FastQCFastQC Report
Thu 26 May 2016
SRR936509_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936509_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1351691
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT55120.40778550719062273No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48690.36021546344541766No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43320.32048744868464757No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT28460.2105510800915298No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24470.18103249929162804No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT20040.1482587366491306No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC19120.14145244734188508No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT15510.11474515995149778No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT15000.11097210827030735No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC14860.10993636859311781No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCGT1452.3342593E-522.931649
GTACATG55900.022.184331
GAGTACT57050.022.14700712-13
TACATGG57000.021.4981842
GTACTTT59750.020.94670314-15
ACATGGG56100.020.911783
TAAGGTG6850.020.8011635
AGTACTT58750.020.17212912-13
AGAGTAC91050.019.51177410-11
GTATAGG3900.019.4927441
GTAAGGT6600.019.4301764
GTATCAA130050.018.8885141
CATGGGG25950.018.6689434
GTACCTG11250.018.5830821
ACTTTTT71600.017.64548316-17
GGACCGC2202.9180641E-517.2725456
TACTTTT68350.017.26874714-15
TACCTGG11350.017.157142
CAACGCA145750.016.8150445
ATCAACG145750.016.8150443
Produced by FastQC (version 0.11.5)