FastQCFastQC Report
Thu 26 May 2016
SRR936508_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936508_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1441076
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT52170.3620211564136798No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40080.2781255117703716No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31910.22143176348783825No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT31660.21969694866891132No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT22130.15356580777141524No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC20140.13975668181275658No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17870.12400456325689971No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT16610.11526109656950778No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG60950.026.2765221
TACATGG61400.025.4647392
ACATGGG60900.024.5041223
GTGTAGC8450.024.1837371
GGTTACG500.001656434323.74461482-83
GAGTACT44700.021.85125212-13
CATGGGG30100.021.789864
TAGCCCT9400.021.7411464
GTAGCCC7300.020.8331013
TACACCG1200.004745485719.8050485
GTACTTT50350.019.53709214-15
AGTACTT47950.019.37894612-13
AGAGTAC79500.019.04604510-11
ACCGTCC1759.8717726E-519.0168088
CATGGGA31650.018.9207654
GTATCAA122750.018.3900871
GTATAGG4253.6379788E-1217.8913351
GTACCTG12850.017.7521021
ATGGGGG15700.017.5596335
TATGACG1901.8579522E-417.5100064
Produced by FastQC (version 0.11.5)