FastQCFastQC Report
Thu 26 May 2016
SRR936511_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936511_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1236999
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT53590.43322589589805655No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45660.36911913429194365No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40680.32886041136654115No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT28310.22886033052573204No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23630.19102683187294411No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC19730.1594989163289542No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT19700.15925639390169274No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC15060.12174625848525343No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT14790.11956355663990029No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT14530.11746169560363429No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12380.10008092164989625No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGCG350.00839520127.1416736-37
CGAATAG908.94178E-426.3923
TAAGGTG6000.024.5425765
GTAAGGT4100.023.171594
GTACATG46450.023.118271
CGGTTAC753.0360634E-522.16569728-29
GAGTACT55450.022.05808412-13
TACATGG48150.022.0042252
GTCTACG1100.002872150421.5978181
ACATGGG48000.021.3775223
ATTCGCG1005.392503E-721.37449580-81
GTACTTT57550.021.1706514-15
AGTACTT57500.020.4042812-13
CATGGGG22050.020.0347564
AGAGTAC84450.019.79863210-11
GTATCAA115050.019.6586171
AGGCGAC2451.7555976E-719.3884745
GGCCGAT3003.7180143E-918.9991687
TACTTTT63950.018.42057814-15
GTATAGC4650.018.3929821
Produced by FastQC (version 0.11.5)