546181399977 36.0 32.0 39.0 18.0 41.0 66-67 32.20470794340068 35.5 31.0 39.0 14.5 40.5 68-69 31.806669206268275 35.0 31.0 38.5 13.0 40.0 70-71 31.374478379830734 35.0 31.0 38.0 9.5 40.0 72-73 30.948194661911913 35.0 30.0 37.0 7.5 39.5 74-75 30.522577589415945 35.0 30.0 37.0 6.5 39.0 76-77 30.050735301572864 34.0 29.5 36.0 2.0 39.0 78-79 29.54974818836119 34.0 29.0 36.0 2.0 37.5 80-81 29.070534779264683 34.0 29.0 35.0 2.0 37.0 82-83 28.651547225726645 34.0 28.5 35.0 2.0 37.0 84-85 28.26729656994454 34.0 27.0 35.0 2.0 36.0 86-87 27.915257453320336 34.0 27.0 35.0 2.0 36.0 88-89 27.45979052632036 33.0 26.0 35.0 2.0 35.5 90-91 27.00532881805686 33.0 25.0 35.0 2.0 35.0 92-93 26.555245749909112 33.0 25.0 35.0 2.0 35.0 94-95 26.042860846730214 33.0 24.0 35.0 2.0 35.0 96-97 25.40302608499168 32.0 20.0 35.0 2.0 35.0 98-99 24.61103792455645 32.0 10.0 35.0 2.0 35.0 100-101 21.674069774347224 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.44223162569567265 1101 2 0.5542552292049088 1101 3 0.4365805684572095 1101 4 0.5302085246961354 1101 5 0.4512358952262119 1101 6 0.5533616050797008 1101 7 0.5777764426064138 1101 8 0.6898849001003171 1101 9 0.6287528570812881 1101 10-11 0.7447187336486394 1101 12-13 0.811223142547675 1101 14-15 0.8970783363165467 1101 16-17 0.9016762025515206 1101 18-19 0.8423149993036958 1101 20-21 0.9388068006566286 1101 22-23 0.8540773918564284 1101 24-25 0.9124571809325488 1101 26-27 0.73294742574992 1101 28-29 0.8020558798055433 1101 30-31 0.8402585621205603 1101 32-33 0.6927936463028885 1101 34-35 0.6390253273054967 1101 36-37 0.533776216669466 1101 38-39 0.6661182804558266 1101 40-41 0.2922771444743546 1101 42-43 0.4556187293633087 1101 44-45 0.5000810648135214 1101 46-47 0.6127237108986421 1101 48-49 0.7734004762492575 1101 50-51 0.3069816669109926 1101 52-53 0.5531375103987983 1101 54-55 0.6857685292424307 1101 56-57 0.5857487343715775 1101 58-59 0.5937679175543025 1101 60-61 0.5364456802908677 1101 62-63 0.7046269042440585 1101 64-65 0.7072408348322199 1101 66-67 0.6225561195261164 1101 68-69 0.41313220847938936 1101 70-71 0.42778799694879055 1101 72-73 0.6203458539673683 1101 74-75 0.5274667796196724 1101 76-77 0.40350918581437867 1101 78-79 0.5165583053268783 1101 80-81 0.5100509187001592 1101 82-83 0.5127883302301406 1101 84-85 0.459516291005059 1101 86-87 0.529014599989349 1101 88-89 0.5545950492117164 1101 90-91 0.4331065020869964 1101 92-93 0.2653466842295309 1101 94-95 0.19892069491949727 1101 96-97 0.35015014322190297 1101 98-99 0.4766991615959739 1101 100-101 0.4946775736264293 1102 1 0.36903093790397534 1102 2 0.485126992252912 1102 3 0.53172077712183 1102 4 0.2973031219113551 1102 5 0.3779705653576926 1102 6 0.3408572417395206 1102 7 0.43922261514904193 1102 8 0.5470776239881516 1102 9 0.5370257500727931 1102 10-11 0.5765135855536414 1102 12-13 0.5397910619777875 1102 14-15 0.5640940701980455 1102 16-17 0.4522780202867267 1102 18-19 0.6371798327210598 1102 20-21 0.6093132066864442 1102 22-23 0.39534489369872716 1102 24-25 0.6193635404200677 1102 26-27 0.36600667667753584 1102 28-29 0.19237120385626838 1102 30-31 0.49450102162229825 1102 32-33 0.4490413030276059 1102 34-35 0.3664102967298035 1102 36-37 0.3026355425818963 1102 38-39 0.4782360841766291 1102 40-41 0.26556057412438605 1102 42-43 0.3187973095647294 1102 44-45 0.37616220354835406 1102 46-47 0.2745422218037561 1102 48-49 0.5005268773662763 1102 50-51 0.5122008681934815 1102 52-53 0.7958361878219051 1102 54-55 0.706434601201984 1102 56-57 0.4277958196603322 1102 58-59 0.6782938972049806 1102 60-61 0.6868613288448273 1102 62-63 0.7444722803963089 1102 64-65 0.7353342814184742 1102 66-67 0.7774514640037395 1102 68-69 0.5452218282546966 1102 70-71 0.3714653554149656 1102 72-73 0.2114136117922918 1102 74-75 0.6500346214792927 1102 76-77 0.642193590266924 1102 78-79 0.6773942566643214 1102 80-81 0.7828792681293706 1102 82-83 0.5864996904287239 1102 84-85 0.551527823151563 1102 86-87 0.58072179723818 1102 88-89 0.7204342972950748 1102 90-91 0.70159736816154 1102 92-93 0.6287644401151873 1102 94-95 0.4659700426809401 1102 96-97 0.9183216774369534 1102 98-99 1.1091960264552512 1102 100-101 1.0022536250298124 1103 1 0.45416871372770373 1103 2 0.34420585890185507 1103 3 0.3597710479303373 1103 4 0.5415392026032535 1103 5 0.4551164716633096 1103 6 0.4342608630960001 1103 7 0.5876656354476353 1103 8 0.38455516039149273 1103 9 0.37309550458270024 1103 10-11 0.6509782088068476 1103 12-13 0.21047135091961167 1103 14-15 0.6232869671803556 1103 16-17 0.7428149645445217 1103 18-19 0.9395513496114631 1103 20-21 0.9000702522287298 1103 22-23 0.6166241126180196 1103 24-25 0.5326948017872297 1103 26-27 0.5287828117422961 1103 28-29 0.6194359190484562 1103 30-31 0.5069414819236613 1103 32-33 0.6021378546658553 1103 34-35 0.8892261095857705 1103 36-37 0.929906064657807 1103 38-39 0.9855476160220036 1103 40-41 1.1627892471762422 1103 42-43 0.8503359556142485 1103 44-45 1.0403504521431373 1103 46-47 1.0918532825836564 1103 48-49 1.0915413304132286 1103 50-51 1.1997574840374199 1103 52-53 1.2673998057563978 1103 54-55 1.1758045159283057 1103 56-57 1.1063680797512916 1103 58-59 1.0713752989442398 1103 60-61 1.1669236177790623 1103 62-63 1.2896907762679888 1103 64-65 1.2001191588087963 1103 66-67 1.308986319871238 1103 68-69 1.0095491765296458 1103 70-71 1.1431428953919074 1103 72-73 1.1181567246254716 1103 74-75 1.036926385251327 1103 76-77 1.3173950249049646 1103 78-79 1.2004659068066985 1103 80-81 1.140204634139124 1103 82-83 0.9185212598131507 1103 84-85 1.135713180378925 1103 86-87 1.1812737018858677 1103 88-89 0.9626261162413314 1103 90-91 1.0326034394916839 1103 92-93 0.9954667164443656 1103 94-95 0.9081066874060646 1103 96-97 1.0337178299690386 1103 98-99 1.0084519054336525 1103 100-101 1.2554039431073 1104 1 0.5082495493020183 1104 2 0.44847607543813695 1104 3 0.3364423494145008 1104 4 0.4187156605414941 1104 5 0.46294839961737466 1104 6 0.517780871698001 1104 7 0.6722113477846463 1104 8 0.6143043637053722 1104 9 0.48895627874129133 1104 10-11 0.7653254638107114 1104 12-13 0.6516001204862434 1104 14-15 0.41071830142799826 1104 16-17 0.5090514962533135 1104 18-19 0.3788445522422421 1104 20-21 0.1855600758350917 1104 22-23 0.4547885140571637 1104 24-25 0.6477212033802857 1104 26-27 0.28950341868902996 1104 28-29 0.27266135144108006 1104 30-31 0.3116574526473528 1104 32-33 0.14469823467169363 1104 34-35 0.2525178806588215 1104 36-37 0.26173914376138896 1104 38-39 0.1404826424868446 1104 40-41 0.16885043941716305 1104 42-43 0.37980728071173075 1104 44-45 0.28945625564444555 1104 46-47 0.012765515866426824 1104 48-49 0.23415578914630686 1104 50-51 0.11994629574963511 1104 52-53 0.23208602790927557 1104 54-55 -0.13152077973896326 1104 56-57 0.09697862120268042 1104 58-59 0.17316479881615976 1104 60-61 0.03738082021166633 1104 62-63 0.24161499858320923 1104 64-65 0.16120803872555456 1104 66-67 0.32569406388173405 1104 68-69 0.19275272443149305 1104 70-71 0.060824372742619204 1104 72-73 0.001202516552034183 1104 74-75 0.14953400244561976 1104 76-77 -0.06779230570369776 1104 78-79 0.1080468904913694 1104 80-81 0.08772744893160933 1104 82-83 -0.16554296210148678 1104 84-85 -0.18388788095562703 1104 86-87 -0.22406796919951333 1104 88-89 -0.2233330221675338 1104 90-91 -0.2874677799783427 1104 92-93 -0.39131688536753373 1104 94-95 -0.2767352983479334 1104 96-97 -0.13467257416666456 1104 98-99 -0.053854852720583324 1104 100-101 0.026355233248633425 1105 1 0.4164778129943123 1105 2 0.4190238265413342 1105 3 0.4508082139346463 1105 4 0.3838326055452015 1105 5 0.40129739506635786 1105 6 0.5507236477878408 1105 7 0.5821977115242376 1105 8 0.627852743794655 1105 9 0.7155408645337147 1105 10-11 0.6699839599118746 1105 12-13 0.44454051689560004 1105 14-15 0.49800789471986917 1105 16-17 0.5552365524522784 1105 18-19 0.43804922550026504 1105 20-21 0.570282329599884 1105 22-23 0.3198902528037948 1105 24-25 0.2921455922418019 1105 26-27 0.21213171160894007 1105 28-29 0.16390723760546422 1105 30-31 0.06666564329037783 1105 32-33 -0.001236905704764979 1105 34-35 0.16183748952229138 1105 36-37 0.4235332253676489 1105 38-39 0.17976662526230314 1105 40-41 0.20126420562194625 1105 42-43 0.24803222667521396 1105 44-45 0.19897359522089886 1105 46-47 0.19653017040297982 1105 48-49 0.30603003932478856 1105 50-51 0.35999804639465793 1105 52-53 0.6706840998743644 1105 54-55 0.39948270486371484 1105 56-57 0.302113991450355 1105 58-59 0.21037297938750044 1105 60-61 0.2441597498688992 1105 62-63 0.3205334979823604 1105 64-65 0.3304707129927209 1105 66-67 0.36558259411799554 1105 68-69 0.5512703513469575 1105 70-71 0.478537045578026 1105 72-73 0.411246397465316 1105 74-75 0.6517087934336416 1105 76-77 0.4682635565552573 1105 78-79 0.48648742958280167 1105 80-81 0.6952507446212266 1105 82-83 0.4424202288821881 1105 84-85 0.4110119996653836 1105 86-87 0.2895500239497686 1105 88-89 0.14125575225751774 1105 90-91 0.1747441774717835 1105 92-93 0.19102400929358865 1105 94-95 0.4669689162538262 1105 96-97 0.367254868289006 1105 98-99 0.4738168323400558 1105 100-101 0.46639814649101297 1106 1 0.3176898304320339 1106 2 0.1817837334427601 1106 3 0.28736918263791367 1106 4 0.32798864004308825 1106 5 0.2435088798481928 1106 6 0.2856365137278871 1106 7 0.43007536478375386 1106 8 0.517761831814731 1106 9 0.39122078340685107 1106 10-11 0.41465230217578153 1106 12-13 0.5626739161271175 1106 14-15 0.5563035951006938 1106 16-17 0.4304919118574517 1106 18-19 0.23531864075983577 1106 20-21 0.08152407236645587 1106 22-23 0.28372111582491044 1106 24-25 0.2315485148767351 1106 26-27 0.17158938918185385 1106 28-29 0.2047932013402729 1106 30-31 0.27698859022921596 1106 32-33 0.3665558166885532 1106 34-35 0.3844871750750727 1106 36-37 0.5025368126129308 1106 38-39 0.2777525409489954 1106 40-41 0.163226504209959 1106 42-43 0.4023371344612343 1106 44-45 0.09467691680229251 1106 46-47 0.1849269995115037 1106 48-49 0.1988113425814788 1106 50-51 -0.0831009768482005 1106 52-53 -0.17125204497767754 1106 54-55 -0.01248843970137159 1106 56-57 -0.09745167819010447 1106 58-59 0.018141597086184902 1106 60-61 0.2027622372722746 1106 62-63 0.319073081713924 1106 64-65 0.32058385137937506 1106 66-67 0.3450566071037926 1106 68-69 0.3364442566157919 1106 70-71 0.3509268301583255 1106 72-73 0.12755306685771473 1106 74-75 0.14031906434821195 1106 76-77 0.06073603323437027 1106 78-79 -0.0013361896977244214 1106 80-81 0.24464070422380857 1106 82-83 0.2751363991859783 1106 84-85 0.4953888657989509 1106 86-87 0.44564381122797414 1106 88-89 0.36887815757591724 1106 90-91 0.2384565565747856 1106 92-93 0.08598170749920442 1106 94-95 -0.008476975536868991 1106 96-97 0.0010390065272041227 1106 98-99 0.03431691226215605 1106 100-101 -0.08948779589962541 1107 1 0.14978610844075746 1107 2 0.28214129508049623 1107 3 0.31797166209899785 1107 4 0.2452781893791709 1107 5 0.3494447389923323 1107 6 0.360945163191964 1107 7 0.28074497045808045 1107 8 0.37457106430538545 1107 9 0.6620955379127693 1107 10-11 0.4522011735212459 1107 12-13 0.4026050074716707 1107 14-15 0.20278464825340592 1107 16-17 0.3294773662892254 1107 18-19 0.10896658602858622 1107 20-21 0.5332198888066344 1107 22-23 0.28757949659281934 1107 24-25 0.35688243115887985 1107 26-27 0.25710322014007403 1107 28-29 0.4649184762306575 1107 30-31 0.427096819932828 1107 32-33 0.1917171347065718 1107 34-35 0.18737823099476714 1107 36-37 0.28330948730743444 1107 38-39 0.23655012970598932 1107 40-41 0.16559220628920457 1107 42-43 0.23567132324239992 1107 44-45 0.3560580668694584 1107 46-47 0.26019598730364635 1107 48-49 0.39329608380306524 1107 50-51 0.08731029602549967 1107 52-53 0.3059529923346247 1107 54-55 0.5841418579898061 1107 56-57 0.264360057651956 1107 58-59 0.46909527365318127 1107 60-61 0.5828960324230081 1107 62-63 0.7009862861110534 1107 64-65 0.4531179942463126 1107 66-67 0.479257940015934 1107 68-69 0.6179947666882271 1107 70-71 0.4118133291852146 1107 72-73 0.43911929695046936 1107 74-75 0.3953228508713593 1107 76-77 0.4302603311411204 1107 78-79 0.6107154992595767 1107 80-81 0.4095513698073212 1107 82-83 0.508937522497849 1107 84-85 0.07677368340425161 1107 86-87 0.1999054578779429 1107 88-89 0.07161369308081689 1107 90-91 0.11153500689527718 1107 92-93 0.48012120553175563 1107 94-95 0.4625436406002059 1107 96-97 0.434993923804619 1107 98-99 0.35842929570933535 1107 100-101 0.2626649230013136 1108 1 0.2302590939465894 1108 2 0.20156521048032516 1108 3 0.20495526335956882 1108 4 0.32419042029168565 1108 5 0.2419229696645928 1108 6 0.26165014927588714 1108 7 0.18965231797152882 1108 8 0.15108674756471885 1108 9 0.26502824688289905 1108 10-11 0.3320343462992241 1108 12-13 0.12998982990419705 1108 14-15 0.2816356640786566 1108 16-17 0.34758207354532544 1108 18-19 0.518238692614645 1108 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-3.413192615863167 2316 94-95 -3.3888901023016693 2316 96-97 -3.640446840561399 2316 98-99 -4.801651838735815 2316 100-101 -5.593731815023592 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2607.0 3 1137.0 4 1131.0 5 1227.0 6 1445.0 7 1783.0 8 2322.0 9 2878.0 10 3680.0 11 4886.0 12 6427.0 13 7865.0 14 9032.0 15 10559.0 16 13050.0 17 16565.0 18 13255.0 19 10042.0 20 10277.0 21 11073.0 22 12170.0 23 14029.0 24 15485.0 25 17797.0 26 20558.0 27 24332.0 28 28858.0 29 34135.0 30 41094.0 31 50986.0 32 62463.0 33 78424.0 34 103295.0 35 138447.0 36 186291.0 37 230152.0 38 168435.0 39 22595.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.00736331049109 18.359240606998696 14.08575366735017 21.547642415160045 2 15.224467387005793 21.25461559299053 39.60001332709471 23.920903692908972 3 20.900113792671345 25.923143951713083 29.3411976551901 23.83554460042547 4 12.32696912933691 16.47681259570572 38.364502431667745 32.83171584328963 5 13.111969167910287 38.754473405873746 33.51142439038526 14.622133035830714 6 30.0092819831707 36.41203889882783 18.033277467532987 15.545401650468483 7 27.76072531009498 33.831677417205405 20.684128264815246 17.723469007884372 8 26.024241616416845 32.706043597819814 20.106908252175398 21.16280653358794 9 26.692487589438635 17.766736507770908 20.91002089437185 34.6307550084186 10-11 24.962288893880004 26.3157951935547 26.919969860095783 21.801946052469514 12-13 27.77581776572632 23.98388266393891 26.83636426860198 21.403935301732787 14-15 23.97850502258197 25.197522050426123 27.157547854251128 23.66642507274078 16-17 21.510215672652222 28.5510718433126 27.645595883449403 22.29311660058578 18-19 21.24829269052415 28.156272377027534 29.617703934517554 20.977730997930767 20-21 23.0309227028443 26.21609116775259 28.833290881811703 21.919695247591406 22-23 22.08709735364504 26.254440047051464 28.985467356600243 22.672995242703255 24-25 22.636548383639106 26.31829954025186 29.28189339753005 21.763258678578982 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27.470888756592487 28.479552239454552 21.854906578945464 52-53 22.428870595162522 27.93439462375443 27.527076956169534 22.109657824913516 54-55 21.710323809082357 27.25170400102555 28.03690970152551 23.001062488366586 56-57 22.61455999965237 27.44471796075125 27.208004443855405 22.73271759574097 58-59 23.297425102059073 26.896191086303965 27.26604091923237 22.54034289240459 60-61 22.543822102867058 27.04133179504227 28.46330951299762 21.951536589093052 62-63 22.666107083018144 27.075037197948852 28.18309972888679 22.07575599014621 64-65 22.23583767054164 27.970150302237606 27.81216040591417 21.981851621306582 66-67 22.145131089533482 27.36125950258775 27.42511109742891 23.068498310449858 68-69 22.006548463922222 27.65820322264415 27.701168913127432 22.634079400306195 70-71 22.735719547008042 27.528460545446837 26.983615145962986 22.75220476158213 72-73 22.39105760016341 27.091148649578546 27.70951687311536 22.808276877142685 74-75 21.90308529616851 28.59268113776522 27.091997428165215 22.412236137901058 76-77 22.494078732662253 27.271515507580922 27.29614595131648 22.938259808440346 78-79 22.242580456288533 27.35495694215379 27.81524546305663 22.587217138501046 80-81 22.645758518352615 27.817818024598207 27.07031149487897 22.466111962170213 82-83 22.34298239258025 27.586696616187233 27.530686182160714 22.539634809071806 84-85 22.249482531007395 27.697475765428543 27.298132765430356 22.75490893813371 86-87 22.332794877181293 28.013603865294208 27.147317256916022 22.506284000608478 88-89 22.45551241805777 27.570547556465392 26.706203940036342 23.267736085440497 90-91 22.20257946441445 27.179724230170937 27.27434758069249 23.343348724722127 92-93 22.556634444869434 27.843421479652747 27.340861411500168 22.259082663977654 94-95 22.14914218580011 27.662425521620275 27.294506309566408 22.893925983013208 96-97 21.40017884610579 27.53693997862394 27.699016998348082 23.36386417692219 98-99 21.917638981727542 27.894709193109833 26.805897473520606 23.38175435164202 100-101 22.14298552287525 27.432723693898968 27.09985708405081 23.32443369917497 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 653.0 1 459.0 2 523.5 3 768.5 4 661.5 5 624.5 6 875.5 7 1442.0 8 2090.5 9 2341.5 10 2072.0 11 1780.5 12 1741.5 13 1821.0 14 1902.5 15 1940.0 16 1978.0 17 2096.5 18 2238.5 19 2344.5 20 2615.0 21 3011.5 22 3456.5 23 4079.5 24 4786.5 25 5712.5 26 7119.0 27 8853.0 28 10604.0 29 12623.5 30 15206.5 31 18101.5 32 21614.0 33 24204.0 34 26280.0 35 28560.5 36 31316.0 37 34241.5 38 35417.0 39 36156.5 40 36939.0 41 37993.5 42 39042.5 43 40734.0 44 42922.0 45 45024.0 46 47906.0 47 57538.0 48 84058.0 49 83404.0 50 57796.5 51 48250.5 52 43827.0 53 42306.0 54 41313.5 55 38460.5 56 34811.0 57 31417.0 58 29408.5 59 26720.0 60 22610.0 61 19534.0 62 16505.5 63 12414.0 64 8554.5 65 6223.5 66 4698.0 67 3669.5 68 2903.5 69 2278.0 70 1736.5 71 1304.0 72 1077.5 73 978.0 74 755.5 75 520.0 76 400.0 77 255.0 78 162.5 79 105.0 80 69.5 81 60.0 82 47.5 83 24.5 84 8.0 85 5.0 86 7.5 87 7.5 88 5.0 89 3.0 90 2.0 91 2.5 92 3.5 93 2.5 94 1.0 95 1.0 96 0.5 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011949646362889758 2 0.010718470677016267 3 0.015425907123003142 4 0.018829745783947496 5 0.02998274905597794 6 0.050767891517489214 7 0.006373144726874537 8 0.02592711150251232 9 0.03751464736955693 10-11 0.04084606393133226 12-13 0.030200015353485023 14-15 0.02741176453547741 16-17 0.03005517115514697 18-19 0.024080347973702086 20-21 0.05710482519477924 22-23 0.012891133652087133 24-25 0.002752039768423096 26-27 0.0011587535867044613 28-29 3.6211049584514415E-5 30-31 0.0027158287188385815 32-33 0.002064029826317322 34-35 0.0 36-37 1.0863314875354324E-4 38-39 0.0 40-41 0.001303597785042519 42-43 0.0036211049584514415 44-45 0.0014484419833805768 46-47 6.517988925212595E-4 48-49 6.88009942105774E-4 50-51 0.004055637553465615 52-53 0.003874582305543043 54-55 0.0052868132393391045 56-57 7.966430908593171E-4 58-59 0.00713357676814934 60-61 0.0029693060659301824 62-63 3.9832154542965856E-4 64-65 6.88009942105774E-4 66-67 0.01803310269308818 68-69 0.04511896778230497 70-71 0.05572880531056768 72-73 0.016294972313031487 74-75 0.033060688270661664 76-77 0.0281359855271677 78-79 0.05721345834353278 80-81 0.0825974041022774 82-83 0.05026093682330601 84-85 0.020024710420236472 86-87 0.022016318147384768 88-89 0.02350097118034986 90-91 0.042366928013881866 92-93 0.026216799899188438 94-95 0.013905043040453535 96-97 0.0200971325194055 98-99 0.051528323558764015 100-101 0.06967005940060574 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1380794.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.23274625102216 #Duplication Level Percentage of deduplicated Percentage of total 1 78.19888667063456 43.973381512622325 2 12.849264841125665 14.450988986464 3 4.096735118937981 6.911119992026717 4 1.713130718910735 3.8533618004535413 5 0.8801959126671737 2.474791670410002 6 0.5235472776605516 1.7664300729059546 7 0.3377908902943464 1.3296436593880188 8 0.22459992579998606 1.0103896508407217 9 0.15871185085031692 0.8032322912306352 >10 0.9077394433227741 9.261065684463873 >50 0.06621950550344519 2.531308285161872 >100 0.03615497643959806 3.8478761002963693 >500 0.003121274601242156 1.2477855962404696 >1k 0.003381380818012336 4.420051204499926 >5k 3.901593251552695E-4 1.3777419322221431 >10k+ 1.3005310838508984E-4 0.7408315607734121 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10130 0.733635864582262 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6680 0.48377962244911266 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6514 0.47175755398705377 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5645 0.4088227498091678 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4945 0.35812728039084757 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4848 0.3511023367714518 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4707 0.34089082078861876 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4254 0.3080836098650487 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3256 0.23580635489435792 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3131 0.22675359249822927 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2986 0.21625238811872008 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2829 0.20488211854918256 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2541 0.18402455398850226 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2380 0.17236459602228862 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2119 0.1534624281391721 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1932 0.1399194955945637 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1901 0.1376744105203238 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1824 0.1320979088843086 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1782 0.12905618071920938 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1743 0.12623171885161724 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1619 0.11725137855465768 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1526 0.110516123331938 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1406 0.10182547143165455 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 1.4484419833805766E-4 14-15 0.0 0.0 0.0 0.0 1.8105524792257207E-4 16-17 0.0 0.0 0.0 3.6211049584514415E-5 2.1726629750708652E-4 18-19 0.0 0.0 0.0 7.242209916902883E-5 2.1726629750708652E-4 20-21 0.0 0.0 0.0 1.4484419833805766E-4 2.534773470916009E-4 22-23 0.0 0.0 0.0 2.172662975070865E-4 2.896883966761153E-4 24-25 0.0 0.0 0.0 5.793767933522306E-4 3.6211049584514415E-4 26-27 0.0 0.0 0.0 9.052762396128604E-4 4.3453259501417303E-4 28-29 0.0 0.0 0.0 0.0019553966775637784 4.3453259501417303E-4 30-31 0.0 0.0 0.0 0.004272903850972701 4.707436445986874E-4 32-33 0.0 0.0 0.0 0.00713357676814934 5.069546941832018E-4 34-35 0.0 0.0 0.0 0.010175304933248551 5.793767933522306E-4 36-37 0.0 0.0 0.0 0.011949646362889758 5.793767933522306E-4 38-39 0.0 0.0 0.0 0.013615354643777422 6.517988925212595E-4 40-41 0.0 0.0 0.0 0.016186339164277946 6.517988925212595E-4 42-43 0.0 0.0 0.0 0.01966259992439133 6.517988925212595E-4 44-45 0.0 0.0 0.0 0.023066438585335684 6.517988925212595E-4 46-47 0.0 0.0 0.0 0.026542699345449068 6.517988925212595E-4 48-49 0.0 0.0 0.0 0.030996658444344342 7.604320412748027E-4 50-51 0.0 0.0 0.0 0.03914414460086009 8.690651900283461E-4 52-53 0.0 0.0 0.0 0.05489595117012386 9.776983387818892E-4 54-55 0.0 0.0 0.0 0.07680363616875507 0.0011225425371199469 56-57 0.0 0.0 0.0 0.10378086810921833 0.00123117568587349 58-59 0.0 0.0 0.0 0.14078856078459207 0.001303597785042519 60-61 0.0 0.0 0.0 0.19528619040928624 0.001303597785042519 62-63 0.0 0.0 0.0 0.236096043291034 0.0013760198842115478 64-65 0.0 0.0 0.0 0.2760730420323379 0.0013760198842115478 66-67 0.0 0.0 0.0 0.35577356216785416 0.0014122309337960622 68-69 0.0 0.0 0.0 0.4535796070956276 0.0014484419833805766 70-71 0.0 0.0 0.0 0.5241911537854307 0.0014484419833805766 72-73 0.0 0.0 0.0 0.5905297966242611 0.0017743414296412065 74-75 0.0 0.0 0.0 0.7270816646074649 0.001810552479225721 76-77 0.0 0.0 0.0 0.8245618100889778 0.001810552479225721 78-79 0.0 0.0 0.0 0.8993014164314155 0.0018467635288102354 80-81 0.0 0.0 0.0 0.9924000249132021 0.001991607727148293 82-83 0.0 0.0 0.0 1.1595140187457362 0.002172662975070865 84-85 0.0 0.0 0.0 1.336187729668582 0.002172662975070865 86-87 0.0 0.0 0.0 1.5655847287864808 0.0022088740246553793 88-89 0.0 0.0 0.0 1.843721800645136 0.0022450850742398937 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGCTAA 70 0.008370271 27.157381 9 ATACCGA 110 9.455218E-5 25.918255 6 TACCTGG 1025 0.0 23.176407 2 GTACATG 9280 0.0 22.885454 1 TACATGG 9210 0.0 22.646696 2 GAGTACT 5790 0.0 22.283617 12-13 ACATGGG 9250 0.0 21.77752 3 GTATAGG 380 0.0 21.255205 1 AGTACTT 5920 0.0 21.232367 12-13 GTGTAGC 845 0.0 20.803911 1 ACCGCGC 140 4.838488E-4 20.365822 8 GAGTACG 210 8.590032E-7 20.36213 1 GTACTTT 6435 0.0 19.866879 14-15 CATGGGG 3095 0.0 19.495182 4 GTACCTG 1300 0.0 19.370127 1 ATGGGAT 2040 0.0 18.399115 5 TAGCCCT 860 0.0 18.230549 4 CATGGGA 6165 0.0 18.110023 4 ACTTTTT 7305 0.0 17.98744 16-17 ATAGTAC 405 2.7284841E-11 17.596264 3 >>END_MODULE