##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936509_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1351691 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.439772107678458 31.0 31.0 34.0 30.0 34.0 2 31.48430965361166 31.0 31.0 34.0 28.0 34.0 3 31.536795021939184 33.0 31.0 34.0 28.0 34.0 4 35.157874099923724 37.0 35.0 37.0 33.0 37.0 5 35.13193991822095 37.0 35.0 37.0 32.0 37.0 6 35.26026510496852 37.0 35.0 37.0 32.0 37.0 7 35.12751878942746 37.0 35.0 37.0 32.0 37.0 8 35.08535382716908 37.0 35.0 37.0 32.0 37.0 9 36.57383751167981 39.0 37.0 39.0 32.0 39.0 10-11 36.49086440614016 39.0 37.0 39.0 32.0 39.0 12-13 36.386954192933146 39.0 37.0 39.0 32.0 39.0 14-15 37.457748109590135 40.0 37.0 41.0 32.0 41.0 16-17 37.33546720367303 40.0 37.0 41.0 32.0 41.0 18-19 37.29442786849953 39.5 37.0 41.0 32.0 41.0 20-21 37.20379842730328 39.5 36.5 41.0 31.0 41.0 22-23 37.079136429849726 39.0 36.0 41.0 31.0 41.0 24-25 37.22011169712604 39.0 37.0 41.0 31.0 41.0 26-27 37.19712900359623 39.0 36.5 41.0 31.0 41.0 28-29 37.113795238704704 39.0 36.0 41.0 31.0 41.0 30-31 36.9504535430065 39.0 36.0 41.0 30.5 41.0 32-33 36.78192020217638 39.0 36.0 41.0 30.0 41.0 34-35 36.56659510198707 39.0 35.0 41.0 30.0 41.0 36-37 36.379768379015616 39.0 35.0 40.0 30.0 41.0 38-39 36.18441492915171 39.0 35.0 40.0 30.0 41.0 40-41 36.02912499972257 39.0 35.0 40.0 29.0 41.0 42-43 35.80391931291989 38.0 35.0 40.0 28.5 41.0 44-45 35.558594382887804 38.0 35.0 40.0 27.5 41.0 46-47 35.30417861774622 38.0 34.5 40.0 26.5 41.0 48-49 35.22037470102265 38.0 34.5 40.0 26.0 41.0 50-51 34.301715776756666 37.5 33.5 39.5 25.0 40.5 52-53 34.46294197416421 38.0 33.5 39.5 25.5 40.5 54-55 34.89263374543442 38.0 34.0 40.0 26.0 41.0 56-57 34.80279516546311 38.0 34.0 40.0 25.5 41.0 58-59 34.54799506691988 38.0 34.0 40.0 25.0 41.0 60-61 34.228033626028434 38.0 34.0 40.0 23.5 41.0 62-63 33.91686302564713 37.0 33.0 40.0 23.0 41.0 64-65 33.5873842468434 37.0 33.0 39.5 22.5 41.0 66-67 33.20243051111534 36.0 32.5 39.0 22.0 41.0 68-69 32.79643646365923 36.0 32.0 39.0 21.5 40.5 70-71 32.34589895175746 35.0 31.5 38.5 20.0 40.0 72-73 31.954114142951312 35.0 31.0 37.5 19.0 40.0 74-75 31.53343811566401 35.0 31.0 37.0 18.0 39.0 76-77 31.101995204525295 35.0 31.0 36.5 15.5 39.0 78-79 30.658288025887572 34.5 30.0 36.0 12.0 38.5 80-81 30.177991123710967 34.0 30.0 35.5 7.5 37.0 82-83 29.703235798714353 34.0 29.0 35.0 7.0 37.0 84-85 29.378279133322632 34.0 29.0 35.0 2.0 36.5 86-87 29.08188668859969 34.0 29.0 35.0 2.0 36.0 88-89 28.679512551315355 34.0 29.0 35.0 2.0 36.0 90-91 28.270454933856925 34.0 28.5 35.0 2.0 35.0 92-93 27.91153932370638 34.0 27.0 35.0 2.0 35.0 94-95 27.464113099813492 33.5 27.0 35.0 2.0 35.0 96-97 26.899743728411302 33.0 25.5 35.0 2.0 35.0 98-99 26.208250258380062 33.0 24.5 35.0 2.0 35.0 100-101 23.736990924700983 31.0 11.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.43177940089618616 1101 2 0.5099050933945364 1101 3 0.4392884816571794 1101 4 0.5558835374315478 1101 5 0.5204045368023458 1101 6 0.434789952490533 1101 7 0.38856004502595454 1101 8 0.5237343005118191 1101 9 0.7371592979362802 1101 10-11 0.6060759927932935 1101 12-13 0.7473043602646356 1101 14-15 0.8208268337135181 1101 16-17 0.8251914918733405 1101 18-19 0.7727427716492556 1101 20-21 0.7285122271762461 1101 22-23 0.7624147095018472 1101 24-25 0.7733170370266507 1101 26-27 0.6342810364712363 1101 28-29 0.6976026121824646 1101 30-31 0.6850586835123238 1101 32-33 0.527528878141247 1101 34-35 0.5216100661191092 1101 36-37 0.4089228657580577 1101 38-39 0.451917854330695 1101 40-41 0.5162414455501576 1101 42-43 0.49769955435652946 1101 44-45 0.2897703779407408 1101 46-47 0.393098185409265 1101 48-49 0.3676412213042468 1101 50-51 0.3831464204488313 1101 52-53 0.5500362579251785 1101 54-55 0.578609257602686 1101 56-57 0.36606932885259624 1101 58-59 0.4343674464078404 1101 60-61 0.24136636713898696 1101 62-63 0.3820427869353864 1101 64-65 0.3915439263701259 1101 66-67 0.4296476149559538 1101 68-69 0.5002464839197316 1101 70-71 0.43699007833158277 1101 72-73 0.28891781496110625 1101 74-75 0.3117650208768481 1101 76-77 0.3294470008660113 1101 78-79 0.2900742400351639 1101 80-81 0.29545133141714075 1101 82-83 0.4202103463074067 1101 84-85 0.3890684590129432 1101 86-87 0.3509482766862462 1101 88-89 0.34683792541699887 1101 90-91 0.24010127846892715 1101 92-93 0.17256931755098393 1101 94-95 0.11291435535579453 1101 96-97 -0.15692333707833228 1101 98-99 0.150806491670469 1101 100-101 0.6521772619353641 1102 1 0.25499856093716033 1102 2 0.40263032631000684 1102 3 0.3017127536439119 1102 4 0.3614282425678823 1102 5 0.4945915520698918 1102 6 0.44350523467188907 1102 7 0.3864477578204557 1102 8 0.3422157404415813 1102 9 0.5124924036384471 1102 10-11 0.5267795682286405 1102 12-13 0.45302993462686203 1102 14-15 0.544026287398701 1102 16-17 0.4210213538312928 1102 18-19 0.470242405813444 1102 20-21 0.5173299433852137 1102 22-23 0.43345339054179277 1102 24-25 0.2689599812732766 1102 26-27 0.3508220832494473 1102 28-29 0.3391256598386718 1102 30-31 0.2880965841025329 1102 32-33 0.23500109900852806 1102 34-35 0.04377717991844321 1102 36-37 0.04531952933544403 1102 38-39 0.14461596661790566 1102 40-41 0.17752177333904484 1102 42-43 0.3178741799845426 1102 44-45 0.06218167495064364 1102 46-47 0.2780985268560272 1102 48-49 0.5564331826719879 1102 50-51 0.5330538395991198 1102 52-53 0.31285114500385447 1102 54-55 0.6455138964007929 1102 56-57 0.9099473835816312 1102 58-59 0.8574954889423552 1102 60-61 0.698415486694131 1102 62-63 0.8242789705361844 1102 64-65 0.6967481603099301 1102 66-67 0.7648942858500547 1102 68-69 0.5996318797540781 1102 70-71 0.5661307056180576 1102 72-73 0.5297749438816446 1102 74-75 0.42798510770187903 1102 76-77 0.45616979487605747 1102 78-79 0.47171611116340273 1102 80-81 0.5252750473334373 1102 82-83 0.629320536054248 1102 84-85 0.7605769707928545 1102 86-87 0.7211386088651643 1102 88-89 0.8774360425820156 1102 90-91 0.8873508760495348 1102 92-93 0.5609384702752429 1102 94-95 0.6717196819252287 1102 96-97 0.4937651171434112 1102 98-99 0.45635080658193417 1102 100-101 0.4021229231226222 1103 1 0.37075882521276426 1103 2 0.49760908987772723 1103 3 0.43793738790535386 1103 4 0.3777718008500912 1103 5 0.46398654971029174 1103 6 0.34389787270870187 1103 7 0.3598672953381481 1103 8 0.2563865757412458 1103 9 0.6314277505001726 1103 10-11 0.49745630253727313 1103 12-13 0.4899246963069004 1103 14-15 0.625537377535565 1103 16-17 0.7575706695462543 1103 18-19 0.6469012071348175 1103 20-21 0.8306201746263611 1103 22-23 0.914545415081669 1103 24-25 0.9556310525332989 1103 26-27 0.6118640841932859 1103 28-29 0.4072162686163949 1103 30-31 0.38844108622618023 1103 32-33 0.7182375312879046 1103 34-35 0.6454232393804489 1103 36-37 0.6699103740735239 1103 38-39 0.6008330501478696 1103 40-41 0.5470347511587761 1103 42-43 0.5891814003715155 1103 44-45 0.593009423888823 1103 46-47 0.5142721936800001 1103 48-49 0.6512392232571713 1103 50-51 0.8899495451205865 1103 52-53 0.9091739387745008 1103 54-55 0.8054582097561465 1103 56-57 0.8887175581922975 1103 58-59 0.8467064375029878 1103 60-61 0.6933583842873361 1103 62-63 0.7725347515649759 1103 64-65 0.8807273957983668 1103 66-67 0.6560231202058802 1103 68-69 0.5186266886261919 1103 70-71 0.6863156985392536 1103 72-73 0.6595635234002906 1103 74-75 0.47841833092038044 1103 76-77 0.5145058024267932 1103 78-79 0.3443163943158325 1103 80-81 0.32185173445000004 1103 82-83 0.5382908050490585 1103 84-85 0.665898348801349 1103 86-87 0.701710576774552 1103 88-89 0.8342326447528521 1103 90-91 0.9750497906318856 1103 92-93 0.8608846713134675 1103 94-95 0.7632288843792203 1103 96-97 0.9464003224289215 1103 98-99 1.0280752048830202 1103 100-101 1.4031686994322037 1104 1 0.23518048120667245 1104 2 0.36923522992220725 1104 3 0.30169778078222365 1104 4 0.35718979974549825 1104 5 0.3772147974376665 1104 6 0.39620109841884954 1104 7 0.3622145554996621 1104 8 0.19730950798790303 1104 9 0.45439358275130104 1104 10-11 0.43418327401190737 1104 12-13 0.3479971814919196 1104 14-15 0.367858217026253 1104 16-17 0.18317856887902195 1104 18-19 0.49915032271406545 1104 20-21 0.3739720919908578 1104 22-23 0.24348015453205818 1104 24-25 0.32342507578945856 1104 26-27 0.02600100894662205 1104 28-29 0.25844027709489126 1104 30-31 0.31968651485305344 1104 32-33 0.05596609994432811 1104 34-35 0.01536823363358053 1104 36-37 0.17257968280728164 1104 38-39 0.38020514872533084 1104 40-41 0.32920266422431865 1104 42-43 0.4288698752868072 1104 44-45 0.49499619862648103 1104 46-47 0.4896920136611911 1104 48-49 0.24884718229767344 1104 50-51 0.2609808468983843 1104 52-53 0.5210162208039648 1104 54-55 0.6332237972055808 1104 56-57 0.37874450130575354 1104 58-59 0.5280992695899229 1104 60-61 0.39177090499845235 1104 62-63 0.6952012988161869 1104 64-65 0.552141946572327 1104 66-67 0.6606071812271779 1104 68-69 0.47776682267004134 1104 70-71 0.5426671083880379 1104 72-73 0.5383579496836255 1104 74-75 0.36331563362364605 1104 76-77 0.632163244249071 1104 78-79 0.4935446718970731 1104 80-81 0.45580789404876754 1104 82-83 0.5662194692378542 1104 84-85 0.6050177344088006 1104 86-87 0.6177704636284034 1104 88-89 0.6218388125614283 1104 90-91 0.8270753753944717 1104 92-93 0.8167576729703576 1104 94-95 0.5614447427524816 1104 96-97 0.6333773200256871 1104 98-99 0.7281031928817683 1104 100-101 0.9656129100213668 1105 1 0.34268142281727876 1105 2 0.23847014945224387 1105 3 0.27776259537631276 1105 4 0.34664574746663845 1105 5 0.46928762803834445 1105 6 0.367194326768562 1105 7 0.37516865416773726 1105 8 0.4831360037184709 1105 9 0.5252587202741239 1105 10-11 0.3717711775337733 1105 12-13 0.45570591312256425 1105 14-15 0.33577949043342414 1105 16-17 0.3014150633223309 1105 18-19 0.3090194745970436 1105 20-21 0.33622880285363266 1105 22-23 0.19773555905910456 1105 24-25 0.4870211780777751 1105 26-27 0.28683052418799804 1105 28-29 0.1845413258186781 1105 30-31 0.24508095831748022 1105 32-33 0.29522498485488313 1105 34-35 0.14368453056909658 1105 36-37 0.3079179315863598 1105 38-39 0.15910564154128082 1105 40-41 -0.011541382969049607 1105 42-43 0.16082750360409648 1105 44-45 -0.035980009217574604 1105 46-47 0.22172632166083162 1105 48-49 0.2479182854203188 1105 50-51 0.31175272921374386 1105 52-53 0.12485918399295315 1105 54-55 0.10995965834622012 1105 56-57 0.3915716613287046 1105 58-59 0.388403191298778 1105 60-61 0.029747848649613218 1105 62-63 0.18734222733165495 1105 64-65 0.35216312287838747 1105 66-67 0.3003926529303911 1105 68-69 0.23609371967771153 1105 70-71 0.4482672679453472 1105 72-73 0.22707002502518847 1105 74-75 0.23120150723963562 1105 76-77 0.1386331421101481 1105 78-79 0.2666455187903942 1105 80-81 0.012802153799800209 1105 82-83 0.12436630804842252 1105 84-85 0.32304051900166897 1105 86-87 0.22731275346647806 1105 88-89 0.0809141112148879 1105 90-91 0.10345437803415791 1105 92-93 0.16724063577792947 1105 94-95 0.29019910713751074 1105 96-97 0.50587927078859 1105 98-99 0.3643047833693558 1105 100-101 0.645136137210951 1106 1 0.18610896348797823 1106 2 0.2923911872107645 1106 3 0.3645704658798614 1106 4 0.46585311303419985 1106 5 0.3389929071914537 1106 6 0.2760389134283656 1106 7 0.4192739834083383 1106 8 0.42369561290072255 1106 9 0.6810113354838023 1106 10-11 0.5651281134657893 1106 12-13 0.6962662837699511 1106 14-15 0.8747375269363715 1106 16-17 0.9242459453019976 1106 18-19 0.7655453153391463 1106 20-21 0.7042199393995574 1106 22-23 0.7521606444278035 1106 24-25 0.7133017509026089 1106 26-27 0.3861361525997893 1106 28-29 0.5765604944341334 1106 30-31 0.6930208567211125 1106 32-33 0.3999355350388143 1106 34-35 0.4088252070208682 1106 36-37 0.4796162611010857 1106 38-39 0.6524732802886888 1106 40-41 0.6129105736578992 1106 42-43 0.4628622230295818 1106 44-45 0.5240096940817764 1106 46-47 0.5479159840102668 1106 48-49 0.5740184756173861 1106 50-51 0.5239294872675089 1106 52-53 0.6267114479133795 1106 54-55 0.6219455424749256 1106 56-57 0.615125342927989 1106 58-59 0.5914305228203887 1106 60-61 0.78114725233781 1106 62-63 0.8962723942109605 1106 64-65 0.8269582041520991 1106 66-67 0.8949150112386519 1106 68-69 0.760203820766435 1106 70-71 0.8808295896355958 1106 72-73 0.9824969446787755 1106 74-75 0.7512106955855025 1106 76-77 0.8250683600414668 1106 78-79 0.945561268596645 1106 80-81 0.8529278970743732 1106 82-83 0.9219572439059824 1106 84-85 0.85920630518903 1106 86-87 0.9331960022489838 1106 88-89 1.1100972621914238 1106 90-91 1.1531685324863616 1106 92-93 1.3940476771305619 1106 94-95 1.4728906663811898 1106 96-97 1.618839449546762 1106 98-99 1.8495150154300823 1106 100-101 2.125753253931709 1107 1 0.15582181401995499 1107 2 0.22537646058285787 1107 3 -0.01959082827035985 1107 4 0.035738675137857 1107 5 0.2513302484879816 1107 6 0.14237037462100233 1107 7 0.21829077993828605 1107 8 0.25198438401174883 1107 9 0.29966484442920205 1107 10-11 0.16217866785675028 1107 12-13 0.15209628812069553 1107 14-15 -0.010902857247991449 1107 16-17 0.2084265211830214 1107 18-19 0.2953627816208453 1107 20-21 0.30668833054512135 1107 22-23 0.24935160937130973 1107 24-25 0.24166814334021325 1107 26-27 0.055348996539720474 1107 28-29 0.08869974478680831 1107 30-31 0.18500987437460736 1107 32-33 0.08305512132209714 1107 34-35 0.2275299691139736 1107 36-37 0.43292728036304595 1107 38-39 0.17259384878662587 1107 40-41 0.1822003797199443 1107 42-43 0.2929720464946257 1107 44-45 0.3879820272700769 1107 46-47 0.3063373211406244 1107 48-49 0.30976729893740895 1107 50-51 0.20948895410293034 1107 52-53 0.40065952237711855 1107 54-55 0.38360686958537116 1107 56-57 0.5402965712895949 1107 58-59 0.48523927441895864 1107 60-61 0.48211562370724437 1107 62-63 0.37926409482665235 1107 64-65 0.4841570465890399 1107 66-67 0.4896245657482723 1107 68-69 0.5486189613747499 1107 70-71 0.544466387747633 1107 72-73 0.42965593820538217 1107 74-75 0.5517222382558025 1107 76-77 0.40596016481147146 1107 78-79 0.39551528908471667 1107 80-81 0.4401274646362374 1107 82-83 0.7540735209828071 1107 84-85 0.5668474738267157 1107 86-87 0.5887451854946413 1107 88-89 0.41761944442190924 1107 90-91 0.5087491927455616 1107 92-93 0.5772422479225305 1107 94-95 0.7497120483374466 1107 96-97 0.7727529816961614 1107 98-99 0.8340517265271323 1107 100-101 1.5701452366869226 1108 1 0.032155059610900594 1108 2 0.14777621490416948 1108 3 0.07094352287467842 1108 4 0.26529031153339844 1108 5 0.18701595525291737 1108 6 0.06391902777534142 1108 7 0.08466526445175049 1108 8 0.20052873778905678 1108 9 0.3378292736649726 1108 10-11 0.2436018493957519 1108 12-13 0.41417051818124406 1108 14-15 0.42802098235891606 1108 16-17 0.15127069070131682 1108 18-19 0.28445991244473845 1108 20-21 0.17442808662128328 1108 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-3.299162122853481 2316 70-71 -3.167968381735264 2316 72-73 -2.771012539821097 2316 74-75 -2.2752756484910783 2316 76-77 -2.3801053029075128 2316 78-79 -3.0936424286019175 2316 80-81 -2.5521656306326648 2316 82-83 -2.3006090597736524 2316 84-85 -2.2254165103023205 2316 86-87 -3.0492642594529578 2316 88-89 -3.320430971759574 2316 90-91 -3.227040258239903 2316 92-93 -3.8341423641445758 2316 94-95 -3.962382012990023 2316 96-97 -4.755632536050854 2316 98-99 -6.108529245569304 2316 100-101 -6.335343830462776 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2792.0 3 1052.0 4 1058.0 5 1183.0 6 1355.0 7 1503.0 8 1907.0 9 2440.0 10 3102.0 11 4012.0 12 5022.0 13 6133.0 14 6982.0 15 8205.0 16 10061.0 17 12149.0 18 10486.0 19 8003.0 20 8110.0 21 9012.0 22 9843.0 23 11126.0 24 12896.0 25 15014.0 26 17603.0 27 20985.0 28 25202.0 29 30193.0 30 36727.0 31 45354.0 32 57243.0 33 73911.0 34 97839.0 35 133296.0 36 186645.0 37 246722.0 38 197791.0 39 28722.0 40 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.17587638830402 18.69880010272358 14.47277566072799 22.65254784824441 2 15.445136597949588 20.23298390188095 40.19438512052499 24.12749437964447 3 19.999526417749863 24.260286724453568 29.47657321803579 26.26361363976078 4 12.818322527349293 16.432374575206023 37.82237523400217 32.92692766344251 5 13.810205902056799 37.43412651706104 34.09883150675298 14.656836074129181 6 31.65788043920774 36.146110447394406 17.58940560097944 14.606603512418411 7 28.621752760084533 32.67125848011222 20.531105773323315 18.175882986479934 8 26.301087795624394 34.17197044440711 19.807694727407167 19.719247032561334 9 26.075792901817106 17.42185707412753 20.79560341956256 35.7067466044928 10-11 25.08446020969227 26.454958502722565 27.231829581355687 21.228751706229477 12-13 26.327509611213994 23.85109538972603 27.56523560612453 22.256159392935444 14-15 22.94213471628374 25.53109603062938 26.341201121143108 25.185568131943768 16-17 22.17915436993743 28.833785231193314 26.83233294028212 22.154727458587136 18-19 22.072324152909356 27.56599586355352 28.973076086039274 21.38860389749785 20-21 22.933935815922677 26.26304180703342 28.86259830605415 21.940424070989753 22-23 22.331627688425666 26.355247725498465 29.02371253408515 22.28941205199072 24-25 22.637454184404717 26.54692833900803 28.885058899952202 21.930558576635047 26-27 22.269295694614673 26.548784859474463 29.187012111636147 21.99490733427472 28-29 21.82205529453066 27.352729121019166 28.951555415311965 21.873660169138212 30-31 22.182259963168296 26.593407731698278 28.82846621627121 22.39586608886222 32-33 21.89850173383377 26.756491611233557 28.934752065840552 22.410254589092123 34-35 22.84165522639892 26.699161836228637 28.50951715596239 21.949665781410054 36-37 22.594516846483835 26.415551533859443 28.835018548963493 22.15491307069323 38-39 22.225099647285234 26.91116722912105 28.824128738299258 22.03960438529445 40-41 22.46219918877378 26.744238092860062 28.694991149755023 22.09857156861113 42-43 22.204848491574232 26.940589609423988 28.242546651056205 22.61201524794558 44-45 22.484036284299517 26.74272875037269 28.22568414074852 22.547550824579275 46-47 22.78802365794013 27.192093699588256 27.792494015787 22.227388626684615 48-49 22.722882536831275 27.201599798175664 27.698873357079258 22.3766443079138 50-51 22.29301860528947 27.458606310417622 27.925356897000082 22.323018187292828 52-53 22.812169844543945 27.568627944209833 27.334245314566942 22.284956896679283 54-55 22.313632876169084 27.140122803240324 27.70882210843057 22.83742221216002 56-57 22.66361856784986 27.40096052850156 27.44443256414345 22.49098833950513 58-59 23.065841627014105 27.046179480899806 27.358232996505443 22.529745895580643 60-61 22.590525136242277 27.17117342418248 28.137401321229156 22.100900118346082 62-63 22.372920104720293 27.34669009074797 27.93458570043441 22.34580410409733 64-65 22.116042268614404 27.58967765132277 27.694213570656256 22.60006650940657 66-67 22.367356511824834 27.40933135096722 27.369008278067913 22.85430385914004 68-69 22.306654669604765 27.28067501232017 27.69271637258573 22.719953945489337 70-71 22.540228277765817 27.332478050072545 27.365032084220246 22.762261587941392 72-73 22.53435785436531 27.051335546914302 27.806668264706403 22.60763833401398 74-75 22.220027359335802 27.901787530860634 27.301480200733774 22.57670490906979 76-77 22.409926031973278 27.438050159163506 27.290299072024034 22.861724736839186 78-79 22.494413121589933 27.399657222212564 27.437353160632416 22.668576495565084 80-81 22.579828892372962 27.658379619798644 27.175155580974987 22.586635906853406 82-83 22.640777948891152 27.501922713395295 27.428565509684592 22.428733828028964 84-85 22.388150263888605 27.753496778394616 27.272966078335976 22.5853868793808 86-87 22.497484500081384 27.703977449283084 27.118568828516892 22.679969222118643 88-89 22.314085051522557 27.661147868162928 26.86042575702366 23.164341323290856 90-91 22.420436452741292 27.35860712742597 27.229281220221008 22.991675199611727 92-93 22.751783356376407 27.424378697174046 27.34143102856959 22.482406917879956 94-95 22.33035533603374 27.357015989733068 27.38927588014563 22.923352794087563 96-97 22.07656721967064 27.311157682895477 27.75453504567878 22.857740051755105 98-99 22.114119896151717 27.555425155274794 27.059862978575094 23.270591969998396 100-101 22.006236090727498 27.357055049449848 27.344843383534386 23.291865476288276 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 117.0 1 92.0 2 88.0 3 127.0 4 129.5 5 130.0 6 221.0 7 400.0 8 641.0 9 822.0 10 830.0 11 785.5 12 842.0 13 928.0 14 1032.5 15 1175.0 16 1284.0 17 1382.0 18 1495.5 19 1694.0 20 1903.5 21 2161.5 22 2586.0 23 3177.5 24 3961.0 25 5021.5 26 6312.5 27 7932.5 28 9922.5 29 12309.0 30 15176.5 31 18407.5 32 21993.0 33 24703.5 34 27035.5 35 29304.5 36 31924.0 37 35067.0 38 37339.0 39 38754.5 40 39958.5 41 42523.5 42 45137.0 43 47048.5 44 48976.5 45 50993.5 46 52260.5 47 55505.0 48 63689.5 49 62696.5 50 53596.0 51 48996.5 52 46231.5 53 44623.0 54 43114.5 55 39699.0 56 35438.0 57 31528.5 58 28491.5 59 24823.5 60 20441.5 61 16907.5 62 13979.5 63 10810.5 64 8019.5 65 6294.5 66 4884.0 67 3916.5 68 2928.5 69 2003.5 70 1528.5 71 1185.5 72 931.5 73 861.5 74 698.0 75 543.5 76 414.0 77 270.5 78 185.5 79 129.0 80 85.0 81 57.0 82 41.5 83 22.5 84 11.5 85 7.0 86 9.0 87 9.0 88 5.5 89 4.0 90 4.0 91 4.5 92 5.0 93 5.0 94 2.5 95 1.0 96 1.5 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.036398851512660806 2 0.02611543614627899 3 0.021380626193412546 4 0.054894202891045366 5 0.05962901284391182 6 0.05274874213115276 7 0.05445031445796413 8 0.045128657363258315 9 0.047422080934178004 10-11 0.056151886784775513 12-13 0.049197634666502926 14-15 0.042169401142716785 16-17 0.053414574780774604 18-19 0.057187626461965055 20-21 0.012724801748328575 22-23 0.021898496032007313 24-25 0.005696568224542444 26-27 0.010320406069138583 28-29 0.0055116147107585976 30-31 0.010209433960868276 32-33 0.00458684714183937 34-35 0.007805038281678283 36-37 0.009839526933300584 38-39 0.01287276455935565 40-41 0.0024783770847035306 42-43 0.0024783770847035306 44-45 0.0023674049764332234 46-47 7.028233523786131E-4 48-49 0.0015166188130275337 50-51 4.068977303244603E-4 52-53 0.004068977303244603 54-55 0.007250177740326747 56-57 0.01831039786460071 58-59 0.011837024882166116 60-61 0.010838275907733352 62-63 0.006695317198975209 64-65 1.8495351378384556E-4 66-67 0.007953001092705358 68-69 0.01842136997287102 70-71 0.0068062893072455164 72-73 0.002589349192973838 74-75 0.0036990702756769115 76-77 0.006917261415515824 78-79 0.006399391576921057 80-81 0.010653322393949505 82-83 0.006325410171407518 84-85 0.019864007380385015 86-87 0.005252679791461214 88-89 0.007287168443083515 90-91 0.004956754169407061 92-93 0.017496602403951792 94-95 0.012391885423517653 96-97 0.009876517636057354 98-99 0.021935486734764083 100-101 0.038766256489094035 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1351691.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.09413427749531 #Duplication Level Percentage of deduplicated Percentage of total 1 82.9820523294759 54.01644856957047 2 10.57211069455571 13.763647862959072 3 2.9071591136000485 5.677170171201775 4 1.1550339097039575 3.0074372965331926 5 0.6383109948237515 2.0775150803929447 6 0.3588435558793999 1.401516636661653 7 0.24124999268893615 1.0992771592976862 8 0.1855782523643992 0.9664044542712892 9 0.1302295024683592 0.7629459048510104 >10 0.7472384171831323 8.963701779745037 >50 0.05447394537049325 2.429728266025668 >100 0.024639410076651887 3.068517190593531 >500 0.001596975752981611 0.6788180142892544 >1k 0.0013688363596985238 1.701054680524232 >5k 1.1406969664154364E-4 0.3858169330832384 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5196 0.38440738304834465 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3851 0.2849023926326357 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3086 0.22830661741477898 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3004 0.22224014216266882 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2087 0.15439919330675428 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1926 0.14248818701907462 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1764 0.13050319932588142 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1521 0.11252571778609165 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 7.398140551353823E-5 0.0 10-11 0.0 0.0 0.0 7.398140551353823E-5 0.0 12-13 0.0 0.0 0.0 7.398140551353823E-5 0.0 14-15 0.0 0.0 0.0 7.398140551353823E-5 7.398140551353823E-5 16-17 0.0 0.0 0.0 7.398140551353823E-5 1.4796281102707646E-4 18-19 0.0 0.0 0.0 7.398140551353823E-5 1.4796281102707646E-4 20-21 0.0 0.0 0.0 7.398140551353823E-5 1.4796281102707646E-4 22-23 0.0 0.0 0.0 7.398140551353823E-5 1.4796281102707646E-4 24-25 0.0 0.0 0.0 2.589349192973838E-4 1.4796281102707646E-4 26-27 0.0 0.0 0.0 3.699070275676911E-4 1.4796281102707646E-4 28-29 0.0 0.0 0.0 9.98748974432766E-4 1.4796281102707646E-4 30-31 0.0 0.0 0.0 0.0021084700571358393 1.4796281102707646E-4 32-33 0.0 0.0 0.0 0.00377305168119045 1.4796281102707646E-4 34-35 0.0 0.0 0.0 0.005659577521785674 2.219442165406147E-4 36-37 0.0 0.0 0.0 0.008285917417516282 2.219442165406147E-4 38-39 0.0 0.0 0.0 0.010727303799463043 2.219442165406147E-4 40-41 0.0 0.0 0.0 0.013723550722761341 2.959256220541529E-4 42-43 0.0 0.0 0.0 0.018680304892168403 2.959256220541529E-4 44-45 0.0 0.0 0.0 0.027484092148279453 2.959256220541529E-4 46-47 0.0 0.0 0.0 0.03854431227255342 2.959256220541529E-4 48-49 0.0 0.0 0.0 0.05885220808601967 2.959256220541529E-4 50-51 0.0 0.0 3.6990702756769115E-5 0.07834630843883698 2.959256220541529E-4 52-53 0.0 0.0 7.398140551353823E-5 0.10042975798462814 2.959256220541529E-4 54-55 0.0 0.0 7.398140551353823E-5 0.1332035206271256 2.959256220541529E-4 56-57 0.0 0.0 7.398140551353823E-5 0.168381678948813 2.959256220541529E-4 58-59 0.0 0.0 7.398140551353823E-5 0.21480501090855825 2.959256220541529E-4 60-61 0.0 0.0 7.398140551353823E-5 0.2648164410357101 2.959256220541529E-4 62-63 0.0 0.0 7.398140551353823E-5 0.3374661812500046 2.959256220541529E-4 64-65 0.0 0.0 7.398140551353823E-5 0.4030506972377562 2.959256220541529E-4 66-67 0.0 0.0 7.398140551353823E-5 0.49219829088156986 2.959256220541529E-4 68-69 0.0 0.0 7.398140551353823E-5 0.6143785820871782 2.959256220541529E-4 70-71 0.0 0.0 7.398140551353823E-5 0.6851048057581208 2.959256220541529E-4 72-73 0.0 0.0 7.398140551353823E-5 0.7865703034199385 3.699070275676911E-4 74-75 0.0 0.0 7.398140551353823E-5 0.9155938746355492 3.699070275676911E-4 76-77 0.0 0.0 7.398140551353823E-5 1.0182060840828266 3.699070275676911E-4 78-79 0.0 0.0 7.398140551353823E-5 1.1080934917817755 3.699070275676911E-4 80-81 0.0 0.0 7.398140551353823E-5 1.2641942574153413 3.699070275676911E-4 82-83 0.0 0.0 7.398140551353823E-5 1.4551402650457834 3.699070275676911E-4 84-85 0.0 0.0 7.398140551353823E-5 1.6795998493738584 3.699070275676911E-4 86-87 0.0 0.0 7.398140551353823E-5 1.9949455903753148 4.068977303244602E-4 88-89 0.0 0.0 7.398140551353823E-5 2.3468381457004597 4.438884330812294E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 5740 0.0 26.160475 1 TACATGG 5990 0.0 24.03644 2 ACATGGG 5790 0.0 22.979141 3 GTATTAG 455 0.0 22.976385 1 TACACCG 125 2.250541E-4 22.808441 5 GAGTACT 4400 0.0 21.334839 12-13 CCTATAC 385 0.0 20.98179 3 GTACTTT 4560 0.0 20.324533 14-15 CATGGGG 2555 0.0 20.272438 4 CATGGGA 3150 0.0 19.611156 4 CATTCCG 195 1.02924205E-5 19.495115 9 GTGTAGC 780 0.0 19.495115 1 GTATAGA 415 0.0 19.465755 1 GTATAGG 470 0.0 19.209946 1 AGAGTAC 7490 0.0 19.034172 10-11 AGTACTT 4595 0.0 18.67096 12-13 GTGTAGG 550 0.0 18.14375 1 GTCCTAC 500 0.0 18.057348 1 GTATCAA 11710 0.0 17.61178 1 CGTACAC 135 0.009381216 17.599104 3 >>END_MODULE