##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936510_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1327168 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.43029593841925 31.0 31.0 34.0 30.0 34.0 2 31.481623276028355 31.0 31.0 34.0 28.0 34.0 3 31.524327741476586 31.0 31.0 34.0 28.0 34.0 4 35.165964670637024 37.0 35.0 37.0 32.0 37.0 5 35.103729897043934 37.0 35.0 37.0 32.0 37.0 6 35.21505340695376 37.0 35.0 37.0 32.0 37.0 7 35.05558301586536 37.0 35.0 37.0 32.0 37.0 8 35.044881281043544 37.0 35.0 37.0 32.0 37.0 9 36.567726919274726 39.0 37.0 39.0 32.0 39.0 10-11 36.447281730722864 39.0 37.0 39.0 32.0 39.0 12-13 36.35822593673144 39.0 37.0 39.0 32.0 39.0 14-15 37.44608444447124 40.0 37.0 41.0 32.0 41.0 16-17 37.39604895536963 40.0 37.0 41.0 32.0 41.0 18-19 37.36152092274678 40.0 37.0 41.0 32.0 41.0 20-21 37.26097336584366 39.5 37.0 41.0 31.0 41.0 22-23 37.16534606018229 39.0 36.0 41.0 31.0 41.0 24-25 37.023865855716835 39.0 36.0 41.0 31.0 41.0 26-27 36.81964755027246 39.0 36.0 41.0 30.5 41.0 28-29 36.93993563738728 39.0 36.0 41.0 30.5 41.0 30-31 36.89467686080436 39.0 36.0 41.0 30.0 41.0 32-33 36.75692301200752 39.0 36.0 41.0 30.0 41.0 34-35 36.58903130575783 39.0 35.0 41.0 30.0 41.0 36-37 36.36015221886001 39.0 35.0 40.5 30.0 41.0 38-39 36.181287900250766 39.0 35.0 40.0 30.0 41.0 40-41 36.05729116434392 39.0 35.0 40.0 29.5 41.0 42-43 35.80439477142306 38.0 35.0 40.0 28.5 41.0 44-45 35.54971073744997 38.0 35.0 40.0 27.5 41.0 46-47 35.24353887375223 38.0 34.0 40.0 26.5 41.0 48-49 35.01414741765926 38.0 34.0 40.0 26.0 41.0 50-51 34.31452498854704 37.5 33.5 39.5 25.0 40.5 52-53 34.51116814148623 38.0 33.5 39.5 25.5 40.5 54-55 34.88107797957757 38.0 34.0 40.0 26.0 41.0 56-57 34.801921459830254 38.0 34.0 40.0 25.5 41.0 58-59 34.58124442421759 38.0 34.0 40.0 25.0 41.0 60-61 34.2572044383228 38.0 34.0 40.0 23.5 41.0 62-63 33.96160433343782 37.0 33.0 40.0 23.0 41.0 64-65 33.658543605632445 37.0 33.0 40.0 23.0 41.0 66-67 33.283405718040214 36.0 32.5 39.0 22.0 41.0 68-69 32.9087982832618 36.0 32.0 39.0 21.5 41.0 70-71 32.50687780296089 35.0 32.0 38.5 20.5 40.0 72-73 32.09684757317838 35.0 31.5 38.0 20.0 40.0 74-75 31.672885798934274 35.0 31.0 37.0 18.5 39.0 76-77 31.255476699257365 35.0 31.0 36.5 17.5 39.0 78-79 30.80012929787337 35.0 30.5 36.0 14.0 39.0 80-81 30.38909731096591 34.0 30.0 36.0 9.5 37.5 82-83 29.98352846060182 34.0 30.0 35.0 7.0 37.0 84-85 29.59916529030236 34.0 29.0 35.0 6.0 36.5 86-87 29.28152426821623 34.0 29.0 35.0 2.0 36.0 88-89 28.912529536577132 34.0 29.0 35.0 2.0 36.0 90-91 28.57134251277909 34.0 29.0 35.0 2.0 35.5 92-93 28.216624421324205 34.0 29.0 35.0 2.0 35.0 94-95 27.80346760922506 34.0 27.0 35.0 2.0 35.0 96-97 27.240220152987412 33.5 26.5 35.0 2.0 35.0 98-99 26.599271908303997 33.0 25.0 35.0 2.0 35.0 100-101 24.53647428208034 31.0 20.0 34.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.32182591955847073 1101 2 0.43521748746225697 1101 3 0.3549308128859856 1101 4 0.34690588658614985 1101 5 0.3932528307057339 1101 6 0.2561211708378366 1101 7 0.40605934329311566 1101 8 0.2499378010594313 1101 9 0.4640008404256264 1101 10-11 0.4400107212094113 1101 12-13 0.5264329208884817 1101 14-15 0.6975827356062752 1101 16-17 0.6150211732625124 1101 18-19 0.6650011237221634 1101 20-21 0.7276367832685366 1101 22-23 0.6786783561878877 1101 24-25 0.6118193271202159 1101 26-27 0.6073435365208582 1101 28-29 0.6715798791021044 1101 30-31 0.5203492961002851 1101 32-33 0.421696330747217 1101 34-35 0.5962543850053308 1101 36-37 0.6427037781367275 1101 38-39 0.6332820135341279 1101 40-41 0.669244118305663 1101 42-43 0.544942495916942 1101 44-45 0.42768913960158983 1101 46-47 0.48554126609281667 1101 48-49 0.6064066834050976 1101 50-51 0.753612626006344 1101 52-53 0.7017188569523327 1101 54-55 0.7102738800469908 1101 56-57 0.5205897847241303 1101 58-59 0.6540679887025931 1101 60-61 0.7793447431557325 1101 62-63 0.7264676307073117 1101 64-65 0.669594693770037 1101 66-67 0.5820312372301757 1101 68-69 0.6059918566097906 1101 70-71 0.6249581898924319 1101 72-73 0.45506130948584556 1101 74-75 0.6295723105331454 1101 76-77 0.695798629762411 1101 78-79 0.7203682771685358 1101 80-81 0.6358771181174916 1101 82-83 0.6982861068288493 1101 84-85 0.6205346424735794 1101 86-87 0.540097413478005 1101 88-89 0.4947347315508388 1101 90-91 0.7618950369880757 1101 92-93 0.5814822085063582 1101 94-95 0.4346356936135791 1101 96-97 0.1997862975334499 1101 98-99 -0.0733374889730598 1101 100-101 0.45004295899740043 1102 1 0.3330517393413466 1102 2 0.30981137107931644 1102 3 0.3281867377345833 1102 4 0.36177840895830826 1102 5 0.4491975480137569 1102 6 0.5233823092353092 1102 7 0.20054021370145847 1102 8 0.24065990936773574 1102 9 0.536902307530994 1102 10-11 0.4927063087668344 1102 12-13 0.6097891032355278 1102 14-15 0.5971728441688953 1102 16-17 0.4621473633980955 1102 18-19 0.5027767026963019 1102 20-21 0.4077478096064979 1102 22-23 0.25085084041213435 1102 24-25 0.3800657547265942 1102 26-27 0.380331829878358 1102 28-29 0.3779585289836902 1102 30-31 0.3129766147784636 1102 32-33 0.348991166472743 1102 34-35 0.20532405264159337 1102 36-37 0.060211288508199345 1102 38-39 0.08419458327329465 1102 40-41 0.06425687401593194 1102 42-43 0.1712673413383925 1102 44-45 0.06606177323852336 1102 46-47 0.24905655705017438 1102 48-49 0.18414459130966776 1102 50-51 0.08857082224560031 1102 52-53 -0.005972518439072871 1102 54-55 0.03189560369711586 1102 56-57 0.20168908057713253 1102 58-59 0.232020876023185 1102 60-61 -0.043129149362513886 1102 62-63 0.12498641855719939 1102 64-65 0.18822677299054646 1102 66-67 0.0506402687498948 1102 68-69 0.1697119939422933 1102 70-71 0.22062502345624324 1102 72-73 0.05641595796867094 1102 74-75 -0.04832156672592802 1102 76-77 0.03420325201854624 1102 78-79 0.012474240959800653 1102 80-81 0.22627357830855033 1102 82-83 0.2059552292677722 1102 84-85 -0.04863369316452193 1102 86-87 -0.031509607328505496 1102 88-89 -0.1572211440233673 1102 90-91 -0.22031352880913246 1102 92-93 -0.18596390755868697 1102 94-95 -0.029413072142304486 1102 96-97 -0.29020662920665075 1102 98-99 -0.4716019573659196 1102 100-101 -0.14529743170086817 1103 1 0.2629407912986288 1103 2 0.44367475361007536 1103 3 0.3912134409531838 1103 4 0.2409758817058929 1103 5 0.35242030251583145 1103 6 0.24247700274364803 1103 7 0.38866152735582205 1103 8 0.24190138289066 1103 9 0.45886254345710853 1103 10-11 0.47115070552145966 1103 12-13 0.5460487834984065 1103 14-15 0.6706972675067533 1103 16-17 0.5567278007290568 1103 18-19 0.6162019235309089 1103 20-21 0.4436363169768711 1103 22-23 0.3533525941665516 1103 24-25 0.421762996717014 1103 26-27 0.5426700691089152 1103 28-29 0.4915602790459914 1103 30-31 0.3606770042965479 1103 32-33 0.42736552842919195 1103 34-35 0.48457713786483225 1103 36-37 0.6770597918172498 1103 38-39 0.597406007598785 1103 40-41 0.3720009689401991 1103 42-43 0.5410130007961982 1103 44-45 0.6254234758571542 1103 46-47 0.59853539200342 1103 48-49 0.5449899470910609 1103 50-51 0.13910779415783736 1103 52-53 0.5708517322924251 1103 54-55 0.5676716169518059 1103 56-57 0.7295368546935705 1103 58-59 0.5945565280044676 1103 60-61 0.8252238906323228 1103 62-63 0.7756621909014143 1103 64-65 0.7500327708306571 1103 66-67 0.7114226278265008 1103 68-69 0.5600342693925029 1103 70-71 0.670776284774881 1103 72-73 0.6402142669801982 1103 74-75 0.5659061121339484 1103 76-77 0.44437179704849683 1103 78-79 0.5721140059567098 1103 80-81 0.657185461379278 1103 82-83 0.7521291601698508 1103 84-85 0.48993563551674413 1103 86-87 0.5449442781605605 1103 88-89 0.4653385990054595 1103 90-91 0.7297604288607715 1103 92-93 0.40340660998347744 1103 94-95 0.27286034740190246 1103 96-97 0.40897718271703454 1103 98-99 0.30805668356671134 1103 100-101 0.4022731569144966 1104 1 0.2331228783959176 1104 2 0.2937539958184985 1104 3 0.3796626810568853 1104 4 0.31526721876966235 1104 5 0.3105401555443592 1104 6 0.3512964752796961 1104 7 0.32147314939359717 1104 8 0.19344846811814165 1104 9 0.5886425255268648 1104 10-11 0.5463807638539677 1104 12-13 0.4668854544012646 1104 14-15 0.6450267263945761 1104 16-17 0.32000215982976954 1104 18-19 0.4801285936430517 1104 20-21 0.449944562100967 1104 22-23 0.48804792324995105 1104 24-25 0.5123949595363939 1104 26-27 0.3502385117254505 1104 28-29 0.23693418805707012 1104 30-31 0.32460566071616626 1104 32-33 0.07886864750489053 1104 34-35 0.13313097233589843 1104 36-37 0.35438357101167384 1104 38-39 0.23891914606772247 1104 40-41 0.17669556843758727 1104 42-43 0.1839751887597174 1104 44-45 0.07277830638501825 1104 46-47 0.38385719100209315 1104 48-49 0.3282223034285039 1104 50-51 0.05496991080526925 1104 52-53 0.23917009001340972 1104 54-55 0.41315618620766514 1104 56-57 0.5545357182965134 1104 58-59 0.7158532582749899 1104 60-61 0.665291379248302 1104 62-63 0.44010342924188706 1104 64-65 0.3225582041608277 1104 66-67 0.44566022763367386 1104 68-69 0.5638964914670197 1104 70-71 0.6465971931820391 1104 72-73 0.29600284441353253 1104 74-75 0.2995109584185798 1104 76-77 0.2780895954711404 1104 78-79 0.34939775341605284 1104 80-81 0.6149311628380332 1104 82-83 0.28407585990089856 1104 84-85 0.4056270061185572 1104 86-87 0.3306402287290062 1104 88-89 0.41034252005279015 1104 90-91 0.26317871503649215 1104 92-93 0.2517296890045131 1104 94-95 0.31919287895458837 1104 96-97 0.34402454660054005 1104 98-99 0.4221794060455615 1104 100-101 0.6216806795956451 1105 1 0.14275638268573942 1105 2 0.1946009286744932 1105 3 0.13112029666570635 1105 4 0.2908328675078664 1105 5 0.3177624184053087 1105 6 0.33215667678955896 1105 7 0.3878899595888683 1105 8 0.16492868581482156 1105 9 0.33146417205259127 1105 10-11 0.42608938546693054 1105 12-13 0.31054730557406174 1105 14-15 0.3512872074380482 1105 16-17 0.2644019234036321 1105 18-19 0.2974886244050552 1105 20-21 -0.08299314994030027 1105 22-23 -0.13801093840751122 1105 24-25 -0.1535567045413444 1105 26-27 -0.052714898166030366 1105 28-29 0.08754076347305073 1105 30-31 0.08999340700479053 1105 32-33 -0.042797515198067515 1105 34-35 -0.30621735368963954 1105 36-37 -0.0997969350982757 1105 38-39 -0.29340187868543666 1105 40-41 -0.30830971829168874 1105 42-43 -0.2732111055657853 1105 44-45 -0.10483741196276952 1105 46-47 -0.24394963586114926 1105 48-49 -0.08231246791605429 1105 50-51 -0.14894449855381708 1105 52-53 -0.10719830054816981 1105 54-55 -0.09474183135687753 1105 56-57 0.06437533266061024 1105 58-59 -0.037304599197291566 1105 60-61 -0.006831851809742773 1105 62-63 -0.07669812106262697 1105 64-65 0.07051734672100451 1105 66-67 -0.281536718385361 1105 68-69 -0.13648619497379855 1105 70-71 -0.10802483207925917 1105 72-73 -0.17562641093412523 1105 74-75 -0.3675446013312431 1105 76-77 -0.2940659466366924 1105 78-79 -0.30187291969039975 1105 80-81 -0.11210872644381453 1105 82-83 -0.06529479830178886 1105 84-85 -0.3470385576955728 1105 86-87 -0.22566568728338865 1105 88-89 -0.2691551409166735 1105 90-91 -0.3164586345029612 1105 92-93 -0.3174713088045138 1105 94-95 -0.34096802694397965 1105 96-97 -0.36365863768862283 1105 98-99 -0.3677058662571824 1105 100-101 -0.013936346058780913 1106 1 -0.01811018267795106 1106 2 -0.019734589215058662 1106 3 -0.07957397944628397 1106 4 0.03125093450947247 1106 5 0.09293334188784286 1106 6 0.17624896636499443 1106 7 0.2910433688522218 1106 8 0.3272540949891507 1106 9 0.292754834035847 1106 10-11 0.4147711045959923 1106 12-13 0.27850959820477783 1106 14-15 0.12314184103758663 1106 16-17 0.1220499272561213 1106 18-19 0.058930836182234714 1106 20-21 0.1126208088276357 1106 22-23 0.24736845203453584 1106 24-25 -0.07859600834304104 1106 26-27 0.0690048783738888 1106 28-29 -0.2284201208978871 1106 30-31 -0.1039318540594607 1106 32-33 -0.20130686414096033 1106 34-35 -0.22834625397229757 1106 36-37 -0.06240804294952795 1106 38-39 -0.0414783698524559 1106 40-41 -0.12149070597548217 1106 42-43 -0.056335459354627915 1106 44-45 -0.37806165912045486 1106 46-47 0.13410356641230692 1106 48-49 0.1690264917118114 1106 50-51 -0.02594008964859995 1106 52-53 -0.0676102165300918 1106 54-55 0.12592883212366957 1106 56-57 0.19854505628963182 1106 58-59 0.2473587235268866 1106 60-61 0.22055880066371714 1106 62-63 0.20218648054756727 1106 64-65 0.11687903881796302 1106 66-67 0.1650983298819355 1106 68-69 0.283947128175285 1106 70-71 0.2786323112981819 1106 72-73 0.4096938973452744 1106 74-75 0.2790930773063067 1106 76-77 0.05043121762183844 1106 78-79 0.1526366477755623 1106 80-81 0.02213909894815913 1106 82-83 0.1791167777553646 1106 84-85 0.2767646744224628 1106 86-87 0.4033561994204966 1106 88-89 0.49824910854764326 1106 90-91 0.3548662829944682 1106 92-93 0.20480489221242948 1106 94-95 0.22185614089792338 1106 96-97 0.2250259141468689 1106 98-99 0.31579989121863505 1106 100-101 0.5503624478152886 1107 1 0.3967204828380879 1107 2 0.55911259990555 1107 3 0.5569613658640442 1107 4 0.5318030739483461 1107 5 0.4694929802513883 1107 6 0.5227799021344879 1107 7 0.577146281583893 1107 8 0.5721881277171406 1107 9 0.6444625166462927 1107 10-11 0.8019320366052014 1107 12-13 0.7048664788468884 1107 14-15 0.7525872939353064 1107 16-17 0.8089895706514127 1107 18-19 0.941344342903399 1107 20-21 1.0054952072499432 1107 22-23 0.9298650096169183 1107 24-25 1.017168397735091 1107 26-27 1.102939947208725 1107 28-29 0.9405372526389115 1107 30-31 0.8743028687751959 1107 32-33 0.7166848740567531 1107 34-35 0.792885820432609 1107 36-37 0.6309046043169602 1107 38-39 0.6876429953197487 1107 40-41 0.751745815843627 1107 42-43 0.9005722351588759 1107 44-45 0.8457379583586473 1107 46-47 0.7060321047983038 1107 48-49 0.7231488713489327 1107 50-51 0.6236910509075386 1107 52-53 0.6710906732232687 1107 54-55 0.7632560011172913 1107 56-57 0.691646153805408 1107 58-59 0.7891284372854557 1107 60-61 0.8784842063820548 1107 62-63 0.9460944158274103 1107 64-65 0.6007766400818468 1107 66-67 0.5693506962904351 1107 68-69 0.6716850878293315 1107 70-71 0.7023740366460132 1107 72-73 0.7639804850369778 1107 74-75 0.6967599162065703 1107 76-77 0.736050704005013 1107 78-79 0.793045084877388 1107 80-81 0.8043629683608273 1107 82-83 0.8373687059150221 1107 84-85 0.8282559378505994 1107 86-87 0.7586613098889607 1107 88-89 0.5728120094303613 1107 90-91 0.5940984818404118 1107 92-93 0.6300415330620908 1107 94-95 0.6109667392158045 1107 96-97 0.8674981786760334 1107 98-99 0.9844310128381011 1107 100-101 1.380994689404023 1108 1 0.13557532309053144 1108 2 0.15977355787090985 1108 3 -0.051241933918522165 1108 4 0.16411952747490233 1108 5 0.2688643768717469 1108 6 0.20030097594723628 1108 7 0.37419572311421234 1108 8 0.048834728977041664 1108 9 0.31939714946989284 1108 10-11 0.19759331233881738 1108 12-13 0.23797375805091292 1108 14-15 0.33296436608924296 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-2.113764204605772 2316 62-63 -1.7873209569031587 2316 64-65 -1.9062945328180696 2316 66-67 -2.886714354239057 2316 68-69 -2.2331398184244406 2316 70-71 -1.8977340739140516 2316 72-73 -1.81021476827026 2316 74-75 -2.036327963314413 2316 76-77 -2.6212976525495257 2316 78-79 -2.600620997135433 2316 80-81 -2.7814354415273357 2316 82-83 -2.6739492257299666 2316 84-85 -2.681601161020051 2316 86-87 -2.872089580837585 2316 88-89 -3.1384028975931813 2316 90-91 -2.955961587218386 2316 92-93 -3.310473260146395 2316 94-95 -3.193357958102119 2316 96-97 -3.5541103250133297 2316 98-99 -4.173235833440092 2316 100-101 -4.965421648280309 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2726.0 3 1022.0 4 1001.0 5 1188.0 6 1307.0 7 1544.0 8 1851.0 9 2432.0 10 3157.0 11 3869.0 12 4907.0 13 5703.0 14 6612.0 15 8174.0 16 9382.0 17 11494.0 18 10167.0 19 7872.0 20 7811.0 21 8545.0 22 9424.0 23 10690.0 24 12498.0 25 14261.0 26 17030.0 27 20019.0 28 23896.0 29 29299.0 30 35352.0 31 44147.0 32 55649.0 33 71980.0 34 95227.0 35 130331.0 36 183373.0 37 242433.0 38 199557.0 39 31230.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.28621488724354 18.685657227905576 14.457695133668683 22.570432751182203 2 15.388869966753976 20.224614114037177 40.1581371375867 24.228378781622155 3 19.952165620886174 24.240229858207385 29.57420017949195 26.23340434141449 4 12.781096308579395 16.49550585087339 37.76193257834141 32.961465262205806 5 13.817586718448476 37.33355847163257 34.139735502701654 14.709119307217291 6 31.606319575076053 36.14145534189564 17.65447410384098 14.597750979187321 7 28.652602113225406 32.666434024451554 20.549718982775683 18.131244879547356 8 26.262992442884503 34.22219441992902 19.78280547078787 19.73200766639861 9 26.05302483942657 17.4524836035368 20.75596682047653 35.7385247365601 10-11 25.01931701080471 26.46573708813306 27.254474196619654 21.260471704442573 12-13 26.441405839232836 23.809669187028 27.584595304295846 22.164329669443323 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28.841941310377724 22.039465504418338 40-41 22.453294592846245 26.765333844715798 28.75569418474495 22.025677377692997 42-43 22.165070215591534 27.009013591025965 28.199184350070166 22.62673184331233 44-45 22.43695449632353 26.803884847039516 28.272249730909653 22.486910925727297 46-47 22.80688006305293 27.18769473280493 27.878089634921437 22.12733556922071 48-49 22.73525970097072 27.211657063461026 27.74331825603676 22.309764979531497 50-51 22.249754078195412 27.368431363610508 28.137259592834113 22.24455496535997 52-53 22.81375797522148 27.58425431796904 27.381414790633364 22.220572916176113 54-55 22.265079886552357 27.21164887893593 27.748746921933915 22.7745243125778 56-57 22.717067912758033 27.37819357692295 27.414625398367086 22.490113111951935 58-59 23.085460808407248 27.079687493524613 27.38067295813819 22.454178739929947 60-61 22.56921643555277 27.238625048458758 28.204786237072483 21.987372278915984 62-63 22.384570580784583 27.391981180768155 28.04337555654849 22.18007268189877 64-65 22.116514145132182 27.694771066997703 27.663613724300845 22.525101063569267 66-67 22.2844068565435 27.53972637421344 27.30192195102103 22.87394481822203 68-69 22.321093185149966 27.26497623191114 27.718372730804024 22.695557852134872 70-71 22.53912120877593 27.369718735717974 27.413838770848226 22.67732128465787 72-73 22.446893601085353 27.118048923103238 27.921968076068275 22.513089399743127 74-75 22.191701239810783 27.959074338915773 27.384492042274427 22.46473237899902 76-77 22.446523782110596 27.459589551001706 27.27377532905544 22.82011133783226 78-79 22.47126308994 27.46838195584723 27.433748048309592 22.62660690590318 80-81 22.63697264805119 27.626802241935838 27.215539506318915 22.520685603694055 82-83 22.574374032919998 27.55743329667519 27.506383151869844 22.36180951853497 84-85 22.434996810641795 27.77028633391092 27.25369696161354 22.541019893833745 86-87 22.48408728014751 27.775165532223685 27.17659709302282 22.56415009460599 88-89 22.288507054097618 27.72004542231526 26.882618579897084 23.108828943690042 90-91 22.381079052550987 27.42012907154096 27.2561521488814 22.942639727026652 92-93 22.710637340659183 27.470543308335664 27.406149478839524 22.412669872165626 94-95 22.312189428210704 27.388791327782585 27.418706438945108 22.880312805061607 96-97 22.048893220753417 27.361350717891007 27.76353846855193 22.826217592803648 98-99 22.107579637155652 27.618977637347836 27.04190854091655 23.231534184579964 100-101 22.00491329000472 27.383789912001 27.369053057200105 23.242243740794176 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 107.0 1 84.0 2 96.0 3 125.5 4 122.5 5 130.5 6 192.0 7 416.0 8 682.5 9 845.0 10 869.5 11 828.0 12 863.0 13 943.0 14 1034.5 15 1156.0 16 1270.5 17 1386.5 18 1533.0 19 1665.5 20 1869.5 21 2216.0 22 2649.5 23 3217.0 24 3928.0 25 4998.5 26 6367.5 27 8077.5 28 10062.5 29 12271.5 30 14902.0 31 17976.0 32 21634.5 33 24313.0 34 26445.0 35 28741.0 36 31487.0 37 34571.0 38 36591.5 39 38032.5 40 39542.5 41 41987.5 42 44137.0 43 46076.5 44 48273.5 45 49964.5 46 50950.0 47 54504.5 48 62995.0 49 61788.0 50 52591.0 51 47858.5 52 45063.5 53 44049.0 54 42313.5 55 38552.5 56 34637.0 57 30805.5 58 27712.0 59 24210.0 60 19924.5 61 16522.0 62 13858.0 63 10536.0 64 7709.5 65 6206.0 66 4684.0 67 3603.0 68 2777.5 69 1980.5 70 1459.5 71 1139.5 72 916.5 73 795.0 74 639.0 75 524.5 76 408.5 77 252.5 78 191.5 79 129.5 80 73.5 81 57.5 82 37.0 83 20.5 84 14.5 85 8.5 86 6.0 87 5.0 88 6.5 89 7.0 90 3.5 91 2.5 92 2.0 93 1.5 94 1.5 95 0.5 96 0.5 97 1.0 98 0.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01454224333317259 2 0.00693205381684911 3 0.006706008583690987 4 0.03337934609634952 5 0.043400684766359644 6 0.06125825818585138 7 0.028707744611081644 8 0.02554311134686792 9 0.015446424265805081 10-11 0.04648996961952066 12-13 0.043136965327675164 14-15 0.02030639677870473 16-17 0.02313196219318127 18-19 0.028933789844239766 20-21 0.021624993972127117 22-23 0.015145030621594252 24-25 0.002599520181318416 26-27 0.0030139364421083088 28-29 0.0015069682210541544 30-31 0.006291592322901094 32-33 9.795293436852003E-4 34-35 0.0016576650431595697 36-37 0.0054250855957949555 38-39 0.006404614939480156 40-41 0.003503701113950909 42-43 4.144162607898924E-4 44-45 8.665067271061387E-4 46-47 0.002185103920528524 48-49 5.27438877368954E-4 50-51 0.0013939456044750928 52-53 7.911583160534312E-4 54-55 0.0027125427978974778 56-57 0.0023734749481602933 58-59 0.0020720813039494623 60-61 6.404614939480157E-4 62-63 0.0011302261657906158 64-65 0.002486497564739355 66-67 0.0020344070984231084 68-69 0.005538108212374018 70-71 0.008024605777113373 72-73 0.0033530042918454937 74-75 0.0022604523315812317 76-77 0.0027125427978974778 78-79 0.0326635361913488 80-81 0.021022206683705455 82-83 0.0031269590586873704 84-85 0.0074218184886917105 86-87 0.006404614939480156 88-89 0.005048343540531417 90-91 0.011452958480011573 92-93 0.01337434296185562 94-95 0.005990198678690264 96-97 0.003880443169214448 98-99 0.021888713410811594 100-101 0.04234580701162174 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1327168.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.26817726446096 #Duplication Level Percentage of deduplicated Percentage of total 1 83.17316707844654 54.28561012522677 2 10.484440636822882 13.686006600057473 3 2.853076723717746 5.586453520581522 4 1.140842326988597 2.9784279691476754 5 0.6031813409964379 1.9684273343385392 6 0.3564662763169557 1.395954246687443 7 0.252069242178995 1.1516469987019888 8 0.1775052053749681 0.9268352967822373 9 0.13255165801506213 0.7786264600822752 >10 0.7452919536333709 8.92828209049794 >50 0.05421047976222676 2.4016134251701247 >100 0.024188067277515532 3.112387939869262 >500 0.0016202364062410089 0.7298252223656307 >1k 0.0013887740624922934 2.0699027704911384 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4973 0.3747076481651155 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3934 0.2964206490813522 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3218 0.24247118676761345 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3032 0.22845638231180984 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2141 0.16132094806384722 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1909 0.14384011669961905 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1778 0.13396947485171432 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1621 0.12213977431643921 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.767420552635386E-5 0.0 12-13 0.0 0.0 0.0 7.534841105270772E-5 0.0 14-15 0.0 0.0 0.0 7.534841105270772E-5 0.0 16-17 0.0 0.0 0.0 7.534841105270772E-5 0.0 18-19 0.0 0.0 0.0 7.534841105270772E-5 0.0 20-21 0.0 0.0 0.0 2.2604523315812317E-4 0.0 22-23 0.0 0.0 0.0 2.2604523315812317E-4 0.0 24-25 0.0 0.0 0.0 2.2604523315812317E-4 7.534841105270772E-5 26-27 0.0 0.0 0.0 3.7674205526353865E-4 7.534841105270772E-5 28-29 0.0 0.0 0.0 7.534841105270772E-4 7.534841105270772E-5 30-31 0.0 0.0 0.0 0.0017706876597386313 7.534841105270772E-5 32-33 0.0 0.0 0.0 0.0030516106476346627 7.534841105270772E-5 34-35 0.0 0.0 0.0 0.004332533635530694 7.534841105270772E-5 36-37 0.0 0.0 0.0 0.006592985967111926 7.534841105270772E-5 38-39 0.0 0.0 0.0 0.008891112504219511 7.534841105270772E-5 40-41 0.0 0.0 0.0 0.012545510440275836 1.5069682210541545E-4 42-43 0.0 0.0 0.0 0.018799428557650575 1.5069682210541545E-4 44-45 0.0 0.0 0.0 0.027125427978974777 1.5069682210541545E-4 46-47 0.0 0.0 0.0 0.0370714182379322 1.5069682210541545E-4 48-49 0.0 0.0 0.0 0.057453163427689635 1.5069682210541545E-4 50-51 0.0 0.0 0.0 0.07644096301297199 1.5069682210541545E-4 52-53 0.0 0.0 0.0 0.09482597530983267 1.5069682210541545E-4 54-55 0.0 0.0 0.0 0.12492766552538939 1.5069682210541545E-4 56-57 0.0 0.0 0.0 0.16117025124174184 1.5069682210541545E-4 58-59 0.0 0.0 0.0 0.2063416236678401 1.5069682210541545E-4 60-61 0.0 0.0 0.0 0.25490367459131025 1.5069682210541545E-4 62-63 0.0 0.0 0.0 0.3271628007908569 1.5069682210541545E-4 64-65 0.0 0.0 0.0 0.38514340309591555 1.5069682210541545E-4 66-67 0.0 0.0 0.0 0.47322569561653083 1.5069682210541545E-4 68-69 0.0 0.0 0.0 0.5907692168587548 1.5069682210541545E-4 70-71 0.0 0.0 0.0 0.6637441529633024 1.5069682210541545E-4 72-73 0.0 0.0 0.0 0.7594366350002411 1.5069682210541545E-4 74-75 0.0 0.0 0.0 0.896231675266432 1.5069682210541545E-4 76-77 0.0 0.0 0.0 0.9961813425278487 1.5069682210541545E-4 78-79 0.0 0.0 0.0 1.0898770916718907 1.5069682210541545E-4 80-81 0.0 0.0 0.0 1.2407246105994116 1.8837102763176933E-4 82-83 0.0 0.0 0.0 1.433465846072238 4.1441626078989247E-4 84-85 0.0 0.0 0.0 1.653407857935092 4.5209046631624635E-4 86-87 0.0 0.0 0.0 1.9697958359454117 4.5209046631624635E-4 88-89 0.0 0.0 0.0 2.331166815354198 4.5209046631624635E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGTACG 100 4.9424085E-5 28.509521 1 GTACATG 5435 0.0 25.528305 1 TACATGG 5595 0.0 24.28689 2 ACATGGG 5510 0.0 23.109425 3 GAGTACT 4365 0.0 22.63917 12-13 CATGGGG 2375 0.0 20.805382 4 GTACTTT 4705 0.0 20.699081 14-15 AGTACTT 4495 0.0 19.553452 12-13 GTATCAA 11160 0.0 19.415087 1 GTTCGCG 125 0.00602393 19.004198 7 AGAGTAC 7675 0.0 18.756586 10-11 ATAGTAC 320 8.247298E-9 17.817108 3 CATGGGA 3245 0.0 17.57003 4 TCAACGC 12320 0.0 17.547152 4 CAACGCA 12360 0.0 17.45324 5 ATCAACG 12390 0.0 17.448015 3 ACTTTTT 5575 0.0 17.425976 16-17 TATCAAC 12495 0.0 17.301394 2 GGACCGT 165 0.0014607018 17.278498 6 TAGCCCT 880 0.0 17.277195 4 >>END_MODULE