##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936511_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1236999 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.55023973341935 31.0 31.0 34.0 30.0 34.0 2 31.630238989683903 33.0 31.0 34.0 30.0 34.0 3 31.649756386221817 33.0 31.0 34.0 30.0 34.0 4 35.274994563455586 37.0 35.0 37.0 33.0 37.0 5 35.289496596197736 37.0 35.0 37.0 33.0 37.0 6 35.28487492714222 37.0 35.0 37.0 33.0 37.0 7 35.199943573115256 37.0 35.0 37.0 32.0 37.0 8 35.169034089760785 37.0 35.0 37.0 32.0 37.0 9 36.70227057580483 39.0 37.0 39.0 32.0 39.0 10-11 36.579393758604496 39.0 37.0 39.0 32.0 39.0 12-13 36.5006511727172 39.0 37.0 39.0 32.0 39.0 14-15 37.61475191168303 40.0 37.0 41.0 32.0 41.0 16-17 37.527375931589276 40.0 37.0 41.0 32.0 41.0 18-19 37.49920493064263 40.0 37.0 41.0 32.0 41.0 20-21 37.37047928090483 40.0 37.0 41.0 32.0 41.0 22-23 37.47547006909464 40.0 37.0 41.0 32.0 41.0 24-25 37.51499879951399 40.0 37.0 41.0 32.0 41.0 26-27 37.433874239186935 40.0 37.0 41.0 32.0 41.0 28-29 37.303165564402235 40.0 37.0 41.0 31.0 41.0 30-31 37.111748271421405 39.0 36.5 41.0 31.0 41.0 32-33 36.927906166456076 39.0 36.0 41.0 30.5 41.0 34-35 36.737910459103034 39.0 36.0 41.0 30.0 41.0 36-37 36.54946244903998 39.0 35.0 41.0 30.0 41.0 38-39 36.356384281636444 39.0 35.0 40.0 30.0 41.0 40-41 36.167085017853694 39.0 35.0 40.0 30.0 41.0 42-43 35.94054643536494 38.5 35.0 40.0 29.0 41.0 44-45 35.685350998666934 38.0 35.0 40.0 27.5 41.0 46-47 35.526314087561914 38.0 35.0 40.0 27.0 41.0 48-49 35.377080337170845 39.0 35.0 40.0 27.0 41.0 50-51 34.412649484761104 38.0 34.0 39.5 25.0 40.5 52-53 34.531438990653996 38.0 33.5 39.5 25.5 40.5 54-55 34.98095592639929 38.0 34.0 40.0 26.0 41.0 56-57 34.87569230047882 38.0 34.0 40.0 26.0 41.0 58-59 34.65673052282176 38.0 34.0 40.0 26.0 41.0 60-61 34.3561268036595 38.0 34.0 40.0 24.5 41.0 62-63 34.01444099793129 37.0 33.5 40.0 23.0 41.0 64-65 33.67799731446833 37.0 33.0 40.0 23.0 41.0 66-67 33.20260283153018 36.0 32.5 39.0 22.0 41.0 68-69 32.81734140447971 36.0 32.0 39.0 21.0 40.5 70-71 32.411623614893784 35.0 32.0 38.5 20.0 40.0 72-73 32.022456364152276 35.0 31.0 37.5 19.5 40.0 74-75 31.600931771165538 35.0 31.0 37.0 18.0 39.0 76-77 31.1403485370643 35.0 31.0 36.5 15.5 39.0 78-79 30.6442183865953 34.5 30.0 36.0 11.0 38.5 80-81 30.182897884315185 34.0 30.0 36.0 7.0 37.5 82-83 29.76823101716331 34.0 29.5 35.0 6.5 37.0 84-85 29.40371132070438 34.0 29.0 35.0 2.0 36.5 86-87 29.061027939392027 34.0 29.0 35.0 2.0 36.0 88-89 28.658487597807273 34.0 29.0 35.0 2.0 36.0 90-91 28.24881305482058 34.0 28.5 35.0 2.0 35.0 92-93 27.801810268237887 34.0 27.0 35.0 2.0 35.0 94-95 27.30958634566398 33.0 26.0 35.0 2.0 35.0 96-97 26.741106904694345 33.0 25.0 35.0 2.0 35.0 98-99 26.02537148372796 33.0 24.5 35.0 2.0 35.0 100-101 23.132780624721605 29.5 10.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5635684271075299 1101 2 0.48598934164427376 1101 3 0.47907210754260987 1101 4 0.5330009372571354 1101 5 0.5097706731187657 1101 6 0.5403708582369902 1101 7 0.46739069714114834 1101 8 0.3860589987460372 1101 9 0.41594452812648086 1101 10-11 0.6593686124928837 1101 12-13 0.625622623918261 1101 14-15 0.7364839564283372 1101 16-17 0.8484700790730884 1101 18-19 0.7695758607124148 1101 20-21 0.7626158203396827 1101 22-23 0.7474748956258637 1101 24-25 0.6951073834309511 1101 26-27 0.5808322016171914 1101 28-29 0.5511518074190391 1101 30-31 0.5499084418988502 1101 32-33 0.5132340354599805 1101 34-35 0.5048720937369922 1101 36-37 0.45306239447779006 1101 38-39 0.454418436268071 1101 40-41 0.5368491519429668 1101 42-43 0.5491088213782973 1101 44-45 0.4607566748026457 1101 46-47 0.636745446660818 1101 48-49 0.8180104291784218 1101 50-51 0.6057358225414973 1101 52-53 0.7195340830233405 1101 54-55 0.6948636638334875 1101 56-57 0.6580629247584824 1101 58-59 0.5728898694042712 1101 60-61 0.5362422813143368 1101 62-63 0.6983717691952478 1101 64-65 0.6124181310490471 1101 66-67 0.6749083017226098 1101 68-69 0.6248617526042537 1101 70-71 0.3655933443972401 1101 72-73 0.20829286464510588 1101 74-75 0.4831071602313699 1101 76-77 0.2936417477944566 1101 78-79 0.25883366312673317 1101 80-81 0.3160783151651181 1101 82-83 0.2384254643930106 1101 84-85 0.07954838634002925 1101 86-87 0.26680172571300176 1101 88-89 0.24149032575137142 1101 90-91 0.24608302678226224 1101 92-93 0.20985136486936895 1101 94-95 0.26727961875313966 1101 96-97 0.1734831885517707 1101 98-99 0.033669152445309436 1101 100-101 0.25530593822728775 1102 1 0.22424786268900831 1102 2 0.32280868978040544 1102 3 0.370071776586812 1102 4 0.23481174959091788 1102 5 0.254647405973941 1102 6 0.2435519228035261 1102 7 0.4377280895110687 1102 8 0.42120698043156324 1102 9 0.5613607109918561 1102 10-11 0.4966314175853199 1102 12-13 0.4788623795665359 1102 14-15 0.39541793749322096 1102 16-17 0.3295809952968227 1102 18-19 0.39101620096989365 1102 20-21 0.3858117342657792 1102 22-23 0.29532757188363945 1102 24-25 0.2942905496064725 1102 26-27 0.11002020117591371 1102 28-29 -0.09066592562106734 1102 30-31 0.40916552153539243 1102 32-33 0.3078335035497304 1102 34-35 0.31582411149589973 1102 36-37 0.17068428264013136 1102 38-39 -0.019864631731834947 1102 40-41 -1.256343048225972E-4 1102 42-43 -0.08175860026970128 1102 44-45 -0.10857935802943786 1102 46-47 0.11554344219569401 1102 48-49 0.3074674632589236 1102 50-51 0.3935405776699312 1102 52-53 0.4910805354867378 1102 54-55 0.4009392027080949 1102 56-57 0.34006916768004203 1102 58-59 0.2647075230784779 1102 60-61 0.17538042321580605 1102 62-63 0.30376511849171095 1102 64-65 0.35518373101158573 1102 66-67 0.34428108096557253 1102 68-69 0.41897371566418684 1102 70-71 0.42403640769868645 1102 72-73 0.16627239141800132 1102 74-75 0.37855786726247587 1102 76-77 0.2555165528519723 1102 78-79 0.13518734772824104 1102 80-81 0.2698165283371594 1102 82-83 0.25864868891834547 1102 84-85 -0.014005356674779534 1102 86-87 0.16915766271870325 1102 88-89 0.06908854700285971 1102 90-91 -0.004549321011804608 1102 92-93 0.16383382659318002 1102 94-95 0.38868364911218123 1102 96-97 0.19862412254553163 1102 98-99 0.03705470324861082 1102 100-101 0.20724869636685384 1103 1 0.3315647739944403 1103 2 0.06711068853118718 1103 3 0.24316359420080502 1103 4 0.4544494760119022 1103 5 0.4505632398277868 1103 6 0.4144591682751084 1103 7 0.4555681113724077 1103 8 0.4683985794321899 1103 9 0.2145809639587526 1103 10-11 0.5037992033442222 1103 12-13 0.2987813755065716 1103 14-15 0.609719741053496 1103 16-17 0.5025377410807153 1103 18-19 0.6221239070910727 1103 20-21 0.6246564016611558 1103 22-23 0.3429359026144283 1103 24-25 0.5625517925796117 1103 26-27 0.4418618235993961 1103 28-29 0.585658637152207 1103 30-31 0.5525458307171434 1103 32-33 0.4344955507077586 1103 34-35 0.6701889615896022 1103 36-37 0.6064010221779128 1103 38-39 0.6465594781994568 1103 40-41 0.6693118266570721 1103 42-43 0.5771762451394196 1103 44-45 0.6539237650186536 1103 46-47 0.8455867744880052 1103 48-49 0.9009765981746085 1103 50-51 0.8549293581196409 1103 52-53 0.9616612272418905 1103 54-55 0.9883150615463236 1103 56-57 0.8542247735513726 1103 58-59 0.6639917594932214 1103 60-61 0.8131696955455965 1103 62-63 1.0889181478483607 1103 64-65 0.9898454658648035 1103 66-67 1.0402164338700004 1103 68-69 1.0300285251582508 1103 70-71 0.9748468386793192 1103 72-73 0.6566807299564097 1103 74-75 0.7157004734461125 1103 76-77 0.747935743982513 1103 78-79 0.6269030722753968 1103 80-81 0.8041409739961232 1103 82-83 0.5142775927284617 1103 84-85 0.6809194949804365 1103 86-87 0.5461644163102051 1103 88-89 0.6023492044044829 1103 90-91 0.675276959437916 1103 92-93 0.6203600211590761 1103 94-95 0.7344107337467847 1103 96-97 0.8748145894412254 1103 98-99 0.8245470908188786 1103 100-101 0.918039022725381 1104 1 0.2700188389140372 1104 2 0.34967620371879704 1104 3 0.262949180859529 1104 4 0.26173952961523383 1104 5 0.3765675578742389 1104 6 0.3810002641655146 1104 7 0.4660576140525734 1104 8 0.3833799671629805 1104 9 0.45302981744296744 1104 10-11 0.6127483546247419 1104 12-13 0.5319352245238491 1104 14-15 0.3898401624352701 1104 16-17 0.15209328009203205 1104 18-19 0.21010987344008925 1104 20-21 -0.03635294169798442 1104 22-23 0.11991382846314735 1104 24-25 0.14568833218652344 1104 26-27 -0.09416948223740462 1104 28-29 0.055667943286806576 1104 30-31 0.1492711880727171 1104 32-33 -0.06109054964959881 1104 34-35 0.16708480733110775 1104 36-37 0.09775450639471472 1104 38-39 -0.00749352954324678 1104 40-41 -0.11912213701177876 1104 42-43 0.13827194942768273 1104 44-45 0.02251667746654107 1104 46-47 0.03102416281438991 1104 48-49 0.1895758387940205 1104 50-51 0.14439239415299454 1104 52-53 0.2781738463604171 1104 54-55 0.10164963569760488 1104 56-57 0.23189786765500742 1104 58-59 0.36942896169691153 1104 60-61 0.21582045492618107 1104 62-63 0.2375959322438348 1104 64-65 0.20522273656164458 1104 66-67 0.11126616585332982 1104 68-69 -0.02631528067855271 1104 70-71 0.015077630817494025 1104 72-73 -0.0716434213241115 1104 74-75 0.06801751483979857 1104 76-77 0.06795547298787241 1104 78-79 0.19318471313305352 1104 80-81 0.23754087721232153 1104 82-83 0.16633945991130972 1104 84-85 0.06722067872075854 1104 86-87 -0.0026367674301148725 1104 88-89 0.052155822927414164 1104 90-91 -0.09139851703617552 1104 92-93 -0.17444436389154916 1104 94-95 -0.27197159948728 1104 96-97 -0.08223964671124762 1104 98-99 -0.17857958428534815 1104 100-101 0.07494808317802892 1105 1 0.1617569931472964 1105 2 0.08601952831019233 1105 3 0.09474124920387439 1105 4 0.14164407392473066 1105 5 0.0680118279186388 1105 6 0.19036309706577725 1105 7 0.20983798552833122 1105 8 0.37137049471763817 1105 9 0.37150967789368394 1105 10-11 0.28293172329510696 1105 12-13 0.11184517998785282 1105 14-15 0.2154174467011316 1105 16-17 0.12856060787015622 1105 18-19 0.12206271157234738 1105 20-21 0.10174721657828201 1105 22-23 -0.12324407743467702 1105 24-25 -0.20454535290841136 1105 26-27 -0.23896978321459983 1105 28-29 -0.15932179562864235 1105 30-31 -0.1973033865940863 1105 32-33 -0.3017181923861685 1105 34-35 -0.22349159396596008 1105 36-37 -0.27597620311367166 1105 38-39 -0.41139325353780976 1105 40-41 -0.39840571477939335 1105 42-43 -0.46498823460276384 1105 44-45 -0.05403050140245824 1105 46-47 -0.1517624428232267 1105 48-49 -0.2994749825497607 1105 50-51 -0.2321299957784504 1105 52-53 -0.025810189271304296 1105 54-55 -0.32554236632353906 1105 56-57 -0.33560372552781814 1105 58-59 -0.1035759584318896 1105 60-61 -0.03331165804556946 1105 62-63 -0.14181850040209554 1105 64-65 -0.02348153689762711 1105 66-67 0.032799306798182215 1105 68-69 0.16191081490656956 1105 70-71 0.04600859435765159 1105 72-73 -0.0791503013511381 1105 74-75 0.12319337553328324 1105 76-77 0.20310417553561422 1105 78-79 0.20463826257619644 1105 80-81 0.27120685030060443 1105 82-83 0.03570523238468937 1105 84-85 -0.09450465327002533 1105 86-87 -0.4799537927137365 1105 88-89 -0.41806765409766555 1105 90-91 -0.3401646458413943 1105 92-93 -0.21761093494896855 1105 94-95 -0.16937245379158128 1105 96-97 -0.27487674726841504 1105 98-99 -0.13523440856724633 1105 100-101 -0.21135573632925286 1106 1 0.33032108712575337 1106 2 0.25905513297562877 1106 3 0.349931270968483 1106 4 0.32960099116341723 1106 5 0.29815162366616477 1106 6 0.4150652288811685 1106 7 0.4625062426855351 1106 8 0.5345980743816838 1106 9 0.4733499033526911 1106 10-11 0.5019747083947266 1106 12-13 0.4361203906580897 1106 14-15 0.5425196147908693 1106 16-17 0.5220831956261662 1106 18-19 0.4210853969900583 1106 20-21 0.21319491176216587 1106 22-23 0.39661961473564844 1106 24-25 0.38659219662001476 1106 26-27 0.27327280844788504 1106 28-29 0.10474323311180456 1106 30-31 0.5319208307171479 1106 32-33 0.5175605759602817 1106 34-35 0.4659023454279776 1106 36-37 0.3048353656122629 1106 38-39 0.38748119537117276 1106 40-41 0.38005046302071577 1106 42-43 0.3702791491798152 1106 44-45 0.3034060882509735 1106 46-47 0.4777142997405335 1106 48-49 0.648975335548343 1106 50-51 0.1822241813519625 1106 52-53 0.36721362623178777 1106 54-55 0.46446405144531155 1106 56-57 0.39690785435945486 1106 58-59 0.4171893605033219 1106 60-61 0.6393123723132703 1106 62-63 0.7303196629998752 1106 64-65 0.7818246325314746 1106 66-67 0.5942442116477835 1106 68-69 0.48443509081482006 1106 70-71 0.27949330332578626 1106 72-73 0.17423123500691773 1106 74-75 0.5056278724360084 1106 76-77 0.48140165307342286 1106 78-79 0.4083361530834786 1106 80-81 0.6196428679355144 1106 82-83 0.3130086533345242 1106 84-85 0.4629239141723538 1106 86-87 0.39774585570414445 1106 88-89 0.2551301387479157 1106 90-91 0.37756862610457986 1106 92-93 0.5764048443914014 1106 94-95 0.4071664155649657 1106 96-97 0.35258921065334903 1106 98-99 0.2553583281926173 1106 100-101 0.2864765227253798 1107 1 0.21788290568946422 1107 2 0.12833154549401016 1107 3 0.17867714177484473 1107 4 0.14589245017699426 1107 5 0.10689358009243222 1107 6 0.22989237558449815 1107 7 0.15771327520353395 1107 8 0.3393492725638865 1107 9 0.323259681665121 1107 10-11 0.34291900485651183 1107 12-13 0.24142413700626264 1107 14-15 -0.06087670664656031 1107 16-17 0.01655711726218101 1107 18-19 0.027003987431335474 1107 20-21 0.060115798321525915 1107 22-23 -0.0030803623509143563 1107 24-25 0.2489545966123785 1107 26-27 0.1407400135804977 1107 28-29 0.26010295032170916 1107 30-31 0.24767776203410108 1107 32-33 0.07672440231456079 1107 34-35 0.2854351021062911 1107 36-37 0.32068741159190495 1107 38-39 0.04119400876026447 1107 40-41 0.11354741266841728 1107 42-43 0.2715120595691545 1107 44-45 0.35523283874265843 1107 46-47 0.23902840964868943 1107 48-49 0.23254914070768962 1107 50-51 0.3365832254794441 1107 52-53 0.5065231916401274 1107 54-55 0.6430433224158847 1107 56-57 0.20905818564966694 1107 58-59 0.34345162874337376 1107 60-61 0.30769277368043646 1107 62-63 0.29741846290822593 1107 64-65 0.4231880540815638 1107 66-67 0.41324888503572765 1107 68-69 0.39069881501333015 1107 70-71 0.4805289432791966 1107 72-73 0.25710212252099396 1107 74-75 0.4696066249899076 1107 76-77 0.33597646546959226 1107 78-79 0.03558572823003203 1107 80-81 0.1767904305178618 1107 82-83 0.34408501238819866 1107 84-85 0.2675426440037505 1107 86-87 0.03806206123774203 1107 88-89 -0.1454428167045272 1107 90-91 -0.14846319494141724 1107 92-93 -0.13535536163865558 1107 94-95 0.19069164899568136 1107 96-97 0.09853997538955639 1107 98-99 0.10153452780690841 1107 100-101 -0.2255154432481561 1108 1 0.19131564812031598 1108 2 0.15499530391580052 1108 3 0.23435240538961466 1108 4 0.19067325223567622 1108 5 0.16064191115645343 1108 6 0.1562948326107687 1108 7 0.3090777267570246 1108 8 0.23352969831330483 1108 9 0.34462029962308804 1108 10-11 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4478.0 13 5517.0 14 6232.0 15 7428.0 16 9233.0 17 11513.0 18 9447.0 19 7430.0 20 7462.0 21 7862.0 22 8931.0 23 10157.0 24 11604.0 25 13426.0 26 15500.0 27 18372.0 28 21891.0 29 26885.0 30 32548.0 31 40317.0 32 51095.0 33 66133.0 34 88251.0 35 121163.0 36 172641.0 37 231686.0 38 184773.0 39 26786.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.317596559583514 18.64116808575492 14.457316989360677 22.583918365300885 2 15.40656309222739 20.26517234019158 40.27590924084938 24.052355326731647 3 20.008295487529683 24.2225810285596 29.582630251686385 26.18649323222433 4 12.728120964853343 16.47920542630958 37.86733226120219 32.925341347634884 5 13.77352860549573 37.47659976727129 34.080927809625 14.668943817607975 6 31.610805074978625 36.237553774246436 17.597432585555545 14.554208565219396 7 28.602530945591866 32.73890417315788 20.540790942729938 18.11777393852032 8 26.268575614820712 34.0533936227159 19.834608499056362 19.843422263407028 9 26.031706496564755 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29.008190535551808 22.311499046263208 34-35 22.85285713766023 26.697445469820696 28.606219005116014 21.843478387403056 36-37 22.604425484511044 26.438153120325108 28.923006529131694 22.034414866032144 38-39 22.20371415313525 26.973810790316556 28.868640046305654 21.95383501024254 40-41 22.414994583582594 26.826666558877264 28.830781419263047 21.927557438277095 42-43 22.168281388445145 27.038276343786986 28.201619787995007 22.591822479772862 44-45 22.42625050071768 26.82908984785054 28.25927292761492 22.485386723816863 46-47 22.818388552183652 27.247820712747995 27.855385707367986 22.07840502770037 48-49 22.747016353366593 27.21955580993587 27.760383445985546 22.273044390711995 50-51 22.24670876717567 27.442670966453658 28.05150204877197 22.259118217598704 52-53 22.797431928854582 27.578914265180067 27.39770380517713 22.225950000788224 54-55 22.231434089694574 27.21675663395937 27.824268289823767 22.72754098652229 56-57 22.636579630053298 27.356872211474798 27.46677617990136 22.539771978570545 58-59 23.123934857760055 27.048306929124184 27.401643108226537 22.426115104889224 60-61 22.56776765398873 27.241948138408063 28.227222182025287 21.96306202557792 62-63 22.353135671047188 27.41978121119619 28.03001151179636 22.197071605960264 64-65 22.05233776171003 27.708552532388243 27.72508467163493 22.514025034266798 66-67 22.31545618385615 27.46304353274962 27.38518086696804 22.836319416426193 68-69 22.236945965793108 27.310477518366717 27.76548028429181 22.687096231548367 70-71 22.54704859615555 27.303897956930406 27.47371899168609 22.67533445522795 72-73 22.47104524941573 27.108494862348454 27.95272964394824 22.46773024428758 74-75 22.1249021497933 28.055190751295505 27.32806667399869 22.491840424912503 76-77 22.355349947479375 27.498530311962003 27.378327216034844 22.767792524523777 78-79 22.405813666143718 27.50005156684337 27.47331769745753 22.62081706955538 80-81 22.529000677609556 27.680046926990098 27.271741658828645 22.5192107365717 82-83 22.521927935557002 27.603337001300975 27.50805881203951 22.36667625110251 84-85 22.40707649765114 27.721524616541476 27.326665426878076 22.544733458929308 86-87 22.442393928662103 27.749154647377587 27.195086847752435 22.61336457620787 88-89 22.23359620162993 27.682352246751744 26.973270878866312 23.110780672752014 90-91 22.34619815598323 27.40675082724383 27.281759470693352 22.965291546079595 92-93 22.724707221795008 27.465235746797696 27.426907536021645 22.383149495385656 94-95 22.308878354679795 27.418445897816735 27.437931399713943 22.834744347789528 96-97 21.96516018077669 27.357978354178552 27.796861917835464 22.87999954720929 98-99 22.081314345750965 27.631801369635884 27.10187020730564 23.185014077307507 100-101 22.046236686141615 27.382135038887874 27.26802780964277 23.303600465327744 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 86.0 1 68.5 2 81.0 3 121.5 4 124.5 5 125.0 6 192.0 7 377.0 8 646.5 9 829.5 10 807.0 11 766.5 12 827.0 13 909.0 14 1067.5 15 1150.5 16 1207.0 17 1320.0 18 1418.5 19 1567.0 20 1781.5 21 2070.5 22 2481.5 23 3008.5 24 3657.5 25 4626.0 26 5893.0 27 7493.5 28 9181.0 29 11293.0 30 13983.0 31 17069.5 32 20554.5 33 22857.0 34 24962.0 35 27037.5 36 29374.5 37 32538.0 38 34557.5 39 35593.5 40 36818.0 41 39130.5 42 41134.0 43 42895.5 44 44538.5 45 46419.0 46 47585.0 47 50980.5 48 59101.5 49 57915.5 50 49373.0 51 44590.5 52 41769.0 53 40763.5 54 39123.0 55 35755.5 56 32300.5 57 28503.5 58 25470.5 59 22303.5 60 18381.5 61 15134.5 62 12515.5 63 9750.5 64 7140.5 65 5582.5 66 4347.0 67 3432.5 68 2618.5 69 1815.0 70 1343.5 71 1026.5 72 818.0 73 746.5 74 596.5 75 477.5 76 379.0 77 250.5 78 171.0 79 110.5 80 74.0 81 51.5 82 32.5 83 22.0 84 11.5 85 5.0 86 5.0 87 4.5 88 4.0 89 3.5 90 2.0 91 2.5 92 3.0 93 3.0 94 2.0 95 1.5 96 1.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01390461916299043 2 0.011560235699462976 3 0.014713027253861966 4 0.01697656990830227 5 0.029587736125898243 6 0.04688766927054913 7 0.005658856636100757 8 0.02400972029888464 9 0.03290220929847154 10-11 0.038763167957290186 12-13 0.03116413190309774 14-15 0.026596626189673556 16-17 0.027566715898719403 18-19 0.02259500613985945 20-21 0.05246568509756273 22-23 0.012328223385790933 24-25 0.0024656446771581866 26-27 0.001455134563568766 28-29 1.212612136307305E-4 30-31 0.002708167104419648 32-33 0.0018189182044609576 34-35 4.042040454357683E-5 36-37 3.637836408921915E-4 38-39 8.084080908715366E-5 40-41 0.0015359753726559198 42-43 0.0037995180270962224 44-45 0.001980599822635265 46-47 9.70089709045844E-4 48-49 4.85044854522922E-4 50-51 0.0031123711498554163 52-53 0.0031932119589425704 54-55 0.005497175017926449 56-57 8.084080908715367E-4 58-59 0.006063060681536525 60-61 0.002708167104419648 62-63 5.658856636100757E-4 64-65 0.0011721917317637283 66-67 0.016653206671953656 68-69 0.04227974315258137 70-71 0.056386464338289685 72-73 0.016046900603800002 74-75 0.03354893577116877 76-77 0.027647556707806553 78-79 0.05978177831995014 80-81 0.08379149861883477 82-83 0.05008088122949169 84-85 0.01851254528095819 86-87 0.019159271753655418 88-89 0.020856928744485647 90-91 0.04151175546625341 92-93 0.025222332435191945 94-95 0.014713027253861966 96-97 0.018148761640066 98-99 0.04947457516133805 100-101 0.07122075280578238 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1236999.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.01857933752274 #Duplication Level Percentage of deduplicated Percentage of total 1 83.74689583702335 55.288510870877786 2 10.134046276609581 13.380706762449531 3 2.756244880942931 5.458901140385155 4 1.1052313164547332 2.9186320540672606 5 0.5743179904836626 1.8957828909856156 6 0.36506008910702875 1.44604490734053 7 0.23189618522587316 1.0716619691682223 8 0.15990199126679022 0.8445201837339567 9 0.12122818224332128 0.7202981130636934 >10 0.7276136731962926 8.82127865273508 >50 0.050398037133207944 2.2490929942326208 >100 0.02410637127334545 3.077424684598917 >500 0.0015907679007517586 0.6995155623075947 >1k 0.0014684011391554695 2.1276292140541777 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4907 0.396685850190663 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3653 0.29531147559537235 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2971 0.24017804379793356 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2927 0.2366210481980988 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2044 0.1652386137741421 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1892 0.15295081079289474 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1631 0.13185135962114763 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1580 0.1277284783577028 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1294 0.10460800695877685 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1255 0.10145521540437785 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 8.084080908715367E-5 0.0 18-19 0.0 0.0 0.0 8.084080908715367E-5 0.0 20-21 0.0 0.0 0.0 8.084080908715367E-5 0.0 22-23 0.0 0.0 0.0 2.0210202271788417E-4 4.0420404543576836E-5 24-25 0.0 0.0 0.0 2.8294283180503783E-4 8.084080908715367E-5 26-27 0.0 0.0 0.0 4.4462444997934514E-4 8.084080908715367E-5 28-29 0.0 8.084080908715367E-5 0.0 7.275672817843831E-4 8.084080908715367E-5 30-31 0.0 8.084080908715367E-5 0.0 0.0016168161817430734 8.084080908715367E-5 32-33 0.0 8.084080908715367E-5 0.0 0.003152791554398993 8.084080908715367E-5 34-35 0.0 8.084080908715367E-5 0.0 0.0052950729952085645 1.6168161817430734E-4 36-37 0.0 8.084080908715367E-5 0.0 0.007639456458736022 1.6168161817430734E-4 38-39 0.0 8.084080908715367E-5 0.0 0.01022636234952494 1.6168161817430734E-4 40-41 0.0 8.084080908715367E-5 0.0 0.013581255926641816 1.6168161817430734E-4 42-43 0.0 8.084080908715367E-5 0.0 0.020291043080875572 1.6168161817430734E-4 44-45 0.0 8.084080908715367E-5 0.0 0.0319321195894257 1.6168161817430734E-4 46-47 0.0 8.084080908715367E-5 0.0 0.044502865402478095 1.6168161817430734E-4 48-49 0.0 8.084080908715367E-5 0.0 0.0682296428695577 1.6168161817430734E-4 50-51 0.0 8.084080908715367E-5 0.0 0.09175431831391942 1.6168161817430734E-4 52-53 0.0 8.084080908715367E-5 0.0 0.11038812480850833 1.6168161817430734E-4 54-55 0.0 8.084080908715367E-5 0.0 0.14183519954341112 1.6168161817430734E-4 56-57 0.0 8.084080908715367E-5 0.0 0.1832256937960338 1.6168161817430734E-4 58-59 0.0 8.084080908715367E-5 0.0 0.23427666473457132 1.6168161817430734E-4 60-61 0.0 8.084080908715367E-5 0.0 0.28831874560933357 1.6168161817430734E-4 62-63 0.0 8.084080908715367E-5 0.0 0.3700488035964459 1.6168161817430734E-4 64-65 0.0 8.084080908715367E-5 0.0 0.4371062547342399 1.6168161817430734E-4 66-67 0.0 8.084080908715367E-5 0.0 0.5383997885204435 2.0210202271788417E-4 68-69 0.0 8.084080908715367E-5 0.0 0.6726359520096621 2.42522427261461E-4 70-71 0.0 8.084080908715367E-5 0.0 0.7570337566966505 2.42522427261461E-4 72-73 0.0 8.084080908715367E-5 0.0 0.8716256035776908 2.42522427261461E-4 74-75 0.0 8.084080908715367E-5 0.0 1.017583684384547 2.42522427261461E-4 76-77 0.0 8.084080908715367E-5 0.0 1.1345603351336582 2.42522427261461E-4 78-79 0.0 8.084080908715367E-5 0.0 1.2400575909923939 2.42522427261461E-4 80-81 0.0 8.084080908715367E-5 0.0 1.4003244950076759 2.8294283180503783E-4 82-83 0.0 8.084080908715367E-5 0.0 1.6126124596705413 3.233632363486147E-4 84-85 0.0 8.084080908715367E-5 0.0 1.85363933196389 3.233632363486147E-4 86-87 0.0 8.084080908715367E-5 0.0 2.1906646650482338 3.233632363486147E-4 88-89 0.0 8.084080908715367E-5 0.0 2.5654022355717343 3.233632363486147E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCGAC 60 0.0039483095 31.671185 2 GTACATG 4930 0.0 27.268415 1 GTATCGA 70 0.008389287 27.144533 1 TACATGG 5240 0.0 24.569342 2 ACATGGG 5075 0.0 24.526655 3 GAGTACT 4155 0.0 22.810574 12-13 ATACCGT 155 3.898785E-5 21.457275 6 GTGTAGC 690 0.0 21.341896 1 GTACACG 225 7.148083E-8 21.112413 1 GTACTTT 4465 0.0 21.01406 14-15 GCGCCTA 80 4.9860115E-5 20.784634 12-13 CATGGGG 2315 0.0 20.317715 4 GGACCGT 120 0.004756431 19.796892 6 AGAGTAC 7040 0.0 19.470297 10-11 AGTACTT 4480 0.0 19.353039 12-13 GTAGCCC 605 0.0 18.846424 3 TGCACCG 305 4.5511115E-9 18.692701 5 GTATCAA 10905 0.0 18.687536 1 CATGGGA 2810 0.0 18.429386 4 ACTTTTT 5260 0.0 18.152996 16-17 >>END_MODULE