FastQCFastQC Report
Thu 26 May 2016
SRR936510_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936510_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1327168
Sequences flagged as poor quality0
Sequence length101
%GC45

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT54840.4132106862130491No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48230.3634053865072093No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40850.307798259150311No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT26610.20050212181125524No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25120.18927520856440178No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT20290.15288192602594397No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC19740.14873776341804504No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC15920.11995467039591068No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT15790.11897514105222548No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT15150.11415284274485221No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG55750.024.9859541
TACATGG56050.024.9158672
GTAAGGT6900.024.778554
GTATAGG3950.024.320681
ACATGGG57150.023.7695713
TAAGGTG7650.023.5891285
GAGTACT57350.022.52294712-13
CATGGGA27950.021.409734
ATGGGAG12550.021.190155
GTACTTT60850.021.1884414-15
AGTACTT58350.021.11962712-13
AGAGTAC86800.020.07872210-11
AGGTAAG7400.019.8991372
GGTGTGC13650.019.482528
GGTAAGG7350.019.386773
GTGTAGC8200.019.3304921
GTATCAA123200.019.2211361
CATGGGG26550.018.4244774
TGTAGCG1559.6240017E-418.3875482
TACTTTT67300.018.27571914-15
Produced by FastQC (version 0.11.5)