FastQCFastQC Report
Thu 26 May 2016
SRR936508_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936508_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1441076
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT60140.4173270528410715No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48850.33898281561832966No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44840.31115638592274103No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT29450.20436118566959688No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25900.17972681524083392No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT22080.15321884480762987No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC21130.14662654849570736No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC17180.11921647435666127No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT15830.10984847433445566No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT15740.10922394099964193No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG61600.023.1446671
TACATGG61300.022.7065772
ACCGTCG1050.00219898122.6146378
GAGTACT59250.022.44287912-13
ACATGGG59450.022.2091523
GTACTTT61500.021.2728314-15
AGTACTT61150.020.46411912-13
GTAAGGT6850.020.1048684
AGAGTAC94600.019.8798410-11
GTACACG2401.406388E-719.801551
TAAGGTG9000.019.523295
ATACCGT1507.7361055E-418.9949766
ACTTTTT70050.018.87973216-17
TACTTTT67350.018.57897614-15
GTATAGG5000.018.0590111
CATGGGG27550.017.9269144
GTATCAA142650.017.3903831
GGTAAGG7400.017.3289033
GTCTTAC4950.017.2813511
GGTATCA118550.017.0772691
Produced by FastQC (version 0.11.5)