##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936513_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1372236 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.31103250461291 31.0 31.0 34.0 28.0 34.0 2 31.306474250784852 31.0 31.0 34.0 28.0 34.0 3 31.363986223943986 31.0 31.0 34.0 28.0 34.0 4 34.982814909388765 37.0 35.0 37.0 32.0 37.0 5 34.97035422478349 37.0 35.0 37.0 32.0 37.0 6 35.1193468178943 37.0 35.0 37.0 32.0 37.0 7 34.9894260170991 37.0 35.0 37.0 32.0 37.0 8 34.93717552957363 37.0 35.0 37.0 32.0 37.0 9 36.37903538458399 39.0 37.0 39.0 32.0 39.0 10-11 36.29070874106203 39.0 35.0 39.0 32.0 39.0 12-13 36.18376649497608 39.0 35.0 39.0 32.0 39.0 14-15 37.16101676388027 39.0 36.0 41.0 31.0 41.0 16-17 37.055974701144706 39.0 36.0 41.0 31.0 41.0 18-19 37.056231945525404 39.0 36.0 41.0 31.0 41.0 20-21 36.96654693507531 39.0 36.0 41.0 30.5 41.0 22-23 36.83059947414293 39.0 36.0 41.0 30.5 41.0 24-25 36.96241717896922 39.0 36.0 41.0 31.0 41.0 26-27 36.916809863609465 39.0 36.0 41.0 30.5 41.0 28-29 36.83114566299091 39.0 36.0 41.0 30.0 41.0 30-31 36.65002812927222 39.0 36.0 41.0 30.0 41.0 32-33 36.45035438510577 39.0 35.0 41.0 30.0 41.0 34-35 36.20683905683862 39.0 35.0 40.0 30.0 41.0 36-37 35.985810385385605 38.0 35.0 40.0 29.0 41.0 38-39 35.74920931967971 38.0 35.0 40.0 28.5 41.0 40-41 35.582229295835404 38.0 35.0 40.0 27.0 41.0 42-43 35.33944270519065 38.0 34.5 40.0 27.0 41.0 44-45 35.03725634657596 38.0 34.0 40.0 25.5 41.0 46-47 34.69351627562605 38.0 34.0 40.0 24.0 41.0 48-49 34.50881517464926 38.0 33.5 40.0 24.0 41.0 50-51 33.55716910210781 37.0 32.0 39.5 22.5 40.5 52-53 33.690568531943484 37.5 33.0 39.5 23.0 40.5 54-55 34.11481151930134 38.0 33.0 40.0 23.0 41.0 56-57 34.01016479672593 38.0 33.0 40.0 22.5 41.0 58-59 33.73894140658021 37.5 33.0 40.0 21.5 41.0 60-61 33.37453215044643 37.0 32.5 40.0 20.5 41.0 62-63 33.03670323472056 36.5 32.0 40.0 19.5 41.0 64-65 32.675970459891744 36.0 32.0 39.0 18.0 41.0 66-67 32.25262782786635 35.5 31.0 39.0 15.5 40.5 68-69 31.844597066393828 35.0 31.0 38.5 13.0 40.0 70-71 31.376469863784365 35.0 30.5 38.0 9.5 40.0 72-73 30.958808834631945 35.0 30.0 37.0 7.5 39.5 74-75 30.538089658047156 35.0 30.0 37.0 7.0 39.0 76-77 30.090130997875 34.0 29.5 36.0 2.0 39.0 78-79 29.63693052798498 34.0 29.0 36.0 2.0 37.5 80-81 29.151866369924708 34.0 29.0 35.0 2.0 37.0 82-83 28.65897010426778 34.0 28.0 35.0 2.0 37.0 84-85 28.330435143809083 34.0 27.0 35.0 2.0 36.0 86-87 28.02450598876578 34.0 27.0 35.0 2.0 36.0 88-89 27.5827561731364 33.5 26.0 35.0 2.0 35.5 90-91 27.13503070900341 33.0 25.5 35.0 2.0 35.0 92-93 26.777246042225972 33.0 25.0 35.0 2.0 35.0 94-95 26.30100143124069 33.0 24.5 35.0 2.0 35.0 96-97 25.67914265476201 32.5 21.5 35.0 2.0 35.0 98-99 24.95902709154985 32.0 17.0 35.0 2.0 35.0 100-101 22.48327947962304 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6259877813492984 1101 2 0.5675918407787357 1101 3 0.6008468744550726 1101 4 0.6770855823376536 1101 5 0.6650272264393635 1101 6 0.4060087044359548 1101 7 0.3814557409575201 1101 8 0.6135257017957585 1101 9 0.8710685742417397 1101 10-11 0.8834072217757765 1101 12-13 0.9644242865358876 1101 14-15 1.141179412840465 1101 16-17 1.1935204845715006 1101 18-19 1.3111163285499288 1101 20-21 1.2319676061941607 1101 22-23 1.1935535031790039 1101 24-25 1.1429898902642535 1101 26-27 1.1767594746575796 1101 28-29 1.1893389395278078 1101 30-31 1.1986866272772403 1101 32-33 1.21946597825022 1101 34-35 1.1159271374237676 1101 36-37 1.2250148997165127 1101 38-39 1.1193381236511684 1101 40-41 1.0909203712421771 1101 42-43 1.1130622998906148 1101 44-45 1.1099463745827265 1101 46-47 1.0534974077532269 1101 48-49 1.3907235296725915 1101 50-51 1.2518197805191633 1101 52-53 1.3525589174133401 1101 54-55 1.4093167803279982 1101 56-57 1.1964078750055265 1101 58-59 1.2702284598144118 1101 60-61 1.3123239177884116 1101 62-63 1.4086188373050845 1101 64-65 1.390511758010362 1101 66-67 1.3851364593161932 1101 68-69 1.3877816585923064 1101 70-71 1.3373752084554162 1101 72-73 1.226693950920236 1101 74-75 1.2696638722554319 1101 76-77 1.222121098223898 1101 78-79 1.2989615354986803 1101 80-81 1.4208696062080044 1101 82-83 1.4580844230151122 1101 84-85 1.4031122514397296 1101 86-87 1.4526170974957822 1101 88-89 1.4007959939906343 1101 90-91 1.2789588781615322 1101 92-93 1.4782423402466023 1101 94-95 1.6060584064570342 1101 96-97 1.4217246621816528 1101 98-99 1.2612321676430263 1101 100-101 1.7841137433009173 1102 1 0.5511058457520583 1102 2 0.5068216215633434 1102 3 0.3877960902877824 1102 4 0.3871682823332634 1102 5 0.382705036921287 1102 6 0.43447046255219135 1102 7 0.4923939960205601 1102 8 0.31073608707010436 1102 9 0.5323121493416778 1102 10-11 0.483752354227569 1102 12-13 0.47655856145571107 1102 14-15 0.6291706156696151 1102 16-17 0.5471458854413314 1102 18-19 0.6053893813109852 1102 20-21 0.7334994231778609 1102 22-23 0.48619644347685664 1102 24-25 0.3436996495226978 1102 26-27 0.3201681861630945 1102 28-29 0.3090609115877285 1102 30-31 0.26980230842646336 1102 32-33 0.30638416785615874 1102 34-35 0.1128558155417636 1102 36-37 0.21299429610539278 1102 38-39 0.34268275089713285 1102 40-41 0.4405749642220087 1102 42-43 0.4972117419676607 1102 44-45 0.2666785166553822 1102 46-47 0.24772845155237633 1102 48-49 0.43715221877332766 1102 50-51 0.5260567549952881 1102 52-53 0.6602567164732207 1102 54-55 0.7072553209421457 1102 56-57 0.6107483068466664 1102 58-59 0.6072081527371367 1102 60-61 0.37539556915246663 1102 62-63 0.5893007553299512 1102 64-65 0.46256948326625746 1102 66-67 0.74505194716928 1102 68-69 0.6063954726765566 1102 70-71 0.5006818903514763 1102 72-73 0.486521576892212 1102 74-75 0.5536340211813204 1102 76-77 0.6536604932948435 1102 78-79 0.5179652366804213 1102 80-81 0.7028205792783382 1102 82-83 0.5029104564904863 1102 84-85 0.6629601954717081 1102 86-87 0.4577526244474157 1102 88-89 0.567132537943543 1102 90-91 0.859815147106751 1102 92-93 0.6759507925595649 1102 94-95 0.815545018502899 1102 96-97 0.635162313194261 1102 98-99 0.7743587326827814 1102 100-101 0.6853718156112478 1103 1 0.3197472227574032 1103 2 0.48288278466494816 1103 3 0.43523927210596725 1103 4 0.5869259694990419 1103 5 0.6423975502902692 1103 6 0.3945724010548659 1103 7 0.3340885147371324 1103 8 0.36938414856743407 1103 9 0.6375761867748366 1103 10-11 0.6502797606446791 1103 12-13 0.5521434545038346 1103 14-15 0.5700922400011663 1103 16-17 0.6250243947996168 1103 18-19 0.5797710658029658 1103 20-21 0.770564931199246 1103 22-23 0.7028241172736429 1103 24-25 0.799260311287405 1103 26-27 0.5796434535427792 1103 28-29 0.24366151319200213 1103 30-31 0.35645839131416324 1103 32-33 0.6567935929898496 1103 34-35 0.5028416109963132 1103 36-37 0.6756029191000366 1103 38-39 0.5804501305762741 1103 40-41 0.4008022369492821 1103 42-43 0.3518198636254226 1103 44-45 0.4226068254789155 1103 46-47 0.6351783178625325 1103 48-49 0.4462013497893693 1103 50-51 0.571841347241282 1103 52-53 0.7125210881309769 1103 54-55 0.8159744051667417 1103 56-57 0.7901141156701996 1103 58-59 0.43218308589221976 1103 60-61 0.37009393145193314 1103 62-63 0.495087018698932 1103 64-65 0.5228134672234788 1103 66-67 0.3363495741746334 1103 68-69 0.2534208737460837 1103 70-71 0.3205055868755373 1103 72-73 0.42930232555531234 1103 74-75 0.47847944230431594 1103 76-77 0.42454117511302414 1103 78-79 0.5181615936322927 1103 80-81 0.29144858729972967 1103 82-83 0.33406436691829455 1103 84-85 0.5970594601775936 1103 86-87 0.7221117487789641 1103 88-89 0.9214440352697437 1103 90-91 0.7183545856094256 1103 92-93 0.7239120225060915 1103 94-95 0.8230065826740223 1103 96-97 0.8082739442103026 1103 98-99 0.9638599024688794 1103 100-101 1.2481308396754187 1104 1 0.3288920870984917 1104 2 0.38292793089468447 1104 3 0.4436628978987791 1104 4 0.4991936315850154 1104 5 0.585271052756795 1104 6 0.4616696085953933 1104 7 0.49988529063565323 1104 8 0.5839773920487588 1104 9 0.5809011515387539 1104 10-11 0.6160677275227613 1104 12-13 0.5773042191268161 1104 14-15 0.6102200809102598 1104 16-17 0.5630611944824935 1104 18-19 0.7945019502286215 1104 20-21 0.9728200404311025 1104 22-23 0.6176298783730161 1104 24-25 0.673250026756044 1104 26-27 0.31009843944834614 1104 28-29 0.5164053028325952 1104 30-31 0.5172505424769582 1104 32-33 0.35917533189048356 1104 34-35 0.4737525738222459 1104 36-37 0.5334198236102594 1104 38-39 0.4654703913232794 1104 40-41 0.45171837507471935 1104 42-43 0.7155140364584014 1104 44-45 0.739580156169545 1104 46-47 0.6411794630351935 1104 48-49 0.5633448046871834 1104 50-51 0.5945823840629814 1104 52-53 0.8580295888356915 1104 54-55 0.8385833066818975 1104 56-57 0.7528271838520197 1104 58-59 0.9431668332495136 1104 60-61 1.1080283262722261 1104 62-63 1.2214210347313497 1104 64-65 1.0261498837139627 1104 66-67 1.066394390066101 1104 68-69 0.7206513177207121 1104 70-71 0.7650830842328311 1104 72-73 0.6437799506434025 1104 74-75 0.5523898765890216 1104 76-77 0.9261543269805408 1104 78-79 0.7988307488472337 1104 80-81 0.7529366510770359 1104 82-83 0.9309718501811552 1104 84-85 1.0089197050683616 1104 86-87 0.9321380657275213 1104 88-89 1.0268880098997677 1104 90-91 1.1409563297954683 1104 92-93 1.1306024735279365 1104 94-95 1.150057983308276 1104 96-97 1.0449172669728135 1104 98-99 1.1576941387268072 1104 100-101 1.4476386356585067 1105 1 0.3720330464195598 1105 2 0.3711051009125299 1105 3 0.4205975087275 1105 4 0.34920937536622176 1105 5 0.4698556418441058 1105 6 0.5463081763611228 1105 7 0.4719048429125081 1105 8 0.5426926995023109 1105 9 0.7048976729128142 1105 10-11 0.535089963739459 1105 12-13 0.575187055406488 1105 14-15 0.48813755350857946 1105 16-17 0.4725839330007844 1105 18-19 0.3756386512317249 1105 20-21 0.6315256017343955 1105 22-23 0.5158298393884095 1105 24-25 0.674010976181016 1105 26-27 0.5607866675982294 1105 28-29 0.531059764320311 1105 30-31 0.3730172646217085 1105 32-33 0.492374468231624 1105 34-35 0.49184517724389565 1105 36-37 0.6089997195513632 1105 38-39 0.3831056753860764 1105 40-41 0.29722390039222546 1105 42-43 0.6549896935738388 1105 44-45 0.4534301854079956 1105 46-47 0.5168695654447433 1105 48-49 0.6153150264495864 1105 50-51 0.8066601133599178 1105 52-53 0.6625825404198196 1105 54-55 0.7982544865658454 1105 56-57 0.8771985571595664 1105 58-59 0.9056306600379358 1105 60-61 0.7260422107878455 1105 62-63 0.7148378850754611 1105 64-65 0.6958610977844089 1105 66-67 0.7623887223048698 1105 68-69 0.7730856061767675 1105 70-71 1.007398619596362 1105 72-73 0.9857941050524133 1105 74-75 1.005349707455828 1105 76-77 0.6471445358480352 1105 78-79 1.1104271682615625 1105 80-81 0.836174772019266 1105 82-83 0.9517318999614908 1105 84-85 1.0347035204612816 1105 86-87 1.0575440303714885 1105 88-89 0.9901900056114599 1105 90-91 1.0741794566603566 1105 92-93 1.1614482894306555 1105 94-95 1.1472278512104523 1105 96-97 1.183360581863429 1105 98-99 1.386916096053664 1105 100-101 1.9488209186567218 1106 1 0.3082536004698113 1106 2 0.3476741943252257 1106 3 0.31773693927873836 1106 4 0.35302494859057987 1106 5 0.39144464967662174 1106 6 0.38743108312610275 1106 7 0.46641616229834426 1106 8 0.3727197949582717 1106 9 0.6166212696495137 1106 10-11 0.6044861644531139 1106 12-13 0.7060240532891839 1106 14-15 0.8856360358669235 1106 16-17 0.7329609003825865 1106 18-19 0.8666623560541211 1106 20-21 0.6161396765652256 1106 22-23 0.5874114440908826 1106 24-25 0.591032993089776 1106 26-27 0.28021618239708346 1106 28-29 0.6522260534273485 1106 30-31 0.7498447272729152 1106 32-33 0.49861752779721513 1106 34-35 0.5391519185341167 1106 36-37 0.4198949060362054 1106 38-39 0.44478041146449243 1106 40-41 0.39790794960823206 1106 42-43 0.25252074970437377 1106 44-45 0.5422544202945048 1106 46-47 0.5208272787659283 1106 48-49 0.5006753367888024 1106 50-51 0.3370603265700609 1106 52-53 0.5147176031243319 1106 54-55 0.5896113322401959 1106 56-57 0.3803656161921154 1106 58-59 0.6048592538874118 1106 60-61 0.7870129091991274 1106 62-63 0.8641242233392816 1106 64-65 0.7306832005694659 1106 66-67 0.8390429517130613 1106 68-69 0.7792005915926019 1106 70-71 0.9468299111464269 1106 72-73 0.9238913525686598 1106 74-75 0.7250783087084365 1106 76-77 0.8971408342830145 1106 78-79 0.9267347831211232 1106 80-81 0.921877847279486 1106 82-83 1.0509834435141094 1106 84-85 0.9482305196765495 1106 86-87 1.0221555881381121 1106 88-89 1.3115006557543438 1106 90-91 1.3555080223779434 1106 92-93 1.5161381723131377 1106 94-95 1.308360678169688 1106 96-97 1.6183966351067447 1106 98-99 1.9496872356915027 1106 100-101 1.855548142927141 1107 1 0.28817754816010677 1107 2 0.17859740927150014 1107 3 0.07511788254847573 1107 4 0.1433666170643022 1107 5 0.2949888913999388 1107 6 0.34758794987298813 1107 7 0.4657471967713249 1107 8 0.45517121346281897 1107 9 0.42135561805299204 1107 10-11 0.4385876743026458 1107 12-13 0.4523287982673381 1107 14-15 0.40167845281669656 1107 16-17 0.3569767628024607 1107 18-19 0.41913433957942914 1107 20-21 0.5789646102710293 1107 22-23 0.6496925598460095 1107 24-25 0.6420635826723355 1107 26-27 0.5515631983431248 1107 28-29 0.6087496720206147 1107 30-31 0.48548783416426033 1107 32-33 0.37657879866164023 1107 34-35 0.4109125025750302 1107 36-37 0.5005576921358994 1107 38-39 0.3041027720624214 1107 40-41 0.15879458404549496 1107 42-43 0.3886545258021812 1107 44-45 0.5371556841007745 1107 46-47 0.4875214410496014 1107 48-49 0.2604472648976852 1107 50-51 0.3015222415972616 1107 52-53 0.38076009093909846 1107 54-55 0.3712932986586921 1107 56-57 0.4750391199132409 1107 58-59 0.6034221118442034 1107 60-61 0.4485595801743898 1107 62-63 0.3135415005078599 1107 64-65 0.39359260534605056 1107 66-67 0.43088043844359447 1107 68-69 0.5301597492629604 1107 70-71 0.4891228471822693 1107 72-73 0.3919539178530371 1107 74-75 0.5795180713390629 1107 76-77 0.5038344579927418 1107 78-79 0.5835519245123741 1107 80-81 0.310509968679721 1107 82-83 0.6572922703158142 1107 84-85 0.6982400864505074 1107 86-87 0.5387677596257205 1107 88-89 0.481039480557655 1107 90-91 0.6992946507208231 1107 92-93 0.5508634358246098 1107 94-95 0.5570117630418601 1107 96-97 0.7446929329778165 1107 98-99 1.0611444041815226 1107 100-101 1.5674606898574837 1108 1 0.17614043013795921 1108 2 0.23789794779935747 1108 3 0.24878791032868364 1108 4 0.20054442010943774 1108 5 0.4134400303051464 1108 6 0.24048213102326343 1108 7 0.11320928532646235 1108 8 0.14870874034409098 1108 9 0.15682445937817135 1108 10-11 6.072301247499468E-4 1108 12-13 0.2764096982294433 1108 14-15 0.22932524296020063 1108 16-17 0.23067469573845756 1108 18-19 0.2330513765794393 1108 20-21 0.15871018744266507 1108 22-23 0.4534873833701951 1108 24-25 0.38941293995134885 1108 26-27 0.19750438649190016 1108 28-29 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-4.488545201303239 2316 40-41 -4.240365815388188 2316 42-43 -3.625593950774199 2316 44-45 -3.870162805330679 2316 46-47 -4.067469647927297 2316 48-49 -3.168236814651138 2316 50-51 -3.1575402332752844 2316 52-53 -3.7250396059538033 2316 54-55 -3.995533725710999 2316 56-57 -3.0563591320809245 2316 58-59 -3.412839360271061 2316 60-61 -2.8126809808078477 2316 62-63 -2.696744089894498 2316 64-65 -2.9718603606353184 2316 66-67 -3.202602718145119 2316 68-69 -4.063602432782474 2316 70-71 -3.87642808407308 2316 72-73 -3.283723263922674 2316 74-75 -2.8572190081863518 2316 76-77 -2.7787701663753204 2316 78-79 -3.7993009464045073 2316 80-81 -3.365121920060229 2316 82-83 -2.9936831584667374 2316 84-85 -2.7034582497174604 2316 86-87 -3.4008283791684235 2316 88-89 -4.101402496010067 2316 90-91 -3.799174291997229 2316 92-93 -4.489931339903613 2316 94-95 -4.843502847270095 2316 96-97 -5.655196735222706 2316 98-99 -6.829499632252396 2316 100-101 -7.271735292599356 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2906.0 3 1198.0 4 1241.0 5 1343.0 6 1485.0 7 1751.0 8 2242.0 9 2922.0 10 3763.0 11 4855.0 12 6415.0 13 7923.0 14 8704.0 15 10585.0 16 12736.0 17 16034.0 18 13575.0 19 10035.0 20 10348.0 21 10953.0 22 12229.0 23 13760.0 24 15474.0 25 17912.0 26 21080.0 27 24180.0 28 28780.0 29 34460.0 30 41098.0 31 50054.0 32 61906.0 33 78607.0 34 102040.0 35 137258.0 36 183671.0 37 226307.0 38 168062.0 39 24341.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.08573625949051 18.332075829314785 14.038774863041409 21.543413048153294 2 15.25936065870114 21.182043746246116 39.58992996635352 23.968665628699217 3 20.81253831301028 25.94336562620497 29.34676363032036 23.89733243046439 4 12.376070145865018 16.444192011619037 38.32285121418082 32.85688662833511 5 13.095929045580224 38.77220971335908 33.49029364070138 14.641567600359323 6 30.00051036031438 36.40166814914186 18.055308476355737 15.542513014188017 7 27.86303760902739 33.83281360845578 20.652046321011344 17.652102461505486 8 25.991691733873022 32.68992145203646 20.05007122829068 21.268315585799836 9 26.691245301286333 17.766605523850075 20.91346784752984 34.62868132733375 10-11 24.9527260663749 26.33858963819537 26.86778063144924 21.840903663980487 12-13 27.692424342947046 24.015122297259367 26.878530960173286 21.413922399620304 14-15 23.967804631957122 25.234528551682306 27.172469249566955 23.625197566793616 16-17 21.53300570476172 28.643014756409336 27.575080737561642 22.248898801267302 18-19 21.24335180312916 28.16013783262858 29.613672728439315 20.982837635802944 20-21 23.07809760086526 26.21028104816199 28.74945885000707 21.962162500965686 22-23 22.07710468215748 26.310996076396204 28.930728676565447 22.68117056488087 24-25 22.711849090045156 26.28819172164098 29.261359495567557 21.738599692746305 26-27 22.46818087569233 25.618080135326256 30.28070649807247 21.633032490908953 28-29 21.49464327424009 27.21947653815699 29.77396479329754 21.511915394305376 30-31 21.794559249505415 26.7374263769957 29.251768355053553 22.216246018445336 32-33 21.69482310870003 26.694551643747616 29.284728110541984 22.325897137010365 34-35 22.798787286918877 26.172410777404476 29.06517604017112 21.96362589550553 36-37 22.785858588803247 25.869936273423743 29.68748177991301 21.65672335786 38-39 22.481161963747304 26.298693016716822 29.644061509039854 21.576083510496026 40-41 22.054006823272292 26.725503392142123 29.639112806989864 21.58137697759572 42-43 21.597969698515534 27.185671289379904 28.471261687351063 22.745097324753498 44-45 22.005567681213815 26.738278901476093 28.398745085464633 22.85740833184546 46-47 22.863547457128117 27.142901908429213 27.70989952107084 22.283651113371832 48-49 22.71851452391016 26.999788663625367 27.785194793837725 22.496502018626753 50-51 22.31978044480914 27.44458104296003 28.38957637537175 21.846062136859082 52-53 22.454122573493883 27.919222497775454 27.491693928460293 22.13496100027037 54-55 21.657542877194874 27.246017277322267 28.065536781631394 23.030903063851465 56-57 22.61883026320153 27.40811925864945 27.13250163355283 22.840548844596192 58-59 23.291260925937937 26.91164284272261 27.243365724568335 22.55373050677111 60-61 22.561352683411226 27.01394772563703 28.400484662967923 22.02421492798382 62-63 22.675227889540256 27.085513600494405 28.097060392550116 22.14219811741522 64-65 22.28552523035396 28.019633649022474 27.684049973911197 22.01079114671237 66-67 22.146217391145914 27.425945697762977 27.35773083552274 23.07010607556837 68-69 22.00857216055837 27.719871618029014 27.622131057627936 22.64942516378468 70-71 22.754602300056845 27.4831285437346 26.964522570583178 22.797746585625372 72-73 22.429557488136755 27.02775578350248 27.705353554382178 22.837333173978582 74-75 21.939387711747834 28.571241174174972 27.049356844817428 22.440014269259766 76-77 22.480444736932245 27.369570239706388 27.22978827915871 22.920196744202656 78-79 22.233979754695117 27.38738640255945 27.77257919937034 22.606054643375092 80-81 22.673892121783247 27.808126778532223 27.049140601569793 22.46884049811474 82-83 22.42751178074764 27.54661648765372 27.46677851028383 22.559093221314807 84-85 22.257227738071116 27.742954476875465 27.234902762815906 22.76491502223751 86-87 22.34136362675715 28.035883073564015 27.112862097437723 22.509891202241107 88-89 22.512686267493493 27.64808297616957 26.541573716633643 23.2976570397033 90-91 22.212521589575786 27.29115501497606 27.252166245199277 23.244157150248874 92-93 22.57319668179173 27.965594890107948 27.253061303325676 22.208147124774648 94-95 22.16601388550797 27.784426249819617 27.209677583927444 22.839882280744973 96-97 21.43136951233955 27.594858293325075 27.659466698248142 23.314305496087236 98-99 21.92973952441485 27.993472074824776 26.683367931239577 23.3934204695208 100-101 22.059870924096284 27.467339189109396 27.10655942660147 23.36623046019285 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 724.0 1 515.0 2 566.5 3 779.5 4 649.0 5 635.0 6 923.5 7 1487.0 8 2091.5 9 2340.0 10 2034.0 11 1723.0 12 1742.0 13 1828.5 14 1905.0 15 1956.5 16 1967.5 17 1988.5 18 2120.5 19 2344.5 20 2602.0 21 2913.0 22 3436.0 23 4181.5 24 4843.5 25 5653.5 26 7019.5 27 8681.0 28 10417.0 29 12572.0 30 15077.5 31 17775.5 32 21283.0 33 23872.5 34 25888.0 35 28087.5 36 30949.5 37 33967.0 38 35203.5 39 35882.5 40 36436.5 41 37522.5 42 38628.5 43 40364.5 44 42778.0 45 45175.5 46 47906.5 47 57154.0 48 83338.5 49 82700.5 50 57486.0 51 48077.5 52 43705.0 53 42163.5 54 41231.0 55 38294.0 56 34709.0 57 31355.0 58 29227.5 59 26528.0 60 22503.5 61 19565.0 62 16555.5 63 12403.0 64 8442.0 65 6227.5 66 4742.0 67 3736.0 68 2972.0 69 2331.5 70 1833.0 71 1346.0 72 1077.0 73 990.5 74 752.5 75 530.5 76 381.0 77 234.0 78 167.5 79 117.5 80 78.5 81 60.0 82 44.5 83 25.5 84 19.5 85 17.5 86 10.0 87 5.5 88 5.0 89 3.5 90 2.0 91 1.0 92 1.5 93 2.5 94 1.0 95 0.5 96 0.5 97 0.0 98 1.0 99 2.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03432354201463888 2 0.02361109896548407 3 0.022809487580853438 4 0.050574390993968966 5 0.05567555435070935 6 0.04780518802888133 7 0.049335537035903446 8 0.04299551972109753 9 0.04255827714766265 10-11 0.051631060546436625 12-13 0.04510885882603284 14-15 0.03993482170705331 16-17 0.048278867483435794 18-19 0.05228692440658896 20-21 0.011514054433785443 22-23 0.020623274713678988 24-25 0.005829900979131869 26-27 0.01053025864355694 28-29 0.006012085384729741 30-31 0.009291404685491417 32-33 0.0037529987553161412 34-35 0.007724618797349727 36-37 0.009036346517654397 38-39 0.010566695524676513 40-41 0.0014939121259025417 42-43 0.0018947178182178576 44-45 0.0022590866294135994 46-47 5.829900979131869E-4 48-49 0.0011659801958263738 50-51 5.101163356740386E-4 52-53 0.003425066825239973 54-55 0.006595075482642927 56-57 0.016505907147167105 58-59 0.011441180671546295 60-61 0.010821753692513533 62-63 0.005829900979131869 64-65 0.0 66-67 0.007360249986153985 68-69 0.017234644769558587 70-71 0.006995881174958244 72-73 0.002587018559489767 74-75 0.004044493804272734 76-77 0.007214502461675688 78-79 0.004809668307783792 80-81 0.010384511119078644 82-83 0.005975648503610167 84-85 0.016760965315004126 86-87 0.004153804447631458 88-89 0.0060485222658493145 90-91 0.003352193063000825 92-93 0.0167974021961237 94-95 0.011514054433785443 96-97 0.008999909636534823 98-99 0.020696148475918136 100-101 0.035343774685986956 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1372236.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.41392737253882 #Duplication Level Percentage of deduplicated Percentage of total 1 78.29509287317312 44.1693368297337 2 12.8355270410539 14.482049805645458 3 4.068580208576533 6.88573765187956 4 1.6888332871341942 3.8109487361885774 5 0.8539646369958265 2.408774950509952 6 0.5065655284547086 1.7146410559005405 7 0.334764298901184 1.3219758191591189 8 0.23601303651766362 1.0651537840863863 9 0.17223661859775172 0.8744889683218712 >10 0.9003904525143845 9.236026646489224 >50 0.06357476135473923 2.4439921934043216 >100 0.03782770485146151 3.9667527524357094 >500 0.0025998242645176137 1.0706639698509839 >1k 0.0035097627570987782 4.443842140070543 >5k 3.899736396776421E-4 1.3681765538020414 >10k+ 1.2999121322588067E-4 0.7374381425220201 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10056 0.7328185530768759 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6495 0.4733150857432687 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6463 0.4709831253516159 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5699 0.41530757100090654 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4982 0.36305708347543714 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4843 0.35292763052419557 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4612 0.3360937914469523 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4179 0.30453945239740104 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3263 0.23778708618634112 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3166 0.23071833124914373 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2911 0.21213552187816087 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2691 0.19610329418554825 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2438 0.17766623233904372 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2187 0.15937491801701748 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2057 0.1499013289259282 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1914 0.13948038092572998 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1826 0.1330674898486849 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1797 0.1309541507437496 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1755 0.1278934527297054 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1704 0.12417689085550883 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1592 0.11601502948472421 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1580 0.11514054433785442 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1484 0.10814466316289618 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 1.4574752447829673E-4 0.0 6 0.0 0.0 0.0 1.4574752447829673E-4 0.0 7 0.0 0.0 0.0 1.4574752447829673E-4 0.0 8 0.0 0.0 0.0 1.4574752447829673E-4 0.0 9 0.0 0.0 0.0 1.4574752447829673E-4 7.287376223914836E-5 10-11 0.0 0.0 0.0 1.4574752447829673E-4 7.287376223914836E-5 12-13 0.0 0.0 0.0 1.4574752447829673E-4 1.821844055978709E-4 14-15 0.0 0.0 0.0 1.4574752447829673E-4 3.279319300761676E-4 16-17 0.0 0.0 0.0 1.4574752447829673E-4 3.643688111957418E-4 18-19 0.0 0.0 0.0 1.4574752447829673E-4 4.372425734348902E-4 20-21 0.0 0.0 0.0 1.821844055978709E-4 4.372425734348902E-4 22-23 0.0 0.0 0.0 4.0080569231531603E-4 4.736794545544644E-4 24-25 0.0 0.0 0.0 6.558638601523352E-4 5.101163356740386E-4 26-27 0.0 0.0 0.0 9.473589091089288E-4 5.829900979131869E-4 28-29 0.0 0.0 0.0 0.0014574752447829675 5.829900979131869E-4 30-31 0.0 0.0 0.0 0.0034979405874791215 6.194269790327611E-4 32-33 0.0 0.0 0.0 0.007979676965186746 6.558638601523353E-4 34-35 0.0 0.0 0.0 0.01158692819602459 6.558638601523353E-4 36-37 0.0 0.0 0.0 0.01384601482543819 6.923007412719095E-4 38-39 0.0 0.0 0.0 0.015886480168134345 8.016113846306321E-4 40-41 0.0 0.0 0.0 0.019347983874493893 8.016113846306321E-4 42-43 0.0 0.0 0.0 0.022700176937494716 8.016113846306321E-4 44-45 0.0 0.0 0.0 0.026088806881615113 9.109220279893546E-4 46-47 0.0 7.287376223914836E-5 0.0 0.030388358853724866 0.0010202326713480772 48-49 0.0 7.287376223914836E-5 0.0 0.03519802716150866 0.0010202326713480772 50-51 0.0 1.0931064335872255E-4 0.0 0.044380121203641355 0.0010202326713480772 52-53 0.0 1.4574752447829673E-4 0.0 0.0618333872599174 0.0010566695524676514 54-55 0.0 1.4574752447829673E-4 0.0 0.08085343920433512 0.0011295433147067997 56-57 0.0 1.4574752447829673E-4 0.0 0.105849139652363 0.0011659801958263738 58-59 0.0 1.4574752447829673E-4 0.0 0.1418487781985023 0.0012024170769459482 60-61 0.0 1.4574752447829673E-4 0.0 0.1923867323113517 0.0012388539580655224 62-63 0.0 1.4574752447829673E-4 0.0 0.2300624673889914 0.0013117277203046707 64-65 0.0 1.4574752447829673E-4 0.0 0.2678839499911094 0.0013481646014242448 66-67 0.0 1.4574752447829673E-4 0.0 0.33991966396450757 0.0014210383636633931 68-69 0.0 1.4574752447829673E-4 0.0 0.4301738184976928 0.0014574752447829673 70-71 0.0 1.4574752447829673E-4 0.0 0.5037034445969935 0.0014939121259025414 72-73 0.0 1.4574752447829673E-4 0.0 0.5668849964583351 0.0017489702937395608 74-75 0.0 1.4574752447829673E-4 0.0 0.6933574108243772 0.0017489702937395608 76-77 0.0 1.4574752447829673E-4 0.0 0.7778181012595502 0.0018218440559787093 78-79 0.0 1.4574752447829673E-4 0.0 0.8467566803377845 0.0018218440559787093 80-81 0.0 1.4574752447829673E-4 0.0 0.9424034932766667 0.0018218440559787093 82-83 0.0 1.4574752447829673E-4 0.0 1.1013047318391298 0.0018218440559787093 84-85 0.0 1.4574752447829673E-4 0.0 1.2615176981218974 0.0018218440559787093 86-87 0.0 1.4574752447829673E-4 0.0 1.491653039273128 0.0018218440559787093 88-89 0.0 1.4574752447829673E-4 0.0 1.7757514013624478 0.0018218440559787093 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9755 0.0 25.28587 1 TACATGG 9760 0.0 24.739073 2 ACATGGG 9630 0.0 23.93784 3 ATACCGT 140 1.7700768E-5 23.766829 6 GAGTACT 5610 0.0 22.747574 12-13 TACCTGG 925 0.0 22.608952 2 ATGGGCG 215 4.390131E-8 22.108675 5 CATGGGG 3335 0.0 21.808634 4 ATGGGAT 2090 0.0 21.606205 5 GTATAGG 465 0.0 21.46368 1 AGTACTT 5895 0.0 21.204382 12-13 GTACCTG 1055 0.0 21.17304 1 GTGTAGC 810 0.0 21.122986 1 GTACTTT 6180 0.0 20.45723 14-15 CATGGGA 5910 0.0 20.35007 4 TAGCGCG 125 0.0060045705 19.014847 4 TAGCCCT 830 0.0 18.900301 4 TAAGGTG 605 0.0 18.856325 5 GTGTAAG 665 0.0 18.581875 1 GTAGCCC 640 0.0 18.566479 3 >>END_MODULE