##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936513_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1372236 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.81175759854719 31.0 31.0 34.0 30.0 34.0 2 31.939297613530034 31.0 31.0 34.0 30.0 34.0 3 32.04520286597932 33.0 31.0 34.0 30.0 34.0 4 35.795487073652055 37.0 35.0 37.0 35.0 37.0 5 35.58081262989748 37.0 35.0 37.0 33.0 37.0 6 35.69143427223889 37.0 35.0 37.0 33.0 37.0 7 35.64524469551885 37.0 35.0 37.0 33.0 37.0 8 35.627908756219774 37.0 35.0 37.0 33.0 37.0 9 37.233656601342624 39.0 37.0 39.0 33.0 39.0 10-11 37.09648449683582 39.0 37.0 39.0 33.0 39.0 12-13 37.00322320650384 39.0 37.0 39.0 33.0 39.0 14-15 38.02271584479637 40.0 37.0 41.0 33.0 41.0 16-17 37.96975993925243 40.0 37.0 41.0 33.0 41.0 18-19 38.18543202481206 40.0 37.5 41.0 33.0 41.0 20-21 38.22652116691298 40.0 37.5 41.0 33.0 41.0 22-23 38.134538446739484 40.0 37.0 41.0 33.0 41.0 24-25 37.99328577591609 40.0 37.0 41.0 33.0 41.0 26-27 37.73452853590782 40.0 37.0 41.0 32.5 41.0 28-29 37.648058351478895 39.0 37.0 41.0 32.5 41.0 30-31 37.58595678877394 39.0 37.0 41.0 32.0 41.0 32-33 37.402708426247386 39.0 36.0 41.0 32.0 41.0 34-35 37.20808665564815 39.0 36.0 41.0 31.5 41.0 36-37 36.91351888450674 39.0 35.5 41.0 31.0 41.0 38-39 36.714834401662685 39.0 35.0 41.0 30.5 41.0 40-41 36.50045254606351 39.0 35.0 40.0 30.0 41.0 42-43 36.229705750322836 39.0 35.0 40.0 30.0 41.0 44-45 35.94164232683008 39.0 35.0 40.0 29.5 41.0 46-47 35.51888341364022 38.0 35.0 40.0 27.5 41.0 48-49 35.09584065714644 38.0 34.5 40.0 26.5 41.0 50-51 34.75933512894284 38.0 34.0 40.0 25.5 41.0 52-53 34.47269383692018 38.0 34.0 40.0 24.0 41.0 54-55 34.214652945994715 38.0 33.0 40.0 23.5 41.0 56-57 33.89730410803972 37.5 33.0 40.0 23.0 41.0 58-59 33.57408711038043 37.0 33.0 40.0 22.5 41.0 60-61 33.1636442273778 36.5 32.0 39.5 21.5 41.0 62-63 32.75656665471537 36.0 32.0 39.0 20.0 40.0 64-65 32.32256987865061 35.5 31.0 39.0 18.5 40.0 66-67 31.841055401549006 35.0 31.0 38.0 17.5 40.0 68-69 31.56012158258492 35.0 31.0 38.0 17.0 40.0 70-71 31.102656540128667 35.0 30.0 37.0 13.0 39.5 72-73 30.629946670980793 34.0 29.5 37.0 9.5 39.0 74-75 30.114625691207635 34.0 29.0 36.0 7.5 39.0 76-77 28.69024132875103 32.5 27.0 34.5 7.0 36.5 78-79 29.456590557309383 34.0 29.0 35.0 4.0 37.0 80-81 29.246536674449587 34.0 29.0 35.0 2.0 37.0 82-83 28.898438752517787 34.0 29.0 35.0 2.0 36.5 84-85 28.476697885786407 33.5 28.5 35.0 2.0 36.0 86-87 28.1054370385269 33.0 27.0 35.0 2.0 35.5 88-89 27.662159788840988 33.0 26.5 35.0 2.0 35.0 90-91 27.125452910432315 33.0 25.5 35.0 2.0 35.0 92-93 26.65485965970868 32.0 25.0 34.0 2.0 35.0 94-95 26.051803042625323 32.0 24.5 34.0 2.0 35.0 96-97 25.230190725210534 32.0 21.5 34.0 2.0 35.0 98-99 24.258141456717357 31.0 10.0 34.0 2.0 35.0 100-101 21.01317958426976 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5018042877050952 1101 2 0.5647725667208512 1101 3 0.5107551004679607 1101 4 0.3088611105573733 1101 5 0.3351716164991032 1101 6 0.3175176158893436 1101 7 0.3941019551487486 1101 8 0.42379112246788253 1101 9 0.42756055954521344 1101 10-11 0.5253990882532023 1101 12-13 0.5159372461701182 1101 14-15 0.6489439595686974 1101 16-17 0.6179035663434789 1101 18-19 0.6010698946340653 1101 20-21 0.48920982273412505 1101 22-23 0.4590396173249687 1101 24-25 0.445822635168895 1101 26-27 0.5270879061542502 1101 28-29 0.5725695480769559 1101 30-31 0.5082268331468924 1101 32-33 0.5648373758634122 1101 34-35 0.59286617300274 1101 36-37 0.49091138988470817 1101 38-39 0.5530013612662117 1101 40-41 0.7387866712324112 1101 42-43 0.7454206119280897 1101 44-45 0.7460907055977231 1101 46-47 0.4955764969364935 1101 48-49 0.18268929002116607 1101 50-51 0.15768704836878555 1101 52-53 0.17253201077660663 1101 54-55 0.30676179170028206 1101 56-57 0.327254670277739 1101 58-59 0.4291002860867863 1101 60-61 0.580196120318142 1101 62-63 0.5611413656705366 1101 64-65 0.36701193289367495 1101 66-67 0.23469648876267613 1101 68-69 0.3665440693587847 1101 70-71 0.3440052761962811 1101 72-73 0.5047466352999059 1101 74-75 0.39228394380186415 1101 76-77 0.35949409310625313 1101 78-79 0.2739633506328971 1101 80-81 0.46930081220757813 1101 82-83 0.5801890029240688 1101 84-85 0.6318647018500307 1101 86-87 0.6058026427036403 1101 88-89 0.39698294389803124 1101 90-91 0.576139469534823 1101 92-93 0.5398450855379018 1101 94-95 0.6990754832408257 1101 96-97 0.9159495531444648 1101 98-99 1.047523818362368 1101 100-101 1.652917551441103 1102 1 0.26874296011207477 1102 2 0.37439344270380204 1102 3 0.3070918091618964 1102 4 0.2063037806799386 1102 5 0.2098888110692414 1102 6 0.2025580323546592 1102 7 0.1961667093551398 1102 8 0.20424405046507133 1102 9 0.25971361494213596 1102 10-11 0.48008953986327185 1102 12-13 0.29836816476608874 1102 14-15 0.5679871985969385 1102 16-17 0.6065457707980855 1102 18-19 0.37786145330383647 1102 20-21 0.2020925710537682 1102 22-23 0.23296440755472503 1102 24-25 0.1823998108487288 1102 26-27 0.13505588049858375 1102 28-29 0.050393209281537565 1102 30-31 0.1749197668551119 1102 32-33 0.39187165297978055 1102 34-35 0.3661816960005879 1102 36-37 0.31245677054631216 1102 38-39 0.24388596566808474 1102 40-41 0.19425881901609188 1102 42-43 0.2662177937582513 1102 44-45 0.49872244350572714 1102 46-47 0.6733433224867227 1102 48-49 0.5625892373040386 1102 50-51 0.5574489975846149 1102 52-53 0.48939380403759714 1102 54-55 0.4891927102962512 1102 56-57 0.5900451636222002 1102 58-59 0.5515311497690831 1102 60-61 0.5952949100207263 1102 62-63 0.5926777404893215 1102 64-65 0.5769620163624651 1102 66-67 0.5237250109258333 1102 68-69 0.6828948908673738 1102 70-71 0.6464879234747585 1102 72-73 0.5306293726368132 1102 74-75 0.5186103836384426 1102 76-77 0.4397984796106691 1102 78-79 0.6528957189499032 1102 80-81 0.6550295715978187 1102 82-83 0.3558449467803264 1102 84-85 0.2226493633621338 1102 86-87 0.23725801985076345 1102 88-89 0.2566241379409959 1102 90-91 0.3846464216061669 1102 92-93 0.6090829935465116 1102 94-95 0.6425682126701631 1102 96-97 0.799206922559744 1102 98-99 0.539148109975951 1102 100-101 1.1945012069346674 1103 1 0.14251468471100281 1103 2 0.13944190527064748 1103 3 0.18733913536510727 1103 4 0.15535124057298333 1103 5 0.02431060251308992 1103 6 0.0894397168466412 1103 7 0.18180340721610122 1103 8 0.11114578843298517 1103 9 0.14993754542341264 1103 10-11 0.12642560269750192 1103 12-13 0.21591077840244566 1103 14-15 0.25443021330282534 1103 16-17 0.2975634847018256 1103 18-19 0.22140674073698818 1103 20-21 0.18320052559921862 1103 22-23 0.07474248242103698 1103 24-25 0.20785936967225638 1103 26-27 0.31313074680873854 1103 28-29 0.3907842520622893 1103 30-31 0.28447023476954314 1103 32-33 0.12547375859475807 1103 34-35 0.19420642862090887 1103 36-37 0.32562521974416825 1103 38-39 0.35564649374295243 1103 40-41 0.3740006305134216 1103 42-43 0.2763698659507625 1103 44-45 0.35057739002979815 1103 46-47 0.4761006754279009 1103 48-49 0.36570170388157663 1103 50-51 0.4807444521300681 1103 52-53 0.5352995527006996 1103 54-55 0.5727864602962498 1103 56-57 0.46981621977192844 1103 58-59 0.6637428810524995 1103 60-61 0.563180103870998 1103 62-63 0.5194365974411994 1103 64-65 0.24660188935711602 1103 66-67 0.671264282316212 1103 68-69 0.6392159135946471 1103 70-71 0.5149830772180728 1103 72-73 0.4033984234389507 1103 74-75 0.48380089165983264 1103 76-77 0.27126572559997797 1103 78-79 0.25128726306754956 1103 80-81 0.5302885956619896 1103 82-83 0.7238596526626786 1103 84-85 0.5865363954476983 1103 86-87 0.38100801985076416 1103 88-89 0.4338717983688021 1103 90-91 0.49158158203397306 1103 92-93 0.48462610851977317 1103 94-95 0.5906088209589306 1103 96-97 0.8305488343244498 1103 98-99 1.196317227623009 1103 100-101 1.3545446561325285 1104 1 0.12863497188507012 1104 2 0.23102818962582816 1104 3 0.19458455467447777 1104 4 0.14223681139750965 1104 5 0.0569149894052714 1104 6 0.12913536519054247 1104 7 0.22881604816043222 1104 8 0.0756344247966183 1104 9 0.1496402409836719 1104 10-11 0.24640721386733588 1104 12-13 0.17491855236298193 1104 14-15 0.29755312116047605 1104 16-17 0.33508440612536106 1104 18-19 0.20184245691034874 1104 20-21 0.16823069649421996 1104 22-23 0.30408532949643785 1104 24-25 0.19271336015851404 1104 26-27 0.22548099346131067 1104 28-29 0.17464392436673393 1104 30-31 0.2374355712741263 1104 32-33 0.34885124714302407 1104 34-35 0.3138879825321155 1104 36-37 0.28122775674204803 1104 38-39 0.2626810471256178 1104 40-41 0.35100082871637994 1104 42-43 0.28593315876260306 1104 44-45 0.39729823217214744 1104 46-47 0.34631077056532433 1104 48-49 0.13925206945592805 1104 50-51 0.09424868045987722 1104 52-53 0.18867560978315367 1104 54-55 0.09611660035967162 1104 56-57 0.4365236281581204 1104 58-59 0.1737218495162125 1104 60-61 0.1247193700795961 1104 62-63 0.21499409832209437 1104 64-65 0.5131600949596589 1104 66-67 0.15201932548041341 1104 68-69 0.3762749120090234 1104 70-71 0.4514287475845258 1104 72-73 0.24282649778708532 1104 74-75 0.4661057718571513 1104 76-77 0.3401852992433838 1104 78-79 0.47930049862075563 1104 80-81 0.5311750033434528 1104 82-83 0.48575183025252855 1104 84-85 0.36819381264290385 1104 86-87 0.31300238207768416 1104 88-89 0.5246872934357505 1104 90-91 0.7763662014842936 1104 92-93 0.7716845563703743 1104 94-95 0.7957736597538592 1104 96-97 0.9966704428515847 1104 98-99 1.4753980063404164 1104 100-101 1.7287188104665638 1105 1 0.15248305189346567 1105 2 0.26625827374260425 1105 3 0.1728041579311892 1105 4 0.08589806520225807 1105 5 0.055357104769697685 1105 6 0.11314418170802298 1105 7 0.18698857001429303 1105 8 0.13346808050261671 1105 9 0.07348082395339617 1105 10-11 0.09451121278776498 1105 12-13 -0.05606445934738957 1105 14-15 0.05825889238084159 1105 16-17 0.20104410204547207 1105 18-19 0.12079272155581577 1105 20-21 0.3244329965856849 1105 22-23 0.11873829157642035 1105 24-25 0.07562311080700823 1105 26-27 0.18606756176683348 1105 28-29 0.23005528687853882 1105 30-31 0.10697285404742729 1105 32-33 0.2605121410899258 1105 34-35 0.2861571861966681 1105 36-37 0.1780752519814328 1105 38-39 0.2679394700485602 1105 40-41 0.3326870626539744 1105 42-43 0.30191084757552034 1105 44-45 0.3210863358711862 1105 46-47 0.31331255486052356 1105 48-49 0.3784893207416715 1105 50-51 0.4445500614144038 1105 52-53 0.21255817616943062 1105 54-55 0.38405086632462115 1105 56-57 0.2327443709647099 1105 58-59 0.3204688844374175 1105 60-61 0.26070740480587773 1105 62-63 0.3108932181697597 1105 64-65 0.05359687243254996 1105 66-67 0.24640179440519105 1105 68-69 0.39065198306595406 1105 70-71 0.2878156247680401 1105 72-73 0.4366864231165799 1105 74-75 0.08117556928716851 1105 76-77 0.04612670238914163 1105 78-79 0.14955299888315565 1105 80-81 0.362494632090101 1105 82-83 0.3268738370598392 1105 84-85 0.3818196804251279 1105 86-87 0.3097314241060829 1105 88-89 0.5393994821212225 1105 90-91 0.6520405601126313 1105 92-93 0.6028512455281572 1105 94-95 0.6875306657364924 1105 96-97 0.6588178946081378 1105 98-99 0.9017028659208819 1105 100-101 0.8809086954619936 1106 1 0.12985168335084651 1106 2 0.23844027229158726 1106 3 0.24150224348176152 1106 4 0.166506306366621 1106 5 0.08224083120924064 1106 6 -0.003714226026772849 1106 7 0.06455264410482187 1106 8 0.10201514220030106 1106 9 0.04787931963737435 1106 10-11 0.11929985342502647 1106 12-13 0.26007658508619613 1106 14-15 0.258798580561006 1106 16-17 0.28671534384650244 1106 18-19 0.18970623819064514 1106 20-21 0.2308677972193891 1106 22-23 0.2213982362010043 1106 24-25 0.06793570056996145 1106 26-27 0.23219350381512527 1106 28-29 0.27745530760729764 1106 30-31 0.38803046520543205 1106 32-33 0.16730377259171547 1106 34-35 0.20651209225095357 1106 36-37 0.12561889343303534 1106 38-39 0.3017702937208071 1106 40-41 0.2926464935013371 1106 42-43 0.495357596861119 1106 44-45 0.32869527292977807 1106 46-47 0.36096280138475834 1106 48-49 0.26406136060012386 1106 50-51 0.5745201527690256 1106 52-53 0.46261518027076676 1106 54-55 0.2387925711174077 1106 56-57 0.26335557338109084 1106 58-59 0.41044652698468553 1106 60-61 0.25625294145877575 1106 62-63 0.29750243654729047 1106 64-65 0.46372953652293347 1106 66-67 0.42991872536359566 1106 68-69 0.33901441070035787 1106 70-71 0.3253946531812524 1106 72-73 0.1477082031251662 1106 74-75 0.07710310745030569 1106 76-77 -0.07648467770236067 1106 78-79 0.1103985843967088 1106 80-81 0.43700565535073466 1106 82-83 0.5626731530106248 1106 84-85 0.3939941277814718 1106 86-87 0.4533810191548717 1106 88-89 0.37403428571618136 1106 90-91 0.5300880585949912 1106 92-93 0.557425639581961 1106 94-95 0.7287905304072773 1106 96-97 0.799902305096893 1106 98-99 1.1659479687503556 1106 100-101 1.0066064404685804 1107 1 0.02832624352727109 1107 2 0.0701004831574572 1107 3 0.06645295687963682 1107 4 0.1053228893417355 1107 5 0.025808203266038277 1107 6 0.03835831988311611 1107 7 0.01690869250277416 1107 8 7.717450459523434E-4 1107 9 -0.1312092715240638 1107 10-11 0.01663136127616127 1107 12-13 -0.13287759219250006 1107 14-15 0.06193138013921384 1107 16-17 -0.010142360666314687 1107 18-19 0.10182939587801343 1107 20-21 0.03824328388472509 1107 22-23 0.014225139953587984 1107 24-25 0.1744401147503325 1107 26-27 0.30860226442893435 1107 28-29 0.10792286065328938 1107 30-31 0.24980121128623978 1107 32-33 0.2282462486428969 1107 34-35 0.37437850323671995 1107 36-37 0.246041192094971 1107 38-39 0.4218892902158302 1107 40-41 0.3268558598690916 1107 42-43 0.10353584215886968 1107 44-45 0.43548739897875066 1107 46-47 0.4713378614428336 1107 48-49 0.45904355222756266 1107 50-51 0.5181796493157762 1107 52-53 0.49991355165150964 1107 54-55 0.6691485826655637 1107 56-57 0.4843664766688036 1107 58-59 0.5162337874432978 1107 60-61 0.5673814381146585 1107 62-63 0.493468942917346 1107 64-65 0.5263337966428026 1107 66-67 0.7073650911941947 1107 68-69 0.933892005599219 1107 70-71 0.8147464794429702 1107 72-73 0.6681116516991494 1107 74-75 0.6640076820690162 1107 76-77 0.5820289716038012 1107 78-79 0.6263906872019689 1107 80-81 0.8855782218114676 1107 82-83 0.7054448529342316 1107 84-85 0.9240153731061547 1107 86-87 1.0979122968365083 1107 88-89 1.0063810366271504 1107 90-91 1.082404847787231 1107 92-93 1.1127095460004917 1107 94-95 1.2494299594055178 1107 96-97 1.3186205841547292 1107 98-99 1.8087047870255901 1107 100-101 1.747370251701252 1108 1 0.0072960296016262305 1108 2 0.0074901987886519805 1108 3 0.06015277792567986 1108 4 -0.09679929081450922 1108 5 -0.08074784236513466 1108 6 0.02774047752086517 1108 7 0.06740452963662591 1108 8 0.07910471047549095 1108 9 0.07037563025950533 1108 10-11 0.013113368003935477 1108 12-13 -0.06582747258906352 1108 14-15 0.14928631844938423 1108 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-0.352808502506722 2316 76-77 -0.6215357429839159 2316 78-79 -0.47661636324429324 2316 80-81 -1.0991633686967397 2316 82-83 -0.8580368990614566 2316 84-85 -1.218133002807722 2316 86-87 -1.0267880820982604 2316 88-89 -0.8418997618738011 2316 90-91 -0.9684220809072812 2316 92-93 -0.6820196169129638 2316 94-95 -0.9340633245024286 2316 96-97 -0.9605282833667026 2316 98-99 -1.257557397564394 2316 100-101 -1.4366187110475188 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 4.0 4 15.0 5 55.0 6 128.0 7 291.0 8 649.0 9 1275.0 10 2056.0 11 3399.0 12 4778.0 13 6521.0 14 8079.0 15 11005.0 16 13794.0 17 17451.0 18 15083.0 19 10163.0 20 8982.0 21 9552.0 22 10270.0 23 11364.0 24 13122.0 25 15735.0 26 19342.0 27 23609.0 28 29119.0 29 35838.0 30 43924.0 31 53896.0 32 66720.0 33 83818.0 34 108734.0 35 145933.0 36 200087.0 37 247490.0 38 142265.0 39 7689.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.742729958861 19.12559023861788 14.914875143162709 21.216804659358413 2 15.574382182965667 21.97541120998127 38.773475589759286 23.676731017293775 3 20.998549014663624 25.823093477416442 29.872297257411795 23.306060250508136 4 12.765006981347124 17.14933669914912 37.847646309505414 32.238010009998334 5 12.97985900281732 38.71118897324493 33.79020656947179 14.518745454465956 6 30.04608550157699 36.88450640999481 17.466507121244803 15.602900967183391 7 27.387708819765695 33.99145628011509 20.723694758044534 17.897140142074687 8 26.28265108917125 32.781897574469696 19.845565923062797 21.089885413296255 9 26.38177551704537 17.52955065514276 20.861742286222327 35.22693154158954 10-11 24.955428406524995 26.448071224185732 26.83299197911721 21.76350839017206 12-13 27.805795909057284 24.013783438812762 27.05129218827941 21.129128463850538 14-15 23.78562418060832 25.20170061805544 27.50193666115972 23.51073854017652 16-17 21.22494623593225 28.46380153315955 28.118953592008154 22.19229863890004 18-19 21.03308031563084 27.68441434272239 30.534871552706676 20.747633788940096 20-21 22.65794659227713 25.65513512252994 30.054560585788447 21.632357699404476 22-23 21.863057603344092 25.641008823709456 30.196062999011815 22.29987057393464 24-25 22.422306837435553 25.818579314616773 30.495800615788227 21.26331323215945 26-27 22.273118488012877 25.13524090480775 31.397352098593405 21.194288508585966 28-29 21.310010093538313 26.667711244638948 30.946897785616162 21.07538087620658 30-31 21.63688886394956 26.174612069939272 30.418723250443257 21.76977581566791 32-33 21.551679219838956 26.10995463951183 30.541939231010556 21.796426909638658 34-35 22.637377934260325 25.49924969517728 30.298394661065558 21.56497770949684 36-37 22.625554494314667 25.329835975725967 30.775167837817452 21.269441692141914 38-39 22.217067986474927 25.64607211403388 30.910085389855173 21.226774509636012 40-41 21.946320878845395 26.06325783344307 30.684163023211834 21.3062582644997 42-43 21.536925013135583 26.579203003584684 29.612083132018245 22.27178885126149 44-45 22.05801601705843 26.056268489594814 29.324829865667024 22.560885627679728 46-47 22.763613547523896 26.734577725697328 28.61264388924354 21.889164837535237 48-49 22.810536606410537 26.479467113823496 28.604482280791316 22.105513998974658 50-51 22.35614078428715 26.940768546311944 29.09329141607037 21.609799253330536 52-53 22.534208465677487 27.45061530237868 28.15520785243383 21.859968379510008 54-55 21.7919446262285 26.77714359839149 28.6056644721404 22.82524730323961 56-57 22.680058139513697 26.919403752979214 27.850415732502853 22.550122375004236 58-59 23.37556764540522 26.364325688452812 27.9326174888894 22.32748917725257 60-61 22.55168388005142 26.602477211577423 29.060631633566246 21.78520727480491 62-63 22.790721910188104 26.53290860740472 28.79110524210034 21.88526424030684 64-65 22.420093387448592 27.393408656449488 28.430042860276668 21.75645509582526 66-67 22.26440078165964 26.911180042974518 28.03852330021276 22.785895875153084 68-69 22.22588625050512 27.130311590989503 28.179976745363923 22.463825413141457 70-71 22.832851941230203 27.094875478735247 27.630680010829046 22.441592569205504 72-73 22.614865581299256 26.45046524855463 28.30807080624986 22.62659836389626 74-75 22.101189320131866 28.03317809266414 27.571719403674688 22.293913183529305 76-77 22.662984999684813 26.770754650636984 27.808719472935707 22.757540876742496 78-79 22.388877653827723 26.872978827679063 28.28761270740075 22.450530811092467 80-81 22.807663843793488 27.242092620994203 27.632492301503408 22.3177512337089 82-83 22.521743534694004 26.967813119106367 28.10428151505313 22.406161831146502 84-85 22.254838550994492 27.177361611884717 27.82370420844166 22.74409562867913 86-87 22.407937915710477 27.42645199420784 27.51714583671902 22.64846425336267 88-89 22.53107563681734 27.12653847723549 27.13517464741104 23.20721123853612 90-91 22.318271893044994 26.65657744274979 27.754609570356486 23.27054109384873 92-93 22.52433092240704 27.418608464355337 27.757802109712326 22.299258503525294 94-95 22.108274420153666 27.185277547210095 27.65149389637353 23.054954136262705 96-97 21.519117869968753 27.000110769087264 28.13356274194301 23.347208619000977 98-99 21.972498258276467 27.205430096399663 27.232394017216098 23.589677628107776 100-101 21.819273635839924 26.9798909116482 27.52238118541121 23.67845426710067 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 858.0 1 676.0 2 1029.5 3 1270.0 4 789.0 5 629.0 6 904.0 7 1664.0 8 2508.5 9 2892.5 10 2501.5 11 1889.5 12 1772.5 13 1830.0 14 1946.5 15 2070.0 16 2118.0 17 2271.0 18 2498.5 19 2744.0 20 2956.0 21 3220.5 22 3735.5 23 4381.0 24 5095.0 25 6038.0 26 7357.0 27 9043.5 28 10807.0 29 12880.0 30 15258.0 31 17915.0 32 21373.0 33 23890.5 34 25950.0 35 28350.5 36 31091.0 37 33831.0 38 34940.0 39 35552.0 40 36319.5 41 37625.5 42 38551.0 43 39930.5 44 42163.5 45 44196.0 46 46826.5 47 57167.5 48 86020.5 49 84558.5 50 56598.5 51 46809.5 52 42416.5 53 41467.0 54 40745.5 55 37961.5 56 34209.5 57 30719.5 58 28669.0 59 26122.5 60 22257.0 61 19067.5 62 15985.5 63 12111.5 64 8432.0 65 6085.0 66 4573.5 67 3601.0 68 2876.0 69 2244.5 70 1774.5 71 1386.0 72 1148.0 73 988.0 74 761.5 75 541.0 76 388.5 77 252.5 78 177.0 79 117.5 80 79.0 81 71.5 82 53.0 83 32.5 84 19.5 85 12.0 86 4.5 87 5.5 88 6.0 89 4.0 90 2.0 91 1.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.039716200420335857 2 0.004809668307783792 3 0.004736794545544644 4 5.829900979131869E-4 5 7.287376223914836E-4 6 2.9149504895659346E-4 7 0.0 8 0.0 9 0.0011659801958263738 10-11 4.00805692315316E-4 12-13 6.923007412719095E-4 14-15 0.0021133391049353027 16-17 0.0026963292028484897 18-19 0.0 20-21 0.0 22-23 0.001748970293739561 24-25 8.01611384630632E-4 26-27 0.00400805692315316 28-29 0.005174037118979534 30-31 0.0012388539580655221 32-33 4.736794545544644E-4 34-35 0.009254967804371843 36-37 0.011113248741470126 38-39 0.008562667063099935 40-41 0.0019311546993374317 42-43 6.558638601523352E-4 44-45 8.380482657502062E-4 46-47 0.0 48-49 7.651745035110579E-4 50-51 6.558638601523352E-4 52-53 0.0017854071748591351 54-55 0.0024777079161310443 56-57 0.0014210383636633931 58-59 7.651745035110579E-4 60-61 0.003497940587479122 62-63 0.004153804447631458 64-65 0.0075059975106322825 66-67 0.00200402846157658 68-69 0.0026598923217289157 70-71 3.643688111957418E-5 72-73 9.109220279893545E-4 74-75 0.004773231426664218 76-77 0.0022955235105331733 78-79 0.003170008657402954 80-81 0.003825872517555289 82-83 0.003388629944120399 84-85 0.0038987462797944374 86-87 0.0026963292028484897 88-89 0.007287376223914836 90-91 0.010566695524676513 92-93 8.01611384630632E-4 94-95 0.001384601482543819 96-97 8.744851468697805E-4 98-99 0.0023319603916527477 100-101 0.003315756181881251 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1372236.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.86129283300403 #Duplication Level Percentage of deduplicated Percentage of total 1 76.75962124952888 41.34372437871353 2 13.69037605250704 14.747627071160545 3 4.247450665046308 6.863195521913908 4 1.8394435223631938 3.9629922483110542 5 0.9868877491178094 2.657752502426927 6 0.5680920245919937 1.8358902535586097 7 0.36947549488772186 1.3930299477336667 8 0.24330491511858035 1.0483773824728835 9 0.1871821627020484 0.9073685950569029 >10 0.9854766008347106 9.639119078785951 >50 0.07491863066516069 2.7751943171772897 >100 0.039763904317751265 4.081130496308235 >500 0.0033928086096947506 1.2819650704541508 >1k 0.003799945642858121 4.291856489435252 >5k 6.785617219389502E-4 2.319203315963582 >10k+ 1.3571234438779003E-4 0.8515733305274751 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 11650 0.8489793300860785 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8201 0.5976377241232558 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7001 0.5101892094362778 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5647 0.4115181353644708 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5495 0.40044132350412026 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 5384 0.3923523358955748 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4300 0.313357177628338 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4155 0.30279048210366144 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3922 0.2858108955019399 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3172 0.23115557382257865 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3157 0.23006246738899142 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2880 0.20987643524874733 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2685 0.19566605161211334 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2655 0.1934798387449389 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2282 0.16629792542973656 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2118 0.15434662842251623 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2112 0.15390938584908134 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2061 0.1501928239748848 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1993 0.1452374081426227 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1888 0.1375856631075121 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1828 0.1332132373731632 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1801 0.13124564579270623 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1756 0.12796632649194453 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1513 0.11025800226783149 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1478 0.10770742058946128 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1449 0.10559408148452598 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 7.287376223914836E-5 7 0.0 0.0 0.0 0.0 7.287376223914836E-5 8 0.0 0.0 0.0 0.0 7.287376223914836E-5 9 0.0 0.0 0.0 0.0 7.287376223914836E-5 10-11 0.0 0.0 0.0 0.0 7.287376223914836E-5 12-13 0.0 0.0 0.0 0.0 7.287376223914836E-5 14-15 0.0 0.0 0.0 0.0 7.287376223914836E-5 16-17 0.0 0.0 0.0 0.0 1.4574752447829673E-4 18-19 0.0 0.0 0.0 0.0 2.186212867174451E-4 20-21 0.0 0.0 0.0 1.0931064335872255E-4 2.9149504895659346E-4 22-23 0.0 0.0 0.0 2.186212867174451E-4 3.279319300761676E-4 24-25 0.0 0.0 0.0 4.0080569231531603E-4 4.372425734348902E-4 26-27 0.0 0.0 0.0 6.923007412719095E-4 4.372425734348902E-4 28-29 0.0 0.0 0.0 0.0013117277203046707 4.372425734348902E-4 30-31 0.0 0.0 0.0 0.0033886299441203993 4.372425734348902E-4 32-33 0.0 0.0 0.0 0.007724618797349727 4.372425734348902E-4 34-35 3.643688111957418E-5 0.0 0.0 0.011404743790426719 4.372425734348902E-4 36-37 7.287376223914836E-5 0.0 0.0 0.013736704182079468 4.372425734348902E-4 38-39 7.287376223914836E-5 0.0 0.0 0.015886480168134345 4.372425734348902E-4 40-41 7.287376223914836E-5 0.0 0.0 0.019275110112254745 4.372425734348902E-4 42-43 7.287376223914836E-5 0.0 0.0 0.02255442941301642 4.736794545544644E-4 44-45 7.287376223914836E-5 0.0 0.0 0.026125243762734687 5.101163356740386E-4 46-47 7.287376223914836E-5 0.0 0.0 0.030898475189398908 5.101163356740386E-4 48-49 7.287376223914836E-5 0.0 0.0 0.0361453860706176 5.465532167936128E-4 50-51 7.287376223914836E-5 0.0 0.0 0.04536391699386986 6.558638601523353E-4 52-53 7.287376223914836E-5 0.0 0.0 0.06285361993126547 6.923007412719095E-4 54-55 7.287376223914836E-5 0.0 0.0 0.08172792435120489 7.287376223914836E-4 56-57 7.287376223914836E-5 0.0 0.0 0.10676006168035236 7.287376223914836E-4 58-59 7.287376223914836E-5 0.0 0.0 0.14235889453417633 7.287376223914836E-4 60-61 7.287376223914836E-5 0.0 0.0 0.19253247983583 8.016113846306321E-4 62-63 7.287376223914836E-5 0.0 0.0 0.23100982629810032 8.744851468697804E-4 64-65 7.287376223914836E-5 0.0 0.0 0.26945073587925106 9.109220279893545E-4 66-67 7.287376223914836E-5 0.0 0.0 0.34196012930720376 9.473589091089288E-4 68-69 7.287376223914836E-5 0.0 0.0 0.43290658458166087 9.473589091089288E-4 70-71 7.287376223914836E-5 0.0 0.0 0.5059625312264071 9.473589091089288E-4 72-73 7.287376223914836E-5 0.0 0.0 0.5693262674933466 0.0011659801958263738 74-75 7.287376223914836E-5 0.0 0.0 0.6971468464608128 0.0012024170769459482 76-77 7.287376223914836E-5 0.0 0.0 0.7818261581827033 0.0013117277203046707 78-79 7.287376223914836E-5 0.0 0.0 0.8513112904777312 0.0013117277203046707 80-81 7.287376223914836E-5 0.0 0.0 0.9473589091089287 0.0013481646014242448 82-83 7.287376223914836E-5 0.0 0.0 1.1078269335595334 0.001384601482543819 84-85 7.287376223914836E-5 0.0 0.0 1.2661451820240832 0.0014574752447829673 86-87 7.287376223914836E-5 0.0 0.0 1.495150979860607 0.0015303490070221158 88-89 7.287376223914836E-5 0.0 0.0 1.7782655461596986 0.0016032227692612641 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACCTG 1225 0.0 22.109894 1 TACCTGG 1170 0.0 21.922874 2 GTACATG 8870 0.0 21.803122 1 AGTACTT 8025 0.0 21.692947 12-13 TACATGG 8900 0.0 21.348118 2 GAGTACT 7845 0.0 21.282469 12-13 ACATGGG 8800 0.0 20.834278 3 GTAAGGT 570 0.0 20.832382 4 ATCACGC 115 0.003724625 20.651232 3 GTACTTT 8570 0.0 19.398886 14-15 CATGGGG 3095 0.0 18.722893 4 TACTTTT 9460 0.0 18.402315 14-15 CCGTACA 130 0.0075642588 18.269062 2 GTATGCG 130 0.0075658485 18.268396 9 CGATCGT 105 1.9551098E-5 18.09771 24-25 GTGTAGA 770 0.0 17.895967 1 TAAGGTG 650 0.0 17.53766 5 CATGGGT 960 0.0 17.316917 4 CATGGGA 5585 0.0 17.264208 4 ACTTTTT 9720 0.0 17.249413 16-17 >>END_MODULE