FastQCFastQC Report
Thu 26 May 2016
SRR936509_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936509_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1351691
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT51960.38440738304834465No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38510.2849023926326357No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30860.22830661741477898No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT30040.22224014216266882No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT20870.15439919330675428No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC19260.14248818701907462No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17640.13050319932588142No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT15210.11252571778609165No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG57400.026.1604751
TACATGG59900.024.036442
ACATGGG57900.022.9791413
GTATTAG4550.022.9763851
TACACCG1252.250541E-422.8084415
GAGTACT44000.021.33483912-13
CCTATAC3850.020.981793
GTACTTT45600.020.32453314-15
CATGGGG25550.020.2724384
CATGGGA31500.019.6111564
CATTCCG1951.02924205E-519.4951159
GTGTAGC7800.019.4951151
GTATAGA4150.019.4657551
GTATAGG4700.019.2099461
AGAGTAC74900.019.03417210-11
AGTACTT45950.018.6709612-13
GTGTAGG5500.018.143751
GTCCTAC5000.018.0573481
GTATCAA117100.017.611781
CGTACAC1350.00938121617.5991043
Produced by FastQC (version 0.11.5)