##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936508_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1441076 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.391746167447103 31.0 31.0 34.0 30.0 34.0 2 31.44826990387738 31.0 31.0 34.0 28.0 34.0 3 31.401582567470417 31.0 31.0 34.0 28.0 34.0 4 35.06531716578446 37.0 35.0 37.0 32.0 37.0 5 35.10456075876637 37.0 35.0 37.0 32.0 37.0 6 35.1654097355032 37.0 35.0 37.0 32.0 37.0 7 35.033157862597115 37.0 35.0 37.0 32.0 37.0 8 35.016418981372254 37.0 35.0 37.0 32.0 37.0 9 36.51354474018025 39.0 37.0 39.0 32.0 39.0 10-11 36.401416719173724 39.0 37.0 39.0 32.0 39.0 12-13 36.31365660103978 39.0 36.0 39.0 32.0 39.0 14-15 37.30893859865823 40.0 37.0 41.0 32.0 41.0 16-17 37.23389536707294 39.5 37.0 41.0 31.5 41.0 18-19 37.11830951316933 39.0 36.0 41.0 31.0 41.0 20-21 37.09801877208419 39.0 36.0 41.0 31.0 41.0 22-23 37.09964498749545 39.0 36.5 41.0 31.0 41.0 24-25 37.21223099961418 39.0 37.0 41.0 31.0 41.0 26-27 37.14285887767196 39.0 36.0 41.0 31.0 41.0 28-29 37.03470948097116 39.0 36.0 41.0 30.5 41.0 30-31 36.85771222336643 39.0 36.0 41.0 30.0 41.0 32-33 36.70532227307928 39.0 36.0 41.0 30.0 41.0 34-35 36.50892145868781 39.0 35.0 41.0 30.0 41.0 36-37 36.31668697556548 39.0 35.0 40.0 30.0 41.0 38-39 36.135683336617916 39.0 35.0 40.0 30.0 41.0 40-41 35.92359424485593 38.5 35.0 40.0 28.5 41.0 42-43 35.658175210745306 38.0 35.0 40.0 27.5 41.0 44-45 35.53297640096706 38.0 35.0 40.0 27.0 41.0 46-47 35.41342371949849 38.0 35.0 40.0 27.0 41.0 48-49 35.19839654535916 38.0 34.5 40.0 26.0 41.0 50-51 34.21776297710878 37.5 33.0 39.5 25.0 40.5 52-53 34.33200504345365 38.0 33.5 39.5 24.5 40.5 54-55 34.79475232395794 38.0 34.0 40.0 26.0 41.0 56-57 34.646414901087795 38.0 34.0 40.0 24.5 41.0 58-59 34.43025975035321 38.0 34.0 40.0 24.0 41.0 60-61 34.14142661455746 38.0 33.5 40.0 23.0 41.0 62-63 33.8233483175072 37.0 33.0 40.0 23.0 41.0 64-65 33.47992576380427 36.5 33.0 39.5 22.0 41.0 66-67 33.11434858397475 36.0 32.0 39.0 22.0 41.0 68-69 32.68017752013079 35.5 32.0 39.0 20.5 40.5 70-71 32.283488865264566 35.0 31.5 38.5 20.0 40.0 72-73 31.86539675908835 35.0 31.0 37.5 18.5 40.0 74-75 31.469560939187108 35.0 31.0 37.0 18.0 39.0 76-77 31.004656936899927 35.0 30.5 36.5 14.0 39.0 78-79 30.53466784541551 34.0 30.0 36.0 9.5 38.5 80-81 30.08503784672009 34.0 30.0 35.5 7.0 37.0 82-83 29.683681152139094 34.0 29.0 35.0 6.5 37.0 84-85 29.315720336748374 34.0 29.0 35.0 2.0 36.5 86-87 28.99775723140209 34.0 29.0 35.0 2.0 36.0 88-89 28.65000596776298 34.0 29.0 35.0 2.0 36.0 90-91 28.296224487813273 34.0 29.0 35.0 2.0 35.0 92-93 27.884899547282725 34.0 27.0 35.0 2.0 35.0 94-95 27.40793996985586 33.5 26.5 35.0 2.0 35.0 96-97 26.827603818258027 33.0 25.5 35.0 2.0 35.0 98-99 26.116291576571953 33.0 24.5 35.0 2.0 35.0 100-101 23.541781627062 30.5 10.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5055907391693744 1101 2 0.5066181136640466 1101 3 0.5972953082566264 1101 4 0.6580485386624488 1101 5 0.5107645483359491 1101 6 0.5742073400549188 1101 7 0.5452397749255127 1101 8 0.5136852108580925 1101 9 0.7722649125737462 1101 10-11 0.7888522339190231 1101 12-13 0.7674129959473746 1101 14-15 0.9343000365975271 1101 16-17 1.026742060044512 1101 18-19 1.1779946581618717 1101 20-21 0.9561217064856038 1101 22-23 0.814481708913668 1101 24-25 0.7858388496777167 1101 26-27 0.7344286261911321 1101 28-29 0.6721090374334793 1101 30-31 0.6823381458638451 1101 32-33 0.6322053701510129 1101 34-35 0.7631748016527169 1101 36-37 0.6334129531705202 1101 38-39 0.7382003435720534 1101 40-41 0.696166241833275 1101 42-43 0.7684677318191859 1101 44-45 0.7250771039409969 1101 46-47 0.9257179783173299 1101 48-49 0.8639168864601956 1101 50-51 0.7226740225274924 1101 52-53 0.7478787462627565 1101 54-55 0.8919463978680469 1101 56-57 1.0526083620889821 1101 58-59 0.8816213483843001 1101 60-61 0.7546903266966254 1101 62-63 0.8068157384524568 1101 64-65 0.8632057375295545 1101 66-67 0.7983400749577783 1101 68-69 0.8106826958891986 1101 70-71 0.7319155682020622 1101 72-73 0.8256650015846425 1101 74-75 0.8366233192435146 1101 76-77 0.9216469342397886 1101 78-79 0.8468632229005486 1101 80-81 0.7545573524336469 1101 82-83 0.7069319989322018 1101 84-85 0.6804907669116318 1101 86-87 0.7920834352167141 1101 88-89 0.8481128704995271 1101 90-91 0.9892744851431807 1101 92-93 1.0691986083537905 1101 94-95 1.1069511242260113 1101 96-97 1.124550767905781 1101 98-99 1.2166681294525077 1101 100-101 1.645047484447712 1102 1 0.4247849456676711 1102 2 0.46783675488652676 1102 3 0.4637348786411266 1102 4 0.4444658564842783 1102 5 0.40729287432032635 1102 6 0.41148038252479324 1102 7 0.5092262745151288 1102 8 0.3890833965690419 1102 9 0.6367201655301429 1102 10-11 0.6159000671345041 1102 12-13 0.5654192760279955 1102 14-15 0.7823342303241105 1102 16-17 0.5613569398787206 1102 18-19 0.6498790545317306 1102 20-21 0.7091856453850909 1102 22-23 0.4977278406700023 1102 24-25 0.43174337913199423 1102 26-27 0.4025130448918972 1102 28-29 0.3438155700101788 1102 30-31 0.18696064563368253 1102 32-33 0.310602167035654 1102 34-35 -0.05027291559870406 1102 36-37 0.19696184514569381 1102 38-39 0.20093180646257025 1102 40-41 0.5332790501811218 1102 42-43 0.3918107875896766 1102 44-45 0.4085287193085847 1102 46-47 0.4917714589041182 1102 48-49 0.5032929528104475 1102 50-51 0.37312916187045175 1102 52-53 0.4369894860625152 1102 54-55 0.5741259345211134 1102 56-57 0.5266518055163516 1102 58-59 0.5297094105504385 1102 60-61 0.5429001903017436 1102 62-63 0.5583741034375862 1102 64-65 0.7388685641926642 1102 66-67 0.5583832088864682 1102 68-69 0.5982181266310391 1102 70-71 0.2995288829132434 1102 72-73 0.49648143165459047 1102 74-75 0.5623745880901154 1102 76-77 0.5841078796211079 1102 78-79 0.43446819290072725 1102 80-81 0.6558051413790587 1102 82-83 0.4953820178351265 1102 84-85 0.6103515120982408 1102 86-87 0.49401545128713664 1102 88-89 0.3935707816185605 1102 90-91 0.6009022326784539 1102 92-93 0.505166663389705 1102 94-95 0.6507894562240608 1102 96-97 0.5715107270049415 1102 98-99 0.5626649208523631 1102 100-101 1.020142243930028 1103 1 0.33222807198728077 1103 2 0.45167778568584893 1103 3 0.5687061754243175 1103 4 0.46737169989628313 1103 5 0.45349039812693803 1103 6 0.4357648385673798 1103 7 0.4148405844487755 1103 8 0.38285472942671817 1103 9 0.5261391408547595 1103 10-11 0.4623462212380147 1103 12-13 0.45543500770364886 1103 14-15 0.7867695373536208 1103 16-17 0.8714460496753418 1103 18-19 0.8760055276035033 1103 20-21 0.9130928539545238 1103 22-23 0.6418955928441008 1103 24-25 0.5801422465677177 1103 26-27 0.726283366791229 1103 28-29 0.5518131597109104 1103 30-31 0.5956371047775022 1103 32-33 0.8359535841592489 1103 34-35 0.7527393039058197 1103 36-37 0.609157384855628 1103 38-39 0.36916764049957607 1103 40-41 0.6290093512212493 1103 42-43 0.6814667199151074 1103 44-45 0.6721219271230723 1103 46-47 0.8747655757083592 1103 48-49 0.7150483619831078 1103 50-51 0.7104474166508652 1103 52-53 1.0204999189345187 1103 54-55 1.0749716120923054 1103 56-57 1.0685052994684128 1103 58-59 0.8912939386692642 1103 60-61 0.9494089074537797 1103 62-63 1.0045923918923663 1103 64-65 0.9220003967983317 1103 66-67 0.915326419937486 1103 68-69 1.1426492007270141 1103 70-71 1.0659458646861708 1103 72-73 0.961896473696644 1103 74-75 1.0730511083278316 1103 76-77 1.056157790416762 1103 78-79 1.0272428953771353 1103 80-81 0.9605465360506535 1103 82-83 0.8189408748848876 1103 84-85 0.8974867761438361 1103 86-87 0.9831038286844347 1103 88-89 0.858053596417534 1103 90-91 0.844673201882042 1103 92-93 1.0745073148978186 1103 94-95 1.2299744055997728 1103 96-97 1.1714970536114038 1103 98-99 1.1911173618076667 1103 100-101 1.5546535619360675 1104 1 0.4140156257452503 1104 2 0.3194729717953564 1104 3 0.4812746402752204 1104 4 0.467340555915591 1104 5 0.4899150137756152 1104 6 0.47137555818711974 1104 7 0.5620623337438886 1104 8 0.5807920289805963 1104 9 0.5432600251777089 1104 10-11 0.5467315290305166 1104 12-13 0.6161514438356548 1104 14-15 0.6113749431181574 1104 16-17 0.668047826128003 1104 18-19 0.5476586916243136 1104 20-21 0.6360114325155592 1104 22-23 0.5693948280863523 1104 24-25 0.5738155199108235 1104 26-27 0.37848919113086765 1104 28-29 0.30698271446272685 1104 30-31 0.43669933598764743 1104 32-33 0.4330549792711693 1104 34-35 0.5408103980007795 1104 36-37 0.40332993081376145 1104 38-39 0.31445770169404597 1104 40-41 0.34623446406626357 1104 42-43 0.5891839907057914 1104 44-45 0.4747078464449359 1104 46-47 0.5159353574651817 1104 48-49 0.6832614311047465 1104 50-51 0.6549647610797606 1104 52-53 0.5258701793397265 1104 54-55 0.6587163920151013 1104 56-57 0.5940634342069657 1104 58-59 0.45532111836726585 1104 60-61 0.44050830381421946 1104 62-63 0.5508308219035314 1104 64-65 0.6502200269185678 1104 66-67 0.663341510234261 1104 68-69 0.42363019459372 1104 70-71 0.532464701257787 1104 72-73 0.6946330191509311 1104 74-75 0.5701135388594523 1104 76-77 0.47095651032994823 1104 78-79 0.32911873193906516 1104 80-81 0.5313053141546362 1104 82-83 0.46430298248803226 1104 84-85 0.391412661774833 1104 86-87 0.43301565661463215 1104 88-89 0.6048765643420033 1104 90-91 0.728279148859766 1104 92-93 0.6473475490451825 1104 94-95 0.5686793835152315 1104 96-97 0.3080641010651419 1104 98-99 0.16494430647242808 1104 100-101 0.9816617604159639 1105 1 0.2605634630832192 1105 2 0.3203057825548221 1105 3 0.37310978740291745 1105 4 0.46716592363560494 1105 5 0.3090475676139519 1105 6 0.37245232494983327 1105 7 0.24891027400906296 1105 8 0.37756305166427495 1105 9 0.3326852468416348 1105 10-11 0.44170807274017676 1105 12-13 0.40440451246877984 1105 14-15 0.782541036938035 1105 16-17 0.6538457236611848 1105 18-19 0.4321007414897693 1105 20-21 0.4891665218558501 1105 22-23 0.5105024068627131 1105 24-25 0.3994888464176256 1105 26-27 0.3515607612602807 1105 28-29 0.38413677717688444 1105 30-31 0.34965175927512604 1105 32-33 0.3023384180453519 1105 34-35 0.12376701510891763 1105 36-37 0.1736924515513465 1105 38-39 0.05868656788073423 1105 40-41 0.20009899704398748 1105 42-43 -0.13053649180849902 1105 44-45 -0.007961553481500516 1105 46-47 0.4744228170153164 1105 48-49 0.5640186737139672 1105 50-51 0.6230732024337371 1105 52-53 0.677467866255796 1105 54-55 0.4451126697015937 1105 56-57 0.5122799623584839 1105 58-59 0.46515046823245143 1105 60-61 0.3393720694682898 1105 62-63 0.44417184170312396 1105 64-65 0.6043201811770871 1105 66-67 0.5520415448961273 1105 68-69 0.5144687470508771 1105 70-71 0.6023047142758315 1105 72-73 0.39747337349570344 1105 74-75 0.5896584630134782 1105 76-77 0.6419261428348335 1105 78-79 0.8524450402920429 1105 80-81 0.5595682918466842 1105 82-83 0.4439358313490871 1105 84-85 0.7039521855578954 1105 86-87 0.6754955540578571 1105 88-89 0.6888006045920996 1105 90-91 0.5374381489047444 1105 92-93 0.5140958430730258 1105 94-95 0.49813819543362214 1105 96-97 0.6124170752380849 1105 98-99 0.8023878486500919 1105 100-101 1.002328895414653 1106 1 0.1926387028176677 1106 2 0.12291076028888526 1106 3 0.09214921040103263 1106 4 0.28746753825735993 1106 5 0.25175718158001814 1106 6 0.1865044518246819 1106 7 0.33468876211112786 1106 8 0.38929023249644246 1106 9 0.4347670434156896 1106 10-11 0.49160152409662317 1106 12-13 0.6256910190392801 1106 14-15 0.3483075041674937 1106 16-17 0.26401428907932 1106 18-19 0.322099512082346 1106 20-21 0.09338953636284941 1106 22-23 -0.11524889767516555 1106 24-25 -0.3128093258633413 1106 26-27 -0.18194742112914497 1106 28-29 0.026970014889634797 1106 30-31 0.002849584589995402 1106 32-33 0.19245329933923472 1106 34-35 0.12698054951867022 1106 36-37 -0.20865325781674215 1106 38-39 0.03526977144328214 1106 40-41 0.16868763731937264 1106 42-43 0.08988942956406731 1106 44-45 -0.01943943780792523 1106 46-47 0.09059840371586603 1106 48-49 0.0269793599965098 1106 50-51 0.1349711342972526 1106 52-53 0.21419641309621795 1106 54-55 0.259007705627603 1106 56-57 0.3019898222060391 1106 58-59 0.3320432092646115 1106 60-61 0.4731964827677402 1106 62-63 0.14356465103570315 1106 64-65 -0.1815648555685314 1106 66-67 -0.04496211600599054 1106 68-69 0.19683084851796906 1106 70-71 -0.0413822785936091 1106 72-73 0.08412815589059264 1106 74-75 -0.0084482495510656 1106 76-77 0.03346508773006107 1106 78-79 0.09169797037974092 1106 80-81 0.20500377527700664 1106 82-83 0.21932992472941137 1106 84-85 0.36796196617697063 1106 86-87 0.6674941607294436 1106 88-89 0.5580804450423358 1106 90-91 0.7511057444570248 1106 92-93 0.6228710800754591 1106 94-95 0.801409102189897 1106 96-97 0.9200489633346862 1106 98-99 0.8641245915400191 1106 100-101 1.5201040777767574 1107 1 0.35055647831224945 1107 2 0.3183029242478348 1107 3 0.392897494949068 1107 4 0.44192229259498816 1107 5 0.4194849703194379 1107 6 0.3737833325030451 1107 7 0.4103547717061957 1107 8 0.3592407218220899 1107 9 0.41361384985029304 1107 10-11 0.558347246159407 1107 12-13 0.45103374125842066 1107 14-15 0.2804533458630587 1107 16-17 0.40031277944399335 1107 18-19 0.8037179574640945 1107 20-21 0.4531656933431236 1107 22-23 0.477677853411258 1107 24-25 0.36039734963321735 1107 26-27 0.38841595482141145 1107 28-29 0.3823368673349208 1107 30-31 0.36022117910568596 1107 32-33 0.41937033002733415 1107 34-35 0.40715939081034236 1107 36-37 0.4031068825340043 1107 38-39 0.4819170925683096 1107 40-41 0.4363152202891527 1107 42-43 0.45932958387719225 1107 44-45 0.6545803520482281 1107 46-47 0.549619790033546 1107 48-49 0.6207789164966826 1107 50-51 0.4571489772912898 1107 52-53 0.4593539959124655 1107 54-55 0.5196367083357956 1107 56-57 0.7117518319364677 1107 58-59 0.7233075723265969 1107 60-61 0.8191996731840803 1107 62-63 0.6891253756380991 1107 64-65 0.4965971174385757 1107 66-67 0.4874706500899961 1107 68-69 0.4958364989577646 1107 70-71 0.5397682578581993 1107 72-73 0.6648986122968559 1107 74-75 0.5691778513258754 1107 76-77 0.698925713098042 1107 78-79 0.7752767890483447 1107 80-81 0.7224482900138014 1107 82-83 0.7406433911252286 1107 84-85 0.6477579073732933 1107 86-87 0.7418244067349207 1107 88-89 0.8028712647148772 1107 90-91 0.8406080150671222 1107 92-93 1.0284938833741641 1107 94-95 1.0319141762428394 1107 96-97 1.1186572374234736 1107 98-99 1.1605663467014153 1107 100-101 1.6395476004827039 1108 1 0.16111991997560082 1108 2 0.048782807953543283 1108 3 0.24593590529777387 1108 4 0.20500499231746971 1108 5 0.1823006345310958 1108 6 0.14045534205625643 1108 7 0.33292198190002864 1108 8 0.38348578337899397 1108 9 0.03204197698138955 1108 10-11 0.12531749977458873 1108 12-13 0.07518174944446088 1108 14-15 0.44924802656791485 1108 16-17 0.4996934072255499 1108 18-19 0.417253589239742 1108 20-21 0.35917833411212996 1108 22-23 0.40890870104008314 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84-85 -4.427426099780419 2316 86-87 -5.058735703211891 2316 88-89 -6.551174487344504 2316 90-91 -5.517907391729469 2316 92-93 -5.188625832816673 2316 94-95 -4.719979189689685 2316 96-97 -6.1481453535330814 2316 98-99 -7.971280686121332 2316 100-101 -8.451792390405371 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3248.0 3 1223.0 4 1283.0 5 1387.0 6 1488.0 7 1614.0 8 2168.0 9 2676.0 10 3651.0 11 4466.0 12 5612.0 13 6657.0 14 7755.0 15 8920.0 16 10823.0 17 13033.0 18 10856.0 19 8657.0 20 8937.0 21 9809.0 22 10625.0 23 12235.0 24 14068.0 25 16268.0 26 19204.0 27 22757.0 28 27492.0 29 32711.0 30 39739.0 31 49127.0 32 61483.0 33 78216.0 34 103620.0 35 141083.0 36 197471.0 37 261836.0 38 208893.0 39 29977.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.24070025528983 18.62803243486498 14.463946908604017 22.66732040124117 2 15.413513218970456 20.207910627306735 40.13302615056445 24.245550003158364 3 19.958024456583974 24.278632102545807 29.49663355864188 26.26670988222834 4 12.750068887588641 16.420580071670855 37.78989181351696 33.039459227223546 5 13.8071483157875 37.4129851884809 34.105225018619386 14.674641477112216 6 31.728756191830115 36.06019125258224 17.629709122814813 14.581343432772828 7 28.62405123685587 32.713985197593445 20.532731069705488 18.1292324958452 8 26.31786883003535 34.057989630772454 19.795753852007643 19.828387687184552 9 26.09872290779797 17.444148917700815 20.740074583851275 35.71705359064993 10-11 25.084748469597486 26.431662389316624 27.205473897054738 21.278115244031152 12-13 26.344921330467574 23.84317882670488 27.544645584453814 22.267254258373736 14-15 22.880512244108782 25.41847547255741 26.31468050349658 25.386331779837228 16-17 22.19962201059259 28.739137317636082 26.764274585280912 22.296966086490418 18-19 22.080136136036057 27.448858729008197 28.93352467916941 21.537480455786337 20-21 22.955299222684197 26.229402380566512 28.8418206242888 21.973477772460484 22-23 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27.12487591655208 27.778280877391847 22.25459408014828 48-49 22.764669389658458 27.169753973111497 27.65089218923266 22.41468444799738 50-51 22.296349703447667 27.362825029613596 27.964118104646506 22.376707162292227 52-53 22.845156752934532 27.46969533476259 27.32362367360166 22.361524238701218 54-55 22.306935392541106 27.164673243252246 27.676340946184506 22.852050418022138 56-57 22.69154033788684 27.345690364083648 27.351623681918646 22.611145616110868 58-59 23.116804168174575 26.97602973460711 27.355207043371184 22.551959053847135 60-61 22.60912892556707 27.139934573303854 28.13053000620402 22.12040649492506 62-63 22.370649902743246 27.321480245740727 27.934617645283133 22.3732522062329 64-65 22.09795460372898 27.60735025576561 27.631221481605106 22.66347365890031 66-67 22.375303291000105 27.409769084216446 27.301203657152534 22.913723967630915 68-69 22.344284342245516 27.179075435246048 27.713625866050805 22.76301435645763 70-71 22.583565312219932 27.27811478279455 27.367910822955725 22.770409082029794 72-73 22.49945601683554 27.038305784822818 27.829970470476894 22.632267727864743 74-75 22.1956562702028 27.8877141648538 27.31600639479866 22.600623170144743 76-77 22.44032592467139 27.391006183795252 27.27342453430656 22.895243357226793 78-79 22.520145198120474 27.4184301667835 27.357283157156836 22.704141477939185 80-81 22.647195073920685 27.6033596999103 27.152427037915473 22.597018188253543 82-83 22.599648508590374 27.527294366248494 27.42313077180643 22.449926353354694 84-85 22.42953978623564 27.65956339441481 27.269875769070097 22.641021050279452 86-87 22.538692192445385 27.677885546194346 27.112396282440688 22.67102597891958 88-89 22.323090310788135 27.633434324789075 26.85483322530995 23.18864213911284 90-91 22.4223403129462 27.352899844377017 27.200781411294027 23.023978431382755 92-93 22.734291611320007 27.380531022584215 27.352042239738832 22.53313512635695 94-95 22.35430677299132 27.321340578955773 27.413264208429627 22.911088439623285 96-97 22.10526973273968 27.256673526969454 27.719995544446746 22.91806119584412 98-99 22.166463296976662 27.509706911654263 27.055109715518427 23.268720075850645 100-101 22.008843918920796 27.316305793315216 27.285419402483686 23.38943088528031 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 117.0 1 95.0 2 105.0 3 131.0 4 117.5 5 129.5 6 208.0 7 398.5 8 689.5 9 870.0 10 845.5 11 807.5 12 864.5 13 997.5 14 1153.5 15 1242.0 16 1328.5 17 1452.0 18 1578.0 19 1745.5 20 1990.5 21 2322.5 22 2733.0 23 3328.5 24 4163.5 25 5271.5 26 6657.5 27 8412.5 28 10545.5 29 13041.0 30 15879.0 31 19335.0 32 23301.5 33 26016.5 34 28378.0 35 31054.0 36 33963.5 37 37334.5 38 39802.5 39 41258.5 40 42698.5 41 45378.5 42 47779.0 43 50001.5 44 52102.5 45 54128.5 46 55653.5 47 59350.0 48 67780.5 49 66788.0 50 57387.5 51 52185.0 52 49276.5 53 48211.5 54 46692.0 55 42645.0 56 38165.5 57 33826.5 58 30436.0 59 26533.5 60 21928.0 61 18063.5 62 15038.0 63 11721.0 64 8565.0 65 6803.0 66 5308.5 67 4151.0 68 3124.5 69 2218.0 70 1713.0 71 1297.5 72 1009.5 73 882.0 74 721.5 75 603.0 76 471.5 77 294.5 78 174.5 79 110.5 80 77.0 81 55.5 82 40.0 83 20.5 84 10.5 85 9.5 86 7.5 87 4.0 88 4.5 89 5.5 90 3.0 91 1.5 92 2.5 93 3.5 94 2.5 95 3.0 96 2.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07938512611409808 2 0.03171241488998498 3 0.01644604448342766 4 0.022413807460536433 5 0.02588343709839037 6 0.032475733410312846 7 0.08153629648956752 8 0.059191881621788164 9 0.0753603557341875 10-11 0.06467389644959738 12-13 0.043023407509388815 14-15 0.04642364455448567 16-17 0.05717949643183288 18-19 0.05218322975332321 20-21 0.0197421926393889 22-23 0.014017303736929906 24-25 0.02366287413016385 26-27 0.025536474134604976 28-29 0.007320918535871806 30-31 0.0011449777804917992 32-33 0.009749659282369563 34-35 0.011900829657839004 36-37 0.004753392603859893 38-39 0.010200711135290575 40-41 0.004371733343695961 42-43 0.0024287407464977555 44-45 4.8574814929955117E-4 46-47 6.59229631192248E-4 48-49 9.714962985991023E-4 50-51 0.001040888891356181 52-53 1.7348148189269682E-4 54-55 2.7757037102831494E-4 56-57 0.004232948158181803 58-59 0.004267644454560343 60-61 0.005135051864023827 62-63 0.0031226666740685432 64-65 7.286222239493268E-4 66-67 6.59229631192248E-4 68-69 0.003608414823368095 70-71 0.0022552592646050587 72-73 0.021823970422101262 74-75 0.015092888924664625 76-77 0.02602222228390453 78-79 0.01984628152852452 80-81 0.01203961484335316 82-83 0.0051003555676452875 84-85 0.003469629637853937 86-87 0.001596029633412811 88-89 0.002081777782712362 90-91 0.006488207422786862 92-93 0.010964029655618442 94-95 0.01429487410795822 96-97 0.012317185214381476 98-99 0.024287407464977557 100-101 0.021650488940208566 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1441076.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.4951176701511 #Duplication Level Percentage of deduplicated Percentage of total 1 82.5559975367801 53.24458775511337 2 10.811091966825332 13.945252970864502 3 2.9702043959908764 5.7469104607149495 4 1.217497498679253 3.1409057776173226 5 0.647249751854739 2.0872224453923742 6 0.3754475126343993 1.4528718903792674 7 0.2463414298525901 1.112147365376325 8 0.1855924806493946 0.9575847102542356 9 0.13630406178453364 0.7911851853341738 >10 0.7691847653439663 9.083811752031762 >50 0.05706587557813656 2.534811609716953 >100 0.024789413084919252 3.077158559311165 >500 0.0018322095336762636 0.7856124542113205 >1k 0.0012933243767126567 1.6772985772535096 >5k 1.077770313927214E-4 0.36263848642899127 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5217 0.3620211564136798 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4008 0.2781255117703716 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3191 0.22143176348783825 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3166 0.21969694866891132 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2213 0.15356580777141524 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2014 0.13975668181275658 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1787 0.12400456325689971 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1661 0.11526109656950778 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 6.939259275707873E-5 0.0 5 0.0 0.0 0.0 6.939259275707873E-5 0.0 6 0.0 0.0 0.0 6.939259275707873E-5 0.0 7 0.0 0.0 0.0 6.939259275707873E-5 0.0 8 0.0 0.0 0.0 6.939259275707873E-5 0.0 9 0.0 0.0 0.0 6.939259275707873E-5 0.0 10-11 0.0 0.0 0.0 6.939259275707873E-5 0.0 12-13 0.0 0.0 0.0 6.939259275707873E-5 0.0 14-15 0.0 0.0 0.0 6.939259275707873E-5 0.0 16-17 0.0 0.0 0.0 6.939259275707873E-5 3.469629637853937E-5 18-19 0.0 0.0 0.0 1.3878518551415747E-4 6.939259275707873E-5 20-21 0.0 0.0 0.0 1.3878518551415747E-4 6.939259275707873E-5 22-23 0.0 0.0 0.0 2.081777782712362E-4 6.939259275707873E-5 24-25 0.0 0.0 0.0 2.4287407464977558E-4 6.939259275707873E-5 26-27 0.0 0.0 0.0 3.1226666740685435E-4 6.939259275707873E-5 28-29 0.0 0.0 0.0 6.939259275707874E-4 6.939259275707873E-5 30-31 0.0 6.939259275707873E-5 0.0 0.001491940744277193 6.939259275707873E-5 32-33 0.0 6.939259275707873E-5 0.0 0.002532829635633374 6.939259275707873E-5 34-35 0.0 6.939259275707873E-5 0.0 0.0037818963052607913 6.939259275707873E-5 36-37 0.0 6.939259275707873E-5 0.0 0.0054126222350521416 6.939259275707873E-5 38-39 0.0 6.939259275707873E-5 0.0 0.007806666685171358 6.939259275707873E-5 40-41 0.0 6.939259275707873E-5 0.0 0.010582370395454507 1.3878518551415747E-4 42-43 0.0 6.939259275707873E-5 0.0 0.016064385223263727 2.081777782712362E-4 44-45 0.0 6.939259275707873E-5 0.0 0.024877244503412728 2.7757037102831494E-4 46-47 0.0 6.939259275707873E-5 0.0 0.034141155636482734 2.7757037102831494E-4 48-49 0.0 6.939259275707873E-5 0.0 0.05540998531652737 2.7757037102831494E-4 50-51 0.0 6.939259275707873E-5 0.0 0.07539505203056604 2.7757037102831494E-4 52-53 0.0 6.939259275707873E-5 0.0 0.09399226688946316 2.7757037102831494E-4 54-55 0.0 6.939259275707873E-5 0.0 0.1205696299154243 2.7757037102831494E-4 56-57 0.0 6.939259275707873E-5 0.0 0.1535311114750367 2.7757037102831494E-4 58-59 0.0 6.939259275707873E-5 0.0 0.19377881527414237 2.7757037102831494E-4 60-61 0.0 6.939259275707873E-5 0.0 0.24197197094393358 2.7757037102831494E-4 62-63 0.0 6.939259275707873E-5 0.0 0.31185031185031187 3.469629637853937E-4 64-65 0.0 6.939259275707873E-5 0.0 0.374581215702711 3.469629637853937E-4 66-67 0.0 6.939259275707873E-5 0.0 0.45937896405186124 3.469629637853937E-4 68-69 0.0 6.939259275707873E-5 0.0 0.5740849198793123 3.469629637853937E-4 70-71 0.0 6.939259275707873E-5 0.0 0.6438938681929336 3.469629637853937E-4 72-73 0.0 6.939259275707873E-5 0.0 0.7417027276840361 3.469629637853937E-4 74-75 0.0 6.939259275707873E-5 0.0 0.8651521501988793 3.469629637853937E-4 76-77 0.0 6.939259275707873E-5 0.0 0.9603587874615912 3.469629637853937E-4 78-79 0.0 6.939259275707873E-5 0.0 1.0506385506385505 3.469629637853937E-4 80-81 0.0 6.939259275707873E-5 0.0 1.195391499129817 3.469629637853937E-4 82-83 0.0 6.939259275707873E-5 0.0 1.3763326847438997 4.163555565424724E-4 84-85 0.0 6.939259275707873E-5 0.0 1.5910333667343013 4.163555565424724E-4 86-87 0.0 6.939259275707873E-5 0.0 1.8923013081891586 4.163555565424724E-4 88-89 0.0 6.939259275707873E-5 0.0 2.2345802719634493 4.163555565424724E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 6095 0.0 26.276522 1 TACATGG 6140 0.0 25.464739 2 ACATGGG 6090 0.0 24.504122 3 GTGTAGC 845 0.0 24.183737 1 GGTTACG 50 0.0016564343 23.744614 82-83 GAGTACT 4470 0.0 21.851252 12-13 CATGGGG 3010 0.0 21.78986 4 TAGCCCT 940 0.0 21.741146 4 GTAGCCC 730 0.0 20.833101 3 TACACCG 120 0.0047454857 19.805048 5 GTACTTT 5035 0.0 19.537092 14-15 AGTACTT 4795 0.0 19.378946 12-13 AGAGTAC 7950 0.0 19.046045 10-11 ACCGTCC 175 9.8717726E-5 19.016808 8 CATGGGA 3165 0.0 18.920765 4 GTATCAA 12275 0.0 18.390087 1 GTATAGG 425 3.6379788E-12 17.891335 1 GTACCTG 1285 0.0 17.752102 1 ATGGGGG 1570 0.0 17.559633 5 TATGACG 190 1.8579522E-4 17.510006 4 >>END_MODULE