##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936508_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1441076 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.894102045971206 31.0 31.0 34.0 30.0 34.0 2 32.057962244878134 31.0 31.0 34.0 30.0 34.0 3 32.139903100183474 33.0 31.0 34.0 30.0 34.0 4 35.839898103916795 37.0 35.0 37.0 35.0 37.0 5 35.67436970707999 37.0 35.0 37.0 33.0 37.0 6 35.75978990698617 37.0 35.0 37.0 33.0 37.0 7 35.70497184048586 37.0 35.0 37.0 33.0 37.0 8 35.67530928278592 37.0 35.0 37.0 33.0 37.0 9 37.313788446966015 39.0 37.0 39.0 34.0 39.0 10-11 37.198130424765935 39.0 37.0 39.0 33.0 39.0 12-13 37.10138986424033 39.0 37.0 39.0 33.0 39.0 14-15 38.23087297269541 40.0 38.0 41.0 33.0 41.0 16-17 38.13521181395013 40.0 37.0 41.0 33.0 41.0 18-19 38.284401377859325 40.0 38.0 41.0 33.5 41.0 20-21 38.30164925375206 40.0 38.0 41.0 34.0 41.0 22-23 38.2254943528308 40.0 38.0 41.0 33.0 41.0 24-25 38.0853653797579 40.0 37.0 41.0 33.0 41.0 26-27 37.89464608389842 40.0 37.0 41.0 33.0 41.0 28-29 37.81726189319647 40.0 37.0 41.0 33.0 41.0 30-31 37.79311500573183 40.0 37.0 41.0 33.0 41.0 32-33 37.6413131576683 40.0 37.0 41.0 33.0 41.0 34-35 37.46127928020451 40.0 36.0 41.0 32.0 41.0 36-37 37.258225104019495 39.0 36.0 41.0 31.5 41.0 38-39 37.093204660961675 39.0 36.0 41.0 31.0 41.0 40-41 36.910968262603774 39.0 36.0 41.0 31.0 41.0 42-43 36.643998651008 39.0 35.0 41.0 30.0 41.0 44-45 36.37968295912221 39.0 35.0 40.0 30.0 41.0 46-47 36.01875994048891 39.0 35.0 40.0 29.5 41.0 48-49 35.65079114495002 38.5 35.0 40.0 28.0 41.0 50-51 35.35738954781011 38.0 35.0 40.0 27.0 41.0 52-53 35.00630778668162 38.0 34.0 40.0 26.0 41.0 54-55 34.831228193377726 38.0 34.0 40.0 26.0 41.0 56-57 34.538110411942185 38.0 34.0 40.0 26.0 41.0 58-59 34.16790717491652 37.5 33.0 40.0 25.0 41.0 60-61 33.750664781038616 37.0 33.0 40.0 23.0 41.0 62-63 33.43901466681841 36.5 32.5 39.0 23.0 40.5 64-65 33.027882637695726 36.0 32.0 39.0 22.0 40.0 66-67 32.61079394841077 35.5 31.0 39.0 22.0 40.0 68-69 32.25359627111963 35.0 31.0 38.0 21.0 40.0 70-71 31.877234094523814 35.0 31.0 38.0 20.0 40.0 72-73 31.419265534919738 35.0 30.5 37.0 18.5 39.0 74-75 30.928056882496136 34.0 30.0 36.5 17.5 39.0 76-77 29.430045674204553 32.5 28.5 35.0 14.0 37.0 78-79 30.205575209079882 34.0 29.5 36.0 14.0 37.5 80-81 29.960018069831154 34.0 29.0 35.0 9.0 37.0 82-83 29.660001276823706 34.0 29.0 35.0 7.5 37.0 84-85 29.28270403504048 34.0 29.0 35.0 6.5 36.0 86-87 28.911799585865005 34.0 29.0 35.0 2.0 36.0 88-89 28.4511736369213 33.0 28.5 35.0 2.0 35.0 90-91 27.97462174097688 33.0 27.0 35.0 2.0 35.0 92-93 27.359356133888845 33.0 26.0 34.5 2.0 35.0 94-95 26.82406757173112 32.0 25.5 34.0 2.0 35.0 96-97 26.10147487016646 32.0 24.5 34.0 2.0 35.0 98-99 25.227503962317044 32.0 21.5 34.0 2.0 35.0 100-101 21.983253485589934 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.31891247343527596 1101 2 0.3572756474339087 1101 3 0.32430687983978856 1101 4 0.28687944353503525 1101 5 0.25565368041146996 1101 6 0.29676202974666666 1101 7 0.30630011847951977 1101 8 0.2766589794759682 1101 9 0.319568287406895 1101 10-11 0.45650919786168487 1101 12-13 0.4053381227761079 1101 14-15 0.5342731183287626 1101 16-17 0.5002509313923298 1101 18-19 0.5165816726356809 1101 20-21 0.372026163624561 1101 22-23 0.390260984364879 1101 24-25 0.33361979692094934 1101 26-27 0.3291725331971094 1101 28-29 0.4067779097670581 1101 30-31 0.4562861541703498 1101 32-33 0.566455744628847 1101 34-35 0.6125717644422934 1101 36-37 0.4870836742346185 1101 38-39 0.3332754460736993 1101 40-41 0.43938405843253037 1101 42-43 0.5787625751208765 1101 44-45 0.6615925602599972 1101 46-47 0.716156752666322 1101 48-49 0.7380446060110373 1101 50-51 0.5931028205891664 1101 52-53 0.8916062600460393 1101 54-55 0.8685957697309377 1101 56-57 0.8686061755941452 1101 58-59 0.7653968579744586 1101 60-61 0.9169282106732481 1101 62-63 0.9354624402292373 1101 64-65 0.8100791931175451 1101 66-67 0.5310641048248996 1101 68-69 0.6519023935979718 1101 70-71 0.774010542063877 1101 72-73 0.6811620845744955 1101 74-75 0.5017606873207896 1101 76-77 0.4553741876234092 1101 78-79 0.5297688579444078 1101 80-81 0.619561828069159 1101 82-83 0.9130611298600542 1101 84-85 0.7211728427998878 1101 86-87 0.5999779708736135 1101 88-89 0.7182033635855483 1101 90-91 0.581241796115652 1101 92-93 0.6999380560174089 1101 94-95 0.6296395170209266 1101 96-97 0.9854979351626412 1101 98-99 0.894464404616695 1101 100-101 1.2596305150509615 1102 1 0.30295213627761086 1102 2 0.20784263724591767 1102 3 0.22246810514110393 1102 4 0.17358099083250522 1102 5 0.19681289005372093 1102 6 0.2452634097581452 1102 7 0.25130024580492005 1102 8 0.20148175230981735 1102 9 0.26104992414555994 1102 10-11 0.3118491273038231 1102 12-13 0.29067356644636533 1102 14-15 0.4807423849148549 1102 16-17 0.44830334244574743 1102 18-19 0.3435642760679798 1102 20-21 0.25519100300846986 1102 22-23 0.3065603988639154 1102 24-25 0.3933477515069157 1102 26-27 0.28853222354173624 1102 28-29 0.36608960822916004 1102 30-31 0.5158190070625039 1102 32-33 0.548839001926062 1102 34-35 0.5799709765918806 1102 36-37 0.5152901686136957 1102 38-39 0.43824505447953044 1102 40-41 0.5350334473685407 1102 42-43 0.474808435772033 1102 44-45 0.6190361012382084 1102 46-47 0.5067938302153294 1102 48-49 0.5901040454266706 1102 50-51 0.4948524675060355 1102 52-53 0.6491359905275473 1102 54-55 0.6266304923476511 1102 56-57 0.5590601004486544 1102 58-59 0.5875350236659429 1102 60-61 0.7744677354164935 1102 62-63 0.7649435598308827 1102 64-65 0.5246446315184983 1102 66-67 0.3548451599802931 1102 68-69 0.3691951421224715 1102 70-71 0.4033657074595709 1102 72-73 0.6186844889482224 1102 74-75 0.730298227746605 1102 76-77 0.7186529538598485 1102 78-79 0.753922878586792 1102 80-81 0.70591195725547 1102 82-83 0.5738815364002683 1102 84-85 0.6296832727092152 1102 86-87 0.7163529970732654 1102 88-89 0.49119686215460945 1102 90-91 0.4968896963238798 1102 92-93 0.8054114812470345 1102 94-95 0.6954506875723254 1102 96-97 1.072503692229695 1102 98-99 1.0111861476818689 1102 100-101 1.664559183442723 1103 1 0.26203777899620206 1103 2 0.27948030977250227 1103 3 0.16573734080568414 1103 4 0.20772883658207775 1103 5 0.2734806258633924 1103 6 0.1351431348065617 1103 7 0.13402325848990415 1103 8 0.09993353141469186 1103 9 0.2542383195073725 1103 10-11 0.1784006247222365 1103 12-13 0.2185847810291861 1103 14-15 0.2720751262193417 1103 16-17 0.304621068473125 1103 18-19 0.21264680590761742 1103 20-21 0.09952323592454348 1103 22-23 0.137624348889112 1103 24-25 0.21391628102799132 1103 26-27 0.21537974993029252 1103 28-29 0.1768956620514146 1103 30-31 0.25260672690048125 1103 32-33 0.301522769062359 1103 34-35 0.30534650398121244 1103 36-37 0.3463712103808234 1103 38-39 0.2859998178025407 1103 40-41 0.28957639600677254 1103 42-43 0.11301117001436012 1103 44-45 0.12783362082643634 1103 46-47 0.3395078606444528 1103 48-49 0.2326371724954086 1103 50-51 0.27625727050370585 1103 52-53 0.26703537180649306 1103 54-55 0.14745114324482245 1103 56-57 0.17034934145166147 1103 58-59 0.3172929464156269 1103 60-61 0.3418606556533348 1103 62-63 0.4548100842360725 1103 64-65 0.37196319176834436 1103 66-67 0.16184266458598273 1103 68-69 0.3984869330679217 1103 70-71 0.19460771707021962 1103 72-73 0.24720493321714088 1103 74-75 0.22098686493838215 1103 76-77 0.34618916835175284 1103 78-79 -0.005912509755766848 1103 80-81 0.16357207596670875 1103 82-83 0.2848265424478349 1103 84-85 0.5828855378377682 1103 86-87 0.7214572457893844 1103 88-89 0.5794900735329094 1103 90-91 0.2534085594248445 1103 92-93 0.4250071269985014 1103 94-95 0.40541237297808763 1103 96-97 0.6579445828039994 1103 98-99 0.6974601333960813 1103 100-101 1.0036326146571888 1104 1 0.23873792890256595 1104 2 0.22595903289698072 1104 3 0.1984648199011474 1104 4 0.20500754475744287 1104 5 0.16733086622571847 1104 6 0.1259570446772642 1104 7 0.13399349868612376 1104 8 0.11790845289725382 1104 9 0.2505460275733853 1104 10-11 0.2183621584456361 1104 12-13 0.22445991996594472 1104 14-15 0.1984735947659928 1104 16-17 0.367120462895727 1104 18-19 0.3027895975986823 1104 20-21 0.26309557508664483 1104 22-23 0.23436274360377496 1104 24-25 0.16828377446353215 1104 26-27 0.1398549046466684 1104 28-29 0.29502858732939785 1104 30-31 0.3480519543276728 1104 32-33 0.27256405767569447 1104 34-35 0.268404206164476 1104 36-37 0.42613475143758706 1104 38-39 0.5051762740604744 1104 40-41 0.49085959722041395 1104 42-43 0.39676467025174134 1104 44-45 0.39311701028814383 1104 46-47 0.4974306817077476 1104 48-49 0.6143938591080627 1104 50-51 0.6388296110499851 1104 52-53 0.7276721485567492 1104 54-55 0.5707442108715597 1104 56-57 0.5162797228893012 1104 58-59 0.7323463548820897 1104 60-61 0.8440161651619391 1104 62-63 0.6207517882409093 1104 64-65 0.6449062702608614 1104 66-67 0.4592486478291349 1104 68-69 0.4845927727719257 1104 70-71 0.48909101074688976 1104 72-73 0.5292911923544423 1104 74-75 0.6001347241011459 1104 76-77 0.4555221888611065 1104 78-79 0.6102043618382922 1104 80-81 0.7247890304729658 1104 82-83 0.7685375484537786 1104 84-85 0.9056323400430415 1104 86-87 0.7819229382723343 1104 88-89 0.6022563234239051 1104 90-91 0.7235708956940172 1104 92-93 0.8030244596618914 1104 94-95 0.725652430781313 1104 96-97 0.9992929925923448 1104 98-99 0.9106908576943376 1104 100-101 1.4444441714271292 1105 1 0.03813505064507794 1105 2 0.1199936206721901 1105 3 0.06935335423359135 1105 4 0.125047128074236 1105 5 0.13160798119461248 1105 6 0.11987534322967264 1105 7 0.06870946986650495 1105 8 0.22349814112571664 1105 9 0.035020027208247484 1105 10-11 0.14615931776054225 1105 12-13 0.12612970884610064 1105 14-15 0.2464852342720505 1105 16-17 0.12310724111537752 1105 18-19 0.15329245918672285 1105 20-21 0.1620903149506958 1105 22-23 0.236266830792907 1105 24-25 0.2088202924546252 1105 26-27 0.04030954391194541 1105 28-29 0.10984891452678625 1105 30-31 0.15219093780904558 1105 32-33 0.15752074357837387 1105 34-35 0.19587756812691737 1105 36-37 0.10857454960500235 1105 38-39 0.14972549787290035 1105 40-41 0.19771847846252655 1105 42-43 0.43544340506733903 1105 44-45 0.45965389341945695 1105 46-47 0.3581144729216277 1105 48-49 0.4479719422277384 1105 50-51 0.3175194455991317 1105 52-53 0.5020016002907681 1105 54-55 0.35608608001906106 1105 56-57 0.1900514097048287 1105 58-59 0.1403072055000436 1105 60-61 0.31564536526671816 1105 62-63 0.3225635319971474 1105 64-65 0.19279057372942532 1105 66-67 0.17601096535824468 1105 68-69 0.2941109596655096 1105 70-71 0.5967590877015141 1105 72-73 0.5599818233326204 1105 74-75 0.18988943189636132 1105 76-77 0.23054190114119066 1105 78-79 0.3401940823472671 1105 80-81 0.4253800019840881 1105 82-83 0.6340792921835536 1105 84-85 0.791414560360181 1105 86-87 0.7113701741873122 1105 88-89 0.49493519582856393 1105 90-91 0.6307154727002384 1105 92-93 0.6798640519748957 1105 94-95 0.8717740065995052 1105 96-97 0.6698402663443161 1105 98-99 0.34858262272395635 1105 100-101 0.7279600087227642 1106 1 0.14154870526355268 1106 2 0.2067419746322443 1106 3 0.1553572336970248 1106 4 0.20810042971617548 1106 5 0.2517893870903052 1106 6 0.027642466143092292 1106 7 0.03538569859092888 1106 8 0.06657794332772227 1106 9 0.19617485058337536 1106 10-11 0.13659384498509297 1106 12-13 0.21161792019490377 1106 14-15 0.14598459224817617 1106 16-17 0.15105918494511172 1106 18-19 0.24853055442481775 1106 20-21 0.013376411718716952 1106 22-23 0.12142993217803877 1106 24-25 0.10335762546011296 1106 26-27 0.008846958877924749 1106 28-29 0.003054389487857634 1106 30-31 0.12017511943350456 1106 32-33 0.19758235055048345 1106 34-35 0.2816150759814633 1106 36-37 0.008371014375654795 1106 38-39 0.12850455969843466 1106 40-41 0.3262017995017885 1106 42-43 0.2770991436679964 1106 44-45 0.20725654893949752 1106 46-47 0.1410352717632577 1106 48-49 0.007365980967726671 1106 50-51 0.12125138014001635 1106 52-53 -0.12692225840933702 1106 54-55 -0.057288506763448765 1106 56-57 -0.20160364589170854 1106 58-59 -0.057422488787310044 1106 60-61 -0.10658437000088128 1106 62-63 0.04805392895914906 1106 64-65 -0.18637233641454998 1106 66-67 0.004815981340541953 1106 68-69 0.06949480798395058 1106 70-71 0.2350332728775939 1106 72-73 0.3202781105221959 1106 74-75 0.16512028729072625 1106 76-77 0.04502773563563167 1106 78-79 -0.09002680118686612 1106 80-81 0.22883873487483797 1106 82-83 0.3036282362591791 1106 84-85 0.3726613160905856 1106 86-87 0.5715592297292105 1106 88-89 0.42663819831470207 1106 90-91 0.29359829651249214 1106 92-93 0.544127227124477 1106 94-95 0.359869244694746 1106 96-97 0.7990407417151886 1106 98-99 0.6404302854838413 1106 100-101 1.0691376444356813 1107 1 0.06084577338310737 1107 2 0.10895204766213595 1107 3 0.0783877344156636 1107 4 0.1577888612138807 1107 5 0.06466811586199128 1107 6 0.005081127709857469 1107 7 -0.0010784853665626315 1107 8 0.12137589772626711 1107 9 0.031201623822859403 1107 10-11 0.09685150980211432 1107 12-13 0.08829197225865215 1107 14-15 0.14639851289134498 1107 16-17 0.17058959651885175 1107 18-19 0.10571242225660171 1107 20-21 0.12541918372740923 1107 22-23 0.1047557610455101 1107 24-25 0.04136133727233471 1107 26-27 0.04214908366012082 1107 28-29 0.1403441273579631 1107 30-31 0.15369517139762223 1107 32-33 0.15315324926588403 1107 34-35 0.029418648859227403 1107 36-37 0.02400897387534684 1107 38-39 -0.09161536761947531 1107 40-41 -0.04374236037874368 1107 42-43 0.12079882340434267 1107 44-45 0.06485829085292494 1107 46-47 -0.0515829032070485 1107 48-49 0.05628914495080295 1107 50-51 -0.03347582234238189 1107 52-53 -0.0207843573473383 1107 54-55 -0.2735907133270743 1107 56-57 -0.1194888090770192 1107 58-59 -0.13964464416390143 1107 60-61 -0.16942886650136302 1107 62-63 0.004509539965233955 1107 64-65 -0.06360005825611381 1107 66-67 -0.025626926281212548 1107 68-69 -0.030564632399098457 1107 70-71 -0.013154766789856609 1107 72-73 0.0838261781144034 1107 74-75 0.2288107987318284 1107 76-77 0.02745302859964127 1107 78-79 0.02594022679084418 1107 80-81 0.3684708374444341 1107 82-83 0.24383989039699117 1107 84-85 0.36057849937471076 1107 86-87 0.3484085322212245 1107 88-89 0.05807507949002044 1107 90-91 0.30199530813705167 1107 92-93 0.4820923982560643 1107 94-95 0.6004611668814235 1107 96-97 0.7143602862583442 1107 98-99 0.7204517053287809 1107 100-101 1.2748621575305812 1108 1 -0.01840011572272715 1108 2 0.08106772953470198 1108 3 -0.02812385143249685 1108 4 0.07999926234496257 1108 5 0.07027912608213427 1108 6 0.004532692919894998 1108 7 -0.012000498918773417 1108 8 0.004798248337358757 1108 9 0.14372836409948775 1108 10-11 -0.031016224701907902 1108 12-13 0.04923737590176103 1108 14-15 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-1.293863896166883 2316 54-55 -1.0465051810103958 2316 56-57 -0.9667166515784018 2316 58-59 -1.7231713465425358 2316 60-61 -1.1972683494419627 2316 62-63 -1.020020738325833 2316 64-65 -0.9660981976685932 2316 66-67 -0.9704061507109785 2316 68-69 -1.2129458354186724 2316 70-71 -1.1281478007879073 2316 72-73 -0.7678397197924021 2316 74-75 -0.7537164214985381 2316 76-77 -0.7133194948852655 2316 78-79 -1.0366563987778221 2316 80-81 -0.9020229799340669 2316 82-83 -1.172507245713124 2316 84-85 -0.9043854349401812 2316 86-87 -0.7827053489407625 2316 88-89 -1.015293852612917 2316 90-91 -1.031107633053292 2316 92-93 -1.2765306820845304 2316 94-95 -1.2017517767708377 2316 96-97 -1.5555263051547499 2316 98-99 -2.504183699694476 2316 100-101 -3.3540324601127836 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 5.0 4 16.0 5 46.0 6 94.0 7 228.0 8 563.0 9 1051.0 10 1666.0 11 2758.0 12 3915.0 13 5259.0 14 7007.0 15 9270.0 16 11593.0 17 14719.0 18 12683.0 19 9007.0 20 8254.0 21 8673.0 22 9385.0 23 10524.0 24 12337.0 25 14950.0 26 18152.0 27 22822.0 28 28290.0 29 35027.0 30 43694.0 31 54252.0 32 67329.0 33 85540.0 34 113198.0 35 153115.0 36 215597.0 37 280919.0 38 169853.0 39 9282.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.73567213633594 19.533604387580734 15.295455144871509 22.43526833121182 2 15.751451906633799 20.76844171275013 39.29857363359762 24.181532747018455 3 20.15211104564372 24.160727756761126 29.945291934811134 25.74186926278401 4 13.098407023640668 17.046221018183637 37.429601214647946 32.42577074352776 5 13.779389588967328 37.48765638998244 34.116246099141655 14.616707921908581 6 31.743363986354638 36.4890540124185 17.12289983318021 14.644682168046655 7 28.44874246743406 32.94302312993902 20.476296878166035 18.131937524460888 8 26.542180981433315 34.12693015496754 19.50799263883376 19.822896224765383 9 25.92777896516214 17.228376574170966 20.477962300392207 36.36588216027468 10-11 25.095796474301146 26.493259203539576 27.08531680494297 21.325627517216304 12-13 26.444545296202303 23.78074041382067 27.632942162673423 22.141772127303604 14-15 22.823954259035727 25.431284984818586 26.56006311969947 25.184697636446213 16-17 22.014834748479608 28.690332779117828 27.110436923521036 22.18439554888153 18-19 21.78090680597172 27.27586259539866 29.644296065807207 21.29893453282241 20-21 22.615427638792124 25.80960337969684 29.798705966930267 21.77626301458077 22-23 22.114679012084384 25.96021232767633 29.876664946885118 22.048443713354168 24-25 22.49270683850123 26.125027410074136 29.830106115152844 21.552159636271785 26-27 22.083084239295435 26.21155118591893 29.94992212305494 21.755442451730694 28-29 21.609418501689063 26.9494655234151 29.803349812218432 21.63776616267741 30-31 21.976106410854264 26.223130815179978 29.70300945972953 22.097753314236225 32-33 21.853988901299427 26.277958846742788 29.686612474362057 22.181439777595727 34-35 22.673579221255064 26.180606564868175 29.417344789335825 21.728469424540933 36-37 22.47156275762143 25.94200967615685 29.707446144109785 21.878981422111934 38-39 22.097160221308854 26.48923051941518 29.631875913821947 21.781733345454015 40-41 22.29363120397093 26.370662693701153 29.469117803192486 21.866588299135437 42-43 22.07555045573922 26.59297069660492 28.970017914512074 22.36146093314379 44-45 22.305186130994308 26.339001634219372 28.883144640558477 22.47266759422784 46-47 22.70205003565053 26.81683260210711 28.445272531395887 22.03584483084647 48-49 22.75424294836987 26.928752469957168 28.189658013937585 22.127346567735376 50-51 22.283145169208925 27.06097225436454 28.414824087737372 22.241058488689163 52-53 22.8365520565051 27.229665940009305 27.751082786547666 22.18269921693793 54-55 22.261252133845918 26.878617129057776 28.206736707701275 22.65339402939503 56-57 22.777516748480267 26.95129424649016 27.8959619731594 22.375227031870175 58-59 23.12984805845139 26.724026935171587 27.78953695673374 22.356588049643285 60-61 22.60806978432546 26.891091088034138 28.586385726640117 21.914453401000287 62-63 22.407948998047033 27.011748327070983 28.35675410823618 22.2235485666458 64-65 22.188044070844555 27.251249551903385 28.140745195519344 22.41996118173272 66-67 22.342258944144874 27.127967603963942 27.7690668652269 22.760706586664288 68-69 22.460437246064544 26.814127051867892 28.02281645866078 22.702619243406787 70-71 22.70140974766381 26.951227678532707 27.817734487519203 22.529628086284287 72-73 22.6051228387677 26.668371411362063 28.23820534100908 22.488300408861157 74-75 22.356129211338953 27.498247805419656 27.558238784219842 22.587384199021546 76-77 22.446415547627655 27.05852635903301 27.643658100417547 22.851399992921795 78-79 22.47046013210876 27.037532600382807 27.767094120896584 22.72491314661185 80-81 22.75085106309145 27.24134101863837 27.464300462575782 22.5435074556944 82-83 22.62122684871096 27.124838156565545 27.798759249497596 22.4551757452259 84-85 22.490039287548765 27.344932183860177 27.583225743756334 22.58180278483473 86-87 22.518967154135712 27.29073670008994 27.3304684810731 22.859827664701246 88-89 22.364882407742943 27.251392932663006 27.11314098158538 23.27058367800867 90-91 22.396299959074337 26.999621290092136 27.488889498280532 23.115189252552995 92-93 22.7562036864469 26.91715680813352 27.56061390110266 22.766025604316926 94-95 22.331282168750803 26.91179992295118 27.664275376654206 23.09264253164381 96-97 22.056618183080502 26.84378796820217 28.069011004269797 23.03058284444753 98-99 22.081211842233618 26.937842610615213 27.378925300948854 23.60202024620232 100-101 21.73856289021708 26.859259392983315 27.584806252360742 23.817371464438867 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 163.0 1 136.0 2 139.5 3 170.5 4 150.5 5 139.5 6 224.0 7 462.0 8 774.0 9 988.5 10 994.0 11 927.5 12 984.5 13 1118.5 14 1270.0 15 1435.5 16 1600.0 17 1773.0 18 1949.0 19 2130.5 20 2395.5 21 2751.0 22 3193.0 23 3707.5 24 4551.0 25 5686.0 26 7127.0 27 9045.0 28 11042.0 29 13488.5 30 16567.5 31 20000.0 32 23922.5 33 26551.0 34 28609.0 35 31260.5 36 34132.5 37 37051.5 38 39550.5 39 41034.5 40 42330.5 41 45110.0 42 47551.5 43 49599.0 44 51407.5 45 53158.0 46 54353.0 47 58517.0 48 68125.0 49 67012.0 50 56627.5 51 51338.5 52 48913.0 53 47461.0 54 45578.5 55 42009.0 56 37875.0 57 33905.0 58 30729.5 59 26751.0 60 21876.0 61 17969.0 62 14891.0 63 11531.0 64 8409.0 65 6589.0 66 5177.0 67 4167.0 68 3090.0 69 2131.0 70 1624.5 71 1277.5 72 1045.0 73 908.0 74 776.5 75 662.5 76 507.5 77 311.0 78 194.0 79 136.0 80 112.5 81 86.5 82 56.5 83 35.0 84 20.0 85 10.0 86 5.5 87 3.0 88 1.0 89 1.0 90 1.5 91 2.5 92 1.5 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019568711157496206 2 0.014711229664500692 3 0.010755851877347204 4 0.0 5 0.0032614518595827004 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0011796740768703386 14-15 3.1226666740685435E-4 16-17 0.0 18-19 0.0012490666696274174 20-21 0.0 22-23 1.040888891356181E-4 24-25 0.0 26-27 0.0014225481515201142 28-29 0.002775703710283149 30-31 0.0013878518551415746 32-33 0.006696385201058099 34-35 0.0018389037080625864 36-37 5.551407420566299E-4 38-39 9.021037058420237E-4 40-41 2.081777782712362E-4 42-43 0.004545214825588658 44-45 0.0014572444478986536 46-47 2.4287407464977558E-4 48-49 5.898370384351693E-4 50-51 1.7348148189269682E-4 52-53 1.7348148189269682E-4 54-55 0.0011102814841132598 56-57 0.01380912595865867 58-59 0.02480785191065565 60-61 0.02040142227058115 62-63 0.01318459262384496 64-65 0.018007377820461933 66-67 0.002463437042876295 68-69 7.633185203278662E-4 70-71 1.7348148189269682E-4 72-73 0.0 74-75 0.0018042074116840475 76-77 0.0022899555609835983 78-79 0.00985374817150518 80-81 0.016029688926885187 82-83 0.017660414856676538 84-85 0.02886731858694476 86-87 0.011380385212160914 88-89 0.040941629726676455 90-91 0.04576441492329343 92-93 0.02917958525435161 94-95 0.029908207478300936 96-97 0.050379022341639164 98-99 0.05249549642073007 100-101 0.05912248902903109 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1441076.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.0997120998967 #Duplication Level Percentage of deduplicated Percentage of total 1 81.19269098467167 50.42042734763989 2 11.56121808325698 14.35896628988756 3 3.2467922421685733 6.0487459046053935 4 1.3138989487909214 3.263709857930926 5 0.7317871580639534 2.2721885917086557 6 0.39176518839236657 1.459710324595665 7 0.2818252015381146 1.2250884714608599 8 0.19900114048385947 0.9886330825279017 9 0.156981920504621 0.8773678861403252 >10 0.8299404765392322 9.59377176060266 >50 0.05946628070996021 2.5352116935397193 >100 0.031169211152544875 3.672398260200223 >500 0.0016757243841000605 0.7089397310070661 >1k 0.0016757243841000605 2.1576215455573697 >5k 1.1171495894000404E-4 0.4172192525958015 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6014 0.4173270528410715 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4885 0.33898281561832966 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4484 0.31115638592274103 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2945 0.20436118566959688 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2590 0.17972681524083392 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2208 0.15321884480762987 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2113 0.14662654849570736 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1718 0.11921647435666127 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1583 0.10984847433445566 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1574 0.10922394099964193 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 6.939259275707873E-5 0.0 24-25 0.0 0.0 0.0 1.040888891356181E-4 0.0 26-27 0.0 0.0 0.0 1.7348148189269682E-4 0.0 28-29 0.0 0.0 0.0 5.551407420566299E-4 0.0 30-31 0.0 6.939259275707873E-5 0.0 0.0012837629660059566 3.469629637853937E-5 32-33 0.0 6.939259275707873E-5 0.0 0.0024981333392548348 6.939259275707873E-5 34-35 0.0 6.939259275707873E-5 0.0 0.0037818963052607913 6.939259275707873E-5 36-37 0.0 6.939259275707873E-5 0.0 0.005482014827809221 6.939259275707873E-5 38-39 0.0 6.939259275707873E-5 0.0 0.007876059277928437 6.939259275707873E-5 40-41 0.0 6.939259275707873E-5 0.0 0.010825244470104283 6.939259275707873E-5 42-43 0.0 6.939259275707873E-5 0.0 0.016307259297913504 6.939259275707873E-5 44-45 0.0 6.939259275707873E-5 0.0 0.024877244503412728 6.939259275707873E-5 46-47 0.0 6.939259275707873E-5 0.0 0.033932977858211505 6.939259275707873E-5 48-49 0.0 6.939259275707873E-5 0.0 0.0548201482780922 6.939259275707873E-5 50-51 0.0 6.939259275707873E-5 0.0 0.0747358223993738 1.040888891356181E-4 52-53 0.0 6.939259275707873E-5 0.0 0.0933330372582709 1.3878518551415747E-4 54-55 0.0 6.939259275707873E-5 0.0 0.11959813361682521 1.3878518551415747E-4 56-57 0.0 6.939259275707873E-5 0.0 0.152455526287302 1.3878518551415747E-4 58-59 0.0 6.939259275707873E-5 0.0 0.19301549675381452 1.3878518551415747E-4 60-61 0.0 6.939259275707873E-5 0.0 0.24134743760911986 1.3878518551415747E-4 62-63 0.0 6.939259275707873E-5 0.0 0.3113992599973908 1.3878518551415747E-4 64-65 0.0 6.939259275707873E-5 0.0 0.37374850458962605 1.3878518551415747E-4 66-67 0.0 6.939259275707873E-5 0.0 0.45903200108807585 1.3878518551415747E-4 68-69 0.0 6.939259275707873E-5 0.0 0.5735644754336343 1.3878518551415747E-4 70-71 0.0 6.939259275707873E-5 0.0 0.642506016337792 2.081777782712362E-4 72-73 0.0 6.939259275707873E-5 0.0 0.739829127679595 2.081777782712362E-4 74-75 0.0 6.939259275707873E-5 0.0 0.8626887131560028 2.081777782712362E-4 76-77 0.0 6.939259275707873E-5 0.0 0.9581729207897431 2.081777782712362E-4 78-79 0.0 6.939259275707873E-5 0.0 1.047828150631889 2.081777782712362E-4 80-81 0.0 6.939259275707873E-5 0.0 1.1924076176412626 2.081777782712362E-4 82-83 0.0 6.939259275707873E-5 0.0 1.3732794106625883 2.081777782712362E-4 84-85 0.0 6.939259275707873E-5 0.0 1.5852043889427068 2.081777782712362E-4 86-87 0.0 6.939259275707873E-5 0.0 1.8836966266872808 2.081777782712362E-4 88-89 0.0 6.939259275707873E-5 0.0 2.2224712645273392 2.081777782712362E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 6160 0.0 23.144667 1 TACATGG 6130 0.0 22.706577 2 ACCGTCG 105 0.002198981 22.614637 8 GAGTACT 5925 0.0 22.442879 12-13 ACATGGG 5945 0.0 22.209152 3 GTACTTT 6150 0.0 21.27283 14-15 AGTACTT 6115 0.0 20.464119 12-13 GTAAGGT 685 0.0 20.104868 4 AGAGTAC 9460 0.0 19.87984 10-11 GTACACG 240 1.406388E-7 19.80155 1 TAAGGTG 900 0.0 19.52329 5 ATACCGT 150 7.7361055E-4 18.994976 6 ACTTTTT 7005 0.0 18.879732 16-17 TACTTTT 6735 0.0 18.578976 14-15 GTATAGG 500 0.0 18.059011 1 CATGGGG 2755 0.0 17.926914 4 GTATCAA 14265 0.0 17.390383 1 GGTAAGG 740 0.0 17.328903 3 GTCTTAC 495 0.0 17.281351 1 GGTATCA 11855 0.0 17.077269 1 >>END_MODULE