FastQCFastQC Report
Thu 26 May 2016
SRR936505_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936505_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2325229
Sequences flagged as poor quality0
Sequence length101
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT303271.304258634310857No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT185620.7982869644237192No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT137350.590694507938788No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC119820.5153040840278528No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT92360.39720818895687265No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT83770.36026559104501105No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC74190.3190653479721782No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC71240.30637842552281946No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC62460.2686187037921856No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG60510.2602324330205756No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA57990.2493947907926488No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA45360.19507756010268237No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44760.19249716909603312No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37390.160801366231025No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT37240.16015626847936268No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA36800.15826398174115325No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC35280.15172699119097516No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC33070.14222255098315048No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG31730.1364596777349672No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC27660.11895602540652984No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC26590.11435432811133871No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT26230.11280609350734917No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG25390.10919354609804023No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG104400.025.492721
TACATGG107900.024.2269042
ACATGGG107400.023.5431213
CATGGGG51250.021.7950924
GAGTACT57450.020.1052912-13
AGAGTAC115200.018.75219310-11
GTACTTT61850.018.67459914-15
GTATAGG7250.018.3547571
CATGGGA54300.018.1198754
AGTACTT61850.017.8296312-13
GTGTAGC13650.017.756861
TATTCCG2453.739524E-617.461435
GAGTACA82000.017.4454141
TAAGGTG8350.017.0780455
AGTACAT79900.016.5964092
ACTTTTT72150.016.43438516-17
ATGGGGG26700.016.0226635
CTAGTAC5053.6379788E-1215.9997883
TACCTGG15100.015.7380082
GTAAGCG3200.015.58816794-95
Produced by FastQC (version 0.11.5)