##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936506_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2344469 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.533545549120078 31.0 31.0 34.0 30.0 34.0 2 31.73737080763277 31.0 31.0 34.0 30.0 34.0 3 32.033478369728925 33.0 31.0 34.0 30.0 34.0 4 35.80681808972522 37.0 35.0 37.0 35.0 37.0 5 35.62462672784328 37.0 35.0 37.0 33.0 37.0 6 35.73683721132589 37.0 35.0 37.0 33.0 37.0 7 35.675330746535785 37.0 35.0 37.0 33.0 37.0 8 35.66089122952788 37.0 35.0 37.0 33.0 37.0 9 37.29569211621054 39.0 37.0 39.0 34.0 39.0 10-11 37.180964431604764 39.0 37.0 39.0 33.0 39.0 12-13 37.069133351731246 39.0 37.0 39.0 33.0 39.0 14-15 38.17986844782337 40.0 37.0 41.0 33.0 41.0 16-17 38.12041895201003 40.0 37.0 41.0 33.0 41.0 18-19 38.18737483839624 40.0 37.5 41.0 33.5 41.0 20-21 38.258310730489505 40.0 38.0 41.0 33.5 41.0 22-23 38.17980894607692 40.0 37.5 41.0 33.0 41.0 24-25 38.0604128269557 40.0 37.0 41.0 33.0 41.0 26-27 37.86009603880453 40.0 37.0 41.0 33.0 41.0 28-29 37.814286731878305 40.0 37.0 41.0 33.0 41.0 30-31 37.86809422517423 40.0 37.0 41.0 33.0 41.0 32-33 37.723092734431546 40.0 37.0 41.0 33.0 41.0 34-35 37.54597821510969 40.0 37.0 41.0 32.0 41.0 36-37 37.32274877594884 39.5 36.0 41.0 31.5 41.0 38-39 37.19087627091678 39.0 36.0 41.0 31.0 41.0 40-41 37.03013731467552 39.0 36.0 41.0 31.0 41.0 42-43 36.81050719800518 39.0 36.0 41.0 31.0 41.0 44-45 36.569122475067914 39.0 35.0 40.5 30.0 41.0 46-47 36.241197900249475 39.0 35.0 40.0 30.0 41.0 48-49 35.83541177127955 39.0 35.0 40.0 28.5 41.0 50-51 35.65035088968973 38.0 35.0 40.0 28.0 41.0 52-53 35.42518412484874 38.0 34.5 40.0 28.0 41.0 54-55 35.18252405981909 38.0 34.0 40.0 27.0 41.0 56-57 34.86417606716063 38.0 34.0 40.0 26.5 41.0 58-59 34.49687818435646 37.5 33.5 40.0 26.0 41.0 60-61 34.059427529218766 37.0 33.0 40.0 25.5 41.0 62-63 33.81509117842889 36.5 33.0 39.0 25.0 40.5 64-65 33.39808694420783 36.0 32.0 39.0 24.5 40.0 66-67 32.95090679381984 35.5 32.0 39.0 23.0 40.0 68-69 32.47420012804605 35.0 31.0 38.0 22.0 40.0 70-71 31.95957101586756 35.0 31.0 37.5 21.5 39.5 72-73 31.52918848575093 34.5 31.0 37.0 20.5 39.0 74-75 31.18160124957933 34.0 30.0 36.0 20.0 39.0 76-77 29.73376252789011 32.5 28.5 35.0 18.5 37.0 78-79 30.56287457842266 34.0 30.0 35.0 19.0 37.0 80-81 30.368277422307568 34.0 30.0 35.0 18.0 37.0 82-83 30.02051445337942 34.0 29.5 35.0 16.0 36.5 84-85 29.565052257035603 34.0 29.0 35.0 10.5 36.0 86-87 29.270692638716913 34.0 29.0 35.0 7.0 36.0 88-89 28.865022527489167 34.0 29.0 35.0 4.5 35.0 90-91 28.389771201922485 33.0 28.5 35.0 2.0 35.0 92-93 27.953600580771166 33.0 27.0 35.0 2.0 35.0 94-95 27.407246800874738 33.0 26.5 34.5 2.0 35.0 96-97 26.72486797650129 32.5 25.0 34.0 2.0 35.0 98-99 25.909951891025216 32.0 24.5 34.0 2.0 35.0 100-101 23.06726277890644 29.5 10.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.43240472070201363 1101 2 0.3746456932520488 1101 3 0.2783850420136389 1101 4 0.21300161552751717 1101 5 0.2926513745439152 1101 6 0.23184658974957983 1101 7 0.2702461771674791 1101 8 0.27946503754722585 1101 9 0.3889288289522099 1101 10-11 0.2997524947513739 1101 12-13 0.34917560638435674 1101 14-15 0.3907729924607679 1101 16-17 0.3554936590318718 1101 18-19 0.38661527144584795 1101 20-21 0.31578628759213956 1101 22-23 0.2323001938694489 1101 24-25 0.1584130350407733 1101 26-27 0.2711163833318224 1101 28-29 0.21125065436075374 1101 30-31 0.1840286122371424 1101 32-33 0.39404889204834603 1101 34-35 0.40194505533824554 1101 36-37 0.37395035524856013 1101 38-39 0.4006187807973731 1101 40-41 0.458231127713681 1101 42-43 0.5718981226525628 1101 44-45 0.45961090892402723 1101 46-47 0.5027770269537868 1101 48-49 0.5624198519321055 1101 50-51 0.7224116357199435 1101 52-53 0.5820454266166522 1101 54-55 0.4694001195106239 1101 56-57 0.37019313532753273 1101 58-59 0.5004666535031674 1101 60-61 0.4507019257197342 1101 62-63 0.35169031485025926 1101 64-65 0.4892894761693256 1101 66-67 0.22545600821747058 1101 68-69 0.1502654969246393 1101 70-71 0.10056660445505017 1101 72-73 0.2099568451404572 1101 74-75 0.1506788638626162 1101 76-77 0.5192407227292613 1101 78-79 0.5489258905573315 1101 80-81 0.41238080953522527 1101 82-83 0.48240656505427637 1101 84-85 0.587868151910012 1101 86-87 0.5216867919169914 1101 88-89 0.4548450900613332 1101 90-91 0.3332034422285659 1101 92-93 0.4005629937824082 1101 94-95 0.2652045556998104 1101 96-97 0.025365879284635184 1101 98-99 0.11812929618857382 1101 100-101 0.28352126503554587 1102 1 0.5829776768732806 1102 2 0.6322751939882281 1102 3 0.36090783384575786 1102 4 0.255679600030561 1102 5 0.254014943306089 1102 6 0.22949676301212207 1102 7 0.3085256726983445 1102 8 0.18403121194006644 1102 9 0.25409636564965865 1102 10-11 0.35923062500315694 1102 12-13 0.4191405820681169 1102 14-15 0.4473984256171235 1102 16-17 0.5502151004572298 1102 18-19 0.40618724604392753 1102 20-21 0.4233958965988194 1102 22-23 0.23133999963693697 1102 24-25 0.26645301183994974 1102 26-27 0.17448068827388852 1102 28-29 0.3299959138083963 1102 30-31 0.3060448528118087 1102 32-33 0.5032210242930262 1102 34-35 0.6489315512706639 1102 36-37 0.644314280946439 1102 38-39 0.5242077693308147 1102 40-41 0.5653369368423071 1102 42-43 0.5285016191352483 1102 44-45 0.4264484213795967 1102 46-47 0.7114301151912699 1102 48-49 0.5652409453451526 1102 50-51 0.5729987205962814 1102 52-53 0.38598454677013194 1102 54-55 0.607643921470796 1102 56-57 0.7090515359581175 1102 58-59 0.5825832450646118 1102 60-61 0.6876245078521279 1102 62-63 0.5937782400127318 1102 64-65 0.5343544682126407 1102 66-67 0.6398462802322129 1102 68-69 0.5844424626736853 1102 70-71 0.4644039979320489 1102 72-73 0.4967825712815319 1102 74-75 0.029484319030004258 1102 76-77 0.37045244509277 1102 78-79 0.4667656115525851 1102 80-81 0.5886577842076584 1102 82-83 0.6064222032999353 1102 84-85 0.6554823713362374 1102 86-87 0.6409944110071066 1102 88-89 0.6151938088108686 1102 90-91 0.6580660591621914 1102 92-93 0.6852285152803468 1102 94-95 0.5721646160814338 1102 96-97 0.6107980689364751 1102 98-99 0.2522869279201565 1102 100-101 -0.0016508449006522596 1103 1 0.5055175921551331 1103 2 0.5393202994204316 1103 3 0.3838678487412608 1103 4 0.27278498568433207 1103 5 0.1959678828396747 1103 6 0.13387697061813242 1103 7 0.14793474033321985 1103 8 0.23647616841728336 1103 9 0.21474897468936405 1103 10-11 0.29269196652901996 1103 12-13 0.3569390738988645 1103 14-15 0.3966425954258028 1103 16-17 0.33190178295036077 1103 18-19 0.31847835994794593 1103 20-21 0.3331472115920704 1103 22-23 0.3404175260531659 1103 24-25 0.3164181005147171 1103 26-27 0.2815787425493639 1103 28-29 0.31887491115513455 1103 30-31 0.41922736922013115 1103 32-33 0.3330015484283848 1103 34-35 0.3245459905947854 1103 36-37 0.25897936496637186 1103 38-39 0.3390846487238264 1103 40-41 0.469215003220377 1103 42-43 0.3851434987610034 1103 44-45 0.43038234587803714 1103 46-47 0.5918111141206523 1103 48-49 0.5809097299655477 1103 50-51 0.26160948259414596 1103 52-53 0.24324784798504595 1103 54-55 0.3303839950173142 1103 56-57 0.35186911264666065 1103 58-59 0.158802232170693 1103 60-61 0.21285666816493887 1103 62-63 0.19958748453165498 1103 64-65 0.23349681332272354 1103 66-67 0.2709970738830165 1103 68-69 0.16824942631936324 1103 70-71 0.22139731823181918 1103 72-73 0.3049716044705697 1103 74-75 0.35418757276551815 1103 76-77 -0.05767095480482354 1103 78-79 0.10989389903571123 1103 80-81 -0.015393675085736191 1103 82-83 0.2659303027273907 1103 84-85 0.4733866444824457 1103 86-87 0.30854200678050603 1103 88-89 0.3190323090203364 1103 90-91 0.1973837754960357 1103 92-93 0.24011260968056902 1103 94-95 0.39440138468916786 1103 96-97 0.2288739894317473 1103 98-99 0.10173416527061718 1103 100-101 0.11989118833493251 1104 1 0.3574219569035364 1104 2 0.3619939560390435 1104 3 0.16704033374860927 1104 4 0.04355427755454144 1104 5 0.06467593319910492 1104 6 0.12123151210111871 1104 7 0.07919888902856087 1104 8 0.13721090768412836 1104 9 0.08336567908588677 1104 10-11 0.08780297173176876 1104 12-13 0.12354815289326382 1104 14-15 0.2285203506980622 1104 16-17 0.030875133758577533 1104 18-19 0.12063491898523893 1104 20-21 0.03729550977706708 1104 22-23 0.20220711261340085 1104 24-25 0.18328334536160895 1104 26-27 0.2378498414810153 1104 28-29 0.10694433408694692 1104 30-31 0.10487810991674706 1104 32-33 0.03793820198889364 1104 34-35 0.15677522924603693 1104 36-37 0.10805796221010411 1104 38-39 0.12391526153609078 1104 40-41 0.19336608149989587 1104 42-43 0.17334398329176537 1104 44-45 0.10688458981146454 1104 46-47 0.13856436087390733 1104 48-49 0.2999950788674184 1104 50-51 0.19944855483688428 1104 52-53 0.07742712436616017 1104 54-55 0.0856165300663676 1104 56-57 0.1473752884280657 1104 58-59 0.2145411415793319 1104 60-61 0.17581190864311935 1104 62-63 -0.07147371726349405 1104 64-65 0.09359252800559403 1104 66-67 -0.06677845334206012 1104 68-69 0.05960134657036775 1104 70-71 0.033198819482858966 1104 72-73 0.016671830849695368 1104 74-75 0.010963060274949754 1104 76-77 0.08323019297705869 1104 78-79 0.161361391787608 1104 80-81 0.1892887984040783 1104 82-83 0.1908995231094579 1104 84-85 0.33230479055655593 1104 86-87 0.16806184474071273 1104 88-89 0.25709577460673927 1104 90-91 0.13872179527442086 1104 92-93 0.24157811665066475 1104 94-95 0.2171941882159203 1104 96-97 0.13066715039921917 1104 98-99 0.2443371280570652 1104 100-101 0.32892649553537723 1105 1 0.395315282525889 1105 2 0.4314127342062761 1105 3 0.2042327751233799 1105 4 0.10510987187740994 1105 5 -0.0353398733964454 1105 6 0.10056720493014382 1105 7 0.12600194533452225 1105 8 0.02159009937648193 1105 9 0.03439322728490879 1105 10-11 0.07657789912555302 1105 12-13 -0.004579922863690911 1105 14-15 0.15776714422943883 1105 16-17 0.24655527654368825 1105 18-19 0.22999735671049137 1105 20-21 0.15016305153054788 1105 22-23 0.09617609413265171 1105 24-25 0.022409244041675436 1105 26-27 0.027922036313860588 1105 28-29 -0.003864765595999131 1105 30-31 0.008049483113879319 1105 32-33 0.07761634146724816 1105 34-35 0.10991234343964607 1105 36-37 0.1356889495556075 1105 38-39 0.058592457056853675 1105 40-41 -0.004883534345204055 1105 42-43 -0.024817552604055493 1105 44-45 -0.08716748728785007 1105 46-47 0.14004354071984437 1105 48-49 0.1981382989133138 1105 50-51 0.11189725594781663 1105 52-53 0.1132387930476284 1105 54-55 0.16974568394920908 1105 56-57 0.20081353021571857 1105 58-59 0.20530119643470357 1105 60-61 0.14479889022177161 1105 62-63 0.046998764750959765 1105 64-65 0.19066695931808653 1105 66-67 0.09950710253630746 1105 68-69 0.007906951220441272 1105 70-71 0.03151155929162641 1105 72-73 -0.13560815960489592 1105 74-75 -0.19446473004151343 1105 76-77 -0.08901902411851026 1105 78-79 -0.06326441352087997 1105 80-81 -0.11449314295311197 1105 82-83 0.12893468134781472 1105 84-85 0.28181517869894535 1105 86-87 0.12080524497497436 1105 88-89 0.11396278446657249 1105 90-91 -0.08745044472500041 1105 92-93 -0.03431525471864205 1105 94-95 0.07052425894958247 1105 96-97 -0.08629365095079322 1105 98-99 0.023118702414468117 1105 100-101 0.39831873022951925 1106 1 0.29719156662653745 1106 2 0.365281797729196 1106 3 0.11628561853844843 1106 4 0.0024612847336200616 1106 5 -0.04090173200421532 1106 6 0.0022741950443005976 1106 7 0.01918270458761384 1106 8 0.0271131305976553 1106 9 0.10334984327415953 1106 10-11 -0.042159128249728894 1106 12-13 0.0775136761454931 1106 14-15 0.07028073381474798 1106 16-17 0.13510068747563508 1106 18-19 -0.10872807509610993 1106 20-21 -0.034661560557537996 1106 22-23 0.08541950960017175 1106 24-25 -0.02653613174819469 1106 26-27 0.08724403117285107 1106 28-29 0.05914961501505189 1106 30-31 -0.0677925776068875 1106 32-33 -0.02783053213842379 1106 34-35 -0.23891289778372027 1106 36-37 0.009989233982665269 1106 38-39 0.16404097589076372 1106 40-41 0.2021491912188722 1106 42-43 -0.028109901108145152 1106 44-45 0.05337235158507525 1106 46-47 0.046412943159786835 1106 48-49 -0.012373179515108745 1106 50-51 0.0871995008288522 1106 52-53 0.2657212614954432 1106 54-55 0.04765633950027137 1106 56-57 -0.050936910376400135 1106 58-59 -0.06566238413475389 1106 60-61 0.16735949384943893 1106 62-63 -0.09182880282514105 1106 64-65 0.11119930911203824 1106 66-67 0.030878791843512943 1106 68-69 0.1475921762810799 1106 70-71 0.19748983503977158 1106 72-73 0.274292396219014 1106 74-75 0.4059710251116684 1106 76-77 0.2162246967033674 1106 78-79 0.22867582718259172 1106 80-81 0.20987619170342597 1106 82-83 0.22093962887819885 1106 84-85 0.24038668624133308 1106 86-87 0.2143256122425683 1106 88-89 0.08332254718517262 1106 90-91 0.13788676125625798 1106 92-93 0.5136781920082107 1106 94-95 0.676748338378566 1106 96-97 0.5255343226676459 1106 98-99 0.18349579859977538 1106 100-101 0.3572827892313555 1107 1 0.6486819174686609 1107 2 0.5859316809341237 1107 3 0.4013434163944467 1107 4 0.24750606093826377 1107 5 0.1742125215638879 1107 6 0.23672888483772425 1107 7 0.1890802733082353 1107 8 0.18441239441744983 1107 9 0.3943030795543052 1107 10-11 0.38434171701536 1107 12-13 0.37898989164239794 1107 14-15 0.5571873348781793 1107 16-17 0.4493983035996365 1107 18-19 0.48941155641399803 1107 20-21 0.3939378123471471 1107 22-23 0.41513321888609056 1107 24-25 0.3827677810295782 1107 26-27 0.4110847900838266 1107 28-29 0.5540201980766213 1107 30-31 0.5182552655393309 1107 32-33 0.4793895594700359 1107 34-35 0.3944647929292131 1107 36-37 0.4436523444439686 1107 38-39 0.47413439767233 1107 40-41 0.4903788748727109 1107 42-43 0.4824505581906209 1107 44-45 0.42446014492919204 1107 46-47 0.6458508133202159 1107 48-49 0.7173729411179295 1107 50-51 0.6954187757284771 1107 52-53 0.41064959957863323 1107 54-55 0.653930933573136 1107 56-57 0.6651200564926327 1107 58-59 0.5628703813098923 1107 60-61 0.5611598599405951 1107 62-63 0.40606695828466144 1107 64-65 0.5228227035367112 1107 66-67 0.585758555740405 1107 68-69 0.5426954214136686 1107 70-71 0.4538896482818835 1107 72-73 0.5850251211137838 1107 74-75 0.739037595448579 1107 76-77 0.6268211368808743 1107 78-79 0.7806092996800658 1107 80-81 0.8975218285935362 1107 82-83 0.7561994622239041 1107 84-85 0.7454489959852957 1107 86-87 1.0036390056963356 1107 88-89 1.1056885184118919 1107 90-91 0.9402282312179366 1107 92-93 1.2505587354908165 1107 94-95 1.2487161640483535 1107 96-97 1.164426225827679 1107 98-99 1.099969115637009 1107 100-101 1.133074083000949 1108 1 0.25284066312037723 1108 2 0.26120009693920565 1108 3 0.11651033937096855 1108 4 0.11624964136943561 1108 5 -0.10897416659118875 1108 6 -0.16271584019192886 1108 7 -0.15029147434666612 1108 8 -0.06601888711291792 1108 9 -0.052237872329428114 1108 10-11 -0.16709428023805373 1108 12-13 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-2.182503948741026 2316 74-75 -2.2515934059850053 2316 76-77 -1.9962738071132584 2316 78-79 -1.858732905631701 2316 80-81 -2.006727975303285 2316 82-83 -1.8148259545517078 2316 84-85 -2.0154178251595845 2316 86-87 -2.000055022247995 2316 88-89 -1.9361509594878044 2316 90-91 -2.1598557700683827 2316 92-93 -2.472971091728006 2316 94-95 -2.522115854416903 2316 96-97 -2.6142078441323164 2316 98-99 -2.970187736854328 2316 100-101 -3.525363387884113 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 9.0 4 23.0 5 51.0 6 162.0 7 304.0 8 768.0 9 1421.0 10 2453.0 11 3880.0 12 5566.0 13 7408.0 14 9467.0 15 12713.0 16 15659.0 17 19678.0 18 17386.0 19 13006.0 20 12573.0 21 13307.0 22 14495.0 23 16297.0 24 19471.0 25 23804.0 26 29513.0 27 36380.0 28 46301.0 29 57066.0 30 70850.0 31 88409.0 32 109752.0 33 141022.0 34 183721.0 35 250458.0 36 356053.0 37 461028.0 38 285945.0 39 18069.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.947401879550995 19.02182322049275 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28.268624637097915 28.39914462489046 21.527416555180846 20-21 23.37232865949603 25.76768982656627 27.974692776914516 22.88528873702318 22-23 22.426459924689777 25.9496100554675 28.482372732716954 23.141557287125774 24-25 23.610037070227843 26.096079751960893 28.203337301538216 22.090545876273048 26-27 22.912395088184148 25.375106260735375 29.63899288069068 22.073505770389797 28-29 21.748826706601793 27.861340883586006 28.520466681367935 21.869365728444265 30-31 22.35783454590357 25.94506474600432 28.224109595818923 23.472991112273185 32-33 22.430976320387856 26.333451133860287 28.63459236290013 22.60098018285173 34-35 23.8268392211928 25.831546494561426 28.21380387868065 22.127810405565125 36-37 23.177133097649534 25.311936952308155 29.248365455488525 22.262564494553793 38-39 22.373209211066502 26.55634287176698 29.020105887810104 22.05034202935641 40-41 22.74091763460844 26.78450812576287 28.20141663124484 22.27315760838385 42-43 22.166822903835754 26.906066801254592 27.27354942053607 23.653560874373586 44-45 23.00221954870669 25.63200193823952 27.653951732973383 23.711826780080408 46-47 23.985687800485703 26.85038837740546 27.38849278514404 21.775431036964793 48-49 23.512994759267794 26.602938657774494 27.435897490587006 22.448169092370705 50-51 21.81016018431572 26.966581366121044 28.728355048752256 22.49490340081098 52-53 23.2421490941648 27.70314164197319 26.537411742224382 22.51729752163763 54-55 22.385511968490704 26.469405088394875 27.39379412225167 23.75128882086275 56-57 23.46393176480424 26.24773819060367 27.136466125965185 23.1518639186269 58-59 24.740810757089367 26.043581669884226 26.618306570563544 22.597301002462864 60-61 23.078010817257706 26.697370803641757 28.536526427077995 21.688091952022546 62-63 22.44906969006731 26.818263250838175 28.117753947738848 22.614913111355666 64-65 22.12340678685921 28.10744420594404 27.229751824250776 22.539397182945972 66-67 22.85567673444874 26.740868359000437 26.52891430384887 23.874540602701956 68-69 22.459024120068662 26.713567333352245 27.291355338556855 23.53605320802224 70-71 23.724276307202505 26.563533031853314 26.848911080456944 22.86327958048723 72-73 23.276059525632455 25.747066819821463 28.37563644475572 22.601237209790362 74-75 22.258366393413603 28.379560574270762 26.865806287052635 22.496266745263 76-77 22.83653302724784 26.53672961978602 26.85072744357615 23.77600990938999 78-79 22.990772294316525 26.776642086042067 27.204572324646826 23.028013294994583 80-81 23.868199346824166 26.540563473473984 26.833022942209233 22.758214237492616 82-83 23.275939664553757 26.24226748930641 27.90049403603251 22.581298810107324 84-85 22.66338430982525 27.16849159251539 27.45664339199245 22.711480705666904 86-87 22.80264096654854 27.397351141692273 26.81001229621398 22.98999559554521 88-89 22.36850947215632 27.523919053345423 25.980820384757088 24.12675108974117 90-91 23.00810557534823 26.155940970540094 26.679751804752843 24.15620164935883 92-93 23.822759994761995 26.870209601335603 26.96483923625759 22.342191167644813 94-95 23.074919825651186 26.407754068864197 27.28969323447083 23.227632871013785 96-97 21.61453806460447 26.55191911504836 28.58728825832575 23.246254562021416 98-99 22.486110494937325 27.404748538530576 26.43845533005491 23.670685636477184 100-101 21.823907822384893 26.317417252161746 27.12923977356591 24.72943515188745 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 159.0 1 136.5 2 139.0 3 168.0 4 150.0 5 149.5 6 271.0 7 525.5 8 853.5 9 1055.5 10 1057.5 11 1048.0 12 1135.5 13 1295.0 14 1458.5 15 1609.0 16 1839.0 17 2070.0 18 2252.0 19 2479.0 20 2817.5 21 3200.0 22 3797.5 23 4623.0 24 5521.5 25 6964.0 26 9133.0 27 11913.5 28 14923.0 29 18477.5 30 23126.5 31 28180.5 32 34122.0 33 38860.0 34 42402.5 35 46234.5 36 51954.5 37 57265.0 38 59304.5 39 61248.0 40 63491.5 41 66042.0 42 67968.5 43 70798.5 44 74271.0 45 77452.0 46 80840.5 47 106112.0 48 155932.5 49 149837.5 50 101513.5 51 81215.0 52 75741.5 53 74369.0 54 73477.0 55 69169.0 56 63541.5 57 58195.5 58 53897.0 59 48510.5 60 40589.5 61 33242.0 62 27441.5 63 21575.5 64 15862.0 65 12187.0 66 9277.5 67 7061.0 68 5201.0 69 3660.5 70 2808.0 71 2174.0 72 1658.0 73 1392.0 74 1182.5 75 946.0 76 666.0 77 426.0 78 281.0 79 195.0 80 145.5 81 103.5 82 68.0 83 41.0 84 22.5 85 14.5 86 9.5 87 7.5 88 5.0 89 2.0 90 2.5 91 2.5 92 1.5 93 1.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.0336668985599724 2 0.012796074505570345 3 0.0049478154754872 4 0.01322261032242269 5 0.0 6 0.0 7 0.0 8 0.0 9 0.004222704586838214 10-11 2.772482809540241E-4 12-13 3.199018626392586E-4 14-15 2.1326790842617242E-5 16-17 0.0 18-19 2.1326790842617242E-5 20-21 0.0 22-23 0.001407568195612738 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.00471322077621841 34-35 5.118429802228137E-4 36-37 0.003625554443244931 38-39 2.772482809540241E-4 40-41 0.004457299286107003 42-43 0.011793715335967334 44-45 0.003135038253864734 46-47 0.0015142021498258241 48-49 0.01004491848687272 50-51 5.758233527506655E-4 52-53 0.0024739077377436 54-55 0.01160177421838378 56-57 0.004563933240320089 58-59 0.0018341040124650828 60-61 0.006803246278794899 62-63 0.0037961687699858684 64-65 0.018106445425382037 66-67 0.003966783096726807 68-69 4.4786260769496205E-4 70-71 0.001492875358983207 72-73 0.0 74-75 0.0 76-77 0.0011089931238160964 78-79 0.011879022499337803 80-81 0.016357648576287423 82-83 0.013137303159052219 84-85 0.009319807598223734 86-87 0.010727375793836473 88-89 0.028940455173431596 90-91 0.02510163282176049 92-93 0.002815136391225476 94-95 0.0086586770821026 96-97 0.010684722212151238 98-99 0.010791356166364324 100-101 0.01275342092388511 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2344469.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.277180173057545 #Duplication Level Percentage of deduplicated Percentage of total 1 75.14923753762255 37.78291755546948 2 14.060468821567104 14.138414485191747 3 4.607580697247926 6.94968494632308 4 2.0306639153961017 4.083842221811851 5 1.0962010470958008 2.7556948775364956 6 0.6550670067003588 1.9760953152779654 7 0.4497278865994929 1.5827734988387767 8 0.32148015890653386 1.293049269712558 9 0.2333144649124941 1.055735405044738 >10 1.2456813664305968 11.493157518966349 >50 0.09707735552578232 3.3396464357045823 >100 0.047521084960083296 4.243022135337946 >500 0.002818509745159075 0.956434492506957 >1k 0.0019644158829896083 2.107866023153059 >5k 8.540938621693949E-4 3.037290355565405 >10k+ 3.4163754486775795E-4 3.204375463559142 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 34214 1.4593496437786126 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 16112 0.687234508112498 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 12603 0.5375630899790101 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 11693 0.49874833064544677 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 9671 0.41250278847790267 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 9117 0.3888727042242828 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 8699 0.37104350707985473 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 7873 0.33581164860785107 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 7145 0.30475984114100035 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 6133 0.2615944164755431 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 6123 0.26116788065869073 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5828 0.24858507406154656 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5082 0.21676550212436163 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 5060 0.21582712332728649 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 4105 0.17509295281788753 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3623 0.15453392644560454 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3497 0.14915957515326497 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3414 0.14561932787339052 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 3203 0.13661942213780603 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2944 0.1255721444813303 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2919 0.12450580493919945 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2780 0.11857695708495186 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2717 0.11588978143878209 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2580 0.11004624074790496 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 8.530716337046897E-5 0.0 5 0.0 0.0 0.0 8.530716337046897E-5 0.0 6 0.0 0.0 0.0 1.2796074505570345E-4 0.0 7 0.0 0.0 0.0 1.2796074505570345E-4 0.0 8 0.0 0.0 0.0 1.2796074505570345E-4 0.0 9 0.0 0.0 0.0 1.2796074505570345E-4 0.0 10-11 0.0 0.0 0.0 1.9194111758355517E-4 0.0 12-13 0.0 0.0 0.0 2.132679084261724E-4 0.0 14-15 0.0 4.2653581685234485E-5 0.0 2.132679084261724E-4 0.0 16-17 0.0 8.530716337046897E-5 0.0 2.132679084261724E-4 0.0 18-19 0.0 8.530716337046897E-5 0.0 2.9857507179664137E-4 0.0 20-21 0.0 8.530716337046897E-5 0.0 3.8388223516711034E-4 0.0 22-23 0.0 8.530716337046897E-5 0.0 3.8388223516711034E-4 0.0 24-25 0.0 8.530716337046897E-5 0.0 5.118429802228138E-4 0.0 26-27 0.0 8.530716337046897E-5 0.0 5.33169771065431E-4 0.0 28-29 0.0 8.530716337046897E-5 0.0 7.03784097806369E-4 0.0 30-31 0.0 8.530716337046897E-5 0.0 0.0012582806597144173 0.0 32-33 0.0 8.530716337046897E-5 0.0 0.0018554308033076998 0.0 34-35 0.0 8.530716337046897E-5 0.0 0.0023672737835305135 0.0 36-37 0.0 8.530716337046897E-5 0.0 0.0032629989989204377 0.0 38-39 0.0 8.530716337046897E-5 0.0 0.004393318913579152 0.0 40-41 0.0 1.0663395421308621E-4 0.0 0.006248749716886852 0.0 42-43 0.0 1.2796074505570345E-4 0.0 0.008978578944741859 0.0 44-45 0.0 1.2796074505570345E-4 0.0 0.015952439550277697 0.0 46-47 0.0 1.2796074505570345E-4 0.0 0.03184089872802754 0.0 48-49 0.0 1.2796074505570345E-4 0.0 0.0421843922866969 0.0 50-51 0.0 1.2796074505570345E-4 0.0 0.05551363656333268 0.0 52-53 0.0 1.2796074505570345E-4 0.0 0.07082627238833186 0.0 54-55 0.0 1.2796074505570345E-4 0.0 0.08865546953275986 0.0 56-57 0.0 1.2796074505570345E-4 0.0 0.10961970493105261 0.0 58-59 0.0 1.2796074505570345E-4 0.0 0.1416312179858211 0.0 60-61 0.0 1.2796074505570345E-4 0.0 0.1792303502413553 0.0 62-63 0.0 1.2796074505570345E-4 0.0 0.23101179840722996 0.0 64-65 0.0 1.2796074505570345E-4 0.0 0.2698052309499507 0.0 66-67 0.0 1.2796074505570345E-4 0.0 0.3391812815609846 0.0 68-69 0.0 1.2796074505570345E-4 0.0 0.42728225453183644 0.0 70-71 0.0 1.2796074505570345E-4 0.0 0.484693975480162 0.0 72-73 0.0 1.2796074505570345E-4 0.0 0.5802379984550873 0.0 74-75 0.0 1.2796074505570345E-4 0.0 0.65987223546142 0.0 76-77 0.0 1.2796074505570345E-4 0.0 0.7273928552691462 0.0 78-79 0.0 1.2796074505570345E-4 0.0 0.8164748606187584 0.0 80-81 0.0 1.2796074505570345E-4 0.0 0.9111018315874512 0.0 82-83 0.0 1.2796074505570345E-4 0.0 1.0353517150365392 0.0 84-85 0.0 1.2796074505570345E-4 0.0 1.2222597099812367 0.0 86-87 0.0 1.2796074505570345E-4 0.0 1.4810176632747116 0.0 88-89 0.0 1.2796074505570345E-4 0.0 1.793071266883887 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 10435 0.0 23.184752 1 TACATGG 10750 0.0 22.051552 2 ACATGGG 10605 0.0 21.589687 3 GAGTACT 7530 0.0 21.505243 12-13 GTACTTT 7890 0.0 20.433516 14-15 TAAGGTG 900 0.0 20.051178 5 GTGTAAG 1015 0.0 19.863127 1 TATCACG 120 0.004761559 19.794102 2 AGTACTT 7970 0.0 19.424246 12-13 ACCGTAC 125 0.005677095 19.201023 1 AGAGTAC 13630 0.0 18.413599 10-11 CATGGGA 5240 0.0 17.948385 4 ACTTTTT 9160 0.0 17.833963 16-17 CATGGGG 5185 0.0 17.039454 4 GAGTACA 8675 0.0 16.987648 1 GTGTAGC 1335 0.0 16.899776 1 TACTTTT 9120 0.0 16.740423 14-15 GTAAGGT 890 0.0 16.544863 4 AGGTAAG 955 0.0 16.415632 2 AGTACAT 8375 0.0 16.222893 2 >>END_MODULE