##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936507_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2330447 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.468279690548638 31.0 31.0 34.0 30.0 34.0 2 31.53467682380247 33.0 31.0 34.0 30.0 34.0 3 31.56360775422054 33.0 31.0 34.0 28.0 34.0 4 35.1653056259164 37.0 35.0 37.0 32.0 37.0 5 35.18510569002427 37.0 35.0 37.0 33.0 37.0 6 35.19272997841187 37.0 35.0 37.0 32.0 37.0 7 35.09811465354071 37.0 35.0 37.0 32.0 37.0 8 35.053396193949055 37.0 35.0 37.0 32.0 37.0 9 36.55690560652098 39.0 37.0 39.0 32.0 39.0 10-11 36.41471443032174 39.0 37.0 39.0 32.0 39.0 12-13 36.33971208098704 39.0 37.0 39.0 32.0 39.0 14-15 37.43217309812238 40.0 37.0 41.0 32.0 41.0 16-17 37.35796201329616 40.0 37.0 41.0 32.0 41.0 18-19 37.32516851917251 40.0 37.0 41.0 32.0 41.0 20-21 37.1646963007526 39.5 36.5 41.0 31.0 41.0 22-23 37.28893212332227 40.0 37.0 41.0 31.0 41.0 24-25 37.32637279457546 40.0 37.0 41.0 31.0 41.0 26-27 37.23495642681426 39.5 37.0 41.0 31.0 41.0 28-29 37.12862403650459 39.0 36.0 41.0 31.0 41.0 30-31 36.95740366547705 39.0 36.0 41.0 30.0 41.0 32-33 36.791458033587546 39.0 36.0 41.0 30.0 41.0 34-35 36.59859739354725 39.0 35.5 41.0 30.0 41.0 36-37 36.39446874355006 39.0 35.0 40.5 30.0 41.0 38-39 36.19984642431258 39.0 35.0 40.0 30.0 41.0 40-41 36.06121808391266 39.0 35.0 40.0 29.0 41.0 42-43 35.82369004744584 38.0 35.0 40.0 28.5 41.0 44-45 35.562064488057445 38.0 35.0 40.0 27.5 41.0 46-47 35.41486075418149 38.0 35.0 40.0 27.0 41.0 48-49 35.26724744222889 38.0 34.0 40.0 26.0 41.0 50-51 34.28342073430548 37.5 33.0 39.5 25.0 40.5 52-53 34.41277360094436 38.0 33.5 39.5 25.5 40.5 54-55 34.87014079273204 38.0 34.0 40.0 26.0 41.0 56-57 34.704586287523384 38.0 34.0 40.0 25.5 41.0 58-59 34.458563743350524 38.0 34.0 40.0 25.0 41.0 60-61 34.085596239691355 37.5 33.0 40.0 23.0 41.0 62-63 33.76129579432616 37.0 33.0 40.0 23.0 41.0 64-65 33.41308319820189 36.5 32.5 39.5 22.0 41.0 66-67 32.89126528086672 36.0 32.0 39.0 21.5 41.0 68-69 32.47588338202928 35.0 31.5 39.0 20.5 40.0 70-71 32.02616515200732 35.0 31.0 38.0 19.0 40.0 72-73 31.613300795941722 35.0 31.0 37.0 18.0 39.5 74-75 31.205185099682595 35.0 31.0 37.0 17.5 39.0 76-77 30.761839681400176 34.5 30.0 36.0 12.5 39.0 78-79 30.24915627774414 34.0 30.0 36.0 7.5 38.0 80-81 29.728186051860437 34.0 29.0 35.0 7.0 37.0 82-83 29.2963105361332 34.0 29.0 35.0 2.0 37.0 84-85 28.93042793936099 34.0 29.0 35.0 2.0 36.0 86-87 28.6467896502259 34.0 29.0 35.0 2.0 36.0 88-89 28.2239338633318 34.0 27.5 35.0 2.0 35.5 90-91 27.7643814684479 33.5 27.0 35.0 2.0 35.0 92-93 27.29958050966188 33.0 26.0 35.0 2.0 35.0 94-95 26.789243651539813 33.0 25.0 35.0 2.0 35.0 96-97 26.18004185463132 33.0 24.5 35.0 2.0 35.0 98-99 25.445234969943535 32.5 21.0 35.0 2.0 35.0 100-101 22.512261810717 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.635942352341587 1101 2 0.5953203713991719 1101 3 0.5792139577544049 1101 4 0.6254356776283387 1101 5 0.5830533210201878 1101 6 0.6649298752209702 1101 7 0.6698650128019423 1101 8 0.7324396191137836 1101 9 0.8971865198094733 1101 10-11 0.9416219453458012 1101 12-13 0.9411552972895549 1101 14-15 1.0723111611531664 1101 16-17 1.2116663690340133 1101 18-19 1.1300276743341655 1101 20-21 1.1780896869627497 1101 22-23 1.0330488978164851 1101 24-25 1.0521320058021004 1101 26-27 1.1743956862205707 1101 28-29 1.0322241555816163 1101 30-31 1.1580729863748118 1101 32-33 1.1075633887365086 1101 34-35 1.0843959022717158 1101 36-37 1.035465927480665 1101 38-39 0.9916915561608803 1101 40-41 1.015768854775331 1101 42-43 1.1079938125841906 1101 44-45 1.1627341216674125 1101 46-47 1.1391387503262607 1101 48-49 1.2742810649364316 1101 50-51 1.0628260514824674 1101 52-53 1.2101067327034016 1101 54-55 1.261890240318003 1101 56-57 1.080363027549808 1101 58-59 1.1927430517190984 1101 60-61 1.227035955040158 1101 62-63 1.2429230754840646 1101 64-65 1.1831972691108845 1101 66-67 1.349989068671718 1101 68-69 1.2646897022061125 1101 70-71 1.192475905768454 1101 72-73 1.0564900918000326 1101 74-75 0.9797741519754695 1101 76-77 1.201890450675915 1101 78-79 1.0690633389917075 1101 80-81 0.9781957015130303 1101 82-83 0.971509614931815 1101 84-85 0.9280446621614473 1101 86-87 1.0894600437443387 1101 88-89 1.1358204287626492 1101 90-91 1.1777811370027038 1101 92-93 0.97332011621112 1101 94-95 1.0906433580605324 1101 96-97 0.9419056288183931 1101 98-99 0.8275767051009026 1101 100-101 1.070636764015088 1102 1 0.5216867053154708 1102 2 0.5958991025729254 1102 3 0.619182645664246 1102 4 0.565796699077211 1102 5 0.4672574989040541 1102 6 0.5083289492510943 1102 7 0.4811501223518775 1102 8 0.5628143809249053 1102 9 0.7615125215590126 1102 10-11 0.8046770059695305 1102 12-13 0.8102139256606478 1102 14-15 0.8363568883328085 1102 16-17 0.7105262834555433 1102 18-19 0.7414527092572314 1102 20-21 0.688711978729728 1102 22-23 0.5490735635044075 1102 24-25 0.628505660585887 1102 26-27 0.40823073490943074 1102 28-29 0.38678165827714395 1102 30-31 0.7441083366565735 1102 32-33 0.6610886053951504 1102 34-35 0.5591395101636536 1102 36-37 0.33525164225075343 1102 38-39 0.19532302540483926 1102 40-41 0.25645321127543497 1102 42-43 0.42851001281881196 1102 44-45 0.6073747022718834 1102 46-47 0.5451058480498219 1102 48-49 0.6194355105073015 1102 50-51 0.6822397381038812 1102 52-53 0.7975273505365408 1102 54-55 0.8668103241333682 1102 56-57 0.7802811660146816 1102 58-59 0.84262828027871 1102 60-61 0.7009497162545273 1102 62-63 0.8647393123370577 1102 64-65 0.8245207304615647 1102 66-67 0.9665160925143397 1102 68-69 0.9551350645583696 1102 70-71 0.9208390734602077 1102 72-73 0.6214706255804678 1102 74-75 0.6751295111405717 1102 76-77 0.7108174479024107 1102 78-79 0.6101876879986072 1102 80-81 0.8327392180285855 1102 82-83 0.8692654475926815 1102 84-85 0.5513178987160643 1102 86-87 0.6904610304057606 1102 88-89 0.62556262532188 1102 90-91 0.5985408451068892 1102 92-93 0.7492601358426292 1102 94-95 0.5707747179580025 1102 96-97 0.4752924416307529 1102 98-99 0.9301501834045247 1102 100-101 1.0278549467271567 1103 1 0.3852391502181689 1103 2 0.3945308319087104 1103 3 0.3941157137575715 1103 4 0.6088148151209083 1103 5 0.6369416487638588 1103 6 0.5529248134732043 1103 7 0.5175473877210379 1103 8 0.5825754380480248 1103 9 0.42673776162928334 1103 10-11 0.6734975337310729 1103 12-13 0.3669756452867574 1103 14-15 0.693038625997147 1103 16-17 0.7379222447147384 1103 18-19 0.8036931634130795 1103 20-21 0.8767992436471417 1103 22-23 0.5686712337387192 1103 24-25 0.7252858244225351 1103 26-27 0.5831165979235564 1103 28-29 0.6700965135682964 1103 30-31 0.597957172539914 1103 32-33 0.7534967271364934 1103 34-35 0.8224143963649837 1103 36-37 0.778495196379275 1103 38-39 0.8110720886168608 1103 40-41 0.7906820679473228 1103 42-43 0.8103276591202331 1103 44-45 0.8250005654014387 1103 46-47 1.0119412947656983 1103 48-49 0.8889094465173528 1103 50-51 0.8311541506384614 1103 52-53 0.9100888768447177 1103 54-55 0.9588505766128748 1103 56-57 0.9587567643519961 1103 58-59 1.1226001721615617 1103 60-61 1.0881274873588467 1103 62-63 1.1538423620996099 1103 64-65 0.9759165938080372 1103 66-67 1.2265992626100441 1103 68-69 0.9381699929338154 1103 70-71 0.9591817572600121 1103 72-73 0.7638432937600079 1103 74-75 0.8262736273319291 1103 76-77 1.0216537400111037 1103 78-79 0.938157115346467 1103 80-81 0.9967553752207081 1103 82-83 0.7444424417634892 1103 84-85 0.7674494981980828 1103 86-87 0.8044752273532332 1103 88-89 0.74984581758725 1103 90-91 0.8264106283436341 1103 92-93 0.8030979067917237 1103 94-95 0.7571210468178009 1103 96-97 0.8665456310084956 1103 98-99 0.8957712734876146 1103 100-101 0.8214868581567352 1104 1 0.3561780756898152 1104 2 0.4247749911878813 1104 3 0.4144343476885446 1104 4 0.4272351621578494 1104 5 0.38885889906758564 1104 6 0.41450689135771057 1104 7 0.5473262090632716 1104 8 0.5469363898521493 1104 9 0.6891195022700884 1104 10-11 0.6644430903150962 1104 12-13 0.7129143602617489 1104 14-15 0.5709429886666015 1104 16-17 0.554200016473871 1104 18-19 0.5201448147640093 1104 20-21 0.43617997558797583 1104 22-23 0.44559374645879757 1104 24-25 0.46649899241963766 1104 26-27 0.39817676348057773 1104 28-29 0.42991047568975205 1104 30-31 0.4133145593727008 1104 32-33 0.48905707764990325 1104 34-35 0.4960276397292418 1104 36-37 0.31407820568809797 1104 38-39 0.3253733248590365 1104 40-41 0.25233378748045254 1104 42-43 0.49049913624354957 1104 44-45 0.4206897248985868 1104 46-47 0.23143410822280686 1104 48-49 0.6020532131126757 1104 50-51 0.4842527642981409 1104 52-53 0.5105430257150587 1104 54-55 0.37035740896006075 1104 56-57 0.516324605552569 1104 58-59 0.4563414947878215 1104 60-61 0.4137614144050161 1104 62-63 0.5225992886089657 1104 64-65 0.41524257042363644 1104 66-67 0.5159570382535392 1104 68-69 0.3913340195982187 1104 70-71 0.33420259642878136 1104 72-73 0.2404897658454388 1104 74-75 0.3865915839314553 1104 76-77 0.36472549055553927 1104 78-79 0.16745729536320653 1104 80-81 0.4085711231022202 1104 82-83 0.37765810278941103 1104 84-85 0.2504770126367504 1104 86-87 0.31113187999239855 1104 88-89 0.25708549918159207 1104 90-91 0.2074516718232644 1104 92-93 0.09054970539675722 1104 94-95 0.2580670655589259 1104 96-97 0.2743690525689253 1104 98-99 0.2024843579409321 1104 100-101 0.020841222818891225 1105 1 0.24241801856492273 1105 2 0.2339173001218917 1105 3 0.3191313263569384 1105 4 0.33606107785492867 1105 5 0.17526244864937013 1105 6 0.32040713003600274 1105 7 0.43636512363391944 1105 8 0.48209480851332387 1105 9 0.46817132351372237 1105 10-11 0.5326203875407671 1105 12-13 0.2904869188845538 1105 14-15 0.3578990016536352 1105 16-17 0.3487378187162946 1105 18-19 0.28206375014323726 1105 20-21 0.37925280700609676 1105 22-23 -0.005319747816727727 1105 24-25 0.2791820895418198 1105 26-27 0.08503971237691843 1105 28-29 -0.05164332631582624 1105 30-31 0.01731945206615393 1105 32-33 -0.16655565096597513 1105 34-35 0.10107842406866752 1105 36-37 0.2624898425587432 1105 38-39 0.07148211450775932 1105 40-41 -0.00852797789300297 1105 42-43 0.05724200232852894 1105 44-45 0.11959893128270238 1105 46-47 0.3378019935162513 1105 48-49 0.2675811178894776 1105 50-51 0.2612422317198977 1105 52-53 0.36679698195220567 1105 54-55 0.23238738331588138 1105 56-57 0.033181700275285664 1105 58-59 0.16835078040241314 1105 60-61 0.2703858823836356 1105 62-63 0.07077330453682151 1105 64-65 0.1853962063553638 1105 66-67 0.2202902545738894 1105 68-69 0.3088762570556227 1105 70-71 0.28418782882643256 1105 72-73 0.16393322883761385 1105 74-75 0.22493294957164522 1105 76-77 0.3172357538629491 1105 78-79 0.29292931234277475 1105 80-81 0.37189090125688296 1105 82-83 0.21704016145661598 1105 84-85 0.05241738394854778 1105 86-87 -0.023881047827504887 1105 88-89 0.18735422020149528 1105 90-91 0.0627783354165743 1105 92-93 -0.004327998413774736 1105 94-95 -0.003275968573621668 1105 96-97 0.23156115687119794 1105 98-99 0.3241894027512622 1105 100-101 0.40033265338014346 1106 1 0.4736270242684206 1106 2 0.3509452463135041 1106 3 0.513934781712635 1106 4 0.3787157649002282 1106 5 0.2673443051487112 1106 6 0.46227991646133404 1106 7 0.5218964419018732 1106 8 0.5192655969046385 1106 9 0.6229430227478474 1106 10-11 0.6658403684173564 1106 12-13 0.6805849900488425 1106 14-15 0.5858001339042076 1106 16-17 0.6391280549158509 1106 18-19 0.4136496586325009 1106 20-21 0.24183751123027974 1106 22-23 0.4767045339491176 1106 24-25 0.37170825347475045 1106 26-27 0.368120877563733 1106 28-29 0.4671229423264833 1106 30-31 0.5537068143903596 1106 32-33 0.3878584734005486 1106 34-35 0.40901566080331264 1106 36-37 0.3127073109736074 1106 38-39 0.35572940859921687 1106 40-41 0.32939879528004923 1106 42-43 0.5087337890529184 1106 44-45 0.42838916117932513 1106 46-47 0.5723611996400351 1106 48-49 0.6116572869854622 1106 50-51 0.2499626397216943 1106 52-53 0.24433794104608353 1106 54-55 0.49280140978557796 1106 56-57 0.44476192270224146 1106 58-59 0.5851913648062421 1106 60-61 0.7442247153729937 1106 62-63 0.7395957968023055 1106 64-65 0.6986971834461642 1106 66-67 0.6441811706870411 1106 68-69 0.5620511148705489 1106 70-71 0.41824965536666525 1106 72-73 0.32844859595742193 1106 74-75 0.5119801096919687 1106 76-77 0.5660379425946225 1106 78-79 0.3835600662958605 1106 80-81 0.6969809529276318 1106 82-83 0.662521015921584 1106 84-85 0.7877496229830996 1106 86-87 0.7044725219870109 1106 88-89 0.6624602639224868 1106 90-91 0.4237851238239827 1106 92-93 0.333689547245708 1106 94-95 0.43037697108036 1106 96-97 0.3933163866404179 1106 98-99 0.6199073049965911 1106 100-101 0.5182218904956493 1107 1 0.27091936631969205 1107 2 0.24923254644224002 1107 3 0.30785198045328954 1107 4 0.35539487854956064 1107 5 0.3410321940094647 1107 6 0.36070051379722656 1107 7 0.3058053897549442 1107 8 0.41498607959204037 1107 9 0.4034033284674905 1107 10-11 0.4473300606104118 1107 12-13 0.354388430019398 1107 14-15 0.13692678543161918 1107 16-17 0.19393101762761233 1107 18-19 0.3015304280684674 1107 20-21 0.30094982597415765 1107 22-23 0.11957053028287135 1107 24-25 0.22208407261993557 1107 26-27 0.2504372542136153 1107 28-29 0.2790258349081185 1107 30-31 0.3332899696851541 1107 32-33 0.024430085350616082 1107 34-35 0.15054778244837763 1107 36-37 0.2010037678306844 1107 38-39 0.13469878425089377 1107 40-41 0.27811144566855006 1107 42-43 0.3090486090106239 1107 44-45 0.24136503986500202 1107 46-47 0.46815856596445116 1107 48-49 0.42472415270070485 1107 50-51 0.32374820464959697 1107 52-53 0.4513617206553491 1107 54-55 0.5670063693120682 1107 56-57 0.30542669054186433 1107 58-59 0.3649687439639422 1107 60-61 0.4015262995788689 1107 62-63 0.5157736678218683 1107 64-65 0.3747319412973624 1107 66-67 0.5127199834650682 1107 68-69 0.5668406269753703 1107 70-71 0.42672925968511066 1107 72-73 0.35114030981941013 1107 74-75 0.5000869202443994 1107 76-77 0.42676234763696286 1107 78-79 0.4022821184087846 1107 80-81 0.32414624678349924 1107 82-83 0.4811176203726788 1107 84-85 0.2687601705477185 1107 86-87 0.3213528160983543 1107 88-89 0.25616957059809664 1107 90-91 0.19469678807104174 1107 92-93 0.14957419019858165 1107 94-95 0.44116187564713627 1107 96-97 0.4240493713808675 1107 98-99 0.3957278363447223 1107 100-101 0.19838217273112946 1108 1 0.28458348063084316 1108 2 0.25549266554373773 1108 3 0.1846719574809832 1108 4 0.356615687079298 1108 5 0.3367612598208112 1108 6 0.3190827592476708 1108 7 0.39659259799426394 1108 8 0.3061072724837359 1108 9 0.4262552591474318 1108 10-11 0.4606218346388786 1108 12-13 0.28577497815642516 1108 14-15 0.6038839944181476 1108 16-17 0.6007164888055243 1108 18-19 0.5812281199852336 1108 20-21 0.5300102662645685 1108 22-23 0.4607298813397307 1108 24-25 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2316 88-89 -3.5340105633356806 2316 90-91 -3.5770065056663043 2316 92-93 -4.139347245509686 2316 94-95 -3.887819109015851 2316 96-97 -4.13309162754684 2316 98-99 -5.219106240465383 2316 100-101 -6.012028951524858 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4382.0 3 1776.0 4 2028.0 5 2037.0 6 2328.0 7 2776.0 8 3499.0 9 4350.0 10 5711.0 11 7198.0 12 8680.0 13 10315.0 14 12134.0 15 13869.0 16 16339.0 17 19813.0 18 17191.0 19 14563.0 20 15330.0 21 17177.0 22 18772.0 23 21607.0 24 24784.0 25 28347.0 26 33081.0 27 39232.0 28 46630.0 29 55635.0 30 67472.0 31 82910.0 32 103967.0 33 132049.0 34 173226.0 35 234349.0 36 321854.0 37 413917.0 38 308561.0 39 42555.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.330411089648706 18.22748783030273 14.10706415360576 22.3350369264428 2 15.330756699505443 20.75898259870945 38.80563323222492 25.104627469560185 3 21.50175057315284 23.713592670510288 29.133116518046055 25.651540238290814 4 12.59225179353452 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23.449687912208628 26.72999964813421 27.119583734188566 22.700728705468595 50-51 21.718626058660856 27.192283257340115 28.506199574615355 22.582891109383674 52-53 23.086687184275707 27.849521703979992 26.303127075863088 22.760664035881213 54-55 22.259224889365697 26.547662598900363 27.20761700415717 23.985495507576772 56-57 23.428312737953608 26.33579605371141 26.803673590676347 23.432217617658637 58-59 24.61612074033704 26.196889657510436 26.42527324925224 22.761716352900283 60-61 23.068353976278082 26.759023702822272 28.313193168571686 21.859429152327962 62-63 22.2951535082826 27.13628140410259 27.851214455027147 22.717350632587664 64-65 22.01029126251087 28.33055880186584 26.876874525166777 22.782275410456513 66-67 22.76686188029544 26.995467618936647 26.151845173296355 24.08582532747156 68-69 22.308379344814256 26.853690466710212 27.087162555344175 23.750767633131357 70-71 23.687562873457065 26.65851753673012 26.547615520786504 23.106304069026308 72-73 23.112874633834302 25.940636254411636 28.20770379799477 22.73878531375929 74-75 22.09377668719978 28.51724818068282 26.708909036394502 22.6800660957229 76-77 22.66690188332138 26.696575236922833 26.67642300710758 23.960099872648208 78-79 22.92207793601816 26.911384312455095 27.02857794256589 23.137959808960858 80-81 23.78247830413423 26.750754475423232 26.55714837279396 22.909618847648574 82-83 23.14964879207744 26.442515542576633 27.783399527830237 22.624436137515687 84-85 22.694316195618235 27.332625893375422 27.2186128955074 22.754445015498945 86-87 22.710236591878633 27.653023757473633 26.772507709960834 22.864231940686896 88-89 22.331789928726984 27.598701067936414 25.82288444981231 24.246624553524292 90-91 22.953678296317413 26.408788691569846 26.49505376381014 24.142479248302603 92-93 23.886919713142266 27.002664610978815 26.846856978035845 22.263558697843074 94-95 23.066484384605992 26.61476162815275 27.11862661010515 23.200127377136106 96-97 21.632633091030353 26.864300174720807 28.400092188722503 23.102974545526337 98-99 22.4584399114232 27.747095881212974 26.252212058843988 23.54225214851984 100-101 22.097410361431248 26.535803148221742 26.922841579611468 24.443944910735546 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 124.0 1 100.0 2 94.5 3 133.5 4 155.5 5 163.0 6 228.5 7 432.0 8 723.5 9 937.5 10 953.5 11 905.5 12 996.5 13 1106.0 14 1237.5 15 1360.5 16 1483.5 17 1666.5 18 1833.0 19 2054.0 20 2292.5 21 2675.0 22 3209.0 23 3931.5 24 4891.0 25 6326.5 26 8513.5 27 11364.5 28 14143.5 29 17528.5 30 22121.0 31 27261.0 32 33513.0 33 38360.5 34 41896.0 35 45877.0 36 51761.0 37 57218.0 38 59410.0 39 61082.5 40 63176.0 41 66243.5 42 68394.0 43 71253.5 44 74842.5 45 78825.0 46 83684.0 47 106087.0 48 150744.5 49 147067.5 50 103521.0 51 83199.0 52 76575.0 53 74322.0 54 72897.5 55 68581.5 56 63245.5 57 57928.0 58 53565.0 59 48060.0 60 40087.0 61 33050.0 62 27642.5 63 21911.5 64 15909.5 65 12068.0 66 9303.0 67 7103.5 68 5213.0 69 3745.5 70 2875.0 71 2168.5 72 1684.5 73 1431.0 74 1161.5 75 907.5 76 662.5 77 426.0 78 262.0 79 186.0 80 137.0 81 82.0 82 55.5 83 42.0 84 25.5 85 21.5 86 18.5 87 12.5 88 9.0 89 6.5 90 6.0 91 5.5 92 5.0 93 3.0 94 1.5 95 1.0 96 0.5 97 1.5 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011757401047953461 2 0.011800311270756212 3 0.015147308649370701 4 0.017636101571930192 5 0.029050220837461654 6 0.046643412186589094 7 0.006393623197609728 8 0.026346876800888413 9 0.03488601113863563 10-11 0.038340284074257 12-13 0.029608053733897403 14-15 0.02808474082439978 16-17 0.02881421461204653 18-19 0.023300250981893172 20-21 0.05580474475497619 22-23 0.013752726408281329 24-25 0.0028749849277842406 26-27 0.0013731271296879954 28-29 2.1455111401374931E-4 30-31 0.0028320747049814907 32-33 0.0020596906945319934 34-35 0.0 36-37 2.574613368164992E-4 38-39 2.1455111401374928E-5 40-41 0.001201486238476996 42-43 0.004333932503077736 44-45 0.001780774246314119 46-47 0.0011585760156742461 48-49 5.578328964357482E-4 50-51 0.003668824049635113 52-53 0.003904830275050237 54-55 0.0057714249669698564 56-57 0.0012658515726811208 58-59 0.006736904980031729 60-61 0.003025170707593865 62-63 8.152942332522473E-4 64-65 0.0010083902358646217 66-67 0.018687402030597565 68-69 0.04490554816307773 70-71 0.05852954390295081 72-73 0.01667062155886832 74-75 0.034135082239587514 76-77 0.029178951505869903 78-79 0.05988121592123743 80-81 0.08616372738792172 82-83 0.051492267363299836 84-85 0.01928814514983606 86-87 0.021734027849592802 88-89 0.022871148753865676 90-91 0.04286731257994711 92-93 0.02793455504459016 94-95 0.015383314874785824 96-97 0.01905213892442094 98-99 0.05016205045641458 100-101 0.06902109337822315 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2330447.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.289317771041354 #Duplication Level Percentage of deduplicated Percentage of total 1 76.82290714351913 40.170174037226744 2 13.05865253487185 13.656560641150579 3 4.3536524762015185 6.829485533783469 4 1.928116617879298 4.032796101276646 5 1.0459845834217416 2.7346910133074878 6 0.6029069468438703 1.8915355757932482 7 0.4033714112514643 1.4764411131876858 8 0.28095747340563476 1.1752859685644907 9 0.21089856533057214 0.9924967890024311 >10 1.1557572792069253 11.08204887131596 >50 0.08777109331040886 3.133714394920391 >100 0.04365396435078206 4.023721059349138 >500 0.0023958061815475052 0.8351095405647271 >1k 0.002065350156506309 2.5236878993031766 >5k 5.782980438217665E-4 2.2154248286705704 >10k+ 3.3045602504100944E-4 3.2268266325832946 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 30431 1.305800990110481 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 18722 0.8033651913130829 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 13564 0.582034262096499 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 11981 0.5141073793997462 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 9550 0.4097926277662612 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 8436 0.3619906395639978 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 7586 0.32551695018166044 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 7174 0.30783793838692747 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 6430 0.2759127326216816 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 6217 0.2667728551646959 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 5892 0.2528270327538022 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 4850 0.20811458059333682 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4540 0.19481241152448434 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3787 0.1625010137540137 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3783 0.16232937286280272 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3747 0.1607846048419037 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3569 0.15314658518301424 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3363 0.1443070792856478 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 3152 0.13525302227426755 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2863 0.12285196788427286 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2697 0.11572887089901637 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2547 0.1092923374786039 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2545 0.1092065170329984 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 8.582044560549973E-5 0.0 12-13 0.0 2.1455111401374928E-5 0.0 1.287306684082496E-4 0.0 14-15 0.0 4.2910222802749856E-5 0.0 1.287306684082496E-4 0.0 16-17 0.0 4.2910222802749856E-5 0.0 2.145511140137493E-4 0.0 18-19 0.0 4.2910222802749856E-5 0.0 2.789164482178741E-4 0.0 20-21 0.0 4.2910222802749856E-5 0.0 3.00371559619249E-4 0.0 22-23 4.2910222802749856E-5 4.2910222802749856E-5 0.0 3.6473689382337377E-4 0.0 24-25 4.2910222802749856E-5 4.2910222802749856E-5 0.0 4.291022280274986E-4 0.0 26-27 4.2910222802749856E-5 4.2910222802749856E-5 0.0 4.5055733942887355E-4 0.0 28-29 4.2910222802749856E-5 4.2910222802749856E-5 0.0 7.938391218508725E-4 0.0 30-31 4.2910222802749856E-5 4.2910222802749856E-5 0.0 0.0013945822410893704 0.0 32-33 4.2910222802749856E-5 4.2910222802749856E-5 0.0 0.0022313315857429928 0.0 34-35 4.2910222802749856E-5 6.436533420412478E-5 0.0 0.002810619593580116 0.0 36-37 4.2910222802749856E-5 8.582044560549971E-5 0.0 0.0037331893838392378 0.0 38-39 4.2910222802749856E-5 8.582044560549971E-5 0.0 0.005084861402125858 0.0 40-41 4.2910222802749856E-5 8.582044560549971E-5 0.0 0.0077452952158963495 0.0 42-43 4.2910222802749856E-5 1.287306684082496E-4 0.0 0.011027927260306714 0.0 44-45 4.2910222802749856E-5 1.501857798096245E-4 0.0 0.018301210025372813 0.0 46-47 4.2910222802749856E-5 1.7164089121099942E-4 0.0 0.035272203143860384 0.0 48-49 4.2910222802749856E-5 1.7164089121099942E-4 0.0 0.046879418412004215 0.0 50-51 4.2910222802749856E-5 1.7164089121099942E-4 0.0 0.06138307371933367 0.0 52-53 4.2910222802749856E-5 1.7164089121099942E-4 0.0 0.08007047574993123 0.0 54-55 4.2910222802749856E-5 1.7164089121099942E-4 0.0 0.09860769200071917 0.0 56-57 4.2910222802749856E-5 1.7164089121099942E-4 0.0 0.12154320608878898 0.0 58-59 4.2910222802749856E-5 1.7164089121099942E-4 0.0 0.15505609009773663 0.0 60-61 4.2910222802749856E-5 1.7164089121099942E-4 0.0 0.19607826309716547 0.0 62-63 4.2910222802749856E-5 1.7164089121099942E-4 0.0 0.2513466300671073 0.0 64-65 4.2910222802749856E-5 1.7164089121099942E-4 0.0 0.2954797942197355 0.0 66-67 4.2910222802749856E-5 1.7164089121099942E-4 0.0 0.3692853774404653 0.0 68-69 4.2910222802749856E-5 1.7164089121099942E-4 0.0 0.4648678987335906 0.0 70-71 4.2910222802749856E-5 1.7164089121099942E-4 0.0 0.5292761431605182 0.0 72-73 4.2910222802749856E-5 1.7164089121099942E-4 0.0 0.631123556982845 0.0 74-75 4.2910222802749856E-5 1.7164089121099942E-4 0.0 0.7149701323394182 0.0 76-77 4.2910222802749856E-5 2.145511140137493E-4 0.0 0.7844846932798729 0.0 78-79 4.2910222802749856E-5 2.145511140137493E-4 0.0 0.8860102804311791 0.0 80-81 4.2910222802749856E-5 2.145511140137493E-4 0.0 0.9918912551969644 0.0 82-83 4.2910222802749856E-5 2.145511140137493E-4 0.0 1.1244409334346588 0.0 84-85 4.2910222802749856E-5 2.145511140137493E-4 0.0 1.3203046454178105 4.2910222802749856E-5 86-87 4.2910222802749856E-5 2.574613368164992E-4 0.0 1.5798471280402429 4.2910222802749856E-5 88-89 4.2910222802749856E-5 2.574613368164992E-4 0.0 1.8968249438841562 4.2910222802749856E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 10465 0.0 25.469284 1 TACATGG 10590 0.0 24.854609 2 ACATGGG 10655 0.0 23.499556 3 GTAAGCG 335 0.0 20.565517 94-95 GAGTACT 5640 0.0 20.173979 12-13 AGTACTT 6095 0.0 19.213583 12-13 CATGGGA 5360 0.0 19.147001 4 CATGGGG 5315 0.0 18.772745 4 AGAGTAC 11580 0.0 18.769484 10-11 GTACTTT 6050 0.0 18.691036 14-15 TAGCACG 135 0.009388495 17.59738 4 GTATACG 135 0.009390817 17.596624 1 GAGTACA 8255 0.0 17.438883 1 GTGTAGC 1255 0.0 17.035774 1 AGTACAT 7980 0.0 16.849098 2 GTATAGG 685 0.0 16.64615 1 TACTTTT 7015 0.0 16.424639 14-15 GTATAAC 695 0.0 16.406637 1 ACTTTTT 7305 0.0 15.707897 16-17 TAGCCCT 1270 0.0 15.338818 4 >>END_MODULE