FastQCFastQC Report
Thu 26 May 2016
SRR936506_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936506_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2344469
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT342141.4593496437786126No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT161120.687234508112498No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT126030.5375630899790101No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC116930.49874833064544677No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT96710.41250278847790267No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC91170.3888727042242828No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC86990.37104350707985473No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT78730.33581164860785107No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG71450.30475984114100035No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC61330.2615944164755431No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA61230.26116788065869073No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58280.24858507406154656No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50820.21676550212436163No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA50600.21582712332728649No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC41050.17509295281788753No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA36230.15453392644560454No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC34970.14915957515326497No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT34140.14561932787339052No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT32030.13661942213780603No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29440.1255721444813303No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG29190.12450580493919945No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG27800.11857695708495186No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC27170.11588978143878209No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC25800.11004624074790496No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG104350.023.1847521
TACATGG107500.022.0515522
ACATGGG106050.021.5896873
GAGTACT75300.021.50524312-13
GTACTTT78900.020.43351614-15
TAAGGTG9000.020.0511785
GTGTAAG10150.019.8631271
TATCACG1200.00476155919.7941022
AGTACTT79700.019.42424612-13
ACCGTAC1250.00567709519.2010231
AGAGTAC136300.018.41359910-11
CATGGGA52400.017.9483854
ACTTTTT91600.017.83396316-17
CATGGGG51850.017.0394544
GAGTACA86750.016.9876481
GTGTAGC13350.016.8997761
TACTTTT91200.016.74042314-15
GTAAGGT8900.016.5448634
AGGTAAG9550.016.4156322
AGTACAT83750.016.2228932
Produced by FastQC (version 0.11.5)