FastQCFastQC Report
Thu 26 May 2016
SRR936510_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936510_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1327168
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT49730.3747076481651155No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39340.2964206490813522No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32180.24247118676761345No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT30320.22845638231180984No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT21410.16132094806384722No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC19090.14384011669961905No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17780.13396947485171432No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT16210.12213977431643921No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTACG1004.9424085E-528.5095211
GTACATG54350.025.5283051
TACATGG55950.024.286892
ACATGGG55100.023.1094253
GAGTACT43650.022.6391712-13
CATGGGG23750.020.8053824
GTACTTT47050.020.69908114-15
AGTACTT44950.019.55345212-13
GTATCAA111600.019.4150871
GTTCGCG1250.0060239319.0041987
AGAGTAC76750.018.75658610-11
ATAGTAC3208.247298E-917.8171083
CATGGGA32450.017.570034
TCAACGC123200.017.5471524
CAACGCA123600.017.453245
ATCAACG123900.017.4480153
ACTTTTT55750.017.42597616-17
TATCAAC124950.017.3013942
GGACCGT1650.001460701817.2784986
TAGCCCT8800.017.2771954
Produced by FastQC (version 0.11.5)