FastQCFastQC Report
Thu 26 May 2016
SRR936511_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936511_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1236999
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT49070.396685850190663No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36530.29531147559537235No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29710.24017804379793356No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT29270.2366210481980988No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT20440.1652386137741421No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC18920.15295081079289474No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16310.13185135962114763No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT15800.1277284783577028No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC12940.10460800695877685No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT12550.10145521540437785No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGAC600.003948309531.6711852
GTACATG49300.027.2684151
GTATCGA700.00838928727.1445331
TACATGG52400.024.5693422
ACATGGG50750.024.5266553
GAGTACT41550.022.81057412-13
ATACCGT1553.898785E-521.4572756
GTGTAGC6900.021.3418961
GTACACG2257.148083E-821.1124131
GTACTTT44650.021.0140614-15
GCGCCTA804.9860115E-520.78463412-13
CATGGGG23150.020.3177154
GGACCGT1200.00475643119.7968926
AGAGTAC70400.019.47029710-11
AGTACTT44800.019.35303912-13
GTAGCCC6050.018.8464243
TGCACCG3054.5511115E-918.6927015
GTATCAA109050.018.6875361
CATGGGA28100.018.4293864
ACTTTTT52600.018.15299616-17
Produced by FastQC (version 0.11.5)