##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936506_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2344469 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.360151061924896 31.0 31.0 34.0 30.0 34.0 2 31.38988274103859 31.0 31.0 34.0 28.0 34.0 3 31.45603290126677 31.0 31.0 34.0 28.0 34.0 4 35.07142683481846 37.0 35.0 37.0 32.0 37.0 5 34.99586302911235 37.0 35.0 37.0 32.0 37.0 6 35.1318102308028 37.0 35.0 37.0 32.0 37.0 7 34.966928118904534 37.0 35.0 37.0 32.0 37.0 8 34.95360868495169 37.0 35.0 37.0 32.0 37.0 9 36.44326540466093 39.0 37.0 39.0 32.0 39.0 10-11 36.31524643746623 39.0 35.0 39.0 32.0 39.0 12-13 36.24015459364146 39.0 35.0 39.0 32.0 39.0 14-15 37.29537093474045 40.0 37.0 41.0 32.0 41.0 16-17 37.256235847008426 39.0 37.0 41.0 31.5 41.0 18-19 37.22054823501612 39.5 36.5 41.0 31.0 41.0 20-21 37.106486159552546 39.0 36.0 41.0 31.0 41.0 22-23 37.00762219504715 39.0 36.0 41.0 31.0 41.0 24-25 36.84691714840333 39.0 36.0 41.0 30.5 41.0 26-27 36.63981481520976 39.0 36.0 40.5 30.0 41.0 28-29 36.80338362332793 39.0 36.0 41.0 30.0 41.0 30-31 36.782035718962376 39.0 36.0 41.0 30.0 41.0 32-33 36.6560323467702 39.0 36.0 41.0 30.0 41.0 34-35 36.46605947871352 39.0 35.0 41.0 30.0 41.0 36-37 36.23654012912945 39.0 35.0 40.0 29.5 41.0 38-39 36.0546394514067 38.5 35.0 40.0 29.0 41.0 40-41 35.982198101147844 38.5 35.0 40.0 29.0 41.0 42-43 35.73117324221391 38.0 35.0 40.0 28.0 41.0 44-45 35.48255703103773 38.0 34.5 40.0 27.0 41.0 46-47 35.17958373516562 38.0 34.0 40.0 26.0 41.0 48-49 34.94046050512931 38.0 34.0 40.0 25.5 41.0 50-51 34.231076205315574 37.5 33.5 39.5 24.5 40.5 52-53 34.43833486388603 38.0 33.5 39.5 25.5 40.5 54-55 34.80454635996466 38.0 34.0 40.0 26.0 41.0 56-57 34.67751482318597 38.0 34.0 40.0 25.5 41.0 58-59 34.43709982942833 38.0 34.0 40.0 25.0 41.0 60-61 34.04790807641304 37.5 33.0 40.0 23.0 41.0 62-63 33.76625751929328 37.0 33.0 40.0 23.0 41.0 64-65 33.46961657415815 36.5 32.5 39.5 22.5 41.0 66-67 33.02957855275544 36.0 32.0 39.0 22.0 41.0 68-69 32.625892472879784 35.5 32.0 39.0 21.0 40.0 70-71 32.17720046628895 35.0 31.0 38.0 20.0 40.0 72-73 31.75472591021677 35.0 31.0 37.0 19.0 39.5 74-75 31.351827641994838 35.0 31.0 37.0 18.0 39.0 76-77 30.9489620037629 35.0 30.5 36.0 16.5 39.0 78-79 30.482982500515043 34.0 30.0 36.0 12.0 38.5 80-81 30.0152418308794 34.0 29.5 35.5 7.5 37.0 82-83 29.59538919047341 34.0 29.0 35.0 7.0 37.0 84-85 29.20975986460047 34.0 29.0 35.0 2.0 36.0 86-87 28.952686514515655 34.0 29.0 35.0 2.0 36.0 88-89 28.565185762746275 34.0 29.0 35.0 2.0 36.0 90-91 28.18154132129706 34.0 27.5 35.0 2.0 35.0 92-93 27.812931414320257 34.0 27.0 35.0 2.0 35.0 94-95 27.371092132162975 33.0 26.0 35.0 2.0 35.0 96-97 26.799853826175564 33.0 25.0 35.0 2.0 35.0 98-99 26.181496535036292 33.0 24.5 35.0 2.0 35.0 100-101 24.085546449963722 31.0 12.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3720239260261984 1101 2 0.48001581497233303 1101 3 0.4139994855121074 1101 4 0.3915693380258034 1101 5 0.5627601280029779 1101 6 0.49626157899288614 1101 7 0.48672361757930815 1101 8 0.5213351714192669 1101 9 0.7447393053217226 1101 10-11 0.5569372384357933 1101 12-13 0.7061289032449878 1101 14-15 0.7802881788180187 1101 16-17 0.7641007044877952 1101 18-19 0.9128082257582051 1101 20-21 0.8560188706675049 1101 22-23 0.8380707840617916 1101 24-25 0.8135427206202408 1101 26-27 0.8073790875378322 1101 28-29 0.8171057569564155 1101 30-31 0.6548616847226043 1101 32-33 0.6063763918693681 1101 34-35 0.7048394223981305 1101 36-37 0.8132278029179574 1101 38-39 0.7538045847477193 1101 40-41 0.7583029221080224 1101 42-43 0.44973507004655744 1101 44-45 0.503115588634607 1101 46-47 0.5019818552837023 1101 48-49 0.5221210985873199 1101 50-51 0.6201043207077177 1101 52-53 0.6875739343748606 1101 54-55 0.7730729453142757 1101 56-57 0.7049470684153576 1101 58-59 0.8313664518000721 1101 60-61 0.6575431689370177 1101 62-63 0.7763936808490257 1101 64-65 0.8526294739951723 1101 66-67 0.8674076195135783 1101 68-69 0.5885763062064484 1101 70-71 0.6110902722825955 1101 72-73 0.6051696379097287 1101 74-75 0.5430326150933276 1101 76-77 0.489912774133618 1101 78-79 0.4787867703297124 1101 80-81 0.49202992234899057 1101 82-83 0.48974243506854265 1101 84-85 0.6472004312464925 1101 86-87 0.5824891211542393 1101 88-89 0.4925888272726944 1101 90-91 0.3849689024777838 1101 92-93 0.2662657475613308 1101 94-95 0.18888637878100667 1101 96-97 0.3389226668952041 1101 98-99 0.3827886722166447 1101 100-101 0.6651578916478798 1102 1 0.3902071976393984 1102 2 0.4042673654118083 1102 3 0.47986713672643333 1102 4 0.5371712208616586 1102 5 0.46590785476869456 1102 6 0.5164637681985553 1102 7 0.3320366055773434 1102 8 0.466465601675516 1102 9 0.7587527499000188 1102 10-11 0.7616510206157727 1102 12-13 0.8308100778609031 1102 14-15 0.8568824916424234 1102 16-17 0.6912254014642585 1102 18-19 0.8399524426561342 1102 20-21 0.518102073868036 1102 22-23 0.310112679390393 1102 24-25 0.5811422536293591 1102 26-27 0.7075105216757578 1102 28-29 0.6469543195465732 1102 30-31 0.5219170766826267 1102 32-33 0.5621907258124637 1102 34-35 0.5893279632747053 1102 36-37 0.2922699138952183 1102 38-39 0.41512000437025875 1102 40-41 0.1950509403712246 1102 42-43 0.3735227899710125 1102 44-45 0.3722038409881492 1102 46-47 0.6634850379604202 1102 48-49 0.7412638208169824 1102 50-51 0.6902124424822844 1102 52-53 0.5912905274235314 1102 54-55 0.5608765265692028 1102 56-57 0.7822013404895998 1102 58-59 0.7742864833293979 1102 60-61 0.24553432734210645 1102 62-63 0.46694157715920426 1102 64-65 0.7586373340168819 1102 66-67 0.5917510065359934 1102 68-69 0.6002231926860304 1102 70-71 0.6797204571455495 1102 72-73 0.35971127297271366 1102 74-75 0.27557065105460055 1102 76-77 0.5317662743343945 1102 78-79 0.500305159954781 1102 80-81 0.5680888316129575 1102 82-83 0.6328383317951456 1102 84-85 0.4460188253332582 1102 86-87 0.48099017708904057 1102 88-89 0.2950295610730187 1102 90-91 0.24838894144326318 1102 92-93 0.21633378843046813 1102 94-95 0.4580949110316368 1102 96-97 0.25769004892051584 1102 98-99 0.3338252729989293 1102 100-101 0.542417554938531 1103 1 0.3751057220540517 1103 2 0.4534993162566643 1103 3 0.5083466321414107 1103 4 0.4249278191021233 1103 5 0.5529231692033605 1103 6 0.3476384179215941 1103 7 0.3970042544159611 1103 8 0.19094891406205505 1103 9 0.5285172148257757 1103 10-11 0.46412739728570074 1103 12-13 0.5936789741975446 1103 14-15 0.6631793310037821 1103 16-17 0.728192421899017 1103 18-19 0.5355280140381566 1103 20-21 0.5123923266260277 1103 22-23 0.5193443974930787 1103 24-25 0.6680935639677656 1103 26-27 0.5015017007475819 1103 28-29 0.693284557409072 1103 30-31 0.4753436232543322 1103 32-33 0.656237181149244 1103 34-35 0.5468490264409311 1103 36-37 0.6250487390127475 1103 38-39 0.6072189896144025 1103 40-41 0.4891648909367916 1103 42-43 0.532976194988926 1103 44-45 0.7200777412814077 1103 46-47 0.7011537760323918 1103 48-49 0.9152554653731357 1103 50-51 0.30689307367904206 1103 52-53 0.5593629567862806 1103 54-55 0.7337562454166644 1103 56-57 0.6353177581221416 1103 58-59 0.7251220277133257 1103 60-61 0.8450740797044389 1103 62-63 0.8441887027934243 1103 64-65 0.7904432968712314 1103 66-67 0.690392955984386 1103 68-69 0.6111655657251731 1103 70-71 0.6061238924199586 1103 72-73 0.5031584172263059 1103 74-75 0.5132690637530111 1103 76-77 0.44523331804337474 1103 78-79 0.6379058209425388 1103 80-81 0.868001553299873 1103 82-83 0.8014169753737903 1103 84-85 0.5878775042887092 1103 86-87 0.6363100811992624 1103 88-89 0.8055396155346841 1103 90-91 0.9279541790730157 1103 92-93 0.8922694119467387 1103 94-95 0.642360958604133 1103 96-97 0.7425687902508713 1103 98-99 0.7874071749679281 1103 100-101 0.8556041443347979 1104 1 0.35713042517967253 1104 2 0.4657635046676418 1104 3 0.42573413760231205 1104 4 0.39012103567149126 1104 5 0.40060179027647536 1104 6 0.3573687487528403 1104 7 0.48683705158820345 1104 8 0.4939156720746851 1104 9 0.5761640633900527 1104 10-11 0.5767351259484741 1104 12-13 0.5577283804487934 1104 14-15 0.6134990418739434 1104 16-17 0.30594372258610036 1104 18-19 0.49644187082357405 1104 20-21 0.5827902772992033 1104 22-23 0.535631779835505 1104 24-25 0.5151863794092009 1104 26-27 0.4311255936742313 1104 28-29 0.39359036780662393 1104 30-31 0.3257864525962475 1104 32-33 0.30894578752756985 1104 34-35 0.41377583052369715 1104 36-37 0.6054014138608395 1104 38-39 0.4553205822114137 1104 40-41 0.26693207787828754 1104 42-43 0.31156946716018297 1104 44-45 0.14557120805928037 1104 46-47 0.49209463945031473 1104 48-49 0.3004415331280086 1104 50-51 0.27307719536775465 1104 52-53 0.37126771869105113 1104 54-55 0.586220600633311 1104 56-57 0.5634721725150911 1104 58-59 0.49107182714936215 1104 60-61 0.47472378779452384 1104 62-63 0.39972307667642326 1104 64-65 0.35642884037971356 1104 66-67 0.4275449477235398 1104 68-69 0.4821652082844494 1104 70-71 0.5729950946122528 1104 72-73 0.2716103017332365 1104 74-75 0.10361022106664564 1104 76-77 0.25032486258445985 1104 78-79 0.4093979373887855 1104 80-81 0.6085313598847257 1104 82-83 0.3239119116301943 1104 84-85 0.43556208270723573 1104 86-87 0.49761712278153425 1104 88-89 0.3966750855692389 1104 90-91 0.3522045464426533 1104 92-93 0.3607451258352974 1104 94-95 0.2086384120375513 1104 96-97 0.36833033757205413 1104 98-99 0.36414175537223414 1104 100-101 0.4981634199215925 1105 1 0.3143619767830863 1105 2 0.22397277784760306 1105 3 0.15893024146427592 1105 4 0.349735866832809 1105 5 0.3597391803173693 1105 6 0.27498805014228367 1105 7 0.42085595190662417 1105 8 0.06430879200645734 1105 9 0.4200030928446026 1105 10-11 0.35279322047874473 1105 12-13 0.24854693336349953 1105 14-15 0.47153840268662606 1105 16-17 0.3774618248933521 1105 18-19 0.18509077219690084 1105 20-21 -0.08980690680530046 1105 22-23 0.048014438054245545 1105 24-25 -0.014379550310515299 1105 26-27 0.029832491256023275 1105 28-29 0.13229087105721504 1105 30-31 0.1696842873657758 1105 32-33 0.08899248697044726 1105 34-35 0.01453468366813837 1105 36-37 0.0020200485055497097 1105 38-39 0.047321198771065554 1105 40-41 -0.18085520358186358 1105 42-43 -0.1914132169363043 1105 44-45 -0.07165869801950464 1105 46-47 -0.08635165072805506 1105 48-49 0.13962999246972885 1105 50-51 0.1427760541178955 1105 52-53 0.2252010346587454 1105 54-55 0.25125460188349535 1105 56-57 0.36115782063841806 1105 58-59 0.2282614861536416 1105 60-61 0.3105561620919701 1105 62-63 0.16290451420264418 1105 64-65 0.018983715383001254 1105 66-67 -0.11249780177742963 1105 68-69 0.06479188210220599 1105 70-71 0.2501141428571927 1105 72-73 0.22443503936252895 1105 74-75 0.22574006934722846 1105 76-77 0.25509389681512573 1105 78-79 0.1953755241142474 1105 80-81 0.23815064966673916 1105 82-83 0.3701774412689254 1105 84-85 0.32632749746649736 1105 86-87 0.20210846619535516 1105 88-89 0.355651251749471 1105 90-91 0.3947510879902687 1105 92-93 0.21057837137317748 1105 94-95 0.34717682086985135 1105 96-97 0.5168409841505728 1105 98-99 0.5221700720517433 1105 100-101 0.6522959911699111 1106 1 -0.005798288190796086 1106 2 -0.0010329146421277358 1106 3 -0.0017593658263237444 1106 4 0.08613311969649118 1106 5 0.24157658438410579 1106 6 0.2675326983464217 1106 7 0.30782432930355696 1106 8 0.2889079485966519 1106 9 0.24215984229476817 1106 10-11 0.3945887633580014 1106 12-13 0.33660275727406486 1106 14-15 0.37108759369500177 1106 16-17 0.23859542993064053 1106 18-19 0.2573898338903291 1106 20-21 0.3646191191261323 1106 22-23 0.32522248243065377 1106 24-25 0.11829766051430113 1106 26-27 0.24735626151371548 1106 28-29 0.10451908922238573 1106 30-31 0.24531411364283429 1106 32-33 0.20255839744633874 1106 34-35 0.21795901238211002 1106 36-37 0.29546517948546125 1106 38-39 0.29515780588923946 1106 40-41 0.18257168510725563 1106 42-43 0.23175700956556256 1106 44-45 0.19023144877434106 1106 46-47 0.28739665962253724 1106 48-49 0.31441854773557765 1106 50-51 0.5193989895209725 1106 52-53 0.4598200425729573 1106 54-55 0.5118058619309096 1106 56-57 0.5542246149309236 1106 58-59 0.4411958922196675 1106 60-61 0.4433238604703007 1106 62-63 0.4702232791495433 1106 64-65 0.35632559249629736 1106 66-67 0.3901056896601389 1106 68-69 0.4285936360471325 1106 70-71 0.5108583059164289 1106 72-73 0.6831865348750092 1106 74-75 0.6037012334054381 1106 76-77 0.5824396138911503 1106 78-79 0.5960082624453023 1106 80-81 0.5365136146575971 1106 82-83 0.7222452628419447 1106 84-85 0.7091874108880099 1106 86-87 0.7086881452573905 1106 88-89 0.620170884587175 1106 90-91 0.7390141935494299 1106 92-93 0.7662054303763277 1106 94-95 0.8615317663601516 1106 96-97 0.8070855703625099 1106 98-99 1.0163227663433254 1106 100-101 1.1565604227830342 1107 1 0.425243358248526 1107 2 0.5645836434155918 1107 3 0.49950639008624975 1107 4 0.5331198638838686 1107 5 0.6061257097060775 1107 6 0.5182073398226663 1107 7 0.7690356569979855 1107 8 0.7237589991812001 1107 9 0.8150054157899973 1107 10-11 0.8714474898633426 1107 12-13 0.8638436378346768 1107 14-15 1.0360788412034694 1107 16-17 1.0693842821406108 1107 18-19 1.0976811362268037 1107 20-21 1.1613093066264923 1107 22-23 1.1827985277322952 1107 24-25 1.0845816495943907 1107 26-27 1.0396505708236319 1107 28-29 0.8764281604016944 1107 30-31 0.871693380660929 1107 32-33 0.9587040600370784 1107 34-35 0.8397974782259752 1107 36-37 0.9467558507287421 1107 38-39 1.139723622341073 1107 40-41 0.9768577930613134 1107 42-43 1.1219785632541743 1107 44-45 1.1358428524283966 1107 46-47 1.167016347445582 1107 48-49 1.1236499276308365 1107 50-51 1.1219577011770596 1107 52-53 1.2284048089542452 1107 54-55 1.1972626620314273 1107 56-57 1.1994656439840625 1107 58-59 1.246081367367161 1107 60-61 1.2634940700160868 1107 62-63 1.4064871503494203 1107 64-65 1.2882203569928734 1107 66-67 1.359520157971268 1107 68-69 1.2270821535778182 1107 70-71 1.336548796113064 1107 72-73 1.2669120407490233 1107 74-75 1.087934969191565 1107 76-77 1.2987607261000882 1107 78-79 1.3131732119292892 1107 80-81 1.3015563348120267 1107 82-83 1.197768270614958 1107 84-85 1.2582192111313084 1107 86-87 1.2059399628628924 1107 88-89 1.1844074617204718 1107 90-91 1.3039914484939459 1107 92-93 1.3317652328812777 1107 94-95 1.3737202890309597 1107 96-97 1.4643291494890285 1107 98-99 1.504696087949558 1107 100-101 1.8425565791113883 1108 1 -0.005296197644991452 1108 2 -0.12055743104289363 1108 3 -0.2714526154693395 1108 4 -0.10535342787241575 1108 5 0.1653808127312928 1108 6 0.034115811667355445 1108 7 0.2202384217395803 1108 8 -0.0018345803987571685 1108 9 0.19895308885589458 1108 10-11 -0.06408443348203718 1108 12-13 0.14755346764872002 1108 14-15 0.19956717106573763 1108 16-17 0.24248245827372017 1108 18-19 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-4.079770456697304 2316 92-93 -4.290823299064751 2316 94-95 -4.327132196295114 2316 96-97 -4.696363132310495 2316 98-99 -5.1234982901253545 2316 100-101 -5.639212587157061 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4625.0 3 1993.0 4 1910.0 5 2143.0 6 2402.0 7 3005.0 8 3515.0 9 4519.0 10 5754.0 11 7162.0 12 8651.0 13 10269.0 14 11488.0 15 13703.0 16 15839.0 17 18720.0 18 17270.0 19 14319.0 20 15245.0 21 16480.0 22 18685.0 23 21110.0 24 24317.0 25 28068.0 26 33143.0 27 39058.0 28 46888.0 29 56086.0 30 68179.0 31 83382.0 32 104363.0 33 132721.0 34 173340.0 35 233733.0 36 321760.0 37 412154.0 38 319382.0 39 49072.0 40 16.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.33101891055188 18.243484500292432 14.053719464827413 22.371777124328272 2 15.30967079242553 20.761371430911453 38.81101191999645 25.117945856666562 3 21.476947516104083 23.711193325373536 29.13707870344895 25.67478045507343 4 12.628175807371047 15.85778800301751 38.166053379847554 33.347982809763884 5 13.470437325149312 38.11851697598561 33.935328928390376 14.475716770474707 6 31.755228930842307 35.94298303643756 16.94410039683793 15.357687635882206 7 28.72382364812225 32.50556571468555 20.523556903406156 18.247053733786043 8 26.343069657746078 32.42015561224163 19.629481254130397 21.607293475881896 9 25.504233819276557 18.082881241038677 21.274893871893745 35.13799106779102 10-11 25.61101939801358 25.646886722624814 26.81499651035042 21.927097369011186 12-13 27.541049503488658 23.297606820027386 26.786094524698832 22.375249151785123 14-15 23.36783518463571 25.15650194188902 26.70845597904931 24.767206894425957 16-17 22.19376778950157 28.48171863730738 26.846561729183815 22.47795184400723 18-19 21.954636509673662 28.432864637000343 27.947656198333004 21.66484265499299 20-21 23.493325665214957 26.02342075443363 27.352314770570807 23.130938809780606 22-23 22.558739852745628 26.166361006558148 27.943626293509638 23.331272847186582 24-25 23.751032781563875 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27.06341836688993 22.719707461930255 50-51 21.73644412737919 27.168731678551282 28.48232023519702 22.612503958872512 52-53 23.119271963998294 27.87459886553083 26.261466202334926 22.74466296813595 54-55 22.238454736664302 26.565889055763876 27.18922471947016 24.00643148810166 56-57 23.397222282057314 26.373668559611996 26.760354912853213 23.46875424547748 58-59 24.57447679755401 26.22990537483237 26.416967524424077 22.778650303189547 60-61 23.117749491084787 26.720773231219724 28.278133760726142 21.88334351696935 62-63 22.293194268880452 27.162568617469034 27.840836263507292 22.703400850143225 64-65 22.052200018171217 28.308942243257988 26.891928610153613 22.746929128417182 66-67 22.72038010513546 26.961564645001456 26.170254785438008 24.147800464425075 68-69 22.350504733812365 26.868773474441255 27.068576827673873 23.712144964072507 70-71 23.688848458607367 26.624015800514243 26.561692497848437 23.125443243029952 72-73 23.096542945939326 25.959449164507593 28.192974134584787 22.751033754968294 74-75 22.12596163135931 28.49883882300225 26.74669748635945 22.628502059279 76-77 22.63548300410768 26.76357176067122 26.667235400253368 23.933709834967733 78-79 22.9366431725712 26.973677894436364 26.99810572321627 23.09157320977617 80-81 23.765253082294276 26.796532206954062 26.52194846093805 22.916266249813614 82-83 23.148347402762898 26.51336565594694 27.72123014751627 22.61705679377389 84-85 22.732217103812246 27.360818611920774 27.202773070063447 22.704191214203536 86-87 22.702531729263526 27.68088975453944 26.761783006944118 22.85479550925292 88-89 22.306652649907555 27.667081922752985 25.776610994198144 24.24965443314132 90-91 22.882790070686 26.45695491367947 26.502983998600783 24.157271017033747 92-93 23.84325347351905 27.06129378146292 26.82956587064233 22.265886874375703 94-95 23.056833363761854 26.64200961313693 27.135420843478542 23.165736179622677 96-97 21.61029718664109 26.88768851825129 28.459455072869304 23.042559222238314 98-99 22.46236238935259 27.740779169361566 26.299333769068383 23.497524672217455 100-101 21.90396781218483 26.589575273347016 27.091347092409606 24.415109822058547 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 157.0 1 113.0 2 102.5 3 135.5 4 127.5 5 142.5 6 219.0 7 416.0 8 751.5 9 951.5 10 925.5 11 928.5 12 1014.0 13 1132.5 14 1286.0 15 1409.5 16 1523.0 17 1658.5 18 1834.5 19 2047.0 20 2337.0 21 2652.0 22 3127.5 23 3927.0 24 4934.0 25 6322.5 26 8500.0 27 11320.0 28 14129.5 29 17637.0 30 22415.0 31 27472.5 32 33655.5 33 38603.5 34 42168.0 35 46366.0 36 52095.0 37 57436.0 38 59551.5 39 61437.5 40 63611.5 41 66547.0 42 68979.5 43 71660.0 44 75086.5 45 79227.5 46 83766.5 47 106648.5 48 152091.0 49 147673.0 50 103610.0 51 83241.0 52 76605.0 53 74826.0 54 73608.0 55 69237.0 56 64017.0 57 58438.5 58 53732.5 59 48542.5 60 40740.0 61 33435.5 62 27817.5 63 22106.0 64 16225.5 65 12345.5 66 9343.0 67 7136.0 68 5343.5 69 3790.0 70 2851.5 71 2204.5 72 1672.0 73 1363.5 74 1162.0 75 923.5 76 672.5 77 424.0 78 273.5 79 181.0 80 128.5 81 89.5 82 59.0 83 39.0 84 23.5 85 18.0 86 18.0 87 12.5 88 10.0 89 8.5 90 6.0 91 3.0 92 1.0 93 1.5 94 2.5 95 1.5 96 1.0 97 1.0 98 0.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.013990374792756912 2 0.007379069631545565 3 0.008018873356824083 4 0.034677361910095635 5 0.044402378534329094 6 0.0617197326985343 7 0.027938096003828586 8 0.024141927233842717 9 0.015696518060166288 10-11 0.04736680246145289 12-13 0.0434213461555687 14-15 0.020836274653237046 16-17 0.024120600443000097 18-19 0.03130772895696211 20-21 0.024099273652157485 22-23 0.015440596570054883 24-25 0.0030710578813368827 26-27 0.002559214901114069 28-29 0.0017061432674093794 30-31 0.00695253381469322 32-33 0.0014288949864553551 34-35 0.0016634896857241448 36-37 0.005672926364136186 38-39 0.0066752855337391965 40-41 0.004180051005152979 42-43 6.611305161211346E-4 44-45 0.001322261032242269 46-47 0.002985750717966414 48-49 9.383787970751587E-4 50-51 0.002196659456789576 52-53 0.0010450127512882448 54-55 0.002815136391225476 56-57 0.0026658488553271548 58-59 0.0026018684827993034 60-61 9.170520062325414E-4 62-63 0.0017274700582519966 64-65 0.004350665331893917 66-67 0.0027724828095402414 68-69 0.006995187396378456 70-71 0.010300839976984126 72-73 0.004393318913579152 74-75 0.0034336133256613758 76-77 0.003369632953133524 78-79 0.03542379958958724 80-81 0.023779371789518226 82-83 0.0035615740707170794 84-85 0.008871944990528772 86-87 0.007762951866712675 88-89 0.005651599573293569 90-91 0.01275342092388511 92-93 0.01452354456382234 94-95 0.007549683958286504 96-97 0.0042866849593660656 98-99 0.022904973364970917 100-101 0.04152326177057577 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2344469.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.50538682843686 #Duplication Level Percentage of deduplicated Percentage of total 1 76.95143823882442 40.40365031734044 2 13.011309841643396 13.663277127602683 3 4.360739197298378 6.868868952362357 4 1.918292728586048 4.028828070583524 5 1.0011050299603348 2.6281703426980623 6 0.5787696866215466 1.8233115768382486 7 0.4071877388265901 1.4965684817220644 8 0.2961693335502077 1.2440388339819197 9 0.20693669499022618 0.977876209751407 >10 1.1326394760927088 10.992587524362097 >50 0.08709182658190665 3.142310248582835 >100 0.043009398521636766 4.041025755602949 >500 0.0024511423858392173 0.8740751333262656 >1k 0.0019609139086712345 2.4565479440323537 >5k 5.719332233624434E-4 2.171095382699708 >10k+ 3.268189847785391E-4 3.1877680985131085 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 29892 1.2750008637350292 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 18803 0.802015296427464 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 13735 0.5858469444466956 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 11878 0.5066392432572152 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 9243 0.3942470555166223 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 8539 0.3642189340102172 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 7413 0.3161910010326432 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 7077 0.3018593975864044 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 6380 0.272129851151796 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 6142 0.2619782987107102 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 5815 0.24803057749963853 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4742 0.2022632843513819 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 4681 0.1996614158685826 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3750 0.1599509313196293 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3633 0.15496046226245686 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3625 0.154619233608975 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3540 0.15099367916573006 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3311 0.14122600895981136 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 3147 0.1342308215634329 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2794 0.11917410722854513 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2708 0.11550589920361497 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2668 0.1137997559362056 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2544 0.10851071180723651 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 4.2653581685234485E-5 0.0 4 0.0 0.0 0.0 4.2653581685234485E-5 0.0 5 0.0 0.0 0.0 4.2653581685234485E-5 0.0 6 0.0 0.0 0.0 4.2653581685234485E-5 0.0 7 0.0 0.0 0.0 4.2653581685234485E-5 0.0 8 0.0 4.2653581685234485E-5 0.0 4.2653581685234485E-5 0.0 9 0.0 4.2653581685234485E-5 0.0 8.530716337046897E-5 0.0 10-11 0.0 4.2653581685234485E-5 0.0 1.0663395421308621E-4 0.0 12-13 0.0 4.2653581685234485E-5 0.0 1.2796074505570345E-4 0.0 14-15 0.0 4.2653581685234485E-5 0.0 1.2796074505570345E-4 0.0 16-17 0.0 4.2653581685234485E-5 0.0 1.2796074505570345E-4 0.0 18-19 0.0 4.2653581685234485E-5 0.0 1.919411175835552E-4 0.0 20-21 0.0 4.2653581685234485E-5 0.0 2.559214901114069E-4 0.0 22-23 0.0 4.2653581685234485E-5 0.0 2.559214901114069E-4 0.0 24-25 0.0 4.2653581685234485E-5 0.0 3.8388223516711034E-4 0.0 26-27 0.0 4.2653581685234485E-5 0.0 4.052090260097276E-4 0.0 28-29 0.0 4.2653581685234485E-5 0.0 5.544965619080483E-4 0.0 30-31 0.0 4.2653581685234485E-5 0.0 0.0010876663329734792 0.0 32-33 0.0 8.530716337046897E-5 0.0 0.001684816476566762 0.0 34-35 0.0 8.530716337046897E-5 0.0 0.0022393130384748103 0.0 36-37 0.0 8.530716337046897E-5 0.0 0.003156365044707352 0.0 38-39 0.0 8.530716337046897E-5 0.0 0.004265358168523448 0.0 40-41 0.0 8.530716337046897E-5 0.0 0.0060568085993032966 2.1326790842617242E-5 42-43 0.0 1.2796074505570345E-4 0.0 0.008807964618000921 4.2653581685234485E-5 44-45 0.0 1.2796074505570345E-4 0.0 0.015739171641851523 4.2653581685234485E-5 46-47 0.0 1.2796074505570345E-4 0.0 0.03132905574780473 4.2653581685234485E-5 48-49 0.0 1.2796074505570345E-4 0.0 0.04150193497973315 4.2653581685234485E-5 50-51 0.0 1.2796074505570345E-4 0.0 0.05483117925636893 4.2653581685234485E-5 52-53 0.0 1.2796074505570345E-4 0.0 0.07018646866305334 4.2653581685234485E-5 54-55 0.0 1.2796074505570345E-4 0.0 0.08799433901663874 4.2653581685234485E-5 56-57 0.0 1.2796074505570345E-4 0.0 0.10895857441493148 4.2653581685234485E-5 58-59 0.0 1.2796074505570345E-4 0.0 0.14126866254149661 4.2653581685234485E-5 60-61 0.0 1.2796074505570345E-4 0.0 0.17880381442450294 4.2653581685234485E-5 62-63 0.0 1.2796074505570345E-4 0.0 0.23049995542700713 4.2653581685234485E-5 64-65 0.0 1.2796074505570345E-4 0.0 0.26946400229646883 4.2653581685234485E-5 66-67 0.0 1.2796074505570345E-4 0.0 0.33852015104486344 4.2653581685234485E-5 68-69 0.0 1.2796074505570345E-4 0.0 0.4270050062508824 4.2653581685234485E-5 70-71 0.0 1.2796074505570345E-4 0.0 0.4845446879442637 4.2653581685234485E-5 72-73 0.0 1.2796074505570345E-4 0.0 0.5802166716642446 8.530716337046897E-5 74-75 0.0 1.2796074505570345E-4 0.0 0.6590831442002432 8.530716337046897E-5 76-77 0.0 1.2796074505570345E-4 0.0 0.7264118228903857 8.530716337046897E-5 78-79 0.0 1.2796074505570345E-4 0.0 0.8156217889850538 8.530716337046897E-5 80-81 0.0 1.2796074505570345E-4 0.0 0.9098435509277367 8.530716337046897E-5 82-83 0.0 1.2796074505570345E-4 0.0 1.033410977069861 8.530716337046897E-5 84-85 0.0 1.2796074505570345E-4 0.0 1.2209801025306797 8.530716337046897E-5 86-87 0.0 1.2796074505570345E-4 0.0 1.4790769253080334 8.530716337046897E-5 88-89 0.0 1.2796074505570345E-4 0.0 1.7926660578578775 8.530716337046897E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 10580 0.0 23.626503 1 TACATGG 10790 0.0 22.549107 2 GTGTAGC 1220 0.0 22.20314 1 ACATGGG 10810 0.0 21.628193 3 GAGTACT 5930 0.0 20.914976 12-13 GTACTTT 6145 0.0 20.065498 14-15 GTACCTG 1935 0.0 18.173983 1 TACCTGG 1865 0.0 18.090906 2 CATGGGA 5235 0.0 17.792192 4 AGTACTT 6365 0.0 17.58183 12-13 CATGGGG 5470 0.0 17.375319 4 TAGCCCT 1395 0.0 17.37345 4 GTATATG 800 0.0 17.22691 1 AGAGTAC 11945 0.0 17.147457 10-11 ACTTTTT 7335 0.0 17.133501 16-17 CCTATAC 650 0.0 16.81494 3 GAGTACA 8590 0.0 16.375626 1 GTAGCCC 1220 0.0 16.359509 3 AGTACAT 8180 0.0 15.975712 2 GGTAAGG 855 0.0 15.562263 3 >>END_MODULE