##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936512_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1409807 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.831713844519143 31.0 31.0 34.0 30.0 34.0 2 31.970839270907295 31.0 31.0 34.0 30.0 34.0 3 32.0585165203464 33.0 31.0 34.0 30.0 34.0 4 35.755282105990396 37.0 35.0 37.0 35.0 37.0 5 35.59195265734955 37.0 35.0 37.0 33.0 37.0 6 35.69692518195753 37.0 35.0 37.0 33.0 37.0 7 35.6442250605934 37.0 35.0 37.0 33.0 37.0 8 35.62742772592277 37.0 35.0 37.0 33.0 37.0 9 37.22826954327791 39.0 37.0 39.0 33.0 39.0 10-11 37.11211144504177 39.0 37.0 39.0 33.0 39.0 12-13 37.00803904364214 39.0 37.0 39.0 33.0 39.0 14-15 38.059756761031835 40.0 37.0 41.0 33.0 41.0 16-17 37.984195354399574 40.0 37.0 41.0 33.0 41.0 18-19 38.19131554886591 40.0 37.5 41.0 33.0 41.0 20-21 38.219251287587596 40.0 37.5 41.0 33.0 41.0 22-23 38.125838146639936 40.0 37.0 41.0 33.0 41.0 24-25 37.97932482956887 40.0 37.0 41.0 33.0 41.0 26-27 37.7443405373927 40.0 37.0 41.0 32.5 41.0 28-29 37.65369124993705 39.0 37.0 41.0 32.5 41.0 30-31 37.602073191578704 39.0 37.0 41.0 32.0 41.0 32-33 37.410451572449276 39.0 36.0 41.0 32.0 41.0 34-35 37.20840228485176 39.0 36.0 41.0 31.5 41.0 36-37 36.97574384295155 39.0 35.0 41.0 31.0 41.0 38-39 36.772455378644025 39.0 35.0 41.0 31.0 41.0 40-41 36.578385197406455 39.0 35.0 40.5 30.0 41.0 42-43 36.27697301829257 39.0 35.0 40.0 30.0 41.0 44-45 35.95933060340883 39.0 35.0 40.0 29.5 41.0 46-47 35.53256261318039 38.0 35.0 40.0 27.5 41.0 48-49 35.09005665314472 38.0 34.0 40.0 26.0 41.0 50-51 34.78474748671272 38.0 34.0 40.0 25.5 41.0 52-53 34.41917688023963 38.0 34.0 40.0 24.0 41.0 54-55 34.23692533800726 38.0 33.0 40.0 24.0 41.0 56-57 33.92630267830987 37.5 33.0 40.0 23.0 41.0 58-59 33.56783623574007 37.0 33.0 40.0 22.5 41.0 60-61 33.111165925548676 36.5 32.0 39.5 21.0 41.0 62-63 32.78040575766754 36.0 32.0 39.0 20.5 40.0 64-65 32.33743448571329 35.5 31.0 39.0 19.0 40.0 66-67 31.893227938292263 35.0 31.0 38.0 18.0 40.0 68-69 31.53933056084982 35.0 31.0 38.0 17.0 40.0 70-71 31.14755388503533 35.0 30.0 37.0 13.5 39.5 72-73 30.672647036083664 34.0 30.0 37.0 10.0 39.0 74-75 30.1831381884187 34.0 29.0 36.0 7.5 39.0 76-77 28.681160258106253 32.5 27.0 34.5 7.0 36.5 78-79 29.439632517075033 34.0 29.0 35.0 4.5 37.0 80-81 29.213347997279058 34.0 29.0 35.0 2.0 37.0 82-83 28.914428712582644 34.0 29.0 35.0 2.0 36.5 84-85 28.52944445587233 33.5 28.5 35.0 2.0 36.0 86-87 28.163638710830632 33.0 27.5 35.0 2.0 35.5 88-89 27.664895975122835 33.0 26.5 35.0 2.0 35.0 90-91 27.15077985851964 33.0 26.0 35.0 2.0 35.0 92-93 26.568561866978953 32.0 25.0 34.0 2.0 35.0 94-95 26.025487885930485 32.0 24.5 34.0 2.0 35.0 96-97 25.264096787716333 32.0 21.5 34.0 2.0 35.0 98-99 24.3740082862406 31.5 10.0 34.0 2.0 35.0 100-101 21.144884370697547 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.34990523183116196 1101 2 0.34582739087318615 1101 3 0.3395132439346682 1101 4 0.34291897673532645 1101 5 0.242411441916893 1101 6 0.2809986509303286 1101 7 0.327243583794818 1101 8 0.4006383662930517 1101 9 0.2563704487261589 1101 10-11 0.37310441555143825 1101 12-13 0.30365448311339094 1101 14-15 0.560391541563618 1101 16-17 0.6189260636368985 1101 18-19 0.5776087630950713 1101 20-21 0.49959801937163917 1101 22-23 0.34314078037122187 1101 24-25 0.5377217064324853 1101 26-27 0.4644079066043645 1101 28-29 0.2661557218874293 1101 30-31 0.4027639229181901 1101 32-33 0.48200699061454344 1101 34-35 0.3742016485938606 1101 36-37 0.28611037784872195 1101 38-39 0.3054596455896359 1101 40-41 0.2944041460669027 1101 42-43 0.31397018588837255 1101 44-45 0.30865223328630265 1101 46-47 0.3566235920671801 1101 48-49 0.4501435364740587 1101 50-51 0.2912418450224976 1101 52-53 0.33422470794129566 1101 54-55 0.1608534365103722 1101 56-57 0.21347479788313706 1101 58-59 0.05320071176907959 1101 60-61 0.23667925506268972 1101 62-63 0.2527991790723121 1101 64-65 0.131952985397362 1101 66-67 -0.05531914455783138 1101 68-69 0.07643049016066072 1101 70-71 0.04968565444396944 1101 72-73 0.3833866705940743 1101 74-75 0.2579352718768533 1101 76-77 0.1885849576294838 1101 78-79 0.1879176726072025 1101 80-81 0.3831938817988032 1101 82-83 0.2972445976706375 1101 84-85 -0.005815183734046769 1101 86-87 0.09475721365876666 1101 88-89 0.14053435945566406 1101 90-91 0.14423265996342494 1101 92-93 0.11758325167149408 1101 94-95 0.09695781614135868 1101 96-97 0.2406303506499583 1101 98-99 0.01917906479877729 1101 100-101 0.5596149036796874 1102 1 0.2629820674756296 1102 2 0.2605333846596558 1102 3 0.23132577708614122 1102 4 0.2902507566853174 1102 5 0.2543791239658404 1102 6 0.29024317620122275 1102 7 0.3179280196514469 1102 8 0.2956287050063935 1102 9 0.2853069494460172 1102 10-11 0.2970999258946989 1102 12-13 0.2563875853335915 1102 14-15 0.33468683594870186 1102 16-17 0.4175616436929772 1102 18-19 0.36368940642310577 1102 20-21 0.22877178992554548 1102 22-23 0.1998719503340851 1102 24-25 0.3137408774561976 1102 26-27 0.40414089516236373 1102 28-29 0.47811677364161653 1102 30-31 0.5509368031182404 1102 32-33 0.4329025540001581 1102 34-35 0.3233970149650034 1102 36-37 0.34135257304460254 1102 38-39 0.35290650851302985 1102 40-41 0.3624072907209879 1102 42-43 0.5423743686571854 1102 44-45 0.47886373968927387 1102 46-47 0.4343637981746227 1102 48-49 0.5544617527353282 1102 50-51 0.5526412160916792 1102 52-53 0.6961843389180373 1102 54-55 0.6745686748221118 1102 56-57 0.48988468270544416 1102 58-59 0.6741325523389108 1102 60-61 0.915055495874995 1102 62-63 0.927194875063833 1102 64-65 0.8774041295967976 1102 66-67 0.4540556186459206 1102 68-69 0.6636496498181543 1102 70-71 0.6322825514660195 1102 72-73 0.7669255430650885 1102 74-75 0.8783651044145522 1102 76-77 0.9565885758760544 1102 78-79 0.8899545938771887 1102 80-81 0.8947803218988284 1102 82-83 0.968367769603649 1102 84-85 0.9156371349747481 1102 86-87 0.9458052738239537 1102 88-89 0.8112336661162693 1102 90-91 1.0001895062163584 1102 92-93 0.851594769440684 1102 94-95 0.9154274493913412 1102 96-97 1.2277817865823657 1102 98-99 1.3865968491437037 1102 100-101 2.0743744134167486 1103 1 0.338530481433839 1103 2 0.3960994068477568 1103 3 0.3342070646554234 1103 4 0.2094290898885376 1103 5 0.2925051702676811 1103 6 0.3009176234502604 1103 7 0.24618044620431334 1103 8 0.2767828030399997 1103 9 0.26378891185288467 1103 10-11 0.37320374722754224 1103 12-13 0.4226809987279907 1103 14-15 0.46350665084544573 1103 16-17 0.4713803201265776 1103 18-19 0.4684933413854182 1103 20-21 0.3552894697159843 1103 22-23 0.3155189335151931 1103 24-25 0.48302221446731153 1103 26-27 0.33798669954621374 1103 28-29 0.318585018109772 1103 30-31 0.45458704121967486 1103 32-33 0.4043219790003789 1103 34-35 0.4405543592449348 1103 36-37 0.34868508885297445 1103 38-39 0.3735791016935224 1103 40-41 0.4501052748822545 1103 42-43 0.6063635868691222 1103 44-45 0.46175290976048444 1103 46-47 0.6074844665934407 1103 48-49 0.40611837924135585 1103 50-51 0.4590037070923998 1103 52-53 0.6136888602430801 1103 54-55 0.636394921493455 1103 56-57 0.3978309927837671 1103 58-59 0.6822877432971239 1103 60-61 0.6831513674823739 1103 62-63 0.6013336548048258 1103 64-65 0.6442590763817861 1103 66-67 0.43624869982593495 1103 68-69 0.5783687313277852 1103 70-71 0.7214250285015922 1103 72-73 0.6793695957336467 1103 74-75 0.5209315906619167 1103 76-77 0.8056248397219328 1103 78-79 0.4108451214301674 1103 80-81 0.669567104016501 1103 82-83 0.7216497961981467 1103 84-85 0.9093547158815305 1103 86-87 0.8874902101133877 1103 88-89 0.7171513433045043 1103 90-91 0.9210267428518861 1103 92-93 1.0202466989730645 1103 94-95 0.9713219236165358 1103 96-97 1.2845807968784086 1103 98-99 1.0898157723527397 1103 100-101 1.7531800788430374 1104 1 0.2321159456456492 1104 2 0.21677232644076483 1104 3 0.2654082608674173 1104 4 0.17594774042446915 1104 5 0.15296472511168702 1104 6 0.15624494551624224 1104 7 0.16961834739599624 1104 8 0.2040655994372642 1104 9 0.24545102224069382 1104 10-11 0.3106472971980949 1104 12-13 0.27320081728236545 1104 14-15 0.31668194926912463 1104 16-17 0.44165223683477706 1104 18-19 0.14546072245646258 1104 20-21 0.22882076203777046 1104 22-23 0.30706137235784325 1104 24-25 0.22956044945046727 1104 26-27 0.3196218750258808 1104 28-29 0.17401680734285918 1104 30-31 0.24433767935044415 1104 32-33 0.2102126685843686 1104 34-35 0.09510535180888269 1104 36-37 0.1858998179683482 1104 38-39 0.33261362368700986 1104 40-41 0.31371847953217724 1104 42-43 0.3496180286116868 1104 44-45 0.335556068444852 1104 46-47 0.40786935887898323 1104 48-49 0.5722022953252761 1104 50-51 0.45377757972804744 1104 52-53 0.6747319208541711 1104 54-55 0.5031209959949123 1104 56-57 0.32386974884412467 1104 58-59 0.44486407984754806 1104 60-61 0.46806807064961475 1104 62-63 0.4461233863116192 1104 64-65 0.5994547446444045 1104 66-67 0.42899716806036636 1104 68-69 0.4742660665339713 1104 70-71 0.6078875940138317 1104 72-73 0.7132026093722743 1104 74-75 0.6008806912373679 1104 76-77 0.3114694630611581 1104 78-79 0.25843709029643236 1104 80-81 0.8962447460289127 1104 82-83 0.7435826149992195 1104 84-85 0.7546594831085436 1104 86-87 0.8060372857878946 1104 88-89 0.7499600752493052 1104 90-91 0.9456598491264607 1104 92-93 0.9726685751530404 1104 94-95 0.9969649630824691 1104 96-97 1.2171795875765525 1104 98-99 0.6365125960474032 1104 100-101 1.164845914806925 1105 1 -0.09284359793793939 1105 2 0.08628252437347683 1105 3 0.022199836213545154 1105 4 0.10197105692728314 1105 5 0.05514281109797281 1105 6 0.061267858669801 1105 7 -0.0029456417767477205 1105 8 0.08630025151208542 1105 9 -0.09888094877647546 1105 10-11 0.017786093079074305 1105 12-13 0.13375520382702888 1105 14-15 0.2622072023343378 1105 16-17 0.19390005850581105 1105 18-19 0.22981417303646623 1105 20-21 0.0783015106151197 1105 22-23 0.21366609494588573 1105 24-25 0.22408534776271694 1105 26-27 0.06489588524082279 1105 28-29 0.1698261057581263 1105 30-31 0.1514322526020706 1105 32-33 0.17607547152152847 1105 34-35 0.14638659315266978 1105 36-37 0.038262083916443146 1105 38-39 -0.012394078825906263 1105 40-41 0.12469881261118587 1105 42-43 0.20004640496084392 1105 44-45 0.046253085118244996 1105 46-47 0.03044112345908445 1105 48-49 0.30848113115240494 1105 50-51 0.3061592841945284 1105 52-53 0.42615311953040447 1105 54-55 0.3151422617340387 1105 56-57 0.23698540316895134 1105 58-59 0.21398672231973848 1105 60-61 0.37390958840913413 1105 62-63 0.3464812980315912 1105 64-65 0.535943516470212 1105 66-67 0.37426486075683485 1105 68-69 0.5459047822310623 1105 70-71 0.49633771234581303 1105 72-73 0.5435886583476233 1105 74-75 0.3915033069815941 1105 76-77 0.41448923689399564 1105 78-79 0.35331576964349054 1105 80-81 0.21207547541072103 1105 82-83 0.6217446544885696 1105 84-85 0.665211439838405 1105 86-87 0.9393235617079441 1105 88-89 0.7686492387612631 1105 90-91 0.8380637621002158 1105 92-93 0.8277631678431874 1105 94-95 1.0419583296880681 1105 96-97 1.0365141098992616 1105 98-99 0.9515222910084553 1105 100-101 1.4765500897147135 1106 1 0.12103128562482013 1106 2 0.14242471111854016 1106 3 0.13093278438782363 1106 4 0.18256589920460442 1106 5 0.12256170328793559 1106 6 -0.049668633012295516 1106 7 0.012474393203312673 1106 8 0.02191528578386226 1106 9 0.06324023371556109 1106 10-11 -0.03340336730556004 1106 12-13 0.05392373197920364 1106 14-15 0.19851620972383444 1106 16-17 0.15541602966514034 1106 18-19 0.22175025310426832 1106 20-21 0.1658203444731896 1106 22-23 0.08936239323127637 1106 24-25 0.1646077974353588 1106 26-27 0.07209126562155888 1106 28-29 -0.003465182184434923 1106 30-31 0.04456230030258013 1106 32-33 -0.025113535753504834 1106 34-35 0.05315690903683645 1106 36-37 -0.1444722464805821 1106 38-39 0.11888798762095121 1106 40-41 0.15551813492140099 1106 42-43 0.190935324904963 1106 44-45 -0.0607520846237648 1106 46-47 -0.05977490488745474 1106 48-49 -0.3450827189055232 1106 50-51 -0.23961170086096217 1106 52-53 -0.28176408828425537 1106 54-55 -0.23675834223720216 1106 56-57 -0.3772831133931547 1106 58-59 -0.2584286809263361 1106 60-61 -0.12217363685358862 1106 62-63 -0.12398978809119399 1106 64-65 -0.11823775503162182 1106 66-67 -0.16282120574153325 1106 68-69 -0.2458682260780165 1106 70-71 0.041957024926091435 1106 72-73 0.198292913714468 1106 74-75 -0.17426149670509616 1106 76-77 -0.30950763808066384 1106 78-79 -0.301898353257954 1106 80-81 -0.05609481557626239 1106 82-83 -0.062165137591360775 1106 84-85 0.27995309820998315 1106 86-87 0.21639888712410027 1106 88-89 -0.018961600192618278 1106 90-91 0.21196574638637244 1106 92-93 0.17853223791443895 1106 94-95 0.3952054322906804 1106 96-97 0.5041165353233836 1106 98-99 0.5341768741166213 1106 100-101 0.8018582161637724 1107 1 0.08096716052462583 1107 2 0.03807580815403 1107 3 0.032457932940225476 1107 4 0.09865074792588757 1107 5 0.10643788777189656 1107 6 0.003731299215417039 1107 7 -0.0726174736237013 1107 8 0.0072131458762854095 1107 9 -0.017894620880227308 1107 10-11 0.03323937795276066 1107 12-13 -0.09563708638699353 1107 14-15 0.1235189655245108 1107 16-17 0.12438140456063707 1107 18-19 0.006841575575592174 1107 20-21 -0.055240913233419064 1107 22-23 0.017287431823824306 1107 24-25 -0.0029745964685830018 1107 26-27 -0.06344400159584751 1107 28-29 -0.08602760957531785 1107 30-31 -0.09665853527432944 1107 32-33 -0.1918762207153648 1107 34-35 -0.13883915708606054 1107 36-37 -0.26742382468113135 1107 38-39 -0.20764377258492317 1107 40-41 -0.1603537799994328 1107 42-43 -0.13906420785640705 1107 44-45 -0.11876429724365067 1107 46-47 -0.25537483248814397 1107 48-49 -0.032083867949658895 1107 50-51 -0.03547015205108295 1107 52-53 0.09376648840162716 1107 54-55 0.05106723349832265 1107 56-57 -0.10883977560366986 1107 58-59 -0.04854848907724829 1107 60-61 0.12004672649476333 1107 62-63 0.2231335869503681 1107 64-65 0.07242305194846921 1107 66-67 0.15606167658554781 1107 68-69 0.1337588539844603 1107 70-71 0.10317413166653111 1107 72-73 0.24072838828602627 1107 74-75 0.1500173113918315 1107 76-77 -0.0036239696132405186 1107 78-79 0.16289657661315715 1107 80-81 0.6005389977124587 1107 82-83 0.36670367767745304 1107 84-85 0.38913066295315346 1107 86-87 0.2338343730364052 1107 88-89 0.12784636608999023 1107 90-91 0.42341164128671593 1107 92-93 0.4067901046479996 1107 94-95 0.2889435727081384 1107 96-97 0.4212889613488464 1107 98-99 0.4027102120467383 1107 100-101 0.9149018403750802 1108 1 0.01453298659927782 1108 2 0.09003023384500963 1108 3 0.035587539583282535 1108 4 0.07554028879750518 1108 5 0.10853354653416858 1108 6 0.08699522504701207 1108 7 0.12055927638570552 1108 8 0.04203914350836868 1108 9 0.013087992316691555 1108 10-11 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-0.7681090404285307 2316 82-83 -0.95468725537431 2316 84-85 -0.9354271832738554 2316 86-87 -1.0749989797758097 2316 88-89 -1.280312396181703 2316 90-91 -1.1657027212300122 2316 92-93 -1.2756183513351047 2316 94-95 -1.370579382815535 2316 96-97 -1.7106778446253905 2316 98-99 -2.4049657426870326 2316 100-101 -3.217606008269996 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 5.0 4 27.0 5 48.0 6 124.0 7 296.0 8 647.0 9 1174.0 10 2080.0 11 3321.0 12 4916.0 13 6676.0 14 8294.0 15 10908.0 16 13944.0 17 17676.0 18 15249.0 19 10334.0 20 9362.0 21 9768.0 22 10391.0 23 11585.0 24 13731.0 25 16509.0 26 19869.0 27 24479.0 28 30048.0 29 36962.0 30 45335.0 31 55540.0 32 68359.0 33 86269.0 34 112476.0 35 150289.0 36 204706.0 37 253152.0 38 147218.0 39 8038.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.788629272217236 19.107862710125616 14.931174858852058 21.172333158805092 2 15.577320487199623 22.017980898221985 38.71249220527424 23.692206409304152 3 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21.630726759052834 22-23 21.85196271546389 25.705929960625816 30.073265347668155 22.368841976242138 24-25 22.438957956656477 25.829457507304195 30.383875239660462 21.347709296378866 26-27 22.309520457117564 25.14969422761751 31.31617782925903 21.2246074860059 28-29 21.340748241294254 26.65965607926102 30.84263595722005 21.156959722224684 30-31 21.708004945413894 26.045066254501553 30.38113450859028 21.86579429149427 32-33 21.569749913989707 26.125245173669853 30.43086723202917 21.874137680311268 34-35 22.684882573227924 25.518666386247347 30.260708394527285 21.535742645997445 36-37 22.652866595569083 25.211776568228316 30.85144376913171 21.283913067070884 38-39 22.22754609097006 25.68748543669693 30.84253119339818 21.242437278934823 40-41 21.94406318604346 26.06856976674079 30.581534325669153 21.4058327215466 42-43 21.54383351510476 26.545757632183296 29.596822103405774 22.313586749306165 44-45 22.140231610277333 25.97795261962488 29.318681895498738 22.56313387459905 46-47 22.819530779245788 26.725885237360757 28.54950558782754 21.905078395565912 48-49 22.878438699417437 26.448202899846006 28.52133531092021 22.15202308981635 50-51 22.309354020820603 26.91910337986339 29.138203816058315 21.63333878325769 52-53 22.63297219617451 27.456437268673845 28.060917601428848 21.849672933722797 54-55 21.826954246344666 26.752336697193694 28.565338501009723 22.855370555451916 56-57 22.71052552235656 26.886824075666986 27.800311791131865 22.602338610844587 58-59 23.46126352721229 26.308696261425297 27.86319267929709 22.36684753206532 60-61 22.639339546106445 26.58236110209501 28.987336428723204 21.79096292307534 62-63 22.822771587126205 26.52220564944497 28.758040802138186 21.896981961290642 64-65 22.430703503719958 27.399030476908028 28.393100253341647 21.777165766030368 66-67 22.36115317856137 26.824885992283864 28.0087999982976 22.805160830857165 68-69 22.23424127632424 27.142637759935194 28.16508884071022 22.458032123030346 70-71 22.892164912110218 27.063307669036618 27.583273011773613 22.461254407079558 72-73 22.603164830363305 26.464792698575053 28.215883450713463 22.716159020348176 74-75 22.16763549051806 28.087084200782385 27.506109087555902 22.23917122114365 76-77 22.67551724529287 26.751590323569136 27.7894502389032 22.783442192234794 78-79 22.417240975692806 26.850918480631925 28.28349288660781 22.448347657067462 80-81 22.871767403831317 27.154464046595855 27.65770351544002 22.31606503413281 82-83 22.51803131683151 27.00669676349702 28.024424617159312 22.450847302512162 84-85 22.277723163747602 27.178263150855464 27.801619925847117 22.742393759549817 86-87 22.46304227074645 27.392453876881582 27.49006669026589 22.65443716210608 88-89 22.596737402290426 27.06883870411495 27.102900232718298 23.231523660876324 90-91 22.333317011518233 26.59657511547684 27.74900170811343 23.321106164891493 92-93 22.588826454537294 27.33871319643974 27.71277533602267 22.35968501300029 94-95 22.23390592959975 27.158020959719593 27.61290505686938 22.995168053811277 96-97 21.539225259954637 26.993801682218056 28.15142659244393 23.31554646538338 98-99 22.082936050152956 27.26955298589581 27.10149643429844 23.54601452965279 100-101 21.81408853758195 27.008625504893324 27.44841766379623 23.7288682937285 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 857.0 1 668.5 2 1112.5 3 1423.5 4 829.0 5 612.0 6 930.0 7 1703.5 8 2555.0 9 2902.5 10 2476.5 11 1880.5 12 1764.5 13 1824.5 14 1946.5 15 2082.5 16 2211.5 17 2377.0 18 2523.0 19 2675.0 20 2967.5 21 3413.5 22 3886.5 23 4454.5 24 5139.5 25 6090.5 26 7446.5 27 9168.5 28 10882.5 29 13033.5 30 15619.5 31 18178.5 32 21679.0 33 24407.0 34 26528.0 35 28721.0 36 31407.0 37 34490.5 38 35948.0 39 36699.5 40 37531.0 41 38778.0 42 39563.0 43 41014.0 44 43269.5 45 45415.0 46 48028.5 47 58768.5 48 88537.0 49 87143.0 50 58250.5 51 48071.0 52 44173.5 53 42841.5 54 41818.5 55 39075.5 56 35176.5 57 31739.5 58 29831.5 59 27133.5 60 22972.5 61 19873.5 62 16666.0 63 12486.0 64 8675.0 65 6276.0 66 4665.5 67 3694.0 68 2946.5 69 2325.0 70 1878.0 71 1364.5 72 1098.5 73 1017.0 74 770.0 75 563.0 76 413.5 77 258.5 78 173.0 79 137.5 80 101.5 81 72.0 82 58.0 83 35.5 84 18.0 85 12.0 86 4.0 87 0.5 88 1.5 89 2.0 90 1.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020782986607386686 2 0.01418633898115132 3 0.009292052032654116 4 0.0 5 0.0021988825420784547 6 0.0 7 0.0 8 0.0 9 7.093169490575661E-5 10-11 0.0 12-13 0.0011349071184921058 14-15 1.4186338981151322E-4 16-17 0.0 18-19 0.0012058388133978624 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.001170372965944984 28-29 0.0024471434742486028 30-31 0.0011349071184921058 32-33 0.006525715931329608 34-35 9.575778812277141E-4 36-37 5.319877117931745E-4 38-39 9.575778812277141E-4 40-41 1.4186338981151322E-4 42-43 0.0049297527959500835 44-45 0.001773292372643915 46-47 1.0639754235863491E-4 48-49 5.674535592460529E-4 50-51 1.0639754235863491E-4 52-53 7.093169490575661E-5 54-55 0.0012767705083036188 56-57 0.01486019008275601 58-59 0.026918578216734632 60-61 0.02067658906502805 62-63 0.014150873133698443 64-65 0.017591060336627638 66-67 0.002411677626795725 68-69 6.029194066989312E-4 70-71 1.0639754235863491E-4 72-73 0.0 74-75 0.001560497287926645 76-77 0.0021988825420784547 78-79 0.010072300676617438 80-81 0.016988140929928706 82-83 0.01741373109936325 84-85 0.029897709402776404 86-87 0.011065344405298029 88-89 0.04202702923166079 90-91 0.046105601688741796 92-93 0.029472119233341868 94-95 0.030997150673815636 96-97 0.05071616185761597 98-99 0.053730758891110626 100-101 0.06000821389027009 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1409807.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.28173477225499 #Duplication Level Percentage of deduplicated Percentage of total 1 76.27096154726918 40.63849143986455 2 13.859765498075435 14.769446985482118 3 4.434331297910004 7.088065923226501 4 1.902610582383938 4.054975697018664 5 0.9810674835500903 2.613648872609977 6 0.5880167396636155 1.8798331178641725 7 0.3724483836687455 1.3891287196495217 8 0.2597586091668107 1.107231145474868 9 0.18967491608901266 0.9095587714804043 >10 1.0176729343087214 9.80816445608141 >50 0.07524277958486354 2.769891995056925 >100 0.04041901233454284 4.148297413497283 >500 0.0034797602645171713 1.3244525130413731 >1k 0.003747434131018492 4.3281279721387556 >5k 6.691846662533022E-4 2.2884297418839554 >10k+ 1.3383693325066042E-4 0.8822552356295628 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 12372 0.8775669293740207 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8100 0.5745467287366285 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7060 0.5007777660346416 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5901 0.4185679316388697 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5604 0.39750121825186 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 5426 0.38487537655863535 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4457 0.3161425641949572 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4290 0.3042969711456958 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4012 0.2845779599618955 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3513 0.24918304420392295 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3355 0.23797583640881342 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2973 0.2108799289548144 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2887 0.20477980319291933 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2803 0.19882154082083575 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2368 0.16796625353683164 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2161 0.15328339269134 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2134 0.1513682369288846 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2098 0.14881469591227736 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2077 0.14732513031925645 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1918 0.13604699082924115 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1905 0.13512487879546634 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1804 0.12796077760998492 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1718 0.12186065184808985 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1513 0.10731965439240974 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1490 0.10568822540957734 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1489 0.10561729371467159 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1426 0.10114859693560892 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 7.09316949057566E-5 2 0.0 0.0 0.0 0.0 7.09316949057566E-5 3 0.0 0.0 0.0 0.0 7.09316949057566E-5 4 0.0 0.0 0.0 7.09316949057566E-5 7.09316949057566E-5 5 0.0 0.0 0.0 7.09316949057566E-5 7.09316949057566E-5 6 0.0 0.0 0.0 7.09316949057566E-5 2.1279508471726982E-4 7 0.0 0.0 0.0 7.09316949057566E-5 2.1279508471726982E-4 8 0.0 0.0 0.0 7.09316949057566E-5 2.837267796230264E-4 9 0.0 0.0 0.0 7.09316949057566E-5 2.837267796230264E-4 10-11 0.0 0.0 0.0 7.09316949057566E-5 2.837267796230264E-4 12-13 0.0 0.0 0.0 7.09316949057566E-5 3.54658474528783E-4 14-15 0.0 0.0 0.0 1.773292372643915E-4 4.610560168874179E-4 16-17 0.0 0.0 0.0 2.837267796230264E-4 5.674535592460528E-4 18-19 0.0 0.0 0.0 3.191926270759047E-4 5.674535592460528E-4 20-21 0.0 0.0 0.0 4.965218643402963E-4 6.029194066989311E-4 22-23 0.0 0.0 0.0 7.447827965104443E-4 7.09316949057566E-4 24-25 0.0 0.0 0.0 0.001170372965944984 7.802486439633226E-4 26-27 0.0 0.0 0.0 0.0015250314404737669 7.802486439633226E-4 28-29 0.0 0.0 0.0 0.0024471434742486028 7.802486439633226E-4 30-31 0.0 0.0 0.0 0.00500068449085584 8.15714491416201E-4 32-33 0.0 0.0 0.0 0.008511803388690793 9.221120337748359E-4 34-35 3.54658474528783E-5 0.0 0.0 0.01110081025275091 9.221120337748359E-4 36-37 7.09316949057566E-5 0.0 0.0 0.013406090337187999 9.221120337748359E-4 38-39 7.09316949057566E-5 0.0 0.0 0.015392177794549183 9.221120337748359E-4 40-41 7.09316949057566E-5 0.0 0.0 0.018406774828043836 9.221120337748359E-4 42-43 7.09316949057566E-5 0.0 0.0 0.021421371861538495 9.221120337748359E-4 44-45 7.09316949057566E-5 0.0 0.0 0.024967956606826325 9.221120337748359E-4 46-47 7.09316949057566E-5 0.0 0.0 0.029578516775700503 9.221120337748359E-4 48-49 7.09316949057566E-5 0.0 0.0 0.036246096096841623 9.221120337748359E-4 50-51 7.09316949057566E-5 0.0 0.0 0.044296843468645 0.0010639754235863491 52-53 7.09316949057566E-5 0.0 0.0 0.05951169202592979 0.0010639754235863491 54-55 7.09316949057566E-5 0.0 0.0 0.07876964719284271 0.0010639754235863491 56-57 7.09316949057566E-5 0.0 0.0 0.10320561608787586 0.0010639754235863491 58-59 7.09316949057566E-5 0.0 0.0 0.13753655642226204 0.0010639754235863491 60-61 7.09316949057566E-5 0.0 0.0 0.18633756251742262 0.0010639754235863491 62-63 7.09316949057566E-5 0.0 0.0 0.22272552200407575 0.0011349071184921056 64-65 7.09316949057566E-5 0.0 0.0 0.25833323284676557 0.0011349071184921056 66-67 7.09316949057566E-5 0.0 0.0 0.33096728843026035 0.001170372965944984 68-69 7.09316949057566E-5 0.0 0.0 0.42392327460425433 0.0012058388133978622 70-71 7.09316949057566E-5 0.0 0.0 0.49492590120491664 0.0012767705083036188 72-73 7.09316949057566E-5 0.0 0.0 0.5582324389083044 0.0013477022032093754 74-75 7.09316949057566E-5 0.0 0.0 0.6836042096542293 0.0013477022032093754 76-77 7.09316949057566E-5 0.0 0.0 0.7707083309984983 0.0013477022032093754 78-79 7.09316949057566E-5 0.0 0.0 0.8427749330227471 0.0013477022032093754 80-81 7.09316949057566E-5 0.0 0.0 0.9366884970779689 0.0013831680506622536 82-83 7.09316949057566E-5 0.0 0.0 1.0917806479894057 0.001418633898115132 84-85 7.09316949057566E-5 0.0 0.0 1.2535758440694365 0.001418633898115132 86-87 7.09316949057566E-5 0.0 0.0 1.4850259645469204 0.0015604972879266453 88-89 7.09316949057566E-5 0.0 0.0 1.766589327475321 0.0017732923726439152 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 955 0.0 25.367958 2 GAGTACT 8050 0.0 22.594275 12-13 GTACATG 8905 0.0 22.304876 1 TACATGG 8850 0.0 21.738546 2 ACATGGG 8865 0.0 20.896511 3 CATGGGG 3055 0.0 20.831469 4 ATAACGC 115 0.003723971 20.651905 3 GTACTTT 8835 0.0 20.479244 14-15 GTACCTG 1325 0.0 20.441671 1 AGTACTT 8265 0.0 20.42642 12-13 TAACGCA 140 4.8575617E-4 20.353989 4 GTGTAGC 690 0.0 19.282635 1 GTCCTAA 350 6.366463E-11 19.007168 1 ACTTTTT 10050 0.0 18.097902 16-17 GGTATAC 220 2.9085088E-5 17.279243 1 TACTTTT 9665 0.0 17.221924 14-15 GTATAGG 395 3.583409E-10 16.841795 1 CATGGGA 5870 0.0 16.505108 4 CATGGGT 955 0.0 16.41107 4 CCTATAC 325 1.9131949E-7 16.076714 3 >>END_MODULE