##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936514_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1381435 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.465155436194973 31.0 31.0 34.0 30.0 34.0 2 31.651183008972556 31.0 31.0 34.0 30.0 34.0 3 31.92999308689877 31.0 31.0 34.0 30.0 34.0 4 35.7320547112242 37.0 35.0 37.0 35.0 37.0 5 35.5558198539924 37.0 35.0 37.0 33.0 37.0 6 35.67238197960816 37.0 35.0 37.0 33.0 37.0 7 35.60912239808605 37.0 35.0 37.0 33.0 37.0 8 35.595375822966695 37.0 35.0 37.0 33.0 37.0 9 37.20822260909851 39.0 37.0 39.0 33.0 39.0 10-11 37.09309449956024 39.0 37.0 39.0 33.0 39.0 12-13 36.964538324278735 39.0 37.0 39.0 33.0 39.0 14-15 38.011018976643854 40.0 37.0 41.0 33.0 41.0 16-17 37.94512517780424 40.0 37.0 41.0 33.0 41.0 18-19 38.06384194696095 40.0 37.0 41.0 33.0 41.0 20-21 38.15485093399255 40.0 37.0 41.0 33.0 41.0 22-23 38.06876472653437 40.0 37.0 41.0 33.0 41.0 24-25 37.94838627948474 40.0 37.0 41.0 33.0 41.0 26-27 37.70889871763782 39.5 37.0 41.0 32.5 41.0 28-29 37.61948046777445 39.0 37.0 41.0 32.0 41.0 30-31 37.58796432694987 39.0 37.0 41.0 32.5 41.0 32-33 37.38090536290162 39.0 36.0 41.0 32.0 41.0 34-35 37.18668305059594 39.0 36.0 41.0 31.0 41.0 36-37 36.92486580982819 39.0 35.0 41.0 31.0 41.0 38-39 36.749588652379586 39.0 35.0 41.0 31.0 41.0 40-41 36.51948987827875 39.0 35.0 40.5 30.0 41.0 42-43 36.264891942074726 39.0 35.0 40.0 30.0 41.0 44-45 35.94649223452424 39.0 35.0 40.0 29.5 41.0 46-47 35.49639577685522 38.0 35.0 40.0 27.5 41.0 48-49 35.000802426462336 38.0 34.0 40.0 26.0 41.0 50-51 34.791700297154776 38.0 34.0 40.0 25.5 41.0 52-53 34.502128221740435 38.0 34.0 40.0 24.0 41.0 54-55 34.2424395646556 38.0 33.0 40.0 24.0 41.0 56-57 33.96319298410711 37.5 33.0 40.0 23.0 41.0 58-59 33.61354171567971 37.0 33.0 40.0 22.5 41.0 60-61 33.20230123024247 36.5 32.0 39.5 22.0 41.0 62-63 32.90402733389555 36.0 32.0 39.0 21.0 40.0 64-65 32.44840727214817 36.0 31.0 39.0 19.5 40.0 66-67 32.0142927463109 35.0 31.0 38.5 18.0 40.0 68-69 31.563113356763076 35.0 31.0 38.0 17.5 40.0 70-71 31.069188199227614 34.5 30.0 37.0 13.5 39.0 72-73 30.635085617491956 34.0 30.0 37.0 11.0 39.0 74-75 30.279626258202523 34.0 29.0 36.0 8.0 39.0 76-77 28.83755008378968 32.5 28.0 35.0 7.0 37.0 78-79 29.620402697195306 34.0 29.0 35.0 7.0 37.0 80-81 29.46921172548835 34.0 29.0 35.0 2.0 37.0 82-83 29.12457589390742 34.0 29.0 35.0 2.0 36.5 84-85 28.66976911689656 34.0 29.0 35.0 2.0 36.0 86-87 28.302907845826983 33.0 28.0 35.0 2.0 36.0 88-89 27.85013699522598 33.0 27.0 35.0 2.0 35.0 90-91 27.354774926073247 33.0 26.0 35.0 2.0 35.0 92-93 26.919672297285068 33.0 25.5 34.5 2.0 35.0 94-95 26.372138392323926 32.0 25.0 34.0 2.0 35.0 96-97 25.64837831675034 32.0 23.5 34.0 2.0 35.0 98-99 24.784176237028888 32.0 18.5 34.0 2.0 35.0 100-101 21.954400315613835 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5332571774057548 1101 2 0.5335085033593323 1101 3 0.3461294825591459 1101 4 0.29758721921501063 1101 5 0.2986892844817035 1101 6 0.271474127499161 1101 7 0.3191732939970393 1101 8 0.3206555104425419 1101 9 0.41312967007097967 1101 10-11 0.35665021843296074 1101 12-13 0.37932183691641796 1101 14-15 0.44558907148348226 1101 16-17 0.4750545613152042 1101 18-19 0.5796479808140518 1101 20-21 0.43441764571582553 1101 22-23 0.3565899255136529 1101 24-25 0.26252723661364996 1101 26-27 0.41333526768887907 1101 28-29 0.3877729789452147 1101 30-31 0.31696639001210514 1101 32-33 0.4016234208425047 1101 34-35 0.4826479815043214 1101 36-37 0.4766925276514016 1101 38-39 0.4001294751191935 1101 40-41 0.37180686767595716 1101 42-43 0.462584213718074 1101 44-45 0.3250945827819365 1101 46-47 0.3678139812908441 1101 48-49 0.1950176723903141 1101 50-51 0.2538411334552606 1101 52-53 0.30321906894307915 1101 54-55 0.1976155836890996 1101 56-57 0.04347978087827187 1101 58-59 0.1282866720025666 1101 60-61 0.524664300111759 1101 62-63 0.32633345577005457 1101 64-65 0.36515165559741547 1101 66-67 0.33543510039830693 1101 68-69 0.08052432389091635 1101 70-71 0.0022057315235493036 1101 72-73 0.18073515598903 1101 74-75 0.1924175417369085 1101 76-77 0.513919325763748 1101 78-79 0.576577777034597 1101 80-81 0.46600468580080445 1101 82-83 0.4696710069075998 1101 84-85 0.5657599574991146 1101 86-87 0.43305081387459765 1101 88-89 0.400226167893031 1101 90-91 0.44109925047014187 1101 92-93 0.3759280086013952 1101 94-95 0.3864694014368091 1101 96-97 0.49638577976777043 1101 98-99 0.4225472915571977 1101 100-101 0.4242841489842455 1102 1 0.5001005699776115 1102 2 0.5632521801064421 1102 3 0.25783439271169684 1102 4 0.15842051720316164 1102 5 0.2110728221101823 1102 6 0.233431402244328 1102 7 0.26920651846721455 1102 8 0.10963064157316182 1102 9 0.22250038446777864 1102 10-11 0.20948278124134845 1102 12-13 0.243180682401416 1102 14-15 0.31346737095612554 1102 16-17 0.4020243482544572 1102 18-19 0.2326912009014208 1102 20-21 0.3195161173902008 1102 22-23 0.2520954322928546 1102 24-25 0.19298771363221334 1102 26-27 -0.03840724973629506 1102 28-29 0.17062915233454135 1102 30-31 0.26497928393415293 1102 32-33 0.460406698330452 1102 34-35 0.5000279139243347 1102 36-37 0.3649112719078076 1102 38-39 0.4364812869131782 1102 40-41 0.4190520642658555 1102 42-43 0.12855387341466695 1102 44-45 0.09378016618949658 1102 46-47 -0.10826298637537235 1102 48-49 0.03484320857671719 1102 50-51 0.03166698067924756 1102 52-53 0.08019945943664197 1102 54-55 0.4024338387682036 1102 56-57 0.19145167439030786 1102 58-59 0.08289356289906635 1102 60-61 0.3382227115579468 1102 62-63 0.06928491976744766 1102 64-65 0.1822021709301609 1102 66-67 0.43913362014212964 1102 68-69 0.2709782973961694 1102 70-71 0.038547646666842184 1102 72-73 0.08811664359701155 1102 74-75 -0.2871232536710444 1102 76-77 0.006434630227136751 1102 78-79 0.2241255283741772 1102 80-81 0.3140670629762994 1102 82-83 0.3998713433937233 1102 84-85 0.6540705992262197 1102 86-87 0.24595463256795824 1102 88-89 0.16470708856430605 1102 90-91 0.29663218510983214 1102 92-93 0.4116478485584132 1102 94-95 0.17319375143893367 1102 96-97 -0.1297498608989791 1102 98-99 -0.48579134355329145 1102 100-101 0.0871999850391596 1103 1 0.5218794258818029 1103 2 0.5408612152974364 1103 3 0.25463161978460747 1103 4 0.15196545479386003 1103 5 0.12308401074454878 1103 6 -0.005679301496542166 1103 7 0.11312506900175379 1103 8 0.2591263892072959 1103 9 0.09899608732630583 1103 10-11 0.10290945854880817 1103 12-13 0.20477163878207705 1103 14-15 0.15567628340927797 1103 16-17 0.21902643690489754 1103 18-19 0.1676688526051393 1103 20-21 0.21174254500779455 1103 22-23 0.17853648257059263 1103 24-25 0.21787768415933328 1103 26-27 0.27077043304696247 1103 28-29 0.1326399458900127 1103 30-31 0.1925626389968258 1103 32-33 0.11465024076587582 1103 34-35 0.19266627713880524 1103 36-37 0.07778452269599256 1103 38-39 0.24599162612791758 1103 40-41 0.22198038905231243 1103 42-43 0.4195197359640943 1103 44-45 0.3787508381660203 1103 46-47 0.41976127673662944 1103 48-49 0.2922839928346619 1103 50-51 0.10477027274000505 1103 52-53 0.20888087044775006 1103 54-55 0.25604183095090605 1103 56-57 0.012104818823253538 1103 58-59 0.12330829038680946 1103 60-61 0.24534493053173634 1103 62-63 0.08817680247643267 1103 64-65 0.11715638128595884 1103 66-67 0.04454435733300954 1103 68-69 0.047465835290822156 1103 70-71 -0.15678989557367728 1103 72-73 -0.1231594349025329 1103 74-75 -0.002252931734421537 1103 76-77 -0.0302149953697608 1103 78-79 0.01536452213456485 1103 80-81 -0.0468153852486104 1103 82-83 0.06154052539603683 1103 84-85 0.14268284509171636 1103 86-87 -0.052780324803030965 1103 88-89 0.002063115229496759 1103 90-91 -0.10484330287845012 1103 92-93 0.1107094044844672 1103 94-95 0.01856224152639996 1103 96-97 -0.1265510387608657 1103 98-99 -0.1285689062325588 1103 100-101 0.11851815261055876 1104 1 0.28436633620215446 1104 2 0.35956214320607316 1104 3 0.21558307847880442 1104 4 0.13807334557857587 1104 5 0.15624352222482685 1104 6 0.08484748328411484 1104 7 0.07014090997672895 1104 8 0.11030336167615928 1104 9 0.0913930861336496 1104 10-11 0.07251033508376281 1104 12-13 0.08332896286511726 1104 14-15 0.22349863050439467 1104 16-17 0.2791275778854754 1104 18-19 0.11546319422645723 1104 20-21 0.2039054071429689 1104 22-23 0.16258109128957443 1104 24-25 0.2517358170368169 1104 26-27 0.23909606032864872 1104 28-29 0.038518519126292006 1104 30-31 0.1612156029852443 1104 32-33 0.19072950474322425 1104 34-35 0.377076563391725 1104 36-37 0.37333698673820237 1104 38-39 0.3487738365902686 1104 40-41 0.3420243067271258 1104 42-43 0.29344054775912554 1104 44-45 0.5696586065025571 1104 46-47 0.74816637409657 1104 48-49 0.6116782380735017 1104 50-51 0.7629852874928886 1104 52-53 0.6115603335549338 1104 54-55 0.7849914286753119 1104 56-57 0.800680161579642 1104 58-59 0.6844816106266691 1104 60-61 0.5911064505864161 1104 62-63 0.5305162900429323 1104 64-65 0.6535475850212364 1104 66-67 0.8861391538917331 1104 68-69 0.8423931771500648 1104 70-71 0.7102492681416557 1104 72-73 0.975869011566548 1104 74-75 0.8992823683469595 1104 76-77 0.8382744518100651 1104 78-79 0.8115974222013982 1104 80-81 1.0354802452609881 1104 82-83 0.9021624045208938 1104 84-85 1.1679733943051254 1104 86-87 1.147988461823445 1104 88-89 1.2214453884313805 1104 90-91 1.1436567827443476 1104 92-93 1.0443956855909242 1104 94-95 1.0198344598842333 1104 96-97 1.182504822157405 1104 98-99 1.24592626024695 1104 100-101 1.0759204327531897 1105 1 0.4400940358010814 1105 2 0.5064392880241932 1105 3 0.20167846395954214 1105 4 0.1309602604454767 1105 5 -0.06017806747490084 1105 6 0.08098298551581706 1105 7 0.22721506354923093 1105 8 0.13146289558547863 1105 9 0.11171570078017368 1105 10-11 0.05489860053337736 1105 12-13 0.06489998126907892 1105 14-15 0.24104843400080256 1105 16-17 0.19349927398518219 1105 18-19 0.10698884024021282 1105 20-21 0.08418952253193623 1105 22-23 0.11181729924384598 1105 24-25 -0.04778656096021905 1105 26-27 0.08761270229575757 1105 28-29 -0.04990639687819254 1105 30-31 0.16657154625347914 1105 32-33 0.13077927302393988 1105 34-35 0.051509627094254995 1105 36-37 0.2572900920584047 1105 38-39 0.16025327960907987 1105 40-41 0.2148215648572176 1105 42-43 0.2681588343235788 1105 44-45 0.12721514226883102 1105 46-47 0.4309737306314645 1105 48-49 0.4904667792255992 1105 50-51 0.3422009343372494 1105 52-53 0.2732380288065457 1105 54-55 0.24064053655240514 1105 56-57 0.31354554872645224 1105 58-59 0.36161316821453937 1105 60-61 0.401911938017804 1105 62-63 0.25915821246056936 1105 64-65 0.21184656483463726 1105 66-67 0.21657095662942538 1105 68-69 0.15459296333006023 1105 70-71 0.25141617548939976 1105 72-73 0.10275699796211768 1105 74-75 -0.09017988066833738 1105 76-77 0.05533939587053993 1105 78-79 0.08882122062966147 1105 80-81 -0.04945273076659262 1105 82-83 -0.03164592785311626 1105 84-85 0.33309639909300515 1105 86-87 0.1406784116520079 1105 88-89 0.0887298112898165 1105 90-91 -0.0584400327365735 1105 92-93 0.047264429157987564 1105 94-95 0.2505839810721824 1105 96-97 0.19642838029317744 1105 98-99 0.14568489914324445 1105 100-101 0.40571373846769987 1106 1 0.28655289885935176 1106 2 0.2400780970417813 1106 3 0.09777246622684643 1106 4 0.07200801967696435 1106 5 -0.0920372802739422 1106 6 0.03402611694910718 1106 7 -0.052808196588287615 1106 8 -0.008844844607359903 1106 9 0.04641072133082247 1106 10-11 -0.01944204772181024 1106 12-13 0.16737109131162242 1106 14-15 0.08201563077680873 1106 16-17 0.2204096502337478 1106 18-19 0.15028964572497472 1106 20-21 0.14966294228003107 1106 22-23 0.039860442325839074 1106 24-25 0.08824377413579043 1106 26-27 -0.0038631935014308283 1106 28-29 0.1767937435120146 1106 30-31 -0.05642593622803105 1106 32-33 0.16522729439791561 1106 34-35 -0.2523911520178501 1106 36-37 -0.1800608336177234 1106 38-39 -0.07078530510101189 1106 40-41 0.03204986226826634 1106 42-43 -0.23825307978724197 1106 44-45 -0.14058652778295055 1106 46-47 -0.1727584683974115 1106 48-49 -0.14266209600790347 1106 50-51 -0.004294971608302944 1106 52-53 0.0068554042038186935 1106 54-55 -0.40184364799851835 1106 56-57 -0.2702836700507092 1106 58-59 -0.32568871640642527 1106 60-61 0.07485353000461714 1106 62-63 -0.5929812589024763 1106 64-65 -0.1795003564820874 1106 66-67 -0.18455225826451738 1106 68-69 -0.11519947069515624 1106 70-71 -0.11689985485501708 1106 72-73 -0.06269564049324572 1106 74-75 0.0033249868602389654 1106 76-77 0.09100261032649115 1106 78-79 -0.01962357917799551 1106 80-81 -0.014091450788061621 1106 82-83 0.006409697982970641 1106 84-85 0.08933136934919617 1106 86-87 0.13025957541371724 1106 88-89 -0.0034203116506361653 1106 90-91 0.09494198287176303 1106 92-93 0.33867430008566046 1106 94-95 0.4433998974922453 1106 96-97 0.4187141267958907 1106 98-99 0.24513782928222483 1106 100-101 0.4066081261674164 1107 1 0.6399961349709997 1107 2 0.5988074262249405 1107 3 0.42276650995739473 1107 4 0.20805638714676178 1107 5 -0.0334526948431062 1107 6 0.14013891460246697 1107 7 0.1911114129878584 1107 8 0.1784853376672899 1107 9 0.1636798038091385 1107 10-11 0.31275229318174524 1107 12-13 0.3868776816355677 1107 14-15 0.519241930588521 1107 16-17 0.526690504349979 1107 18-19 0.39372301876677085 1107 20-21 0.19435981299696437 1107 22-23 0.2549778384754049 1107 24-25 0.324798616646909 1107 26-27 0.33564134735345164 1107 28-29 0.26694886270145446 1107 30-31 0.3022667586956871 1107 32-33 0.20144269359606426 1107 34-35 0.2587384897093372 1107 36-37 0.44968464794345664 1107 38-39 0.4365843294530336 1107 40-41 0.464038487714987 1107 42-43 0.4071544443260393 1107 44-45 0.31628521388147846 1107 46-47 0.5151847613096479 1107 48-49 0.7703274388907175 1107 50-51 0.7699027253008452 1107 52-53 0.6763687833940253 1107 54-55 0.6878129378422102 1107 56-57 0.592008661092251 1107 58-59 0.5964353020900361 1107 60-61 0.6328519672844592 1107 62-63 0.5367332244391818 1107 64-65 0.7332583569637166 1107 66-67 0.6518914005501202 1107 68-69 0.6369213455058969 1107 70-71 0.591411254548202 1107 72-73 0.8839810173350884 1107 74-75 1.013088401275695 1107 76-77 0.8888199257782077 1107 78-79 0.9487067439976578 1107 80-81 0.7743441638605155 1107 82-83 0.6879114464362637 1107 84-85 0.6833179709452217 1107 86-87 0.8780273193325598 1107 88-89 0.9544515709147916 1107 90-91 0.7164191376963416 1107 92-93 0.9395960487951349 1107 94-95 1.1463219280037187 1107 96-97 1.008864634679938 1107 98-99 0.9252495253459649 1107 100-101 0.8959070796572739 1108 1 0.3038468652368209 1108 2 0.3554041390480691 1108 3 0.1474836218443194 1108 4 0.09846342698777022 1108 5 -0.10734539741505245 1108 6 -0.02806307548631537 1108 7 -0.005778713713588957 1108 8 0.08448724668807017 1108 9 0.11070735385555963 1108 10-11 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-2.0606290532451723 2316 76-77 -1.8507080379737388 2316 78-79 -1.6065269071824346 2316 80-81 -1.6410031006285628 2316 82-83 -1.4251256807700692 2316 84-85 -1.7559678882554302 2316 86-87 -1.8062521153059734 2316 88-89 -1.8251724231340347 2316 90-91 -1.8952127010809612 2316 92-93 -1.9433403142264396 2316 94-95 -1.9095014303515931 2316 96-97 -2.365151731046712 2316 98-99 -2.783644773700498 2316 100-101 -2.9785461562932234 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 8.0 4 22.0 5 43.0 6 124.0 7 335.0 8 685.0 9 1316.0 10 2117.0 11 3265.0 12 4833.0 13 6484.0 14 8101.0 15 10846.0 16 13840.0 17 17202.0 18 14695.0 19 9679.0 20 8830.0 21 9242.0 22 9929.0 23 11161.0 24 13174.0 25 15520.0 26 18818.0 27 23460.0 28 28917.0 29 35511.0 30 43833.0 31 53648.0 32 66260.0 33 83676.0 34 109119.0 35 145701.0 36 199955.0 37 250797.0 38 151183.0 39 9106.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.67468536868407 19.188945510704155 14.962491398870831 21.17387772174095 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20.785777108586363 20-21 22.699085624399853 25.681492680253747 30.015212447640476 21.604209247705917 22-23 21.88450563117029 25.70941597691647 30.13056149550679 22.275516896406447 24-25 22.404492429973182 25.878633449999455 30.432557449319003 21.284316670708357 26-27 22.248060893201636 25.14823353976119 31.414109241477163 21.189596325560014 28-29 21.317506795469928 26.647326873866668 30.942534393583482 21.09263193707992 30-31 21.60428163622669 26.15911214031501 30.45747011530405 21.77913610815424 32-33 21.512620128573907 26.150177593566454 30.48499530724429 21.852206970615352 34-35 22.657154294574674 25.56280489261482 30.271769305552436 21.508271507258065 36-37 22.63331289502698 25.276188600926748 30.836662702069578 21.2538358019767 38-39 22.2224997122549 25.69831573201991 30.875230105593403 21.203954450131786 40-41 21.95234598081866 26.05414311407784 30.617864773603777 21.375646131499725 42-43 21.485678543026268 26.65008327291326 29.58181106731586 22.282427116744607 44-45 22.038161968117162 26.038266210077232 29.34411175896363 22.579460062841978 46-47 22.805082064880228 26.741105264415193 28.573222808339615 21.880589862364968 48-49 22.821472977791128 26.540313867014643 28.546912639563317 22.09130051563091 50-51 22.28913765714259 26.929370575458666 29.127059417117863 21.654432350280885 52-53 22.54953272381118 27.448150802440985 28.147689679385557 21.854626794362282 54-55 21.797750450029447 26.75811926241866 28.614922842528244 22.82920744502365 56-57 22.684255828044545 26.948575374932766 27.83370409756613 22.53346469945656 58-59 23.436444127216348 26.341771689153948 27.925768625618524 22.296015558011177 60-61 22.59481771962579 26.59793493518349 29.04494190874073 21.762305436449996 62-63 22.746887372058126 26.56282915239661 28.814693037225574 21.87559043831969 64-65 22.40847483580621 27.43508720741867 28.35970557880596 21.796732377969164 66-67 22.355534481697717 26.857501710240083 28.053294338652872 22.733669469409325 68-69 22.24250314619747 27.163818349036273 28.144903522040032 22.448774982726228 70-71 22.831276983260608 27.09275002678419 27.61543985429401 22.460533135661194 72-73 22.58104796823593 26.519886929171477 28.27975981497501 22.619305287617586 74-75 22.13977571864609 28.071870218964417 27.56098823360789 22.227365828781604 76-77 22.581952839090516 26.83543992248553 27.814831048787138 22.76777618963682 78-79 22.385619850386558 26.90833878112754 28.281834484066742 22.424206884419164 80-81 22.788803518583876 27.287269568050387 27.65628648223061 22.267640431135124 82-83 22.485792060929434 27.04648620472464 28.107900700080364 22.35982103426557 84-85 22.228303362001565 27.184830452031388 27.88546462803695 22.701401557930094 86-87 22.464878436005833 27.40146216325885 27.48601960675721 22.647639793978104 88-89 22.537631808173174 27.11760152750132 27.192325710549504 23.152440953775997 90-91 22.252201732134267 26.667648955184113 27.848494908330395 23.231654404351225 92-93 22.530862745254176 27.395823869795716 27.79693911640993 22.276374268540177 94-95 22.235048020730737 27.1737285175416 27.70307323265504 22.88815022907262 96-97 21.482172726854806 26.991055142758995 28.19514130384027 23.33163082654593 98-99 22.003307015244992 27.24736589864954 27.260324649281188 23.48900243682428 100-101 21.79936269418986 27.01317289318232 27.587928184185216 23.599536228442606 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 915.0 1 701.0 2 1055.0 3 1312.5 4 792.0 5 622.5 6 939.0 7 1712.5 8 2532.5 9 2920.0 10 2488.0 11 1876.0 12 1765.0 13 1835.0 14 1978.0 15 2097.0 16 2236.0 17 2375.0 18 2508.5 19 2680.5 20 2882.5 21 3252.5 22 3814.0 23 4516.5 24 5230.5 25 6089.0 26 7408.0 27 9002.5 28 10752.5 29 13023.0 30 15498.0 31 18102.0 32 21659.0 33 24196.5 34 26129.5 35 28336.5 36 31122.0 37 33994.5 38 35203.5 39 36169.5 40 36930.5 41 37952.5 42 38873.5 43 40279.0 44 42607.5 45 44593.5 46 47162.0 47 57765.5 48 86589.5 49 84971.0 50 56570.5 51 46636.0 52 42811.0 53 41756.0 54 40893.5 55 38257.5 56 34461.5 57 30928.5 58 28864.5 59 26316.5 60 22305.0 61 19225.5 62 16215.5 63 12160.0 64 8412.0 65 6035.0 66 4488.0 67 3567.5 68 2831.5 69 2209.0 70 1718.5 71 1278.0 72 1041.5 73 931.0 74 776.5 75 578.5 76 410.0 77 245.5 78 153.0 79 113.0 80 81.5 81 56.0 82 44.0 83 35.5 84 20.5 85 9.0 86 2.5 87 2.0 88 1.5 89 1.5 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.0048970816578413 2 0.013681425474235126 3 0.004488086663505702 4 0.0129575405285084 5 0.0 6 0.0 7 0.0 8 0.0 9 0.003764201717778976 10-11 4.70525214722372E-4 12-13 1.0858274185900893E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 3.619424728633631E-5 22-23 0.0012306044077354346 24-25 0.0 26-27 0.0 28-29 0.0 30-31 3.619424728633631E-5 32-33 0.0036556189759199675 34-35 4.343309674360357E-4 36-37 0.0030403167720522498 38-39 2.895539782906905E-4 40-41 0.0029317340301932408 42-43 0.009663864025451795 44-45 0.002425014568184533 46-47 0.002026877848034833 48-49 0.008505648112289033 50-51 4.70525214722372E-4 52-53 0.0018097123643168156 54-55 0.009881029509169813 56-57 0.003908978706924322 58-59 0.001773518117030479 60-61 0.005356748598377774 62-63 0.0033298707503429405 64-65 0.01567210907498362 66-67 0.0036194247286336313 68-69 3.9813672014969937E-4 70-71 0.0016649353751714703 72-73 0.0 74-75 3.619424728633631E-5 76-77 0.0011220216658764256 78-79 0.010279166229319512 80-81 0.014296727678102842 82-83 0.011944101604490982 84-85 0.008324676875857352 86-87 0.009084756068870414 88-89 0.025770304067871456 90-91 0.022187073586524157 92-93 0.0026421800519025504 94-95 0.007564597682844289 96-97 0.010025806498315158 98-99 0.009338115799874768 100-101 0.0109668569277599 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1381435.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.32148836837709 #Duplication Level Percentage of deduplicated Percentage of total 1 77.07901866372667 41.870470157875495 2 13.381208800660513 14.537743564398106 3 4.343996639297609 7.079170887416228 4 1.8250239670718964 3.965520727972218 5 0.9451887514946338 2.567202988511831 6 0.558207743069362 1.8193605253368228 7 0.35642472140534215 1.3553064950615636 8 0.24400198301214102 1.0603640705643973 9 0.1816708584222157 0.8881768280389924 >10 0.9659878887822653 9.546715258600495 >50 0.07237260779346981 2.6763539190226586 >100 0.038857865353690534 3.993146096670092 >500 0.0034837876277611845 1.315322263597656 >1k 0.003751771291435122 4.212510431393633 >5k 6.699591591848432E-4 2.266348808679111 >10k+ 1.3399183183696864E-4 0.8462869768607132 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 11627 0.8416610263964645 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7995 0.5787460141085176 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6686 0.48398947471288917 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5603 0.4055927350906847 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5568 0.4030591377806411 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 5285 0.3825731938165748 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4325 0.31308023902680904 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4052 0.2933181800084695 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3858 0.279274812061371 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3293 0.23837531262781092 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3109 0.22505582962643916 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2804 0.202977338781774 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2626 0.19009218674783832 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2593 0.1877033664269401 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2319 0.1678689189140278 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2059 0.14904791032513293 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2049 0.1483240253794062 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1997 0.1445598236616272 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1979 0.1432568307593191 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1804 0.1305888442091014 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1774 0.12841718937192123 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1753 0.1268970309858951 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1711 0.12385671421384285 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1561 0.11299844002794196 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1414 0.1023573313257591 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 7.238849457267262E-5 2 0.0 0.0 0.0 0.0 7.238849457267262E-5 3 0.0 0.0 0.0 0.0 7.238849457267262E-5 4 0.0 0.0 0.0 0.0 7.238849457267262E-5 5 0.0 0.0 0.0 0.0 1.4477698914534525E-4 6 0.0 0.0 0.0 0.0 1.4477698914534525E-4 7 0.0 0.0 0.0 0.0 1.4477698914534525E-4 8 0.0 0.0 0.0 0.0 2.1716548371801786E-4 9 0.0 0.0 0.0 0.0 2.895539782906905E-4 10-11 0.0 0.0 0.0 0.0 2.895539782906905E-4 12-13 0.0 0.0 0.0 0.0 4.343309674360357E-4 14-15 0.0 0.0 0.0 0.0 4.70525214722372E-4 16-17 0.0 0.0 0.0 0.0 5.067194620087083E-4 18-19 0.0 0.0 0.0 7.238849457267262E-5 5.79107956581381E-4 20-21 0.0 0.0 0.0 7.238849457267262E-5 6.514964511540536E-4 22-23 0.0 0.0 0.0 2.1716548371801786E-4 6.514964511540536E-4 24-25 0.0 0.0 0.0 6.876906984403899E-4 6.514964511540536E-4 26-27 0.0 0.0 0.0 7.962734402993988E-4 7.238849457267261E-4 28-29 0.0 0.0 0.0 0.0013753813968807797 7.238849457267261E-4 30-31 0.0 0.0 0.0 0.003583230481347295 7.962734402993988E-4 32-33 0.0 0.0 0.0 0.006913101231690235 8.686619348720714E-4 34-35 0.0 0.0 0.0 0.009700058272738132 9.41050429444744E-4 36-37 0.0 0.0 0.0 0.011690741873486628 9.41050429444744E-4 38-39 0.0 0.0 0.0 0.014115756441671162 9.41050429444744E-4 40-41 0.0 0.0 0.0 0.017011296224578067 9.41050429444744E-4 42-43 0.0 0.0 0.0 0.019544893534621605 9.41050429444744E-4 44-45 0.0 0.0 0.0 0.02265759880124653 9.41050429444744E-4 46-47 0.0 0.0 0.0 0.027145685464752232 9.41050429444744E-4 48-49 0.0 0.0 0.0 0.032538628310416345 0.0010134389240174166 50-51 0.0 0.0 0.0 0.04093569368084637 0.0010134389240174166 52-53 0.0 0.0 0.0 0.055630558079098905 0.0010134389240174166 54-55 0.0 0.0 0.0 0.07282282554010866 0.0010134389240174166 56-57 0.0 0.0 0.0 0.09627669778165458 0.0010858274185900894 58-59 0.0 0.0 0.0 0.13058884420910138 0.001158215913162762 60-61 0.0 0.0 0.0 0.17402194095270498 0.0011944101604490984 62-63 0.0 0.0 0.0 0.20865983560572882 0.0012306044077354346 64-65 0.0 0.0 0.0 0.24217570859287624 0.0012306044077354346 66-67 0.0 0.0 0.0 0.31308023902680904 0.0012667986550217707 68-69 0.0 0.0 0.0 0.40208189310391007 0.0013029929023081071 70-71 0.0 0.0 0.0 0.4693669988092093 0.0013753813968807797 72-73 0.0 0.0 0.0 0.5273139887146336 0.0018097123643168154 74-75 0.0 0.0 0.0 0.6523651130889256 0.001882100858889488 76-77 0.0 0.0 0.0 0.737747342437393 0.001882100858889488 78-79 0.0 0.0 0.0 0.8046343114225425 0.001954489353462161 80-81 0.0 0.0 0.0 0.8920434186190447 0.001990683600748497 82-83 0.0 0.0 0.0 1.051696243399074 0.0021716548371801788 84-85 0.0 0.0 0.0 1.2075486722140383 0.0021716548371801788 86-87 0.0 0.0 0.0 1.435138099150521 0.002244043331752851 88-89 0.0 0.0 0.0 1.7060520400887482 0.002316431826325524 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACCTG 1110 0.0 24.202444 1 ACCGGTC 100 0.0016554988 23.745846 8 TACCTGG 1035 0.0 22.945337 2 TACACCG 175 4.056372E-6 21.710487 5 TACATCG 110 0.0028805793 21.587132 5 GAGTACT 7710 0.0 21.311201 12-13 GTACATG 9105 0.0 20.917257 1 GTAAGGT 545 0.0 20.916044 4 AGTACTT 7935 0.0 20.67699 12-13 TACATGG 9025 0.0 20.577581 2 ACATGGG 8930 0.0 19.892298 3 GTACTTT 8375 0.0 19.59068 14-15 TATAGGG 460 0.0 18.585724 2 TAAGGTG 600 0.0 18.205149 5 TACTTTT 9080 0.0 18.017294 14-15 CATGGGA 5915 0.0 17.665777 4 ACTTTTT 9465 0.0 17.585455 16-17 CCGGTCA 190 1.8685184E-4 17.496939 9 GTATAGA 365 2.0099833E-9 17.086168 1 ATGGGAG 1905 0.0 16.952415 5 >>END_MODULE