FastQCFastQC Report
Thu 26 May 2016
SRR936514_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936514_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1381435
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT101680.7360462128149352No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66690.4827588703051537No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT65890.4769677907393399No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA56110.40617184304726606No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51420.3722216390926826No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT47850.3463789465302385No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA47510.34391773771476764No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC42340.30649288602069585No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT33370.24156040638900855No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT31480.22787898091477338No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31370.22708270747447398No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT27420.19848925211826832No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC25950.18784814341608544No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC23450.1697510197729173No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC20680.14969940677628696No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG18740.1356560388291885No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG18080.13087839818739208No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA17730.12834480087734856No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA17420.1261007575455957No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT16870.12211939034409872No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA16520.11958579303405516No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16510.11951340453948249No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG14470.10474615164665728No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC14060.10177822336917769No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG89950.023.7203121
TACATGG89200.023.4402942
GAGTACT58950.022.24486212-13
ACATGGG91050.022.1811583
GCGTAGC1100.002870680821.5999411
GGTATAC1553.894574E-521.4605871
GTACTTT63600.020.35442214-15
AGTACTT61000.020.29001412-13
ATGGGAG20050.020.147045
CATGGGA56750.020.0965084
TACGGGA1250.00601719419.0079462
CATGGGG33300.018.8367044
GTGTAGC7350.018.7493361
CTAGGAC7450.018.4976673
GTGCGGA1559.5988606E-418.3947871
CTATACT5800.018.0247784
ACTTTTT75550.017.67001716-17
ACTATAC3259.9717E-917.5457973
ATGGGAT20100.017.4961455
TACTTTT71050.017.28406314-15
Produced by FastQC (version 0.11.5)