##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936515_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1380794 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.87662388451862 31.0 31.0 34.0 30.0 34.0 2 31.972011755555137 31.0 31.0 34.0 30.0 34.0 3 32.09976289004732 33.0 31.0 34.0 30.0 34.0 4 35.831669314901426 37.0 35.0 37.0 35.0 37.0 5 35.70044843763805 37.0 35.0 37.0 33.0 37.0 6 35.7647375350704 37.0 35.0 37.0 33.0 37.0 7 35.69780865212334 37.0 35.0 37.0 33.0 37.0 8 35.675224544718475 37.0 35.0 37.0 33.0 37.0 9 37.25729109483384 39.0 37.0 39.0 34.0 39.0 10-11 37.15685504137474 39.0 37.0 39.0 33.0 39.0 12-13 37.03223724900311 39.0 37.0 39.0 33.0 39.0 14-15 38.0466963211022 40.0 37.0 41.0 33.0 41.0 16-17 38.051339301879935 40.0 37.0 41.0 33.0 41.0 18-19 38.25980957333245 40.0 38.0 41.0 33.5 41.0 20-21 38.27836918468649 40.0 38.0 41.0 33.5 41.0 22-23 38.17726395103107 40.0 37.5 41.0 33.0 41.0 24-25 38.04461527208259 40.0 37.0 41.0 33.0 41.0 26-27 37.80915871592721 40.0 37.0 41.0 33.0 41.0 28-29 37.80754841055219 40.0 37.0 41.0 33.0 41.0 30-31 37.66924501410058 40.0 37.0 41.0 33.0 41.0 32-33 37.44342928778659 39.0 36.0 41.0 32.0 41.0 34-35 37.24266690034864 39.0 36.0 41.0 32.0 41.0 36-37 37.02478211811465 39.0 35.5 41.0 31.0 41.0 38-39 36.79229631646719 39.0 35.0 41.0 31.0 41.0 40-41 36.566158673922395 39.0 35.0 40.0 30.0 41.0 42-43 36.28885916364063 39.0 35.0 40.0 30.0 41.0 44-45 35.95895332685397 38.5 35.0 40.0 29.5 41.0 46-47 35.51237766096898 38.0 35.0 40.0 27.5 41.0 48-49 35.05530115281498 38.0 34.0 40.0 26.0 41.0 50-51 34.732718638696284 38.0 34.0 40.0 25.5 41.0 52-53 34.452472273199334 38.0 34.0 40.0 24.0 41.0 54-55 34.134854656089175 38.0 33.0 40.0 23.5 41.0 56-57 33.78019096259109 37.5 33.0 40.0 23.0 41.0 58-59 33.42964917286721 37.0 32.5 40.0 22.0 41.0 60-61 33.01054031231306 36.0 32.0 39.0 21.5 40.0 62-63 32.677792994465506 36.0 31.5 39.0 20.0 40.0 64-65 32.346357240833896 35.5 31.0 39.0 19.0 40.0 66-67 31.867052579892437 35.0 31.0 38.0 18.0 40.0 68-69 31.421456060788216 35.0 30.5 38.0 14.5 40.0 70-71 30.88707258287623 34.5 30.0 37.0 10.5 39.0 72-73 30.30332584006014 34.0 29.0 36.5 7.5 39.0 74-75 29.77073227432912 34.0 29.0 36.0 7.0 38.5 76-77 28.37916590019945 32.0 27.0 34.5 6.0 36.5 78-79 29.139928186246465 33.5 29.0 35.0 2.0 37.0 80-81 28.97924346426766 34.0 29.0 35.0 2.0 37.0 82-83 28.568174543052763 33.5 28.0 35.0 2.0 36.0 84-85 28.114544964708713 33.0 27.0 35.0 2.0 36.0 86-87 27.69574751918099 33.0 26.0 35.0 2.0 35.5 88-89 27.142636048534392 32.5 25.5 34.5 2.0 35.0 90-91 26.514512664452482 32.0 25.0 34.0 2.0 35.0 92-93 25.95324900021292 32.0 24.0 34.0 2.0 35.0 94-95 25.188201498558076 31.0 21.0 34.0 2.0 35.0 96-97 24.31619343653 31.0 14.5 34.0 2.0 35.0 98-99 23.199390350769193 31.0 2.0 34.0 2.0 35.0 100-101 19.399387236618928 25.0 2.0 32.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2756938570057095 1101 2 0.4002924561936716 1101 3 0.2402358075058686 1101 4 0.20656649322278753 1101 5 0.1701018815978017 1101 6 0.21865068377050534 1101 7 0.28632969502093175 1101 8 0.3882766595777838 1101 9 0.30092794332052364 1101 10-11 0.3694199103293485 1101 12-13 0.5203291710472229 1101 14-15 0.5788240293153564 1101 16-17 0.43901695242413297 1101 18-19 0.4771291536709512 1101 20-21 0.3407104846107032 1101 22-23 0.3811123312283655 1101 24-25 0.47406857518508616 1101 26-27 0.3100870215389193 1101 28-29 0.34793039003223925 1101 30-31 0.6051040129823377 1101 32-33 0.3256969219789383 1101 34-35 0.38685060703973306 1101 36-37 0.5036813268138189 1101 38-39 0.42043767441094815 1101 40-41 0.5350833699754745 1101 42-43 0.5620974842154283 1101 44-45 0.4301212553058775 1101 46-47 0.440942275503275 1101 48-49 0.39549711895323014 1101 50-51 0.4847846984702713 1101 52-53 0.49206092875105156 1101 54-55 0.6843512325602958 1101 56-57 0.5498361042146271 1101 58-59 0.40860645366574744 1101 60-61 0.4647267797709773 1101 62-63 0.41138996443381615 1101 64-65 0.5948913395412987 1101 66-67 0.47041013564202316 1101 68-69 0.5919489199331274 1101 70-71 0.5573388039165827 1101 72-73 0.5523422138545051 1101 74-75 0.5364755255150548 1101 76-77 0.7660416768351084 1101 78-79 0.8309083144911114 1101 80-81 0.7105270737161042 1101 82-83 0.5771280087960129 1101 84-85 0.34176949673612 1101 86-87 0.4497962253137935 1101 88-89 0.36604370903225103 1101 90-91 0.5524728236758065 1101 92-93 0.6193777123896176 1101 94-95 0.5650146082889904 1101 96-97 0.6008422043045449 1101 98-99 0.17637129534058005 1101 100-101 0.7117933805820407 1102 1 0.2922939423332487 1102 2 0.37780994187545147 1102 3 0.26813532786165695 1102 4 0.22788545015824013 1102 5 0.1601857870135177 1102 6 0.2249597504682015 1102 7 0.21347807444406897 1102 8 0.1794827514471038 1102 9 0.2866679011738924 1102 10-11 0.3113325400983129 1102 12-13 0.4013683570741975 1102 14-15 0.36602231242179073 1102 16-17 0.43087851495241836 1102 18-19 0.4144328033626081 1102 20-21 0.3400382070117658 1102 22-23 0.343180360550015 1102 24-25 0.3419272417483299 1102 26-27 0.3201841139383035 1102 28-29 0.3703288696505922 1102 30-31 0.5288586833723841 1102 32-33 0.4494089285982952 1102 34-35 0.48749832064947896 1102 36-37 0.5982009715409617 1102 38-39 0.5086482524404019 1102 40-41 0.37081492435884655 1102 42-43 0.46148855585177984 1102 44-45 0.4464045944569932 1102 46-47 0.41483980488310124 1102 48-49 0.440830607427543 1102 50-51 0.21006233619022652 1102 52-53 0.24878693684424036 1102 54-55 0.22047968929535244 1102 56-57 0.29378237372036864 1102 58-59 0.30306533484067444 1102 60-61 0.11753418516729397 1102 62-63 -0.13833272105662076 1102 64-65 0.04752808985481494 1102 66-67 0.21659302100576738 1102 68-69 0.2908655187256137 1102 70-71 0.024397201568781668 1102 72-73 -0.11440202330434701 1102 74-75 0.1896837117876622 1102 76-77 0.168774743843084 1102 78-79 0.14453227860183304 1102 80-81 0.05546365983874679 1102 82-83 0.23116361365192617 1102 84-85 0.02025919925328168 1102 86-87 0.3026902510930576 1102 88-89 0.16901465888292577 1102 90-91 0.059202063872835 1102 92-93 0.2695089358055647 1102 94-95 0.2372792788083231 1102 96-97 0.2681358991163698 1102 98-99 0.10090813486015904 1102 100-101 0.05261226641120231 1103 1 0.32703583364490996 1103 2 0.39879996990655187 1103 3 0.32139388270856983 1103 4 0.28059280424841404 1103 5 0.3413185857867944 1103 6 0.18015709170965977 1103 7 0.26513392375075995 1103 8 0.20192604355588628 1103 9 0.17113936299836752 1103 10-11 0.35143077078720353 1103 12-13 0.31152344590793746 1103 14-15 0.45034438692919565 1103 16-17 0.39706900841786563 1103 18-19 0.3940389168179266 1103 20-21 0.35545964547247877 1103 22-23 0.37172530425769423 1103 24-25 0.45207724090907675 1103 26-27 0.4240870809469328 1103 28-29 0.45681351372813594 1103 30-31 0.537374951645802 1103 32-33 0.5022113079662702 1103 34-35 0.4830546029573952 1103 36-37 0.4335308283749697 1103 38-39 0.2358082094922267 1103 40-41 0.33895817374686743 1103 42-43 0.5815314715413464 1103 44-45 0.39294949159957326 1103 46-47 0.4139152711973182 1103 48-49 0.45595262150861515 1103 50-51 0.5210126593573818 1103 52-53 0.6258797264561053 1103 54-55 0.5390666877798296 1103 56-57 0.5620674300614255 1103 58-59 0.29134988221443336 1103 60-61 0.2001671921298609 1103 62-63 0.3813659378145431 1103 64-65 0.537155715300365 1103 66-67 0.35629182065511955 1103 68-69 0.39822134155518185 1103 70-71 0.3203792837540078 1103 72-73 0.4063243163490249 1103 74-75 0.512327287943517 1103 76-77 0.30487882657301313 1103 78-79 0.26668740858612594 1103 80-81 0.4439383660590259 1103 82-83 0.564546399836388 1103 84-85 0.7758736770988577 1103 86-87 0.7476248623313815 1103 88-89 0.762947822813171 1103 90-91 0.6416907731913248 1103 92-93 0.6090816306845426 1103 94-95 0.476287615584404 1103 96-97 0.6330735749875842 1103 98-99 0.5926704449652718 1103 100-101 0.5181947063896111 1104 1 0.2165223176526787 1104 2 0.24061389480985795 1104 3 0.14473881538424394 1104 4 0.1579758787663721 1104 5 0.10554166000958531 1104 6 0.1971391824796953 1104 7 0.11936645384457023 1104 8 0.20892623435997137 1104 9 0.23597374821389394 1104 10-11 0.18413142869234633 1104 12-13 0.2690476627414995 1104 14-15 0.19631404591696366 1104 16-17 0.15208631255642047 1104 18-19 0.15331821499621867 1104 20-21 0.156945914430338 1104 22-23 0.1664295948196326 1104 24-25 0.06490491530242792 1104 26-27 0.011403932742759082 1104 28-29 0.07883088217082701 1104 30-31 0.20216458750516608 1104 32-33 0.19572697340343126 1104 34-35 0.1846338493761266 1104 36-37 -0.030348956617288536 1104 38-39 0.05143769958147715 1104 40-41 -0.06977158757935342 1104 42-43 0.04479219741912033 1104 44-45 0.049195373762771055 1104 46-47 0.10869619964367416 1104 48-49 0.14115328732000165 1104 50-51 -0.03787069398753573 1104 52-53 0.061782203430475136 1104 54-55 -0.026276859721434676 1104 56-57 0.07113721270290085 1104 58-59 0.2935683750308158 1104 60-61 0.14912499069345841 1104 62-63 0.020018312798335103 1104 64-65 -0.012339497203733174 1104 66-67 0.2123448958161127 1104 68-69 0.22399270133473337 1104 70-71 0.12810410841980868 1104 72-73 0.23221137087155697 1104 74-75 0.35725232091256487 1104 76-77 0.3158861667324224 1104 78-79 -0.03554916318098478 1104 80-81 -0.002029026471358719 1104 82-83 0.05920467604269675 1104 84-85 0.06751139811798268 1104 86-87 0.1678395011160525 1104 88-89 0.19505345659371542 1104 90-91 0.2721475328741043 1104 92-93 0.14878838272461792 1104 94-95 0.3256477893953331 1104 96-97 0.3212504155881639 1104 98-99 0.0519796287559906 1104 100-101 0.01994277752896423 1105 1 0.1999395980827181 1105 2 0.3789236897130017 1105 3 0.2761445746060005 1105 4 0.0817278548392224 1105 5 0.16054486418518366 1105 6 0.1778613019554527 1105 7 0.2157400577571238 1105 8 0.24079568568069476 1105 9 0.31514403953220693 1105 10-11 0.2031209827836662 1105 12-13 0.27769720887422267 1105 14-15 0.35727918902857425 1105 16-17 0.3452943983355041 1105 18-19 0.29458014672407273 1105 20-21 0.2803175365923707 1105 22-23 0.15831597050700452 1105 24-25 0.2314245387797058 1105 26-27 0.40372610593412617 1105 28-29 0.3231398910882106 1105 30-31 0.3631156598918821 1105 32-33 0.2812994445485728 1105 34-35 0.2059087854160495 1105 36-37 0.26902990727781884 1105 38-39 0.27771421055516754 1105 40-41 0.311235501103738 1105 42-43 0.37586006175956044 1105 44-45 0.4181475540339292 1105 46-47 0.43122041857032656 1105 48-49 0.6083773850903071 1105 50-51 0.5375787545881607 1105 52-53 0.4964530620211818 1105 54-55 0.5190521039523972 1105 56-57 0.7512930965863305 1105 58-59 0.611961725354341 1105 60-61 0.5088793602706687 1105 62-63 0.5388434615777342 1105 64-65 0.2995561404765823 1105 66-67 0.3415906753392086 1105 68-69 0.3553325503188276 1105 70-71 0.2576791922763917 1105 72-73 0.3997102674304962 1105 74-75 0.514411703222553 1105 76-77 0.32376700694724647 1105 78-79 0.31354478087775917 1105 80-81 0.624725741035828 1105 82-83 0.4580151813940141 1105 84-85 0.5535636069255645 1105 86-87 0.5500021172242171 1105 88-89 0.5649078912418659 1105 90-91 0.6024995182287007 1105 92-93 0.6540942802838146 1105 94-95 0.565887890270087 1105 96-97 0.794855957380193 1105 98-99 0.782204829473649 1105 100-101 0.550055363076499 1106 1 0.07325002831974103 1106 2 0.1347600187416056 1106 3 0.1691656441581415 1106 4 0.12365778616313605 1106 5 0.14175771641752988 1106 6 0.1267889466061476 1106 7 0.139828767966236 1106 8 0.12353170607245545 1106 9 0.21050870011467282 1106 10-11 0.19502086624444104 1106 12-13 0.26986958637404257 1106 14-15 0.1910843868583072 1106 16-17 0.17047441278314324 1106 18-19 0.045881548847013676 1106 20-21 0.15325776387469148 1106 22-23 0.037506485342866824 1106 24-25 0.12568499375225883 1106 26-27 0.10218311140251046 1106 28-29 0.1317565842295707 1106 30-31 0.09373123080365531 1106 32-33 0.026290321097150127 1106 34-35 0.12903819328579402 1106 36-37 0.033433553243312986 1106 38-39 0.17482363932322897 1106 40-41 -0.0076415768115367655 1106 42-43 0.042910054099372985 1106 44-45 -0.006166930174458685 1106 46-47 -0.15843367763880423 1106 48-49 -0.09266013181309773 1106 50-51 -0.2749275597319354 1106 52-53 -0.16386141839269186 1106 54-55 -0.07171378690863861 1106 56-57 -0.09598668245870812 1106 58-59 -0.17088447844083987 1106 60-61 -0.14098216342872405 1106 62-63 -0.05526445365076427 1106 64-65 0.028234608419758445 1106 66-67 -0.2335747539183508 1106 68-69 -0.24075752781510218 1106 70-71 -0.07291987246904341 1106 72-73 -0.07139658960096185 1106 74-75 -0.006545910554972778 1106 76-77 -0.1014975304555854 1106 78-79 -0.15236737690980462 1106 80-81 -0.21174463347864858 1106 82-83 0.013553925113487963 1106 84-85 -0.041287560103082654 1106 86-87 0.011385039887610304 1106 88-89 -0.12884106230039194 1106 90-91 -0.012798188030789248 1106 92-93 0.01156495693702908 1106 94-95 -0.007846055941627128 1106 96-97 -0.0041010710924709315 1106 98-99 0.12125950994414936 1106 100-101 -0.005826073320449154 1107 1 0.01640458705564285 1107 2 0.12257828616113287 1107 3 0.04914195914087571 1107 4 0.03292354596178626 1107 5 0.03672754321733862 1107 6 0.0940913976508142 1107 7 -0.04643504187797731 1107 8 -0.06468812411026903 1107 9 -0.08437489754425798 1107 10-11 0.009850318722051554 1107 12-13 0.03483638488854979 1107 14-15 0.08089726309008682 1107 16-17 0.19090413999949618 1107 18-19 0.07138447051240604 1107 20-21 -0.0687923059531812 1107 22-23 -0.07490355848561592 1107 24-25 0.04058971008498702 1107 26-27 0.14303999949173374 1107 28-29 0.09454553700744839 1107 30-31 0.027314494884592477 1107 32-33 -0.008667476550861863 1107 34-35 -0.030400794697655442 1107 36-37 -0.016207928572740116 1107 38-39 0.2377891098716347 1107 40-41 0.13539777643257622 1107 42-43 0.17635128273398237 1107 44-45 0.10038438479661238 1107 46-47 0.24303973014337998 1107 48-49 0.3131608951255771 1107 50-51 -0.022434823650499425 1107 52-53 -0.03814698336388034 1107 54-55 -0.07646875476635273 1107 56-57 -0.2044716205888335 1107 58-59 -0.23203414432625635 1107 60-61 -0.1665513434166499 1107 62-63 -0.341246572302623 1107 64-65 -0.33602238614939495 1107 66-67 -0.3467773312737741 1107 68-69 -0.21059809255998374 1107 70-71 -0.30637570593108165 1107 72-73 -0.19919792848568463 1107 74-75 -0.09972914234664998 1107 76-77 -0.12125254264104868 1107 78-79 -0.15364378283441837 1107 80-81 0.0631970755287945 1107 82-83 0.07511700560288759 1107 84-85 0.046148928728424465 1107 86-87 0.39861454145238184 1107 88-89 0.2958197254177435 1107 90-91 0.18548100867570483 1107 92-93 0.22228695334694848 1107 94-95 0.3051265303773363 1107 96-97 0.6451875618256899 1107 98-99 0.9804986031010188 1107 100-101 0.7917666498359459 1108 1 -0.0023451799358014114 1108 2 0.10736242473367597 1108 3 -0.05353737459963526 1108 4 0.04327579966656714 1108 5 0.14786054242982516 1108 6 -0.023393702458868404 1108 7 0.06368399615223552 1108 8 0.037090933610038235 1108 9 -0.02446649924852551 1108 10-11 -0.04753882558767941 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-0.9375495911266434 2316 68-69 -1.2138269742769516 2316 70-71 -0.8530879746695774 2316 72-73 -0.7173471685017567 2316 74-75 -1.1492998898218865 2316 76-77 -1.2002490744142342 2316 78-79 -1.475480708368238 2316 80-81 -1.0978561137319467 2316 82-83 -1.1514868848428428 2316 84-85 -1.1849789888275666 2316 86-87 -1.3940706069061584 2316 88-89 -1.403484908768835 2316 90-91 -1.7060400015731254 2316 92-93 -1.6075482982892524 2316 94-95 -2.0855003513659476 2316 96-97 -1.8286360292008403 2316 98-99 -1.2841781089754356 2316 100-101 -0.8918937715935833 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 9.0 4 16.0 5 43.0 6 118.0 7 273.0 8 617.0 9 1180.0 10 2016.0 11 3197.0 12 4630.0 13 6345.0 14 8077.0 15 10912.0 16 13929.0 17 17643.0 18 15771.0 19 10563.0 20 9738.0 21 10144.0 22 11058.0 23 12332.0 24 14205.0 25 16803.0 26 20744.0 27 25093.0 28 30617.0 29 37881.0 30 46002.0 31 56049.0 32 70072.0 33 87670.0 34 114276.0 35 153768.0 36 206543.0 37 240932.0 38 117254.0 39 4270.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.862780035201325 19.143362088321997 14.786655367477167 21.207202508999515 2 15.438134473647617 22.028186274509803 38.879400755574096 23.654278496268482 3 21.007239680051914 25.927484421216697 29.83528931198415 23.22998658674724 4 12.803478611017969 17.056578849216493 37.88978846029892 32.25015407946662 5 12.960591689577353 38.76350378497078 33.69587260017208 14.58003192527978 6 29.987456492423924 37.067078796692336 17.468137897470587 15.477326813413153 7 27.533500822357105 33.915513399242606 20.744329139831123 17.806656638569162 8 26.306069461494168 32.800078217509856 19.767882297132385 21.125970023863584 9 26.472087798759265 17.574091428554876 20.850756883358414 35.103063889327444 10-11 24.95140477145758 26.4939954837579 26.790455346706317 21.76414439807821 12-13 27.87084822210989 24.011691823689848 27.042954995459134 21.07450495874113 14-15 23.80521638620392 25.21850818756163 27.497902459002443 23.478372967232012 16-17 21.248660594817213 28.43000890462276 28.14480024711324 22.176530253446785 18-19 21.03604473009816 27.707754474766084 30.53414741986449 20.722053375271273 20-21 22.708011657111737 25.699890207620573 30.0255723543368 21.56652578093089 22-23 21.831547699014006 25.7539080025652 30.085635852268467 22.328908446152326 24-25 22.430412822841483 25.85654112947263 30.433282203035084 21.2797638446508 26-27 22.318125279504343 25.099607652851535 31.39867141514746 21.183595652496663 28-29 21.280372481475325 26.704065452220043 30.970308014920406 21.04525405138422 30-31 21.695283259365695 26.124071541964568 30.417900265356497 21.76274493331325 32-33 21.558154963056094 26.15836267745893 30.480169358214642 21.80331300127033 34-35 22.68059454822439 25.578631490400895 30.20297592772778 21.537798033646936 36-37 22.637298650433287 25.27878335125199 30.798648441655835 21.285269556658882 38-39 22.186467813246104 25.725140334848128 30.927189562223354 21.161202289682414 40-41 21.883946398917146 26.072228060252336 30.64463681377296 21.399188727057567 42-43 21.43256247146068 26.62672761331445 29.623460234702293 22.31724968052258 44-45 22.042397275924866 26.09758420482565 29.31422647006954 22.54579204917994 46-47 22.80734852555848 26.75254237779133 28.567367760868024 21.872741335782166 48-49 22.797785428134507 26.50816015921626 28.607578343431005 22.086476069218225 50-51 22.269088012167078 26.93375097318535 29.180460248773333 21.616700765874235 52-53 22.55285587959875 27.474302623945977 28.15250477446492 21.820336721990348 54-55 21.738412508505103 26.818010144203836 28.603547900348243 22.84002944694282 56-57 22.717690589155154 26.920286981497437 27.848113184656608 22.5139092446908 58-59 23.364689037525814 26.39821490842606 27.906105979310812 22.33099007473731 60-61 22.538685609083785 26.65106399004596 29.045349398079008 21.764901002791245 62-63 22.742025486143977 26.545863797486874 28.83364915506672 21.87846156130243 64-65 22.35995312699167 27.443342468451977 28.455508302721995 21.741196101834362 66-67 22.27368671102084 26.889847365202048 28.06443751518617 22.772028408590945 68-69 22.196069371362313 27.19425335763376 28.159981575517744 22.449695695486184 70-71 22.822311386005953 27.077053442744052 27.66130959709719 22.439325574152804 72-73 22.615464783254687 26.477115986779708 28.29401853259427 22.613400697371333 74-75 22.100423879959223 28.063207183325815 27.59561743249336 22.2407515042216 76-77 22.573484573055826 26.794764645131757 27.860767139399435 22.77098364241298 78-79 22.3300939231001 26.88628409854683 28.324354821887965 22.459267156465106 80-81 22.738783224111728 27.277494096529036 27.673617812856804 22.310104866502435 82-83 22.46249412909863 27.05333077916684 28.074912882522945 22.409262209211583 84-85 22.21246513702931 27.249788703781896 27.83098909805665 22.706757061132144 86-87 22.41771954340317 27.442015584663892 27.438503078512475 22.70176179342046 88-89 22.44947990693548 27.158073890332336 27.158436009089183 23.234010193643005 90-91 22.321814313521344 26.685039156217467 27.708152641347155 23.284993888914038 92-93 22.513431028464076 27.41617285577922 27.709019248847373 22.361376866909335 94-95 22.133082172038247 27.188898207252972 27.64389268141479 23.034126939293998 96-97 21.492825489158026 26.95777275489194 28.136010446186283 23.41339130976375 98-99 21.931718353598434 27.301251972615496 27.22057299455888 23.54645667922719 100-101 21.748137719158166 27.081190049150237 27.479483315969787 23.69118891572181 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 838.0 1 634.5 2 982.0 3 1263.0 4 769.5 5 601.0 6 901.5 7 1651.0 8 2462.0 9 2797.0 10 2412.0 11 1862.0 12 1780.5 13 1865.0 14 1941.0 15 2042.0 16 2145.5 17 2303.5 18 2539.5 19 2719.5 20 2977.0 21 3319.0 22 3746.0 23 4441.5 24 5192.0 25 6031.5 26 7396.5 27 9185.0 28 10932.0 29 13074.0 30 15550.5 31 18131.0 32 21580.5 33 24237.5 34 26279.5 35 28482.0 36 31232.5 37 34079.5 38 35276.0 39 36180.5 40 36943.5 41 37910.5 42 39039.5 43 40350.5 44 42412.0 45 44647.0 46 47080.5 47 57337.0 48 85858.0 49 84745.5 50 57000.0 51 46835.5 52 43032.5 53 42124.5 54 40881.5 55 38005.0 56 34459.0 57 30854.0 58 28790.0 59 26421.0 60 22237.5 61 19049.5 62 16132.5 63 12052.5 64 8255.5 65 6009.5 66 4589.0 67 3580.0 68 2904.0 69 2283.5 70 1705.0 71 1283.5 72 1067.5 73 978.5 74 786.5 75 579.0 76 400.0 77 242.0 78 169.0 79 119.0 80 83.5 81 58.0 82 35.0 83 18.5 84 11.5 85 10.0 86 7.5 87 2.5 88 1.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01187722426372073 2 0.008835496098621517 3 0.005069546941832018 4 9.414872891973748E-4 5 0.005069546941832018 6 0.0 7 5.069546941832019E-4 8 0.0021002408759018365 9 0.0 10-11 0.0 12-13 0.0 14-15 7.604320412748027E-4 16-17 0.0036573160080359563 18-19 0.0017019193304721775 20-21 4.34532595014173E-4 22-23 3.9832154542965856E-4 24-25 5.793767933522306E-4 26-27 1.0863314875354324E-4 28-29 3.6211049584514415E-5 30-31 7.242209916902884E-4 32-33 0.003765949156789499 34-35 0.0010501204379509182 36-37 0.0012673867354580045 38-39 0.0056851347847687635 40-41 7.242209916902883E-5 42-43 0.0017019193304721775 44-45 0.004960913793078475 46-47 0.0 48-49 0.0022088740246553793 50-51 0.001376019884211548 52-53 7.966430908593171E-4 54-55 3.9832154542965856E-4 56-57 0.010682259627431754 58-59 0.0017743414296412063 60-61 0.003476260760113384 62-63 3.2589944626062973E-4 64-65 0.003041728165099211 66-67 0.0010501204379509182 68-69 0.0016294972313031487 70-71 0.004635014346817845 72-73 0.0027158287188385815 74-75 0.0069887325698112825 76-77 0.0020278187767328077 78-79 0.006517988925212595 80-81 0.0027882508180076104 82-83 0.0031503613138527545 84-85 0.0022450850742398937 86-87 9.776983387818894E-4 88-89 0.002281296123824408 90-91 0.007712953561501571 92-93 0.002607195570085038 94-95 5.793767933522306E-4 96-97 2.172662975070865E-4 98-99 9.414872891973748E-4 100-101 0.0019916077271482932 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1380794.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.869892261262144 #Duplication Level Percentage of deduplicated Percentage of total 1 76.85867938899446 41.40368778028021 2 13.495651262917614 14.540185590578764 3 4.347713573581059 7.026325853749158 4 1.8763998625425544 4.04325833736858 5 0.9380717070182858 2.526691089520666 6 0.5706518030760294 1.8444570690240407 7 0.35815236157441555 1.3505540391791258 8 0.25037294695037526 1.0790050941881133 9 0.19148091064685502 0.9283550423970999 >10 0.9905226277807047 9.68088580826684 >50 0.07568348038385234 2.8141141281743427 >100 0.03878252692213516 4.07373388604039 >500 0.0032431231496723553 1.2562867235381459 >1k 0.0037836436746177472 4.271141917770145 >5k 6.756506561817406E-4 2.283489293699623 >10k+ 1.3513013123634814E-4 0.8778283462247363 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 12059 0.8733380938793188 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8110 0.5873432242608239 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6760 0.4895733903826349 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5732 0.41512347243687325 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5654 0.4094745487016891 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 5113 0.37029419305124445 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4260 0.3085181424600628 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4179 0.3026519524273715 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4032 0.29200590384952424 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3345 0.24225192172040144 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3081 0.22313248753977782 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2795 0.2024197671774356 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2686 0.19452575836801145 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2650 0.1919185627979264 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2356 0.17062646564223194 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2115 0.15317273974249598 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2089 0.15128976516410125 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1982 0.14354060055301515 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1973 0.1428888016604939 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1855 0.13434299395854848 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1828 0.13238759728098473 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1807 0.1308667331984351 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1730 0.1252902315624199 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1504 0.10892283715021936 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1457 0.105518998489275 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1439 0.10421540070423248 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1432 0.10370844601004928 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 7.242209916902883E-5 0.0 5 0.0 0.0 0.0 7.242209916902883E-5 0.0 6 0.0 0.0 0.0 7.242209916902883E-5 0.0 7 0.0 0.0 0.0 7.242209916902883E-5 0.0 8 0.0 0.0 0.0 7.242209916902883E-5 1.4484419833805766E-4 9 0.0 0.0 0.0 7.242209916902883E-5 1.4484419833805766E-4 10-11 0.0 0.0 0.0 7.242209916902883E-5 1.4484419833805766E-4 12-13 0.0 0.0 0.0 7.242209916902883E-5 3.6211049584514415E-4 14-15 0.0 0.0 0.0 7.242209916902883E-5 6.517988925212595E-4 16-17 0.0 0.0 0.0 1.4484419833805766E-4 8.690651900283461E-4 18-19 0.0 0.0 0.0 2.1726629750708652E-4 8.690651900283461E-4 20-21 0.0 0.0 0.0 3.2589944626062973E-4 0.001050120437950918 22-23 0.0 0.0 0.0 4.34532595014173E-4 0.0011587535867044613 24-25 0.0 0.0 0.0 7.966430908593171E-4 0.00123117568587349 26-27 0.0 0.0 0.0 0.0011225425371199469 0.001303597785042519 28-29 0.0 0.0 0.0 0.0021364519254863505 0.001303597785042519 30-31 0.0 0.0 0.0 0.0044539590988952734 0.001303597785042519 32-33 0.0 0.0 0.0 0.007314632016071913 0.0013760198842115478 34-35 3.6211049584514415E-5 0.0 0.0 0.010428782280340151 0.0015570751321341198 36-37 7.242209916902883E-5 0.0 0.0 0.012456601057072959 0.0015932861817186342 38-39 7.242209916902883E-5 0.0 0.0 0.014194731437129652 0.0016294972313031487 40-41 7.242209916902883E-5 0.0 0.0 0.016765715957630177 0.0016657082808876633 42-43 7.242209916902883E-5 0.0 0.0 0.02031439881691259 0.0016657082808876633 44-45 7.242209916902883E-5 0.0 0.0 0.023645815378687915 0.0016657082808876633 46-47 7.242209916902883E-5 0.0 0.0 0.02730313138672387 0.0016657082808876633 48-49 7.242209916902883E-5 0.0 0.0 0.03157603523769657 0.0017381303800566921 50-51 7.242209916902883E-5 0.0 0.0 0.03965109929504329 0.0018829745783947498 52-53 7.242209916902883E-5 0.0 0.0 0.05536669481472255 0.001991607727148293 54-55 7.242209916902883E-5 0.0 0.0 0.07698469141667766 0.0020278187767328072 56-57 7.242209916902883E-5 0.0 0.0 0.10432403385298603 0.0020278187767328072 58-59 7.242209916902883E-5 0.0 0.0 0.14129551547877525 0.0020278187767328072 60-61 7.242209916902883E-5 0.0 0.0 0.19572072300430043 0.0020278187767328072 62-63 7.242209916902883E-5 0.0 0.0 0.23674784218355527 0.002100240875901836 64-65 7.242209916902883E-5 0.0 0.0 0.27668862987527465 0.0021364519254863505 66-67 7.242209916902883E-5 0.0 0.0 0.356606416308298 0.002172662975070865 68-69 7.242209916902883E-5 0.0 0.0 0.45499183802942367 0.002172662975070865 70-71 7.242209916902883E-5 0.0 0.0 0.52498779687629 0.0022450850742398937 72-73 7.242209916902883E-5 0.0 0.0 0.5909281181696908 0.00246235137174698 74-75 7.242209916902883E-5 0.0 0.0 0.7278058855991553 0.0026434066196695523 76-77 7.242209916902883E-5 0.0 0.0 0.8259740410227738 0.0027520397684230955 78-79 7.242209916902883E-5 0.0 0.0 0.900315325819782 0.0027520397684230955 80-81 7.242209916902883E-5 0.0 0.0 0.994427843689935 0.00278825081800761 82-83 7.242209916902883E-5 0.0 0.0 1.1605279281341025 0.0028244618675921243 84-85 7.242209916902883E-5 0.0 0.0 1.3361515186189976 0.0028244618675921243 86-87 7.242209916902883E-5 0.0 0.0 1.5636655431585016 0.002896883966761153 88-89 7.242209916902883E-5 0.0 0.0 1.8383987763562124 0.002969306065930182 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTACT 7735 0.0 23.333763 12-13 TACCTGG 1060 0.0 23.306915 2 TACATGG 8790 0.0 21.674187 2 GTACTTT 8275 0.0 21.669151 14-15 GTACATG 8985 0.0 21.36475 1 AGTACTT 7885 0.0 20.932188 12-13 GTACCTG 1265 0.0 20.658876 1 TAACGCA 115 0.0037229043 20.65289 4 ACATGGG 8890 0.0 20.572325 3 TAGCCCT 845 0.0 19.675238 4 ACTTTTT 9455 0.0 19.040163 16-17 GTGCTAG 525 0.0 19.006165 1 GTATAGC 540 0.0 18.478216 1 TACTTTT 9050 0.0 18.160194 14-15 CATGGGG 3045 0.0 17.627869 4 CATGGGA 5810 0.0 17.578062 4 ATAGCCC 460 0.0 17.555593 3 TAAGGTG 610 0.0 17.131742 5 GTAGCCC 670 0.0 17.016132 3 ATGGGAT 2060 0.0 16.141336 5 >>END_MODULE