##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936509_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1351691 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.880577735591935 31.0 31.0 34.0 30.0 34.0 2 32.028690728872206 31.0 31.0 34.0 30.0 34.0 3 32.13651048945358 33.0 31.0 34.0 30.0 34.0 4 35.88144627729267 37.0 35.0 37.0 35.0 37.0 5 35.657108022469636 37.0 35.0 37.0 33.0 37.0 6 35.75942134703863 37.0 35.0 37.0 33.0 37.0 7 35.71315855472886 37.0 35.0 37.0 33.0 37.0 8 35.68361851932136 37.0 35.0 37.0 33.0 37.0 9 37.32875856982106 39.0 37.0 39.0 34.0 39.0 10-11 37.19128558228175 39.0 37.0 39.0 33.0 39.0 12-13 37.09682908297829 39.0 37.0 39.0 33.0 39.0 14-15 38.202376134782284 40.0 37.5 41.0 33.0 41.0 16-17 38.13356122072278 40.0 37.0 41.0 33.0 41.0 18-19 38.28840615199776 40.0 38.0 41.0 33.5 41.0 20-21 38.31777455054447 40.0 38.0 41.0 34.0 41.0 22-23 38.234447443979434 40.0 38.0 41.0 33.5 41.0 24-25 38.10594359213755 40.0 37.0 41.0 33.0 41.0 26-27 37.89635501013176 40.0 37.0 41.0 33.0 41.0 28-29 37.81513452408872 40.0 37.0 41.0 33.0 41.0 30-31 37.78909935776742 40.0 37.0 41.0 33.0 41.0 32-33 37.644765334680784 40.0 37.0 41.0 33.0 41.0 34-35 37.46866036690338 40.0 36.0 41.0 32.0 41.0 36-37 37.22162498677582 39.0 36.0 41.0 31.5 41.0 38-39 37.051380456036185 39.0 36.0 41.0 31.0 41.0 40-41 36.83943963524208 39.0 35.5 41.0 31.0 41.0 42-43 36.5946340546767 39.0 35.0 40.5 30.0 41.0 44-45 36.35102623306658 39.0 35.0 40.0 30.0 41.0 46-47 36.00449030140764 39.0 35.0 40.0 29.5 41.0 48-49 35.64852839887223 39.0 35.0 40.0 28.0 41.0 50-51 35.33735594895579 38.0 34.5 40.0 27.0 41.0 52-53 35.07073029264825 38.0 34.0 40.0 26.5 41.0 54-55 34.80578549387397 38.0 34.0 40.0 26.0 41.0 56-57 34.49510723974636 38.0 34.0 40.0 25.5 41.0 58-59 34.15588473992946 37.5 33.0 40.0 25.0 41.0 60-61 33.794145629437494 37.0 33.0 40.0 23.5 41.0 62-63 33.406165314409876 36.5 32.5 39.0 23.0 40.5 64-65 33.0039476477982 36.0 32.0 39.0 22.0 40.0 66-67 32.53810671225894 35.5 31.0 39.0 21.5 40.0 68-69 32.24957960066317 35.0 31.0 38.0 20.5 40.0 70-71 31.81147614358607 35.0 31.0 37.5 19.5 40.0 72-73 31.35164397780262 35.0 30.5 37.0 18.0 39.0 74-75 30.82490080943056 34.0 30.0 36.5 17.0 39.0 76-77 29.408532719386308 32.5 28.5 35.0 13.0 37.0 78-79 30.20130007523909 34.0 29.5 36.0 13.5 37.5 80-81 29.995282945584457 34.0 30.0 35.0 9.0 37.0 82-83 29.653459259549706 34.0 29.0 35.0 7.0 37.0 84-85 29.22230561570655 34.0 29.0 35.0 6.0 36.0 86-87 28.85297786254403 34.0 29.0 35.0 2.0 36.0 88-89 28.442817182329392 33.0 28.5 35.0 2.0 35.0 90-91 27.93785820871782 33.0 27.0 35.0 2.0 35.0 92-93 27.437869305928647 33.0 26.5 34.5 2.0 35.0 94-95 26.8400840132841 32.5 25.5 34.0 2.0 35.0 96-97 26.057983296478263 32.0 24.5 34.0 2.0 35.0 98-99 25.077090474080244 32.0 20.5 34.0 2.0 35.0 100-101 21.80046992988782 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.29305614277704706 1101 2 0.37307653544021946 1101 3 0.3010901614547308 1101 4 0.1425334908734044 1101 5 0.1625908560059557 1101 6 0.09482935420707861 1101 7 0.1915503530649545 1101 8 0.23822798582912696 1101 9 0.309384620273633 1101 10-11 0.29203325954536297 1101 12-13 0.34754090981442687 1101 14-15 0.42895999046255895 1101 16-17 0.39408484812909705 1101 18-19 0.39703060615446617 1101 20-21 0.2437048830902384 1101 22-23 0.2445406365750884 1101 24-25 0.19922146494626247 1101 26-27 0.23715615249705024 1101 28-29 0.2214496321747177 1101 30-31 0.34096582026518263 1101 32-33 0.30594788504348713 1101 34-35 0.2122507407593801 1101 36-37 0.2884513899316019 1101 38-39 0.17632345883593814 1101 40-41 0.1134456992709616 1101 42-43 0.2238184847535294 1101 44-45 0.1698946517462332 1101 46-47 0.22561569704106432 1101 48-49 0.19939550583804788 1101 50-51 0.15105931948563267 1101 52-53 0.2834695549441548 1101 54-55 0.26041455274931735 1101 56-57 0.21690078545460523 1101 58-59 0.18782902435754067 1101 60-61 0.1826325003874132 1101 62-63 0.2541251957099391 1101 64-65 0.2774211297609952 1101 66-67 0.26478985449339376 1101 68-69 0.26309958691383173 1101 70-71 0.41164885378004 1101 72-73 0.3708812858822661 1101 74-75 0.31469520709007526 1101 76-77 0.12981889461021368 1101 78-79 0.1536587693914555 1101 80-81 0.3615916620736819 1101 82-83 0.5119843115878702 1101 84-85 0.47956955459039463 1101 86-87 0.601765848840774 1101 88-89 0.36697193967076913 1101 90-91 0.4418100210745486 1101 92-93 0.3439875283723275 1101 94-95 0.3476525492940361 1101 96-97 0.32511312046792895 1101 98-99 0.48028215537902597 1101 100-101 0.8546998677503694 1102 1 0.22448582913381188 1102 2 0.2466674822233017 1102 3 0.23774456855682047 1102 4 0.1024585676989247 1102 5 0.12599205594742102 1102 6 0.11394686067018256 1102 7 0.16913193445122232 1102 8 0.19678610494526794 1102 9 0.11297898152430719 1102 10-11 0.2983424151963163 1102 12-13 0.2210046432908399 1102 14-15 0.3693088514937273 1102 16-17 0.43571696363565593 1102 18-19 0.25204021544181643 1102 20-21 0.189331535448666 1102 22-23 0.20853810011345075 1102 24-25 0.234010011846415 1102 26-27 -0.015407332211012204 1102 28-29 -0.06702000345281789 1102 30-31 0.16528658510592464 1102 32-33 0.29320431155927196 1102 34-35 0.2122770809379091 1102 36-37 0.21254914126080848 1102 38-39 0.08654637479126137 1102 40-41 0.17784724065919022 1102 42-43 0.26148728139759214 1102 44-45 0.32823580583627887 1102 46-47 0.39607138212959825 1102 48-49 0.3620020128377064 1102 50-51 0.18241330221817975 1102 52-53 0.21342458152822275 1102 54-55 0.25020178264054493 1102 56-57 0.04953177906467232 1102 58-59 -0.1223082372394515 1102 60-61 0.07251631093323851 1102 62-63 -0.049879096763604025 1102 64-65 0.06485062100531991 1102 66-67 0.10079317140476007 1102 68-69 0.010787992357464304 1102 70-71 0.049935961725996236 1102 72-73 -0.16645562451234852 1102 74-75 -0.1970562196695944 1102 76-77 -0.448202470201263 1102 78-79 -0.5284222998246833 1102 80-81 -0.10820981102519056 1102 82-83 -0.10097115399917911 1102 84-85 -0.0939772967827075 1102 86-87 0.08860629564824762 1102 88-89 0.039798045714377395 1102 90-91 0.05232697146721321 1102 92-93 0.1493997521662891 1102 94-95 0.02147431408599587 1102 96-97 0.1317931848550309 1102 98-99 0.4381443084447305 1102 100-101 0.7541457484391145 1103 1 0.19661419156891924 1103 2 0.2839152405998959 1103 3 0.2593642004586627 1103 4 0.19950623169137316 1103 5 0.08315486859489596 1103 6 0.17084539771595786 1103 7 0.232133350213374 1103 8 0.20150531211846356 1103 9 0.22766892961302432 1103 10-11 0.30245473232703546 1103 12-13 0.2624062478212821 1103 14-15 0.3111691629613915 1103 16-17 0.4687278178121588 1103 18-19 0.23819500543709893 1103 20-21 0.3390729228955749 1103 22-23 0.25727429643247035 1103 24-25 0.350220488486336 1103 26-27 0.405298661182087 1103 28-29 0.2233547015967403 1103 30-31 0.23786799142965975 1103 32-33 0.3413704276517606 1103 34-35 0.2618004410134134 1103 36-37 0.49202908463031747 1103 38-39 0.40435194345572967 1103 40-41 0.3834045790263403 1103 42-43 0.5028218731861713 1103 44-45 0.43911924141909964 1103 46-47 0.5032549592886326 1103 48-49 0.4384418429462542 1103 50-51 0.4852344442663181 1103 52-53 0.39582099493077294 1103 54-55 0.3872796495589057 1103 56-57 0.3265294194610888 1103 58-59 0.5498040798912669 1103 60-61 0.3270694020139473 1103 62-63 0.3857027814808518 1103 64-65 0.33903750160938273 1103 66-67 0.5314793441277459 1103 68-69 0.7328550050993243 1103 70-71 0.6047467215183495 1103 72-73 0.4227071881351243 1103 74-75 0.595324148428567 1103 76-77 0.3922666189917514 1103 78-79 0.4058797294344032 1103 80-81 0.5788123692485208 1103 82-83 0.7143898653495704 1103 84-85 0.3417216177996387 1103 86-87 0.2905392829063871 1103 88-89 0.3241057380220731 1103 90-91 0.4774794018589077 1103 92-93 0.6874771471639285 1103 94-95 0.7739613362662716 1103 96-97 0.6945765732457794 1103 98-99 1.1339168426589872 1103 100-101 1.3400305997840931 1104 1 0.2131495012281377 1104 2 0.25403525313851816 1104 3 0.16980782889745427 1104 4 0.04267342826415188 1104 5 0.029560463209257648 1104 6 0.06092346557379358 1104 7 0.035087390187705125 1104 8 0.020162485255958984 1104 9 0.11978527012621498 1104 10-11 0.15556363922775063 1104 12-13 0.10801287836476803 1104 14-15 0.037181582169374394 1104 16-17 0.1937882718894457 1104 18-19 0.0557158658254977 1104 20-21 0.11134987720422629 1104 22-23 0.21746267432270372 1104 24-25 0.2014721117902667 1104 26-27 0.17367595433212557 1104 28-29 0.21125306411222766 1104 30-31 0.055292199929056096 1104 32-33 0.28738978788342706 1104 34-35 0.07209927820536421 1104 36-37 0.15056542424788688 1104 38-39 0.29590628495409277 1104 40-41 0.08339599604006054 1104 42-43 0.1801773431606435 1104 44-45 0.1519049055929642 1104 46-47 0.12885427563512053 1104 48-49 0.06204356252889198 1104 50-51 -0.016568356026247955 1104 52-53 -0.026257058000126676 1104 54-55 -0.04465204479722473 1104 56-57 0.1769790933076152 1104 58-59 0.051757269030431985 1104 60-61 0.11844200810711669 1104 62-63 -0.18625734868199828 1104 64-65 0.06041846678485285 1104 66-67 -0.1600522337983108 1104 68-69 0.11150762552235705 1104 70-71 0.06313416498259627 1104 72-73 0.13736095793197123 1104 74-75 0.20617005770267127 1104 76-77 0.06108313713543723 1104 78-79 0.05020712184853693 1104 80-81 0.3040258243856293 1104 82-83 0.15367979116271968 1104 84-85 0.20822408820699678 1104 86-87 0.21298155299430732 1104 88-89 0.19344880371550133 1104 90-91 0.14137451030307346 1104 92-93 -0.00525269028177533 1104 94-95 0.2961121579939139 1104 96-97 0.47150140121287976 1104 98-99 0.5746498296874769 1104 100-101 0.9983493793580749 1105 1 0.02272127102699173 1105 2 0.14162482533740928 1105 3 0.09802412440267716 1105 4 0.057044710491467754 1105 5 0.005092493067451187 1105 6 0.07279808912820585 1105 7 0.08616956696893396 1105 8 0.087043855033194 1105 9 0.05983804799669201 1105 10-11 0.042419589491643706 1105 12-13 -0.09121662988405177 1105 14-15 0.19754700654572588 1105 16-17 0.17746443889065233 1105 18-19 0.08990606481126662 1105 20-21 0.14003364065893464 1105 22-23 0.13749172519509756 1105 24-25 0.2337788909098748 1105 26-27 0.33951463935105863 1105 28-29 0.24738727183231646 1105 30-31 0.2780987239794612 1105 32-33 0.3953581577131118 1105 34-35 0.3681269528165245 1105 36-37 0.24789637534611586 1105 38-39 0.4264981408566726 1105 40-41 0.2803298814671038 1105 42-43 0.19685159833473875 1105 44-45 0.24514501550316226 1105 46-47 0.24137932565569997 1105 48-49 0.10313711809822479 1105 50-51 0.083882226500954 1105 52-53 0.14382582757153273 1105 54-55 0.23432055870445367 1105 56-57 -0.046232879529505055 1105 58-59 0.017267304136218797 1105 60-61 -0.019139419358666032 1105 62-63 0.17169433437742754 1105 64-65 -0.13997156660458643 1105 66-67 0.09625556400889224 1105 68-69 0.18183858774509787 1105 70-71 -0.022775287834374325 1105 72-73 -0.09232448850270103 1105 74-75 -0.18667346330120438 1105 76-77 -0.15671636257929222 1105 78-79 0.021576795789044922 1105 80-81 0.16781390399264495 1105 82-83 0.07131945545668472 1105 84-85 0.0509230699961698 1105 86-87 0.0753897956231242 1105 88-89 0.07348522855113515 1105 90-91 0.2029663725625248 1105 92-93 0.01926017521808987 1105 94-95 0.1466785546527447 1105 96-97 0.3711568988679943 1105 98-99 0.8368683199002938 1105 100-101 0.9214511797811262 1106 1 0.20698483272089874 1106 2 0.21029163527232697 1106 3 0.24520052710605 1106 4 0.19763951628402054 1106 5 0.14733840907615559 1106 6 0.10742952308548581 1106 7 0.19230849881950007 1106 8 0.18239790247416465 1106 9 0.26213521906914394 1106 10-11 0.20683344748009347 1106 12-13 0.27155466321909927 1106 14-15 0.422311840732462 1106 16-17 0.2314523163658322 1106 18-19 0.1840976088256312 1106 20-21 0.1402570037627342 1106 22-23 0.25735416228961583 1106 24-25 0.19485596640998182 1106 26-27 0.23707812016044727 1106 28-29 0.09668824684610655 1106 30-31 0.34081468395008585 1106 32-33 0.18454209553694767 1106 34-35 0.2696515329108067 1106 36-37 0.2928940994114626 1106 38-39 0.3133767853133804 1106 40-41 0.4278815172634154 1106 42-43 0.41227663970767736 1106 44-45 0.29022544314197773 1106 46-47 0.11887608519855064 1106 48-49 0.1488736748544497 1106 50-51 0.0508750399623068 1106 52-53 0.0614429155258307 1106 54-55 -0.053216710783132726 1106 56-57 0.2385863824923362 1106 58-59 0.13080196602878402 1106 60-61 0.12826182707513567 1106 62-63 0.20616036118777714 1106 64-65 0.1708876875657097 1106 66-67 0.2790968780607983 1106 68-69 0.31855901666994413 1106 70-71 0.31183173693524324 1106 72-73 0.19090627265783766 1106 74-75 0.16130129328377407 1106 76-77 0.005795736255496564 1106 78-79 0.019194679051366847 1106 80-81 0.2413966058739696 1106 82-83 0.323171930895203 1106 84-85 0.3179621213121493 1106 86-87 0.35056065993681074 1106 88-89 0.29152801781481585 1106 90-91 0.4020192791595214 1106 92-93 0.29831704989446806 1106 94-95 0.47609069511429425 1106 96-97 0.6121959349546735 1106 98-99 0.682788389752023 1106 100-101 0.8785787894913284 1107 1 -0.027698551371180713 1107 2 0.04732475370500566 1107 3 -0.01629105485284299 1107 4 -0.020308314274068096 1107 5 -0.02633560112832356 1107 6 -0.07491632194872722 1107 7 -0.049276756094869256 1107 8 -0.19846736931583564 1107 9 -0.09835239447883026 1107 10-11 -0.09870123326718527 1107 12-13 -0.11769568945529585 1107 14-15 0.061635529904364716 1107 16-17 -0.044856534113407065 1107 18-19 -0.05793590512971747 1107 20-21 0.0533062858890645 1107 22-23 -0.11281304492472088 1107 24-25 -0.18251964170614343 1107 26-27 -0.004884529314168162 1107 28-29 -0.06090941429642527 1107 30-31 -0.05466350297393774 1107 32-33 0.04993831038486718 1107 34-35 -4.137668589265786E-5 1107 36-37 0.141394903640915 1107 38-39 0.1536935660224188 1107 40-41 0.28432326336422165 1107 42-43 0.08605529862209949 1107 44-45 0.16764801957673825 1107 46-47 0.10057656905463119 1107 48-49 0.007658892853363852 1107 50-51 0.21196566080107004 1107 52-53 0.19634746271827908 1107 54-55 0.22549988403024912 1107 56-57 -0.007761624732545158 1107 58-59 0.20532750361002172 1107 60-61 0.32361789637070615 1107 62-63 0.2595460353561876 1107 64-65 0.21357776917521676 1107 66-67 0.4831750465270943 1107 68-69 0.5854865635064144 1107 70-71 0.17975764894463353 1107 72-73 0.2566009423304756 1107 74-75 0.1947707523405846 1107 76-77 -0.07760763755299394 1107 78-79 0.24907740657578614 1107 80-81 0.4563771923022699 1107 82-83 0.46588145903664113 1107 84-85 0.7398612234756605 1107 86-87 0.7391163758987851 1107 88-89 0.4453525573604935 1107 90-91 0.6030370908643583 1107 92-93 0.4043608012952795 1107 94-95 0.5872053769162946 1107 96-97 0.9183889961683995 1107 98-99 1.1225222689066605 1107 100-101 1.4685742080202502 1108 1 -0.025805948054518524 1108 2 0.0342642991994353 1108 3 0.014710085798199657 1108 4 -0.1358650397281309 1108 5 -0.10720688304461845 1108 6 0.07183545483464115 1108 7 -0.013785837432067183 1108 8 0.05938557444129344 1108 9 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-1.2889577929806926 2316 98-99 -1.4116197072006393 2316 100-101 -1.321121526658665 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 8.0 4 24.0 5 36.0 6 85.0 7 237.0 8 529.0 9 1010.0 10 1606.0 11 2582.0 12 3702.0 13 5148.0 14 6796.0 15 8786.0 16 11085.0 17 13662.0 18 12512.0 19 8435.0 20 7984.0 21 8196.0 22 8769.0 23 9758.0 24 11497.0 25 13916.0 26 17028.0 27 21172.0 28 26363.0 29 33052.0 30 40628.0 31 50645.0 32 63209.0 33 80715.0 34 105604.0 35 143944.0 36 203030.0 37 264127.0 38 157826.0 39 7984.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.81713656439829 19.46298741281927 15.326713123586458 22.393162899195985 2 15.77769047455222 20.78793380639844 39.265143595309596 24.169232123739746 3 20.000458704057458 24.089953345358282 30.12635077247243 25.78323717811183 4 13.114578239360084 17.12928883262964 37.443727393856086 32.31240553415419 5 13.672530722855466 37.514176791713425 34.10405265739476 14.709239828036349 6 31.679916785407265 36.557011021790565 17.11003886997424 14.653033322827936 7 28.422250351596627 32.880961699086555 20.56609091870849 18.130697030608328 8 26.493111221425607 34.0944047123196 19.587168960953356 19.825315105301435 9 25.927671373950922 17.172019081665265 20.601347672202557 36.29896187218125 10-11 25.043972909358658 26.551018377439412 27.07636286426307 21.32864584893886 12-13 26.354622311905025 23.807786990609817 27.690258956000967 22.147331741484194 14-15 22.757308129803114 25.499745683556363 26.6097121137858 25.133234072854727 16-17 21.916395949564127 28.76580375050771 27.171612324724393 22.14618797520377 18-19 21.77354143809495 27.25397298642959 29.758724442198698 21.213761133276762 20-21 22.555265959453752 25.85764793876707 29.882865240650414 21.704220861128764 22-23 22.01136000029593 25.950923762967733 29.95100514362762 22.086711093108725 24-25 22.393466479195638 26.11481084032373 29.905291617699664 21.58643106278097 26-27 22.085198061299458 26.155884722634276 30.032397991457703 21.726519224608566 28-29 21.584762017501188 26.92952359109145 29.895440479867712 21.59027391153965 30-31 21.938306892672756 26.194509107037234 29.8190133793306 22.04817062095941 32-33 21.816959103697823 26.281824120524945 29.79376882369957 22.107447952077663 34-35 22.572903888355356 26.191832633655277 29.519841310945612 21.71542216704376 36-37 22.455286092796573 25.88760056143487 29.817459677389518 21.83965366837904 38-39 22.07519989700874 26.426395653650452 29.74534819110502 21.753056258235787 40-41 22.279550481996345 26.394386203733163 29.537276110293192 21.788787203977304 42-43 22.043922199357766 26.553255176592483 29.03970635181039 22.363116272239363 44-45 22.32599431818182 26.332600911458332 28.948826645359848 22.392578125 46-47 22.703780233470866 26.812454640083626 28.472895762264837 22.010869364180667 48-49 22.717424716861714 26.937615804918398 28.236517431449222 22.10844204677067 50-51 22.195165347998547 27.041136600799227 28.57034526305977 22.193352788142455 52-53 22.77848554526703 27.22743306788353 27.81788871231693 22.1761926745325 54-55 22.30828132918533 26.858036999086305 28.242968804909534 22.590712866818826 56-57 22.692314379175148 26.966719403761335 27.995962042608664 22.345004174454854 58-59 23.05699347701122 26.713699460153688 27.858607171366618 22.370699891468476 60-61 22.514557256322014 26.911568158958836 28.687625564543367 21.88624902017578 62-63 22.441392428522864 26.98587677104492 28.42372424283564 22.149006557596575 64-65 22.107484335035288 27.207689057941685 28.2333382411587 22.45148836586432 66-67 22.287014907208647 27.10286562131125 27.920488968576823 22.68963050290328 68-69 22.37724987487594 26.880347365847218 28.086273270948247 22.656129488328595 70-71 22.638437807485445 26.949041570182512 27.870517648277342 22.5420029740547 72-73 22.532626065340907 26.719933712121215 28.282211766098484 22.465228456439394 74-75 22.311752380839643 27.529290690310322 27.610378330068126 22.54857859878191 76-77 22.45962184249134 27.00766243556992 27.708763822828097 22.823951899110646 78-79 22.45392591080043 27.049035089958267 27.793308127187828 22.703730872053473 80-81 22.651417038344913 27.23178036747224 27.548839760897785 22.56796283328506 82-83 22.57754020586141 27.134642239505784 27.78289297241309 22.504924582219715 84-85 22.431952531388028 27.38748770003625 27.576925637933456 22.603634130642263 86-87 22.453399721082672 27.249594016224556 27.45648852702798 22.840517735664793 88-89 22.246576497889688 27.32716202153559 27.15107136000071 23.27519012057401 90-91 22.300869306574665 27.045726458099185 27.576714510960482 23.076689724365668 92-93 22.5780009070174 26.955432597731864 27.66288397501485 22.803682520235885 94-95 22.209834226921277 26.92641752739395 27.731127563223783 23.132620682460992 96-97 22.050723581560412 26.869138308725667 28.086624117116564 22.993513992597357 98-99 22.052380989372654 26.96576676954059 27.391356297041884 23.59049594404487 100-101 21.569543552808266 26.90977963725414 27.64684992620049 23.873826883737102 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 162.0 1 125.0 2 118.5 3 156.0 4 153.5 5 150.5 6 228.0 7 445.5 8 727.0 9 945.0 10 947.5 11 876.0 12 944.5 13 1099.0 14 1275.5 15 1391.5 16 1518.0 17 1714.5 18 1880.5 19 2055.0 20 2259.0 21 2550.5 22 3007.5 23 3653.5 24 4442.0 25 5406.5 26 6709.5 27 8510.5 28 10604.0 29 12911.5 30 15660.0 31 18823.0 32 22492.5 33 25117.5 34 27060.0 35 29409.0 36 32224.0 37 35051.5 38 37182.5 39 38359.5 40 39719.0 41 42479.5 42 44768.5 43 46589.5 44 48384.0 45 50121.0 46 51292.5 47 55009.5 48 63465.0 49 62378.5 50 53217.0 51 48380.5 52 45803.0 53 44535.0 54 42658.5 55 38926.0 56 34900.5 57 31271.0 58 28346.5 59 24718.5 60 20372.5 61 16924.0 62 14010.0 63 10722.5 64 7784.0 65 6082.0 66 4783.0 67 3790.0 68 2826.5 69 1972.0 70 1463.5 71 1134.5 72 927.5 73 849.5 74 726.0 75 626.5 76 491.0 77 316.5 78 195.0 79 139.0 80 108.0 81 76.0 82 57.0 83 32.0 84 20.5 85 14.5 86 8.0 87 4.0 88 1.5 89 2.5 90 2.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03514116761893066 2 0.005918512441083058 3 0.004216940114271679 4 8.877768661624587E-4 5 7.398140551353823E-4 6 3.699070275676911E-4 7 0.0 8 0.0 9 0.001997497948865532 10-11 4.808791358379985E-4 12-13 9.98748974432766E-4 14-15 0.0021084700571358393 16-17 0.0025153677874602998 18-19 0.0 20-21 0.0 22-23 0.001368656002000457 24-25 0.0011467117854598425 26-27 0.004734809952866447 28-29 0.005067726277677369 30-31 0.0012206931909733807 32-33 2.959256220541529E-4 34-35 0.008655824445083973 36-37 0.011393136449084887 38-39 0.008877768661624586 40-41 0.001553609515784303 42-43 4.068977303244603E-4 44-45 8.137954606489206E-4 46-47 1.4796281102707646E-4 48-49 7.768047578921515E-4 50-51 8.507861634056896E-4 52-53 0.0017015723268113791 54-55 0.002663330598487376 56-57 0.001294674596486919 58-59 7.398140551353823E-4 60-61 0.0033661539508659894 62-63 0.003884023789460757 64-65 0.007989991795462129 66-67 0.0021824514626493778 68-69 0.002404395679189992 70-71 7.398140551353823E-5 72-73 8.137954606489206E-4 74-75 0.004956754169407061 76-77 0.0023674049764332234 78-79 0.0027003213012441454 80-81 0.0038100423839472182 82-83 0.003958005194974295 84-85 0.0045128657363258315 86-87 0.002663330598487376 88-89 0.008470870931300127 90-91 0.011393136449084887 92-93 5.918512441083058E-4 94-95 0.0017755537323249173 96-97 8.877768661624587E-4 98-99 0.0027003213012441454 100-101 0.004142958708758141 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1351691.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.86298324977224 #Duplication Level Percentage of deduplicated Percentage of total 1 81.70718858630053 51.36357627486592 2 11.309058210298028 14.218422736893285 3 3.1143501993202083 5.8733203324137335 4 1.2551510448517207 3.156101564337914 5 0.6955548024881176 2.186232494905459 6 0.41306137713172386 1.5579762259055645 7 0.27166286088638497 1.1954276511440218 8 0.19604626784689924 0.9859242601471992 9 0.14529323945531986 0.8220209830366427 >10 0.8011781270905177 9.355311427769701 >50 0.05867039869773746 2.529942984351334 >100 0.029129049917820254 3.4528317136549873 >500 0.0020048131340392717 0.8501736666079667 >1k 0.0015330923966182664 2.044108694574596 >5k 1.1793018435525127E-4 0.4086289893916213 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5512 0.40778550719062273 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4869 0.36021546344541766 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4332 0.32048744868464757 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2846 0.2105510800915298 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2447 0.18103249929162804 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2004 0.1482587366491306 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1912 0.14145244734188508 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1551 0.11474515995149778 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1500 0.11097210827030735 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1486 0.10993636859311781 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 7.398140551353823E-5 0.0 12-13 0.0 0.0 0.0 7.398140551353823E-5 0.0 14-15 0.0 0.0 0.0 7.398140551353823E-5 0.0 16-17 0.0 0.0 0.0 7.398140551353823E-5 0.0 18-19 0.0 0.0 0.0 7.398140551353823E-5 0.0 20-21 0.0 0.0 0.0 7.398140551353823E-5 3.6990702756769115E-5 22-23 0.0 0.0 0.0 7.398140551353823E-5 1.1097210827030735E-4 24-25 0.0 0.0 0.0 2.219442165406147E-4 1.4796281102707646E-4 26-27 0.0 0.0 0.0 3.32916324810922E-4 1.4796281102707646E-4 28-29 0.0 0.0 0.0 9.98748974432766E-4 1.4796281102707646E-4 30-31 0.0 3.6990702756769115E-5 0.0 0.0020714793543790706 1.4796281102707646E-4 32-33 0.0 7.398140551353823E-5 0.0 0.00377305168119045 1.4796281102707646E-4 34-35 0.0 7.398140551353823E-5 0.0 0.005622586819028905 1.4796281102707646E-4 36-37 0.0 7.398140551353823E-5 0.0 0.008174945309245974 1.4796281102707646E-4 38-39 0.0 7.398140551353823E-5 0.0 0.010579340988435966 1.4796281102707646E-4 40-41 0.0 7.398140551353823E-5 0.0 0.013649569317247803 1.4796281102707646E-4 42-43 0.0 7.398140551353823E-5 0.0 0.01845836067562779 1.4796281102707646E-4 44-45 0.0 7.398140551353823E-5 0.0 0.02715117582346853 1.4796281102707646E-4 46-47 0.0 7.398140551353823E-5 0.0 0.03810042383947219 1.4796281102707646E-4 48-49 0.0 7.398140551353823E-5 0.0 0.058667254572235816 1.4796281102707646E-4 50-51 0.0 7.398140551353823E-5 0.0 0.07812436422229636 1.4796281102707646E-4 52-53 0.0 7.398140551353823E-5 0.0 0.10028179517360107 2.219442165406147E-4 54-55 7.398140551353823E-5 7.398140551353823E-5 0.0 0.1332035206271256 2.959256220541529E-4 56-57 7.398140551353823E-5 7.398140551353823E-5 0.0 0.16823371613778593 2.959256220541529E-4 58-59 7.398140551353823E-5 7.398140551353823E-5 0.0 0.21432413177272025 3.32916324810922E-4 60-61 7.398140551353823E-5 7.398140551353823E-5 0.0 0.2640766269805747 3.699070275676911E-4 62-63 7.398140551353823E-5 7.398140551353823E-5 0.0 0.3363564601673016 3.699070275676911E-4 64-65 7.398140551353823E-5 7.398140551353823E-5 0.0 0.4025698181019183 4.438884330812294E-4 66-67 7.398140551353823E-5 7.398140551353823E-5 0.0 0.4915694489347048 4.438884330812294E-4 68-69 7.398140551353823E-5 7.398140551353823E-5 0.0 0.6130839074906913 4.438884330812294E-4 70-71 7.398140551353823E-5 7.398140551353823E-5 0.0 0.6836991590533635 4.438884330812294E-4 72-73 7.398140551353823E-5 7.398140551353823E-5 0.0 0.7851276660124245 4.438884330812294E-4 74-75 7.398140551353823E-5 7.398140551353823E-5 0.0 0.914817069877657 4.438884330812294E-4 76-77 7.398140551353823E-5 7.398140551353823E-5 0.0 1.0177621956497453 4.438884330812294E-4 78-79 7.398140551353823E-5 7.398140551353823E-5 0.0 1.1072427056183698 4.438884330812294E-4 80-81 7.398140551353823E-5 7.398140551353823E-5 0.0 1.2643422202263683 4.8087913583799854E-4 82-83 7.398140551353823E-5 7.398140551353823E-5 0.0 1.4559170698036756 5.178698385947676E-4 84-85 7.398140551353823E-5 7.398140551353823E-5 0.0 1.6789340167242366 5.178698385947676E-4 86-87 7.398140551353823E-5 7.398140551353823E-5 0.0 1.9924302225878547 5.178698385947676E-4 88-89 7.398140551353823E-5 7.398140551353823E-5 0.0 2.341215558881431 5.178698385947676E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGTCGT 145 2.3342593E-5 22.93164 9 GTACATG 5590 0.0 22.18433 1 GAGTACT 5705 0.0 22.147007 12-13 TACATGG 5700 0.0 21.498184 2 GTACTTT 5975 0.0 20.946703 14-15 ACATGGG 5610 0.0 20.91178 3 TAAGGTG 685 0.0 20.801163 5 AGTACTT 5875 0.0 20.172129 12-13 AGAGTAC 9105 0.0 19.511774 10-11 GTATAGG 390 0.0 19.492744 1 GTAAGGT 660 0.0 19.430176 4 GTATCAA 13005 0.0 18.888514 1 CATGGGG 2595 0.0 18.668943 4 GTACCTG 1125 0.0 18.583082 1 ACTTTTT 7160 0.0 17.645483 16-17 GGACCGC 220 2.9180641E-5 17.272545 6 TACTTTT 6835 0.0 17.268747 14-15 TACCTGG 1135 0.0 17.15714 2 CAACGCA 14575 0.0 16.815044 5 ATCAACG 14575 0.0 16.815044 3 >>END_MODULE