##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936505_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2325229 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.364452705518467 31.0 31.0 34.0 30.0 34.0 2 31.378610450841617 31.0 31.0 34.0 28.0 34.0 3 31.452740353745803 31.0 31.0 34.0 28.0 34.0 4 35.04694247319296 37.0 35.0 37.0 32.0 37.0 5 35.02991705333109 37.0 35.0 37.0 32.0 37.0 6 35.17474493910062 37.0 35.0 37.0 32.0 37.0 7 35.03741652972675 37.0 35.0 37.0 32.0 37.0 8 34.97607762504252 37.0 35.0 37.0 32.0 37.0 9 36.43338914145661 39.0 37.0 39.0 32.0 39.0 10-11 36.35042720523441 39.0 37.0 39.0 32.0 39.0 12-13 36.2573815310234 39.0 35.0 39.0 32.0 39.0 14-15 37.29747156086562 40.0 37.0 41.0 32.0 41.0 16-17 37.200304357119236 39.0 36.5 41.0 31.0 41.0 18-19 37.14433030037041 39.0 36.0 41.0 31.0 41.0 20-21 37.03151926111364 39.0 36.0 41.0 31.0 41.0 22-23 36.90855266298502 39.0 36.0 41.0 30.5 41.0 24-25 37.04572366850749 39.0 36.0 41.0 31.0 41.0 26-27 37.03458885124863 39.0 36.0 41.0 30.5 41.0 28-29 36.96979415790875 39.0 36.0 41.0 30.0 41.0 30-31 36.834195255607085 39.0 36.0 41.0 30.0 41.0 32-33 36.67436583665523 39.0 36.0 41.0 30.0 41.0 34-35 36.43765001210633 39.0 35.0 40.5 30.0 41.0 36-37 36.241319027072166 39.0 35.0 40.0 30.0 41.0 38-39 36.05272921505795 38.5 35.0 40.0 29.0 41.0 40-41 35.945510958275506 38.0 35.0 40.0 29.0 41.0 42-43 35.71717172803194 38.0 35.0 40.0 27.5 41.0 44-45 35.47377011038483 38.0 34.5 40.0 27.0 41.0 46-47 35.2203393730252 38.0 34.0 40.0 26.0 41.0 48-49 35.138031135858014 38.0 34.0 40.0 26.0 41.0 50-51 34.2019663439601 37.5 33.0 39.5 25.0 40.5 52-53 34.37200873548369 37.5 33.5 39.5 25.0 40.5 54-55 34.81291778143142 38.0 34.0 40.0 26.0 41.0 56-57 34.67513995395723 38.0 34.0 40.0 25.5 41.0 58-59 34.396478368367156 38.0 34.0 40.0 24.5 41.0 60-61 34.004347313748454 37.0 33.0 40.0 23.0 41.0 62-63 33.713239642202986 37.0 33.0 40.0 23.0 41.0 64-65 33.387560537048174 36.5 32.5 39.0 22.0 41.0 66-67 32.927371669629096 36.0 32.0 39.0 21.5 41.0 68-69 32.493922964146755 35.0 31.5 39.0 20.5 40.0 70-71 31.99503231724703 35.0 31.0 38.0 19.0 40.0 72-73 31.587736734747416 35.0 31.0 37.0 18.0 39.5 74-75 31.192934760404242 35.0 31.0 37.0 17.5 39.0 76-77 30.786258901811394 34.5 30.0 36.0 13.5 39.0 78-79 30.317797300824992 34.0 30.0 36.0 9.5 38.0 80-81 29.787117526918856 34.0 29.0 35.0 7.0 37.0 82-83 29.28737621111727 34.0 29.0 35.0 4.0 37.0 84-85 28.963950002343857 34.0 29.0 35.0 2.0 36.0 86-87 28.731149921147555 34.0 29.0 35.0 2.0 36.0 88-89 28.304692570065143 34.0 28.0 35.0 2.0 35.5 90-91 27.873671797487475 33.5 27.0 35.0 2.0 35.0 92-93 27.484357669717692 33.0 26.5 35.0 2.0 35.0 94-95 26.996564424407232 33.0 25.0 35.0 2.0 35.0 96-97 26.405307606261577 33.0 25.0 35.0 2.0 35.0 98-99 25.7416669497929 33.0 23.5 35.0 2.0 35.0 100-101 23.240566628061153 29.5 9.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5625541052164458 1101 2 0.6242089444975569 1101 3 0.611674303083209 1101 4 0.6276277049313563 1101 5 0.4971665654327637 1101 6 0.480591122562231 1101 7 0.5316893830510239 1101 8 0.6674722292102899 1101 9 0.8643560455691173 1101 10-11 0.7052506779614092 1101 12-13 0.8533401013974995 1101 14-15 1.1015865973382262 1101 16-17 1.039584692764194 1101 18-19 1.1143781679181544 1101 20-21 1.0861738203062359 1101 22-23 1.0245144892585358 1101 24-25 1.0419934893307499 1101 26-27 0.9493489973717004 1101 28-29 1.0070678307354797 1101 30-31 0.937330689007041 1101 32-33 0.9420893926648759 1101 34-35 0.982267234706562 1101 36-37 0.8596266622664572 1101 38-39 0.963979717558054 1101 40-41 0.9066058481431227 1101 42-43 1.0020412068990794 1101 44-45 0.9066675170903338 1101 46-47 1.0302010877896493 1101 48-49 1.0613879770290922 1101 50-51 1.0694961432477257 1101 52-53 1.1771774737793308 1101 54-55 1.2424196142540183 1101 56-57 1.054246424151124 1101 58-59 0.934973367836406 1101 60-61 0.9976292527892312 1101 62-63 1.1523088593167543 1101 64-65 1.076114439987201 1101 66-67 1.074318590971302 1101 68-69 1.1156009622275533 1101 70-71 0.9814600452540887 1101 72-73 0.9700461353334902 1101 74-75 0.8805834777155646 1101 76-77 0.9518784072852107 1101 78-79 0.8710965859025137 1101 80-81 1.084127515486852 1101 82-83 1.2143216895758648 1101 84-85 1.1523164075908774 1101 86-87 1.126570217583886 1101 88-89 1.1707385192210467 1101 90-91 1.1461931686593019 1101 92-93 1.093969635077979 1101 94-95 1.0899384479088319 1101 96-97 0.7938995885950497 1101 98-99 0.7960291768338017 1101 100-101 1.2137776892707315 1102 1 0.4510602446568086 1102 2 0.5708130546568029 1102 3 0.41823055915351404 1102 4 0.5977681741090635 1102 5 0.6040721161519116 1102 6 0.490406850559296 1102 7 0.5796978337217169 1102 8 0.6276418280668778 1102 9 0.7373403418484159 1102 10-11 0.721684738473158 1102 12-13 0.6232199314707998 1102 14-15 0.7730912651707058 1102 16-17 0.7378871235029081 1102 18-19 0.9083884838093965 1102 20-21 0.9847177656867743 1102 22-23 0.7442566134438167 1102 24-25 0.6818273641010393 1102 26-27 0.7335818341910567 1102 28-29 0.8008596789137883 1102 30-31 0.634687922454475 1102 32-33 0.6894676337490253 1102 34-35 0.5554488447013952 1102 36-37 0.5809509454357382 1102 38-39 0.5084220413988234 1102 40-41 0.5562973100658297 1102 42-43 0.756085786801961 1102 44-45 0.4495022151882466 1102 46-47 0.5483468852768141 1102 48-49 0.7286162784235941 1102 50-51 0.8026812034517192 1102 52-53 0.8109105474102662 1102 54-55 0.8498394764134716 1102 56-57 0.9095035144968406 1102 58-59 0.9725953814198434 1102 60-61 0.7432437500685793 1102 62-63 0.8276303210423563 1102 64-65 0.7686335746950377 1102 66-67 0.8907718540046687 1102 68-69 0.7888160010024023 1102 70-71 0.6727837059025958 1102 72-73 0.6858747700010213 1102 74-75 0.7057801328080338 1102 76-77 0.8262228821791808 1102 78-79 0.7177883563535374 1102 80-81 1.0164454569453092 1102 82-83 1.1030199518061998 1102 84-85 0.9700503266011218 1102 86-87 0.826102761076914 1102 88-89 0.871550352657902 1102 90-91 0.954093796390513 1102 92-93 0.8508267794405491 1102 94-95 1.0662856833604302 1102 96-97 1.061252940557175 1102 98-99 1.0900496403990658 1102 100-101 1.1334597275169251 1103 1 0.2955456915539969 1103 2 0.38883288623449985 1103 3 0.3487170334756371 1103 4 0.5645563739863206 1103 5 0.6097595270616125 1103 6 0.38624469730354605 1103 7 0.3115837779851276 1103 8 0.5568327318668054 1103 9 0.6644045544137853 1103 10-11 0.628411684716589 1103 12-13 0.49450575582442724 1103 14-15 0.4344605169511979 1103 16-17 0.5778099901266955 1103 18-19 0.47628856421575705 1103 20-21 0.5832092088194756 1103 22-23 0.5000109674508622 1103 24-25 0.6011726410159355 1103 26-27 0.626742236943123 1103 28-29 0.2632764712095579 1103 30-31 0.4417702315817422 1103 32-33 0.5256606269199935 1103 34-35 0.36890730891198587 1103 36-37 0.39084439814890715 1103 38-39 0.4480275184358007 1103 40-41 0.3338851902583073 1103 42-43 0.2838218748134267 1103 44-45 0.45713802010738647 1103 46-47 0.47409798682897986 1103 48-49 0.5189722365645153 1103 50-51 0.5699255120755424 1103 52-53 0.5796435585583666 1103 54-55 0.5435792378516027 1103 56-57 0.7622149851755822 1103 58-59 0.4074413208614871 1103 60-61 0.3403016312631024 1103 62-63 0.35825676475072754 1103 64-65 0.5360127025183843 1103 66-67 0.2886096899260693 1103 68-69 0.6121365129832057 1103 70-71 0.92347231012549 1103 72-73 0.894914784526037 1103 74-75 0.6059922794487775 1103 76-77 0.5651760582116303 1103 78-79 0.679471900144776 1103 80-81 0.600930258203153 1103 82-83 0.42987795164161824 1103 84-85 0.44940358257342794 1103 86-87 0.5785661271083349 1103 88-89 0.6902338305790003 1103 90-91 0.9282704616035566 1103 92-93 0.6206478608342145 1103 94-95 0.7346685191316134 1103 96-97 0.8978379823623186 1103 98-99 0.9476438986126361 1103 100-101 1.4396617465767214 1104 1 0.2774369985720462 1104 2 0.3906825025778744 1104 3 0.2796774624290599 1104 4 0.3343942702437914 1104 5 0.45415343482287085 1104 6 0.3497005398172206 1104 7 0.42856816206646187 1104 8 0.3646742457088479 1104 9 0.5122101359220608 1104 10-11 0.5741719987896872 1104 12-13 0.47212369706714696 1104 14-15 0.5644786084551114 1104 16-17 0.40014279143420595 1104 18-19 0.6516419519501326 1104 20-21 0.8171376005556894 1104 22-23 0.5107172558957558 1104 24-25 0.5412049636434659 1104 26-27 0.1681708677149345 1104 28-29 0.5140278666582461 1104 30-31 0.5626172341110447 1104 32-33 0.38791228503927044 1104 34-35 0.20377694587509154 1104 36-37 0.2573324476219341 1104 38-39 0.2983674145349937 1104 40-41 0.40243711076168864 1104 42-43 0.40968764029496896 1104 44-45 0.4715820376101547 1104 46-47 0.32986434930865727 1104 48-49 0.383435555968731 1104 50-51 0.32082077652054153 1104 52-53 0.38943254114762027 1104 54-55 0.47138914169661916 1104 56-57 0.49447173914007436 1104 58-59 0.4772026541036283 1104 60-61 0.5841325430254827 1104 62-63 0.7758149954360292 1104 64-65 0.5778484100333401 1104 66-67 0.6416392289973771 1104 68-69 0.5944323900039521 1104 70-71 0.5568286636919169 1104 72-73 0.5024087388052436 1104 74-75 0.21079363638319037 1104 76-77 0.46654262567105675 1104 78-79 0.5002084449468498 1104 80-81 0.5430407581868586 1104 82-83 0.7237749160147509 1104 84-85 0.7592218403100297 1104 86-87 0.7663841921840309 1104 88-89 0.961606657727426 1104 90-91 0.8041202018515321 1104 92-93 0.7033391403497369 1104 94-95 0.7357628008627408 1104 96-97 0.7510530202266352 1104 98-99 1.1877380661080146 1104 100-101 1.5344712360933528 1105 1 0.34180676130462473 1105 2 0.2933670247058551 1105 3 0.383280585445231 1105 4 0.35591560460409255 1105 5 0.48041221329919637 1105 6 0.3941335511770916 1105 7 0.39041428897417774 1105 8 0.49826977988220733 1105 9 0.6298012224167948 1105 10-11 0.5845632371321656 1105 12-13 0.4808436886169858 1105 14-15 0.29202009985944244 1105 16-17 0.3219041614950342 1105 18-19 0.3961130138599884 1105 20-21 0.3561296668299221 1105 22-23 0.3569076107282143 1105 24-25 0.5457407238941627 1105 26-27 0.35259206514997743 1105 28-29 0.317682775285526 1105 30-31 0.2349265888041998 1105 32-33 0.23007933032101846 1105 34-35 0.18304170007775156 1105 36-37 0.19418900431477226 1105 38-39 0.2929788399082369 1105 40-41 0.1411451182491632 1105 42-43 0.37962203455396093 1105 44-45 0.2265460659281331 1105 46-47 0.2854585422311473 1105 48-49 0.3746673587849969 1105 50-51 0.5064852185208437 1105 52-53 0.39192744854140926 1105 54-55 0.5099608264870668 1105 56-57 0.5526973216573126 1105 58-59 0.6029050474070132 1105 60-61 0.46242421688459245 1105 62-63 0.49105554332140855 1105 64-65 0.5809059232535603 1105 66-67 0.7416209923807315 1105 68-69 0.7173373501155638 1105 70-71 0.793274196465191 1105 72-73 0.7546990380948841 1105 74-75 0.7954360834996628 1105 76-77 0.49438421486902584 1105 78-79 0.5207385509602531 1105 80-81 0.5040871500837731 1105 82-83 0.6407395596634622 1105 84-85 0.6575846919143054 1105 86-87 0.7335967403940948 1105 88-89 0.4872555108764054 1105 90-91 0.5810227559348853 1105 92-93 0.6578131725773311 1105 94-95 0.4603192647242871 1105 96-97 0.7725506948364789 1105 98-99 0.9149468145798814 1105 100-101 1.1002121123796194 1106 1 0.019459432232750373 1106 2 0.14151363736706912 1106 3 0.24170500238582804 1106 4 0.3766866567563625 1106 5 0.2936734429929473 1106 6 0.21124570500590067 1106 7 0.34876186089712036 1106 8 0.26845460689418843 1106 9 0.5532987982427073 1106 10-11 0.5027531293236294 1106 12-13 0.6236933724769287 1106 14-15 0.7068816313823802 1106 16-17 0.6718794427252561 1106 18-19 0.5846895596504211 1106 20-21 0.4384218340155357 1106 22-23 0.4669261714886872 1106 24-25 0.37798067809939084 1106 26-27 0.21304125289987041 1106 28-29 0.37361970339294004 1106 30-31 0.33245774746400514 1106 32-33 0.2647450198012322 1106 34-35 0.33255773258510146 1106 36-37 0.2878538004499731 1106 38-39 0.2940755726940054 1106 40-41 0.2914769568919695 1106 42-43 -0.12492725987694797 1106 44-45 0.26820898864077947 1106 46-47 0.2744703985673951 1106 48-49 0.37960191909446195 1106 50-51 0.37106877229934554 1106 52-53 0.44088136755058116 1106 54-55 0.6385095311367905 1106 56-57 0.31264181284094406 1106 58-59 0.32572472181310985 1106 60-61 0.4992367965701021 1106 62-63 0.5179512994989324 1106 64-65 0.3973168446131723 1106 66-67 0.39368123679298606 1106 68-69 0.3435828725489358 1106 70-71 0.502152658177117 1106 72-73 0.5295449577632212 1106 74-75 0.3814874118082905 1106 76-77 0.35649649879661993 1106 78-79 0.38721087894797535 1106 80-81 0.47244400238695405 1106 82-83 0.7490708258716019 1106 84-85 0.7317652863790336 1106 86-87 0.8290122325578118 1106 88-89 0.8469418860639664 1106 90-91 0.8875742707184422 1106 92-93 1.1590568302613988 1106 94-95 1.0308028852868354 1106 96-97 1.2438342494823047 1106 98-99 1.3799434753776474 1106 100-101 1.8060695067610872 1107 1 0.23265381636938187 1107 2 0.3126916499432575 1107 3 0.13504090885243514 1107 4 0.20993443340839235 1107 5 0.3127895717139353 1107 6 0.1979001492895236 1107 7 0.3174452207658902 1107 8 0.3017284365205981 1107 9 0.37555961746401323 1107 10-11 0.4835443678048392 1107 12-13 0.5151800002249516 1107 14-15 0.3595340493235497 1107 16-17 0.45360456776344904 1107 18-19 0.514581648873822 1107 20-21 0.6594914533526364 1107 22-23 0.7344977769630248 1107 24-25 0.5210957775670693 1107 26-27 0.4774736357821041 1107 28-29 0.4756117910081272 1107 30-31 0.6134696321233122 1107 32-33 0.599766167740313 1107 34-35 0.7143986486205449 1107 36-37 0.6141156810887551 1107 38-39 0.561611475944197 1107 40-41 0.4558458155465459 1107 42-43 0.43332140128965335 1107 44-45 0.5700169364061765 1107 46-47 0.6448600674373139 1107 48-49 0.6767848206238725 1107 50-51 0.6236263059510989 1107 52-53 0.6904186860240671 1107 54-55 0.7598377503677511 1107 56-57 0.6439711578177096 1107 58-59 0.7158298986356115 1107 60-61 0.7230271827009105 1107 62-63 0.6457515921928447 1107 64-65 0.6707719193144541 1107 66-67 0.6421121079594201 1107 68-69 0.5653863173310967 1107 70-71 0.7664435187293748 1107 72-73 0.7019580743100704 1107 74-75 0.6072745493583476 1107 76-77 0.4401300846186551 1107 78-79 0.6214716660051138 1107 80-81 0.7550589490748507 1107 82-83 0.8661530535617317 1107 84-85 0.900508149954728 1107 86-87 0.79191472688888 1107 88-89 0.5732935047256156 1107 90-91 0.710851316761449 1107 92-93 0.7081518914537241 1107 94-95 0.8902511090207703 1107 96-97 0.8245206327826722 1107 98-99 1.1948337151615043 1107 100-101 1.482493655616846 1108 1 0.013262027849986424 1108 2 0.09489170391044865 1108 3 0.00900382568419289 1108 4 0.23510342843696463 1108 5 0.2759342157864282 1108 6 0.08817613395373769 1108 7 0.07160034673251658 1108 8 0.11176835580995004 1108 9 0.2367185975978927 1108 10-11 -0.015240422547798005 1108 12-13 0.2203439460985166 1108 14-15 0.2847548945609404 1108 16-17 0.41857518597844745 1108 18-19 0.19556889454396043 1108 20-21 0.18312405812891797 1108 22-23 0.43115967884511264 1108 24-25 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2316 86-87 -3.3245614062840048 2316 88-89 -3.6638444176271854 2316 90-91 -3.5764613146348303 2316 92-93 -3.9704104945746757 2316 94-95 -4.250910811022013 2316 96-97 -5.326721817709913 2316 98-99 -6.863778804147881 2316 100-101 -7.143193552253134 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4869.0 3 1974.0 4 2049.0 5 2270.0 6 2455.0 7 2925.0 8 3475.0 9 4587.0 10 5771.0 11 7229.0 12 8747.0 13 10259.0 14 11641.0 15 13712.0 16 15870.0 17 19257.0 18 17307.0 19 14751.0 20 15548.0 21 17054.0 22 19041.0 23 21803.0 24 24728.0 25 29079.0 26 33581.0 27 39706.0 28 47464.0 29 56804.0 30 68417.0 31 83363.0 32 104300.0 33 132757.0 34 172603.0 35 232432.0 36 319717.0 37 405989.0 38 307314.0 39 44374.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.34077156686902 18.222180729801018 14.04116440162503 22.39588330170492 2 15.341737171843356 20.778938094820216 38.7285064603218 25.15081827301463 3 21.540809553695446 23.72869253107803 29.115766118226205 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23.474567688004676 26.72400650008182 27.037398694263704 22.764027117649803 50-51 21.725163564956993 27.19532707185504 28.41817752533843 22.66133183784953 52-53 23.14113779346721 27.826076110947678 26.241671440589183 22.79111465499593 54-55 22.254778286393105 26.586514950867702 27.147771939430093 24.0109348233091 56-57 23.410000464553693 26.361292870133155 26.767884887036026 23.460821778277126 58-59 24.65009000174197 26.197496295627225 26.35840263574994 22.794011066880866 60-61 23.088294688107673 26.756046251257555 28.250700121679877 21.904958938954895 62-63 22.310821228924134 27.065405206051828 27.86154899879897 22.76222456622507 64-65 22.02895802046079 28.343048509651524 26.856871670219007 22.771121799668677 66-67 22.71755834193174 26.934233044292082 26.175881478321045 24.17232713545513 68-69 22.366386651494132 26.84072213073344 27.045985819513774 23.746905398258658 70-71 23.701988448261144 26.64288852393516 26.480616247570808 23.17450678023289 72-73 23.112488691694356 25.903250577430526 28.218778700839913 22.7654820300352 74-75 22.128120584281717 28.484733536030326 26.711167107606816 22.675978772081145 76-77 22.644506286986623 26.7112472019786 26.659937150563174 23.984309360471606 78-79 22.95125680907327 26.9738544058798 26.941877486033484 23.133011299013447 80-81 23.791799496608213 26.774989376472025 26.49193902180963 22.941272105110134 82-83 23.20673299826329 26.460096529762772 27.651514825688167 22.681655646285773 84-85 22.68025162307811 27.3597836225628 27.205406510980495 22.754558243378593 86-87 22.728756525486492 27.66821325750049 26.733699939013743 22.86933027799927 88-89 22.337021830778138 27.658978282767027 25.731979221235008 24.272020665219827 90-91 22.916915756253186 26.432175521926908 26.50292413254462 24.147984589275286 92-93 23.883422773641747 27.03679584038045 26.788626943584642 22.291154442393157 94-95 23.028611109856605 26.63326384807266 27.10084309213066 23.237281949940076 96-97 21.624481262213738 26.877633473971656 28.427293053811525 23.070592210003078 98-99 22.473971014093763 27.737409644877054 26.25744392003152 23.53117542099766 100-101 21.981087429766216 26.612688114680044 26.974548051523417 24.431676404030323 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 156.0 1 116.0 2 108.5 3 154.5 4 151.0 5 144.5 6 240.5 7 457.0 8 694.5 9 853.0 10 895.0 11 870.0 12 947.5 13 1106.0 14 1241.0 15 1336.0 16 1450.5 17 1626.5 18 1816.0 19 2011.0 20 2250.5 21 2586.0 22 3129.0 23 3900.0 24 4858.5 25 6223.0 26 8268.0 27 11036.0 28 13835.5 29 17229.0 30 21969.0 31 27288.0 32 33112.0 33 37738.0 34 41404.0 35 45576.5 36 51636.5 37 57216.0 38 59241.0 39 60660.0 40 62709.5 41 65657.5 42 68356.5 43 71349.0 44 74588.0 45 78770.0 46 83760.5 47 106360.0 48 150370.0 49 146045.5 50 102953.0 51 82961.0 52 76445.0 53 74662.0 54 73464.0 55 68977.0 56 63468.5 57 57673.0 58 53404.5 59 48420.0 60 40588.0 61 33245.0 62 27447.0 63 21714.0 64 16060.5 65 12328.0 66 9385.0 67 7167.0 68 5320.0 69 3812.0 70 2896.5 71 2201.0 72 1657.5 73 1412.5 74 1180.5 75 913.0 76 683.5 77 437.5 78 284.0 79 197.0 80 129.5 81 86.0 82 65.0 83 50.0 84 33.0 85 19.0 86 14.0 87 10.0 88 8.5 89 9.0 90 7.5 91 5.0 92 4.0 93 4.0 94 3.0 95 2.0 96 1.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0362114871266443 2 0.02481476018061017 3 0.022191362657183446 4 0.05165082664976224 5 0.05844585630060523 6 0.0483823313746732 7 0.05156481361620727 8 0.04472677744858679 9 0.04442573183114438 10-11 0.05481180563290755 12-13 0.04909193890150175 14-15 0.04249043857615745 16-17 0.05210239507592586 18-19 0.056145007653009664 20-21 0.012901955033246188 22-23 0.022191362657183446 24-25 0.006171435157569427 26-27 0.011482739979589106 28-29 0.006666010100510531 30-31 0.009633459758157154 32-33 0.003999606060306318 34-35 0.008794832680996153 36-37 0.009504440207824691 38-39 0.012063327956085185 40-41 0.0018492802214319535 42-43 0.001978299771764415 44-45 0.0027524170737591868 46-47 3.4405213421989834E-4 48-49 0.0011826792113809005 50-51 2.5803910066492377E-4 52-53 0.004171632127416267 54-55 0.007741173019947713 56-57 0.0179337174962122 58-59 0.011783785597031518 60-61 0.011869798630586492 62-63 0.006300454707901889 64-65 4.300651677748729E-5 66-67 0.007999212120612637 68-69 0.017826201204268484 70-71 0.005354311338797168 72-73 0.0024728747147055195 74-75 0.0035910441509201894 76-77 0.006386467741456863 78-79 0.0049242461710222955 80-81 0.008644309872274946 82-83 0.006149931899180684 84-85 0.017310123002938636 86-87 0.004945749429411039 88-89 0.007010062234730429 90-91 0.004085619093861292 92-93 0.017245613227772405 94-95 0.011848295372197748 96-97 0.01008502818432077 98-99 0.02206234310685098 100-101 0.03651253274408671 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2325229.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.41757258174261 #Duplication Level Percentage of deduplicated Percentage of total 1 76.78837789883445 40.25060371946435 2 13.210392126328601 13.849133762302213 3 4.287338092356037 6.7419556691562725 4 1.9207493225251133 4.027240680991723 5 0.9816601627642406 2.5728121416149925 6 0.6266467444569281 1.9708380726410248 7 0.41180283448942373 1.51099934763517 8 0.28318780133791527 1.1875213704715426 9 0.2096780330097236 0.989173216267579 >10 1.1416369519884273 10.964886770013589 >50 0.09041969655232916 3.2452432636873545 >100 0.04297989505030235 4.012806281827961 >500 0.002234224004509082 0.8055611804246058 >1k 0.0019859768928969217 2.455504199064002 >5k 5.792432604282689E-4 2.1796823410885557 >10k+ 3.309961488161536E-4 3.236037983348977 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 30327 1.304258634310857 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 18562 0.7982869644237192 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 13735 0.590694507938788 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 11982 0.5153040840278528 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 9236 0.39720818895687265 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 8377 0.36026559104501105 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 7419 0.3190653479721782 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 7124 0.30637842552281946 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 6246 0.2686187037921856 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 6051 0.2602324330205756 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 5799 0.2493947907926488 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 4536 0.19507756010268237 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4476 0.19249716909603312 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3739 0.160801366231025 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3724 0.16015626847936268 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3680 0.15826398174115325 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3528 0.15172699119097516 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3307 0.14222255098315048 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 3173 0.1364596777349672 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2766 0.11895602540652984 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2659 0.11435432811133871 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2623 0.11280609350734917 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2539 0.10919354609804023 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 4.300651677748729E-5 0.0 9 0.0 0.0 0.0 8.601303355497459E-5 0.0 10-11 0.0 0.0 0.0 1.2901955033246189E-4 0.0 12-13 2.1503258388743646E-5 2.1503258388743646E-5 0.0 1.7202606710994917E-4 0.0 14-15 4.300651677748729E-5 4.300651677748729E-5 0.0 1.7202606710994917E-4 0.0 16-17 4.300651677748729E-5 4.300651677748729E-5 0.0 2.1503258388743646E-4 0.0 18-19 4.300651677748729E-5 4.300651677748729E-5 0.0 2.3653584227618013E-4 0.0 20-21 4.300651677748729E-5 4.300651677748729E-5 0.0 2.795423590536674E-4 0.0 22-23 4.300651677748729E-5 4.300651677748729E-5 0.0 3.0104561744241106E-4 0.0 24-25 4.300651677748729E-5 4.300651677748729E-5 0.0 3.8705865099738563E-4 0.0 26-27 4.300651677748729E-5 4.300651677748729E-5 0.0 4.5156842616361656E-4 0.0 28-29 4.300651677748729E-5 4.300651677748729E-5 0.0 7.526140436060276E-4 0.0 30-31 4.300651677748729E-5 4.300651677748729E-5 0.0 0.0013977117952683371 0.0 32-33 4.300651677748729E-5 4.300651677748729E-5 0.0 0.0021718290972631083 0.0 34-35 4.300651677748729E-5 4.300651677748729E-5 0.0 0.003225488758311547 0.0 36-37 4.300651677748729E-5 6.450977516623094E-5 0.0 0.004279148419359986 0.0 38-39 4.300651677748729E-5 1.0751629194371824E-4 0.0 0.005397317855574655 0.0 40-41 4.300651677748729E-5 1.5052280872120553E-4 0.0 0.00808522515416761 0.0 42-43 4.300651677748729E-5 1.7202606710994917E-4 0.0 0.011160191103757952 0.0 44-45 4.300651677748729E-5 1.7202606710994917E-4 0.0 0.018707834798206972 0.0 46-47 4.300651677748729E-5 1.7202606710994917E-4 0.0 0.03434070364682361 0.0 48-49 4.300651677748729E-5 1.9352932549869282E-4 0.0 0.045178345874750406 0.0 50-51 4.300651677748729E-5 2.1503258388743646E-4 0.0 0.059435006186487435 0.0 52-53 4.300651677748729E-5 2.1503258388743646E-4 0.0 0.07543343042771272 0.0 54-55 4.300651677748729E-5 2.1503258388743646E-4 0.0 0.09444231084336209 0.0 56-57 4.300651677748729E-5 2.1503258388743646E-4 0.0 0.12052576326890814 0.0 58-59 4.300651677748729E-5 2.1503258388743646E-4 0.0 0.15447940826473436 0.0 60-61 4.300651677748729E-5 2.5803910066492377E-4 0.0 0.1966257947066719 0.0 62-63 4.300651677748729E-5 2.5803910066492377E-4 0.0 0.25274929910129285 0.0 64-65 4.300651677748729E-5 2.795423590536674E-4 0.0 0.2930249020634097 0.0 66-67 4.300651677748729E-5 3.0104561744241106E-4 0.0 0.3660499675515831 0.0 68-69 4.300651677748729E-5 3.0104561744241106E-4 0.0 0.45969665783456165 0.0 70-71 4.300651677748729E-5 3.225488758311547E-4 0.0 0.5178629717761132 0.0 72-73 4.300651677748729E-5 3.4405213421989834E-4 0.0 0.6168854766562777 0.0 74-75 4.300651677748729E-5 3.4405213421989834E-4 0.0 0.7009847202146541 0.0 76-77 4.300651677748729E-5 3.4405213421989834E-4 0.0 0.7703542317767411 0.0 78-79 4.300651677748729E-5 3.4405213421989834E-4 0.0 0.8681080444119698 0.0 80-81 4.300651677748729E-5 3.4405213421989834E-4 0.0 0.970506560859167 2.1503258388743646E-5 82-83 6.450977516623094E-5 3.4405213421989834E-4 0.0 1.100106699168125 4.300651677748729E-5 84-85 8.601303355497459E-5 3.4405213421989834E-4 0.0 1.29017400006623 4.300651677748729E-5 86-87 8.601303355497459E-5 3.4405213421989834E-4 0.0 1.5487291789324837 4.300651677748729E-5 88-89 8.601303355497459E-5 3.4405213421989834E-4 0.0 1.8736864197031777 4.300651677748729E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 10440 0.0 25.49272 1 TACATGG 10790 0.0 24.226904 2 ACATGGG 10740 0.0 23.543121 3 CATGGGG 5125 0.0 21.795092 4 GAGTACT 5745 0.0 20.10529 12-13 AGAGTAC 11520 0.0 18.752193 10-11 GTACTTT 6185 0.0 18.674599 14-15 GTATAGG 725 0.0 18.354757 1 CATGGGA 5430 0.0 18.119875 4 AGTACTT 6185 0.0 17.82963 12-13 GTGTAGC 1365 0.0 17.75686 1 TATTCCG 245 3.739524E-6 17.46143 5 GAGTACA 8200 0.0 17.445414 1 TAAGGTG 835 0.0 17.078045 5 AGTACAT 7990 0.0 16.596409 2 ACTTTTT 7215 0.0 16.434385 16-17 ATGGGGG 2670 0.0 16.022663 5 CTAGTAC 505 3.6379788E-12 15.999788 3 TACCTGG 1510 0.0 15.738008 2 GTAAGCG 320 0.0 15.588167 94-95 >>END_MODULE