##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936511_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1236999 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.961325756932705 31.0 31.0 34.0 30.0 34.0 2 32.08794429098164 33.0 31.0 34.0 30.0 34.0 3 32.20676330376985 34.0 31.0 34.0 30.0 34.0 4 35.92624892986979 37.0 35.0 37.0 35.0 37.0 5 35.79453257439982 37.0 35.0 37.0 35.0 37.0 6 35.843737141258806 37.0 35.0 37.0 35.0 37.0 7 35.77727548688398 37.0 35.0 37.0 33.0 37.0 8 35.74267723741086 37.0 35.0 37.0 33.0 37.0 9 37.37041662927779 39.0 37.0 39.0 34.0 39.0 10-11 37.27048000847212 39.0 37.0 39.0 34.0 39.0 12-13 37.149841673275404 39.0 37.0 39.0 33.0 39.0 14-15 38.25734054756714 40.0 38.0 41.0 33.0 41.0 16-17 38.24030860170461 40.0 37.5 41.0 33.0 41.0 18-19 38.38607145195752 40.0 38.0 41.0 33.5 41.0 20-21 38.39506297094824 40.0 38.0 41.0 34.0 41.0 22-23 38.30452086056658 40.0 38.0 41.0 34.0 41.0 24-25 38.18827460652757 40.0 37.5 41.0 33.0 41.0 26-27 37.995834677311784 40.0 37.0 41.0 33.0 41.0 28-29 38.016740918949814 40.0 37.0 41.0 33.0 41.0 30-31 37.89798051574819 40.0 37.0 41.0 33.0 41.0 32-33 37.704424174958916 40.0 37.0 41.0 33.0 41.0 34-35 37.51379144203027 40.0 36.0 41.0 32.5 41.0 36-37 37.33328280782766 39.5 36.0 41.0 32.0 41.0 38-39 37.137042147972636 39.0 36.0 41.0 31.0 41.0 40-41 36.917249326798164 39.0 36.0 41.0 31.0 41.0 42-43 36.659778625528396 39.0 35.0 40.5 30.5 41.0 44-45 36.37528324598484 39.0 35.0 40.0 30.0 41.0 46-47 36.00231043032371 39.0 35.0 40.0 29.5 41.0 48-49 35.603730479976136 38.5 35.0 40.0 28.0 41.0 50-51 35.30769588334348 38.0 35.0 40.0 27.0 41.0 52-53 35.052494383584786 38.0 34.0 40.0 26.5 41.0 54-55 34.741218060806844 38.0 34.0 40.0 26.0 41.0 56-57 34.407240830429124 38.0 33.5 40.0 25.5 41.0 58-59 34.038878770314284 37.0 33.0 40.0 24.5 41.0 60-61 33.653886138954036 37.0 33.0 39.5 23.0 41.0 62-63 33.33679331996226 36.0 32.0 39.0 23.0 40.0 64-65 33.04086300797333 36.0 32.0 39.0 22.0 40.0 66-67 32.593035645137945 35.5 31.5 39.0 21.5 40.0 68-69 32.13580690040978 35.0 31.0 38.0 20.0 40.0 70-71 31.614743019194034 35.0 31.0 37.5 18.5 39.5 72-73 31.05636261629961 34.5 30.0 37.0 17.0 39.0 74-75 30.526122494844376 34.0 29.5 36.0 13.0 39.0 76-77 29.136230910453442 32.5 28.0 35.0 11.5 37.0 78-79 29.924343511999606 34.0 29.0 35.0 9.5 37.0 80-81 29.758806999843976 34.0 29.0 35.0 7.5 37.0 82-83 29.351070615255146 34.0 29.0 35.0 6.5 36.5 84-85 28.89061874746867 33.5 29.0 35.0 2.0 36.0 86-87 28.474415904944145 33.0 28.5 35.0 2.0 36.0 88-89 27.973449048867458 33.0 27.0 35.0 2.0 35.0 90-91 27.392765879358027 32.5 26.0 34.5 2.0 35.0 92-93 26.804161927374235 32.0 25.5 34.0 2.0 35.0 94-95 26.060478221890236 32.0 24.5 34.0 2.0 35.0 96-97 25.230121042943445 31.5 21.5 34.0 2.0 35.0 98-99 24.0712280284786 31.0 10.0 34.0 2.0 35.0 100-101 20.264734247966246 27.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2879765636232534 1101 2 0.3556057339446639 1101 3 0.2839792815374551 1101 4 0.1944869846255699 1101 5 0.2414314476299353 1101 6 0.24026665139372483 1101 7 0.27721765704074386 1101 8 0.3114199314067392 1101 9 0.5147613498124031 1101 10-11 0.45957861383217136 1101 12-13 0.33951811164283185 1101 14-15 0.4782422937776687 1101 16-17 0.44745293732078295 1101 18-19 0.3960049435623745 1101 20-21 0.3085467375864752 1101 22-23 0.30390329557242524 1101 24-25 0.40777978882263 1101 26-27 0.36146500304393925 1101 28-29 0.470902007881314 1101 30-31 0.44511102246276124 1101 32-33 0.33432085697639735 1101 34-35 0.4114168201034616 1101 36-37 0.4199026122114162 1101 38-39 0.43982554668837537 1101 40-41 0.3973390996352961 1101 42-43 0.44209883802214733 1101 44-45 0.47046322112964845 1101 46-47 0.3899237786200942 1101 48-49 0.6767234480284614 1101 50-51 0.8669189231180496 1101 52-53 0.653411467012063 1101 54-55 0.5270568737989763 1101 56-57 0.6572167317552626 1101 58-59 0.630027879283162 1101 60-61 0.6463110052462184 1101 62-63 0.47688052582240914 1101 64-65 0.5201026842556544 1101 66-67 0.5628477089786443 1101 68-69 0.36601280593127683 1101 70-71 0.3466250619799425 1101 72-73 0.35089264610360615 1101 74-75 0.5162493609636769 1101 76-77 0.48675112405574694 1101 78-79 0.4992223796433528 1101 80-81 0.6221317033933822 1101 82-83 0.4902279707924002 1101 84-85 0.44110118170709 1101 86-87 0.2129106614509162 1101 88-89 0.26356298617831797 1101 90-91 0.1709302248816229 1101 92-93 0.3549022303328684 1101 94-95 0.24333389146726958 1101 96-97 0.34238351037055637 1101 98-99 -0.14899465916699128 1101 100-101 -0.17863113855771573 1102 1 0.31382960813878924 1102 2 0.435276794042899 1102 3 0.34328029019865625 1102 4 0.24170651943029497 1102 5 0.1722497814698869 1102 6 0.17954340583643358 1102 7 0.320608184085863 1102 8 0.158090571659379 1102 9 0.3332979696346712 1102 10-11 0.36284428244621125 1102 12-13 0.41597981323181443 1102 14-15 0.5421504928305154 1102 16-17 0.4643927946367654 1102 18-19 0.4128348791093259 1102 20-21 0.34593422650500116 1102 22-23 0.3645112621625515 1102 24-25 0.4588616650642052 1102 26-27 0.4005660017050374 1102 28-29 0.36959481185468945 1102 30-31 0.5263876546819759 1102 32-33 0.4115694714462279 1102 34-35 0.5376411382908657 1102 36-37 0.5004395542592377 1102 38-39 0.5577069216549475 1102 40-41 0.5511117179242149 1102 42-43 0.5935644880645654 1102 44-45 0.5231502726627184 1102 46-47 0.43050376079451524 1102 48-49 0.6497449950761691 1102 50-51 0.679686435798736 1102 52-53 0.7859983508449915 1102 54-55 0.8368576892042157 1102 56-57 0.8252297747469726 1102 58-59 0.7299519261185665 1102 60-61 0.6439633619768799 1102 62-63 0.611720409091042 1102 64-65 0.5141190907741375 1102 66-67 0.6555335858964284 1102 68-69 0.6986592935537672 1102 70-71 0.3888195449388938 1102 72-73 0.2814877633548143 1102 74-75 0.36777659894311654 1102 76-77 0.3503306689240695 1102 78-79 0.22934995238815148 1102 80-81 0.4670380397285996 1102 82-83 0.4520954345043293 1102 84-85 0.2373046345998553 1102 86-87 0.5585152192757157 1102 88-89 0.27902703461691303 1102 90-91 0.2521177688800904 1102 92-93 0.42076957658326464 1102 94-95 0.5102900187923929 1102 96-97 0.3994485832641672 1102 98-99 0.5753153932383164 1102 100-101 0.330701100584438 1103 1 0.14085061063723003 1103 2 0.2949434086587672 1103 3 0.30547149881827096 1103 4 0.20179162249088023 1103 5 0.17907598384340417 1103 6 0.24578364631113203 1103 7 0.12939761256806293 1103 8 0.14425585897980397 1103 9 0.1680809177920679 1103 10-11 0.35227159350180415 1103 12-13 0.298494842900773 1103 14-15 0.31636133761502094 1103 16-17 0.26104410631696595 1103 18-19 0.18486056617990698 1103 20-21 0.2104284879041316 1103 22-23 0.17583309226873212 1103 24-25 0.14454678374003294 1103 26-27 0.1782732940223397 1103 28-29 0.3966655879321408 1103 30-31 0.4597341959062149 1103 32-33 0.5272155520208699 1103 34-35 0.33857453929024217 1103 36-37 0.5656070277133196 1103 38-39 0.2824859660022341 1103 40-41 0.3698029655819326 1103 42-43 0.6096198831301578 1103 44-45 0.5543093138869537 1103 46-47 0.5298006845921464 1103 48-49 0.7101839785240145 1103 50-51 0.7090198602209696 1103 52-53 0.6293040972534882 1103 54-55 0.6587297613466276 1103 56-57 0.5600109661898216 1103 58-59 0.518822749041739 1103 60-61 0.2884933432385921 1103 62-63 0.6375829559367645 1103 64-65 0.6253556226292218 1103 66-67 0.4540778551031721 1103 68-69 0.5147568497686308 1103 70-71 0.30502523669404624 1103 72-73 0.7297804319994121 1103 74-75 0.6445288241148823 1103 76-77 0.6874539512476154 1103 78-79 0.5597036375849065 1103 80-81 0.7143092764556442 1103 82-83 0.4803598005255658 1103 84-85 0.564510092126401 1103 86-87 0.5953443495068207 1103 88-89 0.6144242472964869 1103 90-91 0.8980601486427418 1103 92-93 0.7811474654027535 1103 94-95 0.8380749969310557 1103 96-97 1.072566166024945 1103 98-99 0.9290870593844751 1103 100-101 0.9622360787230981 1104 1 0.08677712939839921 1104 2 0.08304719067186994 1104 3 0.07737032252285303 1104 4 0.10256365941762624 1104 5 0.06867023637467184 1104 6 0.059363530170578827 1104 7 0.06395501280629645 1104 8 0.12150459334549879 1104 9 0.07962796960863017 1104 10-11 0.11386964293598822 1104 12-13 -0.01723978336485743 1104 14-15 0.08085601450603264 1104 16-17 0.025986780527809117 1104 18-19 0.0547016174008661 1104 20-21 0.13933278361586332 1104 22-23 -0.016944602787845042 1104 24-25 -0.026547989934776695 1104 26-27 0.09450877645235778 1104 28-29 0.21686529758074613 1104 30-31 0.12874123134397308 1104 32-33 0.15389772891187192 1104 34-35 0.12371115334622829 1104 36-37 0.253029972918803 1104 38-39 0.28288575754481116 1104 40-41 0.24160146915549063 1104 42-43 0.22178765412478896 1104 44-45 0.47839247350577807 1104 46-47 0.5634661848899398 1104 48-49 0.46464366434890536 1104 50-51 0.4403705525973862 1104 52-53 0.6367683616966104 1104 54-55 0.4472599504472825 1104 56-57 0.6056391302755912 1104 58-59 0.6575405498160052 1104 60-61 0.3983757136971988 1104 62-63 0.3864995357461751 1104 64-65 0.3697955794487626 1104 66-67 0.4254413899453411 1104 68-69 0.360377568946717 1104 70-71 0.13332798386498368 1104 72-73 0.21303925421501546 1104 74-75 0.31168505550261827 1104 76-77 0.3461700769176552 1104 78-79 0.275722845446726 1104 80-81 0.23925343964801726 1104 82-83 0.3199376742599327 1104 84-85 0.1895136284575507 1104 86-87 0.15573055409288727 1104 88-89 0.23282563949422652 1104 90-91 0.13025416293696424 1104 92-93 -0.03882573292958824 1104 94-95 -0.014763746368526398 1104 96-97 0.4250147961619284 1104 98-99 0.4316561109032335 1104 100-101 0.2758677046909348 1105 1 -0.008786052960068957 1105 2 0.13443926501235381 1105 3 0.09455951293735865 1105 4 0.04169877411764844 1105 5 0.01030142890171959 1105 6 0.13377129210610406 1105 7 0.05739983785964853 1105 8 0.07570575155193637 1105 9 0.16983813080619115 1105 10-11 0.06551860086828043 1105 12-13 0.08069845708125456 1105 14-15 0.11081479832711949 1105 16-17 0.08687360294066337 1105 18-19 0.16743845138555002 1105 20-21 0.050450548984549926 1105 22-23 0.05928229423312814 1105 24-25 0.16316288257367262 1105 26-27 0.16527644012424503 1105 28-29 0.13431502777253712 1105 30-31 0.1393863981775425 1105 32-33 0.014427415127073573 1105 34-35 -0.28606442633284246 1105 36-37 -0.0612564621577647 1105 38-39 -0.11055654474055387 1105 40-41 0.02817255582134237 1105 42-43 -0.07726528415039269 1105 44-45 -0.11553913608234723 1105 46-47 -0.03941777765463428 1105 48-49 -0.018251603121164806 1105 50-51 -0.10063119103747198 1105 52-53 0.007239947468342223 1105 54-55 -0.0755848104765775 1105 56-57 -0.006383102257075279 1105 58-59 0.05781388624247086 1105 60-61 -0.07791703122426696 1105 62-63 -0.08265779759300784 1105 64-65 -0.2375056035831733 1105 66-67 -0.1337747385125425 1105 68-69 -0.1768290541601587 1105 70-71 -0.18485448245880676 1105 72-73 -0.12306199433576381 1105 74-75 0.025235354194688142 1105 76-77 -0.13194253432635605 1105 78-79 0.00722282113309447 1105 80-81 0.15165964477976956 1105 82-83 0.1787721700774405 1105 84-85 -0.021629986142475133 1105 86-87 -0.05501764404750631 1105 88-89 -0.01918974134685314 1105 90-91 0.20563611549356864 1105 92-93 0.08932119161515217 1105 94-95 -0.13045093922609752 1105 96-97 -0.12490237295602569 1105 98-99 -0.13768070458115034 1105 100-101 -0.3904572914426474 1106 1 0.09222056013217639 1106 2 0.19533482280017722 1106 3 0.18078084225261648 1106 4 0.019051723500986384 1106 5 0.03820477172219228 1106 6 0.06321420186669258 1106 7 0.18113372367917435 1106 8 0.1029742933232356 1106 9 0.18563142284257594 1106 10-11 0.20465164400685154 1106 12-13 0.08700810047653107 1106 14-15 0.16212206993825617 1106 16-17 0.21118299520585282 1106 18-19 0.13448809143243068 1106 20-21 0.019068008106152945 1106 22-23 -0.015884079448440502 1106 24-25 0.21589148070972897 1106 26-27 0.09320384957789685 1106 28-29 0.21449702732608245 1106 30-31 0.1157348272193488 1106 32-33 0.20296681464713373 1106 34-35 0.1748813574720529 1106 36-37 0.1892370782183761 1106 38-39 0.31432940034566315 1106 40-41 0.1245977888142562 1106 42-43 0.24173793868570925 1106 44-45 0.28863380883645107 1106 46-47 0.25523629065275344 1106 48-49 0.23682854923108465 1106 50-51 0.1773942289078363 1106 52-53 0.027738440687826937 1106 54-55 0.21875185730622349 1106 56-57 0.13207851669487525 1106 58-59 0.12438777429426295 1106 60-61 -0.052554636559385415 1106 62-63 0.1839813145226188 1106 64-65 0.1616150923261941 1106 66-67 -0.028239064088744215 1106 68-69 0.09092793562722079 1106 70-71 -0.20726958653827765 1106 72-73 0.09623560876708837 1106 74-75 0.027284505933064196 1106 76-77 -0.07312054370187937 1106 78-79 -0.15567515029388446 1106 80-81 -0.08644829930193154 1106 82-83 -0.005107371191602539 1106 84-85 -0.016174882621072584 1106 86-87 0.09024018284015867 1106 88-89 0.00870139376113599 1106 90-91 0.24336317894577064 1106 92-93 0.0680414047966913 1106 94-95 0.10041085551691253 1106 96-97 0.3175661660249425 1106 98-99 0.22341471089962184 1106 100-101 -0.04508715360013227 1107 1 -0.04138749585300516 1107 2 0.09219687431723855 1107 3 -0.10972344447095139 1107 4 0.02740677687021531 1107 5 -0.058432680302786366 1107 6 0.06850458203892629 1107 7 -0.0335926993412059 1107 8 -0.08129037605485223 1107 9 -0.04090616475361486 1107 10-11 -0.0258313523079039 1107 12-13 0.03244904107341995 1107 14-15 -0.027589316134182695 1107 16-17 -0.07531143873659829 1107 18-19 -0.06834004818682615 1107 20-21 -0.11867103162958159 1107 22-23 -0.021998508235355985 1107 24-25 0.04572156319812848 1107 26-27 0.048104343171679886 1107 28-29 0.012244707339831962 1107 30-31 0.16026448103539082 1107 32-33 0.10310835290303544 1107 34-35 0.12264944477228568 1107 36-37 0.12945745304413947 1107 38-39 0.14973628106208992 1107 40-41 0.13755402890896562 1107 42-43 0.16863748709990745 1107 44-45 0.23673724394366502 1107 46-47 0.06172688181961661 1107 48-49 0.012903929689734639 1107 50-51 0.07418467591094924 1107 52-53 -0.04730428333882486 1107 54-55 0.03309865706180659 1107 56-57 0.21187041010917085 1107 58-59 0.06341533725849757 1107 60-61 -0.0028724412604574923 1107 62-63 -0.08971560266436995 1107 64-65 -0.10894435374128619 1107 66-67 0.20335439889649365 1107 68-69 0.07426437182282086 1107 70-71 -0.11999740823349114 1107 72-73 -0.04254766346372563 1107 74-75 0.07776389815395035 1107 76-77 0.08288361413356427 1107 78-79 0.1659741164758941 1107 80-81 0.11598303196919346 1107 82-83 0.376242755787068 1107 84-85 0.13610882401045998 1107 86-87 0.31684698813316103 1107 88-89 0.33063230495244156 1107 90-91 0.15609338745811385 1107 92-93 0.1252211894103148 1107 94-95 0.22036154387497575 1107 96-97 0.2868675207823479 1107 98-99 0.4332141466560522 1107 100-101 0.07920992875460442 1108 1 -0.007886277474661085 1108 2 0.15750459746995205 1108 3 -0.013003151531378876 1108 4 -0.01963320268394142 1108 5 -0.09582786231044338 1108 6 0.011242562395054279 1108 7 0.023956179001629607 1108 8 0.04747701282595784 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-1.94477002569338 2316 96-97 -2.0542946937035644 2316 98-99 -1.3294861872218604 2316 100-101 -0.948793333041607 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 4.0 4 18.0 5 36.0 6 96.0 7 222.0 8 452.0 9 878.0 10 1423.0 11 2238.0 12 3265.0 13 4577.0 14 6227.0 15 8178.0 16 10234.0 17 13189.0 18 11730.0 19 8324.0 20 7549.0 21 8172.0 22 8512.0 23 9587.0 24 11099.0 25 13455.0 26 16370.0 27 19862.0 28 25006.0 29 30702.0 30 37926.0 31 47135.0 32 59191.0 33 75871.0 34 100106.0 35 136904.0 36 192847.0 37 238761.0 38 122366.0 39 4485.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.80567599973158 19.443072238747085 15.298049814974569 22.453201946546773 2 15.611978092877274 20.827128093928323 39.46419071550655 24.09670309768785 3 20.064061416174457 24.218675471203895 30.136999590925335 25.580263521696317 4 13.05672213711454 17.107668179742035 37.488267939644054 32.34734174349937 5 13.6595552372044 37.40570396522355 34.17229283168234 14.762447965889717 6 31.502127325891127 36.57343296154645 17.19217234613771 14.73226736642471 7 28.32798165879275 33.03349404525156 20.49101209704801 18.147512198907677 8 26.47078558580408 33.99292629196224 19.584146809757673 19.952141312475998 9 25.849516329048228 17.22403754896936 20.691545176305866 36.23490094567655 10-11 25.06384402897658 26.518897751736258 27.120555473367396 21.296702745919763 12-13 26.394281644528412 23.860690267332473 27.687168704259257 22.05785938387986 14-15 22.827646224495115 25.471435379409833 26.708345678453444 24.992572717641604 16-17 21.850948789920967 28.74697641930435 27.290564487963888 22.1115103028108 18-19 21.65563103966641 27.260053428576164 29.845749137919537 21.23856639383789 20-21 22.411961626174527 25.861183890883428 30.034301032588 21.692553450354048 22-23 21.958163954517214 25.94281870871802 30.058597626264604 22.04041971050016 24-25 22.31517601735497 26.168669302895044 30.015614459078034 21.500540220671958 26-27 22.017892503507483 26.10892414178352 30.214749654102246 21.65843370060675 28-29 21.45298537265218 26.941797804038487 30.067833577742242 21.537383245567092 30-31 21.792272958588708 26.20333089866681 29.942032840107892 22.062363302636594 32-33 21.712397677696515 26.311045250802067 29.9295087195519 22.047048351949513 34-35 22.56474162364571 26.143699969442054 29.64420199289568 21.64735641401656 36-37 22.33768165559881 25.8947825387503 30.03765994969242 21.729875855958472 38-39 21.970714875879384 26.421812825500307 29.936973601025755 21.67049869759455 40-41 22.15539382004351 26.37233336486125 29.728035350069 21.744237465026245 42-43 21.906908442412266 26.610031718211964 29.1811592123277 22.30190062704807 44-45 22.232844252825707 26.350158899289944 29.0364502278594 22.380546620024948 46-47 22.594925299050363 26.835025735671575 28.59391963938532 21.976129325892746 48-49 22.616073767231594 26.906631678000796 28.430918403973575 22.046376150794035 50-51 22.146536587258726 27.068290072337 28.675904697539504 22.109268642864766 52-53 22.73202031054339 27.292029946814573 27.795468527307754 22.180481215334282 54-55 22.118669553224823 26.894546312928558 28.356432265856533 22.630351867990086 56-57 22.72133950750785 26.976709402141285 27.992658903807786 22.309292186543075 58-59 23.010995824876844 26.765292111082772 27.867784937300392 22.35592712673999 60-61 22.472846627403584 26.98315621829412 28.730592462943278 21.813404691359022 62-63 22.328429252455745 26.961625614290092 28.614337787399645 22.095607345854518 64-65 22.014513011344757 27.22293139869632 28.363874631252934 22.398680958705988 66-67 22.220852403566898 27.09839937363807 28.03584038441872 22.64490783837631 68-69 22.267206150438366 26.855863248139627 28.201675848939157 22.67525475248285 70-71 22.555805006059305 26.928810670840402 28.00190146498499 22.513482858115303 72-73 22.447278600843674 26.670326134583267 28.441543637942267 22.440851626630796 74-75 22.13573089082568 27.541028137911294 27.78303947974231 22.54020149152071 76-77 22.28660816420571 27.100045675574076 27.819375335998416 22.793970824221798 78-79 22.29099902015632 27.074123719007996 27.966250367845607 22.668626892990076 80-81 22.517630804664794 27.251138569818067 27.672774205648732 22.55845641986841 82-83 22.447761169897564 27.166980742350415 27.92407695298889 22.461181134763127 84-85 22.311597925747115 27.38550601851047 27.694404618361478 22.608491437380934 86-87 22.335795219370862 27.32212496119619 27.493427605028977 22.848652214403973 88-89 22.09899528524586 27.360125726777085 27.24908809282051 23.291790895156545 90-91 22.25111598976964 27.036724279482726 27.622257624161566 23.08990210658607 92-93 22.52988759612213 26.954537223274976 27.73010296143473 22.78547221916816 94-95 22.206745629625377 26.925525477700567 27.749135302651144 23.118593590022908 96-97 21.864456585840646 26.877397183585916 28.13932272351179 23.118823507061652 98-99 21.921587268480906 26.97432402608584 27.43285531931062 23.671233386122637 100-101 21.469630611712827 26.877085476494116 27.72726262199595 23.926021289797106 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 152.0 1 130.0 2 126.5 3 147.5 4 132.0 5 126.5 6 219.5 7 420.0 8 682.5 9 893.5 10 901.0 11 855.0 12 924.5 13 1025.5 14 1177.5 15 1326.0 16 1464.0 17 1610.5 18 1739.0 19 1922.5 20 2139.5 21 2436.5 22 2894.5 23 3503.0 24 4151.5 25 4980.5 26 6184.0 27 7929.0 28 9801.0 29 12015.5 30 14676.0 31 17513.0 32 20850.0 33 23156.0 34 24926.5 35 27089.5 36 29684.5 37 32423.5 38 34487.5 39 35578.5 40 36679.0 41 38821.0 42 40807.0 43 42681.0 44 44134.0 45 45604.5 46 46620.0 47 50399.5 48 58931.0 49 57817.0 50 48708.5 51 43993.5 52 41502.5 53 40267.0 54 38697.5 55 35435.5 56 31736.5 57 28329.5 58 25563.5 59 22100.5 60 18292.5 61 15082.0 62 12426.0 63 9589.0 64 6849.5 65 5296.0 66 4190.5 67 3403.5 68 2535.0 69 1792.5 70 1351.5 71 1001.0 72 849.0 73 790.5 74 642.5 75 530.0 76 424.5 77 275.5 78 177.5 79 114.5 80 76.5 81 51.0 82 45.0 83 30.0 84 16.5 85 8.5 86 3.0 87 3.0 88 1.5 89 0.5 90 0.5 91 1.0 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01147939489037582 2 0.011398554081288667 3 0.004931289354316373 4 3.2336323634861463E-4 5 0.005658856636100757 6 0.0 7 2.42522427261461E-4 8 0.001940179418091688 9 8.084080908715366E-5 10-11 0.0 12-13 0.0 14-15 4.446244499793452E-4 16-17 0.004446244499793452 18-19 0.0016572365862866502 20-21 8.084080908715367E-4 22-23 2.8294283180503783E-4 24-25 3.637836408921915E-4 26-27 4.042040454357683E-5 28-29 8.084080908715366E-5 30-31 4.446244499793452E-4 32-33 0.0030315303407682623 34-35 4.0420404543576834E-4 36-37 6.871468772408062E-4 38-39 0.00460792611796776 40-41 0.0 42-43 0.001010510113589421 44-45 0.004203722072531991 46-47 0.0 48-49 0.002182701845353149 50-51 8.892488999586904E-4 52-53 4.85044854522922E-4 54-55 8.084080908715366E-5 56-57 0.01022636234952494 58-59 0.0023848038680710335 60-61 0.0035569955998347616 62-63 1.6168161817430732E-4 64-65 0.0032336323634861467 66-67 0.0011721917317637283 68-69 0.0011317713272201513 70-71 0.004688766927054913 72-73 0.002021020227178842 74-75 0.005941799467905795 76-77 0.0011317713272201513 78-79 0.005416334208839296 80-81 0.0024656446771581866 82-83 0.002910269127137532 84-85 0.0018997590135481112 86-87 5.658856636100757E-4 88-89 0.0025060650817017634 90-91 0.007477774840561715 92-93 0.0025060650817017634 94-95 4.446244499793452E-4 96-97 4.042040454357683E-5 98-99 4.85044854522922E-4 100-101 0.0017380773953738038 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1236999.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.89072451004031 #Duplication Level Percentage of deduplicated Percentage of total 1 82.62013823320262 52.78660490838997 2 10.683647005975736 13.651718952426254 3 3.0175611841622114 5.783825109284965 4 1.2303147844115947 3.144228118058833 5 0.6179950706176712 1.9742076402698268 6 0.39388603788230586 1.509939860081382 7 0.2562352651952737 1.1459739716843844 8 0.17416682527937746 0.8902115722170423 9 0.13333521885180136 0.7666995361631737 >10 0.7877913888506387 9.270988862738974 >50 0.05387456160879501 2.381573060505719 >100 0.02739354936384654 3.2899554290770934 >500 0.0018935558266773569 0.8185551027083824 >1k 0.0016410817164537095 2.153294327727697 >5k 1.262370551118238E-4 0.4322235486662943 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5359 0.43322589589805655 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4566 0.36911913429194365 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4068 0.32886041136654115 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2831 0.22886033052573204 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2363 0.19102683187294411 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1973 0.1594989163289542 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1970 0.15925639390169274 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1506 0.12174625848525343 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1479 0.11956355663990029 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1453 0.11746169560363429 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1238 0.10008092164989625 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 8.084080908715367E-5 10-11 0.0 0.0 0.0 0.0 8.084080908715367E-5 12-13 0.0 0.0 0.0 0.0 8.084080908715367E-5 14-15 0.0 0.0 0.0 0.0 8.084080908715367E-5 16-17 0.0 0.0 0.0 4.0420404543576836E-5 8.084080908715367E-5 18-19 0.0 0.0 0.0 8.084080908715367E-5 8.084080908715367E-5 20-21 0.0 8.084080908715367E-5 0.0 8.084080908715367E-5 8.084080908715367E-5 22-23 0.0 8.084080908715367E-5 0.0 2.0210202271788417E-4 1.2126121363073051E-4 24-25 0.0 8.084080908715367E-5 0.0 2.42522427261461E-4 2.0210202271788417E-4 26-27 0.0 8.084080908715367E-5 0.0 3.6378364089219154E-4 2.42522427261461E-4 28-29 0.0 8.084080908715367E-5 0.0 6.467264726972293E-4 2.8294283180503783E-4 30-31 0.0 8.084080908715367E-5 0.0 0.001495554968112343 3.6378364089219154E-4 32-33 0.0 8.084080908715367E-5 0.0 0.0031123711498554163 4.0420404543576834E-4 34-35 0.0 8.084080908715367E-5 0.0 0.005214232186121411 4.0420404543576834E-4 36-37 0.0 8.084080908715367E-5 0.0 0.007599036054192445 4.0420404543576834E-4 38-39 0.0 8.084080908715367E-5 0.0 0.010266782754068515 4.0420404543576834E-4 40-41 0.0 8.084080908715367E-5 0.0 0.013581255926641816 4.0420404543576834E-4 42-43 0.0 8.084080908715367E-5 0.0 0.0204123042945063 4.0420404543576834E-4 44-45 0.0 8.084080908715367E-5 0.0 0.03181085837579497 4.0420404543576834E-4 46-47 0.0 8.084080908715367E-5 0.0 0.04434118378430379 4.4462444997934514E-4 48-49 0.0 1.6168161817430734E-4 0.0 0.06806796125138338 4.85044854522922E-4 50-51 0.0 1.6168161817430734E-4 0.0 0.09114801224576577 4.85044854522922E-4 52-53 0.0 1.6168161817430734E-4 0.0 0.10998392076307256 4.85044854522922E-4 54-55 0.0 1.6168161817430734E-4 0.0 0.14130973428434462 4.85044854522922E-4 56-57 0.0 1.6168161817430734E-4 0.0 0.1823768653006187 4.85044854522922E-4 58-59 0.0 1.6168161817430734E-4 0.0 0.23338741583461264 4.85044854522922E-4 60-61 0.0 1.6168161817430734E-4 0.0 0.28755075792300555 4.85044854522922E-4 62-63 0.0 1.6168161817430734E-4 0.0 0.36956375874192304 4.85044854522922E-4 64-65 0.0 1.6168161817430734E-4 0.0 0.4369445731160656 4.85044854522922E-4 66-67 0.0 1.6168161817430734E-4 0.0 0.5381976864977256 4.85044854522922E-4 68-69 0.0 1.6168161817430734E-4 0.0 0.673120996864185 4.85044854522922E-4 70-71 0.0 1.6168161817430734E-4 0.0 0.7571145975057376 5.658856636100757E-4 72-73 0.0 1.6168161817430734E-4 0.0 0.8718277056004087 6.467264726972294E-4 74-75 0.0 1.6168161817430734E-4 0.0 1.0182708312617876 6.467264726972294E-4 76-77 0.0 1.6168161817430734E-4 0.0 1.134964539179094 6.467264726972294E-4 78-79 0.0 1.6168161817430734E-4 0.0 1.2404213746332857 6.467264726972294E-4 80-81 0.0 1.6168161817430734E-4 0.0 1.400122392984958 6.871468772408062E-4 82-83 0.0 1.6168161817430734E-4 0.0 1.61236993724328 8.084080908715367E-4 84-85 0.0 1.6168161817430734E-4 0.0 1.8513757893094498 8.084080908715367E-4 86-87 0.0 1.6168161817430734E-4 0.0 2.1865822041893326 8.084080908715367E-4 88-89 0.0 1.6168161817430734E-4 0.0 2.559500856508372 8.084080908715367E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGTGCG 35 0.008395201 27.14167 36-37 CGAATAG 90 8.94178E-4 26.392 3 TAAGGTG 600 0.0 24.542576 5 GTAAGGT 410 0.0 23.17159 4 GTACATG 4645 0.0 23.11827 1 CGGTTAC 75 3.0360634E-5 22.165697 28-29 GAGTACT 5545 0.0 22.058084 12-13 TACATGG 4815 0.0 22.004225 2 GTCTACG 110 0.0028721504 21.597818 1 ACATGGG 4800 0.0 21.377522 3 ATTCGCG 100 5.392503E-7 21.374495 80-81 GTACTTT 5755 0.0 21.17065 14-15 AGTACTT 5750 0.0 20.40428 12-13 CATGGGG 2205 0.0 20.034756 4 AGAGTAC 8445 0.0 19.798632 10-11 GTATCAA 11505 0.0 19.658617 1 AGGCGAC 245 1.7555976E-7 19.388474 5 GGCCGAT 300 3.7180143E-9 18.999168 7 TACTTTT 6395 0.0 18.420578 14-15 GTATAGC 465 0.0 18.392982 1 >>END_MODULE