##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936507_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2330447 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.95271765459588 31.0 31.0 34.0 30.0 34.0 2 32.057288151157266 33.0 31.0 34.0 30.0 34.0 3 32.19855375385065 34.0 31.0 34.0 30.0 34.0 4 35.90024832145936 37.0 35.0 37.0 35.0 37.0 5 35.76851737027274 37.0 35.0 37.0 35.0 37.0 6 35.8320472424389 37.0 35.0 37.0 35.0 37.0 7 35.76222372789426 37.0 35.0 37.0 33.0 37.0 8 35.73204153537927 37.0 35.0 37.0 33.0 37.0 9 37.34817740974157 39.0 37.0 39.0 34.0 39.0 10-11 37.242889025152685 39.0 37.0 39.0 33.0 39.0 12-13 37.13554073531816 39.0 37.0 39.0 33.0 39.0 14-15 38.21358155752952 40.0 38.0 41.0 33.0 41.0 16-17 38.223969693367835 40.0 37.5 41.0 33.0 41.0 18-19 38.378031982705465 40.0 38.0 41.0 33.5 41.0 20-21 38.383050118711125 40.0 38.0 41.0 34.0 41.0 22-23 38.29011816188053 40.0 38.0 41.0 34.0 41.0 24-25 38.16021261157194 40.0 37.5 41.0 33.0 41.0 26-27 37.965780384621496 40.0 37.0 41.0 33.0 41.0 28-29 38.019099125618396 40.0 37.0 41.0 33.0 41.0 30-31 37.945603568757406 40.0 37.0 41.0 33.0 41.0 32-33 37.782429508158735 40.0 37.0 41.0 33.0 41.0 34-35 37.590515682184574 40.0 37.0 41.0 33.0 41.0 36-37 37.418949883863476 39.5 36.5 41.0 32.0 41.0 38-39 37.2394720841109 39.0 36.0 41.0 31.5 41.0 40-41 37.08519631641484 39.0 36.0 41.0 31.0 41.0 42-43 36.8413312982445 39.0 36.0 41.0 31.0 41.0 44-45 36.586387718750956 39.0 35.0 40.0 30.0 41.0 46-47 36.26868407648833 39.0 35.0 40.0 30.0 41.0 48-49 35.89860550357936 39.0 35.0 40.0 29.0 41.0 50-51 35.61104607828455 38.0 35.0 40.0 28.0 41.0 52-53 35.384173937446334 38.0 34.5 40.0 28.0 41.0 54-55 35.08324797774847 38.0 34.0 40.0 27.0 41.0 56-57 34.6962580569307 38.0 34.0 40.0 26.0 41.0 58-59 34.33242377964399 37.0 33.0 40.0 26.0 41.0 60-61 33.89840275277662 37.0 33.0 39.5 25.0 40.5 62-63 33.6037652862305 36.0 32.0 39.0 25.0 40.0 64-65 33.30926684880626 36.0 32.0 39.0 23.5 40.0 66-67 32.822702897770256 35.5 31.5 38.5 22.5 40.0 68-69 32.34268361391613 35.0 31.0 38.0 22.0 40.0 70-71 31.79951635887879 35.0 31.0 37.5 20.5 39.5 72-73 31.22153646918381 34.0 30.0 37.0 19.0 39.0 74-75 30.69746833976486 34.0 29.5 36.0 18.0 39.0 76-77 29.297747599494862 32.5 28.0 34.5 17.0 36.5 78-79 30.10493759351747 34.0 29.5 35.0 17.5 37.0 80-81 29.912191738323163 34.0 29.0 35.0 13.5 37.0 82-83 29.499019715960074 34.0 29.0 35.0 9.0 36.0 84-85 29.04698926858238 33.5 29.0 35.0 7.0 36.0 86-87 28.70273471140944 33.0 29.0 35.0 2.0 35.5 88-89 28.209689600321312 33.0 27.5 35.0 2.0 35.0 90-91 27.605575883081656 32.5 26.5 34.5 2.0 35.0 92-93 27.043711785764707 32.0 25.5 34.0 2.0 35.0 94-95 26.2818379907374 32.0 25.0 34.0 2.0 35.0 96-97 25.437805923069696 32.0 23.5 34.0 2.0 35.0 98-99 24.350320989921677 31.0 10.5 34.0 2.0 35.0 100-101 20.513843266978395 27.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4907135678086725 1101 2 0.5793549721173008 1101 3 0.48023171237296225 1101 4 0.27221170642830117 1101 5 0.34652160233390106 1101 6 0.41096667877040005 1101 7 0.37069325418200094 1101 8 0.3844614160815638 1101 9 0.4936831604979588 1101 10-11 0.4324382292535134 1101 12-13 0.5547895775886147 1101 14-15 0.6561581529868477 1101 16-17 0.5901863558300207 1101 18-19 0.6481879566258826 1101 20-21 0.6478595995918468 1101 22-23 0.6030738674819034 1101 24-25 0.5879921265913524 1101 26-27 0.5409972892197814 1101 28-29 0.48380465530725303 1101 30-31 0.7342781543718573 1101 32-33 0.6136868137409763 1101 34-35 0.7942401429425487 1101 36-37 0.7391617253689517 1101 38-39 0.7927272236497629 1101 40-41 0.7528732877360369 1101 42-43 0.7795679031170337 1101 44-45 0.9323199457227034 1101 46-47 0.903864354666851 1101 48-49 0.7742331047973963 1101 50-51 0.928206551467035 1101 52-53 0.8434544304980918 1101 54-55 0.8803912204276756 1101 56-57 0.9204650069212192 1101 58-59 0.9258293541361482 1101 60-61 0.8684496976983596 1101 62-63 0.7655388990440031 1101 64-65 0.8504182236692799 1101 66-67 0.8942783777896182 1101 68-69 0.7802302382059949 1101 70-71 0.790333728743061 1101 72-73 0.8975799797051955 1101 74-75 1.0497515943443148 1101 76-77 1.057914469466958 1101 78-79 1.036706815921523 1101 80-81 0.9229911145122678 1101 82-83 0.8690638486750153 1101 84-85 0.8871791952704164 1101 86-87 0.8366889304108405 1101 88-89 0.7382326721092092 1101 90-91 0.8998799911626989 1101 92-93 0.9299074242111729 1101 94-95 0.7823176327138803 1101 96-97 0.6778396388272547 1101 98-99 0.5726823895701294 1101 100-101 0.9203988670080143 1102 1 0.3944536842415012 1102 2 0.42216705041278146 1102 3 0.3579655867312894 1102 4 0.21667534066511251 1102 5 0.12268105665249607 1102 6 0.2507734236692585 1102 7 0.26350444244784654 1102 8 0.2045830365118988 1102 9 0.36039846799047837 1102 10-11 0.3616815091059564 1102 12-13 0.34461861561067764 1102 14-15 0.545619651152542 1102 16-17 0.49407014847515285 1102 18-19 0.4823110146659886 1102 20-21 0.43062423930785343 1102 22-23 0.4810175345396246 1102 24-25 0.4415270488112313 1102 26-27 0.5439964771736925 1102 28-29 0.6199757986816934 1102 30-31 0.5897638308282822 1102 32-33 0.49154359695349115 1102 34-35 0.602300166541049 1102 36-37 0.6428203617079333 1102 38-39 0.5843152357134827 1102 40-41 0.5834988471832361 1102 42-43 0.471889744613776 1102 44-45 0.6792206294349867 1102 46-47 0.6716310414510147 1102 48-49 0.6553489858080681 1102 50-51 0.5477125256850002 1102 52-53 0.7368560050876525 1102 54-55 0.8319735023958401 1102 56-57 0.8348280508564727 1102 58-59 0.7695959892138262 1102 60-61 0.6248443487050253 1102 62-63 0.6107228003575145 1102 64-65 0.6105940172366573 1102 66-67 0.7697939592417384 1102 68-69 0.6300627431054053 1102 70-71 0.5475893774644653 1102 72-73 0.33230605401334756 1102 74-75 0.3972639827091662 1102 76-77 0.40888864928345825 1102 78-79 0.43300776799893725 1102 80-81 0.5499241741120464 1102 82-83 0.3946300558603504 1102 84-85 0.29865748363323874 1102 86-87 0.42297889274139777 1102 88-89 0.43012401474857853 1102 90-91 0.38895540718798216 1102 92-93 0.49022674565257773 1102 94-95 0.5469974000516551 1102 96-97 0.6634567599458805 1102 98-99 0.4133827666036325 1102 100-101 0.47931014037764896 1103 1 0.3102135138733537 1103 2 0.3810554464212075 1103 3 0.38633420745340885 1103 4 0.2332452787114292 1103 5 0.28245114483166134 1103 6 0.22781766529637792 1103 7 0.3083542429307684 1103 8 0.37138373615007936 1103 9 0.35836578384638784 1103 10-11 0.36368643140961865 1103 12-13 0.4091325555372407 1103 14-15 0.4450628819700526 1103 16-17 0.4215394049732595 1103 18-19 0.35301059115363387 1103 20-21 0.3568008288921263 1103 22-23 0.4488885277547823 1103 24-25 0.4460553134494418 1103 26-27 0.3509085983116407 1103 28-29 0.40978742215631314 1103 30-31 0.4396783462508296 1103 32-33 0.5060693082984145 1103 34-35 0.47731009214528797 1103 36-37 0.5454063522673138 1103 38-39 0.503335340261188 1103 40-41 0.6071144219140336 1103 42-43 0.7069276549492045 1103 44-45 0.7409516189923622 1103 46-47 0.612010548794764 1103 48-49 0.6561575618485378 1103 50-51 0.5734988972283759 1103 52-53 0.6542019413014941 1103 54-55 0.6847854982174297 1103 56-57 0.6581702062857602 1103 58-59 0.5618163254883157 1103 60-61 0.503015044420728 1103 62-63 0.7411843454323019 1103 64-65 0.6611544979483455 1103 66-67 0.5875594674137687 1103 68-69 0.7368155769524734 1103 70-71 0.49842047144218427 1103 72-73 0.8410574020546839 1103 74-75 0.715224022773782 1103 76-77 0.6841579441108188 1103 78-79 0.5749906758596772 1103 80-81 0.7725986125019553 1103 82-83 0.670961166455367 1103 84-85 0.9214389493907511 1103 86-87 0.8153486255292037 1103 88-89 0.7188448665574683 1103 90-91 0.6717463696840227 1103 92-93 0.8977477764905117 1103 94-95 0.8410881281342775 1103 96-97 1.243905498752337 1103 98-99 1.1985980641538454 1103 100-101 0.8620173596303147 1104 1 0.21886245653462666 1104 2 0.33701430732931925 1104 3 0.2007947849851277 1104 4 0.1588518318371399 1104 5 0.09959167221091292 1104 6 0.10043575532270665 1104 7 0.16378102442943998 1104 8 0.20979677474318947 1104 9 0.24843002368309897 1104 10-11 0.22574217354317483 1104 12-13 0.27186955839793114 1104 14-15 0.2609854289309155 1104 16-17 0.28096403248306245 1104 18-19 0.19410292471579993 1104 20-21 0.2614218011322009 1104 22-23 0.1570936686256985 1104 24-25 0.2555856851229166 1104 26-27 0.2627494186835193 1104 28-29 0.429441167322139 1104 30-31 0.4660918406393648 1104 32-33 0.539603670614234 1104 34-35 0.48629633464529576 1104 36-37 0.3984779100903708 1104 38-39 0.49865352045492983 1104 40-41 0.5267272465302852 1104 42-43 0.5889848827213697 1104 44-45 0.6172940964874627 1104 46-47 0.5140274435368966 1104 48-49 0.387261744969031 1104 50-51 0.4989885077684022 1104 52-53 0.5271796531673161 1104 54-55 0.4021372332618327 1104 56-57 0.5755612153281362 1104 58-59 0.6933801958800103 1104 60-61 0.4774970495763071 1104 62-63 0.48266161656489714 1104 64-65 0.5041570184820614 1104 66-67 0.5993446605364952 1104 68-69 0.6120296988268592 1104 70-71 0.502297258488607 1104 72-73 0.5898836259336839 1104 74-75 0.7622288612030985 1104 76-77 0.588609489932761 1104 78-79 0.709716580214117 1104 80-81 0.6941100233852495 1104 82-83 0.525462978878334 1104 84-85 0.5902694281087904 1104 86-87 0.61056400851243 1104 88-89 0.46426076397580474 1104 90-91 0.5904465743616143 1104 92-93 0.5891831634666254 1104 94-95 0.688193462344028 1104 96-97 0.8089961161586707 1104 98-99 0.792637584760346 1104 100-101 0.43019563551490236 1105 1 0.11939719176808339 1105 2 0.25439714984253214 1105 3 0.17779433244093923 1105 4 0.13967051387621154 1105 5 0.19479724146497546 1105 6 0.22006937075601485 1105 7 0.1764201888157615 1105 8 0.1328297230615192 1105 9 0.24469142363770402 1105 10-11 0.18046634363211211 1105 12-13 0.09544182378338206 1105 14-15 0.20128677965548292 1105 16-17 0.2107823240089246 1105 18-19 0.09916327803861691 1105 20-21 0.09256598720822495 1105 22-23 0.07098339164327427 1105 24-25 0.16963912058179176 1105 26-27 0.2941082812891338 1105 28-29 0.22350959595055997 1105 30-31 0.17838035377089767 1105 32-33 0.26645792107559174 1105 34-35 -0.07926362534472986 1105 36-37 0.15067701402468003 1105 38-39 0.14816307040607768 1105 40-41 0.06610370126247034 1105 42-43 0.14491012763799205 1105 44-45 0.22654113556556865 1105 46-47 0.3255123030437801 1105 48-49 0.22473969930013737 1105 50-51 0.4353582927605757 1105 52-53 0.27515730227779756 1105 54-55 0.2570206044481651 1105 56-57 0.36235210607888746 1105 58-59 0.3412222227140447 1105 60-61 0.2836355259826817 1105 62-63 0.3278611060781387 1105 64-65 0.18911921130536058 1105 66-67 0.14370112790044232 1105 68-69 0.27611406166297314 1105 70-71 0.138095230639518 1105 72-73 0.22439577695656254 1105 74-75 0.3719747914774558 1105 76-77 0.27335413376921025 1105 78-79 0.21369317127347642 1105 80-81 0.5292697461067739 1105 82-83 0.659995483816143 1105 84-85 0.5487060289239345 1105 86-87 0.6798963155150233 1105 88-89 0.5963423403521517 1105 90-91 0.684254067562879 1105 92-93 0.9066827370036457 1105 94-95 0.8496403631212495 1105 96-97 0.666861115601801 1105 98-99 0.5720912856836193 1105 100-101 0.3360511132027817 1106 1 0.12587715711528702 1106 2 0.17787301022824664 1106 3 0.2150980526544899 1106 4 0.13449371622895256 1106 5 0.17256326268310573 1106 6 0.10964459910371716 1106 7 0.13458572296364935 1106 8 0.1323516378698173 1106 9 0.13815805087742916 1106 10-11 0.1240579066689591 1106 12-13 0.19044365431994237 1106 14-15 0.33111490367064533 1106 16-17 0.2824280501418812 1106 18-19 0.20268967598519794 1106 20-21 0.1529010256897223 1106 22-23 0.1255595319066387 1106 24-25 0.23247999934663 1106 26-27 0.1737815975642789 1106 28-29 0.32114022451524704 1106 30-31 0.2537674485551449 1106 32-33 0.18824715658183067 1106 34-35 0.14810938369530646 1106 36-37 0.27368622169067436 1106 38-39 0.38068471499232004 1106 40-41 0.3160274289054499 1106 42-43 0.3280200248731475 1106 44-45 0.3469160971779388 1106 46-47 0.4953467031843246 1106 48-49 0.5484122585511741 1106 50-51 0.26894558789805245 1106 52-53 0.4095163395630834 1106 54-55 0.3186393653693571 1106 56-57 0.4058057251100351 1106 58-59 0.10770040446151796 1106 60-61 0.08213695018036304 1106 62-63 0.26801739568109184 1106 64-65 0.15934323746660795 1106 66-67 0.07035218136418564 1106 68-69 -0.010315711444370379 1106 70-71 0.06286336267417525 1106 72-73 0.09331251168164201 1106 74-75 0.14350396339099092 1106 76-77 0.059637145451116425 1106 78-79 0.2586349394242866 1106 80-81 0.15374422118532394 1106 82-83 0.19167181276557343 1106 84-85 0.2695825609087059 1106 86-87 0.1679012720448121 1106 88-89 0.24740988513995177 1106 90-91 0.41022902944346384 1106 92-93 0.3496648755263152 1106 94-95 0.3297813881949061 1106 96-97 0.5010488669653164 1106 98-99 0.619040274060719 1106 100-101 0.20243652764833087 1107 1 0.06862238005446031 1107 2 0.14781553769480382 1107 3 0.0325284274291775 1107 4 0.012117209160571463 1107 5 -0.07360363670296266 1107 6 0.013959930974273504 1107 7 -0.010266756356344331 1107 8 0.0458187014516227 1107 9 -0.055246686992084904 1107 10-11 0.025179908106373716 1107 12-13 0.08897240452888155 1107 14-15 0.11754352333857554 1107 16-17 0.15785987587359074 1107 18-19 0.107311172359978 1107 20-21 0.12095990404932877 1107 22-23 0.19768935855095293 1107 24-25 0.17603604647033677 1107 26-27 0.052842750035487995 1107 28-29 0.012922749880004858 1107 30-31 0.13088934425741172 1107 32-33 -0.03091698450302971 1107 34-35 0.013699297980529934 1107 36-37 0.21435711765583676 1107 38-39 0.20393640606967978 1107 40-41 0.16722769208281107 1107 42-43 0.23442249765722778 1107 44-45 0.23268466195708015 1107 46-47 0.17737137332208874 1107 48-49 0.10588019831631357 1107 50-51 0.049383811734522 1107 52-53 0.2017714333458045 1107 54-55 0.3788399784720369 1107 56-57 0.353465859461366 1107 58-59 0.3563960824244887 1107 60-61 0.27719089847748535 1107 62-63 -0.06894851488792142 1107 64-65 0.017790468454876418 1107 66-67 0.13077038966596888 1107 68-69 0.029567151418596893 1107 70-71 -0.0834505504756855 1107 72-73 0.30523774403704707 1107 74-75 0.28294565601730426 1107 76-77 0.06413451585342855 1107 78-79 0.26538184123131003 1107 80-81 0.39650575570535196 1107 82-83 0.3213018960916436 1107 84-85 0.26140565568946883 1107 86-87 0.2660223616777522 1107 88-89 0.3737193045459932 1107 90-91 0.2750863732250224 1107 92-93 0.17994803457663977 1107 94-95 0.4085115219547042 1107 96-97 0.4980927958900203 1107 98-99 0.6659678217902254 1107 100-101 0.3096987110050051 1108 1 0.050827610795646905 1108 2 0.18246119280596673 1108 3 -0.022675087260886073 1108 4 0.08427760899432002 1108 5 0.08170080637344057 1108 6 0.03483341884541602 1108 7 0.06125069695617924 1108 8 0.07442334478928103 1108 9 0.07338526972482384 1108 10-11 -0.008781411788582716 1108 12-13 0.04826622808760561 1108 14-15 0.045201771513092126 1108 16-17 0.020003927172822955 1108 18-19 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2316 82-83 -2.016540299700754 2316 84-85 -1.8095119812001705 2316 86-87 -2.0893015217401683 2316 88-89 -2.348232977583507 2316 90-91 -2.4883031738943657 2316 92-93 -2.4309491385631894 2316 94-95 -2.457558354994635 2316 96-97 -2.485680993306193 2316 98-99 -1.9086060213166185 2316 100-101 -1.6272580689006801 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 7.0 4 37.0 5 61.0 6 117.0 7 318.0 8 707.0 9 1297.0 10 2319.0 11 3624.0 12 5180.0 13 7449.0 14 9627.0 15 12684.0 16 15808.0 17 20136.0 18 18479.0 19 14156.0 20 13653.0 21 14651.0 22 16220.0 23 18073.0 24 21106.0 25 25532.0 26 31317.0 27 39135.0 28 48127.0 29 60388.0 30 73810.0 31 91649.0 32 114679.0 33 146925.0 34 192557.0 35 263048.0 36 368080.0 37 445621.0 38 224977.0 39 8889.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.06913353907054 18.951555624792665 14.72643259660186 22.252878239534933 2 15.473178762047853 21.4526559456387 38.095011258753054 24.9791540335604 3 21.7827598799638 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22-23 22.41289938324872 26.01007879638025 28.455006507363212 23.122015313007818 24-25 23.581907599304337 26.117633731355966 28.19930339245386 22.101155276885837 26-27 22.906590801327646 25.38287751910043 29.620909311311788 22.08962236826014 28-29 21.74337948331756 27.921684485151978 28.47793018071656 21.857005850813902 30-31 22.369314550275732 25.98686038455935 28.201126528605087 23.442698536559828 32-33 22.42487283647136 26.32715769264521 28.62664676420931 22.621322706674125 34-35 23.85550967934618 25.851797053414266 28.165065099685638 22.127628167553915 36-37 23.10431832956791 25.362349624423118 29.25513266950307 22.2781993765059 38-39 22.370580514817817 26.58547465272466 28.97156377054734 22.07238106191018 40-41 22.69643978172428 26.773876427998577 28.214737344380715 22.31494644589643 42-43 22.13228608245985 26.938331690567235 27.251344071602574 23.67803815537034 44-45 22.9786487184103 25.676787628868635 27.615505710890115 23.72905794183095 46-47 24.00041708736564 26.893960686512074 27.363913446647793 21.741708779474493 48-49 23.556203417351778 26.63524432061263 27.395371011255143 22.413181250780447 50-51 21.79339577423268 27.037349967838374 28.705356165870743 22.4638980920582 52-53 23.22806880531301 27.713956713148065 26.529411625877707 22.528562855661217 54-55 22.362205265012072 26.44791264829275 27.391639608125672 23.798242478569506 56-57 23.46228246070855 26.247257457302165 27.117661384891527 23.17279869709776 58-59 24.703403603891612 26.055469077700266 26.58975561036261 22.651371708045513 60-61 23.064604132520618 26.698384097457506 28.519404326223803 21.717607443798077 62-63 22.41673467951573 26.825318973825567 28.162447295405762 22.595499051252936 64-65 22.10668059288261 28.104551323669945 27.20604863504918 22.582719448398265 66-67 22.86601248012166 26.729490659647638 26.516911500300804 23.8875853599299 68-69 22.451914608214043 26.722555222543313 27.27726412266675 23.548266046575893 70-71 23.6908958115017 26.548383193138836 26.86960324142376 22.891117753935703 72-73 23.234092348570282 25.75129120129352 28.35939766253517 22.655218787601033 74-75 22.270051528197822 28.376534554361015 26.81702071963532 22.53639319780584 76-77 22.8059790260353 26.505387671627307 26.86680429606254 23.821829006274854 78-79 22.975771894436292 26.793258949156318 27.196020065318066 23.034949091089327 80-81 23.826282744328243 26.54437497331449 26.824908078473747 22.804434203883524 82-83 23.250049348174976 26.206219586505203 27.90954694084227 22.634184124477553 84-85 22.635971467522488 27.168997385424028 27.440903845997056 22.754127301056428 86-87 22.709735971473073 27.403955493192246 26.826873152165053 23.059435383169628 88-89 22.342988561383677 27.502735050767136 25.959370415880052 24.194905971969135 90-91 22.9498544694976 26.161824769044756 26.69405980214718 24.194260959310466 92-93 23.83897215575749 26.851276523434684 26.918389695810834 22.391361624996996 94-95 23.067370541734206 26.4299223427411 27.256764570525 23.245942544999696 96-97 21.591130956646438 26.571149777102466 28.50192002121485 23.33579924503624 98-99 22.46014466012252 27.43723470538459 26.348722208967622 23.753898425525268 100-101 21.8364330468951 26.33044020059187 27.03853645945863 24.7945902930544 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 160.0 1 120.5 2 126.0 3 173.0 4 146.5 5 135.0 6 241.5 7 498.5 8 785.0 9 991.0 10 1080.5 11 1033.5 12 1045.0 13 1223.5 14 1441.5 15 1613.5 16 1765.0 17 1956.0 18 2175.0 19 2434.0 20 2748.0 21 3162.0 22 3678.0 23 4445.0 24 5475.5 25 6956.5 26 9067.0 27 11816.0 28 14742.0 29 18308.5 30 22905.5 31 27815.5 32 33776.5 33 38568.0 34 42038.5 35 45782.5 36 51496.0 37 57116.0 38 59314.5 39 60769.0 40 63144.5 41 66105.0 42 67798.0 43 70343.0 44 73628.5 45 76879.0 46 80499.0 47 105229.0 48 154408.0 49 149287.0 50 102177.0 51 81463.5 52 75318.5 53 73813.0 54 72939.5 55 68738.0 56 63350.0 57 58049.0 58 53364.0 59 47670.5 60 39904.5 61 33056.0 62 27351.0 63 21518.5 64 15934.5 65 12101.0 66 9188.0 67 7099.0 68 5300.5 69 3681.5 70 2741.5 71 2110.5 72 1628.5 73 1404.5 74 1200.5 75 989.5 76 709.0 77 421.0 78 279.5 79 201.0 80 137.0 81 88.5 82 65.0 83 38.0 84 17.0 85 11.5 86 7.0 87 5.5 88 3.5 89 1.5 90 0.5 91 0.0 92 0.0 93 0.0 94 1.5 95 1.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012443964612797459 2 0.011156657928714963 3 0.0064365334204124795 4 3.4328178242199885E-4 5 0.005535418741554732 6 0.0 7 4.720124508302484E-4 8 0.0019738702489264935 9 4.2910222802749856E-5 10-11 0.0 12-13 0.0 14-15 8.796595674563721E-4 16-17 0.004097926277662612 18-19 0.0019953253603278683 20-21 4.5055733942887355E-4 22-23 4.2910222802749863E-4 24-25 4.2910222802749863E-4 26-27 8.582044560549971E-5 28-29 8.582044560549971E-5 30-31 5.792880078371231E-4 32-33 0.0034971831584241133 34-35 9.011146788577471E-4 36-37 0.0013731271296879954 38-39 0.005471053407350607 40-41 0.0 42-43 0.001201486238476996 44-45 0.005084861402125858 46-47 0.0 48-49 0.0019738702489264935 50-51 0.0012873066840824957 52-53 7.080186762453727E-4 54-55 2.574613368164992E-4 56-57 0.011349753931327337 58-59 0.002124056028736118 60-61 0.003325542267213114 62-63 2.1455111401374931E-4 64-65 0.00300371559619249 66-67 9.011146788577471E-4 68-69 0.00152331290949762 70-71 0.004848855176710734 72-73 0.0025317031453622415 74-75 0.0072732827650661015 76-77 0.0014589475752934953 78-79 0.006758360091433102 80-81 0.002681888925171866 82-83 0.0028749849277842406 84-85 0.002145511140137493 86-87 7.294737876467476E-4 88-89 0.0022098764743416176 90-91 0.0077023849930936 92-93 0.002360062254151242 94-95 6.22198230639873E-4 96-97 1.7164089121099942E-4 98-99 8.152942332522473E-4 100-101 0.0018665946919196189 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2330447.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.887844467306806 #Duplication Level Percentage of deduplicated Percentage of total 1 74.9322232506444 37.38207099117659 2 14.103461656840588 14.071826031741772 3 4.680706630029905 7.005310930680711 4 2.0920278500128715 4.174670400108655 5 1.0950161985872997 2.7313998902152377 6 0.6957448600712257 2.0825526820896867 7 0.45512960671481895 1.5893804522578783 8 0.3190115444605515 1.273183865065866 9 0.22869434407622335 1.0268161081044642 >10 1.2455948667368784 11.490043227214919 >50 0.09856400071853091 3.3969982508419845 >100 0.0476847882337396 4.2524628589050275 >500 0.00285399006777382 0.9562719094363228 >1k 0.0020756291401991095 2.192470373123786 >5k 7.78360927574666E-4 2.6526578927974773 >10k+ 4.3242273754148114E-4 3.7218841362395754 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 34875 1.4964940202459012 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 16551 0.7102070976083129 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 13017 0.5585623702233949 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 11814 0.5069413721916869 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 10007 0.4294025995871178 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 8894 0.38164352160765724 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 8713 0.3738767712803595 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 8003 0.3434105130904071 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 7118 0.3054349659099735 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 6328 0.27153588989580113 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 6300 0.27033440365732414 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5679 0.24368715529681645 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 5271 0.2261797843932945 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5176 0.22210331322703325 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 4249 0.18232553668888415 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3725 0.15984057994024323 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3446 0.14786862777827603 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3426 0.147010423322221 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 3176 0.13628286762153355 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 3072 0.13182020445004758 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2839 0.12182212253700686 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2806 0.1204060851845161 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2784 0.11946206028285561 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2734 0.11731654914271812 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 4.2910222802749856E-5 7 0.0 0.0 0.0 0.0 4.2910222802749856E-5 8 0.0 0.0 0.0 0.0 4.2910222802749856E-5 9 0.0 0.0 0.0 4.2910222802749856E-5 4.2910222802749856E-5 10-11 0.0 0.0 0.0 8.582044560549971E-5 4.2910222802749856E-5 12-13 0.0 0.0 0.0 8.582044560549971E-5 4.2910222802749856E-5 14-15 0.0 2.1455111401374928E-5 0.0 8.582044560549971E-5 4.2910222802749856E-5 16-17 0.0 4.2910222802749856E-5 0.0 1.287306684082496E-4 8.582044560549971E-5 18-19 0.0 4.2910222802749856E-5 0.0 2.574613368164992E-4 8.582044560549971E-5 20-21 0.0 4.2910222802749856E-5 0.0 3.8619200522474874E-4 8.582044560549971E-5 22-23 0.0 4.2910222802749856E-5 0.0 4.5055733942887355E-4 8.582044560549971E-5 24-25 0.0 4.2910222802749856E-5 0.0 5.149226736329984E-4 8.582044560549971E-5 26-27 0.0 4.2910222802749856E-5 0.0 5.363777850343732E-4 8.582044560549971E-5 28-29 0.0 4.2910222802749856E-5 0.0 8.796595674563721E-4 8.582044560549971E-5 30-31 0.0 4.2910222802749856E-5 0.0 0.0014804026866948703 8.582044560549971E-5 32-33 0.0 4.2910222802749856E-5 0.0 0.0022956969199471175 8.582044560549971E-5 34-35 0.0 8.582044560549971E-5 0.0 0.00291789515058699 8.582044560549971E-5 36-37 0.0 8.582044560549971E-5 0.0 0.0038190098294447377 8.582044560549971E-5 38-39 0.0 8.582044560549971E-5 0.0 0.005149226736329983 8.582044560549971E-5 40-41 0.0 8.582044560549971E-5 0.0 0.00783111566150185 8.582044560549971E-5 42-43 0.0 8.582044560549971E-5 0.0 0.011070837483109464 8.582044560549971E-5 44-45 0.0 8.582044560549971E-5 0.0 0.01845139580518244 8.582044560549971E-5 46-47 0.0 8.582044560549971E-5 0.0 0.03578712581749338 8.582044560549971E-5 48-49 0.0 8.582044560549971E-5 0.0 0.04765180242245372 8.582044560549971E-5 50-51 0.0 1.0727555700687464E-4 0.0 0.06269183551481754 8.582044560549971E-5 52-53 0.0 1.287306684082496E-4 0.0 0.08125050687700686 8.582044560549971E-5 54-55 0.0 1.287306684082496E-4 0.0 0.09983063335059755 8.582044560549971E-5 56-57 0.0 1.287306684082496E-4 0.0 0.12268032699306186 8.582044560549971E-5 58-59 0.0 1.287306684082496E-4 0.0 0.15625757633621362 8.582044560549971E-5 60-61 0.0 1.287306684082496E-4 0.0 0.19775176178647272 8.582044560549971E-5 62-63 0.0 1.287306684082496E-4 0.0 0.25381396787826543 8.582044560549971E-5 64-65 0.0 1.287306684082496E-4 0.0 0.29745366446866206 1.287306684082496E-4 66-67 0.0 1.287306684082496E-4 0.0 0.37160252947181377 1.287306684082496E-4 68-69 0.0 1.287306684082496E-4 0.0 0.46733523654474873 1.287306684082496E-4 70-71 0.0 1.287306684082496E-4 0.0 0.5313572889664515 1.287306684082496E-4 72-73 0.0 1.287306684082496E-4 0.0 0.6328614210063563 1.287306684082496E-4 74-75 0.0 1.287306684082496E-4 0.0 0.7172443741479639 1.287306684082496E-4 76-77 0.0 1.287306684082496E-4 0.0 0.7870163964252352 1.287306684082496E-4 78-79 0.0 1.287306684082496E-4 0.0 0.8884347080195345 1.287306684082496E-4 80-81 0.0 1.7164089121099942E-4 0.0 0.9944229583423265 1.287306684082496E-4 82-83 0.0 1.7164089121099942E-4 0.0 1.1268009956888099 1.287306684082496E-4 84-85 0.0 1.7164089121099942E-4 0.0 1.3226432525605603 1.287306684082496E-4 86-87 0.0 1.7164089121099942E-4 0.0 1.5819282738461764 1.287306684082496E-4 88-89 0.0 1.7164089121099942E-4 0.0 1.8954947269772708 1.287306684082496E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGCG 350 0.0 25.104837 94-95 GTACATG 10380 0.0 24.89842 1 TACATGG 10665 0.0 23.963728 2 ACATGGG 10445 0.0 23.42091 3 CATGGGG 5280 0.0 20.690054 4 GAGTACT 7595 0.0 20.41978 12-13 AACGTCG 70 4.8574535E-4 20.355494 22-23 GTACTTT 7960 0.0 19.543121 14-15 AGTACTT 8070 0.0 19.423883 12-13 GTGTAGC 1370 0.0 19.419483 1 AGAGTAC 13625 0.0 18.965227 10-11 ACCGTCC 255 2.6806447E-7 18.62554 8 CATGGGA 4915 0.0 17.781239 4 AGACCCG 440 5.456968E-12 17.271698 5 TACTTTT 9045 0.0 17.251326 14-15 GTATAAC 580 0.0 17.201311 1 CATGGGT 1640 0.0 17.087397 4 GAGTACA 8430 0.0 16.906897 1 TAAGGTG 935 0.0 16.763706 5 ACACCGT 400 4.3291948E-10 16.623297 6 >>END_MODULE